CmaCh17G010870 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G010870
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpectinesterase inhibitor 6-like
LocationCma_Chr17: 7687344 .. 7687871 (-)
RNA-Seq ExpressionCmaCh17G010870
SyntenyCmaCh17G010870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGCGTGCACCCAGTTCGAGAGCATCAATTACAATCATTTTTGCCCTCATCTCCATTCTCCCATGGCCGACACACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGCGTAACACAACACCAAGATCTCTGTATCCAATTACTGTCGCCATTTTCAAGCAGCGCCAAGCGAAGCCCCACGAAATGGGCGCGAACGGGCGTGTCGGTGACAATATCAGAGGCTAAAACCGTGGCGCGATTCCTGGGGAGGGTGAAGAACAACAAGCGGCTCATGGGGAGAAACAGAGCCGCAGTTTCCGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTCCACAGGTCGCTTGGGGTTCTTAGAAGACTGAGTAAGAGGAATTTTGACGCCCAAATGGGGGATCTCAATACGTGGGTAAGCGCTGCGCTGACCAATGAAGATACTTGCGTGGATGGCTTTCGAGGGGAAAGTGGAAAAGTATTGAATTTCCAAACTGGCTGCTTCCGGTTTCCAAACTGCCCCTAA

mRNA sequence

ATGTTGCGTGCACCCAGTTCGAGAGCATCAATTACAATCATTTTTGCCCTCATCTCCATTCTCCCATGGCCGACACACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGCGTAACACAACACCAAGATCTCTGTATCCAATTACTGTCGCCATTTTCAAGCAGCGCCAAGCGAAGCCCCACGAAATGGGCGCGAACGGGCGTGTCGGTGACAATATCAGAGGCTAAAACCGTGGCGCGATTCCTGGGGAGGGTGAAGAACAACAAGCGGCTCATGGGGAGAAACAGAGCCGCAGTTTCCGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTCCACAGGTCGCTTGGGGTTCTTAGAAGACTGAGTAAGAGGAATTTTGACGCCCAAATGGGGGATCTCAATACGTGGGTAAGCGCTGCGCTGACCAATGAAGATACTTGCGTGGATGGCTTTCGAGGGGAAAGTGGAAAAGTATTGAATTTCCAAACTGGCTGCTTCCGGTTTCCAAACTGCCCCTAA

Coding sequence (CDS)

ATGTTGCGTGCACCCAGTTCGAGAGCATCAATTACAATCATTTTTGCCCTCATCTCCATTCTCCCATGGCCGACACACTCCGCCAAAACGAGCTATGTCCAAGAAGCCTGCAGCGTAACACAACACCAAGATCTCTGTATCCAATTACTGTCGCCATTTTCAAGCAGCGCCAAGCGAAGCCCCACGAAATGGGCGCGAACGGGCGTGTCGGTGACAATATCAGAGGCTAAAACCGTGGCGCGATTCCTGGGGAGGGTGAAGAACAACAAGCGGCTCATGGGGAGAAACAGAGCCGCAGTTTCCGACTGTGTGGAGGTCTTCGAAGCCGCCATTGATGAGCTCCACAGGTCGCTTGGGGTTCTTAGAAGACTGAGTAAGAGGAATTTTGACGCCCAAATGGGGGATCTCAATACGTGGGTAAGCGCTGCGCTGACCAATGAAGATACTTGCGTGGATGGCTTTCGAGGGGAAAGTGGAAAAGTATTGAATTTCCAAACTGGCTGCTTCCGGTTTCCAAACTGCCCCTAA

Protein sequence

MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP
Homology
BLAST of CmaCh17G010870 vs. ExPASy Swiss-Prot
Match: O22244 (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 5.3e-26
Identity = 59/132 (44.70%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 30  TSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLGRVKNN 89
           ++YV+ AC+VT++  LC++ L PF+  A+ + +KWAR  V+VTI++ K V R L  +K  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  KRLMGRN-RAAVSDCVEVFEAAIDELHRSLGVLRRLSKRNFDAQMGDLNTWVSAALTNED 149
           +  +G + R A+SDC E+F  ++D L++SL VLR L+   F  Q+ DL TW+SAALT++D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFRGESGK 161
           TC+DGF   S +
Sbjct: 166 TCLDGFEETSSR 175

BLAST of CmaCh17G010870 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 51/156 (32.69%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 12  TIIF-ALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVS 71
           T++F AL+ IL   + +    +++ +C++T +  +C Q LS ++ + + +P + A T + 
Sbjct: 7   TLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQ 66

Query: 72  VTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSK---R 131
           V+++  +    F+ R+   K L  R  AA+ DC+E  E ++D + RS   ++ LS     
Sbjct: 67  VSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGN 126

Query: 132 NFDAQMGDLNTWVSAALTNEDTCVDGF--RGESGKV 162
           +F  +M ++ TWVSAALT+E TC+DGF  +G  GK+
Sbjct: 127 DFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKI 162

BLAST of CmaCh17G010870 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 2.4e-18
Identity = 47/135 (34.81%), Postives = 85/135 (62.96%), Query Frame = 0

Query: 25  THSAKTS--YVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARF 84
           T+S +T+  Y++ +C++T ++ +C   LSP++S+ + +P K A   +++T+S AK+ ++F
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 85  LGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSKRN---FDAQMGDLNTW 144
           +  + +   L      AV+DCVE    ++  L  S+  L  ++ ++   F+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 145 VSAALTNEDTCVDGF 155
           VSAALTN+DTC+DGF
Sbjct: 258 VSAALTNDDTCMDGF 272

BLAST of CmaCh17G010870 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.1e-18
Identity = 47/136 (34.56%), Postives = 79/136 (58.09%), Query Frame = 0

Query: 26  HSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLGR 85
           H+     V  +C    +  LC++ LS +S     +    A+  + +++S A++ A+ L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 86  VKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRL-----SKRNFDAQMGDLNTWV 145
           V+++     + +AA+ DCVE+   ++DEL R+LGVL+ L     S + F  QM +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 146 SAALTNEDTCVDGFRG 157
           SAALT++DTC+DGF+G
Sbjct: 147 SAALTDDDTCLDGFQG 162

BLAST of CmaCh17G010870 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.5e-15
Identity = 46/145 (31.72%), Postives = 77/145 (53.10%), Query Frame = 0

Query: 25  THSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLG 84
           T     +++Q +C  T +  +C+  L+ +++S + SP + A T ++VT+++A++   F+ 
Sbjct: 31  TSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFVW 90

Query: 85  RVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRL----SKRNFDAQMGDLNTWV 144
           R+     L  R   AV DC+E    A+D L  S+  ++       +  F   M +  TW 
Sbjct: 91  RLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTWT 150

Query: 145 SAALTNEDTCVDGFRGE--SGKVLN 164
           SAALTN +TC DGF G    G+V N
Sbjct: 151 SAALTNANTCSDGFAGRVMDGRVKN 175

BLAST of CmaCh17G010870 vs. ExPASy TrEMBL
Match: A0A6J1JRQ0 (pectinesterase inhibitor 6-like OS=Cucurbita maxima OX=3661 GN=LOC111488304 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.1e-93
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS
Sbjct: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP 176
           LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP
Sbjct: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP 175

BLAST of CmaCh17G010870 vs. ExPASy TrEMBL
Match: A0A6J1GQH3 (pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111456166 PE=4 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 2.6e-76
Identity = 150/163 (92.02%), Postives = 153/163 (93.87%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPSSRASI IIFALISILPWPTHSAK SYVQEACSVTQHQDLCIQ LSPFSSSAKRS
Sbjct: 1   MLRAPSSRASIAIIFALISILPWPTHSAKMSYVQEACSVTQHQDLCIQSLSPFSSSAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWA+ GVSVTISEAKTVARFLGRVKN KRLMGRNRAAVSDCVEV EAAI ELHRSLGV
Sbjct: 61  PTKWAQAGVSVTISEAKTVARFLGRVKNGKRLMGRNRAAVSDCVEVCEAAIAELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLN 164
           LRRL KRN DAQMGDL TWVSAALTNEDTCVDGFRG+SGKV+N
Sbjct: 121 LRRLGKRNLDAQMGDLTTWVSAALTNEDTCVDGFRGKSGKVVN 163

BLAST of CmaCh17G010870 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.1e-69
Identity = 136/162 (83.95%), Postives = 146/162 (90.12%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPS RASI  + ALISILPW THSAKTSYVQEAC VT+HQDLCIQ LSPFSS+AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR GVSVTI+EAK VA  LGR+KNNKR+ GRNRAAV DCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVL 163
           LRRLS+RNFDAQMGDL TWVSAALT+EDTCV+GF GE GKV+
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

BLAST of CmaCh17G010870 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.6e-68
Identity = 132/163 (80.98%), Postives = 146/163 (89.57%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           M+R PS+RASI  + ALISILPW THSAKTSYVQEAC VT HQDLCIQ LSPFSS+AKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR GVSVTI+EAK VA  LGR+KNNKR+ GRNRAAV DCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLN 164
           LRRLS+RNFD+QMGDL TWVSAALT+EDTCV+GF GE GKV++
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVS 163

BLAST of CmaCh17G010870 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 7.6e-68
Identity = 132/163 (80.98%), Postives = 146/163 (89.57%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           M+R PS+RASI  + ALISILPW THSAKTSYVQEAC VT HQDLCIQ LSPFSS+AKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR GVSVTI+EAK VA  LGR+KNNKR+ GRNRAAV DCVEVF+AAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLN 164
           LRRLS+RNFD+QMGDL TWVSAALT+EDTCV+GF GE GKV++
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVS 163

BLAST of CmaCh17G010870 vs. NCBI nr
Match: XP_022991771.1 (pectinesterase inhibitor 6-like [Cucurbita maxima])

HSP 1 Score: 352.4 bits (903), Expect = 2.2e-93
Identity = 175/175 (100.00%), Postives = 175/175 (100.00%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS
Sbjct: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP 176
           LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP
Sbjct: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLNFQTGCFRFPNCP 175

BLAST of CmaCh17G010870 vs. NCBI nr
Match: XP_023548990.1 (pectinesterase inhibitor 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 308.5 bits (789), Expect = 3.6e-80
Identity = 156/163 (95.71%), Postives = 158/163 (96.93%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPSSRASI IIFALISILPWPTHSAK SYVQEACSVTQHQDLCIQLL+PFSSSAKRS
Sbjct: 1   MLRAPSSRASIAIIFALISILPWPTHSAKKSYVQEACSVTQHQDLCIQLLTPFSSSAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR  VSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAAVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLN 164
           LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGF GESGKV+N
Sbjct: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFGGESGKVVN 163

BLAST of CmaCh17G010870 vs. NCBI nr
Match: XP_022953719.1 (pectinesterase inhibitor 6-like [Cucurbita moschata])

HSP 1 Score: 294.7 bits (753), Expect = 5.4e-76
Identity = 150/163 (92.02%), Postives = 153/163 (93.87%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPSSRASI IIFALISILPWPTHSAK SYVQEACSVTQHQDLCIQ LSPFSSSAKRS
Sbjct: 1   MLRAPSSRASIAIIFALISILPWPTHSAKMSYVQEACSVTQHQDLCIQSLSPFSSSAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWA+ GVSVTISEAKTVARFLGRVKN KRLMGRNRAAVSDCVEV EAAI ELHRSLGV
Sbjct: 61  PTKWAQAGVSVTISEAKTVARFLGRVKNGKRLMGRNRAAVSDCVEVCEAAIAELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVLN 164
           LRRL KRN DAQMGDL TWVSAALTNEDTCVDGFRG+SGKV+N
Sbjct: 121 LRRLGKRNLDAQMGDLTTWVSAALTNEDTCVDGFRGKSGKVVN 163

BLAST of CmaCh17G010870 vs. NCBI nr
Match: KAE8646342.1 (hypothetical protein Csa_016554 [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 6.4e-69
Identity = 136/162 (83.95%), Postives = 146/162 (90.12%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPS RASI  + ALISILPW THSAKTSYVQEAC VT+HQDLCIQ LSPFSS+AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR GVSVTI+EAK VA  LGR+KNNKR+ GRNRAAV DCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVL 163
           LRRLS+RNFDAQMGDL TWVSAALT+EDTCV+GF GE GKV+
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

BLAST of CmaCh17G010870 vs. NCBI nr
Match: XP_011659361.1 (pectinesterase inhibitor 6 [Cucumis sativus])

HSP 1 Score: 271.2 bits (692), Expect = 6.4e-69
Identity = 136/162 (83.95%), Postives = 146/162 (90.12%), Query Frame = 0

Query: 1   MLRAPSSRASITIIFALISILPWPTHSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRS 60
           MLRAPS RASI  + ALISILPW THSAKTSYVQEAC VT+HQDLCIQ LSPFSS+AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARTGVSVTISEAKTVARFLGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGV 120
           PTKWAR GVSVTI+EAK VA  LGR+KNNKR+ GRNRAAV DCVEVFEAAIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGESGKVL 163
           LRRLS+RNFDAQMGDL TWVSAALT+EDTCV+GF GE GKV+
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVV 162

BLAST of CmaCh17G010870 vs. TAIR 10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-33
Identity = 76/155 (49.03%), Postives = 105/155 (67.74%), Query Frame = 0

Query: 6   SSRASITIIFALISILPWPT-HSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKW 65
           SS  +  ++F L+S+ P  +  S + SYVQ ACSVT++QDLC + L PF+S AK SP+KW
Sbjct: 6   SSSITFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKNSPSKW 65

Query: 66  ARTGVSVTISEAKTVARFLGRVKNNKRLMG-RNRAAVSDCVEVFEAAIDELHRSLGVLRR 125
           AR GVSV I++ K V R L  +K     +G R+R A+SDC E+ + ++D LH+SL VLR 
Sbjct: 66  ARAGVSVAITDNKDVLRHL--LKTRLSTIGKRDRIALSDCRELLQDSLDSLHKSLAVLRT 125

Query: 126 LSKRNFDAQMGDLNTWVSAALTNEDTCVDGFRGES 159
           L    F  QM DL TW+S++LT++DTC+DGF   S
Sbjct: 126 LRASEFQQQMSDLATWLSSSLTDKDTCLDGFEKTS 158

BLAST of CmaCh17G010870 vs. TAIR 10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 3.8e-27
Identity = 59/132 (44.70%), Postives = 91/132 (68.94%), Query Frame = 0

Query: 30  TSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLGRVKNN 89
           ++YV+ AC+VT++  LC++ L PF+  A+ + +KWAR  V+VTI++ K V R L  +K  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  KRLMGRN-RAAVSDCVEVFEAAIDELHRSLGVLRRLSKRNFDAQMGDLNTWVSAALTNED 149
           +  +G + R A+SDC E+F  ++D L++SL VLR L+   F  Q+ DL TW+SAALT++D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFRGESGK 161
           TC+DGF   S +
Sbjct: 166 TCLDGFEETSSR 175

BLAST of CmaCh17G010870 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 4.0e-21
Identity = 51/139 (36.69%), Postives = 83/139 (59.71%), Query Frame = 0

Query: 25  THSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLG 84
           T +  T +V+ +C+ T +  LC   LS  +S  + SP   A   +++T++ AK  +  + 
Sbjct: 36  TTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMV 95

Query: 85  RVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSKRNFDAQMGDLNTWVSAAL 144
           R+ +N RL  +  +A+ DCVE     ++EL +S+G + +LS  N++  + D+ TWVSAAL
Sbjct: 96  RL-SNSRLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAAL 155

Query: 145 TNEDTCVDGFRGE--SGKV 162
           T+ +TC DGF GE   GKV
Sbjct: 156 TDVNTCTDGFEGEDMDGKV 173

BLAST of CmaCh17G010870 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 93.6 bits (231), Expect = 1.7e-19
Identity = 47/135 (34.81%), Postives = 85/135 (62.96%), Query Frame = 0

Query: 25  THSAKTS--YVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARF 84
           T+S +T+  Y++ +C++T ++ +C   LSP++S+ + +P K A   +++T+S AK+ ++F
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 85  LGRVKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRLSKRN---FDAQMGDLNTW 144
           +  + +   L      AV+DCVE    ++  L  S+  L  ++ ++   F+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 145 VSAALTNEDTCVDGF 155
           VSAALTN+DTC+DGF
Sbjct: 258 VSAALTNDDTCMDGF 272

BLAST of CmaCh17G010870 vs. TAIR 10
Match: AT5G20740.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.2e-19
Identity = 47/136 (34.56%), Postives = 79/136 (58.09%), Query Frame = 0

Query: 26  HSAKTSYVQEACSVTQHQDLCIQLLSPFSSSAKRSPTKWARTGVSVTISEAKTVARFLGR 85
           H+     V  +C    +  LC++ LS +S     +    A+  + +++S A++ A+ L  
Sbjct: 27  HNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTITNRRDLAQAAIKISLSHAQSAAKKLAV 86

Query: 86  VKNNKRLMGRNRAAVSDCVEVFEAAIDELHRSLGVLRRL-----SKRNFDAQMGDLNTWV 145
           V+++     + +AA+ DCVE+   ++DEL R+LGVL+ L     S + F  QM +  TW 
Sbjct: 87  VRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLGVLKHLRVSGGSAKEFRWQMSNAQTWA 146

Query: 146 SAALTNEDTCVDGFRG 157
           SAALT++DTC+DGF+G
Sbjct: 147 SAALTDDDTCLDGFQG 162

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222445.3e-2644.70Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1[more]
P174071.8e-1832.6921 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9SI742.4e-1834.81Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1[more]
Q84WE43.1e-1834.56Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1[more]
Q9STY51.5e-1531.72Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JRQ01.1e-93100.00pectinesterase inhibitor 6-like OS=Cucurbita maxima OX=3661 GN=LOC111488304 PE=4... [more]
A0A6J1GQH32.6e-7692.02pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111456166 PE... [more]
A0A0A0KAX03.1e-6983.95PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S... [more]
A0A5D3D1R17.6e-6880.9821 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630... [more]
A0A1S3BSW57.6e-6880.9821 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022991771.12.2e-93100.00pectinesterase inhibitor 6-like [Cucurbita maxima][more]
XP_023548990.13.6e-8095.71pectinesterase inhibitor 6-like [Cucurbita pepo subsp. pepo][more]
XP_022953719.15.4e-7692.02pectinesterase inhibitor 6-like [Cucurbita moschata][more]
KAE8646342.16.4e-6983.95hypothetical protein Csa_016554 [Cucumis sativus][more]
XP_011659361.16.4e-6983.95pectinesterase inhibitor 6 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G62820.11.2e-3349.03Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT2G47670.13.8e-2744.70Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G62360.14.0e-2136.69Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT1G62760.11.7e-1934.81Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G20740.12.2e-1934.56Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 28..169
e-value: 1.2E-31
score: 121.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 9..160
e-value: 5.2E-33
score: 111.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 32..157
e-value: 4.1E-30
score: 105.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..167
e-value: 4.3E-30
score: 106.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 31..159
NoneNo IPR availablePANTHERPTHR31080:SF158PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEINcoord: 20..160
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 20..160
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 36..161
e-value: 3.2518E-37
score: 124.088

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G010870.1CmaCh17G010870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity
molecular_function GO:0030599 pectinesterase activity