CmaCh17G008190 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh17G008190
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	thionin-like protein 2
Location	Cma_Chr17: 6436238 .. 6436825 (-)
RNA-Seq Expression	CmaCh17G008190
Synteny	CmaCh17G008190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTGTGAGCAAAATTTCCTGTACCCATTTCTTCGTTTCTTATTTTTCAAACATGTTTTCATTTTGTGTTATGAACTTGGCAGCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAGAAACATGAAGAAGGGAGGAGGAGCTGGGATTGGCCTTAACACCATCAATAAATAAACATATTTTGGTGTATGCATGGTTTCAAGTCTCTGCCCGTGTTATGTGAACCATATGCCTTAAATGTTTAAAAACTTATTAGACACAAAATTTGATCTCAAAGTTTGAGGGGCCAAGAGCTCTAAAC

mRNA sequence

ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAGAAACATGAAGAAGGGAGGAGGAGCTGGGATTGGCCTTAACACCATCAATAAATAAACATATTTTGGTGTATGCATGGTTTCAAGTCTCTGCCCGTGTTATGTGAACCATATGCCTTAAATGTTTAAAAACTTATTAGACACAAAATTTGATCTCAAAGTTTGAGGGGCCAAGAGCTCTAAAC

Coding sequence (CDS)

ATGAAGTCAATTGTTCTAATTTGCTTCATTCTGAGCTTAGTGGCAGGAAGGTCCACAGCGTCCTTTAGGAAGTGCTATGCCAAGTGCTTCGTAGTGTGTGCTATCACACCAGGCATCACCCTTGGCACCTGCGGTGCAACGTGCTTGGCGAAGTGCCTGTTCATTGCATCTGCCCCAATGGATTTCAACCATATGGACACACATTACTTCTGCAAACTTGGGTGTGCCACTTCCATGTGCACCAAATTCAGCACCAAGAACGACCCTTCTGAGAAGAAAGTGGAAAGGTGTGTGGACTCCTGCGCTGGAACATGCATCAAAGCTTAG

Protein sequence

MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPMDFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA

Homology

[Close]

BLAST of CmaCh17G008190 vs. ExPASy Swiss-Prot
Match: A8MRP4 (Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 1.6e-09
Identity = 39/109 (35.78%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 5   VLICFILSLVAGRSTAS---FRKCYAKCFVVC-AITPGITLGTCGATCLAKCLFIASAP- 64
           V+I  ++  +  ++ A    F++CY  C V C A +       C  TC  +CL   S P 
Sbjct: 5   VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 65  MDFNHMD-THYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  N++D + YFCKLGCAT  C   S+  +P+ ++V  CVDSC+  C K
Sbjct: 65  VSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

[Close]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL
Match: A0A6J1KDK0 (thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.5e-45
Identity = 86/108 (79.63%), Postives = 98/108 (90.74%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           MKS+V+ICFILSLVAGRSTASF KCYAKCF+VCAITPG+ +GTCG  CLA CLF+ASAP 
Sbjct: 36  MKSVVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFLASAPR 95

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           D NH+DTHYFCKLGCATS+CTKFSTK DP+EKKVE CV+SC+ TC+KA
Sbjct: 96  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 143

[Close]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL
Match: A0A0A0KC18 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 2.3e-38
Identity = 78/92 (84.78%), Postives = 84/92 (91.30%), Query Frame = 0


Query: 1  MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
          MKS+VLICFILSLVAGRSTASF KCYAKCF+VCAITPGI +GTCGA CLA CLFIAS+PM
Sbjct: 1  MKSVVLICFILSLVAGRSTASFGKCYAKCFIVCAITPGIPVGTCGAKCLADCLFIASSPM 60

Query: 61 DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEK 93
          D N+MDTHYFCKLGCATS CTKFSTK DP +K
Sbjct: 61 DLNYMDTHYFCKLGCATSRCTKFSTKKDPGKK 92

[Close]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL
Match: A0A6J1DDL8 (thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 7.6e-34
Identity = 75/107 (70.09%), Postives = 85/107 (79.44%), Query Frame = 0


Query: 4   IVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLF-IASAPMDF 63
           ++L+  +LSLVAG+STASF KCYAKCFVVCAITPGI +GTC A CL  CLF  AS   DF
Sbjct: 6   LILVMLVLSLVAGKSTASFGKCYAKCFVVCAITPGIPVGTCAAKCLTDCLFRAASTTADF 65

Query: 64  N-HMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           N  +DT YFCKLGCATS+CTKFSTK DP+EKKV  CVDSC+  CI A
Sbjct: 66  NDQIDTRYFCKLGCATSLCTKFSTKKDPAEKKVGSCVDSCSQKCINA 112

[Close]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL
Match: A0A5D3BCH5 (Thionin-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold85G00550 PE=4 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.2e-24
Identity = 64/111 (57.66%), Postives = 74/111 (66.67%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRST--ASFRKCYAKCFVVCAITPGI-TLGTCGATCLAKCLFIAS 60
           M+SIVLI FILSLVAGRST      K + KC   CAI P I  +  C   C+A C+ +AS
Sbjct: 1   MRSIVLIHFILSLVAGRSTPVLDTAKWFVKCMAECAILPIIPVVSICAVKCMADCIPVAS 60

Query: 61  APMDFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           +PMD N MDTHYFCKLGC T  CTK STK D  E KVE C ++C  TC+KA
Sbjct: 61  SPMDLNRMDTHYFCKLGCTTYRCTKLSTKKDLVENKVETCGNTCDQTCMKA 111

[Close]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL
Match: A0A1Q3BXG2 (Uncharacterized protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_16158 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.3e-24
Identity = 52/107 (48.60%), Postives = 75/107 (70.09%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           +KS++++C +L L+ G+STASF++CY  CF+ C I    ++ +C A CL  C+F +S+ +
Sbjct: 6   VKSLLMVCLVLGLLLGQSTASFKRCYGNCFITCVIESDSSIISCAAKCLKHCIFHSSSAI 65

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
                 T Y+CKLGCATS+CT  STK DP E+KVE CVDSC+  CI+
Sbjct: 66  S---SSTQYYCKLGCATSLCTHISTKQDPGEEKVEACVDSCSNKCIQ 109

[Close]

BLAST of CmaCh17G008190 vs. NCBI nr
Match: KAG7014098.1 (Thionin-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 220.3 bits (560), Expect = 8.0e-54
Identity = 102/108 (94.44%), Postives = 107/108 (99.07%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           MKS+V+ICFILSL+AGRSTASFRKCYAKCF+ CAITPGITLGTCGATCLAKCLFIASAPM
Sbjct: 1   MKSVVIICFILSLMAGRSTASFRKCYAKCFIACAITPGITLGTCGATCLAKCLFIASAPM 60

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           DFNHM+THYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA
Sbjct: 61  DFNHMNTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 108

[Close]

BLAST of CmaCh17G008190 vs. NCBI nr
Match: KAG6575552.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 200.3 bits (508), Expect = 8.5e-48
Identity = 91/93 (97.85%), Postives = 92/93 (98.92%), Query Frame = 0


Query: 16  GRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPMDFNHMDTHYFCKLGC 75
           GRSTASFRKCYAKCF+ CAITPGITLGTCGATCLAKCLFIASAPMDFNHMDTHYFCKLGC
Sbjct: 84  GRSTASFRKCYAKCFIACAITPGITLGTCGATCLAKCLFIASAPMDFNHMDTHYFCKLGC 143

Query: 76  ATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           ATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA
Sbjct: 144 ATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 176

[Close]

BLAST of CmaCh17G008190 vs. NCBI nr
Match: XP_038899787.1 (thionin-like protein 2 [Benincasa hispida])

HSP 1 Score: 194.5 bits (493), Expect = 4.7e-46
Identity = 88/108 (81.48%), Postives = 98/108 (90.74%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           MKS++LICFI+SLV GRSTASF KCYAKCFVVCAITPGI +GTCGA CL  CLFIAS+P+
Sbjct: 1   MKSVILICFIMSLVVGRSTASFGKCYAKCFVVCAITPGIPIGTCGAKCLGDCLFIASSPL 60

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           DFNH+DTHYFCKLGCATS+CTKFSTK DP+EKKVE CV+SC  TCIKA
Sbjct: 61  DFNHLDTHYFCKLGCATSLCTKFSTKKDPAEKKVESCVNSCGQTCIKA 108

[Close]

BLAST of CmaCh17G008190 vs. NCBI nr
Match: XP_023000407.1 (thionin-like protein 2 [Cucurbita maxima])

HSP 1 Score: 191.8 bits (486), Expect = 3.0e-45
Identity = 86/108 (79.63%), Postives = 98/108 (90.74%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           MKS+V+ICFILSLVAGRSTASF KCYAKCF+VCAITPG+ +GTCG  CLA CLF+ASAP 
Sbjct: 36  MKSVVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFLASAPR 95

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           D NH+DTHYFCKLGCATS+CTKFSTK DP+EKKVE CV+SC+ TC+KA
Sbjct: 96  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 143

[Close]

BLAST of CmaCh17G008190 vs. NCBI nr
Match: KAG6593441.1 (Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 190.3 bits (482), Expect = 8.8e-45
Identity = 87/108 (80.56%), Postives = 97/108 (89.81%), Query Frame = 0


Query: 1   MKSIVLICFILSLVAGRSTASFRKCYAKCFVVCAITPGITLGTCGATCLAKCLFIASAPM 60
           MKSIV+ICFILSLVAGRSTASF KCYAKCF+VCAITPG+ +GTCG  CLA CLF ASAP 
Sbjct: 1   MKSIVVICFILSLVAGRSTASFGKCYAKCFIVCAITPGVPIGTCGGKCLADCLFPASAPR 60

Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIKA 109
           D NH+DTHYFCKLGCATS+CTKFSTK DP+EKKVE CV+SC+ TC+KA
Sbjct: 61  DLNHLDTHYFCKLGCATSLCTKFSTKTDPAEKKVESCVNSCSRTCLKA 108

[Close]

BLAST of CmaCh17G008190 vs. TAIR 10
Match: AT1G12663.1 (Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant thionin (PR-13) family with the following members: At1g66100, At5g36910, At1g72260, At2g15010, At1g12663, At1g12660. )

HSP 1 Score: 63.5 bits (153), Expect = 1.2e-10
Identity = 39/109 (35.78%), Postives = 59/109 (54.13%), Query Frame = 0


Query: 5   VLICFILSLVAGRSTAS---FRKCYAKCFVVC-AITPGITLGTCGATCLAKCLFIASAP- 64
           V+I  ++  +  ++ A    F++CY  C V C A +       C  TC  +CL   S P 
Sbjct: 5   VMIVMVIGNLLAQTAAQKIPFKECYPACLVECKAGSKFPKYLKCPFTCTKECLQQPSPPS 64

Query: 65  MDFNHMD-THYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTCIK 108
           +  N++D + YFCKLGCAT  C   S+  +P+ ++V  CVDSC+  C K
Sbjct: 65  VSSNNIDESDYFCKLGCATYHCVSLSSIQNPNVERVSACVDSCSNKCTK 113

[Close]

BLAST of CmaCh17G008190 vs. TAIR 10
Match: AT1G12672.2 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1). )

HSP 1 Score: 53.5 bits (127), Expect = 1.2e-07
Identity = 27/63 (42.86%), Postives = 36/63 (57.14%), Query Frame = 0


Query: 47  TCLAKCLFIASAPMD----FNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCA 106
           +C+  CL   S+ MD     NH D  Y+CKLGC+T  C   S+  +P+  KV  CVDSC+
Sbjct: 40  SCIKTCLEPPSSQMDSTNEINHND--YYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCS 99

[Close]

BLAST of CmaCh17G008190 vs. TAIR 10
Match: AT1G12672.1 (unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G12663.1); Has 4 Blast hits to 4 proteins in 1 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 4; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 48.9 bits (115), Expect = 3.0e-06
Identity = 21/45 (46.67%), Postives = 28/45 (62.22%), Query Frame = 0


Query: 61  DFNHMDTHYFCKLGCATSMCTKFSTKNDPSEKKVERCVDSCAGTC 106
           + NH D  Y+CKLGC+T  C   S+  +P+  KV  CVDSC+  C
Sbjct: 20  EINHND--YYCKLGCSTHHCASLSSIQNPNVDKVVDCVDSCSDKC 62

The following BLAST results are available for this feature:

BLAST of CmaCh17G008190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A8MRP4	1.6e-09	35.78	Thionin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=At1g12663 PE=3 SV=1	[more]

BLAST of CmaCh17G008190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KDK0	1.5e-45	79.63	thionin-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111494667 PE=4 SV=1	[more]
A0A0A0KC18	2.3e-38	84.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G446640 PE=4 SV=1	[more]
A0A6J1DDL8	7.6e-34	70.09	thionin-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111019402 PE=4 SV=1	[more]
A0A5D3BCH5	4.2e-24	57.66	Thionin-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold8...	[more]
A0A1Q3BXG2	9.3e-24	48.60	Uncharacterized protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_16158 PE=4...	[more]

BLAST of CmaCh17G008190 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014098.1	8.0e-54	94.44	Thionin-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6575552.1	8.5e-48	97.85	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038899787.1	4.7e-46	81.48	thionin-like protein 2 [Benincasa hispida]	[more]
XP_023000407.1	3.0e-45	79.63	thionin-like protein 2 [Cucurbita maxima]	[more]
KAG6593441.1	8.8e-45	80.56	Thionin-like protein 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh17G008190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12663.1	1.2e-10	35.78	Predicted to encode a PR (pathogenesis-related) protein. Belongs to the plant t...	[more]
AT1G12672.2	1.2e-07	42.86	unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana prot...	[more]
AT1G12672.1	3.0e-06	46.67	unknown protein; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana prot...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR36312:SF1	THIONIN-LIKE PROTEIN 1	coord: 2..107
IPR038975	Thionin-like protein	PANTHER	PTHR36312	THIONIN-LIKE PROTEIN 1	coord: 2..107

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G008190.1	CmaCh17G008190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane