CmaCh17G005240 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G005240
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionbeta-fructofuranosidase, insoluble isoenzyme 1-like
LocationCma_Chr17: 3608959 .. 3613218 (-)
RNA-Seq ExpressionCmaCh17G005240
SyntenyCmaCh17G005240
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCGTTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCACTGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCATCACTGGATGAACGGTTCGTAATTTCTACATTTTCATTCCCTCCTTTCCTCCGGTTGTTAACAATGTATTTCCTCCGGTTCATTCCCTCCATAAACATGTCATCTTATGCAGGCCCGAATGGCCCAATGTACTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCGGTTTGGGGGAATATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAATCGGATAACAATCCGATTGTTGACCCGGGTACGGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCGTGGCTGACCCTCAACGGTCACTGGAACACCATCATTGGCAGTAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGGATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTACCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGCTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGCAATTTCTATGCTTCCAAGTCGTTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGTTTGTTTTAGATCCAATGTGCTCCGTTTTGTTCTTTTTTTAATTGAATTTGTTCTATTTTGTATTTGGGTCAACCTTTTCTTTCTCCTTTTCCTAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAGCAGCTCTTACAATGGCCAGTTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGTTAAATCAAAAGCTAGTCAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGTTGTTTAATTTATAACGAATCCGATCTTAACCCAAATCCCCGACAGTGGTTTGTAGCGGGTTAAGAAGATGTGAAAGCACCTCTAAGATTTAAGGGAACAAACTGGGTGTCATTTGATCGATTGTTTCTTGTGTTTTATACAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGTTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTCGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGGTAGCTTCAATTCCCTTATCAAAGGACCTTATAATTATTTTTTTATATTTACAAAGCTCTGGTTTGTCTCTTTTGGTGTAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCAAGTAAAAAGCTCTCATTAAGAAGCTTGGTACGTTAAAGTCATAGTCCAATACATTCTCGCACCGTATCGACAAATCTAAACTATGTATTATTGTGCAGATTGATCACTCGGTGATAGAGAGCTTTGGTGGAGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTCGGGTGAACTAAGTCTTTTTTAAGTTGGAACAGCTTTCTAACCGCTAATAATGTTAGAACTAACTCTTAGTTTTTGATTAATTAAGGTTGTAATGGTAGATTAGCCTCCTTTTTTGTCTATGAATAAAGAGTTAGATTTTCAGAATTTCCCTTTGTTGATCTTTATTAATTTATAAATACTTTTAGTATTATAACAATTAATTCTATAGACAACCCTTGACACCCAGTATGAAAATAAATAAAGTATAAACAGCTAAAAATATACTTTTATAAATTCAAATTAAAAAAATTAATAAAGAAATAATTCCTTTTTGAATAAGGTTCTTGCATTATTGTGGAGCAAGTACATTTTATAAAGTTATTCTCATATTTCAAGTTATAAGATCTTCATTTATTTCTTACATTCATATATTAATTTTTTTAAATAATAAACCATTAACCCTAATGTTTTCTTCCTTGATGCTTTAAAGTTTTGAAATACCTTCCTCCTCTTCAATAAGAAAATCATGTAACACGAGTTACATTTCTACTAGTTAGAAGTGAACTCGAGTAGGGTTGAAAAAATCTTTTGGACCAACCTAAAATTTCAGGTTGATTGAATGTTAGGCAATGTGAGATAGTCAACAATTGTTCAATTTGAACCGGGCCTATAATATGGCTAGGCTAGACTCTTCCTAGAAAAAAAGACTTAGACTATGGGTAGTTGGTGATTCCATATATATGGGTAGTAAGAGGGCGCTTATGCCGAGACCCGTATGGGGAAGGGCCCAACCTAATAGGGACAGCACATCCCCACACTATTTACTTGGTAGTTGGTGATTCGATATATATGGGTAGCAAGAGGGCGCTTATGCCCCGATGGTGGGGCCTTATGGGGTAGGGCCAACCCCAATAGGGCCATCACACCCCCACTTTAAGCGCACCTCTGTCCCCGATGGTGGGCCTTATGGGGAAGGTGGGGAAGGGCTCACCCCAATAGGGACAGCACACCCCCACTTTAAGCGCACCTCTGTCCCCGATGGTGGGCCTTATGGGGAAGGTGGGGAAGGGCTCACCCCAATACTTTAAGCGCACCTCTGTCCCCGATGGTGGGCCTTATGGGGAAGGTGGGGAAGGGCTCACCCCAATAGTGACAGCACACCCCCACTTTAAGCGCATTGTATCACTTTTTCACTCTTTCTAGTTGGTCGAATCAGTGAACCACGCGAGCAGGTTAGATGTGTGGGGCAGAGGGTTCGTAGTACCCCCTTTTCTTGATCTATCCTTTTCTTCCCCCTTTTCTTCGCTGAATCACCTACTAAAAGGCAAGCCTGCACGCTGAGACTGACTACTAAGGACTACTAAGGTAGGCGGTGGACTCTTTCATTGGGGAAGGGAAGAAGGGGCCTAAGCACGACAGATGCTGTACATACACTTGAGTGGCAAAGGAAAGCGAGATCGTACCTCTTTTTCCAGGCCTGTTCGGACATACGGTTCCCGCGAAAGATCAAGTTGGTGAGCCGTGTGAATGGAAAACCTTCCGCACGGTTCAGAGAGCACTGAACTAGAATGAGAGGTTCATCACCACATCATTGCATGCAAGAGGAGCTAGCTCGATTTGCAAATCAATCCGAAATTCACTTTTGACTCTGTGTTCGATAGCCTGACCATAGTGATGCTAATTGTGGTTACATTCATAAGTAGCTTGGTCCATCTTTATTCCATTTCATATATGTCTGAGGATCCGCATAGCACTCGATTTATGTGTTATTTATCCATTTTTACTTTTTTTATGCCAATGTTGGTGACTGGAGATAAGTCTCTTCAATTATTTATAGGATGAGAAGGAGTAGGTCTTGCTTCATATTTGTTAATTCATTTCTGGTTTATACGATTTCAGGCAGATAAAGCAGCTATAAAAGCTATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCTTGGGATTTCGGGTCGTTTTACTCTATTTCAAACAGTAGACTTTTCAACTATTTTTGCTCATGCTGGTGCCCCAGAAATTCTTGGATTTCTTGCAATATGA

mRNA sequence

ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCGTTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCACTGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCATCACTGGATGAACGGCCCGAATGGCCCAATGTACTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCGGTTTGGGGGAATATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAATCGGATAACAATCCGATTGTTGACCCGGGTACGGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCGTGGCTGACCCTCAACGGTCACTGGAACACCATCATTGGCAGTAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGGATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTACCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGCTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGCAATTTCTATGCTTCCAAGTCGTTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAGCAGCTCTTACAATGGCCAGTTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGTTAAATCAAAAGCTAGTCAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGTTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTCGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCAAGTAAAAAGCTCTCATTAAGAAGCTTGATTGATCACTCGGTGATAGAGAGCTTTGGTGGAGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTCGGGCAGATAAAGCAGCTATAAAAGCTATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCTTGGGATTTCGGGTCGTTTTACTCTATTTCAAACAGTAGACTTTTCAACTATTTTTGCTCATGCTGGTGCCCCAGAAATTCTTGGATTTCTTGCAATATGA

Coding sequence (CDS)

ATGAATTTCTTCACTCTCCTAGCTATTCTTTTCGGATTCTCCTTTTGTTCTCTGTTTATTGGCAATGGCCCTGTCGTCGTTCAAGCTCTCCATAAAATCTATCCTGAATTACAGTCTCTCGAAAATGGCAACGACACCACCACTGAGATCAAGCAGCTCCATCGAACCGGCTTCCACTTTCAACCTCATCATCACTGGATGAACGGCCCGAATGGCCCAATGTACTTCAAGGGAATCTACCATCTCTTCTACCAATACAACCCTGAAGGCGCGGTTTGGGGGAATATTGTCTGGGCTCACTCAATTTCCAAAGACCTTATCAATTGGAAGTCACTCAAACCCGCGCTTTCCCCCTCAAAGCCCTTTGATATCAATGGCTGCTGGTCTGGCTCCGCCACCGTCCTCCCCGGCCTCAAGCCCGTCATTTTGTACACTGGAATCGACACCCAGAATCGACAGGTACAAAACTACGCCATTCCAGCGAACCTCTCTGATCCGCATCTAACCGAGTGGGTCAAATCGGATAACAATCCGATTGTTGACCCGGGTACGGAGGTCAATAGTAGCGCCTTTCGGGACCCGTCGACGGCGTGGCTGACCCTCAACGGTCACTGGAACACCATCATTGGCAGTAGGAGCGAGCAGACGGGAATGGCGTATTTGTATAGAAGTAGGGATTTTTTGAATTGGACTAAGGTGGAACAGCCTTTACATTCGGTTCCGAATACTGGAATGTGGGAGTGCCCTGATTTCTACCCAGTTTCCCACTTCGGTAGATTTGGCTTGGACACGTCGGTGATGGGAAATTGGTTTAAACATGTGTTGAAGGTGAGCTTGGAGATGACTAGATATGAGTATTATACGCTGGGGAAGTATTATCCGATGACGGACAAGTATGTTCCCGATAATACCTCGGCGGATGGGTGGAGTGGGCTGAGGTATGACTATGGCAATTTCTATGCTTCCAAGTCGTTCTATGATCCGATGAAGAAGAGAAGGGTTTTGTGGGGTTGGGCTAATGAATCTGATAGTGTCCAGGATGATGTTTCCAAGGGATGGGCGGGAATTCAGGCGATTCCTAGGACGGTGTGGTTGGATCCCAATGGAAAGCAGCTCTTACAATGGCCAGTTATGGAGCTCAATAGGCTGAGACGGAAGATAGTTATGATGTTAAATCAAAAGCTAGTCAAGGGACAAAATGTTGAGATCAAAGGAATTACTGCGGCTCAGGCCGATGTTGAAGTTATCTTTAGCTTTTCGAGTTTGGATAACGCGGAGGCATTTGATCCAAGTTGGGTTGACGCGCAAGCCGTGTGCAGTCAGCTGGGATCCACGGCCCAAGGTGGTGTCGGACCCTTTGGGCTTTTGACTCTGGCTTCAGAAAACCTCGAGGAGTTCACTCCTGTGTTCTTCAGGATCTTCAAGGCAGAGGCTAATCACGTGGTTCTTATGTGCTCTGATGCAAGAAGCTCATCTTTGAAAGATGGATTATACAAGCCGACATTTGCGGGTTTCGTCGACATCGATTTGTCAAGTAAAAAGCTCTCATTAAGAAGCTTGATTGATCACTCGGTGATAGAGAGCTTTGGTGGAGGAGGAAAAACATGCATAACTTCGAGGGTATACCCAACAAAGGCGGTGTTTGGGGATGCTCATTTGCATGTTTTCAACAATGGAACAGAGTCTATCACGGTGGAGTATTTAAGTGCTTGGAGTATGAGAAGTGCTCGGGCAGATAAAGCAGCTATAAAAGCTATGCCTGTCAATCGAGTAGGTGATTTTGGATTAGCTCTTGGGATTTCGGGTCGTTTTACTCTATTTCAAACAGTAGACTTTTCAACTATTTTTGCTCATGCTGGTGCCCCAGAAATTCTTGGATTTCTTGCAATATGA

Protein sequence

MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARADKAAIKAMPVNRVGDFGLALGISGRFTLFQTVDFSTIFAHAGAPEILGFLAI
Homology
BLAST of CmaCh17G005240 vs. ExPASy Swiss-Prot
Match: P26792 (Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 PE=1 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 6.6e-228
Identity = 374/555 (67.39%), Postives = 447/555 (80.54%), Query Frame = 0

Query: 27  VQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQY 86
           V+A H+I+  LQS+   N     +KQ+HRTG+HFQP  +W+N PNGPMY+KG+YHLFYQY
Sbjct: 37  VEAFHEIHYNLQSVGAEN-----VKQVHRTGYHFQPKQNWINDPNGPMYYKGVYHLFYQY 96

Query: 87  NPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTG 146
           NP+GAVWGNIVWAHS+S DLINW  L+PA+ PSKPFD  GC SGSAT+LPG KPVILYTG
Sbjct: 97  NPKGAVWGNIVWAHSVSTDLINWTPLEPAIFPSKPFDKYGCRSGSATILPGNKPVILYTG 156

Query: 147 I---DTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLTLNG 206
           I     +N QVQNYAIPANLSDP+L +W+K DNNP+V      N++AFRDP+TAWL  +G
Sbjct: 157 IVEGPPKNVQVQNYAIPANLSDPYLRKWIKPDNNPLVVANNGENATAFRDPTTAWLDKSG 216

Query: 207 HWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFGRFGLD 266
           HW  ++GS+  + G+AYLYRS+DF+ WTK + P+HS  NTGMWECPDF+PVS  G  GLD
Sbjct: 217 HWKMLVGSKRNRRGIAYLYRSKDFIKWTKAKHPIHSQANTGMWECPDFFPVSLKGLNGLD 276

Query: 267 TSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASK 326
           TSV G   KHVLKVSL++TRYEYYT+G Y    D+Y+PDNTS DGW+GLRYDYGNFYASK
Sbjct: 277 TSVTGESVKHVLKVSLDLTRYEYYTVGTYLTDKDRYIPDNTSVDGWAGLRYDYGNFYASK 336

Query: 327 SFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRL 386
           +F+DP K RR+LWGWANESDS   DV+KGWAGIQ IPRT+WLDP+GKQL+QWP+ EL  L
Sbjct: 337 TFFDPSKNRRILWGWANESDSTAHDVAKGWAGIQLIPRTLWLDPSGKQLMQWPIEELETL 396

Query: 387 R-RKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV--DAQAVCS 446
           R  K+     Q L KG  VE+KGITAAQADVEV FSF SL   E FDP W+  DA+ +CS
Sbjct: 397 RGSKVKFSRKQDLSKGILVEVKGITAAQADVEVTFSFKSLAKREPFDPKWLEYDAEKICS 456

Query: 447 QLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHVVLMCSDARSSSLKDGLYKP 506
             GST QGGVGPFGLLTLASE LEE+TPVFFR+FKA+  H VLMCSDA  SSLK+GLY+P
Sbjct: 457 LKGSTVQGGVGPFGLLTLASEKLEEYTPVFFRVFKAQNTHKVLMCSDATRSSLKEGLYRP 516

Query: 507 TFAGFVDIDLSS-KKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLHVFNNG 566
           +FAGFVD+DL++ KK+SLRSLID+SV+ESFG  GKTCI+SRVYPT AV+ +AHL+VFNNG
Sbjct: 517 SFAGFVDVDLATDKKISLRSLIDNSVVESFGAKGKTCISSRVYPTLAVYENAHLYVFNNG 576

Query: 567 TESITVEYLSAWSMR 575
           +E+ITVE L AWSM+
Sbjct: 577 SETITVENLDAWSMK 586

BLAST of CmaCh17G005240 vs. ExPASy Swiss-Prot
Match: Q39693 (Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 PE=3 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 9.0e-225
Identity = 378/575 (65.74%), Postives = 448/575 (77.91%), Query Frame = 0

Query: 7   LAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHW 66
           L +L  FSF  L I NG   V + H+++PELQS+     +  ++K +HRTG+HFQP  HW
Sbjct: 15  LFLLSIFSFIFLNI-NG---VDSTHRVFPELQSI-----SAVDVKLVHRTGYHFQPQKHW 74

Query: 67  MNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDING 126
           +N PNGPM++KG YHLFYQYNP+G+VWGNIVWAHS+SKDLINW +L+PA+ PSKPFD  G
Sbjct: 75  INDPNGPMFYKGYYHLFYQYNPKGSVWGNIVWAHSVSKDLINWIALEPAIFPSKPFDQYG 134

Query: 127 CWSGSATVLPGLKPVILYTGI---DTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPG 186
           CWSGSAT+LPG KPVILYTGI   D +N QVQNYA+PAN SDP L EWVK DNNP+V   
Sbjct: 135 CWSGSATILPGNKPVILYTGIVSPDPENAQVQNYAVPANYSDPFLREWVKPDNNPLVGVH 194

Query: 187 TEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNT 246
           TE N SAFRDP+TAW    GHW  ++GS  +  G+AYLYRS+DF  W +   P+H+   T
Sbjct: 195 TE-NPSAFRDPTTAWFD-GGHWKMLVGSSRKHRGIAYLYRSKDFKKWKRSPHPIHTKAET 254

Query: 247 GMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDN 306
           GMWECPDFYPVS     GLD S MG   KHVLKVSL  TRYEYYT+G+Y  + D YVPDN
Sbjct: 255 GMWECPDFYPVSPRSEDGLDNSKMGRGIKHVLKVSLNSTRYEYYTIGRYNRVRDFYVPDN 314

Query: 307 TSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTV 366
           TS DGW+GLRYDYGNFYASK+FYDP+KKRR+LWGWANESDS  DDV KGWAGIQ IPR +
Sbjct: 315 TSVDGWAGLRYDYGNFYASKTFYDPIKKRRILWGWANESDSQIDDVQKGWAGIQLIPRRI 374

Query: 367 WLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLD 426
           WLDP+G+QL+QWP+ E+  LR   + M NQKL  G +VE+ GITAAQADV+  FSF SLD
Sbjct: 375 WLDPSGRQLVQWPIEEVEGLRGSELHMRNQKLDMGVHVEVTGITAAQADVDATFSFKSLD 434

Query: 427 NAEAFDPSWV--DAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANHV 486
            AE+FDP W+  DAQ VC  +GST QGG+GPFGLLTLAS++LEE+TPVFFRIFKAE   +
Sbjct: 435 KAESFDPEWINLDAQDVCDSMGSTIQGGLGPFGLLTLASKDLEEYTPVFFRIFKAEDQKL 494

Query: 487 -VLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 546
            VLMCSDA+ SSL +GLYKP+F GFVD+DLS KK+SLRSLID+SV+ESFG   K  I+SR
Sbjct: 495 KVLMCSDAKRSSLAEGLYKPSFRGFVDVDLSDKKISLRSLIDNSVVESFGAQRKNLISSR 554

Query: 547 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRS 576
           VYPT A++ +AHL VFNNGTE ITV+ L AWSM S
Sbjct: 555 VYPTLAIYNNAHLFVFNNGTEPITVDNLDAWSMNS 578

BLAST of CmaCh17G005240 vs. ExPASy Swiss-Prot
Match: Q39692 (Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 2.4e-222
Identity = 374/576 (64.93%), Postives = 451/576 (78.30%), Query Frame = 0

Query: 6   LLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHH 65
           L A++F FS      G     V+A H+++PELQS+     +   ++ +HRTG+HFQP  H
Sbjct: 22  LSAVVFIFSLDVNIRG-----VEASHQVFPELQSV-----SAVNVQLVHRTGYHFQPKKH 81

Query: 66  WMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDIN 125
           W+N PNGPMY+KG YHLFYQYNP+GAVWGNIVWAHSISKDLINW +L+PA+ PSKPFD  
Sbjct: 82  WINDPNGPMYYKGFYHLFYQYNPKGAVWGNIVWAHSISKDLINWVALEPAIFPSKPFDKY 141

Query: 126 GCWSGSATVLPGLKPVILYTGIDTQ---NRQVQNYAIPANLSDPHLTEWVKSDNNPIVDP 185
           GCWSGSATVLPG KPVI+YTGI T    N QVQN+A+PAN SDP+L EW+K DNNPIV  
Sbjct: 142 GCWSGSATVLPGGKPVIMYTGIVTPSPVNTQVQNFAVPANYSDPYLREWIKPDNNPIVRA 201

Query: 186 GTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPN 245
            +E NSS+FRDP+TAW     HW  ++GSR +  G+AYLYRSR+FL WTK + PLHS   
Sbjct: 202 RSE-NSSSFRDPTTAWFD-GVHWKILVGSRRKHRGIAYLYRSRNFLKWTKAKHPLHSKDR 261

Query: 246 TGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPD 305
           TGMWEC DFYPV+  G  GLDTSV G   KHVLKVSL  TRYEYYT+G+Y    D YVPD
Sbjct: 262 TGMWECLDFYPVAPKGMNGLDTSVTGQDIKHVLKVSLYSTRYEYYTVGEYDRDNDIYVPD 321

Query: 306 NTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRT 365
           NTS DGW+GLRYDYGNFYASK+F+DP K+RR+LWGWANESDS QDDV KGWAGIQ IPR 
Sbjct: 322 NTSVDGWAGLRYDYGNFYASKTFFDPDKQRRILWGWANESDSKQDDVQKGWAGIQLIPRK 381

Query: 366 VWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSL 425
           +WLDPNGKQL+QWP+ E+  LR + V M +Q L  G+++E+KG+TAAQADV+  FSF SL
Sbjct: 382 LWLDPNGKQLIQWPIEEIQLLRGQNVHMGSQVLNTGEHIEVKGVTAAQADVDATFSFKSL 441

Query: 426 DNAEAFDPSW--VDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 485
           D AE FDP+W  +DA  VC  +GST +GG+GPFG LTLASE LEE+TPVFFR+FK +   
Sbjct: 442 DRAEWFDPNWAKLDALDVCDWMGSTVRGGLGPFGFLTLASEKLEEYTPVFFRVFKTKDKL 501

Query: 486 VVLMCSDA-RSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITS 545
            VLMCSDA RSS+  +GLYKP FAG+VD+DLS KK+SLRSLID+SV+ESFG  G+TCITS
Sbjct: 502 KVLMCSDAKRSSTTAEGLYKPPFAGYVDVDLSDKKISLRSLIDNSVVESFGAHGRTCITS 561

Query: 546 RVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRS 576
           RVYP  A++ +AH+ VFNNGTE+IT++ L AWSM++
Sbjct: 562 RVYPKIAIYNNAHVFVFNNGTEAITIDSLDAWSMKA 585

BLAST of CmaCh17G005240 vs. ExPASy Swiss-Prot
Match: Q8W413 (Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3702 GN=CWINV4 PE=2 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 1.0e-204
Identity = 334/559 (59.75%), Postives = 423/559 (75.67%), Query Frame = 0

Query: 27  VQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQY 86
           + A H+IY ELQS     ++   +  LHR  FHFQP  HW+N PNGP+Y+KG+YHLFYQY
Sbjct: 27  IDAFHQIYEELQS-----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQY 86

Query: 87  NPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTG 146
           N +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG
Sbjct: 87  NTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 146

Query: 147 IDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLTLNGHWN 206
           ++    Q+QNYAIP + SDP+L +W+K D+NPI  P   +N SAFRDP+TAW + +GHW 
Sbjct: 147 VNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWR 206

Query: 207 TIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTS 266
           T++GS+ ++ G+AY+YRSRDF +W K + P+HS  +TGMWECPDF+PVS    R GLD  
Sbjct: 207 TVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLD 266

Query: 267 VMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 326
            +G   KHVLKVSL++TRYEYYTLGKY    D+Y+PD  + DGW GLR+DYGNFYASK+F
Sbjct: 267 YVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTF 326

Query: 327 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRLRR 386
           +D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WPV E+  LR 
Sbjct: 327 FDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRG 386

Query: 387 KIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV-DAQAVCSQLGS 446
             V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS
Sbjct: 387 NYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGS 446

Query: 447 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------K 506
             +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        K
Sbjct: 447 NVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAK 506

Query: 507 DGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLH 566
           D +YKP+FAGFVD+D++  ++SLRSLIDHSV+ESFG  GKT ITSRVYP KAV  +AHL+
Sbjct: 507 DRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLY 566

Query: 567 VFNNGTESITVEYLSAWSM 574
           VFNNGT+++T+E L+AW+M
Sbjct: 567 VFNNGTQTVTIESLNAWNM 580

BLAST of CmaCh17G005240 vs. ExPASy Swiss-Prot
Match: Q1PEF8 (Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3702 GN=CWINV2 PE=2 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 3.2e-198
Identity = 333/561 (59.36%), Postives = 415/561 (73.98%), Query Frame = 0

Query: 27  VQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQY 86
           V A HK++ +LQS     ++   +  LHRT +HFQP  HW+N PN PM +KG+YHLFYQY
Sbjct: 23  VDAFHKVFKKLQSKSTSLES---VSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQY 82

Query: 87  NPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTG 146
           NP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTG
Sbjct: 83  NPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTG 142

Query: 147 IDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLT-LNGHW 206
           I     Q+QNYAIP +LSDP+L  W+K D+NPIV P    N SAFRDP+TAW    +G+W
Sbjct: 143 ITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYW 202

Query: 207 NTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDT 266
             ++GS+ +  G+AY+Y+SRDF  W K ++P+HS   TGMWECPDF+PVS    + GLD 
Sbjct: 203 RMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDF 262

Query: 267 SVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKS 326
           S  G   KHVLKVSL++TRYEYYTLG Y    D+Y PD  + DGW GLR+DYGN+YASK+
Sbjct: 263 SYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKT 322

Query: 327 FYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRLR 386
           F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQL+ WP+ E+  LR
Sbjct: 323 FFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLR 382

Query: 387 RKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQ-AVCSQLG 446
            K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S+      +C+  G
Sbjct: 383 GKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKG 442

Query: 447 STAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL-------- 506
           S   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA+ SSL        
Sbjct: 443 SNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDTGTDA 502

Query: 507 KDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHL 566
           K+ +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSRVYPTKAV   AHL
Sbjct: 503 KERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHL 562

Query: 567 HVFNNGTESITVEYLSAWSMR 575
            VFNNG++ +TVE L+AW+M+
Sbjct: 563 FVFNNGSQPVTVESLNAWNMQ 580

BLAST of CmaCh17G005240 vs. ExPASy TrEMBL
Match: A0A6J1KWI1 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499336 PE=3 SV=1)

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 577/579 (99.65%), Postives = 578/579 (99.83%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARAD 580
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAR +
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARVN 579

BLAST of CmaCh17G005240 vs. ExPASy TrEMBL
Match: A0A6J1KY17 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499236 PE=3 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 568/576 (98.61%), Postives = 573/576 (99.48%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 575

BLAST of CmaCh17G005240 vs. ExPASy TrEMBL
Match: A0A6J1KW51 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 GN=LOC111499237 PE=3 SV=1)

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 568/576 (98.61%), Postives = 570/576 (98.96%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLP LKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPDLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           R VWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQ VEIKGITAAQADVEVIFSFS
Sbjct: 361 RMVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQIVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 575

BLAST of CmaCh17G005240 vs. ExPASy TrEMBL
Match: A0A6J1H3Y1 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460204 PE=3 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 547/579 (94.47%), Postives = 555/579 (95.85%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAIL GFSF SLFI NG VVVQALHKIYPELQSLE GND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILLGFSFYSLFIDNGLVVVQALHKIYPELQSLEKGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSIS+DLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPG KPVILYTGID+QNRQVQNYAIPANL DPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+ NGHWNTIIGSRSEQTGMAYLY S+DFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSFNGHWNTIIGSRSEQTGMAYLYTSKDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFG+DTSV GNW KHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGVDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWPVMELNRLRRK VMM++QKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKRVMMIHQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA---E 480
           SLD AEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFK    E
Sbjct: 421 SLDKAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNE 480

Query: 481 ANHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCI 540
            NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCI
Sbjct: 481 TNHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCI 540

Query: 541 TSRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           TSRVYPTKAVF DAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 TSRVYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSA 578

BLAST of CmaCh17G005240 vs. ExPASy TrEMBL
Match: A0A6J1H4M5 (beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460004 PE=3 SV=1)

HSP 1 Score: 1129.0 bits (2919), Expect = 0.0e+00
Identity = 541/579 (93.44%), Postives = 555/579 (95.85%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAIL GFSF SLF+ NGPVVVQALHK+YP+LQSL+NGN+  TEIKQLHRTGFHF
Sbjct: 45  MNFFTLLAILLGFSFYSLFVDNGPVVVQALHKLYPKLQSLKNGNN--TEIKQLHRTGFHF 104

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSIS+DLINWKSLKPALSPSK
Sbjct: 105 QPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISRDLINWKSLKPALSPSK 164

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPG KPVILYTGID+QNRQVQNYAIPANL DPHLTEWVK DNNPIV
Sbjct: 165 PFDINGCWSGSATVLPGFKPVILYTGIDSQNRQVQNYAIPANLFDPHLTEWVKPDNNPIV 224

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+ NGHWNTIIGSRSEQTGMAYLY S+DFLNWTKVEQPLHSV
Sbjct: 225 DPGTEVNSSAFRDPSTAWLSFNGHWNTIIGSRSEQTGMAYLYTSKDFLNWTKVEQPLHSV 284

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFG+DTSV GNW KHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 285 PNTGMWECPDFYPVSHFGRFGVDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 344

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 345 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 404

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWPVMELNRLRRK VMM++QKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 405 RTVWLDPNGKQLLQWPVMELNRLRRKRVMMIHQKLVKGQNVEIKGITAAQADVEVIFSFS 464

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKA---E 480
           SLD AEAFDPSWVDAQAVCSQLGS AQGGVGPFGLLTLASENLEEFTPVFFRIFK    E
Sbjct: 465 SLDKAEAFDPSWVDAQAVCSQLGSRAQGGVGPFGLLTLASENLEEFTPVFFRIFKGETNE 524

Query: 481 ANHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCI 540
            NHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCI
Sbjct: 525 TNHVVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCI 584

Query: 541 TSRVYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           TSRVYPTKAVF DAHLHVFNNGTESITVEYL+AWSMRSA
Sbjct: 585 TSRVYPTKAVFEDAHLHVFNNGTESITVEYLNAWSMRSA 621

BLAST of CmaCh17G005240 vs. NCBI nr
Match: XP_023006682.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 577/579 (99.65%), Postives = 578/579 (99.83%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARAD 580
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSAR +
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARVN 579

BLAST of CmaCh17G005240 vs. NCBI nr
Match: XP_023006546.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 568/576 (98.61%), Postives = 573/576 (99.48%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVV+QALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVIQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSR+FLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRNFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWP+MELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPIMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 575

BLAST of CmaCh17G005240 vs. NCBI nr
Match: XP_023006547.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima])

HSP 1 Score: 1182.9 bits (3059), Expect = 0.0e+00
Identity = 568/576 (98.61%), Postives = 570/576 (98.96%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLP LKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPDLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           R VWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQ VEIKGITAAQADVEVIFSFS
Sbjct: 361 RMVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQIVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 575

BLAST of CmaCh17G005240 vs. NCBI nr
Match: KAG6575223.1 (Beta-fructofuranosidase, insoluble isoenzyme 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1178.3 bits (3047), Expect = 0.0e+00
Identity = 571/622 (91.80%), Postives = 584/622 (93.89%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MN FTLLAIL GFS CSLF+ N P VVQALHKIYP+LQSLENGND  TEIKQLHRT FHF
Sbjct: 1   MNSFTLLAILLGFSCCSLFVDNDPAVVQALHKIYPKLQSLENGND--TEIKQLHRTSFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+ NGHWNTIIGSRSEQTGMAYLYRS+DFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSFNGHWNTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFG+DTSV GNW KHVLKVSLEMT YEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGVDTSVTGNWVKHVLKVSLEMTGYEYYTVGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWPVMELNRLRRKIVMM+NQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAE FDPSWVDAQAVCSQLGSTAQGG+GPFGLLTLASENLEEFTPVFFRIFKAE NH
Sbjct: 421 SLDNAETFDPSWVDAQAVCSQLGSTAQGGIGPFGLLTLASENLEEFTPVFFRIFKAETNH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSL+DGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLQDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSARADKAAIKAMPVNRVGDFGLALGI 600
           VYPTKAVF DAHLHVFNNGTESITV++LSAW       +KAAIKAMPVNR      A GI
Sbjct: 541 VYPTKAVFEDAHLHVFNNGTESITVDFLSAW-------NKAAIKAMPVNR------AHGI 600

Query: 601 SGRFTLFQTVDFSTIFAHAGAP 623
           S  FTLFQTVDFSTIFA A AP
Sbjct: 601 SSHFTLFQTVDFSTIFARASAP 607

BLAST of CmaCh17G005240 vs. NCBI nr
Match: XP_023547722.1 (beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1162.5 bits (3006), Expect = 0.0e+00
Identity = 557/576 (96.70%), Postives = 563/576 (97.74%), Query Frame = 0

Query: 1   MNFFTLLAILFGFSFCSLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHF 60
           MNFFTLLAIL GFSFCSLFI NG VVVQALHKIYPELQSLENGND TTEIKQLHRTGFHF
Sbjct: 1   MNFFTLLAILLGFSFCSLFIDNGLVVVQALHKIYPELQSLENGND-TTEIKQLHRTGFHF 60

Query: 61  QPHHHWMNGPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120
           QPH HWMN PNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK
Sbjct: 61  QPHRHWMNDPNGPMYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSK 120

Query: 121 PFDINGCWSGSATVLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIV 180
           PFDINGCWSGSATVLPGLKPVILYTGID+QNRQVQNYAIPANLSDPHLTEWVK DNNPIV
Sbjct: 121 PFDINGCWSGSATVLPGLKPVILYTGIDSQNRQVQNYAIPANLSDPHLTEWVKPDNNPIV 180

Query: 181 DPGTEVNSSAFRDPSTAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSV 240
           DPGTEVNSSAFRDPSTAWL+LNGHW TIIGSRSEQTGMAYLYRS+DFLNWTKVEQPLHSV
Sbjct: 181 DPGTEVNSSAFRDPSTAWLSLNGHWKTIIGSRSEQTGMAYLYRSKDFLNWTKVEQPLHSV 240

Query: 241 PNTGMWECPDFYPVSHFGRFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYV 300
           PNTGMWECPDFYPVSHFGRFGLDTSV GNW KHVLKVSLEMTRYEYYT+GKYYPMTDKYV
Sbjct: 241 PNTGMWECPDFYPVSHFGRFGLDTSVTGNWVKHVLKVSLEMTRYEYYTVGKYYPMTDKYV 300

Query: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360
           PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP
Sbjct: 301 PDNTSADGWSGLRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIP 360

Query: 361 RTVWLDPNGKQLLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFS 420
           RTVWLDPNGKQLLQWP+MELNRLRRKIVMM+NQKLVKGQNVEIKGITAAQADVEVIFSFS
Sbjct: 361 RTVWLDPNGKQLLQWPIMELNRLRRKIVMMINQKLVKGQNVEIKGITAAQADVEVIFSFS 420

Query: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH 480
           SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAE NH
Sbjct: 421 SLDNAEAFDPSWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAETNH 480

Query: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 540
           VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKL LRSLIDHSVIESFGGGGKTCITSR
Sbjct: 481 VVLMCSDARSSSLKDGLYKPTFAGFVDIDLSSKKLPLRSLIDHSVIESFGGGGKTCITSR 540

Query: 541 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMRSA 577
           VYPTKAVF DAHLHVFNNGTESITVEYLSAWSMRSA
Sbjct: 541 VYPTKAVFEDAHLHVFNNGTESITVEYLSAWSMRSA 575

BLAST of CmaCh17G005240 vs. TAIR 10
Match: AT2G36190.1 (cell wall invertase 4 )

HSP 1 Score: 714.5 bits (1843), Expect = 7.3e-206
Identity = 334/559 (59.75%), Postives = 423/559 (75.67%), Query Frame = 0

Query: 27  VQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQY 86
           + A H+IY ELQS     ++   +  LHR  FHFQP  HW+N PNGP+Y+KG+YHLFYQY
Sbjct: 27  IDAFHQIYEELQS-----ESVESVNHLHRPSFHFQPPKHWINDPNGPVYYKGLYHLFYQY 86

Query: 87  NPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTG 146
           N +GAVWGNI+WAHS+SKDL+NW++L+PALSPSK FDI G WSGS T++PG  P+ILYTG
Sbjct: 87  NTKGAVWGNIIWAHSVSKDLVNWEALEPALSPSKWFDIGGTWSGSITIVPGKGPIILYTG 146

Query: 147 IDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLTLNGHWN 206
           ++    Q+QNYAIP + SDP+L +W+K D+NPI  P   +N SAFRDP+TAW + +GHW 
Sbjct: 147 VNQNETQLQNYAIPEDPSDPYLRKWIKPDDNPIAIPDYTMNGSAFRDPTTAWFSKDGHWR 206

Query: 207 TIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDTS 266
           T++GS+ ++ G+AY+YRSRDF +W K + P+HS  +TGMWECPDF+PVS    R GLD  
Sbjct: 207 TVVGSKRKRRGIAYIYRSRDFKHWVKAKHPVHSKQSTGMWECPDFFPVSLTDFRNGLDLD 266

Query: 267 VMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKSF 326
            +G   KHVLKVSL++TRYEYYTLGKY    D+Y+PD  + DGW GLR+DYGNFYASK+F
Sbjct: 267 YVGPNTKHVLKVSLDITRYEYYTLGKYDLKKDRYIPDGNTPDGWEGLRFDYGNFYASKTF 326

Query: 327 YDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRLRR 386
           +D  K RR+LWGWANESD+V+DD+ KGWAG+Q IPRTV LD + KQL+ WPV E+  LR 
Sbjct: 327 FDYKKNRRILWGWANESDTVEDDILKGWAGLQVIPRTVLLDSSKKQLVFWPVEEIESLRG 386

Query: 387 KIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWV-DAQAVCSQLGS 446
             V M N  +  GQ +E+KGIT AQADVEV F   SL+ AE FDPS+      +C+  GS
Sbjct: 387 NYVRMNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEIFDPSFTWKPLELCNIKGS 446

Query: 447 TAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL--------K 506
             +GGVGPFGL+TLA+ +LEE+TPVFFR+F     H   VLMCSDAR SSL        K
Sbjct: 447 NVRGGVGPFGLITLATPDLEEYTPVFFRVFNDTKTHKPKVLMCSDARPSSLKQDTGLLAK 506

Query: 507 DGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHLH 566
           D +YKP+FAGFVD+D++  ++SLRSLIDHSV+ESFG  GKT ITSRVYP KAV  +AHL+
Sbjct: 507 DRMYKPSFAGFVDVDMADGRISLRSLIDHSVVESFGALGKTVITSRVYPVKAVKENAHLY 566

Query: 567 VFNNGTESITVEYLSAWSM 574
           VFNNGT+++T+E L+AW+M
Sbjct: 567 VFNNGTQTVTIESLNAWNM 580

BLAST of CmaCh17G005240 vs. TAIR 10
Match: AT3G52600.1 (cell wall invertase 2 )

HSP 1 Score: 693.0 bits (1787), Expect = 2.3e-199
Identity = 333/561 (59.36%), Postives = 415/561 (73.98%), Query Frame = 0

Query: 27  VQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYFKGIYHLFYQY 86
           V A HK++ +LQS     ++   +  LHRT +HFQP  HW+N PN PM +KG+YHLFYQY
Sbjct: 23  VDAFHKVFKKLQSKSTSLES---VSPLHRTAYHFQPPRHWINDPNAPMLYKGVYHLFYQY 82

Query: 87  NPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLPGLKPVILYTG 146
           NP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT +PG  PVILYTG
Sbjct: 83  NPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSATHVPGKGPVILYTG 142

Query: 147 IDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRDPSTAWLT-LNGHW 206
           I     Q+QNYAIP +LSDP+L  W+K D+NPIV P    N SAFRDP+TAW    +G+W
Sbjct: 143 ITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRDPTTAWFNKKDGYW 202

Query: 207 NTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPVSHFG-RFGLDT 266
             ++GS+ +  G+AY+Y+SRDF  W K ++P+HS   TGMWECPDF+PVS    + GLD 
Sbjct: 203 RMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFFPVSVTDKKNGLDF 262

Query: 267 SVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSGLRYDYGNFYASKS 326
           S  G   KHVLKVSL++TRYEYYTLG Y    D+Y PD  + DGW GLR+DYGN+YASK+
Sbjct: 263 SYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDGLRFDYGNYYASKT 322

Query: 327 FYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQLLQWPVMELNRLR 386
           F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQL+ WP+ E+  LR
Sbjct: 323 FFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQLVFWPIEEIESLR 382

Query: 387 RKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPSWVDAQ-AVCSQLG 446
            K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S+      +C+  G
Sbjct: 383 GKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDESFATKPLELCNLKG 442

Query: 447 STAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDARSSSL-------- 506
           S   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA+ SSL        
Sbjct: 443 SNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDAKPSSLKKDTGTDA 502

Query: 507 KDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAVFGDAHL 566
           K+ +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSRVYPTKAV   AHL
Sbjct: 503 KERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSRVYPTKAVGEKAHL 562

Query: 567 HVFNNGTESITVEYLSAWSMR 575
            VFNNG++ +TVE L+AW+M+
Sbjct: 563 FVFNNGSQPVTVESLNAWNMQ 580

BLAST of CmaCh17G005240 vs. TAIR 10
Match: AT3G13790.1 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 665.6 bits (1716), Expect = 3.9e-191
Identity = 322/570 (56.49%), Postives = 409/570 (71.75%), Query Frame = 0

Query: 17  SLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYF 76
           +L IGN  V ++A H +Y  L   ++ N  +  + Q +RTGFHFQP  +WMN PNGPM +
Sbjct: 16  TLLIGNYVVNLEASHHVYKRL--TQSTNTKSPSVNQPYRTGFHFQPPKNWMNDPNGPMIY 75

Query: 77  KGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLP 136
           KGIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP
Sbjct: 76  KGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILP 135

Query: 137 GLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPG--TEVNSSAFRDP 196
             KPVILYTGID +N+QVQN A P NLSDP+L EW KS  NP++ P     +N+S+FRDP
Sbjct: 136 NGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDP 195

Query: 197 STAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPV 256
           +TAWL  +  W  IIGS+  + G+A  Y S+DFL W K  +PLH    +GMWECPDF+PV
Sbjct: 196 TTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPV 255

Query: 257 SHFGRFGLDTSVMG---NWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSG 316
           + FG  G++TS  G      KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      + 
Sbjct: 256 TRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTA 315

Query: 317 LRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQ 376
            RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQ
Sbjct: 316 PRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQ 375

Query: 377 LLQWPVMELNRLRRKIVMMLNQKLVK-GQNVEIKGITAAQADVEVIFSFSSLDNAEAFDP 436
           L+QWPV E+ RLR K V  L  K++K G  +E+ G+TAAQADVEV+F    L+ A+  +P
Sbjct: 376 LIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEP 435

Query: 437 SWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEAN---HVVLMCSD 496
           SW D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD
Sbjct: 436 SWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSD 495

Query: 497 ARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAV 556
              SSLK+   K T+  FVDI+   + LSLR+LIDHSV+ESFGG G+ CITSRVYP  A+
Sbjct: 496 QSRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAI 555

Query: 557 FGDAHLHVFNNGTESITVEYLSAWSMRSAR 578
              +HL  FN G +S+ V  L+AWSM SA+
Sbjct: 556 GKSSHLFAFNYGYQSVDVLNLNAWSMNSAQ 582

BLAST of CmaCh17G005240 vs. TAIR 10
Match: AT3G13790.2 (Glycosyl hydrolases family 32 protein )

HSP 1 Score: 655.6 bits (1690), Expect = 4.0e-188
Identity = 320/570 (56.14%), Postives = 407/570 (71.40%), Query Frame = 0

Query: 17  SLFIGNGPVVVQALHKIYPELQSLENGNDTTTEIKQLHRTGFHFQPHHHWMNGPNGPMYF 76
           +L IGN  V ++A H +Y  L   ++ N  +  + Q +RTGFHFQP  +WM   NGPM +
Sbjct: 16  TLLIGNYVVNLEASHHVYKRL--TQSTNTKSPSVNQPYRTGFHFQPPKNWM---NGPMIY 75

Query: 77  KGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSATVLP 136
           KGIYHLFYQ+NP+GAVWGNIVWAHS S DLINW    PA+ PS PFDINGCWSGSAT+LP
Sbjct: 76  KGIYHLFYQWNPKGAVWGNIVWAHSTSTDLINWDPHPPAIFPSAPFDINGCWSGSATILP 135

Query: 137 GLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPG--TEVNSSAFRDP 196
             KPVILYTGID +N+QVQN A P NLSDP+L EW KS  NP++ P     +N+S+FRDP
Sbjct: 136 NGKPVILYTGIDPKNQQVQNIAEPKNLSDPYLREWKKSPLNPLMAPDAVNGINASSFRDP 195

Query: 197 STAWLTLNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFYPV 256
           +TAWL  +  W  IIGS+  + G+A  Y S+DFL W K  +PLH    +GMWECPDF+PV
Sbjct: 196 TTAWLGQDKKWRVIIGSKIHRRGLAITYTSKDFLKWEKSPEPLHYDDGSGMWECPDFFPV 255

Query: 257 SHFGRFGLDTSVMG---NWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSG 316
           + FG  G++TS  G      KHVLK+SL+ T+++YYT+G Y  + DK+VPDN      + 
Sbjct: 256 TRFGSNGVETSSFGEPNEILKHVLKISLDDTKHDYYTIGTYDRVKDKFVPDNGFKMDGTA 315

Query: 317 LRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQ 376
            RYDYG +YASK+F+D  K RR+LWGW NES SV+DDV KGW+GIQ IPR +WLD +GKQ
Sbjct: 316 PRYDYGKYYASKTFFDSAKNRRILWGWTNESSSVEDDVEKGWSGIQTIPRKIWLDRSGKQ 375

Query: 377 LLQWPVMELNRLRRKIVMMLNQKLVK-GQNVEIKGITAAQADVEVIFSFSSLDNAEAFDP 436
           L+QWPV E+ RLR K V  L  K++K G  +E+ G+TAAQADVEV+F    L+ A+  +P
Sbjct: 376 LIQWPVREVERLRTKQVKNLRNKVLKSGSRLEVYGVTAAQADVEVLFKVRDLEKADVIEP 435

Query: 437 SWVDAQAVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEAN---HVVLMCSD 496
           SW D Q +CS++  + + G+GPFGL+ LAS+NLEE+T V+FRIFKA  N   +VVLMCSD
Sbjct: 436 SWTDPQLICSKMNVSVKSGLGPFGLMVLASKNLEEYTSVYFRIFKARQNSNKYVVLMCSD 495

Query: 497 ARSSSLKDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSRVYPTKAV 556
              SSLK+   K T+  FVDI+   + LSLR+LIDHSV+ESFGG G+ CITSRVYP  A+
Sbjct: 496 QSRSSLKEDNDKTTYGAFVDIN-PHQPLSLRALIDHSVVESFGGKGRACITSRVYPKLAI 555

Query: 557 FGDAHLHVFNNGTESITVEYLSAWSMRSAR 578
              +HL  FN G +S+ V  L+AWSM SA+
Sbjct: 556 GKSSHLFAFNYGYQSVDVLNLNAWSMNSAQ 579

BLAST of CmaCh17G005240 vs. TAIR 10
Match: AT3G52600.2 (cell wall invertase 2 )

HSP 1 Score: 647.1 bits (1668), Expect = 1.4e-185
Identity = 312/514 (60.70%), Postives = 386/514 (75.10%), Query Frame = 0

Query: 74  MYFKGIYHLFYQYNPEGAVWGNIVWAHSISKDLINWKSLKPALSPSKPFDINGCWSGSAT 133
           M +KG+YHLFYQYNP+GAVWGNIVWAHS+SKDLINW++L+PA+ PSK FDING WSGSAT
Sbjct: 1   MLYKGVYHLFYQYNPKGAVWGNIVWAHSVSKDLINWEALEPAIYPSKWFDINGTWSGSAT 60

Query: 134 VLPGLKPVILYTGIDTQNRQVQNYAIPANLSDPHLTEWVKSDNNPIVDPGTEVNSSAFRD 193
            +PG  PVILYTGI     Q+QNYAIP +LSDP+L  W+K D+NPIV P    N SAFRD
Sbjct: 61  HVPGKGPVILYTGITENQTQIQNYAIPQDLSDPYLKTWIKPDDNPIVKPDNGENGSAFRD 120

Query: 194 PSTAWLT-LNGHWNTIIGSRSEQTGMAYLYRSRDFLNWTKVEQPLHSVPNTGMWECPDFY 253
           P+TAW    +G+W  ++GS+ +  G+AY+Y+SRDF  W K ++P+HS   TGMWECPDF+
Sbjct: 121 PTTAWFNKKDGYWRMLVGSKRKNRGIAYMYKSRDFKKWVKSKRPIHSRKKTGMWECPDFF 180

Query: 254 PVSHFG-RFGLDTSVMGNWFKHVLKVSLEMTRYEYYTLGKYYPMTDKYVPDNTSADGWSG 313
           PVS    + GLD S  G   KHVLKVSL++TRYEYYTLG Y    D+Y PD  + DGW G
Sbjct: 181 PVSVTDKKNGLDFSYDGPNAKHVLKVSLDLTRYEYYTLGTYDTKKDRYRPDGYTPDGWDG 240

Query: 314 LRYDYGNFYASKSFYDPMKKRRVLWGWANESDSVQDDVSKGWAGIQAIPRTVWLDPNGKQ 373
           LR+DYGN+YASK+F+D    RR+LWGWANESD+VQDD  KGWAGIQ IPRT+ LD +GKQ
Sbjct: 241 LRFDYGNYYASKTFFDDKTNRRILWGWANESDTVQDDTVKGWAGIQLIPRTILLDSSGKQ 300

Query: 374 LLQWPVMELNRLRRKIVMMLNQKLVKGQNVEIKGITAAQADVEVIFSFSSLDNAEAFDPS 433
           L+ WP+ E+  LR K V M NQK+  GQ  E++GIT AQ DV+V F+  +L+ AE FD S
Sbjct: 301 LVFWPIEEIESLRGKNVQMTNQKMEMGQRFEVQGITPAQVDVDVTFNVGNLEKAEKFDES 360

Query: 434 WVDAQ-AVCSQLGSTAQGGVGPFGLLTLASENLEEFTPVFFRIFKAEANH--VVLMCSDA 493
           +      +C+  GS   GGVGPFGL+TLA+ +LEE+TPVFFR+FK  A++   VLMCSDA
Sbjct: 361 FATKPLELCNLKGSNVNGGVGPFGLITLATSDLEEYTPVFFRVFKDAASNKPKVLMCSDA 420

Query: 494 RSSSL--------KDGLYKPTFAGFVDIDLSSKKLSLRSLIDHSVIESFGGGGKTCITSR 553
           + SSL        K+ +YKP+FAGFVD+ L   K+SLRSLIDHSV+ESFG  GKT ITSR
Sbjct: 421 KPSSLKKDTGTDAKERMYKPSFAGFVDVGLLDGKISLRSLIDHSVVESFGAKGKTVITSR 480

Query: 554 VYPTKAVFGDAHLHVFNNGTESITVEYLSAWSMR 575
           VYPTKAV   AHL VFNNG++ +TVE L+AW+M+
Sbjct: 481 VYPTKAVGEKAHLFVFNNGSQPVTVESLNAWNMQ 514

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P267926.6e-22867.39Beta-fructofuranosidase, insoluble isoenzyme 1 OS=Daucus carota OX=4039 GN=INV1 ... [more]
Q396939.0e-22565.74Beta-fructofuranosidase, insoluble isoenzyme 3 OS=Daucus carota OX=4039 GN=INV3 ... [more]
Q396922.4e-22264.93Beta-fructofuranosidase, insoluble isoenzyme 2 OS=Daucus carota OX=4039 GN=INV2 ... [more]
Q8W4131.0e-20459.75Beta-fructofuranosidase, insoluble isoenzyme CWINV4 OS=Arabidopsis thaliana OX=3... [more]
Q1PEF83.2e-19859.36Beta-fructofuranosidase, insoluble isoenzyme CWINV2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1KWI10.0e+0099.65beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1KY170.0e+0098.61beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1KW510.0e+0098.61beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1H3Y10.0e+0094.47beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=366... [more]
A0A6J1H4M50.0e+0093.44beta-fructofuranosidase, insoluble isoenzyme 1-like OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
XP_023006682.10.0e+0099.65beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
XP_023006546.10.0e+0098.61beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
XP_023006547.10.0e+0098.61beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita maxima][more]
KAG6575223.10.0e+0091.80Beta-fructofuranosidase, insoluble isoenzyme 1, partial [Cucurbita argyrosperma ... [more]
XP_023547722.10.0e+0096.70beta-fructofuranosidase, insoluble isoenzyme 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G36190.17.3e-20659.75cell wall invertase 4 [more]
AT3G52600.12.3e-19959.36cell wall invertase 2 [more]
AT3G13790.13.9e-19156.49Glycosyl hydrolases family 32 protein [more]
AT3G13790.24.0e-18856.14Glycosyl hydrolases family 32 protein [more]
AT3G52600.21.4e-18560.70cell wall invertase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001362Glycoside hydrolase, family 32SMARTSM00640glyco_32coord: 59..533
e-value: 3.7E-231
score: 783.9
NoneNo IPR availableGENE3D2.60.120.560coord: 390..573
e-value: 2.4E-78
score: 264.2
NoneNo IPR availablePANTHERPTHR31953BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATEDcoord: 8..577
NoneNo IPR availablePANTHERPTHR31953:SF65BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME 1-LIKEcoord: 8..577
NoneNo IPR availableCDDcd18624GH32_Fruct1-likecoord: 65..365
e-value: 3.85095E-170
score: 484.583
IPR013189Glycosyl hydrolase family 32, C-terminalPFAMPF08244Glyco_hydro_32Ccoord: 379..571
e-value: 1.3E-25
score: 90.3
IPR013148Glycosyl hydrolase family 32, N-terminalPFAMPF00251Glyco_hydro_32Ncoord: 59..376
e-value: 3.4E-102
score: 342.1
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilyGENE3D2.115.10.20Glycosyl hydrolase domain; family 43coord: 64..389
e-value: 1.5E-114
score: 384.8
IPR023296Glycosyl hydrolase, five-bladed beta-propellor domain superfamilySUPERFAMILY75005Arabinanase/levansucrase/invertasecoord: 51..377
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 376..576

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G005240.1CmaCh17G005240.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds