CmaCh17G003710 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G003710
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionEnoyl-CoA hydratase/isomerase family protein
LocationCma_Chr17: 2070159 .. 2073082 (+)
RNA-Seq ExpressionCmaCh17G003710
SyntenyCmaCh17G003710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGAATTCAACTGTAACTCTGCCCATGCCTACTTTTTTTTTATATTTATCCTTTGTTCATGATTTTCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACACCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTCGCCGGAATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGGTTTGTTATTTTCCGACTCAATTTTTTTGTAATCTCGACTGATTCTTGCTGCTAGGAATAGGATAAACACGGATTTAGGATCAATAAACCAAGTGATTTTCTGAAATCTCCTGCTAATTCTGTTCTATTGAAACTCCGAATTTCTGGAAAATTTCGTGATAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGTAATGTCCTCGACAAAATTCATATTCCGAATCTGTTGATCATTGAACCTTGAAAATTGTGTTCTTATTTCTGTTAAACTCGAGTTTCTCTTCGAATTTTTGTGCCAATAACTGTCTGGGGATGATTTTTTAAGTACTTTTTGGATGAACAAAGCTTGTGTAGTAGCATCCTGCAACTAGGGATGTCCAATGTTCATTTACACCATGGGGAATTGCTCCGAATGGGGTTTCCTCTTTACACAGGAAATGAGGAAGGAGGAGCAGGGAGAATTTTTGAAGAATATTCATCGTCTCCACTCTTGTTCCCGTCCTTGCCCCGAATGGGGTTTCCTCCCTACACAGGAAGTTGGGAAGGAGGAGAAAGGGGAATTTTCGAAGAATATTCGTTGTCCCTGCTCGGGGCGTTCCTCTTTACACAGGAAGTGGGGAAGGAGGAGGAAGTTGGTTTTTAGTGTTTGGTGGTGTTCGGCACAGGGGAGAACATCCAAGCCGTGGAATTCACACGAAAGGAAACGAGGAATTATCAACTAAAAAAGTGCTCTCTGAACATGAAAGTTGTTTTCCTCTTGGAAAAGTGTTCCTCGAAGATCCTTACCCTTCCACTTCCCCTTTCTTCCCATGTTTCCGCTGCTCTCGTTCTCGTCCTTGGCCTAAATAGGGCTTCCTCTTTACACAAGAAGTGGGGAAGGAGGAGGAAGTTGGTTTTTAGTGTTTGGTGGTGTTCTAGGGGAGAACATCCAAGCTGTGAAATTCACACGAGACGGAACGAGGAACGATCAACGAAAAAAGTGCTCTCTAAACGTGAAAGTTTTCCTACTGGGAAAGTCTCCCTCGACACAAAGTTCCTTACCCTTCCACCTTCACTTTCTTCCCTTGTTTCCAGTACTCTCGCTCCCGTCCTTGCCCCGTTTCTTTTATCTAAATGGAGAATAATTCTTGACGAGGAATCCTCATCCCAATACTAAACTTTGCGGGGATGGAAACGAAGCATTTCTACCTATACAACTGACTGTTTGTTTCGTTTGTTTGTTTGTTTTGTTGATGCAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAGTCTAATCGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGGTTAGTGGATTCTTCCCTTGTTTTATTCACATGTAATTTGATTTTAGTGGATTAGAGATAAAATGACTATTTTGAGAAGAATTCAGGCTGGTTTCTGAGCAATAAATGTTGGGTATTTTATGGATAATGGCTGAATATGAACTCATTGGATGGCAGTTTATGGTTGATTTGATATTACATTGTAAGGGTAGATTATAAATAACAATTATTTGTAAAGGAAGAATCCACAAGACATTCAGAACAGGATATATAAATGTTTAACATGACCTTCCCCCACCCAACTGAGTGGTGATGGTTCATGAACACAGTTAAGAGAGAGGTAAAATGTGAAGTCCTCACGTCGGTTAGAGAGAGGAACGAAAGGGTGTGGAAATCTTTTCCTAGTAGATGTGTTTTAAAACTTTGAGAACAAGCTTGAAAGGGAAAATCCAAAAAGAACAATATTTGTTAGTGGTGTGGCATGAGCTGTTAGAAATGGTGTCAGAGCAAGACATCGGGGGTGTGTCAACGAAGACGTTGGGTTCCTATGGAGATGGATTGTGAGATCCCACATCCGTTGGCCAGACACTGAGCAGTGTCAAACCAAAATACCCAACAATCGCCAGGTTGTCTCTTACCTGGCCATGGATTCAGCAGCAGTTTGAAGAGGGGAACAAAAC

mRNA sequence

TGAATTCAACTGTAACTCTGCCCATGCCTACTTTTTTTTTATATTTATCCTTTGTTCATGATTTTCTCGTTTTCCAATACGCAATCTTGCAAGAACTACCCCATGCCTACTTTTTTATTCAATCTCCACTCCCCAATTTGATCACACCGACTTCCACTTTCTGCTGTATTCGCTCCTGATTTCTTCGCCGGAATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAGTCTAATCGATGTAGCTTACTGTCTGTTTCTACACTGCAACAATAATAAGAAGAACTTGCCTCCTAAACGGTTAGTGGATTCTTCCCTTGTTTTATTCACATGTAATTTGATTTTAGTGGATTAGAGATAAAATGACTATTTTGAGAAGAATTCAGGCTGGTTTCTGAGCAATAAATGTTGGGTATTTTATGGATAATGGCTGAATATGAACTCATTGGATGGCAGTTTATGGTTGATTTGATATTACATTGTAAGGGTAGATTATAAATAACAATTATTTGTAAAGGAAGAATCCACAAGACATTCAGAACAGGATATATAAATGTTTAACATGACCTTCCCCCACCCAACTGAGTGGTGATGGTTCATGAACACAGTTAAGAGAGAGGTAAAATGTGAAGTCCTCACGTCGGTTAGAGAGAGGAACGAAAGGGTGTGGAAATCTTTTCCTAGTAGATGTGTTTTAAAACTTTGAGAACAAGCTTGAAAGGGAAAATCCAAAAAGAACAATATTTGTTAGTGGTGTGGCATGAGCTGTTAGAAATGGTGTCAGAGCAAGACATCGGGGGTGTGTCAACGAAGACGTTGGGTTCCTATGGAGATGGATTGTGAGATCCCACATCCGTTGGCCAGACACTGAGCAGTGTCAAACCAAAATACCCAACAATCGCCAGGTTGTCTCTTACCTGGCCATGGATTCAGCAGCAGTTTGAAGAGGGGAACAAAAC

Coding sequence (CDS)

ATGGATCGATCATCGTCGGAAAAGCTCATCCTCGTCAACCGGGAACCAAACGGGATCGCATTCGTTACGATCAACCGACCGAAGTCCTTGAATTCGTTAACGAGAGCGATGATGGCAGATTTGGCTCAGGCGTTTAAGAGCTTGGACCGCGACGAGTCCGTGCGAGTTATCGTGCTCTCTGGAACAGGCCGAGCCTTCTGTTCCGGTGTAGACCTTACTGCGGCGGAGGATGTTTTCAAAGGAGATGTGAAGGATGTTGAAGCCGATCCGGTTGTGCAAATGGAACGCTGCCGGAAGCCGATAATCGGAGCGGTTGCAGGTTTCGCCATCACCGCTGGATTTGAGATCGCCTTGGCTTGCGACATACTCATAGCTGCCAAAGGAGCTAAGTTCATCGATACTCATGCTAGATTTCGGATATTTCCTTCATGGGGATTGTCTCAGAAACTCCCGAGGATCATCGGAGTTAACAAAGCTCGTGAAGTATCCTTAACGGCGATGCCTCTAACTGCAGAGGAAGCAGAGAGGAAGGGTTTGGTGAATCACGTGGTCGACGGAGGTGAATTGTTGAAGAAAACTCGAGAGATTGCGGAGGCCATCTTGAAGAACAATCAGGACTTGGTGTTGAGATACAAGTCCGTCATTAACGATGGCCTGAAGCTGGATCTTGGCCATGCTCTTGCGCTGGAGAAGGAGAGAGCTCATGCATATTACAATGGAATGACCAAAGAGCAATTCAAGAAAATGCAGGAATTCATAGCTGGTCGGAGCTCCAAGAAGCCTTCCAAGTTATAG

Protein sequence

MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAGRSSKKPSKL
Homology
BLAST of CmaCh17G003710 vs. ExPASy Swiss-Prot
Match: Q6NL24 (Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECHIA PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 1.1e-112
Identity = 205/262 (78.24%), Postives = 231/262 (88.17%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++ SE LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARF IFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of CmaCh17G003710 vs. ExPASy Swiss-Prot
Match: P52046 (Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=crt PE=1 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.2e-31
Identity = 85/242 (35.12%), Postives = 137/242 (56.61%), Query Frame = 0

Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG-RAFCSGVDLTAAED 78
           +A VTINRPK+LN+L    + ++      ++ D  V  ++L+G G ++F +G D++  ++
Sbjct: 14  VAVVTINRPKALNALNSDTLKEMDYVIGEIENDSEVLAVILTGAGEKSFVAGADISEMKE 73

Query: 79  V--FKGDVKDVEADPVV-QMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDT 138
           +   +G    +  + V  ++E   KP+I AV GFA+  G EIA++CDI IA+  A+F   
Sbjct: 74  MNTIEGRKFGILGNKVFRRLELLEKPVIAAVNGFALGGGCEIAMSCDIRIASSNARFGQP 133

Query: 139 HARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTR 198
                I P +G +Q+L R++G+  A+++  TA  + A+EA R GLVN VV+  EL+   +
Sbjct: 134 EVGLGITPGFGGTQRLSRLVGMGMAKQLIFTAQNIKADEALRIGLVNKVVEPSELMNTAK 193

Query: 199 EIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIA 257
           EIA  I+ N    V   K  IN G++ D+  ALA E E     ++  T++Q   M  FI 
Sbjct: 194 EIANKIVSNAPVAVKLSKQAINRGMQCDIDTALAFESEAFGECFS--TEDQKDAMTAFIE 253

BLAST of CmaCh17G003710 vs. ExPASy Swiss-Prot
Match: Q1ZXF1 (Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=echs1 PE=3 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.3e-31
Identity = 86/242 (35.54%), Postives = 133/242 (54.96%), Query Frame = 0

Query: 19  IAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLTAAEDV 78
           +  VT+NRPKSLN+L+  +++++  A K    D+ V  I+++G+ +AF +G D+   E V
Sbjct: 34  VGLVTLNRPKSLNALSDGLISEINSAVKLFQEDKDVGSIIITGSEKAFAAGADIKEMEKV 93

Query: 79  FKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFIDTH 138
              D      D + Q   + + RKPII AV G+A+  G E+A+ CDI+IA + A F    
Sbjct: 94  TLPDA--YNNDLLAQWHDLTKIRKPIIAAVNGYALGGGCELAMMCDIIIAGEKAVFGQPE 153

Query: 139 ARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKKTRE 198
            +    P  G +Q+L R IG +KA E+ LT   LTA EAE+ GLV+ VV   ELL +  +
Sbjct: 154 IKLGTIPGCGGTQRLIRAIGKSKAMELVLTGNNLTAVEAEKAGLVSKVVPVEELLTEATK 213

Query: 199 IAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKKMQEFIAG 258
           +AE I   +Q  V   K  +N   +L L   +  E+   H+ +   T +Q + M  F+  
Sbjct: 214 MAEKIASYSQLTVAMAKEAVNASYELTLQEGIRFERRMFHSTFG--THDQKEGMNAFVEK 271

BLAST of CmaCh17G003710 vs. ExPASy Swiss-Prot
Match: A4YI89 (3-hydroxypropionyl-coenzyme A dehydratase OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=Msed_2001 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.1e-30
Identity = 78/222 (35.14%), Postives = 127/222 (57.21%), Query Frame = 0

Query: 14  REPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSGVDLT 73
           ++   + ++T+NRP  LN+L   ++ +L +A    + D  +RVI+++G G+AFC+G D+T
Sbjct: 9   KKEGNLFWITLNRPDKLNALNAKLLEELDRAVSQAESDPEIRVIIITGKGKAFCAGADIT 68

Query: 74  -----AAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 133
                   + +K   K  E   + ++E   KP I  + G+A+  G E+ALACDI IAA+ 
Sbjct: 69  QFNQLTPAEAWKFSKKGREI--MDKIEALSKPTIAMINGYALGGGLELALACDIRIAAEE 128

Query: 134 AKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 193
           A+         I+P +G +Q+L R+IG  +A E+ +T   +  ++AE+ GLVN VV    
Sbjct: 129 AQLGLPEINLGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEKYGLVNRVVPLAN 188

Query: 194 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALE 231
           L ++TR++AE I K +   +   K V+N GL   L   LALE
Sbjct: 189 LEQETRKLAEKIAKKSPISLALIKEVVNRGLDSPLLSGLALE 228

BLAST of CmaCh17G003710 vs. ExPASy Swiss-Prot
Match: P64017 (Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=echA8 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.8e-30
Identity = 83/249 (33.33%), Postives = 130/249 (52.21%), Query Frame = 0

Query: 10  ILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTGRAFCSG 69
           ILV R+   +  +T+NRP++LN+L   +M ++  A   LD D  +  I+++G+ +AF +G
Sbjct: 6   ILVERDQR-VGIITLNRPQALNALNSQVMNEVTSAATELDDDPDIGAIIITGSAKAFAAG 65

Query: 70  VDLTAAEDVFKGDVKDVEADPV-VQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKG 129
            D+    D+   D    +      ++   R P I AVAG+A+  G E+A+ CD+LIAA  
Sbjct: 66  ADIKEMADLTFADAFTADFFATWGKLAAVRTPTIAAVAGYALGGGCELAMMCDVLIAADT 125

Query: 130 AKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGE 189
           AKF     +  + P  G SQ+L R IG  KA ++ LT   + A EAER GLV+ VV   +
Sbjct: 126 AKFGQPEIKLGVLPGMGGSQRLTRAIGKAKAMDLILTGRTMDAAEAERSGLVSRVVPADD 185

Query: 190 LLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNGMTKEQFKK 249
           LL + R  A  I + +       K  +N   +  L   L  E+   H+ +   T++Q + 
Sbjct: 186 LLTEARATATTISQMSASAARMAKEAVNRAFESSLSEGLLYERRLFHSAF--ATEDQSEG 245

Query: 250 MQEFIAGRS 258
           M  FI  R+
Sbjct: 246 MAAFIEKRA 251

BLAST of CmaCh17G003710 vs. ExPASy TrEMBL
Match: A0A6J1L1R6 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. ExPASy TrEMBL
Match: A0A6J1L4H0 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499066 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 6.6e-140
Identity = 263/264 (99.62%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. ExPASy TrEMBL
Match: A0A6J1H7Z0 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111460389 PE=4 SV=1)

HSP 1 Score: 501.5 bits (1290), Expect = 2.1e-138
Identity = 259/264 (98.11%), Postives = 262/264 (99.24%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. ExPASy TrEMBL
Match: A0A6J1CAG2 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009856 PE=4 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.2e-129
Identity = 240/264 (90.91%), Postives = 254/264 (96.21%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD SS EKLILV+REPNGIAFVTINRPKSLNSLTR M+ DLA A KSLDRD+SVRVIVLS
Sbjct: 1   MDPSSPEKLILVDREPNGIAFVTINRPKSLNSLTRPMIVDLAHAIKSLDRDQSVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDL+AAE+VFKGDVKDVE+DPV+QMERCRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLSAAEEVFKGDVKDVESDPVLQMERCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAER+GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLSRIIGVNKAREVSLTAMPLTAEEAERRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+GGELLKK RE+AEAILKNNQD+VLRYKSVINDGL+LDLGHALALEKER HAYYNG
Sbjct: 181 NHVVEGGELLKKAREVAEAILKNNQDMVLRYKSVINDGLQLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. ExPASy TrEMBL
Match: A0A1S3C8X4 (probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497788 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 3.8e-127
Identity = 238/264 (90.15%), Postives = 251/264 (95.08%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS E LILVNRE NGIAF+TINRPKSLNSLTR MM DLA+AFKSLDRD+SVRVI+LS
Sbjct: 1   MDRSSPENLILVNRESNGIAFITINRPKSLNSLTRPMMIDLARAFKSLDRDDSVRVIILS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVE+DPV QME CRKPIIGA+AGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVESDPVAQMELCRKPIIGAIAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKL RIIGVNKAREVSLTAMPLTAEEAE++GLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLGRIIGVNKAREVSLTAMPLTAEEAEKRGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           N VV+G ELLKK RE+AEAILKNNQDLVLRYKSVINDGLKLDLG ALALEKERAHAYYNG
Sbjct: 181 NQVVEGSELLKKAREVAEAILKNNQDLVLRYKSVINDGLKLDLGQALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQF+KMQEFIAGRSSK+PSKL
Sbjct: 241 MTKEQFQKMQEFIAGRSSKRPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: XP_023006284.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 264/264 (100.00%), Postives = 264/264 (100.00%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: XP_023006283.1 (probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima])

HSP 1 Score: 506.5 bits (1303), Expect = 1.4e-139
Identity = 263/264 (99.62%), Postives = 263/264 (99.62%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: XP_022959404.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 501.5 bits (1290), Expect = 4.4e-138
Identity = 259/264 (98.11%), Postives = 262/264 (99.24%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFA+TAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAVTAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: KAG6575117.1 (putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 499.6 bits (1285), Expect = 1.7e-137
Identity = 259/264 (98.11%), Postives = 261/264 (98.86%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFK+LDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKTLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYY G
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYTG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. NCBI nr
Match: XP_023548460.1 (probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 499.6 bits (1285), Expect = 1.7e-137
Identity = 259/264 (98.11%), Postives = 261/264 (98.86%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MDRSS EKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS
Sbjct: 1   MDRSSPEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC
Sbjct: 61  GSGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DILIAAKGAKFIDTHARF IFPSWGLSQKLPRIIGV+KAREVSLTAMPLTAEEAERKGLV
Sbjct: 121 DILIAAKGAKFIDTHARFGIFPSWGLSQKLPRIIGVSKAREVSLTAMPLTAEEAERKGLV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKER HAYYNG
Sbjct: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERGHAYYNG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPSKL 265
           MTKEQFKKMQEFIAGRSSKKPSKL
Sbjct: 241 MTKEQFKKMQEFIAGRSSKKPSKL 264

BLAST of CmaCh17G003710 vs. TAIR 10
Match: AT4G16210.1 (enoyl-CoA hydratase/isomerase A )

HSP 1 Score: 407.5 bits (1046), Expect = 8.0e-114
Identity = 205/262 (78.24%), Postives = 231/262 (88.17%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLS 60
           MD++ SE LI V +E  GIA +TINRPKSLNSLTRAMM DLA+AFK +D DESV+V++ +
Sbjct: 1   MDQTVSENLIQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFT 60

Query: 61  GTGRAFCSGVDLTAAEDVFKGDVKDVEADPVVQMERCRKPIIGAVAGFAITAGFEIALAC 120
           G+GR+FCSGVDLTAAE VFKGDVKD E DPVVQMER RKPIIGA+ GFAITAGFE+ALAC
Sbjct: 61  GSGRSFCSGVDLTAAESVFKGDVKDPETDPVVQMERLRKPIIGAINGFAITAGFELALAC 120

Query: 121 DILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLV 180
           DIL+A++GAKF+DTHARF IFPSWGLSQKL RIIG NKAREVSLT+MPLTA+ A + G V
Sbjct: 121 DILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAGKLGFV 180

Query: 181 NHVVDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEKERAHAYYNG 240
           NHVV+ GE LKK REIAEAI+KN Q +VLR KSVINDGLKLDLGHAL LEKERAHAYY+G
Sbjct: 181 NHVVEEGEALKKAREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHALTLEKERAHAYYSG 240

Query: 241 MTKEQFKKMQEFIAGRSSKKPS 263
           MTKEQF+KMQEFIAGR SKKPS
Sbjct: 241 MTKEQFRKMQEFIAGRGSKKPS 262

BLAST of CmaCh17G003710 vs. TAIR 10
Match: AT1G60550.1 (enoyl-CoA hydratase/isomerase D )

HSP 1 Score: 114.4 bits (285), Expect = 1.4e-25
Identity = 79/214 (36.92%), Postives = 112/214 (52.34%), Query Frame = 0

Query: 18  GIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVLSGTG-RAFCSGVDLTAAE 77
           GIA +TINRP+  N+     + +L +AF     D SV VI+L+G G +AFCSG D     
Sbjct: 86  GIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGDQALRT 145

Query: 78  DVFKGDVKDV----EADPVVQMERCRKPIIGAVAGFAITAGFEIALACDILIAAKGAKFI 137
                D  DV      D  VQ+ R  KP+I  VAG+A+  G  + + CD+ IAA  A F 
Sbjct: 146 QDGYADPNDVGRLNVLDLQVQIRRLPKPVIAMVAGYAVGGGHILHMVCDLTIAADNAIFG 205

Query: 138 DTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHVVDGGELLKK 197
            T  +   F +   S  + R++G  KARE+       TA EAE+ GL+N VV   +L K+
Sbjct: 206 QTGPKVGSFDAGYGSSIMSRLVGPKKAREMWFMTRFYTASEAEKMGLINTVVPLEDLEKE 265

Query: 198 TREIAEAILKNNQDLVLRYKSVINDGLKLDLGHA 227
           T +    IL+N+   +   K+ +N    +D GHA
Sbjct: 266 TVKWCREILRNSPTAIRVLKAALN---AVDDGHA 296

BLAST of CmaCh17G003710 vs. TAIR 10
Match: AT5G43280.1 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 106.7 bits (265), Expect = 2.9e-23
Identity = 70/218 (32.11%), Postives = 107/218 (49.08%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAF-VTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL 60
           M   S + L ++ +  +   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGTGRAFCSGVDLTAAEDV----------------FKGDVKDVEADPVVQMERCRKPIIG 120
           SG G+ FCSG+DL +   +                 +  +K ++A  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AVAGFAITAGFEIALACDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVS 180
           A+ G  I  G ++  ACDI   ++ A F        I    G  Q+LP I+G   A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAERKGLVNHVVDG-GELLKKTREIAEAI 201
           LTA   +  EA+  GLV+ V     EL      IAE I
Sbjct: 181 LTARRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGI 217

BLAST of CmaCh17G003710 vs. TAIR 10
Match: AT4G16800.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 105.5 bits (262), Expect = 6.5e-23
Identity = 69/228 (30.26%), Postives = 116/228 (50.88%), Query Frame = 0

Query: 8   KLILVNREPNGIAFVTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL-SGTGRAF 67
           KL  ++   +GI  V ++RP + N++ + M+  L  AF+S+ +D S RV+++ S     F
Sbjct: 44  KLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGVF 103

Query: 68  CSGVDLTAAEDVFKGDVKDVEADPVVQ---MERCRKPIIGAVAGFAITAGFEIALACDIL 127
           C+G DL     +   +V             +E    P I A+ G A+  G E+ALACD+ 
Sbjct: 104 CAGADLKERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALGGGLEMALACDLR 163

Query: 128 IAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVSLTAMPLTAEEAERKGLVNHV 187
           I  + A F        I P  G +Q+L R++G + ++E+  T   + A EA  KGLVN  
Sbjct: 164 ICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDAIEAANKGLVNIC 223

Query: 188 VDGGELLKKTREIAEAILKNNQDLVLRYKSVINDGLKLDLGHALALEK 232
           V  GE  +K  E+A+ I +     +   K  I++G++ ++   L +E+
Sbjct: 224 VTAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVEE 271

BLAST of CmaCh17G003710 vs. TAIR 10
Match: AT5G43280.2 (delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 )

HSP 1 Score: 105.1 bits (261), Expect = 8.5e-23
Identity = 64/200 (32.00%), Postives = 101/200 (50.50%), Query Frame = 0

Query: 1   MDRSSSEKLILVNREPNGIAF-VTINRPKSLNSLTRAMMADLAQAFKSLDRDESVRVIVL 60
           M   S + L ++ +  +   F + INRP  LN+L+     +  +A  SLD++  V VI+L
Sbjct: 1   MTMESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIIL 60

Query: 61  SGTGRAFCSGVDLTAAEDV----------------FKGDVKDVEADPVVQMERCRKPIIG 120
           SG G+ FCSG+DL +   +                 +  +K ++A  +  +E+CRKP+I 
Sbjct: 61  SGAGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQA-AITAIEQCRKPVIA 120

Query: 121 AVAGFAITAGFEIALACDILIAAKGAKFIDTHARFRIFPSWGLSQKLPRIIGVNKAREVS 180
           A+ G  I  G ++  ACDI   ++ A F        I    G  Q+LP I+G   A E++
Sbjct: 121 AIHGACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELA 180

Query: 181 LTAMPLTAEEAERKGLVNHV 184
           LTA   +  EA+  GLV+ V
Sbjct: 181 LTARRFSGSEAKDLGLVSKV 199

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NL241.1e-11278.24Probable enoyl-CoA hydratase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=E... [more]
P520462.2e-3135.12Short-chain-enoyl-CoA hydratase OS=Clostridium acetobutylicum (strain ATCC 824 /... [more]
Q1ZXF18.3e-3135.54Probable enoyl-CoA hydratase, mitochondrial OS=Dictyostelium discoideum OX=44689... [more]
A4YI891.1e-3035.143-hydroxypropionyl-coenzyme A dehydratase OS=Metallosphaera sedula (strain ATCC ... [more]
P640171.8e-3033.33Probable enoyl-CoA hydratase echA8 OS=Mycobacterium bovis (strain ATCC BAA-935 /... [more]
Match NameE-valueIdentityDescription
A0A6J1L1R61.0e-140100.00probable enoyl-CoA hydratase 1, peroxisomal isoform X2 OS=Cucurbita maxima OX=36... [more]
A0A6J1L4H06.6e-14099.62probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Cucurbita maxima OX=36... [more]
A0A6J1H7Z02.1e-13898.11probable enoyl-CoA hydratase 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1CAG25.2e-12990.91probable enoyl-CoA hydratase 1, peroxisomal isoform X1 OS=Momordica charantia OX... [more]
A0A1S3C8X43.8e-12790.15probable enoyl-CoA hydratase 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103497... [more]
Match NameE-valueIdentityDescription
XP_023006284.12.1e-140100.00probable enoyl-CoA hydratase 1, peroxisomal isoform X2 [Cucurbita maxima][more]
XP_023006283.11.4e-13999.62probable enoyl-CoA hydratase 1, peroxisomal isoform X1 [Cucurbita maxima][more]
XP_022959404.14.4e-13898.11probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita moschata][more]
KAG6575117.11.7e-13798.11putative enoyl-CoA hydratase 1, peroxisomal, partial [Cucurbita argyrosperma sub... [more]
XP_023548460.11.7e-13798.11probable enoyl-CoA hydratase 1, peroxisomal [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G16210.18.0e-11478.24enoyl-CoA hydratase/isomerase A [more]
AT1G60550.11.4e-2536.92enoyl-CoA hydratase/isomerase D [more]
AT5G43280.12.9e-2332.11delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
AT4G16800.16.5e-2330.26ATP-dependent caseinolytic (Clp) protease/crotonase family protein [more]
AT5G43280.28.5e-2332.00delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Enoyl-CoA hydratase/isomerasePFAMPF00378ECH_1coord: 15..251
e-value: 5.3E-52
score: 176.6
NoneNo IPR availableGENE3D3.90.226.10coord: 9..260
e-value: 2.0E-67
score: 229.2
NoneNo IPR availablePANTHERPTHR43802ENOYL-COA HYDRATASEcoord: 4..260
NoneNo IPR availableCDDcd06558crotonase-likecoord: 10..200
e-value: 4.72394E-68
score: 207.028
IPR029045ClpP/crotonase-like domain superfamilySUPERFAMILY52096ClpP/crotonasecoord: 7..257

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G003710.1CmaCh17G003710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009536 plastid
molecular_function GO:0003824 catalytic activity