CmaCh17G001980 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G001980
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPyruvate kinase
LocationCma_Chr17: 1039692 .. 1044424 (-)
RNA-Seq ExpressionCmaCh17G001980
SyntenyCmaCh17G001980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGCTTGAAGCCGTTTTGGGCCCTGGATCTTCCGGCAGCCATTTCTGAAGCTCTGAACTTCCCACTTTTCCCCTCACAATGACACAATCTCTGCAAATTTTCACCTCCGCCACCCGCTCTCCCGGCATTTCTCTCTCCAAACACTCCCTCTCAAAACCCTCTCCGTCCACCGCCGCTTTCCGCTTCCCTCTCTCCTTCACAAAATCGTCAATCAGAGCCTCTTCCTCTCCGGATTCCAATCCTCTGTCATCTGCTTTCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCACCGGCGGAGGTGGAGCTCTGTCCTCTTCTGTTACTAGGGAGTTTGTGCCTGTTATGTCTGATTCCACCGCGATTGAGGTCGATACTGTGACGGAGGCTGAATTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACGAAGCTCGTGTGCACCATTGGCCCTGTTACCTGCGGATTCGAGCAGCTCGAGGCGCTCGCTGTTGGCGGGATGAATGTCGCAAGGATCAATATGTGCCATGGAACTCGAGACTGGCATCGGATGGTAATTGAACGCGTACGGAGGCTCAATGAGGAGAAGGGCTATGCGGTCGCTATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGTAAGTTCTTCGGTTCTTTATCGTTTTTCATCTTATTGTGTGATAATCAGTCGAATTCATTCTAATTTTTGCCCAAGATTTAAAAATTTCCGACTGCATTCGAATACTGGGATTTGCCTAGCTTACCCAACAGGCGATCATGATTATGTTGTTGATTCAGTCACTGAGAAACGTTTAGATTTCAAGTTTCTTCTGCCTGGAACTCTAAAATCCACTAAAGCTATTACACATTTTATGTTTTGAAACTAAGATGGGTCCTTTCATCATATAGTGTGTTGCGAACTTGTGTCGAGGCTTCTAAATATACTTTTATCATAAGCTACTTGTCACGAAATCTTGTCTGAAACTAAAAACTTTATCTTCCATTAATGAAAATGAGTTTCTAAAACGCGTCTATGGAGAGAGTATAGCCTTACTCCAACCAGTGTCTCGCACCGTCTATGGAGAAGAGAACGAGTGTCAGTGAGGACATTGGGCCCCAAAAAGGGATGAATTGTGAGATCCCACATTTATTGGAGAGAGGAACAAGTGCCCGGAGAATGCTAGGCCTTGAAGGGGGTGGATTGAAGGAGATGGATTGTGAGATCCCACATCGGTTGGAGAGGGAACAAATCATTTTTTACAAGGGTGTGGAACCTCTCTCTAACATATGTGTTTTAAAACTTTGAGGAAAAGTCCAAAGAAAATAATAACGGGTAAGAATCACTTCAAGAAGTTGGTGGGTCATGTTTAACTGCTTGCTCTTTTCTATGTAGGACGGTGAAATCTGGACATTCAGTGTCCGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTTAACTATGAAGGGTTTGCAGAAGGTTACTGGTTCTATCCTAAATCTAAAGTTCTCTCTGGCTTTTACTGAATGCATGCACTATCTAATATAGCCCCAGAATTGGATGTATGTGCGTTCATTATTGTAGTAAGTTCCATGATATTGGTATCAATCTTGAATTATAATTGATATTGTGTAGATGTGAGAGTGGGTGATGACCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATCGGTCCTGATGTTAAGTGCCTGTGTACCGACCCGGGATTGTTGTTGCCACGGGCTAATTTAACGTTTTGGAGGGATGGACATCTAGTTCGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGTAGGCGCCTCCCTCTTCCTTAAACTGTCATTTCCTTAGTTAATTGCATCTGCTTCTGGATCGTGGTCTCCATATGTTTGAGGTTAACCTTTTGTGCAGCCTAGTAGTGCAAATTGCCCTGATATAGTGCGGTATTCCCAGTCTAATGCAAGTTTTATGAATCCAGGATTGGTTGGATATTGATTTTGGAATTGCTGAAGGTGTTGATTTTCTTGCCATATCGTTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGGTAAGCATAATTATGGTGGTGTGCTGATTGTTCTTTTAGTCTGGGTCTGAAGCCAGGACCTCTCAGTCTAATAGTCCAAGCGCACCGCTAACAGATATTGTCCTTTTTGAGCTTTCCCCTCAAGATTTGATAAAAAAAAAATAAAAAACTTCATTCTCCTCCCTAACCAATGTATGGGATCTCACAATTCACCCCCTTCGAGACCTAGCGTCCTCGCTGACACTTGTTTCCTTCTCTAATCAATGTGGGACTCCCCAATCCACCCCCTTCAGGACCCAGCGTCTTTGCTGGCACACCGCCTCGTGTCCATCTCCCCTTCGAGGCTCAACCTTCTCGCTGACACATCGCCCGGTGTCTTGTTCTGGTACCATTTGTAATGGCCCAAGCCCACCACTAGCAGATGTTGTCCTCTTTGGGTTTTCCCTTTCGAGCTTCCGCTTAAGGTTTTTAAAACGCATTTGCTAGAGAGAGGTTTCCACATCCTTACAAATAATGCTTCGTTCAATTCCCCAACCGATGTGGGATCTCACAATCCACTCCCTTCGAGCCCAGTGTTCTTGCTGGCACTCGTTCCCTTCTCTAATCGATTTGGGACTTCCCAATCCACCCCCTTTTAGGACCTAGTGTCATTGCTGGCACACTACCTCATGTCCACCCCCTTCAGGACTCAGCCTCCTCGCTAGCACATCGCTCGGTGTCTGGCTTTGATACCATTTGTAACGGCTCAAGTCCACTGGTAACAAATATTGTCTTCTTTGGGCTTTCCTTTTCGGAGTTCCCTCAAAGTTTTTAAAACGTGTCTGCTAGGGAGAGGTTTTCTCACCCTGGTAAAGATTGTTTCGTTCTCCTCCCTAATCGATGTGAGATCTCACACTCATGGTTGGTTTTCACAGATTTAGTGGACCATATATATCTTTAACTATGTGTTAGTATATGGGCGCCTTCTAAATAATTTTTTTGTTTTTAAATTCGATATGCAGTGACATTTCCATCCTCGCGAAGATAGAGAGTCTGGACTCATTGAATAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCCATGGTAGCTAGAGGAGATTTAGGAGCACAAATACCACTGGAACAGGTGCCATTAGTCCAGCAAAAGGTGGTCCAACTATGCAGGCAGTTAAATAAACCAGTTATTGTTGCTTCACAGCTTCTCGAGTCGATGATCGAATATCCTACACCCACCAGAGCGGAAGTTGCTGATATTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTTTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTAGCTGTTTTGAGAAATGTCAGCTTAAGAATTGAGAAATGGTGGAGGGAAGAGAAACGCCATGAACCTATGGAACTCCCTGAAGTTGGATCTTCATCCTCAGATAGCATCTTAGAAGAGATCTGCAATTCCGCTGCTAAAATGGGTAAGCTTTATCATATTCTCCAACTATACTGTAGTTTAGATCTATGCTTTGGTACGGTAACCGCTCAAGCCCAAGCCCAAGTCCACCACTAGCAGATATTGTTCTTTTTGATTTGGATTTGGGTTCAAAAGGGAAAGCCCAAGTTCACCTCAAGCCCAAGTCCGAAAGGGAAAGCCCAAAAATGACCATATCTATTAGCGCTGGAGTTGGACTTGGATTCTTACGGGGAGAAGGAAAGAACACAAATACTACCTCATGTCATTACTCAATTCCAACATACAACATGTTACTTGAAGAGCCCTTCTTCATATGCTACTATCATCTATCCCTCACTTCCTTATAATGCTGCTCCTGTTGCTTATTCCTGTGTAGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGTCGACCCGATTGCCCGATCTTTGCATTTACAGAAACGACATCAGTGAGGAGACGTCTCAACCTGCAATGGGGTTTGATACCCTTCCGGATGAGCTTCTCAGACGACATGGAAAACAACCTCAACAAAATATTTTTGCTGCTCAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAAGAATTAAGGTGCCTCTCTATGTTTCTGCTAATGTTTTCATTCCTTTGAACTATTTAACTTGAACAAAGCTTCTGGATCTGTTATTTAGTTGCTAGGAAACCTATGGGAACTCTGATTCACTTGCTTTATTTCTTTTTATTCTCTAACATTTTTGTGGCTATCATATTAGTGACCTATGAACAAAAATGCTCAAACCAAGAATCGCCCAGACAACACTCCTACCTCTATCATCAATGAGTGATCATTTAGAGGTCTTAGCTAATGATCTGGGTTGTTTTTCTCTTGACGATGAAGCTTATCCTCTATCATCTTACTGGCCGATCTTCGCCCCTTATATTTTAAGATCATATCAAGTATTAAAATTGGTTCGAGAAAGAAAAAACAAAAAAGATGTCAAGCCATGACCTACGTTAGTTTTCTAGTTTAAAATTATATTAAAA

mRNA sequence

AAAGCTTGAAGCCGTTTTGGGCCCTGGATCTTCCGGCAGCCATTTCTGAAGCTCTGAACTTCCCACTTTTCCCCTCACAATGACACAATCTCTGCAAATTTTCACCTCCGCCACCCGCTCTCCCGGCATTTCTCTCTCCAAACACTCCCTCTCAAAACCCTCTCCGTCCACCGCCGCTTTCCGCTTCCCTCTCTCCTTCACAAAATCGTCAATCAGAGCCTCTTCCTCTCCGGATTCCAATCCTCTGTCATCTGCTTTCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCACCGGCGGAGGTGGAGCTCTGTCCTCTTCTGTTACTAGGGAGTTTGTGCCTGTTATGTCTGATTCCACCGCGATTGAGGTCGATACTGTGACGGAGGCTGAATTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACGAAGCTCGTGTGCACCATTGGCCCTGTTACCTGCGGATTCGAGCAGCTCGAGGCGCTCGCTGTTGGCGGGATGAATGTCGCAAGGATCAATATGTGCCATGGAACTCGAGACTGGCATCGGATGGTAATTGAACGCGTACGGAGGCTCAATGAGGAGAAGGGCTATGCGGTCGCTATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGACGGTGAAATCTGGACATTCAGTGTCCGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTTAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATCGGTCCTGATGTTAAGTGCCTGTGTACCGACCCGGGATTGTTGTTGCCACGGGCTAATTTAACGTTTTGGAGGGATGGACATCTAGTTCGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGAATTGCTGAAGGTGTTGATTTTCTTGCCATATCGTTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCCTCGCGAAGATAGAGAGTCTGGACTCATTGAATAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCCATGGTAGCTAGAGGAGATTTAGGAGCACAAATACCACTGGAACAGGTGCCATTAGTCCAGCAAAAGGTGGTCCAACTATGCAGGCAGTTAAATAAACCAGTTATTGTTGCTTCACAGCTTCTCGAGTCGATGATCGAATATCCTACACCCACCAGAGCGGAAGTTGCTGATATTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTTTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTAGCTGTTTTGAGAAATGTCAGCTTAAGAATTGAGAAATGGTGGAGGGAAGAGAAACGCCATGAACCTATGGAACTCCCTGAAGTTGGATCTTCATCCTCAGATAGCATCTTAGAAGAGATCTGCAATTCCGCTGCTAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGTCGACCCGATTGCCCGATCTTTGCATTTACAGAAACGACATCAGTGAGGAGACGTCTCAACCTGCAATGGGGTTTGATACCCTTCCGGATGAGCTTCTCAGACGACATGGAAAACAACCTCAACAAAATATTTTTGCTGCTCAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAAGAATTAAGGTGCCTCTCTATGTTTCTGCTAATGTTTTCATTCCTTTGAACTATTTAACTTGAACAAAGCTTCTGGATCTGTTATTTAGTTGCTAGGAAACCTATGGGAACTCTGATTCACTTGCTTTATTTCTTTTTATTCTCTAACATTTTTGTGGCTATCATATTAGTGACCTATGAACAAAAATGCTCAAACCAAGAATCGCCCAGACAACACTCCTACCTCTATCATCAATGAGTGATCATTTAGAGGTCTTAGCTAATGATCTGGGTTGTTTTTCTCTTGACGATGAAGCTTATCCTCTATCATCTTACTGGCCGATCTTCGCCCCTTATATTTTAAGATCATATCAAGTATTAAAATTGGTTCGAGAAAGAAAAAACAAAAAAGATGTCAAGCCATGACCTACGTTAGTTTTCTAGTTTAAAATTATATTAAAA

Coding sequence (CDS)

ATGACACAATCTCTGCAAATTTTCACCTCCGCCACCCGCTCTCCCGGCATTTCTCTCTCCAAACACTCCCTCTCAAAACCCTCTCCGTCCACCGCCGCTTTCCGCTTCCCTCTCTCCTTCACAAAATCGTCAATCAGAGCCTCTTCCTCTCCGGATTCCAATCCTCTGTCATCTGCTTTCACTTCCCAAGTCTTGGTTTCTGAAAATGGCTCCACCGGCGGAGGTGGAGCTCTGTCCTCTTCTGTTACTAGGGAGTTTGTGCCTGTTATGTCTGATTCCACCGCGATTGAGGTCGATACTGTGACGGAGGCTGAATTGAAGGAGAATGGCTTCAGGAGCACCAGGAGGACGAAGCTCGTGTGCACCATTGGCCCTGTTACCTGCGGATTCGAGCAGCTCGAGGCGCTCGCTGTTGGCGGGATGAATGTCGCAAGGATCAATATGTGCCATGGAACTCGAGACTGGCATCGGATGGTAATTGAACGCGTACGGAGGCTCAATGAGGAGAAGGGCTATGCGGTCGCTATCATGATGGATACTGAAGGGAGTGAAATTCACATGGGTGATCTTGGTGGAGCTTCTTCGGCTAAAGCGGAAGACGGTGAAATCTGGACATTCAGTGTCCGAGCTTTTGATTCAACACTCCCAGAACGCACTATTAATGTTAACTATGAAGGGTTTGCAGAAGATGTGAGAGTGGGTGATGACCTCCTTGTTGATGGTGGAATGGTGAGGTTTGAGGTGATCGAAAAGATCGGTCCTGATGTTAAGTGCCTGTGTACCGACCCGGGATTGTTGTTGCCACGGGCTAATTTAACGTTTTGGAGGGATGGACATCTAGTTCGAGAACGTAATGCCATGCTCCCTACAATTTCTTCTAAGGATTGGTTGGATATTGATTTTGGAATTGCTGAAGGTGTTGATTTTCTTGCCATATCGTTTGTCAAGTCTGCTGAAGTGATTAAACATCTCAAAAGCTATATTGCTGCACGTTCTCGTGGCAGTGACATTTCCATCCTCGCGAAGATAGAGAGTCTGGACTCATTGAATAACTTGGAAGAAATTATTTTAGCATCAGATGGAGCCATGGTAGCTAGAGGAGATTTAGGAGCACAAATACCACTGGAACAGGTGCCATTAGTCCAGCAAAAGGTGGTCCAACTATGCAGGCAGTTAAATAAACCAGTTATTGTTGCTTCACAGCTTCTCGAGTCGATGATCGAATATCCTACACCCACCAGAGCGGAAGTTGCTGATATTTCCGAGGCGGTTAGGCAGCGATCAGATGCTCTAATGCTTTCTGGTGAGTCCGCCATGGGCCAGTACCCTGACAAGGCATTAGCTGTTTTGAGAAATGTCAGCTTAAGAATTGAGAAATGGTGGAGGGAAGAGAAACGCCATGAACCTATGGAACTCCCTGAAGTTGGATCTTCATCCTCAGATAGCATCTTAGAAGAGATCTGCAATTCCGCTGCTAAAATGGCCAATAATTTGGAGGTGGATGCAATTTTCGTCTACACAAAGACAGGCCACATGGCATCTCTCCTGTCCCGATGTCGACCCGATTGCCCGATCTTTGCATTTACAGAAACGACATCAGTGAGGAGACGTCTCAACCTGCAATGGGGTTTGATACCCTTCCGGATGAGCTTCTCAGACGACATGGAAAACAACCTCAACAAAATATTTTTGCTGCTCAAAGCCAGAAACTTGATCAAATCAGGTGACCTTGTCATTGCTGTCTCAGACATGTTGCAGTCTATCCAAGTTATGAATGTTCCATAA

Protein sequence

MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAFTSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Homology
BLAST of CmaCh17G001980 vs. ExPASy Swiss-Prot
Match: Q43117 (Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 6.4e-265
Identity = 481/597 (80.57%), Postives = 527/597 (88.27%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSK-PSP-STAAFRFPLSFTKS-SIRASSSPDSNPLS 60
           M+QSL        SP ++ +K    K P P  T+  R+P++  KS SI+AS+SP     S
Sbjct: 1   MSQSLHF------SPNLTFAKQPFPKLPLPFPTSNSRYPVNNYKSLSIKASTSP-----S 60

Query: 61  SAFTSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRT 120
           S+   QVLV++NG TG  G L ++  +     +SD ++IEVD VTE ELKENGFRSTRRT
Sbjct: 61  SSSDPQVLVADNG-TGNSGVLYNNNNKSV--TVSDPSSIEVDAVTETELKENGFRSTRRT 120

Query: 121 KLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIM 180
           KLVCTIGP TCGFE+LEALAVGGMNVARINMCHGTR+WH+ VIERVRRLNEEKG+AVAIM
Sbjct: 121 KLVCTIGPATCGFEELEALAVGGMNVARINMCHGTREWHKSVIERVRRLNEEKGFAVAIM 180

Query: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDL 240
           MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRA+DS  PERTINVNY+GFAEDV+VGD+L
Sbjct: 181 MDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAYDSPRPERTINVNYDGFAEDVKVGDEL 240

Query: 241 LVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWL 300
           LVDGGMVRFEVIEKIGPDVKC CTDPGLLLPRANLTFWRDG LVRERNAMLPTISSKDWL
Sbjct: 241 LVDGGMVRFEVIEKIGPDVKCRCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSKDWL 300

Query: 301 DIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIIL 360
           DIDFGIAEGVDF+AISFVKSAEVI HLKSYIAARSR SDI+++AKIES+DSL NLEEII 
Sbjct: 301 DIDFGIAEGVDFIAISFVKSAEVINHLKSYIAARSRDSDIAVIAKIESIDSLKNLEEIIR 360

Query: 361 ASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEV 420
           ASDGAMVARGDLGAQIPLEQVP  QQ +VQ+CRQLNKPVIVASQLLESMIEYPTPTRAEV
Sbjct: 361 ASDGAMVARGDLGAQIPLEQVPSAQQNIVQVCRQLNKPVIVASQLLESMIEYPTPTRAEV 420

Query: 421 ADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGS 480
           AD+SEAVRQR+DALMLSGESAMGQYP+KALAVLR+VS+RIEKWWREEK HE MELP +GS
Sbjct: 421 ADVSEAVRQRADALMLSGESAMGQYPEKALAVLRSVSVRIEKWWREEKHHEAMELPAIGS 480

Query: 481 SSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRR 540
           + SDSI EEICNSAAKMANNL VDA+FVYTK GHMASLLSRCRPDCPIFAFT TTSVRRR
Sbjct: 481 TYSDSISEEICNSAAKMANNLGVDALFVYTKDGHMASLLSRCRPDCPIFAFTTTTSVRRR 540

Query: 541 LNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           LNLQWGLIPFR+SF+DDME+NLNK F LLKAR +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 LNLQWGLIPFRLSFADDMESNLNKTFSLLKARGMIKSGDLVIAVSDMLQSIQVMNVP 583

BLAST of CmaCh17G001980 vs. ExPASy Swiss-Prot
Match: Q9LIK0 (Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PKP1 PE=1 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 1.2e-263
Identity = 474/600 (79.00%), Postives = 528/600 (88.00%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHS-LSKPSPSTAAFRFPLSFTK-SSIRASS----SPDSN 60
           M+QS+Q F++ + +P +    HS  ++P  S +  RFPL+  K +SIRASS    SPD +
Sbjct: 1   MSQSIQ-FSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLD 60

Query: 61  PLSSAFTSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRST 120
             SS+ +SQVL+S NG+   G   S   +     V +D++ IEVDTVTEAELKENGFRST
Sbjct: 61  SSSSSSSSQVLLSPNGT---GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRST 120

Query: 121 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAV 180
           RRTKL+CTIGP TCGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLNEEKG+AV
Sbjct: 121 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAV 180

Query: 181 AIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVG 240
           AIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVG
Sbjct: 181 AIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVG 240

Query: 241 DDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSK 300
           D+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSK
Sbjct: 241 DELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSK 300

Query: 301 DWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEE 360
           DWLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I ++AKIES+DSL NLEE
Sbjct: 301 DWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEE 360

Query: 361 IILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTR 420
           IILASDGAMVARGDLGAQIPLEQVP  QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTR
Sbjct: 361 IILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTR 420

Query: 421 AEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPE 480
           AEVAD+SEAVRQRSDALMLSGESAMGQ+PDKAL VLR VSLRIE+WWREEKRHE + L  
Sbjct: 421 AEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQA 480

Query: 481 VGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSV 540
           +GSS SD I EEICNSAAKMANNL VDA+FVYT +GHMASL+SRCRPDCPIFAFT TTSV
Sbjct: 481 IGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSV 540

Query: 541 RRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           RRRLNLQWGLIPFR+SFSDDME+NLNK F LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of CmaCh17G001980 vs. ExPASy Swiss-Prot
Match: Q40545 (Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 907.1 bits (2343), Expect = 1.0e-262
Identity = 477/604 (78.97%), Postives = 531/604 (87.91%), Query Frame = 0

Query: 1   MTQSLQIF-TSATRSPGISLSKHSLSKPS--PSTAAFRFPLSFTKSSIRA----SSSPDS 60
           M+Q+L  F +S++RSP    +  ++S+PS  PST + R      K S+R     +SS  +
Sbjct: 1   MSQALNFFVSSSSRSP----ATFTISRPSVFPSTGSLRL---LVKKSLRTLVVEASSAAA 60

Query: 61  NPLSSAFTSQVLVSENGSTGGGGALSSSVTREF---VPVMSDSTAIEVDTVTEAELKENG 120
           + L    +S VLVSENGS   GG LSS+ T+E+       +DS++IEVDTVTEAELKENG
Sbjct: 61  SDLDEPQSSPVLVSENGS---GGVLSSA-TQEYGRNAAPGTDSSSIEVDTVTEAELKENG 120

Query: 121 FRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEK 180
           FRSTRRTKL+CTIGP TCGFEQLE LA GGMNVARINMCHGTR+WHRMVIER+RRLNEEK
Sbjct: 121 FRSTRRTKLICTIGPATCGFEQLERLAEGGMNVARINMCHGTREWHRMVIERLRRLNEEK 180

Query: 181 GYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAED 240
           G+AVAIMMDTEGSEIHMGDLGGASSAKAEDGEIW F+VR+FD  LPERT+ VNY+GFAED
Sbjct: 181 GFAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWNFTVRSFDPPLPERTVTVNYDGFAED 240

Query: 241 VRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPT 300
           V+VGD+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPT
Sbjct: 241 VKVGDELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGKLVRERNAMLPT 300

Query: 301 ISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLN 360
           ISSKDWLDIDFGIAEGVDF+A+SFVKSAEVIKHLKSYI AR+R SDIS++AKIES+DSL 
Sbjct: 301 ISSKDWLDIDFGIAEGVDFIAVSFVKSAEVIKHLKSYIQARARDSDISVIAKIESIDSLK 360

Query: 361 NLEEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYP 420
           NLEEII ASDGAMVARGDLGAQIPLEQVP  QQK+VQ+CRQLN+PVIVASQLLESMIEYP
Sbjct: 361 NLEEIIQASDGAMVARGDLGAQIPLEQVPSEQQKIVQICRQLNRPVIVASQLLESMIEYP 420

Query: 421 TPTRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPM 480
            PTRAEVAD+SEAVRQR DALMLSGESAMGQ+P+KAL VLR+VSLRIE+ WRE+KRHE +
Sbjct: 421 IPTRAEVADVSEAVRQRGDALMLSGESAMGQFPEKALTVLRSVSLRIERMWREQKRHEVI 480

Query: 481 ELPEVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTE 540
           ELP + SS SDSI EEICNSAAKMANNLEVDA+FVYTK GHMASLLSRCRPDCPIFAFT 
Sbjct: 481 ELPSIASSFSDSISEEICNSAAKMANNLEVDALFVYTKNGHMASLLSRCRPDCPIFAFTT 540

Query: 541 TTSVRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQV 595
           TTSVRRRLNLQWGL+PFR+SFSDDME+NLNK F LLKAR +IKSGDL+IAVSDMLQSIQV
Sbjct: 541 TTSVRRRLNLQWGLMPFRLSFSDDMESNLNKTFSLLKARGMIKSGDLIIAVSDMLQSIQV 593

BLAST of CmaCh17G001980 vs. ExPASy Swiss-Prot
Match: Q40546 (Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.3e-100
Identity = 213/477 (44.65%), Postives = 296/477 (62.05%), Query Frame = 0

Query: 113 STRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEE-KG 172
           S R+TK+VCTIGP T   E +  LA  GMNVAR+NM HG    H+  I+ V+  N + + 
Sbjct: 89  SPRKTKIVCTIGPSTSSREMIWKLAEAGMNVARLNMSHGDHASHQRTIDLVKEYNAQFED 148

Query: 173 YAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDV 232
             +AIM+DT+G E+  GD+        ++G+ + FS++   ST  E T++VNY+ F  DV
Sbjct: 149 KVIAIMLDTKGPEVISGDV--PKPILLKEGQEFNFSIKRGVST--EDTVSVNYDDFINDV 208

Query: 233 RVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTI 292
             GD LLVDGGM+   V  K    VKC   D G L  R +L        VR ++A LP+I
Sbjct: 209 EAGDILLVDGGMMSLAVKSKTSDIVKCEVIDGGELKSRRHLN-------VRGKSATLPSI 268

Query: 293 SSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNN 352
           + KDW DI FG+   VDF A+SFVK A+V+  LK Y+  +S  +DI ++ KIES DS+ N
Sbjct: 269 TEKDWDDIKFGVNNQVDFYAVSFVKDAKVVHELKDYL--KSCNADIHVIVKIESADSIPN 328

Query: 353 LEEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPT 412
           L  II ASDGAMVARGDLGA++P+E+VPL+Q+ +++ C+ + KPVIVA+ +LESMI++PT
Sbjct: 329 LHSIISASDGAMVARGDLGAELPIEEVPLLQEDIIRRCQSMQKPVIVATNMLESMIDHPT 388

Query: 413 PTRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPME 472
           PTRAEV+DIS AVR+ +DA+MLSGE+A G+YP KA+ V+  V+LR      E    +   
Sbjct: 389 PTRAEVSDISIAVREGADAVMLSGETAHGKYPLKAVKVMHIVALR-----TESSLQKSTS 448

Query: 473 LPEVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTET 532
            P   ++    + E     ++ MAN L    I V+T+TG MA +LS  RP   +FAFT  
Sbjct: 449 SPSQSAAYKSHMGEMFAFHSSSMANTLST-PIIVFTRTGSMAIILSHNRPSSTVFAFTNN 508

Query: 533 TSVRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSI 589
             V++RL L  G++P  M FS D E   ++   LL +++L+K G  V  V    Q I
Sbjct: 509 ERVKQRLALYHGVVPIYMEFSSDAEETFSRAIKLLLSKSLVKDGQYVTLVQSGAQPI 546

BLAST of CmaCh17G001980 vs. ExPASy Swiss-Prot
Match: Q9FLW9 (Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 7.2e-99
Identity = 208/475 (43.79%), Postives = 290/475 (61.05%), Query Frame = 0

Query: 115 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLN-EEKGYA 174
           R+TK+VCT+GP T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 175 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 234
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 235 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 294
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 295 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLE 354
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI ++ KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 355 EIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 414
            II ASDGAMVARGDLGA++P+E+VP++Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 415 RAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELP 474
           RAEV+DI+ AVR+ +DA+MLSGE+A G++P KA  V+  V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 475 EVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTS 534
            +G +  + + E     A  M+N L    + V+T+TG MA LLS  RP   I+AFT    
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 535 VRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSI 589
           +++RL L  G+ P  M F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of CmaCh17G001980 vs. ExPASy TrEMBL
Match: A0A6J1L0W9 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI
Sbjct: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of CmaCh17G001980 vs. ExPASy TrEMBL
Match: A0A6J1KWK2 (Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 594/596 (99.66%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG--SDISILAKIESLDSLNNLEEIILA 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG  SDISILAKIESLDSLNNLEEIILA
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGRCSDISILAKIESLDSLNNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480
           DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540
           SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of CmaCh17G001980 vs. ExPASy TrEMBL
Match: A0A6J1H3D9 (Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111460037 PE=3 SV=1)

HSP 1 Score: 1124.0 bits (2906), Expect = 0.0e+00
Identity = 584/596 (97.99%), Postives = 588/596 (98.66%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTSATRSPGISLSKHSLSKPSPSTAAFRFPLSF KSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFPKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLV ENGST GGGA+SSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVPENGSTVGGGAVSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNY GFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYGGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG--SDISILAKIESLDSLNNLEEIILA 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG   DISI+AKIESLDSLNNLEEII+A
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGRCCDISIIAKIESLDSLNNLEEIIVA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480
           DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540
           SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of CmaCh17G001980 vs. ExPASy TrEMBL
Match: A0A5A7UTK2 (Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G001510 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.0e-306
Identity = 551/594 (92.76%), Postives = 568/594 (95.62%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTS+     ISL KHS+SKP  STAAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG +SSS TREFV V SDST+I+VD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP +CGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD+
Sbjct: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVGSS S
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of CmaCh17G001980 vs. ExPASy TrEMBL
Match: A0A1S3BLQ9 (Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1)

HSP 1 Score: 1060.8 bits (2742), Expect = 2.0e-306
Identity = 551/594 (92.76%), Postives = 568/594 (95.62%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTS+     ISL KHS+SKP  STAAFRFP SF+KSSIRASSS D NPLSS+ 
Sbjct: 1   MTQSLQLFTSS----AISLPKHSISKPFSSTAAFRFPFSFSKSSIRASSS-DLNPLSSSS 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGS+ GGG +SSS TREFV V SDST+I+VD VTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSSSGGGVVSSSATREFVSVASDSTSIDVDAVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGP +CGFEQLEALAVGGMNVARINMCHGTRDWHR VIERVRRLN+EKGYAVAIMMDT
Sbjct: 121 CTIGPASCGFEQLEALAVGGMNVARINMCHGTRDWHRTVIERVRRLNDEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSL NLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLKNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVAD+
Sbjct: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADV 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLR+VSLRIEKWWR+EKRHEPMELPEVGSS S
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRSVSLRIEKWWRDEKRHEPMELPEVGSSFS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYT +GHMASLLSRCRPDCPIFAFT TTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTTSGHMASLLSRCRPDCPIFAFTSTTSVRRRLNL 540

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFR+SFSDDMENNLNK FLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRLSFSDDMENNLNKTFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 589

BLAST of CmaCh17G001980 vs. NCBI nr
Match: XP_023006710.1 (pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 594/594 (100.00%), Postives = 594/594 (100.00%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI
Sbjct: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of CmaCh17G001980 vs. NCBI nr
Match: XP_023006707.1 (pyruvate kinase isozyme A, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 594/596 (99.66%), Postives = 594/596 (99.66%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG--SDISILAKIESLDSLNNLEEIILA 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRG  SDISILAKIESLDSLNNLEEIILA
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGRCSDISILAKIESLDSLNNLEEIILA 360

Query: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420
           SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA
Sbjct: 361 SDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVA 420

Query: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480
           DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS
Sbjct: 421 DISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSS 480

Query: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540
           SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL
Sbjct: 481 SSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRL 540

Query: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 NLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of CmaCh17G001980 vs. NCBI nr
Match: XP_023548664.1 (pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 587/594 (98.82%), Postives = 592/594 (99.66%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF
Sbjct: 1   MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGST GGGA+SSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 61  TSQVLVSENGSTVGGGAVSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTR+WHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRNWHRMVIERVRRLNEEKGYAVAIMMDT 180

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD
Sbjct: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSLNNLEEII+ASD
Sbjct: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLNNLEEIIVASD 360

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI
Sbjct: 361 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS
Sbjct: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL
Sbjct: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 594

BLAST of CmaCh17G001980 vs. NCBI nr
Match: KAG6574952.1 (hypothetical protein SDJN03_25591, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 585/594 (98.48%), Postives = 590/594 (99.33%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTSATRSPGISLSKHSLSKPSPSTAAFRFPLSF KSSIRASSSPDSNPLSSAF
Sbjct: 168 MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFPKSSIRASSSPDSNPLSSAF 227

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGST GGGALSSSVTREFVPV++DSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 228 TSQVLVSENGSTVGGGALSSSVTREFVPVITDSTAIEVDTVTEAELKENGFRSTRRTKLV 287

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 288 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 347

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNY GFAEDVRVGDDLLVD
Sbjct: 348 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYGGFAEDVRVGDDLLVD 407

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 408 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 467

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSLNNLEEII+ASD
Sbjct: 468 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLNNLEEIIVASD 527

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI
Sbjct: 528 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 587

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS
Sbjct: 588 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 647

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL
Sbjct: 648 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 707

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 708 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 761

BLAST of CmaCh17G001980 vs. NCBI nr
Match: KAG7013517.1 (hypothetical protein SDJN02_23683, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 585/594 (98.48%), Postives = 590/594 (99.33%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFTKSSIRASSSPDSNPLSSAF 60
           MTQSLQ+FTSATRSPGISLSKHSLSKPSPSTAAFRFPLSF KSSIRASSSPDSNPLSSAF
Sbjct: 126 MTQSLQLFTSATRSPGISLSKHSLSKPSPSTAAFRFPLSFPKSSIRASSSPDSNPLSSAF 185

Query: 61  TSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRSTRRTKLV 120
           TSQVLVSENGST GGGALSSSVTREFVPV++DSTAIEVDTVTEAELKENGFRSTRRTKLV
Sbjct: 186 TSQVLVSENGSTVGGGALSSSVTREFVPVITDSTAIEVDTVTEAELKENGFRSTRRTKLV 245

Query: 121 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 180
           CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT
Sbjct: 246 CTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVAIMMDT 305

Query: 181 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGDDLLVD 240
           EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNY GFAEDVRVGDDLLVD
Sbjct: 306 EGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYGGFAEDVRVGDDLLVD 365

Query: 241 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 300
           GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID
Sbjct: 366 GGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSKDWLDID 425

Query: 301 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEEIILASD 360
           FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISI+AKIESLDSLNNLEEII+ASD
Sbjct: 426 FGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISIIAKIESLDSLNNLEEIIVASD 485

Query: 361 GAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 420
           GAMVARGDLGAQIPLEQVP VQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI
Sbjct: 486 GAMVARGDLGAQIPLEQVPSVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTRAEVADI 545

Query: 421 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 480
           SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS
Sbjct: 546 SEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPEVGSSSS 605

Query: 481 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 540
           DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL
Sbjct: 606 DSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSVRRRLNL 665

Query: 541 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 666 QWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 719

BLAST of CmaCh17G001980 vs. TAIR 10
Match: AT3G22960.1 (Pyruvate kinase family protein )

HSP 1 Score: 910.2 bits (2351), Expect = 8.6e-265
Identity = 474/600 (79.00%), Postives = 528/600 (88.00%), Query Frame = 0

Query: 1   MTQSLQIFTSATRSPGISLSKHS-LSKPSPSTAAFRFPLSFTK-SSIRASS----SPDSN 60
           M+QS+Q F++ + +P +    HS  ++P  S +  RFPL+  K +SIRASS    SPD +
Sbjct: 1   MSQSIQ-FSTPSHTPHLLHLPHSQFNRPLSSISFRRFPLTTIKYTSIRASSSSSPSPDLD 60

Query: 61  PLSSAFTSQVLVSENGSTGGGGALSSSVTREFVPVMSDSTAIEVDTVTEAELKENGFRST 120
             SS+ +SQVL+S NG+   G   S   +     V +D++ IEVDTVTEAELKENGFRST
Sbjct: 61  SSSSSSSSQVLLSPNGT---GAVKSDERSVVATAVTTDTSGIEVDTVTEAELKENGFRST 120

Query: 121 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAV 180
           RRTKL+CTIGP TCGFEQLEALAVGGMNVAR+NMCHGTRDWHR VI  VRRLNEEKG+AV
Sbjct: 121 RRTKLICTIGPATCGFEQLEALAVGGMNVARLNMCHGTRDWHRGVIRSVRRLNEEKGFAV 180

Query: 181 AIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVG 240
           AIMMDTEGSEIHMGDLGG +SAKAEDGE+WTF+VRAFDS+ PERTI+V+Y+GFAEDVRVG
Sbjct: 181 AIMMDTEGSEIHMGDLGGEASAKAEDGEVWTFTVRAFDSSRPERTISVSYDGFAEDVRVG 240

Query: 241 DDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISSK 300
           D+LLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDG LVRERNAMLPTISSK
Sbjct: 241 DELLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGSLVRERNAMLPTISSK 300

Query: 301 DWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLEE 360
           DWLDIDFGIAEGVDF+A+SFVKSAEVI HLKSY+AARSRG +I ++AKIES+DSL NLEE
Sbjct: 301 DWLDIDFGIAEGVDFIAVSFVKSAEVINHLKSYLAARSRGGEIGVIAKIESIDSLTNLEE 360

Query: 361 IILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPTR 420
           IILASDGAMVARGDLGAQIPLEQVP  QQ++VQ+CR LNKPVIVASQLLESMIEYPTPTR
Sbjct: 361 IILASDGAMVARGDLGAQIPLEQVPAAQQRIVQVCRALNKPVIVASQLLESMIEYPTPTR 420

Query: 421 AEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELPE 480
           AEVAD+SEAVRQRSDALMLSGESAMGQ+PDKAL VLR VSLRIE+WWREEKRHE + L  
Sbjct: 421 AEVADVSEAVRQRSDALMLSGESAMGQFPDKALTVLRTVSLRIERWWREEKRHESVPLQA 480

Query: 481 VGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTSV 540
           +GSS SD I EEICNSAAKMANNL VDA+FVYT +GHMASL+SRCRPDCPIFAFT TTSV
Sbjct: 481 IGSSFSDKISEEICNSAAKMANNLGVDAVFVYTTSGHMASLVSRCRPDCPIFAFTTTTSV 540

Query: 541 RRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSIQVMNVP 595
           RRRLNLQWGLIPFR+SFSDDME+NLNK F LLK+R +IKSGDLVIAVSDMLQSIQVMNVP
Sbjct: 541 RRRLNLQWGLIPFRLSFSDDMESNLNKTFSLLKSRGMIKSGDLVIAVSDMLQSIQVMNVP 596

BLAST of CmaCh17G001980 vs. TAIR 10
Match: AT5G52920.1 (plastidic pyruvate kinase beta subunit 1 )

HSP 1 Score: 362.8 bits (930), Expect = 5.1e-100
Identity = 208/475 (43.79%), Postives = 290/475 (61.05%), Query Frame = 0

Query: 115 RRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLN-EEKGYA 174
           R+TK+VCT+GP T   E +  LA  GMNVAR+NM HG    H+ VI+ V+  N + K   
Sbjct: 110 RKTKIVCTVGPSTNTREMIWKLAEAGMNVARMNMSHGDHASHKKVIDLVKEYNAQTKDNT 169

Query: 175 VAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRV 234
           +AIM+DT+G E+  GDL        + G+ +TF++    ST     ++VNY+ F  DV  
Sbjct: 170 IAIMLDTKGPEVRSGDL--PQPIMLDPGQEFTFTIERGVST--PSCVSVNYDDFVNDVEA 229

Query: 235 GDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 294
           GD LLVDGGM+ F V  K    VKC   D G L  R +L        VR ++A LP+I+ 
Sbjct: 230 GDMLLVDGGMMSFMVKSKTKDSVKCEVVDGGELKSRRHLN-------VRGKSATLPSITE 289

Query: 295 KDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNLE 354
           KDW DI FG+   VDF A+SFVK A+V+  LK Y+  ++ G+DI ++ KIES DS+ NL 
Sbjct: 290 KDWEDIKFGVENKVDFYAVSFVKDAQVVHELKKYL--QNSGADIHVIVKIESADSIPNLH 349

Query: 355 EIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTPT 414
            II ASDGAMVARGDLGA++P+E+VP++Q++++ LCR + K VIVA+ +LESMI +PTPT
Sbjct: 350 SIITASDGAMVARGDLGAELPIEEVPILQEEIINLCRSMGKAVIVATNMLESMIVHPTPT 409

Query: 415 RAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWREEKRHEPMELP 474
           RAEV+DI+ AVR+ +DA+MLSGE+A G++P KA  V+  V+LR E      +       P
Sbjct: 410 RAEVSDIAIAVREGADAVMLSGETAHGKFPLKAAGVMHTVALRTEATITSGEMP-----P 469

Query: 475 EVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIFAFTETTS 534
            +G +  + + E     A  M+N L    + V+T+TG MA LLS  RP   I+AFT    
Sbjct: 470 NLGQAFKNHMSEMFAYHATMMSNTLGTSTV-VFTRTGFMAILLSHYRPSGTIYAFTNEKK 529

Query: 535 VRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDLVIAVSDMLQSI 589
           +++RL L  G+ P  M F+DD E         L  + ++K G+ +  V    Q I
Sbjct: 530 IQQRLALYQGVCPIYMEFTDDAEETFANALATLLKQGMVKKGEEIAIVQSGTQPI 565

BLAST of CmaCh17G001980 vs. TAIR 10
Match: AT1G32440.1 (plastidial pyruvate kinase 3 )

HSP 1 Score: 362.5 bits (929), Expect = 6.7e-100
Identity = 215/491 (43.79%), Postives = 303/491 (61.71%), Query Frame = 0

Query: 104 AELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERV 163
           A+ + +   S R+TK+VCTIGP +   E +  LA  GMNVAR+NM HG    H++ I+ V
Sbjct: 88  ADSRTSSNDSRRKTKIVCTIGPSSSSREMIWKLAEAGMNVARLNMSHGDHASHQITIDLV 147

Query: 164 RRLNE-EKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINV 223
           +  N      A+AIM+DT+G E+  GD+        E+G+ + F+++   S   + T++V
Sbjct: 148 KEYNSLFVDKAIAIMLDTKGPEVRSGDV--PQPIFLEEGQEFNFTIKRGVSL--KDTVSV 207

Query: 224 NYEGFAEDVRVGDDLLVDGGMVRFEVIEKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVR 283
           NY+ F  DV VGD LLVDGGM+   V  K    VKC+  D G L  R +L        VR
Sbjct: 208 NYDDFVNDVEVGDILLVDGGMMSLAVKSKTSDLVKCVVIDGGELQSRRHLN-------VR 267

Query: 284 ERNAMLPTISSKDWLDIDFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAK 343
            ++A LP+I+ KDW DI FG+   VDF A+SFVK A+V+  LK+Y+  ++  +DIS++ K
Sbjct: 268 GKSATLPSITDKDWEDIKFGVDNQVDFYAVSFVKDAKVVHELKNYL--KTCSADISVIVK 327

Query: 344 IESLDSLNNLEEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQL 403
           IES DS+ NL  II A DGAMVARGDLGA++P+E+VPL+Q+++++ CR ++KPVIVA+ +
Sbjct: 328 IESADSIKNLPSIISACDGAMVARGDLGAELPIEEVPLLQEEIIRRCRSIHKPVIVATNM 387

Query: 404 LESMIEYPTPTRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEKWWR 463
           LESMI +PTPTRAEV+DI+ AVR+ +DA+MLSGE+A G++P KA+ V+  V+LR E    
Sbjct: 388 LESMINHPTPTRAEVSDIAIAVREGADAIMLSGETAHGKFPLKAVNVMHTVALRTE---- 447

Query: 464 EEKRHEPMELPEVGSSSSDSIL-----EEICNSAAKMANNLEVDAIFVYTKTGHMASLLS 523
                    LP   S+S  +       +     A+ MAN L    + V+T+TG MA LLS
Sbjct: 448 -------ASLPVRTSASRTTAYKGHMGQMFAFHASIMANTLS-SPLIVFTRTGSMAVLLS 507

Query: 524 RCRPDCPIFAFTETTSVRRRLNLQWGLIPFRMSFSDDMENNLNKIFLLLKARNLIKSGDL 583
             RP   IFAFT    + +RL L  G++P  M FSDD E+   +   LL+  N++K G  
Sbjct: 508 HYRPSATIFAFTNQRRIMQRLALYQGVMPIYMEFSDDAEDTYARSLKLLQDENMLKEGQH 553

Query: 584 VIAVSDMLQSI 589
           V  V    Q I
Sbjct: 568 VTLVQSGSQPI 553

BLAST of CmaCh17G001980 vs. TAIR 10
Match: AT5G63680.1 (Pyruvate kinase family protein )

HSP 1 Score: 239.2 bits (609), Expect = 8.5e-63
Identity = 158/505 (31.29%), Postives = 263/505 (52.08%), Query Frame = 0

Query: 105 ELKENGFRSTRRTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVR 164
           EL  +G   T +TK+VCT+GP +     +E L   GMNVAR N  HG+ ++H+  ++ +R
Sbjct: 12  ELPNDG--RTPKTKIVCTLGPASRSVTMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLR 71

Query: 165 RLNEEKGYAVAIMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNY 224
              +  G   A+M+DT+G EI  G L   +  + ++G+  T +   +D    E+TI+++Y
Sbjct: 72  TAMQNTGILAAVMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDIKGDEKTISMSY 131

Query: 225 EGFAEDVRVGDDLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVR 284
           +    DV+ G+ +L   G +   V+  +     V C C +  +L  R N+     G +V 
Sbjct: 132 KKLPVDVKPGNTILCADGSISLAVVSCDPNAGTVICRCENTAMLGERKNVNL--PGVVVD 191

Query: 285 ERNAMLPTISSKDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILA 344
                LPT++ KD  DI  +G+   +D +A+SFV+    + +++  + + S+   I +++
Sbjct: 192 -----LPTLTDKDVEDILKWGVPNNIDMIALSFVRKGSDLVNVRKVLGSHSK--SIMLMS 251

Query: 345 KIESLDSLNNLEEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQ 404
           K+E+ + + N +EI+  +D  MVARGDLG +IP+E++ L Q+ ++  C    KPV+ A+Q
Sbjct: 252 KVENQEGVLNFDEILRETDAFMVARGDLGMEIPIEKIFLAQKMMIYKCNLAGKPVVTATQ 311

Query: 405 LLESMIEYPTPTRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEK-- 464
           +LESMI+ P PTRAE  D++ AV   +D +MLSGESA G YP+ A+  +  + +  E   
Sbjct: 312 MLESMIKSPRPTRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKTMAKICIEAESSL 371

Query: 465 ----WWREEKRHEPMELPEVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASL 524
                ++E  R  P+ +         S LE + +SA + AN  +   I V T+ G  A L
Sbjct: 372 DYNTIFKEMIRATPLPM---------STLESLASSAVRTANKAKAKLIIVLTRGGTTAKL 431

Query: 525 LSRCRPDCPIFA-----FTETT--------SVRRRLNLQWGLIPF------RMSFSDDME 582
           +++ RP  PI +     FT  T        S  R   +  GLIP       + + S+  E
Sbjct: 432 VAKYRPAVPILSVVVPVFTSDTFNWSCSDESPARHSLIYRGLIPVLGEGSAKATDSESTE 491

BLAST of CmaCh17G001980 vs. TAIR 10
Match: AT5G08570.1 (Pyruvate kinase family protein )

HSP 1 Score: 233.0 bits (593), Expect = 6.1e-61
Identity = 139/421 (33.02%), Postives = 232/421 (55.11%), Query Frame = 0

Query: 116 RTKLVCTIGPVTCGFEQLEALAVGGMNVARINMCHGTRDWHRMVIERVRRLNEEKGYAVA 175
           +TK+VCT+GP +     +E L   GMNVAR N  HG+ ++H+  ++ +R      G   A
Sbjct: 21  KTKIVCTLGPASRTVSMIEKLLKAGMNVARFNFSHGSHEYHQETLDNLRSAMHNTGILAA 80

Query: 176 IMMDTEGSEIHMGDLGGASSAKAEDGEIWTFSVRAFDSTLPERTINVNYEGFAEDVRVGD 235
           +M+DT+G EI  G L   +  + ++G+  T +   +D    E TI+++Y+    DV+ G+
Sbjct: 81  VMLDTKGPEIRTGFLKDGNPIQLKEGQEITITT-DYDIQGDESTISMSYKKLPLDVKPGN 140

Query: 236 DLLVDGGMVRFEVI--EKIGPDVKCLCTDPGLLLPRANLTFWRDGHLVRERNAMLPTISS 295
            +L   G +   V+  +     V+C C +  +L  R N+     G +V      LPT++ 
Sbjct: 141 TILCADGSISLAVLSCDPESGTVRCRCENSAMLGERKNVNL--PGVVVD-----LPTLTD 200

Query: 296 KDWLDI-DFGIAEGVDFLAISFVKSAEVIKHLKSYIAARSRGSDISILAKIESLDSLNNL 355
           KD  DI  +G+   +D +A+SFV+    + +++  +   S    I +++K+E+ + + N 
Sbjct: 201 KDIEDILGWGVPNSIDMIALSFVRKGSDLVNVRKVLG--SHAKSIMLMSKVENQEGVINF 260

Query: 356 EEIILASDGAMVARGDLGAQIPLEQVPLVQQKVVQLCRQLNKPVIVASQLLESMIEYPTP 415
           +EI+  +D  MVARGDLG +IP+E++ L Q+ ++  C    KPV+ A+Q+LESMI+ P P
Sbjct: 261 DEILRETDAFMVARGDLGMEIPIEKIFLAQKLMIYKCNLAGKPVVTATQMLESMIKSPRP 320

Query: 416 TRAEVADISEAVRQRSDALMLSGESAMGQYPDKALAVLRNVSLRIEK------WWREEKR 475
           TRAE  D++ AV   +D +MLSGESA G YP+ A+ V+  + +  E        ++E  R
Sbjct: 321 TRAEATDVANAVLDGTDCVMLSGESAAGAYPEIAVKVMAKICIEAESSLDYNTIFKEMIR 380

Query: 476 HEPMELPEVGSSSSDSILEEICNSAAKMANNLEVDAIFVYTKTGHMASLLSRCRPDCPIF 528
             P+ +         S LE + +SA + AN      I V T+ G  A+L+++ RP  PI 
Sbjct: 381 ATPLPM---------SPLESLASSAVRTANKARAKLIIVLTRGGSTANLVAKYRPAVPIL 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q431176.4e-26580.57Pyruvate kinase isozyme A, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1[more]
Q9LIK01.2e-26379.00Plastidial pyruvate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=P... [more]
Q405451.0e-26278.97Pyruvate kinase isozyme A, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q405461.3e-10044.65Pyruvate kinase isozyme G, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9FLW97.2e-9943.79Plastidial pyruvate kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PKP2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1L0W90.0e+00100.00Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1[more]
A0A6J1KWK20.0e+0099.66Pyruvate kinase OS=Cucurbita maxima OX=3661 GN=LOC111499365 PE=3 SV=1[more]
A0A6J1H3D90.0e+0097.99Pyruvate kinase OS=Cucurbita moschata OX=3662 GN=LOC111460037 PE=3 SV=1[more]
A0A5A7UTK22.0e-30692.76Pyruvate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G0015... [more]
A0A1S3BLQ92.0e-30692.76Pyruvate kinase OS=Cucumis melo OX=3656 GN=LOC103491219 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023006710.10.0e+00100.00pyruvate kinase isozyme A, chloroplastic-like isoform X2 [Cucurbita maxima][more]
XP_023006707.10.0e+0099.66pyruvate kinase isozyme A, chloroplastic-like isoform X1 [Cucurbita maxima][more]
XP_023548664.10.0e+0098.82pyruvate kinase isozyme A, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG6574952.10.0e+0098.48hypothetical protein SDJN03_25591, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7013517.10.0e+0098.48hypothetical protein SDJN02_23683, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G22960.18.6e-26579.00Pyruvate kinase family protein [more]
AT5G52920.15.1e-10043.79plastidic pyruvate kinase beta subunit 1 [more]
AT1G32440.16.7e-10043.79plastidial pyruvate kinase 3 [more]
AT5G63680.18.5e-6331.29Pyruvate kinase family protein [more]
AT5G08570.16.1e-6133.02Pyruvate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001697Pyruvate kinasePRINTSPR01050PYRUVTKNASEcoord: 307..321
score: 41.74
coord: 366..390
score: 47.82
coord: 416..434
score: 46.56
coord: 391..415
score: 60.31
coord: 339..365
score: 49.17
coord: 435..451
score: 41.81
coord: 172..188
score: 45.07
IPR001697Pyruvate kinaseTIGRFAMTIGR01064TIGR01064coord: 115..583
e-value: 9.4E-128
score: 424.6
IPR001697Pyruvate kinasePANTHERPTHR11817PYRUVATE KINASEcoord: 83..594
IPR015795Pyruvate kinase, C-terminalPFAMPF02887PK_Ccoord: 485..584
e-value: 4.9E-23
score: 81.6
IPR040442Pyruvate kinase-like domain superfamilyGENE3D3.20.20.60coord: 115..457
e-value: 6.0E-150
score: 501.8
IPR036918Pyruvate kinase, C-terminal domain superfamilyGENE3D3.40.1380.20coord: 458..584
e-value: 6.0E-150
score: 501.8
IPR036918Pyruvate kinase, C-terminal domain superfamilySUPERFAMILY52935PK C-terminal domain-likecoord: 446..592
IPR015806Pyruvate kinase, insert domain superfamilyGENE3D2.40.33.10coord: 183..289
e-value: 6.0E-150
score: 501.8
IPR015793Pyruvate kinase, barrelPFAMPF00224PKcoord: 115..462
e-value: 6.7E-91
score: 304.8
NoneNo IPR availablePANTHERPTHR11817:SF14PLASTIDIAL PYRUVATE KINASE 1, CHLOROPLASTICcoord: 83..594
IPR018209Pyruvate kinase, active sitePROSITEPS00110PYRUVATE_KINASEcoord: 337..349
IPR011037Pyruvate kinase-like, insert domain superfamilySUPERFAMILY50800PK beta-barrel domain-likecoord: 184..275
IPR015813Pyruvate/Phosphoenolpyruvate kinase-like domain superfamilySUPERFAMILY51621Phosphoenolpyruvate/pyruvate domaincoord: 96..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G001980.1CmaCh17G001980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0010431 seed maturation
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030955 potassium ion binding
molecular_function GO:0004743 pyruvate kinase activity
molecular_function GO:0003824 catalytic activity