CmaCh17G000330 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh17G000330
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionvestitone reductase-like
LocationCma_Chr17: 145366 .. 148708 (-)
RNA-Seq ExpressionCmaCh17G000330
SyntenyCmaCh17G000330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTTATTGGTAGAAGCGAGAATGAGAAATTCTCCATTATGATAGAAAGTTGGGTAAAATCCCAATATTTCTAAAAAGTGTGATGAACACGTGAAATGGGTATAATTTATTTCCTGATATAAACAATTGCGTGGGATTGCAATCTTTACAAATCGTTTCTGGAAAGGGCAGAAATCGAGAGAAATGGAAAATGGGGCAAAGGGGAAGGTGTGTGTAACAGGAGGCACTGGATTTATAGCGTCATGGCTGATCAAGAGCCTTCTCGAGAATGGCTACTCTGTAACCACCACTGTTAGAGCCGACCCAGGTAAAGCAAGACCCCTTTCTCCTTTCCATTTTTGGTCTCTGTTTTGATTTCTTGCCTTTGCCTGGTTGACTTCCAGATCAGCTTCTTGGATTGTAAAGGATACTCTCCTTCCAATTCTGTACTTGAGTCATTTGAATGTTATATGAATTCTTTACTGGATTTCACTTTTTTTTACTTCTTTTGAGGTTGCATGCTAGGTGTTTGATGATATTACTATGAGAGTTGATCACTTGACTGCTATTTCAGCGCAGTGCCTTTGGCTCTTTAGATCCATATTAAGCTGCCGCGAAGTTCAAGTGGATTGGTTACTGTATTATTCCCTTGCCATAGAAGACTCGGTGTTAATAATTTTGTTTCTTCTTTCTGCTTTTAAGTTTTTGGAATTCATTAAGGATTGTAAGAAGTAGATCCAACCACAAATGAAGTTTTCATTGAACAAGTAAGAATTTATAAAACAAACCACCAATAACTAAATCGTTATTGATTATATTTTAAGAGGTATAAGCTTGTGCAATACTTTTCTAACTAGTTTTCTGTATGTAAACTTGTTTACATTTTAATTTCCAAATAGATCAATTGTGTAACTCGTCTTTCCTCATCTAAATTTGTTCACATTTTAATACGGCACAGAGATGAGAAGGGACTATGGATTCTTGACAAATCTACCCGGAGCATCAGAAAAACTTCAAATATACCATGCTGATCTAGACGACCCGAATAGTTTTGCTCCTGCCATTGCAGGTTGCATTGGGGTCTTTCATCTTGCTACCCCAATTGATATGAGTGACAAAGAAGGTGTGGAAGTGTTGACTAGAAGAACGATTGAAGGAACATTGGGCATCCTTAAAGTTTGTCTAGATTCAAAAACGGTAAGGCGAGTTGTATACACTTCCGCTGCAGCCACCATGCAGTTTAATCACCACACAGTAGACTTTTTGGATGAAAGTTGTTGGAGTGACATTGATTACATCAATAGTATTGCACCACTTGGAAGATCATACCCGATTTCTAAGACATTAACTGAGCAAGCAGTTCTTGAGTTCTCTCAACAATACGGATTGGAAGTTGTCACTGTCCTTCCAACTTATGTTGTTGGTCCCTTCATTTGTCCTAGACTTCCCGACTCTATTCGTGTGACAACGTCTCTGATGTCAGGTACTTTTTTTTTAATGTTTAAAGTATCTTATGCTTGTTGAATGCATCTTGCACTTTGCACGTCGACTTGGAATCTTCATTGAAGAGTAGGCAGATGCCTAAGTTTTACTGAATCTTATTGCTAGAGACATATTTGGCAGTTGATCTTTATAAAATTATATATTGTTTTTGAACTTTTGAGCATTGTTTCCTTTTTAATCCTCAACCTCTTATAGGGTCCAATTACAGAACATTTGAATTACGTCTCAGAAGTTAAACATTATGTTTTTGTTTAGTGCAAGCCATTAATACGAATGATTGAATGTTATTATGACATGCCCATTTGATCGATTTTGATTGAATTGGCTGAAGTAACATATAGTTGAACAAGTTAATGAGCATGCAATGGCATGCATTATTTTGGGGATGGTATAAATGATAAGGACTACATAGACTGTAATTCAATTTGAACTTTTGAAGACATTTCCTATCTTCATTTTGCTTGGGATACCTCTTTTATCTCTGTTCTTTGACAATAGCTATAATGATATTATGAGTTGACTGAGTTCATGATCCATAGACTCCATGGAATTATGCTCTAATTTAGGGCCATTATGGATTGACTTTCGTAGTGTTCTAAACACTTGTTTTACACTTAACAAAGCTTTTGTAAGCACTTAGAAAGTCAATCCAAATAGGCCGTTAAGCTAGGAAGTTGTGGGATGCTTAAATATTTTATTCCCTTGCTGAATTTCAAACTTGAGTAGCATCAATGTGAGCCCTTTAGCTTCTTTTGCATGGAAATTAGTAAATTACACTCTGTTTATGACTTAGTTCCAAAATGATATTAAACATGAAAATTGTGCAGGTAATAAAGCAGAGTATGGATTGATCTTAAAATCAAACATGGTGCATGTAGATGACGTGGCAAGATCACACATTTTTCTCTTTGAACATCCAAATGCAAATGGGCGATATGTTTGCTCTTCCCACTTAATCACAATCGAAGAATTGGCTAATCTCTTTTCTGCCAAGTACCCAGAATTTCAGATACCATCGCCTGAGTGAGTTCCACTGCATATTAGTTTCCCATTATCATTACAGAATCTTCTCTCTTAGTAATATTGCAAAGTTAGCCTTCGATAATCATTTCATTTTTTATTTTTGGTTTTTTGAAAATTAGGTTTAAAAACATTACTTCTACTTATTGATTTTGTTATTTTATTGTCCTCTTTTTAGACATGTTTTCAAAATCCAATCCAAGTATTGAAAACTAAAAAATAGTTCTAAAAAACTTGATTTTTTTTTTTTTTGTTTGTTTGGAATTTGGCTATCAAATGGGGTCTTAACAAGGTTAATGATCGTGGTGTTTATTTATAATTTTTTTTTTTGAATTTGTGATTCTATGTAGCCCTTCTGTATATGCTTATTGGATAAATATTGATTGGTTTGGCCCAATTTGATTGGTAGATGATAGAAGAAAGGTGATACTCAGTCAAAATGAAGTTTGGCATACAATATGCCCCTATTTTGAAACAACAAATCAAATCTCTTTTATAGACCTTTTTGTTCAACTGTTCACTTCTTTTTTATCATTTATTTATCAAATCATGCAAGATTTTTCCATTAAGCATATTACATCGTCATCATTATCAATTTCACAACAGGGACTTTAAAGGTTCGAAGTAAGACAAAGTTGTTTATTTGAAGTTTGGGGATTAAATAAATACAACTGATTCGACTGCAGGTCTTTGAAAGATGTCAAAGGCTACATATTCAGTGATGTCTCTTCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAATATGGTATTGAAGAAATGTTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCCATGAAACAAATTAG

mRNA sequence

CTTTTTATTGGTAGAAGCGAGAATGAGAAATTCTCCATTATGATAGAAAGTTGGGTAAAATCCCAATATTTCTAAAAAGTGTGATGAACACGTGAAATGGGTATAATTTATTTCCTGATATAAACAATTGCGTGGGATTGCAATCTTTACAAATCGTTTCTGGAAAGGGCAGAAATCGAGAGAAATGGAAAATGGGGCAAAGGGGAAGGTGTGTGTAACAGGAGGCACTGGATTTATAGCGTCATGGCTGATCAAGAGCCTTCTCGAGAATGGCTACTCTGTAACCACCACTGTTAGAGCCGACCCAGAGATGAGAAGGGACTATGGATTCTTGACAAATCTACCCGGAGCATCAGAAAAACTTCAAATATACCATGCTGATCTAGACGACCCGAATAGTTTTGCTCCTGCCATTGCAGGTTGCATTGGGGTCTTTCATCTTGCTACCCCAATTGATATGAGTGACAAAGAAGGTGTGGAAGTGTTGACTAGAAGAACGATTGAAGGAACATTGGGCATCCTTAAAGTTTGTCTAGATTCAAAAACGGTAAGGCGAGTTGTATACACTTCCGCTGCAGCCACCATGCAGTTTAATCACCACACAGTAGACTTTTTGGATGAAAGTTGTTGGAGTGACATTGATTACATCAATAGTATTGCACCACTTGGAAGATCATACCCGATTTCTAAGACATTAACTGAGCAAGCAGTTCTTGAGTTCTCTCAACAATACGGATTGGAAGTTGTCACTGTCCTTCCAACTTATGTTGTTGGTCCCTTCATTTGTCCTAGACTTCCCGACTCTATTCGTGTGACAACGTCTCTGATGTCAGGTAATAAAGCAGAGTATGGATTGATCTTAAAATCAAACATGGTGCATGTAGATGACGTGGCAAGATCACACATTTTTCTCTTTGAACATCCAAATGCAAATGGGCGATATGTTTGCTCTTCCCACTTAATCACAATCGAAGAATTGGCTAATCTCTTTTCTGCCAAGTACCCAGAATTTCAGATACCATCGCCTGAGTCTTTGAAAGATGTCAAAGGCTACATATTCAGTGATGTCTCTTCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAATATGGTATTGAAGAAATGTTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCCATGAAACAAATTAG

Coding sequence (CDS)

ATGGAAAATGGGGCAAAGGGGAAGGTGTGTGTAACAGGAGGCACTGGATTTATAGCGTCATGGCTGATCAAGAGCCTTCTCGAGAATGGCTACTCTGTAACCACCACTGTTAGAGCCGACCCAGAGATGAGAAGGGACTATGGATTCTTGACAAATCTACCCGGAGCATCAGAAAAACTTCAAATATACCATGCTGATCTAGACGACCCGAATAGTTTTGCTCCTGCCATTGCAGGTTGCATTGGGGTCTTTCATCTTGCTACCCCAATTGATATGAGTGACAAAGAAGGTGTGGAAGTGTTGACTAGAAGAACGATTGAAGGAACATTGGGCATCCTTAAAGTTTGTCTAGATTCAAAAACGGTAAGGCGAGTTGTATACACTTCCGCTGCAGCCACCATGCAGTTTAATCACCACACAGTAGACTTTTTGGATGAAAGTTGTTGGAGTGACATTGATTACATCAATAGTATTGCACCACTTGGAAGATCATACCCGATTTCTAAGACATTAACTGAGCAAGCAGTTCTTGAGTTCTCTCAACAATACGGATTGGAAGTTGTCACTGTCCTTCCAACTTATGTTGTTGGTCCCTTCATTTGTCCTAGACTTCCCGACTCTATTCGTGTGACAACGTCTCTGATGTCAGGTAATAAAGCAGAGTATGGATTGATCTTAAAATCAAACATGGTGCATGTAGATGACGTGGCAAGATCACACATTTTTCTCTTTGAACATCCAAATGCAAATGGGCGATATGTTTGCTCTTCCCACTTAATCACAATCGAAGAATTGGCTAATCTCTTTTCTGCCAAGTACCCAGAATTTCAGATACCATCGCCTGAGTCTTTGAAAGATGTCAAAGGCTACATATTCAGTGATGTCTCTTCAAAGAAGCTTTTGGATGCTGGTTTTCAATACAAATATGGTATTGAAGAAATGTTTGATGGAGCAATCCAAAGCTGCAAAGAGAAGGGTTATCTCCATGAAACAAATTAG

Protein sequence

MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSHIFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKLLDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Homology
BLAST of CmaCh17G000330 vs. ExPASy Swiss-Prot
Match: Q40316 (Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 3.5e-111
Identity = 186/323 (57.59%), Postives = 249/323 (77.09%), Query Frame = 0

Query: 6   KGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHA 65
           KG+VCVTGGTGF+ SW+IKSLLENGYSV TT+RADPE +RD  FLTNLPGASEKL  ++A
Sbjct: 5   KGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERKRDVSFLTNLPGASEKLHFFNA 64

Query: 66  DLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRV 125
           DL +P+SFA AI GC+G+FH A+PID +  E  E++T+RT++G LGILK C++SKTV+R 
Sbjct: 65  DLSNPDSFAAAIEGCVGIFHTASPIDFAVSEPEEIVTKRTVDGALGILKACVNSKTVKRF 124

Query: 126 VYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQQYGL 185
           +YTS+ + + FN    D LDES WSD+D + S+ P G +Y +SKTL E+AVLEF +Q G+
Sbjct: 125 IYTSSGSAVSFNGKDKDVLDESDWSDVDLLRSVKPFGWNYAVSKTLAEKAVLEFGEQNGI 184

Query: 186 EVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSHIFLFE 245
           +VVT++  ++VG F+CP+LPDSI     L+ G K + G + + +MVHVDDVAR+HI+L E
Sbjct: 185 DVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIG-VTRFHMVHVDDVARAHIYLLE 244

Query: 246 HPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKLLDAGF 305
           +    GRY CS  ++ IEE++ L SAKYPE+QI + + LK++KG    D+++KKL+DAGF
Sbjct: 245 NSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQILTVDELKEIKGARLPDLNTKKLVDAGF 304

Query: 306 QYKYGIEEMFDGAIQSCKEKGYL 329
            +KY IE+MFD AIQ CKEKGYL
Sbjct: 305 DFKYTIEDMFDDAIQCCKEKGYL 326

BLAST of CmaCh17G000330 vs. ExPASy Swiss-Prot
Match: O22133 (Protein BRI1-5 ENHANCED 1 OS=Arabidopsis thaliana OX=3702 GN=BEN1 PE=2 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 7.6e-82
Identity = 162/328 (49.39%), Postives = 219/328 (66.77%), Query Frame = 0

Query: 7   GKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPE-MRRDYGFLTNLPGASEKLQIYHA 66
           G VCVTGG+GF+ASWLI  LL+ GYSV  TVR + E  ++D  +LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 67  DLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRV 126
           DL++P SF PAI GC  VFH+A P+D +  E  E +T+RT++G +GILK CLD+KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 127 VYTSAAATMQF---NHHTVDFLDESCWSDIDYINSIAP--LGRSYPISKTLTEQAVLEFS 186
            YTS+A T+ +   N      +DES WSD++   +     +  SY +SK   E A LEF 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 187 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 246
            + GLEVVT++   VVGPFI   LP S+ ++ +++ GN  E  L    NMVH+DDVAR+ 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 247 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 306
           IFL E P A GRY+CSS  + I+E+    S K+P+FQ+PS + L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 307 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
             AGF++KYG EE+F GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

BLAST of CmaCh17G000330 vs. ExPASy Swiss-Prot
Match: I3PLR3 (Noscapine synthase SDR1 OS=Papaver somniferum OX=3469 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.6e-79
Identity = 157/326 (48.16%), Postives = 217/326 (66.56%), Query Frame = 0

Query: 9   VCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHADLD 68
           VCVTGG G++ASWLI  LLE GYSV TTVR+DP+ R D   L  LP A+EKLQI+ ADL+
Sbjct: 24  VCVTGGAGYLASWLIMRLLERGYSVRTTVRSDPKFREDVSHLKALPEATEKLQIFEADLE 83

Query: 69  DPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRVVYT 128
           +P SF  AI GC+GVF +A  ++ +++  +E + +  +EGTL IL+ CL SKTV++VVYT
Sbjct: 84  NPESFDDAINGCVGVFLVAQGMNFAEEYTLEKIIKTCVEGTLRILQSCLKSKTVKKVVYT 143

Query: 129 SA--AATMQFNHHTVDFLDESCWSDIDYINS----IAPLGRSYPISKTLTEQAVLEFSQQ 188
           S+  AA M  N   V  +DE+ WS++D   S    + P   SY +SK LTE+A L+FS++
Sbjct: 144 SSADAAMMISNLKAVKEIDETIWSEVDNFISKPEQVIPGLPSYVVSKVLTERACLKFSEE 203

Query: 189 YGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSHIF 248
           +GL+VVT+LP  VVGPFI P  P S+ +  S++SG+ +    +   N VH+DDVA +HIF
Sbjct: 204 HGLDVVTILPPLVVGPFITPHPPPSVSIALSIISGDVSMMLGVRLENAVHIDDVALAHIF 263

Query: 249 LFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKLLD 308
           +FE   A GR++CSS    + +L    S  YPEF +P+ + LKD++      +SS KLL 
Sbjct: 264 VFECEKAKGRHICSSVDFPMHDLPKFISENYPEFNVPT-DLLKDIEEQEPVHLSSDKLLS 323

Query: 309 AGFQYKYGIEEMFDGAIQSCKEKGYL 329
            GFQ+KY   E+F  AI+  KEKG+L
Sbjct: 324 MGFQFKYDFAEIFGDAIRCAKEKGFL 348

BLAST of CmaCh17G000330 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.0e-78
Identity = 146/334 (43.71%), Postives = 217/334 (64.97%), Query Frame = 0

Query: 4   GAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIY 63
           G    VCVTG  GF+ASWL+K LLE GY V  TVR DPE +     L NLPGA+++L+++
Sbjct: 10  GQTTTVCVTGAAGFMASWLVKRLLEKGYIVHATVR-DPENKAKVSHLLNLPGATDRLKLF 69

Query: 64  HADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVR 123
            A+L +  SF  A+AGC GVFH+ATP +   K+    L +  IEGTL +LK C    +++
Sbjct: 70  RAELCEDGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIK 129

Query: 124 RVVYTSAAATMQFNHHTV--DFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQ 183
           RVV TS+AAT+  N+ +    ++DESCW+D++++ S  P G +YP+SKTL EQA L++++
Sbjct: 130 RVVVTSSAATVSINNSSEQNQYIDESCWTDVNFLTSQKPPGWAYPVSKTLAEQAALKYAE 189

Query: 184 QYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLIL-------KSNMVHVD 243
           ++ L+VVTV+P  VVGP + P +P S+ +  SL++G++ + G +          ++VH+D
Sbjct: 190 EHSLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGSISLVHID 249

Query: 244 DVARSHIFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSD 303
           DV  + IFL E P+A GRY+C      I +LA   S +YP++++P+     DV       
Sbjct: 250 DVCSAQIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQYKVPT--KFDDVPATPKLT 309

Query: 304 VSSKKLLDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           +SS+KLLD GF +KYGIE+++D AI+  K KG L
Sbjct: 310 ISSQKLLDCGFSFKYGIEDIYDQAIEYMKTKGLL 340

BLAST of CmaCh17G000330 vs. ExPASy Swiss-Prot
Match: Q9XES5 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica OX=3750 GN=DFR PE=1 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 5.1e-78
Identity = 143/325 (44.00%), Postives = 207/325 (63.69%), Query Frame = 0

Query: 9   VCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHADLD 68
           VCVTG +GFI SWL+  LLE+GY+V  TVR DP  ++    L +LP A   L ++ ADL 
Sbjct: 8   VCVTGASGFIGSWLVMRLLEHGYTVRATVR-DPTNQKKVKHLLDLPKAETHLTLWKADLA 67

Query: 69  DPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRVVYT 128
           D  SF  AI GC GVFH+ATP+D   K+    + + TI G L ILK C  +KTVR++V+T
Sbjct: 68  DEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFT 127

Query: 129 SAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQQYGLEVV 188
           S+A T+    H     DES WSD+++  S+   G  Y +SKTL EQA  +++++  ++ +
Sbjct: 128 SSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFI 187

Query: 189 TVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSHIFLFEHPN 248
           T++PT V+GPF+ P +P S+    S +  N++ YG+I +   VH+DD+  SHI+L+EHP 
Sbjct: 188 TIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYEHPK 247

Query: 249 ANGRYVCSSHLITIEELANLFSAKYPEFQIPS-----PESLKDVKGYIFSDVSSKKLLDA 308
           A GRY+CSSH  TI EL  +   KYPE+ IP+      ++L+ V        SSKKL + 
Sbjct: 248 AEGRYICSSHDATIHELVKMLREKYPEYNIPTKFKGIDDNLEPV------HFSSKKLREI 307

Query: 309 GFQYKYGIEEMFDGAIQSCKEKGYL 329
           GF++KY +E+MF GA+ +C+ KG +
Sbjct: 308 GFEFKYSLEDMFVGAVDACRAKGLI 325

BLAST of CmaCh17G000330 vs. ExPASy TrEMBL
Match: A0A6J1KZ90 (vestitone reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498944 PE=3 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 3.6e-191
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 333
           LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 332

BLAST of CmaCh17G000330 vs. ExPASy TrEMBL
Match: A0A6J1H5D7 (vestitone reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111460207 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.1e-184
Identity = 318/332 (95.78%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLE GYSVTTTVRADPEMR+DYGFLT+LPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTSLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLD+PNSFAPAIAGCIGVFHLATPID SDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAP GRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPFGRSYPISKTLTERAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKA+YGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSH+IT+EEL NLFSAKYPEFQIPS ESLKDVKGY+FSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 333
           LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 332

BLAST of CmaCh17G000330 vs. ExPASy TrEMBL
Match: A0A5D3DLI4 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00450 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.0e-167
Identity = 286/328 (87.20%), Postives = 310/328 (94.51%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLENGYSVTTTVRADPE R+DY FLTNLPGASEKL
Sbjct: 1   MESAAKGKVCVTGGTGFVASWLIKKLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIY ADL DPNSFAPAIAGCIGVFHLATPID+ DKE VE LTRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPVESLTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDE+CWSDIDYIN+IAPLGRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDENCWSDIDYINNIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN++EYGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNESEYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           I+LFEHP+A+GRYVCSSH+IT+EELAN FSAKYPEFQIPSPESLKDVKGYIF+DVSSKKL
Sbjct: 241 IYLFEHPSASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           LDAGFQYKYGIEEM DGAI+SCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMLDGAIESCKEKGYL 328

BLAST of CmaCh17G000330 vs. ExPASy TrEMBL
Match: A0A1S3CBT5 (vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103499024 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.0e-167
Identity = 286/328 (87.20%), Postives = 310/328 (94.51%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLENGYSVTTTVRADPE R+DY FLTNLPGASEKL
Sbjct: 1   MESAAKGKVCVTGGTGFVASWLIKKLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIY ADL DPNSFAPAIAGCIGVFHLATPID+ DKE VE LTRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPVESLTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDE+CWSDIDYIN+IAPLGRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDENCWSDIDYINNIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN++EYGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNESEYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           I+LFEHP+A+GRYVCSSH+IT+EELAN FSAKYPEFQIPSPESLKDVKGYIF+DVSSKKL
Sbjct: 241 IYLFEHPSASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           LDAGFQYKYGIEEM DGAI+SCKEKGYL
Sbjct: 301 LDAGFQYKYGIEEMLDGAIESCKEKGYL 328

BLAST of CmaCh17G000330 vs. ExPASy TrEMBL
Match: A0A0A0K9S9 (Cinnamoyl-CoA reductase 16 OS=Cucumis sativus OX=3659 GN=CCR16 PE=2 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.0e-166
Identity = 286/328 (87.20%), Postives = 309/328 (94.21%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           ME+ AKGKVCVTGGTGF+ASWLIK LLENGYSVTTTVRADPE R+DY FLTNLPGASEKL
Sbjct: 1   MESVAKGKVCVTGGTGFVASWLIKRLLENGYSVTTTVRADPEKRKDYSFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIY ADL DPNSFAPAIAGCIGVFHLATPID+ DKE +E +TRRTIEGTLGILK+ +DSK
Sbjct: 61  QIYQADLHDPNSFAPAIAGCIGVFHLATPIDVDDKEPLESVTRRTIEGTLGILKLSVDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTS+AATMQFNHH VDFLDESCWSDIDYINSIAPLGRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSSAATMQFNHHKVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEKAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICP++P S+ V  SL+ GN+ EYGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPKIPGSVHVILSLILGNETEYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           I+LFE+PNA+GRYVCSSH+IT+EELAN FSAKYPEFQIPSPESLKDVKGYIF+DVSSKKL
Sbjct: 241 IYLFENPNASGRYVCSSHIITLEELANFFSAKYPEFQIPSPESLKDVKGYIFTDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           LDAGFQYKYG+EEM DGAIQSCKEKGYL
Sbjct: 301 LDAGFQYKYGVEEMLDGAIQSCKEKGYL 328

BLAST of CmaCh17G000330 vs. NCBI nr
Match: XP_023006100.1 (vestitone reductase-like [Cucurbita maxima])

HSP 1 Score: 677.2 bits (1746), Expect = 7.3e-191
Identity = 332/332 (100.00%), Postives = 332/332 (100.00%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 333
           LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 332

BLAST of CmaCh17G000330 vs. NCBI nr
Match: XP_022959115.1 (vestitone reductase-like [Cucurbita moschata])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 318/332 (95.78%), Postives = 327/332 (98.49%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLE GYSVTTTVRADPEMR+DYGFLT+LPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTSLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLD+PNSFAPAIAGCIGVFHLATPID SDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAP GRSYPISKTLTE+AVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPFGRSYPISKTLTERAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKA+YGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSH+IT+EEL NLFSAKYPEFQIPS ESLKDVKGY+FSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 333
           LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 332

BLAST of CmaCh17G000330 vs. NCBI nr
Match: KAG7013375.1 (hypothetical protein SDJN02_23539, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.6 bits (1690), Expect = 2.3e-184
Identity = 318/332 (95.78%), Postives = 326/332 (98.19%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLE GYSVTTTVRADPEMR+DYGFLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLD+PNSFAPAIAGCIGVFHLATPID SDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVD LDESCWSDIDYIN+IAP GRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKA+YGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSH+IT+EEL NLFSAKYPEFQIPS ESLKDVKGY+FSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 333
           LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETN 332

BLAST of CmaCh17G000330 vs. NCBI nr
Match: XP_023548535.1 (vestitone reductase-like [Cucurbita pepo subsp. pepo] >XP_023548536.1 vestitone reductase-like [Cucurbita pepo subsp. pepo] >XP_023548538.1 vestitone reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 648.3 bits (1671), Expect = 3.6e-182
Identity = 315/331 (95.17%), Postives = 324/331 (97.89%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLE GYSVTTTVRADPEMR+DYGFLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLD+PNSFAPAIAGCIGVFHLATPID SDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
            VRRVVYTSAAATMQFNHHTVD LDESCWSDIDYIN+IAP GRSYPISKTLTEQAVLEFS
Sbjct: 121 MVRRVVYTSAAATMQFNHHTVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKA+YGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSH+IT++EL NLFSAKYPEFQIPS ESLKDVKGYIFSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLDELVNLFSAKYPEFQIPSSESLKDVKGYIFSDVSSKKL 300

Query: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHET 332
           LDAGFQYKYGIEEMFDGAIQSCKEKGYL+ET
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLYET 331

BLAST of CmaCh17G000330 vs. NCBI nr
Match: KAG6574794.1 (hypothetical protein SDJN03_25433, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 606.7 bits (1563), Expect = 1.2e-169
Identity = 295/309 (95.47%), Postives = 303/309 (98.06%), Query Frame = 0

Query: 1   MENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKL 60
           MENGAKGKVCVTGGTGFIASWLIKSLLE GYSVTTTVRADPEMR+DYGFLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTNLPGASEKL 60

Query: 61  QIYHADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSK 120
           QIYHADLD+PNSFAPAIAGCIGVFHLATPID SDKEGVEVLTRRTIEGTLGILKVCLDSK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 121 TVRRVVYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFS 180
           TVRRVVYTSAAATMQFNHHTVD LDESCWSDIDYIN+IAP GRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 180

Query: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 240
           QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKA+YGLILKSNMVHVDDVAR+H
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 241 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 300
           IFLFEHPNANGRYVCSSH+IT+EEL NLFSAKYPEFQIPS ESLKDVKGY+FSDVSSKKL
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 301 LDAGFQYKY 310
           LDAGFQYKY
Sbjct: 301 LDAGFQYKY 309

BLAST of CmaCh17G000330 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 5.4e-83
Identity = 162/328 (49.39%), Postives = 219/328 (66.77%), Query Frame = 0

Query: 7   GKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPE-MRRDYGFLTNLPGASEKLQIYHA 66
           G VCVTGG+GF+ASWLI  LL+ GYSV  TVR + E  ++D  +LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 67  DLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRV 126
           DL++P SF PAI GC  VFH+A P+D +  E  E +T+RT++G +GILK CLD+KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 127 VYTSAAATMQF---NHHTVDFLDESCWSDIDYINSIAP--LGRSYPISKTLTEQAVLEFS 186
            YTS+A T+ +   N      +DES WSD++   +     +  SY +SK   E A LEF 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 187 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSH 246
            + GLEVVT++   VVGPFI   LP S+ ++ +++ GN  E  L    NMVH+DDVAR+ 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 247 IFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKKL 306
           IFL E P A GRY+CSS  + I+E+    S K+P+FQ+PS + L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 307 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
             AGF++KYG EE+F GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

BLAST of CmaCh17G000330 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 143/328 (43.60%), Postives = 206/328 (62.80%), Query Frame = 0

Query: 6   KGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHA 65
           K  VCVTG +GFI SWL+  LLE GY V  TVR DP   +    L +LP A   L ++ A
Sbjct: 5   KETVCVTGASGFIGSWLVMRLLERGYFVRATVR-DPGNLKKVQHLLDLPNAKTLLTLWKA 64

Query: 66  DLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRV 125
           DL +  S+  AI GC GVFH+ATP+D   K+    + + T+ G LGI+K C+ +KTVRR 
Sbjct: 65  DLSEEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRF 124

Query: 126 VYTSAAATMQFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQQYGL 185
           V+TS+A T+    H  +  DE+ WSD+++I S    G  Y +SKTL E+A  +F+++ GL
Sbjct: 125 VFTSSAGTVNVEEHQKNVYDENDWSDLEFIMSKKMTGWMYFVSKTLAEKAAWDFAEEKGL 184

Query: 186 EVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARSHIFLFE 245
           + ++++PT VVGPFI   +P S+    S ++ N+A Y +I +   VH+DD+  +HIFL+E
Sbjct: 185 DFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIRQGQYVHLDDLCNAHIFLYE 244

Query: 246 HPNANGRYVCSSHLITIEELANLFSAKYPEFQIPS-----PESLKDVKGYIFSDVSSKKL 305
              A GRY+CSSH  TI  ++     KYPE+ +PS      E+LK +      + SSKKL
Sbjct: 245 QAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDENLKSI------EFSSKKL 304

Query: 306 LDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
            D GF +KY +EEMF  +I++C++KG+L
Sbjct: 305 TDMGFNFKYSLEEMFIESIETCRQKGFL 325

BLAST of CmaCh17G000330 vs. TAIR 10
Match: AT4G35420.1 (dihydroflavonol 4-reductase-like1 )

HSP 1 Score: 244.2 bits (622), Expect = 1.5e-64
Identity = 137/329 (41.64%), Postives = 195/329 (59.27%), Query Frame = 0

Query: 5   AKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYH 64
           AKGKVCVTG +GF+ASWL+K LL  GY V  TVR DP   +    L  L GA E+L++  
Sbjct: 4   AKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVR-DPGNEKKLAHLWKLEGAKERLRLVK 63

Query: 65  ADLDDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRR 124
           ADL +  SF  AI GC GVFH A+P+        E + R  IEGTL +L+ C  + +++R
Sbjct: 64  ADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSLKR 123

Query: 125 VVYTSAAATMQFNHHTVDF-----LDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEF 184
           VV TS+++T++      DF     LDES W+ ++           Y +SKTL EQA  +F
Sbjct: 124 VVLTSSSSTVRIRD---DFDPKIPLDESIWTSVELCKRFQVW---YALSKTLAEQAAWKF 183

Query: 185 SQQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSNMVHVDDVARS 244
           S++ G+++VTVLP+++VGP + P L  +      L+ G   ++    +   VH+DDVAR+
Sbjct: 184 SEENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVART 243

Query: 245 HIFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPESLKDVKGYIFSDVSSKK 304
           HI +FEH  A GRY+CSS++I++EEL +  SA+YP   IP      +   Y   D  + K
Sbjct: 244 HIVVFEHEAAQGRYICSSNVISLEELVSFLSARYPSLPIPKRFEKLNRLHY---DFDTSK 303

Query: 305 LLDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           +   G ++K  +EEMFD  I S  E+GYL
Sbjct: 304 IQSLGLKFK-SLEEMFDDCIASLVEQGYL 321

BLAST of CmaCh17G000330 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 3.2e-59
Identity = 123/332 (37.05%), Postives = 198/332 (59.64%), Query Frame = 0

Query: 8   KVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQIYHADL 67
           K CV GGTG +AS LIK LL++GY V TTVR DPE  +    L  L    + L+I+ ADL
Sbjct: 12  KACVIGGTGNLASILIKHLLQSGYKVNTTVR-DPENEKKIAHLRKLQELGD-LKIFKADL 71

Query: 68  DDPNSFAPAIAGCIGVFHLATPIDMSDKEGVEVLTRRTIEGTLGILKVCLDSKTVRRVVY 127
            D +SF  + +GC  +FH+ATPI+   ++  + + +  I+G + +LK CL SK+V+RV+Y
Sbjct: 72  TDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVIY 131

Query: 128 TSAAATMQFNH--HTVDFLDESCWSDIDYINSIAPLGRSYPISKTLTEQAVLEFSQQYGL 187
           TS+AA +  N+   T   ++E  W+D++++    P    YPISK L E+   EF+++  +
Sbjct: 132 TSSAAAVSINNLSGTGIVMNEENWTDVEFLTEEKPFNWGYPISKVLAEKTAWEFAKENKI 191

Query: 188 EVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGN-------KAEYGLILKSNMVHVDDVAR 247
            +VTV+P  + G  +    P S+ ++ S ++G        K    L    + VHVDD+AR
Sbjct: 192 NLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFVHVDDLAR 251

Query: 248 SHIFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPS--PESLKDVKGYIFSDVS 307
           +H+FL E   A+GRY+C ++  ++ E+A+    +YP++ + S   E L   K      +S
Sbjct: 252 AHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPK----LTLS 311

Query: 308 SKKLLDAGFQYKYGIEEMFDGAIQSCKEKGYL 329
           S+KL++ GF+++YGI EM+D  I+  + KG +
Sbjct: 312 SQKLINEGFRFEYGINEMYDQMIEYFESKGLI 337

BLAST of CmaCh17G000330 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-51
Identity = 111/338 (32.84%), Postives = 191/338 (56.51%), Query Frame = 0

Query: 2   ENGAKGKVCVTGGTGFIASWLIKSLLENGYSVTTTVRADPEMRRDYGFLTNLPGASEKLQ 61
           E       CVTG +G+I SWL+KSLL+ GY+V  T+R   ++ +   F +     +E+L+
Sbjct: 6   EESKTATYCVTGASGYIGSWLVKSLLQRGYTVHATLR---DLAKSEYFQSKWK-ENERLR 65

Query: 62  IYHADLDDPNSFAPAIAGCIGVFHLATPIDM---SDKEGVEVLTRR-----TIEGTLGIL 121
           ++ ADL D  SF  A+ GC GVFH+A  ++    SD   +E   +       ++G   +L
Sbjct: 66  LFRADLRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVL 125

Query: 122 KVCLDSKTVRRVVYTSAAATM---QFNHHTVDFLDESCWSDIDYINSIAPLGRSYPISKT 181
             CL SK+V+RVV+TS+ +T+     N     F+DE+C + +D++      G  Y +SK 
Sbjct: 126 SSCLKSKSVKRVVFTSSISTLTAKDENERMRSFVDETCKAHVDHVLKTQASGWIYVLSKL 185

Query: 182 LTEQAVLEFSQQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKAEYGLILKSN- 241
           ++E+    ++++ G+++V+V+ T V GPF+ P +P S++V  S ++G+   + ++   N 
Sbjct: 186 VSEEEAFRYAKERGMDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILSAVNK 245

Query: 242 ------MVHVDDVARSHIFLFEHPNANGRYVCSSHLITIEELANLFSAKYPEFQIPSPES 301
                 +VH++D+ R+H+FL E P A G+Y+C    I + EL     +K    ++     
Sbjct: 246 RMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQKVNE 305

Query: 302 LKDVKGYIFSDVSSKKLLDAGFQYKYGIEEMFDGAIQS 322
            ++ +  +   +SSKKL + GF+YKYGIEE+ D  I +
Sbjct: 306 DEEERECMKPIISSKKLRELGFEYKYGIEEIVDQTIDA 339

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q403163.5e-11157.59Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1[more]
O221337.6e-8249.39Protein BRI1-5 ENHANCED 1 OS=Arabidopsis thaliana OX=3702 GN=BEN1 PE=2 SV=1[more]
I3PLR31.6e-7948.16Noscapine synthase SDR1 OS=Papaver somniferum OX=3469 GN=SDR1 PE=1 SV=1[more]
Q5XLY03.0e-7843.71Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1[more]
Q9XES55.1e-7844.00Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestic... [more]
Match NameE-valueIdentityDescription
A0A6J1KZ903.6e-191100.00vestitone reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498944 PE=3 SV=1[more]
A0A6J1H5D71.1e-18495.78vestitone reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111460207 PE=3 SV=1[more]
A0A5D3DLI48.0e-16787.20Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CBT58.0e-16787.20vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103499024 PE=3 SV=1[more]
A0A0A0K9S93.0e-16687.20Cinnamoyl-CoA reductase 16 OS=Cucumis sativus OX=3659 GN=CCR16 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023006100.17.3e-191100.00vestitone reductase-like [Cucurbita maxima][more]
XP_022959115.12.3e-18495.78vestitone reductase-like [Cucurbita moschata][more]
KAG7013375.12.3e-18495.78hypothetical protein SDJN02_23539, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023548535.13.6e-18295.17vestitone reductase-like [Cucurbita pepo subsp. pepo] >XP_023548536.1 vestitone ... [more]
KAG6574794.11.2e-16995.47hypothetical protein SDJN03_25433, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G45400.15.4e-8349.39NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G42800.12.0e-7743.60dihydroflavonol 4-reductase [more]
AT4G35420.11.5e-6441.64dihydroflavonol 4-reductase-like1 [more]
AT1G61720.13.2e-5937.05NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G27250.11.1e-5132.84NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 6..328
e-value: 1.3E-117
score: 394.9
NoneNo IPR availablePANTHERPTHR10366:SF563VESTITONE REDUCTASE-LIKEcoord: 7..320
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 7..320
NoneNo IPR availableCDDcd08958FR_SDR_ecoord: 9..305
e-value: 1.93655E-133
score: 379.611
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 9..250
e-value: 8.3E-22
score: 77.8
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 6..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh17G000330.1CmaCh17G000330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
molecular_function GO:0003824 catalytic activity