CmaCh16G012700 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G012700
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionganglioside-induced differentiation-associated protein 2-like
LocationCma_Chr16: 9679422 .. 9681281 (-)
RNA-Seq ExpressionCmaCh16G012700
SyntenyCmaCh16G012700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTTTGCCCCCACGTCCGCGCCATTCGCCTCTTCCGCCCAATGATCTGGAGTCCCTTTTAATCCTCTCCCGATCATCTTTATTTCTTCATTTATTTTCCAAATCTCATCCTCCAATTCCGTTCTCCACCAAAATCCCTTCACTCCCTCCTTATCCCTAACCATCCTCTCAACGCTACTCGCTTTTTCCTTCTGATTCTACTTGGAATTCTTCTCATATGGCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAAACCTCGACGTCTTCAAGATTAAAGGCAGAGACAAACAAGGCCGCCGGATTCTCCGCATTACCGGAAAGTTCTTTCCAGGTTCCCTCTCTCTCTCTCTCTTTCTCTCATTCTTTATCGGTGAAATTAATTTTTTGTCGTTTCTTTTGACCATTGAGTTTGAATGTGCTCAAGAAACATCTGTATGTTTTCCTTACCATGATCTGTTTCTGTTTTCTTGCTCTTTTCATAGATGAAATTAAATTTCTCTCGTTGTCATCCGCGCGAACTGTACATTTGAATGTGCTCAAGAAGAATATGAAATTTTCCTTAATTTGCTCTGATTCGATTTTCTTGCTCTGGTCTGCTTTTTTTTTTTTTTTAAATTGAATTTTCACAGTGAAGCATATATAGATTTGCCTTGCTATGCTCTGATTCGGTTTTCTTGCTCTGTTCTGTTTCTGTTTTCTTGTTCTTGACTGTGCTTAAGAAGTATCTGCATATTTGCACTGCTGTGCTCTGATTAAATTTCTAGCTGTTTTGAGCGATGAGATTGATGTTTTCTTTTTTTGAAAATCAGCACGGATTGTGAGTTTGGATGTGGTGAAGAAGCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTTACGATCTTGTACTTTCACACCGACGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCCCTCCGATCAATCTATGAAGCAATTCCGGCCACCGTCAAGGCTAATCTCGAGGCCGTGTACTTCGTTCACCCAGACCTCCAGGCCAGGCTCTTCCTCGCCACACTCGGCCGCATTTTCTTCACTAGCGAGTATATTTTCTAACCCTTCTTCTTCAATCAATTTCAGGTTTTCCCTATATTCTTATTCCCAATATTTTCCTTTTTGAATCCTAGAAAATAATGTAATTTTTATGAATTTTTCTGTATTTTTCTAAATTAAGAAAAACACTGATAATATACTATAAAAGCACTTTTTAATTTATTTCAAATATCTTAATTTATTAGATCCAATAACAAATATACTCTGAATTTTGAATTTAGTTTTATTGTGGTCCTCCATCTTTTTTTTTTTTTTTTCTAATATGTAATTGCATTTTTAAAATTTATAAATGTATTAGAAATAAAATTGAAAAGTTTAATTAAATATTTACCTTTTTTTTTTAATGCACTCAAAGACATTATTTTATACACCTTACCCAATAAAGGGATAATGATAAAAAATAAAATTAAAATATTAAAAAGGAAAAGTAGGAATAATTTTATGATGTTTTGTTGATAATATTACGTTATGGTTCATAATGAGAGGGAATGTTTGGATGTGGTAGGATGTATGGGAAGATAAAGTACGTGAGTAGAATTGATCTGCTGTGGGAGCACGTACGGAGGAACGAGATCGAGGTTCCAGAGTTCATATACGATCACGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCTGGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATTGATTCGCACGTGTACTCGATGAGGTGCATTTCCTAGATTCCTTTCTTCTTTGTTTTAAAGAAAGGGTGGCCTTTGTTTAA

mRNA sequence

ATCTTTGCCCCCACGTCCGCGCCATTCGCCTCTTCCGCCCAATGATCTGGAGTCCCTTTTAATCCTCTCCCGATCATCTTTATTTCTTCATTTATTTTCCAAATCTCATCCTCCAATTCCGTTCTCCACCAAAATCCCTTCACTCCCTCCTTATCCCTAACCATCCTCTCAACGCTACTCGCTTTTTCCTTCTGATTCTACTTGGAATTCTTCTCATATGGCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAAACCTCGACGTCTTCAAGATTAAAGGCAGAGACAAACAAGGCCGCCGGATTCTCCGCATTACCGGAAAGTTCTTTCCAGCACGGATTGTGAGTTTGGATGTGGTGAAGAAGCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTTACGATCTTGTACTTTCACACCGACGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCCCTCCGATCAATCTATGAAGCAATTCCGGCCACCGTCAAGGCTAATCTCGAGGCCGTGTACTTCGTTCACCCAGACCTCCAGGCCAGGCTCTTCCTCGCCACACTCGGCCGCATTTTCTTCACTAGCGAGATGTATGGGAAGATAAAGTACGTGAGTAGAATTGATCTGCTGTGGGAGCACGTACGGAGGAACGAGATCGAGGTTCCAGAGTTCATATACGATCACGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCTGGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATTGATTCGCACGTGTACTCGATGAGGGTGGCCTTTGTTTAA

Coding sequence (CDS)

ATGGCCGCTCAGGTTCAGCCTCCTCCGCCGGAGCAAAACCAACTCGAAAACCTCGACGTCTTCAAGATTAAAGGCAGAGACAAACAAGGCCGCCGGATTCTCCGCATTACCGGAAAGTTCTTTCCAGCACGGATTGTGAGTTTGGATGTGGTGAAGAAGCATCTGGAGGAGAAGATCTTCCCTAGGCTTAAGAACAAGCGTTTTACGATCTTGTACTTTCACACCGACGTTCAAAGGAGCCAAAATTTCCCCGGAATCGCCGCCCTCCGATCAATCTATGAAGCAATTCCGGCCACCGTCAAGGCTAATCTCGAGGCCGTGTACTTCGTTCACCCAGACCTCCAGGCCAGGCTCTTCCTCGCCACACTCGGCCGCATTTTCTTCACTAGCGAGATGTATGGGAAGATAAAGTACGTGAGTAGAATTGATCTGCTGTGGGAGCACGTACGGAGGAACGAGATCGAGGTTCCAGAGTTCATATACGATCACGATGAAGATCTTGAGTACCGTCCGATGATGGACTATGGTCTGGAGAGTGACCATCCTAGGGTTTACGGTGCACCCTCAATTGATTCGCACGTGTACTCGATGAGGGTGGCCTTTGTTTAA

Protein sequence

MAAQVQPPPPEQNQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIFPRLKNKRFTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIFFTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESDHPRVYGAPSIDSHVYSMRVAFV
Homology
BLAST of CmaCh16G012700 vs. ExPASy Swiss-Prot
Match: Q7JUR6 (Protein GDAP2 homolog OS=Drosophila melanogaster OX=7227 GN=Gdap2 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.0e-12
Identity = 39/140 (27.86%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 25  GRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIFPRLKNKRFTILYFHTDVQRSQNFP 84
           G D+ GR ++   GK+FPA+ + L+    +L + + P +K   + I YFHT +  + N+P
Sbjct: 388 GVDRLGRPVIVFCGKWFPAQNIDLEKALLYLIKLLDPIVKGD-YVISYFHT-LTSTNNYP 447

Query: 85  GIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIFFTSEMYGKIKYVSRIDL 144
            +  LR +Y  +P   K NL+A Y VHP    ++ +      F    +  K+  +  ++ 
Sbjct: 448 SLHWLREVYSVLPYKYKKNLKAFYIVHPTFWTKM-MTWWFTTFMAPAIKAKVHSLPGVEH 507

Query: 145 LWEHVRRNEIEVPEFIYDHD 165
           L+  + ++++E+P +I ++D
Sbjct: 508 LYSAITKDQLEIPAYITEYD 524

BLAST of CmaCh16G012700 vs. ExPASy Swiss-Prot
Match: Q292F9 (Protein GDAP2 homolog OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA15091 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.0e-12
Identity = 39/140 (27.86%), Postives = 76/140 (54.29%), Query Frame = 0

Query: 25  GRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIFPRLKNKRFTILYFHTDVQRSQNFP 84
           G D+ GR ++   GK+FPA+ + L+    +L + + P +K   + I YFHT +  + N+P
Sbjct: 390 GVDRLGRPVIVFCGKWFPAQNIDLEKALLYLIKLLDPIVKGD-YVISYFHT-LTSTNNYP 449

Query: 85  GIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIFFTSEMYGKIKYVSRIDL 144
            +  LR +Y  +P   K NL+A Y VHP    ++ +      F    +  K+  +  ++ 
Sbjct: 450 SLHWLREVYSVLPYKYKKNLKAFYIVHPTFWTKM-MTWWFTTFMAPAIKAKVHSLPGVEH 509

Query: 145 LWEHVRRNEIEVPEFIYDHD 165
           L+  + ++++E+P +I ++D
Sbjct: 510 LYSAITKDQLEIPAYITEYD 526

BLAST of CmaCh16G012700 vs. ExPASy Swiss-Prot
Match: A7T167 (Protein GDAP2 homolog OS=Nematostella vectensis OX=45351 GN=gdap2 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.2e-09
Identity = 33/140 (23.57%), Postives = 72/140 (51.43%), Query Frame = 0

Query: 25  GRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIFPRLKNKRFTILYFHTDVQRSQNFP 84
           G D  GR ++    + F A+   L     +    +  R+ N+ + ++YFHT     +N P
Sbjct: 346 GVDFLGRPVVVFVARHFTAQNTDLGKAVAYF-ISVLDRIVNRDYVVVYFHTH-STEENQP 405

Query: 85  GIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIFFTSEMYGKIKYVSRIDL 144
            ++ L+ +Y  +    + NL+A Y VHP + AR+ +      F  S +  K+ ++S +  
Sbjct: 406 PMSFLKELYHIVDNKYRRNLKAFYIVHPTVWARI-VTWFFTTFTASSVKEKVHFLSGVQY 465

Query: 145 LWEHVRRNEIEVPEFIYDHD 165
           L++ +  +++++P ++ ++D
Sbjct: 466 LYDWINPDQLDIPAYVLEYD 482

BLAST of CmaCh16G012700 vs. ExPASy Swiss-Prot
Match: Q0IHU9 (Protein prune homolog 2 OS=Xenopus tropicalis OX=8364 GN=Prune2 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 9.2e-06
Identity = 32/100 (32.00%), Postives = 52/100 (52.00%), Query Frame = 0

Query: 68  FTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIF 127
           + ++Y +    R +  PG+  ++  Y+ I   ++ NL++   VHP    R  LA L R F
Sbjct: 187 YMVVYLNGATPR-RKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILA-LTRPF 246

Query: 128 FTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDL 168
            +S+   KIKYVS +  L E +    + +PE I   DE+L
Sbjct: 247 ISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKLDEEL 284

BLAST of CmaCh16G012700 vs. TAIR 10
Match: AT4G35750.1 (SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein )

HSP 1 Score: 275.0 bits (702), Expect = 4.8e-74
Identity = 131/200 (65.50%), Postives = 164/200 (82.00%), Query Frame = 0

Query: 1   MAAQVQPPPPEQNQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIF 60
           M++Q+     EQ  +E L++FKI GRDK+GR+ILRI GKFFPAR +SLDV+KK+LEEKIF
Sbjct: 1   MSSQISEIEQEQ-LIEKLEIFKIHGRDKRGRKILRIIGKFFPARFLSLDVLKKYLEEKIF 60

Query: 61  PRLKNKRFTILYFHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFL 120
           PRL  K F +LY HT VQRS+NFPGI+ALR+IY+AIP  V+ NL+ VYF+HP LQ+RLFL
Sbjct: 61  PRLGRKPFAVLYVHTGVQRSENFPGISALRAIYDAIPVNVRDNLQEVYFLHPGLQSRLFL 120

Query: 121 ATLGRIFFTSEMYGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 180
           AT GR  F+  +YGK++Y+SR+D LWEHVRRNEIE+PEF+YDHD+DLEYRPMMDYG ESD
Sbjct: 121 ATCGRFLFSGGLYGKLRYISRVDYLWEHVRRNEIEMPEFVYDHDDDLEYRPMMDYGQESD 180

Query: 181 HPRVYGAPSIDSHV--YSMR 199
           H RV+   ++DS V  +SMR
Sbjct: 181 HARVFAGAAVDSSVSSFSMR 199

BLAST of CmaCh16G012700 vs. TAIR 10
Match: AT3G10210.1 (SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein )

HSP 1 Score: 174.9 bits (442), Expect = 6.7e-44
Identity = 77/168 (45.83%), Postives = 117/168 (69.64%), Query Frame = 0

Query: 13  NQLENLDVFKIKGRDKQGRRILRITGKFFPARIVSLDVVKKHLEEKIFPRLKNKRFTILY 72
           + L+ L  F ++G D+ G RI RI GK+FPAR+VS + +KK++ +KI  +       ++Y
Sbjct: 47  SDLDLLQFFTLQGLDRSGNRIFRIVGKYFPARVVSAERLKKYISQKISNQCPEGPLCLVY 106

Query: 73  FHTDVQRSQNFPGIAALRSIYEAIPATVKANLEAVYFVHPDLQARLFLATLGRIFFTSEM 132
            H+ VQ+  N PGI  LR IYE +P+ +K  L+ VYF+HP L++RL +ATLGR+  +  +
Sbjct: 107 MHSTVQKDDNSPGITILRWIYEDLPSDIKDRLQLVYFIHPGLRSRLVIATLGRLLLSGGL 166

Query: 133 YGKIKYVSRIDLLWEHVRRNEIEVPEFIYDHDEDLEYRPMMDYGLESD 181
           Y KIKYVSR+  LWE +++ E+E+P+F+ +HD  LE+RP+ DYG+E D
Sbjct: 167 YWKIKYVSRLQYLWEDIKKGEVEIPDFVKNHDNVLEHRPLTDYGIEPD 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7JUR63.0e-1227.86Protein GDAP2 homolog OS=Drosophila melanogaster OX=7227 GN=Gdap2 PE=2 SV=1[more]
Q292F93.0e-1227.86Protein GDAP2 homolog OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=GA15... [more]
A7T1675.2e-0923.57Protein GDAP2 homolog OS=Nematostella vectensis OX=45351 GN=gdap2 PE=3 SV=1[more]
Q0IHU99.2e-0632.00Protein prune homolog 2 OS=Xenopus tropicalis OX=8364 GN=Prune2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G35750.14.8e-7465.50SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein... [more]
AT3G10210.16.7e-4445.83SEC14 cytosolic factor family protein / phosphoglyceride transfer family protein... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001251CRAL-TRIO lipid binding domainSMARTSM00516sec14_4coord: 14..163
e-value: 2.3E-11
score: 53.8
IPR001251CRAL-TRIO lipid binding domainPFAMPF13716CRAL_TRIO_2coord: 30..166
e-value: 1.3E-27
score: 96.7
IPR001251CRAL-TRIO lipid binding domainCDDcd00170SEC14coord: 25..157
e-value: 3.44656E-10
score: 54.3134
IPR036865CRAL-TRIO lipid binding domain superfamilyGENE3D3.40.525.10coord: 13..168
e-value: 1.0E-25
score: 92.1
IPR036865CRAL-TRIO lipid binding domain superfamilySUPERFAMILY52087CRAL/TRIO domaincoord: 20..154
NoneNo IPR availablePANTHERPTHR11106:SF103OS04G0561200 PROTEINcoord: 10..199
NoneNo IPR availablePANTHERPTHR11106GANGLIOSIDE INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 2-RELATEDcoord: 10..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G012700.1CmaCh16G012700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane