CmaCh16G012660 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G012660 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 7.6e-130
Identity = 257/549 (46.81%), Postives = 344/549 (62.66%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           M+      AN VPLSP+ F +R++  Y    S++YGS  +TW++T+ RCL LASAL  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
            ISPGDVVA  + N+PE+YELHFAVPMAG I+  LN + D+  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEF-TPRTNSEL 221
             L T   +L       I+ P LVL+        S +  YN +L    D+F   R  +E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208

Query: 222 DAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWC 281
           D ISIN TSG+T   K VVYSHR AYLNS+AT+   G+    +  V+LW+V MF CNGWC
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM---GTRSVYLWSVPMFHCNGWC 268

Query: 282 FIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLR 341
           F W  AA G  NIC+R V+P AIF NI LHKVT      T+L MI  S   N   P    
Sbjct: 269 FPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-LPH 328

Query: 342 RVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE 401
           +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA     KP +D    EE    +
Sbjct: 329 KVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALK 388

Query: 402 -----DLIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDW 461
                + +A  E+DV+DP SMESV  DG T+GEVM RGN++MSGY+K+LKAT EAF G W
Sbjct: 389 ARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGW 448

Query: 462 YRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI----- 521
           +RTGD+GVKH+ G I++KDR KD+V+  +G   VSTV+VE VL SH  V EAAV+     
Sbjct: 449 FRTGDLGVKHEDGYIQLKDRKKDVVI--SGGENVSTVEVETVLYSHEAVLEAAVVARPDK 508

Query: 522 --GERLCGLVKLKNG--SRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIA 572
             GE  C  V LK G  +  SA++I++FCR  LP +M P+ VVF +LP  S GK+QK+I 
Sbjct: 509 LWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYIL 568

BLAST of CmaCh16G012660 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 1.1e-128
Identity = 261/560 (46.61%), Postives = 352/560 (62.86%), Query Frame = 0

Query: 48  SPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGD 107
           SPANF PLSP+ F +R+A VY  R S+++GS   TW +TY RCL LASAL  +  IS GD
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 108 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTL 167
           VVAA+APNVP ++ELHFAVPMAG I+ PLNT+LD  TLS+LL     K++F+D   L   
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 168 -----LQSLSQTYGNSIQFPALVLIPAHPDTPPSE-------------FLDYNRVLEMRF 227
                L + S     S++   LVLI    D   S+               +Y  +L+   
Sbjct: 174 HGALDLLAKSDKTRKSLK---LVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGD 233

Query: 228 DEF-TPRTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFL 287
            EF   +   E D ISIN TSG+T   KGVVYSHR AYLNSLAT+F   + + +  PV+L
Sbjct: 234 SEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF---LHQMSVYPVYL 293

Query: 288 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES 347
           WTV MF CNGWC +W +AA GG NICLR V+P  IF NI +HKVT + G  T+L MI  +
Sbjct: 294 WTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIV-N 353

Query: 348 SSFNNCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF 407
            +    KP    RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WKP +
Sbjct: 354 YTVTEHKP-LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEW 413

Query: 408 DEETVQFEDLIASLE---------IDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKN 467
           D  +++    + + +         +DVKDP +ME+V  DG T+GEVM RGN++MSGY+K+
Sbjct: 414 DSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKD 473

Query: 468 LKATGEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPG 527
           ++AT +AF GDW+ +GD+ VK+  G IE+KDR KD+++  +G   +S+V+VE VL SH  
Sbjct: 474 IEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLCSHQA 533

Query: 528 VAEAAVI-------GERLCGLVKLKNG-SRASAEEIVEFCRAHLPEFMIPERVVFGDLPM 572
           V EAAV+       G+  CG VKLK G      EEI+ FCR HLP +M P+ +VFGD+P 
Sbjct: 534 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 593

BLAST of CmaCh16G012660 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.9e-128
Identity = 257/553 (46.47%), Postives = 343/553 (62.03%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           M+    SPAN+VPL+P++F  R+A VY  R S++YGS  +TW +T  RC+ +ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
            IS GDVV+ +APNVP + ELHF VPMAGA++  LN + D+  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTP-------PSEFLDYNRVLEMRFDEF-T 221
            FL     +         + P LVLIP  P T          E ++Y  V+ M   +F  
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 222 PRTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDM 281
            R   E DAIS+N TSG+T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  M
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPM 242

Query: 282 FRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES-SSFN 341
           F CNGWC +W + A+GG NICLRNVT  AIF NI  HKVT + G  T+L MI  +  S  
Sbjct: 243 FHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQ 302

Query: 342 NCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD--- 401
              PG   +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D   
Sbjct: 303 KPLPG---KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLP 362

Query: 402 -EETVQFE-----DLIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKAT 461
            EE  + +     + +   EI VKDP +M ++  DG T+GEV+ RGN++M+GY KN +AT
Sbjct: 363 REEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEAT 422

Query: 462 GEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEA 521
            EAF G W+ +GD+GVKH  G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EA
Sbjct: 423 KEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEA 482

Query: 522 AVI-------GERLCGLVKLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGK 570
           AV+       GE  C  VKLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GK
Sbjct: 483 AVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGK 542

BLAST of CmaCh16G012660 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.6e-127
Identity = 252/549 (45.90%), Postives = 343/549 (62.48%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           M+      AN VPLSP+ F +R++  Y    S++YGS  +TW++T+ RCL LASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
            ISPGDVVA  + N+PE+YELHFAVPMAG I+  LN + D+  +S LL     K+IF++ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEF-TPRTNSEL 221
             L T   +L       I+ P LVL+        S +  YN +L    D+F   R  +E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208

Query: 222 DAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWC 281
           D ISIN TSG+T   K VVYSHR AYLNS+AT+   G+    ++ V+LW+V MF CNGWC
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM---GTTSVYLWSVPMFHCNGWC 268

Query: 282 FIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLR 341
           F W  AA G  NIC+R V+P AIF NI LHKVT      T+L MI  S   N   P    
Sbjct: 269 FPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-LPH 328

Query: 342 RVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE 401
           +V+++  G+ P  +++ ++ E+GF +++ YG+TE  GPA     KP +D    EE    +
Sbjct: 329 KVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALK 388

Query: 402 -----DLIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDW 461
                + +A  E+DV+DP +MESV  DG T+GEVM RGN++MSGY+K+LKAT EAF G W
Sbjct: 389 ARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGW 448

Query: 462 YRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI----- 521
           +R+GD+GVKH+ G I++KDR KD+V+  +G   +STV+VE VL SH  V EAAV+     
Sbjct: 449 FRSGDLGVKHEDGYIQLKDRKKDVVI--SGGENISTVEVETVLYSHEAVLEAAVVARPDK 508

Query: 522 --GERLCGLVKLKNG--SRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIA 572
             GE  C  V LK G  +  SA++I++FCR  LP +M P+ VVF +LP  S GK+QK+I 
Sbjct: 509 LWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYIL 568

BLAST of CmaCh16G012660 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 7.9e-111
Identity = 232/561 (41.35%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           MD      AN VPL+P+ F KRA+  Y  R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
           +I+  DVV+ +APNVP +YE+HF+VPM GA+++P+NT+LDA T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 162 DFLPTLLQ--SLSQTYGNSIQFPALVLIPAHPDT--PPSEFLDYNRVLEMRFDEFTP--- 221
           +F P + +   L  TY  S   P ++LI     T  P S+ LDY  ++  R  E TP   
Sbjct: 121 EFAPLIQEVLRLIPTY-QSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSS 180

Query: 222 ----RTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFL 281
               R ++E D IS+N TSG+T   KGVV SH+ AYL++L++I  +  GI      PV+L
Sbjct: 181 ASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI-----FPVYL 240

Query: 282 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES 341
           WT+ MF CNGW   W++AA GG N+C+R+VT   I+ NIELH VT +    T+ + + E 
Sbjct: 241 WTLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEG 300

Query: 342 SSFNNCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWK--- 401
           S  +         V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+   
Sbjct: 301 SRTDQSPKS--SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEW 360

Query: 402 ---PNFDEETVQFEDLIASL---EIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKN 461
              P   +  +Q    + +L   ++DVK+  ++ESV  DG+T+GE++++G+SLM GY KN
Sbjct: 361 NKLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKN 420

Query: 462 LKATGEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPG 521
            KAT EAF   W  TGD+GV H  G +E+KDR+KDI++  +G   +S+++VE VL  +  
Sbjct: 421 PKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 522 VAEAAVI-------GERLCGLVKLKNGSR---ASAEEIVEFCRAHLPEFMIPERVV-FGD 570
           V EAAV+       GE  C  V LK G      S  +++++CR ++P FM P++VV F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of CmaCh16G012660 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 461.8 bits (1187), Expect = 7.8e-130
Identity = 261/560 (46.61%), Postives = 352/560 (62.86%), Query Frame = 0

Query: 48  SPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHFHISPGD 107
           SPANF PLSP+ F +R+A VY  R S+++GS   TW +TY RCL LASAL  +  IS GD
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 108 VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTL 167
           VVAA+APNVP ++ELHFAVPMAG I+ PLNT+LD  TLS+LL     K++F+D   L   
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 168 -----LQSLSQTYGNSIQFPALVLIPAHPDTPPSE-------------FLDYNRVLEMRF 227
                L + S     S++   LVLI    D   S+               +Y  +L+   
Sbjct: 174 HGALDLLAKSDKTRKSLK---LVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGD 233

Query: 228 DEF-TPRTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFL 287
            EF   +   E D ISIN TSG+T   KGVVYSHR AYLNSLAT+F   + + +  PV+L
Sbjct: 234 SEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF---LHQMSVYPVYL 293

Query: 288 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES 347
           WTV MF CNGWC +W +AA GG NICLR V+P  IF NI +HKVT + G  T+L MI  +
Sbjct: 294 WTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIV-N 353

Query: 348 SSFNNCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF 407
            +    KP    RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WKP +
Sbjct: 354 YTVTEHKP-LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEW 413

Query: 408 DEETVQFEDLIASLE---------IDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKN 467
           D  +++    + + +         +DVKDP +ME+V  DG T+GEVM RGN++MSGY+K+
Sbjct: 414 DSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKD 473

Query: 468 LKATGEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPG 527
           ++AT +AF GDW+ +GD+ VK+  G IE+KDR KD+++  +G   +S+V+VE VL SH  
Sbjct: 474 IEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLCSHQA 533

Query: 528 VAEAAVI-------GERLCGLVKLKNG-SRASAEEIVEFCRAHLPEFMIPERVVFGDLPM 572
           V EAAV+       G+  CG VKLK G      EEI+ FCR HLP +M P+ +VFGD+P 
Sbjct: 534 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 593

BLAST of CmaCh16G012660 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.3e-129
Identity = 257/553 (46.47%), Postives = 343/553 (62.03%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           M+    SPAN+VPL+P++F  R+A VY  R S++YGS  +TW +T  RC+ +ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
            IS GDVV+ +APNVP + ELHF VPMAGA++  LN + D+  +++LL+    KVIF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTP-------PSEFLDYNRVLEMRFDEF-T 221
            FL     +         + P LVLIP  P T          E ++Y  V+ M   +F  
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEV 182

Query: 222 PRTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDM 281
            R   E DAIS+N TSG+T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  M
Sbjct: 183 IRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPM 242

Query: 282 FRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES-SSFN 341
           F CNGWC +W + A+GG NICLRNVT  AIF NI  HKVT + G  T+L MI  +  S  
Sbjct: 243 FHCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQ 302

Query: 342 NCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD--- 401
              PG   +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D   
Sbjct: 303 KPLPG---KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLP 362

Query: 402 -EETVQFE-----DLIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKAT 461
            EE  + +     + +   EI VKDP +M ++  DG T+GEV+ RGN++M+GY KN +AT
Sbjct: 363 REEQAKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEAT 422

Query: 462 GEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEA 521
            EAF G W+ +GD+GVKH  G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EA
Sbjct: 423 KEAFKGGWFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEA 482

Query: 522 AVI-------GERLCGLVKLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGK 570
           AV+       GE  C  VKLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GK
Sbjct: 483 AVVARPDEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGK 542

BLAST of CmaCh16G012660 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 402.5 bits (1033), Expect = 5.6e-112
Identity = 232/561 (41.35%), Postives = 344/561 (61.32%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           MD      AN VPL+P+ F KRA+  Y  R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
           +I+  DVV+ +APNVP +YE+HF+VPM GA+++P+NT+LDA T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 162 DFLPTLLQ--SLSQTYGNSIQFPALVLIPAHPDT--PPSEFLDYNRVLEMRFDEFTP--- 221
           +F P + +   L  TY  S   P ++LI     T  P S+ LDY  ++  R  E TP   
Sbjct: 121 EFAPLIQEVLRLIPTY-QSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSS 180

Query: 222 ----RTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFL 281
               R ++E D IS+N TSG+T   KGVV SH+ AYL++L++I  +  GI      PV+L
Sbjct: 181 ASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI-----FPVYL 240

Query: 282 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES 341
           WT+ MF CNGW   W++AA GG N+C+R+VT   I+ NIELH VT +    T+ + + E 
Sbjct: 241 WTLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEG 300

Query: 342 SSFNNCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWK--- 401
           S  +         V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+   
Sbjct: 301 SRTDQSPKS--SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEW 360

Query: 402 ---PNFDEETVQFEDLIASL---EIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKN 461
              P   +  +Q    + +L   ++DVK+  ++ESV  DG+T+GE++++G+SLM GY KN
Sbjct: 361 NKLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKN 420

Query: 462 LKATGEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPG 521
            KAT EAF   W  TGD+GV H  G +E+KDR+KDI++  +G   +S+++VE VL  +  
Sbjct: 421 PKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 522 VAEAAVI-------GERLCGLVKLKNGSR---ASAEEIVEFCRAHLPEFMIPERVV-FGD 570
           V EAAV+       GE  C  V LK G      S  +++++CR ++P FM P++VV F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of CmaCh16G012660 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 386.0 bits (990), Expect = 5.4e-107
Identity = 227/575 (39.48%), Postives = 330/575 (57.39%), Query Frame = 0

Query: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101
           MD      AN VPL+P+ F KRA+  Y  R S+IYG   FTW +TY RC  LA++L+   
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161
           +IS  DVV+ MAPN P LYE+HFAVPMAGA+++P+NT+LDA +++ +L+   PK++FLD 
Sbjct: 61  NISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDR 120

Query: 162 DFLPTLLQSLS--QTYGNSIQFPALVLIP-AHPDTPPSEFLDYNRVLEMRFDEFTP---- 221
            F     +SL    +  +++  P + +     P     E LDY  +++    E TP    
Sbjct: 121 SFEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQR--GEPTPSMVA 180

Query: 222 ---RTNSELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFLW 281
              R   E D IS+N TSG+T   KGVV SHR AYL +L+ I  +  G C     PV+LW
Sbjct: 181 RMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTC-----PVYLW 240

Query: 282 TVDMFRCNGWCFIWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESS 341
           T+ MF CNGW F W  AA GG ++C+R+VT   I+ NIE+H VT +C   T+  ++ + +
Sbjct: 241 TLPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGN 300

Query: 342 SFN-NCKPGFLRRVDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF 401
           S + + + G    V ++  G+ P   ++ KV  LGF + + YG TEA GP +   W+  +
Sbjct: 301 SLDLSPRSG---PVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEW 360

Query: 402 DE--ETVQFE-------DLIASLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKN 461
           +   E  Q E        ++   ++DVK+  + +S   DG+T+GE++++G+S+M GY KN
Sbjct: 361 NRLPENQQMELKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKN 420

Query: 462 LKATGEAFSGDWYRTGDVGVKHKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPG 521
            KAT EAF   W  TGDVGV H  G +E+KDR+KDI++  +G   +S+V+VE VL  +P 
Sbjct: 421 PKATFEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIII--SGGENISSVEVENVLYKYPK 480

Query: 522 VAEAAVI-------GERLCGLVKLKNGSRASAEE-----------IVEFCRAHLPEFMIP 576
           V E AV+       GE  C  V L+       E+           ++E+CR +LP FM P
Sbjct: 481 VLETAVVAMPHPTWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCP 540

BLAST of CmaCh16G012660 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 384.8 bits (987), Expect = 1.2e-106
Identity = 218/476 (45.80%), Postives = 290/476 (60.92%), Query Frame = 0

Query: 119 LYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDSDFLPTLLQSLSQTYGNS 178
           + ELHF VPMAGA++  LN + D+  +++LL+    KVIF D  FL     +        
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 179 IQFPALVLIPAHPDTP-------PSEFLDYNRVLEMRFDEF-TPRTNSELDAISINCTSG 238
            + P LVLIP  P T          E ++Y  V+ M   +F   R   E DAIS+N TSG
Sbjct: 61  DKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSG 120

Query: 239 STGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAALGG 298
           +T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  MF CNGWC +W + A+GG
Sbjct: 121 TTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGG 180

Query: 299 CNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYES-SSFNNCKPGFLRRVDLIVAGA 358
            NICLRNVT  AIF NI  HKVT + G  T+L MI  +  S     PG   +V  I   A
Sbjct: 181 TNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG---KVSFITGAA 240

Query: 359 LPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE-----DLIA 418
            P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D    EE  + +     + + 
Sbjct: 241 PPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLG 300

Query: 419 SLEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVK 478
             EI VKDP +M ++  DG T+GEV+ RGN++M+GY KN +AT EAF G W+ +GD+GVK
Sbjct: 301 LEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVK 360

Query: 479 HKSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGL 538
           H  G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EAAV+       GE  C  
Sbjct: 361 HPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAF 420

Query: 539 VKLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKAL 570
           VKLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GKVQKF+ R KAKAL
Sbjct: 421 VKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467