CmaCh16G012380 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G012380
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Translocase of chloroplast
Location	Cma_Chr16: 9365290 .. 9367550 (-)
RNA-Seq Expression	CmaCh16G012380
Synteny	CmaCh16G012380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGCGCACACAGCTTCATGGCATCCCAGGTTATCCGGGAGTGGATTGGGATTAACAACTTTGCAATGGCCACACAAGTCAAATTACTTGAGTTGCTGGGAAGACTGAAGCTGGAGGTCATATATTCATTTTTACCCATATGTCTTGGTCACGAATCCGTATCTACGTGATTTATTTTTCCATTTAAGCCTTTGCACTGCATTTTTCACAATCTCTAATGATTCATTAACTTTTGATATCTTTCTACAGAATGTCAGCACCTTGACCATACTTGTAATGGGAAAGGGTGGCGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGTTGTTTCTGTTAGTCCTTTTCAGGTACTTTAGTTATTCACTTCACTTTAATTTTGGTTGATTGAAGTGCACAGAAGGTTCATGATTTGATTTAAGAAATGAAATGTTTATAGTCAGAAGTGACAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACATTAAACATCATTGACACACCTGGTATCATAGAAGGAGGTTATATCAATGATCAGACACTTGAGATCATAAAACGGTAATTTCTAATATTATTTTTTACTTCAGATTGATTTTTTTCGAGAATCTGGGTCTTGTCTTTGTATTTCCTTATCTTTCTTTTTTGAGCAGCTTTCTTTTGAATAAGACCATAGATATTCTTCTCTATGTGGACCGCTTGGATGTATATCGAGTAGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAAACAGCTTTGGTAAAGCAATATGGAACAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCTGATGGAGTACCATATGAAGAATTTTTCTCCAGACGATCAGAAGCTCTACTGAAAACCGTCCGATTTGGCGCCTCTTTCAACAAAAAAGATATTCAGGTGGCTTTTGCTATTTTTGTTCGCCTTTGTGAGCAAATCTTGAGTTCCATTTTGTTTTCTGTACATTCTTTTATTGTATGCCTATATGTCTATGTGAACATATTTTTAGTCGATAATACTCATGTCATCACATTTGATCTTTCCTGCCCCTGTTTTCTTTCATTTTTCTCCATAAAAATTTGGTTTATCATTTAAAAAACTGAGATTTCAAGGAAAATCCTTGAATATCTTCAGTAAATTTTTTTTGTTACTGAAAGATTTCAACTGAAGATATTCAAGGATTTCCTGCCCATGTCTTTGATCTTTCTTTCAGTAAAATCTATCAGAATTCCGGCATGAGATGAAATCCATGTCTTCCTTTTGTTCCACAGAAGATTAGATTACTTAATTTATTTGTCGCTCATTGAATGATAAAAGTATTATCTAAATTCTATACCTTCTAGGCGTTGGATATTCCAGTTGTTTTGGTTGAGAACAGTGGACGGTGCAATAAGAACGAGAAAGATGAAAAGGTTCTACTTCTCTCTCTCTCTGTCTTAAAAATGTTCATTAGAATCTTTTCAACTTATTGGTTGGACTAATCCTTTTCTCAACAGGTTCTTCCAAATGGGATTGCTTGGATTCCTCACTTGGTTGAGACAATCACCGCAGTTGTGTTGAAAGAAGGAACCAAGTCTATTTTTGTTGATAAAAATTTGATCGCAGGCCCAAACCCCAATCAAAGGGGAAAGTTGCTGATTCCTTTTATTGTTGTACTCCAAGTATGCATCTGAAACTTAAAACGATTCTTTAATTTTCCTCTTCTCTTACGATTTAGGGACTGACCCCATATCTTTTTGCTTCTTATGTTGGCTTGTTATTCACAGTATCTCTTCGCTGTTAGACCTATTGAGCGAGCAATAAGAAGTGATATATTAAATGAAAGTAGACCATCGTGGGAGACGCATGAGTACTAGCTTATCCAGCCGCAGGTATTGATTTGGCCTCATAAATATCCCGAGCGATCAATATGGTTGTTTGGTGAGTTAATCTGAGTCTTTTTGTCAATGTTGAGATTGGACTGTCTTGTTTTTTTTTAAAGTTTGAATTTTGAGACAACTGAGTTTCTGTAAGCAAATTCATATACGCTACAAGGTTTAGCTACTCGTATTTAGTTAAACAAGAAATTCTTCAATTTTTTCTAGAAGCGGATGATTACTCATCTGCTTCTCACGTTTCGTGAATAGTTAGGACATGATTCTTTTTGTTCCCCAATCCCATCACTCTTCCCAATTGACTATCAAACTTTCAAACTTTGCATGCAAATGTTATAATGTTCATGATCGAGAT

mRNA sequence

ATGACAGGCGCACACAGCTTCATGGCATCCCAGGTTATCCGGGAGTGGATTGGGATTAACAACTTTGCAATGGCCACACAAGTCAAATTACTTGAGTTGCTGGGAAGACTGAAGCTGGAGAATGTCAGCACCTTGACCATACTTGTAATGGGAAAGGGTGGCGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGTTGTTTCTGTTAGTCCTTTTCAGTCAGAAGTGACAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACATTAAACATCATTGACACACCTGGTATCATAGAAGGAGGTTATATCAATGATCAGACACTTGAGATCATAAAACGCTTTCTTTTGAATAAGACCATAGATATTCTTCTCTATGTGGACCGCTTGGATGTATATCGAGTAGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAAACAGCTTTGGTAAAGCAATATGGAACAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCTGATGGAGTACCATATGAAGAATTTTTCTCCAGACGATCAGAAGCTCTACTGAAAACCGTCCGATTTGGCGCCTCTTTCAACAAAAAAGATATTCAGGCGTTGGATATTCCAGTTGTTTTGGTTGAGAACAGTGGACGGTGCAATAAGAACGAGAAAGATGAAAAGGTTCTTCCAAATGGGATTGCTTGGATTCCTCACTTGGTTGAGACAATCACCGCAGTTGTGTTGAAAGAAGGAACCAAGTCTATTTTTGTTGATAAAAATTTGATCGCAGGCCCAAACCCCAATCAAAGGGGAAAGTTGCTGATTCCTTTTATTGTTGTACTCCAATATCTCTTCGCTGTTAGACCTATTGAGCGAGCAATAAGAAGTGATATATTAAATGAAAGTAGACCATCGTGGGAGACGCATGAGTACTAGCTTATCCAGCCGCAGGTATTGATTTGGCCTCATAAATATCCCGAGCGATCAATATGGTTGTTTGGTGAGTTAATCTGAGTCTTTTTGTCAATGTTGAGATTGGACTGTCTTGTTTTTTTTTAAAGTTTGAATTTTGAGACAACTGAGTTTCTGTAAGCAAATTCATATACGCTACAAGGTTTAGCTACTCGTATTTAGTTAAACAAGAAATTCTTCAATTTTTTCTAGAAGCGGATGATTACTCATCTGCTTCTCACGTTTCGTGAATAGTTAGGACATGATTCTTTTTGTTCCCCAATCCCATCACTCTTCCCAATTGACTATCAAACTTTCAAACTTTGCATGCAAATGTTATAATGTTCATGATCGAGAT

Coding sequence (CDS)

ATGACAGGCGCACACAGCTTCATGGCATCCCAGGTTATCCGGGAGTGGATTGGGATTAACAACTTTGCAATGGCCACACAAGTCAAATTACTTGAGTTGCTGGGAAGACTGAAGCTGGAGAATGTCAGCACCTTGACCATACTTGTAATGGGAAAGGGTGGCGTTGGAAAGTCATCCACTGTGAATTCCATTATTGGTGAAAGAGTTGTTTCTGTTAGTCCTTTTCAGTCAGAAGTGACAAGGCCTGTGATGGTTTCTCGCTCAAGGGCTGGATTTACATTAAACATCATTGACACACCTGGTATCATAGAAGGAGGTTATATCAATGATCAGACACTTGAGATCATAAAACGCTTTCTTTTGAATAAGACCATAGATATTCTTCTCTATGTGGACCGCTTGGATGTATATCGAGTAGATAACTTGGAAAAGCAGGTTATTAAGGCCATAACAAACAGCTTTGGTAAAGCAATATGGAACAGGGCTTTGGTCGTCCTCACTCATGCTCAATTTTCCCCACCTGATGGAGTACCATATGAAGAATTTTTCTCCAGACGATCAGAAGCTCTACTGAAAACCGTCCGATTTGGCGCCTCTTTCAACAAAAAAGATATTCAGGCGTTGGATATTCCAGTTGTTTTGGTTGAGAACAGTGGACGGTGCAATAAGAACGAGAAAGATGAAAAGGTTCTTCCAAATGGGATTGCTTGGATTCCTCACTTGGTTGAGACAATCACCGCAGTTGTGTTGAAAGAAGGAACCAAGTCTATTTTTGTTGATAAAAATTTGATCGCAGGCCCAAACCCCAATCAAAGGGGAAAGTTGCTGATTCCTTTTATTGTTGTACTCCAATATCTCTTCGCTGTTAGACCTATTGAGCGAGCAATAAGAAGTGATATATTAAATGAAAGTAGACCATCGTGGGAGACGCATGAGTACTAG

Protein sequence

MTGAHSFMASQVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDILLYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSEALLKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAVVLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSWETHEY

Homology

[Close]

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot
Match: Q41009 (Translocase of chloroplast 34 OS=Pisum sativum OX=3888 GN=TOC34 PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.7e-129
Identity = 233/302 (77.15%), Postives = 260/302 (86.09%), Query Frame = 0


Query: 11  QVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVV 70
           Q +REW GIN FA ATQ KLLELLG LK E+V++LTILVMGKGGVGKSSTVNSIIGERVV
Sbjct: 6   QTVREWSGINTFAPATQTKLLELLGNLKQEDVNSLTILVMGKGGVGKSSTVNSIIGERVV 65

Query: 71  SVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDILLY 130
           S+SPFQSE  RPVMVSRSRAGFTLNIIDTPG+IEGGYIND  L IIK FLL+KTID+LLY
Sbjct: 66  SISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLIEGGYINDMALNIIKSFLLDKTIDVLLY 125

Query: 131 VDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSEAL 190
           VDRLD YRVDNL+K V KAIT+SFGK IWN+A+V LTHAQFSPPDG+PY+EFFS+RSEAL
Sbjct: 126 VDRLDAYRVDNLDKLVAKAITDSFGKGIWNKAIVALTHAQFSPPDGLPYDEFFSKRSEAL 185

Query: 191 LKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAVVL 250
           L+ VR GAS  KKD QA DIPVVL+ENSGRCNKN+ DEKVLPNGIAWIPHLV+TIT V L
Sbjct: 186 LQVVRSGASL-KKDAQASDIPVVLIENSGRCNKNDSDEKVLPNGIAWIPHLVQTITEVAL 245

Query: 251 KEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSWET 310
            + ++SIFVDKNLI GPNPNQRGKL IP I  LQYLF  +PIE  IR DI  E++P+WET
Sbjct: 246 NK-SESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPIEALIRRDIATETKPAWET 305

Query: 311 HE 313
            +
Sbjct: 306 RD 305

[Close]

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot
Match: Q38906 (Translocase of chloroplast 34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC34 PE=1 SV=2)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-111
Identity = 193/304 (63.49%), Postives = 242/304 (79.61%), Query Frame = 0


Query: 9   ASQVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGER 68
           A Q +REWIGI  F  ATQ KLLE+LG+ K E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 69  VVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDIL 128
             +VS FQSE  RP +VSR+R+GFTLNIIDTPG+IEGGY+NDQ + IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 129 LYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSE 188
           LYVDRLDVYRVD+L++QV+ AIT++FGK IW ++ +VLTHAQFSPPDG+ Y  F S+RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 189 ALLKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAV 248
           ALLK ++ GA   K+D+Q   IPV+LVENSGRC+KNE DEK+LP G +WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 249 VLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSW 308
               G K+I VDK L+ GPNPN+RGK LIP +   QYL  ++P+ RAI+SD+  ES+P+W
Sbjct: 243 SF-NGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAW 302

Query: 309 ETHE 313
           E  +
Sbjct: 303 ELRD 305

[Close]

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot
Match: O23680 (Translocase of chloroplast 33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC33 PE=1 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-96
Identity = 174/295 (58.98%), Postives = 228/295 (77.29%), Query Frame = 0


Query: 12  VIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVVS 71
           ++REW+G   F  ATQ KL+E  G+LK ++++++T+LV+GKGGVGKSSTVNS+IGE+VV 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 72  VSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDILLYV 131
           VSPFQ+E  RPVMVSR+  GFT+NIIDTPG++E GY+N Q LE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 132 DRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSEALL 191
           DRLDVYRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD + YE F S+RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 192 KTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAVVLK 251
           KT+R G+   K++ +   I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V   
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 252 EGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRP 307
           +  K+I VDK ++ G   + +GK LIP I+  QYL  V+ I+ AIR+DI    +P
Sbjct: 244 Q-RKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLI-VKMIQGAIRNDIKTSGKP 296

[Close]

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot
Match: A8HYJ1 (Translocase of chloroplast 34 homolog, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=TOC34 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-33
Identity = 98/286 (34.27%), Postives = 145/286 (50.70%), Query Frame = 0


Query: 16  WIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVVSVSPF 75
           W G+N   +  +  +L++L  L+ E    LT+L++GK  VGKSS +NS++GE VV V  F
Sbjct: 66  WAGLNR--LPERDDMLDILNELRAEGRKQLTVLLLGKSSVGKSSLINSLLGEAVVRVQAF 125

Query: 76  Q--------SEVTRPVMVSRSRA-GFTLNIIDTPGIIE---GGYINDQTLEIIKRFLLNK 135
           +        + V R V V  S   GF L +IDT G+ +   G  +N   L  I   +   
Sbjct: 126 KLQADTDITTTVVRQVAVGNSEVDGFRLKLIDTCGLEDPEAGDTVNLGALSKIAEDVRGV 185

Query: 136 TIDILLYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQF-SPPDGVPYEEF 195
            ID++LY DRLD+YRVD L+K +I AI+++FG+ IW R +V LTHA     P G  Y+ F
Sbjct: 186 GIDVVLYCDRLDLYRVDPLDKAIIDAISSTFGRGIWRRTVVALTHANLVQTPPGTDYDSF 245

Query: 196 FSRRSEALLKTVRFGASFNKKDIQALDIPVVLVENSGRCN-KNEKDEKVLPNGIAWIPHL 255
            + R   +   VR    F         +PV LVENS  C   +E   +VLP+G  W+  L
Sbjct: 246 VNGRVRLIRGAVRGPLFFRP------SLPVALVENSETCPVSSESGFRVLPDGEPWLVAL 305

Query: 256 VETITAVVLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLF 288
           V  +  +         +  +       P+ R + L+P  +  + LF
Sbjct: 306 VSQLVDMAAARRRPYKYHPR---LSSKPSHRFRWLLPVAIAAEVLF 340

[Close]

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot
Match: Q9LUS2 (Translocase of chloroplast 120, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TOC120 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-32
Identity = 93/285 (32.63%), Postives = 148/285 (51.93%), Query Frame = 0


Query: 46  TILVMGKGGVGKSSTVNSIIGERVVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGII-- 105
           TI+V+GK GVGKS+T+NSI  E  +S   FQ    +   +     G  + +IDTPG++  
Sbjct: 458 TIMVLGKSGVGKSATINSIFDELKISTDAFQVGTKKVQDIEGFVQGIKVRVIDTPGLLPS 517

Query: 106 -EGGYINDQTLEIIKRFLLNKTIDILLYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRA 165
               + N++ L+ ++ F+     DI+LY+DRLD+   D+ +  +++ IT+ FG +IW  A
Sbjct: 518 WSDQHKNEKILKSVRAFIKKSPPDIVLYLDRLDMQSRDSGDMPLLRTITDVFGPSIWFNA 577

Query: 166 LVVLTHAQFSPPDG-----VPYEEFFSRRSEALLKTVRFGASFNKKDIQALDIPVVLVEN 225
           +V LTHA  +PPDG       Y+ F ++RS  + + +R  A     D++ ++ PV LVEN
Sbjct: 578 IVGLTHAASAPPDGPNGTASSYDMFVTQRSHVIQQAIRQAAG----DMRLMN-PVSLVEN 637

Query: 226 SGRCNKNEKDEKVLPNGIAWIPH-LVETITAVVLKEGTKSIFVDKNLIAGPNPNQRGKLL 285
              C  N   ++VLPNG  W PH L+ +  + +L E    + +  N+  G    +     
Sbjct: 638 HSACRTNRAGQRVLPNGQVWKPHLLLLSFASKILAEANALLKLQDNIPGGQFATRSKAPP 697

Query: 286 IPFIVVLQYLFAVRPIERAIR--------SDILNESRPSWETHEY 314
           +P  ++L  L   RP  +            D L+ES  S E  EY
Sbjct: 698 LP--LLLSSLLQSRPQAKLPEQQYDDEDDEDDLDESSDSEEESEY 735

[Close]

BLAST of CmaCh16G012380 vs. TAIR 10
Match: AT5G05000.2 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 193/304 (63.49%), Postives = 242/304 (79.61%), Query Frame = 0


Query: 9   ASQVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGER 68
           A Q +REWIGI  F  ATQ KLLE+LG+ K E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 69  VVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDIL 128
             +VS FQSE  RP +VSR+R+GFTLNIIDTPG+IEGGY+NDQ + IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 129 LYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSE 188
           LYVDRLDVYRVD+L++QV+ AIT++FGK IW ++ +VLTHAQFSPPDG+ Y  F S+RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 189 ALLKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAV 248
           ALLK ++ GA   K+D+Q   IPV+LVENSGRC+KNE DEK+LP G +WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 249 VLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSW 308
               G K+I VDK L+ GPNPN+RGK LIP +   QYL  ++P+ RAI+SD+  ES+P+W
Sbjct: 243 SF-NGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAW 302

Query: 309 ETHE 313
           E  +
Sbjct: 303 ELRD 305

[Close]

BLAST of CmaCh16G012380 vs. TAIR 10
Match: AT5G05000.3 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 193/304 (63.49%), Postives = 242/304 (79.61%), Query Frame = 0


Query: 9   ASQVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGER 68
           A Q +REWIGI  F  ATQ KLLE+LG+ K E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 69  VVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDIL 128
             +VS FQSE  RP +VSR+R+GFTLNIIDTPG+IEGGY+NDQ + IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 129 LYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSE 188
           LYVDRLDVYRVD+L++QV+ AIT++FGK IW ++ +VLTHAQFSPPDG+ Y  F S+RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 189 ALLKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAV 248
           ALLK ++ GA   K+D+Q   IPV+LVENSGRC+KNE DEK+LP G +WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 249 VLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSW 308
               G K+I VDK L+ GPNPN+RGK LIP +   QYL  ++P+ RAI+SD+  ES+P+W
Sbjct: 243 SF-NGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAW 302

Query: 309 ETHE 313
           E  +
Sbjct: 303 ELRD 305

[Close]

BLAST of CmaCh16G012380 vs. TAIR 10
Match: AT5G05000.1 (translocon at the outer envelope membrane of chloroplasts 34 )

HSP 1 Score: 404.1 bits (1037), Expect = 1.1e-112
Identity = 193/304 (63.49%), Postives = 242/304 (79.61%), Query Frame = 0


Query: 9   ASQVIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGER 68
           A Q +REWIGI  F  ATQ KLLE+LG+ K E+VS+LT+LVMGKGGVGKSSTVNS+IGE+
Sbjct: 3   ALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEK 62

Query: 69  VVSVSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDIL 128
             +VS FQSE  RP +VSR+R+GFTLNIIDTPG+IEGGY+NDQ + IIKRFLLN TID+L
Sbjct: 63  AAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVL 122

Query: 129 LYVDRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSE 188
           LYVDRLDVYRVD+L++QV+ AIT++FGK IW ++ +VLTHAQFSPPDG+ Y  F S+RS 
Sbjct: 123 LYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSN 182

Query: 189 ALLKTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAV 248
           ALLK ++ GA   K+D+Q   IPV+LVENSGRC+KNE DEK+LP G +WIP+L   IT +
Sbjct: 183 ALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEI 242

Query: 249 VLKEGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRPSW 308
               G K+I VDK L+ GPNPN+RGK LIP +   QYL  ++P+ RAI+SD+  ES+P+W
Sbjct: 243 SF-NGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAW 302

Query: 309 ETHE 313
           E  +
Sbjct: 303 ELRD 305

[Close]

BLAST of CmaCh16G012380 vs. TAIR 10
Match: AT1G02280.1 (translocon at the outer envelope membrane of chloroplasts 33 )

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-97
Identity = 174/295 (58.98%), Postives = 228/295 (77.29%), Query Frame = 0


Query: 12  VIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVVS 71
           ++REW+G   F  ATQ KL+E  G+LK ++++++T+LV+GKGGVGKSSTVNS+IGE+VV 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 72  VSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDILLYV 131
           VSPFQ+E  RPVMVSR+  GFT+NIIDTPG++E GY+N Q LE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 132 DRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSEALL 191
           DRLDVYRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD + YE F S+RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 192 KTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAVVLK 251
           KT+R G+   K++ +   I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V   
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 252 EGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRP 307
           +  K+I VDK ++ G   + +GK LIP I+  QYL  V+ I+ AIR+DI    +P
Sbjct: 244 Q-RKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLI-VKMIQGAIRNDIKTSGKP 296

[Close]

BLAST of CmaCh16G012380 vs. TAIR 10
Match: AT1G02280.2 (translocon at the outer envelope membrane of chloroplasts 33 )

HSP 1 Score: 353.2 bits (905), Expect = 2.1e-97
Identity = 174/295 (58.98%), Postives = 228/295 (77.29%), Query Frame = 0


Query: 12  VIREWIGINNFAMATQVKLLELLGRLKLENVSTLTILVMGKGGVGKSSTVNSIIGERVVS 71
           ++REW+G   F  ATQ KL+E  G+LK ++++++T+LV+GKGGVGKSSTVNS+IGE+VV 
Sbjct: 4   LVREWVGFQQFPAATQEKLIEFFGKLKQKDMNSMTVLVLGKGGVGKSSTVNSLIGEQVVR 63

Query: 72  VSPFQSEVTRPVMVSRSRAGFTLNIIDTPGIIEGGYINDQTLEIIKRFLLNKTIDILLYV 131
           VSPFQ+E  RPVMVSR+  GFT+NIIDTPG++E GY+N Q LE+IK FL+N+TID+LLYV
Sbjct: 64  VSPFQAEGLRPVMVSRTMGGFTINIIDTPGLVEAGYVNHQALELIKGFLVNRTIDVLLYV 123

Query: 132 DRLDVYRVDNLEKQVIKAITNSFGKAIWNRALVVLTHAQFSPPDGVPYEEFFSRRSEALL 191
           DRLDVYRVD L+KQV+ AIT +FGK IW + L+VLTHAQFSPPD + YE F S+RS++LL
Sbjct: 124 DRLDVYRVDELDKQVVIAITQTFGKEIWCKTLLVLTHAQFSPPDELSYETFSSKRSDSLL 183

Query: 192 KTVRFGASFNKKDIQALDIPVVLVENSGRCNKNEKDEKVLPNGIAWIPHLVETITAVVLK 251
           KT+R G+   K++ +   I VV  ENSGRC+KN+KDEK LPNG AWIP+LV+ IT V   
Sbjct: 184 KTIRAGSKMRKQEFEDSAIAVVYAENSGRCSKNDKDEKALPNGEAWIPNLVKAITDVATN 243

Query: 252 EGTKSIFVDKNLIAGPNPNQRGKLLIPFIVVLQYLFAVRPIERAIRSDILNESRP 307
           +  K+I VDK ++ G   + +GK LIP I+  QYL  V+ I+ AIR+DI    +P
Sbjct: 244 Q-RKAIHVDKKMVDGSYSDDKGKKLIPLIIGAQYLI-VKMIQGAIRNDIKTSGKP 296

The following BLAST results are available for this feature:

BLAST of CmaCh16G012380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41009	2.7e-129	77.15	Translocase of chloroplast 34 OS=Pisum sativum OX=3888 GN=TOC34 PE=1 SV=1	[more]
Q38906	1.5e-111	63.49	Translocase of chloroplast 34, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O23680	3.0e-96	58.98	Translocase of chloroplast 33, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A8HYJ1	1.6e-33	34.27	Translocase of chloroplast 34 homolog, chloroplastic OS=Chlamydomonas reinhardti...	[more]
Q9LUS2	1.8e-32	32.63	Translocase of chloroplast 120, chloroplastic OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of CmaCh16G012380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G05000.2	1.1e-112	63.49	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT5G05000.3	1.1e-112	63.49	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT5G05000.1	1.1e-112	63.49	translocon at the outer envelope membrane of chloroplasts 34	[more]
AT1G02280.1	2.1e-97	58.98	translocon at the outer envelope membrane of chloroplasts 33	[more]
AT1G02280.2	2.1e-97	58.98	translocon at the outer envelope membrane of chloroplasts 33	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 8..275 e-value: 1.4E-77 score: 262.7
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 14..246
IPR005688	Chloroplast protein import component Toc34	TIGRFAM	TIGR00991	TIGR00991	coord: 8..311 e-value: 1.4E-164 score: 544.6
IPR005688	Chloroplast protein import component Toc34	PIRSF	PIRSF038134	Toc33/toc34	coord: 8..313 e-value: 1.5E-157 score: 521.2
IPR006703	AIG1-type guanine nucleotide-binding (G) domain	PFAM	PF04548	AIG1	coord: 45..229 e-value: 5.4E-38 score: 130.4
IPR006703	AIG1-type guanine nucleotide-binding (G) domain	PROSITE	PS51720	G_AIG1	coord: 42..267 score: 38.561069
None	No IPR available	PIRSR	PIRSR038134-1	PIRSR038134-1	coord: 13..300 e-value: 1.3E-145 score: 481.6
None	No IPR available	PANTHER	PTHR10903:SF125	TRANSLOCASE OF CHLOROPLAST	coord: 8..308
None	No IPR available	CDD	cd01853	Toc34_like	coord: 14..260 e-value: 1.29257E-125 score: 357.398
IPR045058	GTPase GIMA/IAN/Toc	PANTHER	PTHR10903	GTPASE, IMAP FAMILY MEMBER-RELATED	coord: 8..308

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G012380.1	CmaCh16G012380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0071806	protein transmembrane transport
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0042802	identical protein binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0015450	protein-transporting ATPase activity