CmaCh16G012370 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G012370
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionnaringenin,2-oxoglutarate 3-dioxygenase-like
LocationCma_Chr16: 9362199 .. 9365013 (+)
RNA-Seq ExpressionCmaCh16G012370
SyntenyCmaCh16G012370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGCCGCTTAACTTCCACTCTCACCGATCTTGCCGATGAGCCAACTTTGCAGCCCGATTTCATCCGCGACGAGGATGAGCGTTCCAAAGTTCCTTACAACCATTTCAGCAATGAAGTCCCGATCATCTCCATCGCCGGAATCGACGACGTTGAAGGCGGCGGCAGGGCCGAGATCTGCCGGAAGATCATTGACGCTTGCGAGGACTGGGGGATCTTCCAGATCGTCGACCATGGCGTCGATTCGGAAATTGTTTCCGATATGGCTCGTCTCGCTCGAGAGTTCTTTGCACTTCCGTCCGAGGAGAAACTCCGTTACGATATGTCTGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGCCATCTCAAGGTAATGATATTGAATAAACCTCCTCCGATTCTCCATCATTATGCTTGTGATTTTCAGATCTGTGGTTTTAATTTGTTGAAGTTGAATATCACAACATTTGGATGCATGAAATTTTTTTGAATCATTATAAAATTCAAAAAATAAAAGAACTATGGAAATTAATAAAAATATACAGTTCATAAATTTTATATGAAATTCAAGTTGGTAATATGTTTCATGGATTCATGTCTCGATCTCATGAATATCGAGGAAATCGGCTTATTTAGATAAAAATTAGGGCTAAATTATACGTAAATCAATTTCAAAACTCTAAATCAGATGAGATGGATCATTGACATTAATCGAAAATGAAGGACGTGTAGTATTTAATAGCTGGGTGTTGATCGTACTCATAAACTTTCAAAATCATCTTAGCTTACTTCTAAAATGGTTGCTATGGAGCTAATAGGTTACTGTAAATGGTAAATTGACAGGGAGAAGTTGTTAAAGATTGGCGTGAGATAGTGACCTACTTTTCGTACCCGATTAGAAACAGAGACTATTCGCTGTGGCCAGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGATGATGGGATTGGGGCGTAAGATGTTGGTGGTTTTATCAGAAGCCATGGGGCTGGAGAAGGAGGCCATAGTAAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTGAATTTCTATCCCAAATGCCCTCAGCCGGACCTCACTTTGGGGCTGAAGCGCCACACTGACCCTGGAACCATAACGCTGCTGCTGCAGGACCAGGTCGGTGGGCTTCAGGCCACCAAGGACGGTGGCCGGACGTGGATCACTGTCCAGCCCGTCCAAGGCGCTTTCATCGTCAACTTGGGAGACCATAGCCATGTAAGTCTACTGTTTTTTATTCCTGCCTTTTAAAGTGCTCGCCCATGATTAAAAGTTGCTAATCAACTTTGCACCTTCATCCTTCAAATTTTTAAAATATTTTAGTTTCAAAAATACATACAATTTATTTTTAAATTGGTTCTTTTAATTAACTAACTATTTGAAGGGTAGATAAATCATTTATGTTCATATTTTAACCTTATTCTAATTATAAACCAATTTTAATTTTTTAAAAAAAAAATAAAGTATGTTAATGCTCATTTTAAAAATTTATAAGTATATTTGAAATATTATACTAATTGTTCTTCTGTTATTAGTACTACTAAATATTTTTAAAAGTTTTATAAAAGAAAAAAAAAAGTTTTAAAGATATTAATAAAACTAAAAAAATTTAAAGAATGATAGAAAATAGAATTTCAGGTTATTTATAAATATTATTTAATATGGTTGGATTTCACATCATTCTTAAAATGTTAATGTAACACCCAATTAATGAGAGTAGTACCCACACCATCCTTGAATAAAGATGTGTCTAATTTTGTGTCTAATAACGTAACATCAATTTTGAATTTTGTGTCTGATAATGAAGATCCACCAATCATATGTTGAATAGGGATTATAAACTATTAATTTCATGTCTAATATGTTCGTAAGCTTTATAACTACTAATAATATTGAATATACCTTAGACATAAAATCGAACTTTTAGAAATTTATTAGATAACCTATTAAATAACACTAAAAGTAGTAATTTAACTTTATATATATATATTTAATTTATCGTAACAACTCCAAGAGTGGAGAATTGAGAAACTTAAAATGTATGTGAAATGTGAGGCTTTTATTAATTAATAAATCATTTTAATAAAAGTAAAATTATAATAAAAATTTAGGTTAACAAATAAGGTATCTCTTTATTGTATAGGATGTGGATTCAAATTTTCACTCCCACAAGGGCAAGAGAGGATTAAAAGATTTCCCGCTAGATATAACATTAAAAAGCTTAAAAATACTGATTACAAAGTTTTATTTTTATTTTTATTTTTATTTGAGTAACTTGATTGGGAATACAATCTTATCTTAACCGGTAAAACTATGTTCAAATGACCATATATTATTAAATCGGAAAAATAGACTTGTAATAACGGATTTGGGTGCTCTAGCCAGCTATTACCTTCTTCATTACGAGTAATGAATCTCCTGATCTTGCTTTGACCACGTATTATTGATGGTTGCAACAGTATCTAAGCAATGGGAGGTTCAAGAATGCAGAGCACAGAGCAGTAGTGAACTCAAACTCAAGCAGGCTATCAATAGCGACATTTCAAAACCCTGCGCCGGAGGCTATTGTGTATCCACTGAAGATCAGAGAAGGGGAGAAGCCTATTCTTGAGAAGCCCATCACCTTCAGTGAGATGTACCAGACCAAGATGAACAGGGACATTGAGCTTGCAAAGCTTAAGAAGCAGGCCCAACAACAACAACAACAAGCCCAAAAGCTTGTTGGATAAGAAGGCCCATTTTGGTGGAAGCCCAAGCCC

mRNA sequence

ATGGAAGGCCGCTTAACTTCCACTCTCACCGATCTTGCCGATGAGCCAACTTTGCAGCCCGATTTCATCCGCGACGAGGATGAGCGTTCCAAAGTTCCTTACAACCATTTCAGCAATGAAGTCCCGATCATCTCCATCGCCGGAATCGACGACGTTGAAGGCGGCGGCAGGGCCGAGATCTGCCGGAAGATCATTGACGCTTGCGAGGACTGGGGGATCTTCCAGATCGTCGACCATGGCGTCGATTCGGAAATTGTTTCCGATATGGCTCGTCTCGCTCGAGAGTTCTTTGCACTTCCGTCCGAGGAGAAACTCCGTTACGATATGTCTGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGCCATCTCAAGGGAGAAGTTGTTAAAGATTGGCGTGAGATAGTGACCTACTTTTCGTACCCGATTAGAAACAGAGACTATTCGCTGTGGCCAGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGATGATGGGATTGGGGCGTAAGATGTTGGTGGTTTTATCAGAAGCCATGGGGCTGGAGAAGGAGGCCATAGTAAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTGAATTTCTATCCCAAATGCCCTCAGCCGGACCTCACTTTGGGGCTGAAGCGCCACACTGACCCTGGAACCATAACGCTGCTGCTGCAGGACCAGGTCGGTGGGCTTCAGGCCACCAAGGACGGTGGCCGGACGTGGATCACTGTCCAGCCCGTCCAAGGCGCTTTCATCGTCAACTTGGGAGACCATAGCCATTATCTAAGCAATGGGAGGTTCAAGAATGCAGAGCACAGAGCAGTAGTGAACTCAAACTCAAGCAGGCTATCAATAGCGACATTTCAAAACCCTGCGCCGGAGGCTATTGTGTATCCACTGAAGATCAGAGAAGGGGAGAAGCCTATTCTTGAGAAGCCCATCACCTTCAGTGAGATGTACCAGACCAAGATGAACAGGGACATTGAGCTTGCAAAGCTTAAGAAGCAGGCCCAACAACAACAACAACAAGCCCAAAAGCTTGTTGGATAAGAAGGCCCATTTTGGTGGAAGCCCAAGCCC

Coding sequence (CDS)

ATGGAAGGCCGCTTAACTTCCACTCTCACCGATCTTGCCGATGAGCCAACTTTGCAGCCCGATTTCATCCGCGACGAGGATGAGCGTTCCAAAGTTCCTTACAACCATTTCAGCAATGAAGTCCCGATCATCTCCATCGCCGGAATCGACGACGTTGAAGGCGGCGGCAGGGCCGAGATCTGCCGGAAGATCATTGACGCTTGCGAGGACTGGGGGATCTTCCAGATCGTCGACCATGGCGTCGATTCGGAAATTGTTTCCGATATGGCTCGTCTCGCTCGAGAGTTCTTTGCACTTCCGTCCGAGGAGAAACTCCGTTACGATATGTCTGGCGGTAAGAAGGGCGGATTCATCGTCTCCAGCCATCTCAAGGGAGAAGTTGTTAAAGATTGGCGTGAGATAGTGACCTACTTTTCGTACCCGATTAGAAACAGAGACTATTCGCTGTGGCCAGACAAGCCAGAGGGGTGGGCGGCGAAGACGGCGGAGTACAGCGAGAAGATGATGGGATTGGGGCGTAAGATGTTGGTGGTTTTATCAGAAGCCATGGGGCTGGAGAAGGAGGCCATAGTAAAGGCCTGTGTGGACATGGACCAGAAGATTGTGGTGAATTTCTATCCCAAATGCCCTCAGCCGGACCTCACTTTGGGGCTGAAGCGCCACACTGACCCTGGAACCATAACGCTGCTGCTGCAGGACCAGGTCGGTGGGCTTCAGGCCACCAAGGACGGTGGCCGGACGTGGATCACTGTCCAGCCCGTCCAAGGCGCTTTCATCGTCAACTTGGGAGACCATAGCCATTATCTAAGCAATGGGAGGTTCAAGAATGCAGAGCACAGAGCAGTAGTGAACTCAAACTCAAGCAGGCTATCAATAGCGACATTTCAAAACCCTGCGCCGGAGGCTATTGTGTATCCACTGAAGATCAGAGAAGGGGAGAAGCCTATTCTTGAGAAGCCCATCACCTTCAGTGAGATGTACCAGACCAAGATGAACAGGGACATTGAGCTTGCAAAGCTTAAGAAGCAGGCCCAACAACAACAACAACAAGCCCAAAAGCTTGTTGGATAA

Protein sequence

MEGRLTSTLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIIDACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEKEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRTWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQKLVG
Homology
BLAST of CmaCh16G012370 vs. ExPASy Swiss-Prot
Match: Q07353 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN=AN3 PE=1 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.4e-161
Identity = 275/346 (79.48%), Postives = 312/346 (90.17%), Query Frame = 0

Query: 7   STLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIID 66
           STLT LA+E TLQ  FIRDEDER KV YN FSNE+PIIS+ GIDD E G RAEIC KI+ 
Sbjct: 7   STLTALAEEKTLQTSFIRDEDERPKVAYNQFSNEIPIISLEGIDD-ETGKRAEICDKIVK 66

Query: 67  ACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGE 126
           ACEDWG+FQ+VDHGVD+E++S M   A+EFFALP EEKLR+DMSGGKKGGFIVSSHL+GE
Sbjct: 67  ACEDWGVFQVVDHGVDAEVISQMTTFAKEFFALPPEEKLRFDMSGGKKGGFIVSSHLQGE 126

Query: 127 VVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLE 186
           VV+DWREIVTYFSYP R RDYS WPDKPEGW A T +YSEK+M L  K+L VLSEAMGLE
Sbjct: 127 VVQDWREIVTYFSYPTRARDYSRWPDKPEGWIAVTQKYSEKLMELACKLLDVLSEAMGLE 186

Query: 187 KEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGR 246
           KEA+ KACVDMDQK+VVNFYPKCP+PDLTLGLKRHTDPGTITLLLQDQVGGLQATKD G+
Sbjct: 187 KEALTKACVDMDQKVVVNFYPKCPEPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDNGK 246

Query: 247 TWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYP 306
           TWITVQPV+GAF+VNLGDH H+LSNGRFKNA+H+AVVNSNSSRLSIATFQNPAPEAIVYP
Sbjct: 247 TWITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEAIVYP 306

Query: 307 LKIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQ 353
           LKIREGEK I+++PITF+EMY+ KM++D+ELA+LKKQA++QQ QA+
Sbjct: 307 LKIREGEKSIMDEPITFAEMYRRKMSKDLELARLKKQAKEQQLQAE 351

BLAST of CmaCh16G012370 vs. ExPASy Swiss-Prot
Match: Q06942 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 8.2e-159
Identity = 269/344 (78.20%), Postives = 309/344 (89.83%), Query Frame = 0

Query: 7   STLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIID 66
           +TLT +A E TLQ  F+RDEDER KV YN FSNE+PIIS+AGID+VE G R EIC+KI+ 
Sbjct: 5   TTLTSIAHEKTLQQKFVRDEDERPKVAYNDFSNEIPIISLAGIDEVE-GRRGEICKKIVA 64

Query: 67  ACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGE 126
           ACEDWGIFQIVDHGVD+E++S+M  LAREFFALPSEEKLR+DMSGGKKGGFIVSSHL+GE
Sbjct: 65  ACEDWGIFQIVDHGVDAELISEMTGLAREFFALPSEEKLRFDMSGGKKGGFIVSSHLQGE 124

Query: 127 VVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLE 186
            V+DWREIVTYFSYPIR+RDYS WPDKPE W   T +YS+++MGL  K+L VLSEAMGL+
Sbjct: 125 AVQDWREIVTYFSYPIRHRDYSRWPDKPEAWREVTKKYSDELMGLACKLLGVLSEAMGLD 184

Query: 187 KEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGR 246
            EA+ KACVDMDQK+VVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G+
Sbjct: 185 TEALTKACVDMDQKVVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGK 244

Query: 247 TWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYP 306
           TWITVQPV+GAF+VNLGDH H LSNGRFKNA+H+AVVNSNSSRLSIATFQNPA EAIVYP
Sbjct: 245 TWITVQPVEGAFVVNLGDHGHLLSNGRFKNADHQAVVNSNSSRLSIATFQNPAQEAIVYP 304

Query: 307 LKIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQ 351
           L +REGEKPILE PIT++EMY+ KM++D+ELA+LKK A++QQ Q
Sbjct: 305 LSVREGEKPILEAPITYTEMYKKKMSKDLELARLKKLAKEQQSQ 347

BLAST of CmaCh16G012370 vs. ExPASy Swiss-Prot
Match: Q05965 (Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 GN=FHT PE=2 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 1.8e-158
Identity = 265/345 (76.81%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 8   TLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIIDA 67
           TLT+LA E  L   F+RDEDER KV YN FS+E+P+IS+AGIDDV+ G R EICR+I++A
Sbjct: 4   TLTELAGESKLNSKFVRDEDERPKVAYNEFSDEIPVISLAGIDDVD-GKRGEICREIVEA 63

Query: 68  CEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGEV 127
           CE+WGIFQ+VDHGVD+ +V+DM RLAR+FFALP EEKLR+DMSGGKKGGFIVSSHL+GE 
Sbjct: 64  CENWGIFQVVDHGVDTSLVADMTRLARDFFALPPEEKLRFDMSGGKKGGFIVSSHLQGEA 123

Query: 128 VKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEK 187
           V+DWREIVTYFSYP+RNRDYS WPDKP+GWA  T EYSEK+MGL  K+L VLSEAMGLEK
Sbjct: 124 VQDWREIVTYFSYPVRNRDYSRWPDKPQGWAKVTEEYSEKLMGLACKLLEVLSEAMGLEK 183

Query: 188 EAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 247
           E++  ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G T
Sbjct: 184 ESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDDGNT 243

Query: 248 WITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPL 307
           WITVQPV+GAF+VNLGDH H+LSNGRFKNA+H+AVVNSNSSRLSIATFQNPAPEA VYPL
Sbjct: 244 WITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPEATVYPL 303

Query: 308 KIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQ 353
           K+REGEK I+E+PITF+EMY+ KM RD+ELA+LKK A+++    +
Sbjct: 304 KVREGEKAIMEEPITFAEMYKRKMGRDLELARLKKLAKEEHNHKE 347

BLAST of CmaCh16G012370 vs. ExPASy Swiss-Prot
Match: Q9S818 (Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H PE=1 SV=1)

HSP 1 Score: 555.1 bits (1429), Expect = 5.9e-157
Identity = 262/345 (75.94%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 8   TLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIIDA 67
           TLT+LA E  L   F+RDEDER KV YN FS+E+P+IS+AGIDDV+ G R EICR+I++A
Sbjct: 5   TLTELAGESKLNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDDVD-GKRGEICRQIVEA 64

Query: 68  CEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGEV 127
           CE+WGIFQ+VDHGVD+ +V+DM RLAR+FFALP E+KLR+DMSGGKKGGFIVSSHL+GE 
Sbjct: 65  CENWGIFQVVDHGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEA 124

Query: 128 VKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEK 187
           V+DWREIVTYFSYP+RNRDYS WPDKPEGW   T EYSE++M L  K+L VLSEAMGLEK
Sbjct: 125 VQDWREIVTYFSYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEK 184

Query: 188 EAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 247
           E++  ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G+T
Sbjct: 185 ESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKT 244

Query: 248 WITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPL 307
           WITVQPV+GAF+VNLGDH H+LSNGRFKNA+H+AVVNSNSSRLSIATFQNPAP+A VYPL
Sbjct: 245 WITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPL 304

Query: 308 KIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQ 353
           K+REGEK ILE+PITF+EMY+ KM RD+ELA+LKK A++++   +
Sbjct: 305 KVREGEKAILEEPITFAEMYKRKMGRDLELARLKKLAKEERDHKE 348

BLAST of CmaCh16G012370 vs. ExPASy Swiss-Prot
Match: Q7XZQ7 (Flavanone 3-dioxygenase OS=Petroselinum crispum OX=4043 GN=FHT PE=1 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 3.2e-155
Identity = 257/348 (73.85%), Postives = 309/348 (88.79%), Query Frame = 0

Query: 7   STLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIID 66
           STLT LA E TL   F+RDEDER K+ YN FS+E+P+IS+AGIDD     R++ICRKI++
Sbjct: 4   STLTALAQEKTLNSKFVRDEDERPKIAYNKFSDEIPVISLAGIDDDSVDKRSQICRKIVE 63

Query: 67  ACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGE 126
           ACEDWGIFQ+VDHG+D +++S+M RLAR+FFALP+EEKLR+DM+GGKKGGFIVSSHL+GE
Sbjct: 64  ACEDWGIFQVVDHGIDIDLISEMTRLARQFFALPAEEKLRFDMTGGKKGGFIVSSHLQGE 123

Query: 127 VVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLE 186
            V+DWREIVTYFSYPI+ RDYS WPDKPEGW + T  YS+++M L  K+L VLSEAMGLE
Sbjct: 124 AVQDWREIVTYFSYPIQARDYSRWPDKPEGWRSITEMYSDELMALACKLLEVLSEAMGLE 183

Query: 187 KEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGR 246
           KE + KACVDMDQK++VN+YPKCPQP+LTLGLKRHTDPGTITLLLQDQVGGLQAT+DGG+
Sbjct: 184 KEGLTKACVDMDQKVIVNYYPKCPQPNLTLGLKRHTDPGTITLLLQDQVGGLQATRDGGK 243

Query: 247 TWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYP 306
           TWITVQPV+GAF+VNLGDH HYLSNGRFKNA+H+AVVNSNSSR+SIATFQNPAP A VYP
Sbjct: 244 TWITVQPVEGAFVVNLGDHGHYLSNGRFKNADHQAVVNSNSSRMSIATFQNPAPNATVYP 303

Query: 307 LKIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQKL 355
           LKIREGEK ++E+PITF+EMY+ KM+RDIE+A LKK A+++  Q Q++
Sbjct: 304 LKIREGEKAVMEEPITFAEMYKRKMSRDIEMATLKKLAKEKVLQDQEV 351

BLAST of CmaCh16G012370 vs. TAIR 10
Match: AT3G51240.1 (flavanone 3-hydroxylase )

HSP 1 Score: 555.1 bits (1429), Expect = 4.2e-158
Identity = 262/345 (75.94%), Postives = 308/345 (89.28%), Query Frame = 0

Query: 8   TLTDLADEPTLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIIDA 67
           TLT+LA E  L   F+RDEDER KV YN FS+E+P+IS+AGIDDV+ G R EICR+I++A
Sbjct: 5   TLTELAGESKLNSKFVRDEDERPKVAYNVFSDEIPVISLAGIDDVD-GKRGEICRQIVEA 64

Query: 68  CEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGEV 127
           CE+WGIFQ+VDHGVD+ +V+DM RLAR+FFALP E+KLR+DMSGGKKGGFIVSSHL+GE 
Sbjct: 65  CENWGIFQVVDHGVDTNLVADMTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEA 124

Query: 128 VKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEK 187
           V+DWREIVTYFSYP+RNRDYS WPDKPEGW   T EYSE++M L  K+L VLSEAMGLEK
Sbjct: 125 VQDWREIVTYFSYPVRNRDYSRWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEK 184

Query: 188 EAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRT 247
           E++  ACVDMDQKIVVN+YPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G+T
Sbjct: 185 ESLTNACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKT 244

Query: 248 WITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPL 307
           WITVQPV+GAF+VNLGDH H+LSNGRFKNA+H+AVVNSNSSRLSIATFQNPAP+A VYPL
Sbjct: 245 WITVQPVEGAFVVNLGDHGHFLSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPL 304

Query: 308 KIREGEKPILEKPITFSEMYQTKMNRDIELAKLKKQAQQQQQQAQ 353
           K+REGEK ILE+PITF+EMY+ KM RD+ELA+LKK A++++   +
Sbjct: 305 KVREGEKAILEEPITFAEMYKRKMGRDLELARLKKLAKEERDHKE 348

BLAST of CmaCh16G012370 vs. TAIR 10
Match: AT3G51240.2 (flavanone 3-hydroxylase )

HSP 1 Score: 443.0 bits (1138), Expect = 2.3e-124
Identity = 209/264 (79.17%), Postives = 240/264 (90.91%), Query Frame = 0

Query: 89  MARLAREFFALPSEEKLRYDMSGGKKGGFIVSSHLKGEVVKDWREIVTYFSYPIRNRDYS 148
           M RLAR+FFALP E+KLR+DMSGGKKGGFIVSSHL+GE V+DWREIVTYFSYP+RNRDYS
Sbjct: 1   MTRLARDFFALPPEDKLRFDMSGGKKGGFIVSSHLQGEAVQDWREIVTYFSYPVRNRDYS 60

Query: 149 LWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEKEAIVKACVDMDQKIVVNFYPK 208
            WPDKPEGW   T EYSE++M L  K+L VLSEAMGLEKE++  ACVDMDQKIVVN+YPK
Sbjct: 61  RWPDKPEGWVKVTEEYSERLMSLACKLLEVLSEAMGLEKESLTNACVDMDQKIVVNYYPK 120

Query: 209 CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATKDGGRTWITVQPVQGAFIVNLGDHSHY 268
           CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQAT+D G+TWITVQPV+GAF+VNLGDH H+
Sbjct: 121 CPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATRDNGKTWITVQPVEGAFVVNLGDHGHF 180

Query: 269 LSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPLKIREGEKPILEKPITFSEMYQ 328
           LSNGRFKNA+H+AVVNSNSSRLSIATFQNPAP+A VYPLK+REGEK ILE+PITF+EMY+
Sbjct: 181 LSNGRFKNADHQAVVNSNSSRLSIATFQNPAPDATVYPLKVREGEKAILEEPITFAEMYK 240

Query: 329 TKMNRDIELAKLKKQAQQQQQQAQ 353
            KM RD+ELA+LKK A++++   +
Sbjct: 241 RKMGRDLELARLKKLAKEERDHKE 264

BLAST of CmaCh16G012370 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 206.8 bits (525), Expect = 2.8e-53
Identity = 117/329 (35.56%), Postives = 181/329 (55.02%), Query Frame = 0

Query: 5   LTSTLTDLADEPTLQPDFIRDEDERSKVPYNHFSNE-VPIISIAGIDDVEGGGRAEICRK 64
           L S +  + D   +  +++R   +R K+     S + +P+I    + D+ G  RA+I  +
Sbjct: 7   LVSDIASVVDH--VPSNYVRPVSDRPKMSEVQTSGDSIPLID---LHDLHGPNRADIINQ 66

Query: 65  IIDACEDWGIFQIVDHGVDSEIVSDMARLAREFFALPSEEKLRYDMSGGKKGGFIVSS-H 124
              AC   G FQI +HGV  E +  M   AREFF     E++++  +  KK   + +S +
Sbjct: 67  FAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSFN 126

Query: 125 LKGEVVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEA 184
           +  E V +WR+ +    YPI +   + WP  P  +   TAEY+  +  L   +L  +SE+
Sbjct: 127 VSKEKVSNWRDFLRLHCYPIED-FINEWPSTPISFREVTAEYATSVRALVLTLLEAISES 186

Query: 185 MGLEKEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQATK 244
           +GL K+ +        Q + +N+YP+CPQP+LT GL  H D   IT+LLQD+V GLQ  K
Sbjct: 187 LGLAKDRVSNTIGKHGQHMAINYYPRCPQPELTYGLPGHKDANLITVLLQDEVSGLQVFK 246

Query: 245 DGGRTWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEA 304
           DG   WI V PV   FIVNLGD    +SN ++K+  HRAVVNS+  R+SI TF  P+ +A
Sbjct: 247 DG--KWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDA 306

Query: 305 IVYP---LKIREGEKPILEKPITFSEMYQ 329
           ++ P   L   E + P + +  T++E ++
Sbjct: 307 VISPAQELINEEEDSPAIYRNFTYAEYFE 327

BLAST of CmaCh16G012370 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 6.2e-53
Identity = 113/326 (34.66%), Postives = 188/326 (57.67%), Query Frame = 0

Query: 17  TLQPDFIRDEDERSKVPYNHFSNEVPIISIAGIDDVEGGGRAEICRKIIDACEDWGIFQI 76
           TL  +++R   +R ++       + P+I ++  D      R+ + ++I  AC  +G FQ+
Sbjct: 14  TLPENYVRPISDRPRLSEVSQLEDFPLIDLSSTD------RSFLIQQIHQACARFGFFQV 73

Query: 77  VDHGVDSEIVSDMARLAREFFALPSEEKLR-YDMSGGKKGGFIVSSHLKGEVVKDWREIV 136
           ++HGV+ +I+ +M  +AREFF++  EEK++ Y     K      S ++K E V +WR+ +
Sbjct: 74  INHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYL 133

Query: 137 TYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLSEAMGLEKEAIVKACV 196
               YPI ++  + WP  P  +    ++YS ++  +G K+  ++SE++GLEK+ + K   
Sbjct: 134 RLHCYPI-HKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLG 193

Query: 197 DMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQD-QVGGLQATKDGGRTWITVQPV 256
           +  Q + VN+YP CP+P+LT GL  HTDP  +T+LLQD  V GLQ   DG   W  V P 
Sbjct: 194 EQGQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDG--QWFAVNPH 253

Query: 257 QGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAPEAIVYPLK----IR 316
             AF++N+GD    LSNG +K+  HRAV N+ + RLS+A+F  PA  A++ P K      
Sbjct: 254 PDAFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAE 313

Query: 317 EGEKPILEKPITFSEMYQTKMNRDIE 337
           + E   + K  T++E Y+   +R+++
Sbjct: 314 DDETKPVYKDFTYAEYYKKFWSRNLD 330

BLAST of CmaCh16G012370 vs. TAIR 10
Match: AT4G16330.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.6e-51
Identity = 102/247 (41.30%), Postives = 151/247 (61.13%), Query Frame = 0

Query: 66  DACEDWGIFQIVDHGVDSEIVSDMARLAREFFA-LPSEEKLRY---DMSGGKKG-GFIVS 125
           DAC DWG F +++HGV   ++  M  L   FF   P EEKLRY     S   +G G  + 
Sbjct: 84  DACRDWGAFHVINHGVPIHLLDRMRSLGLSFFQDSPMEEKLRYACDSTSAASEGYGSRML 143

Query: 126 SHLKGEVVKDWREIVTYFSYPIRNRDYSLWPDKPEGWAAKTAEYSEKMMGLGRKMLVVLS 185
              K +VV DWR+   + ++P   R+ S WP  P  +     EY ++M  L + +L ++S
Sbjct: 144 LGAKDDVVLDWRDYFDHHTFPPSRRNPSHWPIHPSDYRQVVGEYGDEMKKLAQMLLGLIS 203

Query: 186 EAMGLEKEAIVKACVDMDQKIVVNFYPKCPQPDLTLGLKRHTDPGTITLLLQDQVGGLQA 245
           E++GL   +I +A  ++ Q I V +YP CPQP+LTLGL+ H+D G ITLL+QD V GLQ 
Sbjct: 204 ESLGLPCSSIEEAVGEIYQNITVTYYPPCPQPELTLGLQSHSDFGAITLLIQDDVEGLQL 263

Query: 246 TKDGGRTWITVQPVQGAFIVNLGDHSHYLSNGRFKNAEHRAVVNSNSSRLSIATFQNPAP 305
            KD    W+TV P+  A ++ + D +  ++NGR+K+A+HRAV N+N +RLS+ATF +P+ 
Sbjct: 264 YKDA--QWLTVPPISDAILILIADQTEIITNGRYKSAQHRAVTNANRARLSVATFHDPSK 323

Query: 306 EAIVYPL 308
            A + P+
Sbjct: 324 TARIAPV 328

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q073531.4e-16179.48Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Petunia hybrida OX=4102 GN... [more]
Q069428.2e-15978.20Naringenin,2-oxoglutarate 3-dioxygenase OS=Malus domestica OX=3750 PE=2 SV=1[more]
Q059651.8e-15876.81Naringenin,2-oxoglutarate 3-dioxygenase (Fragment) OS=Matthiola incana OX=3724 G... [more]
Q9S8185.9e-15775.94Naringenin,2-oxoglutarate 3-dioxygenase OS=Arabidopsis thaliana OX=3702 GN=F3H P... [more]
Q7XZQ73.2e-15573.85Flavanone 3-dioxygenase OS=Petroselinum crispum OX=4043 GN=FHT PE=1 SV=1[more]
Match NameE-valueIdentityDescription
AT3G51240.14.2e-15875.94flavanone 3-hydroxylase [more]
AT3G51240.22.3e-12479.17flavanone 3-hydroxylase [more]
AT4G10490.12.8e-5335.562-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.16.2e-5334.662-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G16330.22.6e-5141.302-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 330..356
NoneNo IPR availablePANTHERPTHR47990:SF11NARINGENIN,2-OXOGLUTARATE 3-DIOXYGENASEcoord: 11..347
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 11..347
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 14..333
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 41..152
e-value: 9.7E-21
score: 74.7
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 8..337
e-value: 5.0E-87
score: 294.3
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 199..297
e-value: 3.0E-25
score: 88.7
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 194..298
score: 13.976838

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G012370.1CmaCh16G012370.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding