CmaCh16G012010 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G012010
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Phosphoribulokinase
Location	Cma_Chr16: 9129085 .. 9132389 (+)
RNA-Seq Expression	CmaCh16G012010
Synteny	CmaCh16G012010

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACAATCTCTACAGCCAAGCCCCTAAACTACACAAATTCAGTCTTCTCTTCATCAAGAACAAGCTTAGGACGTCACCAAGAACAGGTAATTTTCTTCACAACAGGCAAACAAGCCAGCAAGAGAGGCAAAGCCAGCACAACCCCATGGCTGATAACCTGCTCAGCAGGCGACCAACAGACAGTTGTGATCGGTCTAGCAGCAGATTCAGGGTGTGGGAAGAGCACTTTCATGAGGAGGCTAACCAGTGTGTTTGGAGGTGCAGCAAAGCCACCAAGAGGTGGAAATCCAGACTCCAATACACTCATAAGTGACACTACAACTGTGATATGTTTAGATGATTACCATTCGTTGGATAGAACTGGAAGAAAAGAGAAGGGAGTTACTGCACTTGACCCAAGAGCCAATGATTTTGATCTCATGTATGAGCAAGTTAAGGCTCTCAAAGATGGAATTGCAGTTGAGAAGCCTATTTATAATCATGTCACTGGTCTTCTTGATCCACCTGAGCTTATTAAGCCCCCTAAGATCCTTGTCATTGAAGGCCTACACCCAATGTAAGTTATTTATGGAATCCAATACGTCGTCTACGAGGTCTATGAACTGCTGCTTAGTCAATTAGTTATGAAATACAGATAACTCTAGGCGTGTTCTCAATATCTTTGTGTTGAGACATAAACCGTTAAAATATCATCAACCAATAAAGTCATTCCCAATAATCGCATGAGACAATTGTTTGTGCATTTTAGGAATAATAAATGGTTAGATATTTTCGGAATAAATTATCTGGCCTTTGCTATAAATACCCCTTCACCCTACCAATGTTTTCATCCCGAAAGAAATACAGATCAAAGTGTAGTAGTATTCTACAGAGTATCTTGTAATCTCTTCACGATTGGTCTTAACAAAGAGTACGTATTTCCCACAAAGAGTTGTGTTCAACATAAATTTCTCCAATCTTTTTTTTCTTTCTTTCTTGAGAAAAGGGGTGAAATGAATTGAATAGATATATTCATTTTTACAACTTCCTTGTTCTTCGTCCTGCATTCATTTCTTACGAAGGGATCAATTGAAGTGAAGAATTTTCAACTCTTTGCATTTTCATATGTTTTTTCTTATTTTTATATATCTCTGTTCATCCAAATCTAGCTTTCCGCCTACTTATGAAGGGATTATTTGAAACGAAGAGTGTTTTCCCCTTCGCATCTTCGAATGTTTTCTTCTCAATCAAACCATTCCATTTCCACACTAGAGAGGGAAAAGAATTCGCACTAAAATTTTATGAAATTTCATGCATTAGAGCTATGTTTATGCAGGTATGATTCACGAGTTAGGGATCTTTTAGACTTCAGCATTTACTTGGATATCAGCAACGAGGTCAAATTCGCTTGGAAGATTCAGGTATGCATTGATCAAATACAATATGAATTAGTAGGCAGAGTATTCTAAATCACATCTCCAATTAGAAATTGAAATCAAAACGATTGCAGAGAGACATGGCAGAGAGAGGTCACAGCCTCGAGAGTATCAAAGCAAGCATCGAAGCGCGAAAGCCAGATTTCGACGCGTATATCGGTAATAATTAAGCTTCAGAAATTCAAAATCCGCCATAGCTAACAAGATTCAAGAAAGAAATTAAGAAAAACTTACTTGAATCTGAATGCGATTCTTCGATTTTTGTTGTCGGATTTAGATCCGCAGAAGCAATTCGCCGATGCGGTGATCGAAGTGCTGCCGACGCGATTGATTCCAAATGACTACGAAGGGAAAATTCTTAGGGTTAGATTGATAATGAGAGAAGGAATCGAGCATTTCAATCCAGTTTACCTGTTCGATGAAGGCTCTACGATCTCGTGGATTCCTTGTGGGAGAAAGCTTACATGTTCTTATCCTGGAATCAGACTATCCTGTGGCCCTGAAACTTACTTCGGTCATGAGGTAATCTTCCTAACTCATGCATTTCATGCAACAACAAGGGATTTTTTGCTCTCCTTTTTCAGGGGTAAATTAGTAAGTTCATGTTTAAATTTTGGACAAAACTTTAAAAATAAATTAATTTTTTAGGTTGGTATCTCTCAACCTTAAAAAAAAAAATCTTATAAATTTATTTTTTTTAATTTATAAATAATAAATTATTAAAATTTCAAATTTATGGTTTTTTAAAAAATTTATGGTTAGGGTTTTGTTAGTCCTAAGAATTAATTTTATTTCTAATATATTAATTAATTTTTAAAATTTTTAATTATCAAAGATTTATTAAAGACAAAATTTTAAATTTTAAAGTTTAACTTAGACTTAAGAAATATATATATATATATATTTATTTATTTATTTATTAATTAAGTAACAATTTATTTTATGCCAAATACTTTGATTATCATATTTTAAAATTTGTATCGATTTAATTAATTGTAGAATATATTGTTAAAATTTAATGAGATGTCTTCGATAATTTGTTCGACGAATTAGCTTAGAGTGTTTTAAAGAAAAAAAAATTAAGGTCAAATGTGTTTATAAATTACTAAAAAAAAAAAAAAAATTGTTGCATTAAAATTGATATACTTAATTTTGTTGAGAATTTTTAAAGTAGGATTAAATTATTATAAATTTTAAAATACAAGGACTAAATTGTTATATAATAAAGTTTAAAACTCGGAAGAATTACAAGGTTTTTCATTAGTCACCCTTGTAAATTTATTTGGATGAAAACCCAACACAAACCAACTCGTGGGCCCGTACGATATCGTAAATTCACTGATAACGGTCGGTTTTGATGGTAATTTTCAGGTAACTGTATTGGAGATGGATGGGCAATTTGATAAATTGGATGAGCTTATATATGTAGAGAGCCATCTAAGCAACATTTCCACCAAATTTTATGGTGAAATTACACAACAAATATTGGAGCATTCTGAATTTCCAGGAAGCAATAATGGAACAGGCCTTTTTCAGACCATCATTGGCTTGAAAATTAGAGACATTTATCACCAAATTATAGCCTCCAAACAACTATCTCAAACTCAATCAGCAAAGGCTTAAAAAGTCGACCCGGTTTGTGTGAGTCGAGAAATTGAATTTTTTAGTTCAACAATGGTCGGGTGGAGGATGGAACCGTATCGATTGTTGGAACGGTAGTTAGTAGTTTATCTACTGAACTATTCTTGAATTGGTTTGGTTTTGGTCATAGTTAAAAATAAGTTTTTTTTCAACTTATTTAGAATGATCTTCGCTCTCATGAACAAGTTGCTTCTTGTATTGGAGATTTTTGTTTCTCAACTTTAATCTCGGCTAATTCTTCTTCC

mRNA sequence

ATGGCAACAATCTCTACAGCCAAGCCCCTAAACTACACAAATTCAGTCTTCTCTTCATCAAGAACAAGCTTAGGACGTCACCAAGAACAGGTAATTTTCTTCACAACAGGCAAACAAGCCAGCAAGAGAGGCAAAGCCAGCACAACCCCATGGCTGATAACCTGCTCAGCAGGCGACCAACAGACAGTTGTGATCGGTCTAGCAGCAGATTCAGGGTGTGGGAAGAGCACTTTCATGAGGAGGCTAACCAGTGTGTTTGGAGGTGCAGCAAAGCCACCAAGAGGTGGAAATCCAGACTCCAATACACTCATAAGTGACACTACAACTGTGATATGTTTAGATGATTACCATTCGTTGGATAGAACTGGAAGAAAAGAGAAGGGAGTTACTGCACTTGACCCAAGAGCCAATGATTTTGATCTCATGTATGAGCAAGTTAAGGCTCTCAAAGATGGAATTGCAGTTGAGAAGCCTATTTATAATCATGTCACTGGTCTTCTTGATCCACCTGAGCTTATTAAGCCCCCTAAGATCCTTGTCATTGAAGGCCTACACCCAATGTATGATTCACGAGTTAGGGATCTTTTAGACTTCAGCATTTACTTGGATATCAGCAACGAGGTCAAATTCGCTTGGAAGATTCAGAGAGACATGGCAGAGAGAGGTCACAGCCTCGAGAGTATCAAAGCAAGCATCGAAGCGCGAAAGCCAGATTTCGACGCGTATATCGATCCGCAGAAGCAATTCGCCGATGCGGTGATCGAAGTGCTGCCGACGCGATTGATTCCAAATGACTACGAAGGGAAAATTCTTAGGGTTAGATTGATAATGAGAGAAGGAATCGAGCATTTCAATCCAGTTTACCTGTTCGATGAAGGCTCTACGATCTCGTGGATTCCTTGTGGGAGAAAGCTTACATGTTCTTATCCTGGAATCAGACTATCCTGTGGCCCTGAAACTTACTTCGGTCATGAGGTAACTGTATTGGAGATGGATGGGCAATTTGATAAATTGGATGAGCTTATATATGTAGAGAGCCATCTAAGCAACATTTCCACCAAATTTTATGGTGAAATTACACAACAAATATTGGAGCATTCTGAATTTCCAGGAAGCAATAATGGAACAGGCCTTTTTCAGACCATCATTGGCTTGAAAATTAGAGACATTTATCACCAAATTATAGCCTCCAAACAACTATCTCAAACTCAATCAGCAAAGGCTTAAAAAGTCGACCCGGTTTGTGTGAGTCGAGAAATTGAATTTTTTAGTTCAACAATGGTCGGGTGGAGGATGGAACCGTATCGATTGTTGGAACGGTAGTTAGTAGTTTATCTACTGAACTATTCTTGAATTGGTTTGGTTTTGGTCATAGTTAAAAATAAGTTTTTTTTCAACTTATTTAGAATGATCTTCGCTCTCATGAACAAGTTGCTTCTTGTATTGGAGATTTTTGTTTCTCAACTTTAATCTCGGCTAATTCTTCTTCC

Coding sequence (CDS)

ATGGCAACAATCTCTACAGCCAAGCCCCTAAACTACACAAATTCAGTCTTCTCTTCATCAAGAACAAGCTTAGGACGTCACCAAGAACAGGTAATTTTCTTCACAACAGGCAAACAAGCCAGCAAGAGAGGCAAAGCCAGCACAACCCCATGGCTGATAACCTGCTCAGCAGGCGACCAACAGACAGTTGTGATCGGTCTAGCAGCAGATTCAGGGTGTGGGAAGAGCACTTTCATGAGGAGGCTAACCAGTGTGTTTGGAGGTGCAGCAAAGCCACCAAGAGGTGGAAATCCAGACTCCAATACACTCATAAGTGACACTACAACTGTGATATGTTTAGATGATTACCATTCGTTGGATAGAACTGGAAGAAAAGAGAAGGGAGTTACTGCACTTGACCCAAGAGCCAATGATTTTGATCTCATGTATGAGCAAGTTAAGGCTCTCAAAGATGGAATTGCAGTTGAGAAGCCTATTTATAATCATGTCACTGGTCTTCTTGATCCACCTGAGCTTATTAAGCCCCCTAAGATCCTTGTCATTGAAGGCCTACACCCAATGTATGATTCACGAGTTAGGGATCTTTTAGACTTCAGCATTTACTTGGATATCAGCAACGAGGTCAAATTCGCTTGGAAGATTCAGAGAGACATGGCAGAGAGAGGTCACAGCCTCGAGAGTATCAAAGCAAGCATCGAAGCGCGAAAGCCAGATTTCGACGCGTATATCGATCCGCAGAAGCAATTCGCCGATGCGGTGATCGAAGTGCTGCCGACGCGATTGATTCCAAATGACTACGAAGGGAAAATTCTTAGGGTTAGATTGATAATGAGAGAAGGAATCGAGCATTTCAATCCAGTTTACCTGTTCGATGAAGGCTCTACGATCTCGTGGATTCCTTGTGGGAGAAAGCTTACATGTTCTTATCCTGGAATCAGACTATCCTGTGGCCCTGAAACTTACTTCGGTCATGAGGTAACTGTATTGGAGATGGATGGGCAATTTGATAAATTGGATGAGCTTATATATGTAGAGAGCCATCTAAGCAACATTTCCACCAAATTTTATGGTGAAATTACACAACAAATATTGGAGCATTCTGAATTTCCAGGAAGCAATAATGGAACAGGCCTTTTTCAGACCATCATTGGCTTGAAAATTAGAGACATTTATCACCAAATTATAGCCTCCAAACAACTATCTCAAACTCAATCAGCAAAGGCTTAA

Protein sequence

MATISTAKPLNYTNSVFSSSRTSLGRHQEQVIFFTTGKQASKRGKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPRANDFDLMYEQVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQFADAVIEVLPTRLIPNDYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIPCGRKLTCSYPGIRLSCGPETYFGHEVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEITQQILEHSEFPGSNNGTGLFQTIIGLKIRDIYHQIIASKQLSQTQSAKA

Homology

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BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot
Match: P27774 (Phosphoribulokinase, chloroplastic OS=Mesembryanthemum crystallinum OX=3544 PE=2 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 1.4e-189
Identity = 323/395 (81.77%), Postives = 360/395 (91.14%), Query Frame = 0


Query: 15  SVFSSSRTS-LGRHQEQVIFFTTGKQASKRGKASTTPWLITCSAGDQQTVVIGLAADSGC 74
           S ++   TS LG +Q++ +FF   K A KR   S+ P +ITC AGD QT+VIGLAADSGC
Sbjct: 4   SAYTVPTTSHLGFNQKKQLFF-CNKSAYKRVSFSSRPCVITCLAGDSQTIVIGLAADSGC 63

Query: 75  GKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALD 134
           GKSTFMRRLTSVFGGAA+PPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALD
Sbjct: 64  GKSTFMRRLTSVFGGAAEPPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALD 123

Query: 135 PRANDFDLMYEQVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILVIEGLHPMYDSRVR 194
           PRANDFDLMYEQVKALK+G AVEKPIYNHVTGLLD PELIKPPKILVIEGLHPM+DSRVR
Sbjct: 124 PRANDFDLMYEQVKALKEGKAVEKPIYNHVTGLLDAPELIKPPKILVIEGLHPMFDSRVR 183

Query: 195 DLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQFADAV 254
           DLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQ+ADAV
Sbjct: 184 DLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQYADAV 243

Query: 255 IEVLPTRLIPNDYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIPCGRKLTCSYPGIR 314
           IEVLPT+LIP D EGK+LRVRLI +EG+++F+PVYLFDEGS+I+WIPCGRKLTCSYPGI+
Sbjct: 244 IEVLPTQLIPGDNEGKVLRVRLIQKEGVQYFSPVYLFDEGSSITWIPCGRKLTCSYPGIK 303

Query: 315 LSCGPETYFGHEVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEITQQILEHSEFPGSN 374
              GP+TYFG+EVTVLEMDGQFD+LDELIYVESHLSN+STKFYGE+TQQ+L+H +FPGSN
Sbjct: 304 FFYGPDTYFGNEVTVLEMDGQFDRLDELIYVESHLSNLSTKFYGEVTQQMLKHQDFPGSN 363

Query: 375 NGTGLFQTIIGLKIRDIYHQIIASKQLSQTQSAKA 409
           NGTGLFQTI+GLKIRD++ Q+IASK  +   + KA
Sbjct: 364 NGTGLFQTIVGLKIRDLFEQLIASKTAAPAAATKA 397

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BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot
Match: P26302 (Phosphoribulokinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.5e-183
Identity = 307/383 (80.16%), Postives = 346/383 (90.34%), Query Frame = 0


Query: 25  GRHQEQVIFFTTGKQASKRGKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTS 84
           G  Q QVIFFTT        +     + ++C+   +Q +VIGLAADSGCGKSTFMRRLTS
Sbjct: 23  GFRQNQVIFFTTRSSRRSNTRHGARTFQVSCAV--EQPIVIGLAADSGCGKSTFMRRLTS 82

Query: 85  VFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPRANDFDLMYE 144
           VFGGAA+PP+GGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDP+ANDFDLMYE
Sbjct: 83  VFGGAAEPPKGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPKANDFDLMYE 142

Query: 145 QVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDI 204
           QVKA+K+G A+EKPIYNHVTGLLDP ELI+PPKI VIEGLHPMYD RVR+LLDFSIYLDI
Sbjct: 143 QVKAIKEGKAIEKPIYNHVTGLLDPAELIQPPKIFVIEGLHPMYDERVRELLDFSIYLDI 202

Query: 205 SNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQFADAVIEVLPTRLIPN 264
           SNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDA+IDPQKQ+ADAVIEVLPT+LIP+
Sbjct: 203 SNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAFIDPQKQYADAVIEVLPTQLIPD 262

Query: 265 DYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIPCGRKLTCSYPGIRLSCGPETYFGH 324
           D EGK+LRV+LIM+EGI+ FNPVYLFDEGSTI+WIPCGRKLTCSYPGI+ S GP+TYFG 
Sbjct: 263 DNEGKVLRVKLIMKEGIKFFNPVYLFDEGSTINWIPCGRKLTCSYPGIKFSYGPDTYFGQ 322

Query: 325 EVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEITQQILEHSEFPGSNNGTGLFQTIIG 384
           EV+VLEMDGQFD+LDELIYVESHLSN+STKFYGE+TQQ+L+H++FPGSNNGTGLFQTI+G
Sbjct: 323 EVSVLEMDGQFDRLDELIYVESHLSNLSTKFYGEVTQQMLKHADFPGSNNGTGLFQTIVG 382

Query: 385 LKIRDIYHQIIASKQLSQTQSAK 408
           LKIRD+Y QIIA +     ++AK
Sbjct: 383 LKIRDLYEQIIAERAGVPAEAAK 403

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BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot
Match: P25697 (Phosphoribulokinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g32060 PE=1 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.1e-182
Identity = 313/405 (77.28%), Postives = 361/405 (89.14%), Query Frame = 0


Query: 1   MATISTAKPLNYTNSVFSSSRTSLGRHQEQVIFFTTGKQASKRGKASTTPWLITCSAGDQ 60
           ++TI + + LN T+ + SSS +      +QV  +    Q ++R        LITC+   Q
Sbjct: 3   VSTIYSTQALNSTHFLTSSSSS------KQVFLYRRQPQTNRRFNT-----LITCA---Q 62

Query: 61  QTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLD 120
           +T+VIGLAADSGCGKSTFMRRLTSVFGGAAKPP+GGNPDSNTLISDTTTVICLDDYHSLD
Sbjct: 63  ETIVIGLAADSGCGKSTFMRRLTSVFGGAAKPPKGGNPDSNTLISDTTTVICLDDYHSLD 122

Query: 121 RTGRKEKGVTALDPRANDFDLMYEQVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILV 180
           R GRKE+ VTALDPRANDFDLMYEQVKALK+GIAVEKPIYNHVTGLLDPPELI+PPKILV
Sbjct: 123 RYGRKEQKVTALDPRANDFDLMYEQVKALKNGIAVEKPIYNHVTGLLDPPELIQPPKILV 182

Query: 181 IEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD 240
           IEGLHPM+D RVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD
Sbjct: 183 IEGLHPMFDERVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD 242

Query: 241 AYIDPQKQFADAVIEVLPTRLIPNDYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIP 300
           A+IDPQKQ+ADAVIEVLPT LIP+D EGK+LRVRLIM+EG+++F+PVYLFDEGSTISWIP
Sbjct: 243 AFIDPQKQYADAVIEVLPTTLIPDDNEGKVLRVRLIMKEGVKYFSPVYLFDEGSTISWIP 302

Query: 301 CGRKLTCSYPGIRLSCGPETYFGHEVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEIT 360
           CGRKLTCSYPGI+ +  P++YF HEV+VLEMDGQFD+LDELIYVESHLSN+STKFYGE+T
Sbjct: 303 CGRKLTCSYPGIKFNYEPDSYFDHEVSVLEMDGQFDRLDELIYVESHLSNLSTKFYGEVT 362

Query: 361 QQILEHSEFPGSNNGTGLFQTIIGLKIRDIYHQIIASKQLSQTQS 406
           QQ+L+H++FPGSNNGTGLFQTI+GLKIRD+Y Q+IA+K  ++ ++
Sbjct: 363 QQMLKHADFPGSNNGTGLFQTIVGLKIRDLYEQLIANKATARAEA 393

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BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot
Match: P09559 (Phosphoribulokinase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 5.3e-178
Identity = 302/398 (75.88%), Postives = 348/398 (87.44%), Query Frame = 0


Query: 12  YTNSVFSSSRTSLGRHQEQVIFFTTGKQASKRGKASTTP-WLITCSAGDQQTVVIGLAAD 71
           YT    +   +S  ++ +QV F      +S     +T P ++ITCS   QQT+VIGLAAD
Sbjct: 7   YTIPTTTHLGSSFNQNNKQVFFNYKRSSSSNNTLFTTRPSYVITCS--QQQTIVIGLAAD 66

Query: 72  SGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLDRTGRKEKGVT 131
           SGCGKSTFMRRLTSVFGGAA+PP+GGNPDSNTLISDTTTVICLDD+HSLDR GRK + VT
Sbjct: 67  SGCGKSTFMRRLTSVFGGAAEPPKGGNPDSNTLISDTTTVICLDDFHSLDRNGRKVEKVT 126

Query: 132 ALDPRANDFDLMYEQVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILVIEGLHPMYDS 191
           ALDP+ANDFDLMYEQVKALK+G AV+KPIYNHV+GLLDPPELI+PPKILVIEGLHPMYD+
Sbjct: 127 ALDPKANDFDLMYEQVKALKEGKAVDKPIYNHVSGLLDPPELIQPPKILVIEGLHPMYDA 186

Query: 192 RVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFDAYIDPQKQFA 251
           RVR+LLDFSIYLDISNEVKFAWKIQRDM ERGHSLESIKASIE+RKPDFDAYIDPQKQ A
Sbjct: 187 RVRELLDFSIYLDISNEVKFAWKIQRDMKERGHSLESIKASIESRKPDFDAYIDPQKQHA 246

Query: 252 DAVIEVLPTRLIPNDYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIPCGRKLTCSYP 311
           D VIEVLPT LIP+D EGK+LRVR+I +EG++ FNPVYLFDEGSTISWIPCGRKLTCSYP
Sbjct: 247 DVVIEVLPTELIPDDDEGKVLRVRMIQKEGVKFFNPVYLFDEGSTISWIPCGRKLTCSYP 306

Query: 312 GIRLSCGPETYFGHEVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEITQQILEHSEFP 371
           GI+ S GP+T++G+EVTV+EMDG FD+LDELIYVESHLSN+STKFYGE+TQQ+L+H  FP
Sbjct: 307 GIKFSYGPDTFYGNEVTVVEMDGMFDRLDELIYVESHLSNLSTKFYGEVTQQMLKHQNFP 366

Query: 372 GSNNGTGLFQTIIGLKIRDIYHQIIASKQLSQTQSAKA 409
           GSNNGTG FQTIIGLKIRD++ Q++AS+  +   +AKA
Sbjct: 367 GSNNGTGFFQTIIGLKIRDLFEQLVASRSTATATAAKA 402

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BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot
Match: P19824 (Phosphoribulokinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=PRKA PE=1 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 2.8e-155
Identity = 262/356 (73.60%), Postives = 307/356 (86.24%), Query Frame = 0


Query: 40  ASKRGKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPD 99
           A  R  A+     +   A   +TVVIGLAADSGCGKSTFMRR+TS+FGG  KPP GGNPD
Sbjct: 14  AQSRVTANRARRSLVVRADKDKTVVIGLAADSGCGKSTFMRRMTSIFGGVPKPPAGGNPD 73

Query: 100 SNTLISDTTTVICLDDYHSLDRTGRKEKGVTALDPRANDFDLMYEQVKALKDGIAVEKPI 159
           SNTLISD TTVICLDDYH LDR GRK KGVTAL P A +FDLMY QVKALK+G +V+KPI
Sbjct: 74  SNTLISDMTTVICLDDYHCLDRNGRKVKGVTALAPEAQNFDLMYNQVKALKEGKSVDKPI 133

Query: 160 YNHVTGLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMA 219
           YNHV+GL+D PE I+ P ILVIEGLHP YD RV +LLDF IYLDIS+++KFAWKIQRDMA
Sbjct: 134 YNHVSGLIDAPEKIESPPILVIEGLHPFYDKRVAELLDFKIYLDISDDIKFAWKIQRDMA 193

Query: 220 ERGHSLESIKASIEARKPDFDAYIDPQKQFADAVIEVLPTRLIPNDYEGKILRVRLIMRE 279
           ERGHSLESIK+SI ARKPDFDAYIDPQK+ AD +I+VLPT+L+P+D +G+ LRVRLIM+E
Sbjct: 194 ERGHSLESIKSSIAARKPDFDAYIDPQKKDADMIIQVLPTQLVPDD-KGQYLRVRLIMKE 253

Query: 280 GIEHFNPVYLFDEGSTISWIPCGRKLTCSYPGIRLSCGPETYFGHEVTVLEMDGQFDKLD 339
           G + F+PVYLFDEGSTISWIPCGRKLTCS+PGI++  GP+T++G EV+VLEMDGQFDKL+
Sbjct: 254 GSKMFDPVYLFDEGSTISWIPCGRKLTCSFPGIKMFYGPDTWYGQEVSVLEMDGQFDKLE 313

Query: 340 ELIYVESHLSNISTKFYGEITQQILEHSEFPGSNNGTGLFQTIIGLKIRDIYHQII 396
           ELIYVESHLSN S KFYGEITQQ+L++S FPGSNNGTGLFQTI+GLK+R++Y +I+
Sbjct: 314 ELIYVESHLSNTSAKFYGEITQQMLKNSGFPGSNNGTGLFQTIVGLKVREVYERIV 368

[Close]

BLAST of CmaCh16G012010 vs. TAIR 10
Match: AT1G32060.1 (phosphoribulokinase )

HSP 1 Score: 639.8 bits (1649), Expect = 1.5e-183
Identity = 313/405 (77.28%), Postives = 361/405 (89.14%), Query Frame = 0


Query: 1   MATISTAKPLNYTNSVFSSSRTSLGRHQEQVIFFTTGKQASKRGKASTTPWLITCSAGDQ 60
           ++TI + + LN T+ + SSS +      +QV  +    Q ++R        LITC+   Q
Sbjct: 3   VSTIYSTQALNSTHFLTSSSSS------KQVFLYRRQPQTNRRFNT-----LITCA---Q 62

Query: 61  QTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDDYHSLD 120
           +T+VIGLAADSGCGKSTFMRRLTSVFGGAAKPP+GGNPDSNTLISDTTTVICLDDYHSLD
Sbjct: 63  ETIVIGLAADSGCGKSTFMRRLTSVFGGAAKPPKGGNPDSNTLISDTTTVICLDDYHSLD 122

Query: 121 RTGRKEKGVTALDPRANDFDLMYEQVKALKDGIAVEKPIYNHVTGLLDPPELIKPPKILV 180
           R GRKE+ VTALDPRANDFDLMYEQVKALK+GIAVEKPIYNHVTGLLDPPELI+PPKILV
Sbjct: 123 RYGRKEQKVTALDPRANDFDLMYEQVKALKNGIAVEKPIYNHVTGLLDPPELIQPPKILV 182

Query: 181 IEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD 240
           IEGLHPM+D RVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD
Sbjct: 183 IEGLHPMFDERVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESIKASIEARKPDFD 242

Query: 241 AYIDPQKQFADAVIEVLPTRLIPNDYEGKILRVRLIMREGIEHFNPVYLFDEGSTISWIP 300
           A+IDPQKQ+ADAVIEVLPT LIP+D EGK+LRVRLIM+EG+++F+PVYLFDEGSTISWIP
Sbjct: 243 AFIDPQKQYADAVIEVLPTTLIPDDNEGKVLRVRLIMKEGVKYFSPVYLFDEGSTISWIP 302

Query: 301 CGRKLTCSYPGIRLSCGPETYFGHEVTVLEMDGQFDKLDELIYVESHLSNISTKFYGEIT 360
           CGRKLTCSYPGI+ +  P++YF HEV+VLEMDGQFD+LDELIYVESHLSN+STKFYGE+T
Sbjct: 303 CGRKLTCSYPGIKFNYEPDSYFDHEVSVLEMDGQFDRLDELIYVESHLSNLSTKFYGEVT 362

Query: 361 QQILEHSEFPGSNNGTGLFQTIIGLKIRDIYHQIIASKQLSQTQS 406
           QQ+L+H++FPGSNNGTGLFQTI+GLKIRD+Y Q+IA+K  ++ ++
Sbjct: 363 QQMLKHADFPGSNNGTGLFQTIVGLKIRDLYEQLIANKATARAEA 393

[Close]

BLAST of CmaCh16G012010 vs. TAIR 10
Match: AT3G27440.1 (uridine kinase-like 5 )

HSP 1 Score: 79.3 bits (194), Expect = 7.7e-15
Identity = 53/200 (26.50%), Postives = 98/200 (49.00%), Query Frame = 0


Query: 60  QQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTLISDTTTVICLDD--YH 119
           +Q  VIG+A  +  GK+T    + S                   + D   V+   D  YH
Sbjct: 27  KQPFVIGVAGGTASGKTTVCNMIMS------------------QLHDQRVVLVNQDSFYH 86

Query: 120 SLDRTGRKEKGVTALD-PRANDFDLMYEQVKALKDGIAVEKPIYN-HVTGLLDPPELIKP 179
           SL +    +      D P A + +++   ++ L+ G  V  P Y+  +   ++    + P
Sbjct: 87  SLTKEKLNKVHEYNFDHPDAFNTEVLLSCMEKLRSGQPVNIPSYDFKIHQSIESSSPVNP 146

Query: 180 PKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDMAERGHSLESI-KASIEA 239
             ++++EG+  + D RVRDL++  I++D   +V+ + +IQRD  ERG +++++ +   + 
Sbjct: 147 GDVIILEGILVLNDPRVRDLMNMKIFVDTDADVRLSRRIQRDTVERGRNIQNVLEQYTKF 206

Query: 240 RKPDFDAYIDPQKQFADAVI 255
            KP FD YI P  ++AD +I
Sbjct: 207 VKPSFDEYIQPSMKYADIII 208

[Close]

BLAST of CmaCh16G012010 vs. TAIR 10
Match: AT1G55810.1 (uridine kinase-like 3 )

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-13
Identity = 56/217 (25.81%), Postives = 100/217 (46.08%), Query Frame = 0


Query: 44  GKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTL 103
           G AS  P  +       Q  VIG+A  +  GK+T    +                     
Sbjct: 26  GLASNRPEQMAEEEEHGQPFVIGVAGGAASGKTTVCDMIMQ------------------Q 85

Query: 104 ISDTTTVICLDD--YHSLDRTGRKEKGVTALD-PRANDFDLMYEQVKALKDGIAVEKPIY 163
           + D   V+   D  YH+++            D P A D + +   ++ L+ G AV+ P Y
Sbjct: 86  LHDQRAVVVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNY 145

Query: 164 NHVT--GLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDM 223
           +  +    + PP  + P  ++++EG+   +D RVRDL++  I++D   +V+ A +I+RD 
Sbjct: 146 DFKSYKNNVFPPRRVNPSDVIILEGILIFHDPRVRDLMNMKIFVDADADVRLARRIKRDT 205

Query: 224 AERGHSLESIKASI-EARKPDFDAYIDPQKQFADAVI 255
            E+G  + ++     +  KP F+ +I P K++AD +I
Sbjct: 206 VEKGRDIATVLDQYSKFVKPAFEDFILPTKKYADIII 224

[Close]

BLAST of CmaCh16G012010 vs. TAIR 10
Match: AT1G55810.3 (uridine kinase-like 3 )

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-13
Identity = 56/217 (25.81%), Postives = 100/217 (46.08%), Query Frame = 0


Query: 44  GKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTL 103
           G AS  P  +       Q  VIG+A  +  GK+T    +                     
Sbjct: 26  GLASNRPEQMAEEEEHGQPFVIGVAGGAASGKTTVCDMIMQ------------------Q 85

Query: 104 ISDTTTVICLDD--YHSLDRTGRKEKGVTALD-PRANDFDLMYEQVKALKDGIAVEKPIY 163
           + D   V+   D  YH+++            D P A D + +   ++ L+ G AV+ P Y
Sbjct: 86  LHDQRAVVVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNY 145

Query: 164 NHVT--GLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDM 223
           +  +    + PP  + P  ++++EG+   +D RVRDL++  I++D   +V+ A +I+RD 
Sbjct: 146 DFKSYKNNVFPPRRVNPSDVIILEGILIFHDPRVRDLMNMKIFVDADADVRLARRIKRDT 205

Query: 224 AERGHSLESIKASI-EARKPDFDAYIDPQKQFADAVI 255
            E+G  + ++     +  KP F+ +I P K++AD +I
Sbjct: 206 VEKGRDIATVLDQYSKFVKPAFEDFILPTKKYADIII 224

[Close]

BLAST of CmaCh16G012010 vs. TAIR 10
Match: AT1G55810.2 (uridine kinase-like 3 )

HSP 1 Score: 75.1 bits (183), Expect = 1.5e-13
Identity = 56/217 (25.81%), Postives = 100/217 (46.08%), Query Frame = 0


Query: 44  GKASTTPWLITCSAGDQQTVVIGLAADSGCGKSTFMRRLTSVFGGAAKPPRGGNPDSNTL 103
           G AS  P  +       Q  VIG+A  +  GK+T    +                     
Sbjct: 26  GLASNRPEQMAEEEEHGQPFVIGVAGGAASGKTTVCDMIMQ------------------Q 85

Query: 104 ISDTTTVICLDD--YHSLDRTGRKEKGVTALD-PRANDFDLMYEQVKALKDGIAVEKPIY 163
           + D   V+   D  YH+++            D P A D + +   ++ L+ G AV+ P Y
Sbjct: 86  LHDQRAVVVNQDSFYHNVNEVELVRVHDYNFDHPDAFDTEQLLSSMEKLRKGQAVDIPNY 145

Query: 164 NHVT--GLLDPPELIKPPKILVIEGLHPMYDSRVRDLLDFSIYLDISNEVKFAWKIQRDM 223
           +  +    + PP  + P  ++++EG+   +D RVRDL++  I++D   +V+ A +I+RD 
Sbjct: 146 DFKSYKNNVFPPRRVNPSDVIILEGILIFHDPRVRDLMNMKIFVDADADVRLARRIKRDT 205

Query: 224 AERGHSLESIKASI-EARKPDFDAYIDPQKQFADAVI 255
            E+G  + ++     +  KP F+ +I P K++AD +I
Sbjct: 206 VEKGRDIATVLDQYSKFVKPAFEDFILPTKKYADIII 224

The following BLAST results are available for this feature:

BLAST of CmaCh16G012010 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P27774	1.4e-189	81.77	Phosphoribulokinase, chloroplastic OS=Mesembryanthemum crystallinum OX=3544 PE=2...	[more]
P26302	2.5e-183	80.16	Phosphoribulokinase, chloroplastic OS=Triticum aestivum OX=4565 PE=2 SV=1	[more]
P25697	2.1e-182	77.28	Phosphoribulokinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g32060 ...	[more]
P09559	5.3e-178	75.88	Phosphoribulokinase, chloroplastic OS=Spinacia oleracea OX=3562 PE=1 SV=1	[more]
P19824	2.8e-155	73.60	Phosphoribulokinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 GN=PRKA ...	[more]

BLAST of CmaCh16G012010 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32060.1	1.5e-183	77.28	phosphoribulokinase	[more]
AT3G27440.1	7.7e-15	26.50	uridine kinase-like 5	[more]
AT1G55810.1	1.5e-13	25.81	uridine kinase-like 3	[more]
AT1G55810.3	1.5e-13	25.81	uridine kinase-like 3	[more]
AT1G55810.2	1.5e-13	25.81	uridine kinase-like 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006082	Phosphoribulokinase	PRINTS	PR00478	PHRIBLKINASE	coord: 69..86 score: 60.0 coord: 178..196 score: 54.39 coord: 213..230 score: 66.67 coord: 231..254 score: 56.67
IPR006082	Phosphoribulokinase	PROSITE	PS00567	PHOSPHORIBULOKINASE	coord: 213..226
IPR006083	Phosphoribulokinase/uridine kinase	PFAM	PF00485	PRK	coord: 64..262 e-value: 1.0E-77 score: 260.3
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 59..285 e-value: 9.2E-65 score: 220.6
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 61..333
None	No IPR available	PANTHER	PTHR10285	URIDINE KINASE	coord: 6..408
None	No IPR available	PANTHER	PTHR10285:SF150	PHOSPHORIBULOKINASE	coord: 6..408
None	No IPR available	CDD	cd02026	PRK	coord: 64..353 e-value: 0.0 score: 534.995

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G012010.1	CmaCh16G012010.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0019253	reductive pentose-phosphate cycle
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0008974	phosphoribulokinase activity
molecular_function	GO:0016301	kinase activity