CmaCh16G011900 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G011900
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
LocationCma_Chr16: 9089026 .. 9091924 (+)
RNA-Seq ExpressionCmaCh16G011900
SyntenyCmaCh16G011900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGTCAACAAACTTCCACGTCACTGGCCGTGGAGTAGGCCGGCGCCGCCGTCGGCGGAGATTGCAGCTTGCCGGAGAAGAATCGTGGAGAGGGGCTACCCGAAAGAGAGGCCGTTATCCGATTTTCGGAAAGTTAGGACCGAGGTCTATAAAAGGGTAAAAAGGACTTTTCACTCCTAAAAACTGGTGAGATTTCCCTAGGATTTCATTTTGGACGGACGTCTTCATTTGTTCTTTGTTCATTGAGGCTCCAGAGCTCGGTATTGCAATGGCGAGATCCACAAGCAAGGACGCTCAAGCTCTTTTCCAGTCGCTTCGCTCTGCTTATGCTGCCACTCCAACAACTCTCAAGGTATTATATCTTTTCACTCCTGCTCTAAATTTTGCTGATGGAATTTTGACCTAGATCGTCTTCCTCGCAGATCATTGATCTCTATGTGATTTATGCTGTGTTCACGGCTCTGATTCAGGTTTGTGCCGTTTTGATCCTTTTGTGGACGCCATTTCCTTGATTATTGATAGTTTTGTGTTGTTCCGGAACGGATTTGTATCGAATTTTCCTTTTCTGCGTCCGAATTCGTCAATGTGTCACGCTCTTTTGATTACTGTCTTTGAACTGTATTCGATTGTAGAAAACTGCATTGCAATGGAAAAATGAATGCAAGTTTGACGTTGCTTCTGTTTTCTTTACTTTTTATAAAATACGATCTAGCCTGTTATTTTACCAGACTCCATCCTGGTTTTTATATCTCAGCATAAGTTCTTGTTCCTCTGCCAATCATTTTTTTTATGAGCAACCTTCTTTGAAAATGGAAGAATACAATAGAACGGAATCCTCTTGCTTGCTTAAACTGGTGCAGAAATTTTCCAAGTACCTCAAACAGTACAAGCTAATAATTACAAAAATCTGAGTCGCCTCAAGAACACATAAAAAGTAATGTTGCCTAAGCATCTTGAAAATTCATCTCGTACCATGAACCCAACAAAGGGATCGGCCTTTCATGCCGAGTCCATTTGTGGAGGAATGTTGCGATCTTATCTTGATACCCTTTACATAATGGCACGTATTATGTGCTTCATCTGTTCAATTCAACGTTTGGCCTCTTTCTTTTGATAAAGCTTTAATTGAATATTCATTATAAGAGATTAACTAACTCACCGAGTTCTGAAAGTTTCCTGATTTCCTTGGTAAGTTATAACTCTCTTTTTTCGTTTAATTGAATACTTGAGCTTGTTTCATTTACGTTGAATAAATTTTATCAACAAAAAGTGCTGTTGGAGTGGAAGGTTTCATTAAGTGTAGGCTGTGCTTGATAAACTAATGATACTCAACTATTTGCATTTGACCTTTGTTTTATAATCGTGGGTCTGTATCGTTGGCATTCACATTTAAACTTGGTTGAGTTTATGAGTCATCGTTTGGATTTTCTACGCATTTTATATTTTGAACTGTGATGCCTAACTAAATAAATAGAACATATGTATTAGATGAATGACAATGTAATGAAAAATTAGATGATATTTTTTTAGGTGATTCTTTAGCTATAGGCTTTTTAGGTTTATCTATGCAACATAAATTTATAAGGTCAGAAATATCTAACAGCACGCACCTTTTGCGCTGTGGAGATGCCTTCATCTTGCACAACGCCGAGTCGCACTTTTAAAATGTTTCCGAGTTCTTAAATTTGTTTTGAATTACTTTTAACTGATAAGTTCTTGTAAACAGGTAGCATACATGGCGATTGTTGGATCGTTCCCATTCAACTCCTTCCTCTCAGGCGTTCTTTCTTGTATCGGGACAGCTGTCCTTGCTGGTAAGCAGGATGTATATCTTGGTTATCAAATCACAAAATAATGTGAGGAGCAATTCCTATTCTGATCGAATTCTTCATTTTCAGTTTGTCTCCGGATTCAAGTTAATAAAGAAAACAAGGAGTTCAAGGTAATTTCTTTCATGTTTTTCTACTTAATTTGACCTCACTTATATTTTTCATTTGCATATTAGGAAAGAGTATGCGTAAAATCGTTATTTTCTTTTCATTAACAACGAATATTCCAAATTGTAACAGTTTAAGCCCACCGCTAGTTGATATTGTCCTCTTTGGACTTTCTCTTTCGAGCTTCTCTTCAAGATTTTTAAAACGCGTATGATAGGGAGAGGTTTTCATACCCTTATAAAGAATTATTCGTTCTCCTCTCCGGCTGATGCGGGATCTGCAATAAAATTTTGAACAATTTGTTCAAATACTTATCATTCCTCACATTCCTCACATGATTTGAAATGAAAGGCATGAACCTCCATTTATTATGGGAAAAATAAATAATAGACGAACTTCCTATCATGAAATCCAAAAATTGGTTACAACACGTTTCGAAATGCTTAACGAGTCCTCTTCAAATTTTCAGGATTTGGCCCCGGAGCGAGCTTTTGCTGATTTCGTGCTGTGCAACCTGGTTCTCCATTTGGTGATCATCTGCTTCCTCGGATAAAACACCTCATTTTGGGTTCTACTTCTTTTCTTGTTTTCTTTGGAGAATTAATTTAAACCCAGGAAGTAGGCTTATGCTAGAAATTTTCTACCAAACATAGCATACATATTAGAAAGAGAGAGTTATTCCTGCAACTGAATTAATGTCATTCTGAGTTATTTTCTACAGTGATGTTTTTCCAGACAATCAAATGCGTCAACACTTTTCCGGCCATTATGTTGCAAAGGTAAGAACTATACTTGCACACTCTCTGTCACTTAAACAATGAAGATAACAGAAAACAAGTTCTTAACTCTTGCAACTGCTAGAAGCTGAAATTGTGAATTAAGCTCTTGCACAACTGCATGTTCTATCTCAGTATGTTTCTTTTTTTTCTTTTTCAGAATTTTCTATATATCTTTTTCAC

mRNA sequence

ATGAAAGTCAACAAACTTCCACGTCACTGGCCGTGGAGTAGGCCGGCGCCGCCGTCGGCGGAGATTGCAGCTTGCCGGAGAAGAATCGTGGAGAGGGGCTACCCGAAAGAGAGGCCGTTATCCGATTTTCGGAAAGTTAGGACCGAGGCTCCAGAGCTCGGTATTGCAATGGCGAGATCCACAAGCAAGGACGCTCAAGCTCTTTTCCAGTCGCTTCGCTCTGCTTATGCTGCCACTCCAACAACTCTCAAGATCATTGATCTCTATGTGATTTATGCTGTGTTCACGGCTCTGATTCAGGTAGCATACATGGCGATTGTTGGATCGTTCCCATTCAACTCCTTCCTCTCAGGCGTTCTTTCTTGTATCGGGACAGCTGTCCTTGCTGTTTGTCTCCGGATTCAAGTTAATAAAGAAAACAAGGAGTTCAAGGATTTGGCCCCGGAGCGAGCTTTTGCTGATTTCGTGCTGTGCAACCTGGTTCTCCATTTGGTGATCATCTGCTTCCTCGGATAAAACACCTCATTTTGGTGATGTTTTTCCAGACAATCAAATGCGTCAACACTTTTCCGGCCATTATGTTGCAAAGGTAAGAACTATACTTGCACACTCTCTGTCACTTAAACAATGAAGATAACAGAAAACAAGTTCTTAACTCTTGCAACTGCTAGAAGCTGAAATTGTGAATTAAGCTCTTGCACAACTGCATGTTCTATCTCAGTATGTTTCTTTTTTTTCTTTTTCAGAATTTTCTATATATCTTTTTCAC

Coding sequence (CDS)

ATGAAAGTCAACAAACTTCCACGTCACTGGCCGTGGAGTAGGCCGGCGCCGCCGTCGGCGGAGATTGCAGCTTGCCGGAGAAGAATCGTGGAGAGGGGCTACCCGAAAGAGAGGCCGTTATCCGATTTTCGGAAAGTTAGGACCGAGGCTCCAGAGCTCGGTATTGCAATGGCGAGATCCACAAGCAAGGACGCTCAAGCTCTTTTCCAGTCGCTTCGCTCTGCTTATGCTGCCACTCCAACAACTCTCAAGATCATTGATCTCTATGTGATTTATGCTGTGTTCACGGCTCTGATTCAGGTAGCATACATGGCGATTGTTGGATCGTTCCCATTCAACTCCTTCCTCTCAGGCGTTCTTTCTTGTATCGGGACAGCTGTCCTTGCTGTTTGTCTCCGGATTCAAGTTAATAAAGAAAACAAGGAGTTCAAGGATTTGGCCCCGGAGCGAGCTTTTGCTGATTTCGTGCTGTGCAACCTGGTTCTCCATTTGGTGATCATCTGCTTCCTCGGATAA

Protein sequence

MKVNKLPRHWPWSRPAPPSAEIAACRRRIVERGYPKERPLSDFRKVRTEAPELGIAMARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG
Homology
[Close]
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match: Q9M3T9 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=Betula pendula OX=3505 GN=DAD1 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-50
Identity = 105/115 (91.30%), Postives = 111/115 (96.52%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           MARSTSKDAQAL QSLRSAYAATP+ LKIIDLYV++AVFTALIQV YMAIVGSFPFN+FL
Sbjct: 1   MARSTSKDAQALIQSLRSAYAATPSNLKIIDLYVVFAVFTALIQVVYMAIVGSFPFNAFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SG+LSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVI+ FLG
Sbjct: 61  SGLLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIMNFLG 115

[Close]
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match: Q39080 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 6.7e-50
Identity = 104/115 (90.43%), Postives = 110/115 (95.65%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           M +STSKDAQ LF+SLRSAY+ATPT LKIIDLYV++AVFTALIQV YMA+VGSFPFNSFL
Sbjct: 1   MVKSTSKDAQDLFRSLRSAYSATPTNLKIIDLYVVFAVFTALIQVVYMALVGSFPFNSFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVII FLG
Sbjct: 61  SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG 115

[Close]
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match: Q9ZWQ7 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=Citrus unshiu OX=55188 GN=DAD1 PE=3 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 8.8e-50
Identity = 105/115 (91.30%), Postives = 109/115 (94.78%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           MARST KDAQALF SLRSAYAATPTTLKIIDLYV +AVFTALIQV YMAIVGSFPFNSFL
Sbjct: 1   MARSTGKDAQALFHSLRSAYAATPTTLKIIDLYVGFAVFTALIQVVYMAIVGSFPFNSFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SGVLSC+GTAVLAVCLRIQVNK+NKEFKDL PERAFADFVLCNLVLHLVI+ FLG
Sbjct: 61  SGVLSCVGTAVLAVCLRIQVNKDNKEFKDLPPERAFADFVLCNLVLHLVIMNFLG 115

[Close]
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match: O22622 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2 OS=Arabidopsis thaliana OX=3702 GN=DAD2 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 4.4e-49
Identity = 104/115 (90.43%), Postives = 107/115 (93.04%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           M +STSKDAQ LF SL SAY ATPT LKIIDLYV +AVFTALIQVAYMA+VGSFPFNSFL
Sbjct: 1   MVKSTSKDAQDLFHSLHSAYTATPTNLKIIDLYVCFAVFTALIQVAYMALVGSFPFNSFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVII FLG
Sbjct: 61  SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG 115

[Close]
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match: Q9SMC4 (Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=Solanum lycopersicum OX=4081 GN=DAD1 PE=3 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 1.4e-47
Identity = 101/112 (90.18%), Postives = 105/112 (93.75%), Query Frame = 0 

Query: 60  STSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFLSGV 119
           S +KDAQALF SLRSAYAATPT LKIIDLYVI+A+ TALIQV YMAIVGSFPFNSFLSGV
Sbjct: 5   SATKDAQALFHSLRSAYAATPTNLKIIDLYVIFAISTALIQVVYMAIVGSFPFNSFLSGV 64

Query: 120 LSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           LSCIGTAVLAVCLRIQVNKENKEFKDL PERAFADFVLCNLVLHLVI+ FLG
Sbjct: 65  LSCIGTAVLAVCLRIQVNKENKEFKDLPPERAFADFVLCNLVLHLVIMNFLG 116

[Close]
BLAST of CmaCh16G011900 vs. TAIR 10
Match: AT1G32210.1 (Defender against death (DAD family) protein )

HSP 1 Score: 198.4 bits (503), Expect = 4.8e-51
Identity = 104/115 (90.43%), Postives = 110/115 (95.65%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           M +STSKDAQ LF+SLRSAY+ATPT LKIIDLYV++AVFTALIQV YMA+VGSFPFNSFL
Sbjct: 1   MVKSTSKDAQDLFRSLRSAYSATPTNLKIIDLYVVFAVFTALIQVVYMALVGSFPFNSFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVII FLG
Sbjct: 61  SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG 115

[Close]
BLAST of CmaCh16G011900 vs. TAIR 10
Match: AT2G35520.1 (Defender against death (DAD family) protein )

HSP 1 Score: 195.7 bits (496), Expect = 3.1e-50
Identity = 104/115 (90.43%), Postives = 107/115 (93.04%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALIQVAYMAIVGSFPFNSFL 116
           M +STSKDAQ LF SL SAY ATPT LKIIDLYV +AVFTALIQVAYMA+VGSFPFNSFL
Sbjct: 1   MVKSTSKDAQDLFHSLHSAYTATPTNLKIIDLYVCFAVFTALIQVAYMALVGSFPFNSFL 60

Query: 117 SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVII FLG
Sbjct: 61  SGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG 115

[Close]
BLAST of CmaCh16G011900 vs. TAIR 10
Match: AT2G35520.2 (Defender against death (DAD family) protein )

HSP 1 Score: 191.0 bits (484), Expect = 7.6e-49
Identity = 104/116 (89.66%), Postives = 107/116 (92.24%), Query Frame = 0 

Query: 57  MARSTSKDAQALFQSLRSAYAATPTTLKIIDLYVIYAVFTALI-QVAYMAIVGSFPFNSF 116
           M +STSKDAQ LF SL SAY ATPT LKIIDLYV +AVFTALI QVAYMA+VGSFPFNSF
Sbjct: 1   MVKSTSKDAQDLFHSLHSAYTATPTNLKIIDLYVCFAVFTALIQQVAYMALVGSFPFNSF 60

Query: 117 LSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIICFLG 172
           LSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVII FLG
Sbjct: 61  LSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG 116

The following BLAST results are available for this feature:
BLAST of CmaCh16G011900 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9M3T93.0e-5091.30Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=B... [more]
Q390806.7e-5090.43Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=A... [more]
Q9ZWQ78.8e-5091.30Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=C... [more]
O226224.4e-4990.43Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2 OS=A... [more]
Q9SMC41.4e-4790.18Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 OS=S... [more]
BLAST of CmaCh16G011900 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G32210.14.8e-5190.43Defender against death (DAD family) protein [more]
AT2G35520.13.1e-5090.43Defender against death (DAD family) protein [more]
AT2G35520.27.6e-4989.66Defender against death (DAD family) protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003038DAD/Ost2PFAMPF02109DADcoord: 68..171
e-value: 2.2E-36
score: 124.5
IPR003038DAD/Ost2PANTHERPTHR10705DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1coord: 58..171
NoneNo IPR availablePANTHERPTHR10705:SF1DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1coord: 58..171

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G011900.1CmaCh16G011900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0008250 oligosaccharyltransferase complex
molecular_function GO:0016740 transferase activity