CmaCh16G011230 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G011230
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description2-oxoglutarate-Fe(II) type oxidoreductase-like
LocationCma_Chr16: 8630870 .. 8635716 (+)
RNA-Seq ExpressionCmaCh16G011230
SyntenyCmaCh16G011230
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGGTTCTGGTTCTACTGCTCTTCGACCGAAAATGGCGTCTGTTCTGCAACTCCCAATCATCGACCTCTCCTCTCAAGACCGCACCTCTGTTGCTTTCTCAATCCATCAGGTTCTTCGTATGATTTCCAAATCAACTCTCTCCATCGTTTTCCCTAACAGATTCTGATATTAATCCGTTTTCTTGGATTGGAAATTACGCATAGGCATGCTTGGATTACGGATTCTTTTACTTGATCAACCATGGCGTTGAAGACGAATTGCTCGAGAGAGTGTTTGACGAAAGTAGGAAGTTCTTTTCTCTTCCTATTGAGGAGAAGATGAAACTTGCTCGGAAGGAACATAGGGGATACACCGCTCTTTATGCCGAGAAGCTCGATCCCACCACCTCGACTGATATAGGTTTTTGTTCTATTTTGATTTGAAATTTATGAATCTCGTCTGTTTATCTTGTGAAATTTGGGAGAAATTTGAACTGTGAGAGCTGTGTAAATGGTAGTTCTGCCTGGCTGTTGCTCCGAGCTTCTGAATGTTGTATGAAATGGTCTAAGAAAACCAATTAGTAAGTAAACTAACTATAAGATTAAGAGATTAGAATAGCTATAACTTCTAGATTGAGACACTTTTATTAATGTTGTTTATAGATATGATGGCTGTGAAAATGCAACAATCCGGATTATATGATCTCTTTTAAAATTATGATTGTGTAATATTAGATGAGGTGTGTGCAAGCTGACCCAGACACTCGTTGATATTAGAAAAAAAGATTGATTGCATATGGTTTTCCAGAAAGGTGAAAAGATGGTATATCCACGGACCATACTAGTCCACATTTTAGTGTTTTGTTCGACTGTAAATGATCATTTCCTAGTAGGGAGAGTGGATTGACTCGATAAAAGAACAAAAATGTGAGATCTCACATTGGTTTGAGGGGGGACGAATCATTCCTTATAAGAGTGTGAAAACCTCTATCTAGTAGACGCGTTTTAAAACCTTGAGTGAAAGCCCGAAAGAGAAAGTCCAAAGAGGACAATATCTACTAGTGGTGGGGTTGGGCCGTTACAAATGGTATCAGATGCTTCAACGAAAACGCTGGGCTTACAAGGGGGTGGATTGTGAGATCCCACATTGGTGCGAGGGGGGAAACATTCCTTATAAGGGTGTGAAAAAACCTCCCTAGTAGATGCGTTTGAAACACCTTGAGGGGAAGCTCGAAAGGGAAAGAAATCTTTAGGGAAAACTCGAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGCGTTTTAAAAACCTTGAGAGGAAGCCCGAAAGGAAAAGTCCAACGAGGACAATATCTGCTAGCGGTGGGCTTGGGTCGTTACAAAAAGAATCATAAGAAGAGTAAGAATCTTAAAAAATATCCAAACTCATATCATCATTGAACTGTTTTGTTAGATTGTAAATGGTGCAGATTGGAAACTAATGCACAATCTCTACCGTTATTGAAGATTTGGTGTTGGAGAAAGTTGTTTATCTTTGTATCTATGATTTTTCTGTGAATAGGGGATGCAAAAGAGAGCTATTATATTGGCCCTTTGGCAGGCAACGAAACTGAAAATGACTTAAATCAGTGGCCTTCAGGAGGTGACCAATTACCCTACTTATGGTAGAAATTTGAAATCATTGATTATTCATGTTTATGAACTATTACTCTTTCTGGCAAGGAAATGACAGAACTATTGCCTTCTTGGAGAGCTACAATGGAATCCTTCTATAAGCAAGCACTGTAAGTTCAAATGCCTTCAGGATTAATTTCAATACATCCTGGTACTTATCAGTGCAATAAAATTGGTACTATGTTGTTTTAAATTCATGTTTTGTTCATTGTACTCGAAAGTATTCTTATCTCGTTTACGCTGGATGGCTAAATGAATTTGGACTGGCTGTAATGTCTTGCTTTCTGTTTGGTTGGTACAGGCGTGTTGGAAGACGAATAATATCTTTACTTGCATTGGCTCTGAATCTGGATGAGAATTTCTTTGAGAAAATTGGTGCCTTCAGTAAACCATCAGATTTTCTTCGGCTTCTACACTATCCAGGTTCAGTGGACATACGTAACTTTTAATTACTGAAAACTACAGGGGAGAAAAGAAGCATATGTTCGGTTTTTTCTTCCTGTCCCCACCCCCAACATTTATGCTGAATTTTGTAAAAGATAAAATTTAAAGATGATGAAGATGAAGATGAAATTTAAAGAAGTGTGATGGAACCCCCCAAAATGTGGGAATGGGAATGGTCTATGGAAATGATTTTCAATCTTAGCGAGGATCCCACAAAATTGCAAACCATCTTATAGTTTTTTGATAAAATTTGGAAACAATATGAATAGCAAAGAGGTAGAATGATTCTCCTTCCCTCATTCTCATTTCCTTCTAAGTTAATGATGTACTTGGTCAGTTAACGACCCAAACCCACTGCTAACAGATATTATCTTCTTTTGGCTTTCCCTTTTGAGCTTCCTCTCAAGGTTTTAAAACACGTCTGTTAGGGGAGGTTTCCACACCCTTGTAAAGAATGCTTCATTCTCCTCCCTAATCGATGTGGGATCTCACAATCCACCCTTCTTTGGGGCCCAACGTCCTCGCTGGCACTCATTCCCTTCTTCAATTGATATGAGACCCCCCAATCCAACCCTCTTCGGGACCCAGCGTCCTTGTTGGCACATCGTCCCGTGTTTGGCTTTGATACCATTTGTAACTGCTCATGCTCGCTGCTGCTAGCAGATATTGTCCTCTTTGGGCTTCCCCTCAAGGTTTTTAAAACACGTCTACTAGGGAAAGGTTTCCACATCCTTATAAAGAATATTTCGTTCTCCTCCCCAACCAATGTGAGATCTCACACATTTCGTCTTCAAGTCGATGGATGCAATCTTGTTGATAATCTGCTCATTCTCTTGAACTCAGGTGACTTAAGATCTCTTGATGAAGAAATATTAGGTGCTTCTGCACATTCAGATTGGGGGATGATCACTCTCCTTGTAACCAATGGCATTCCCGGACTTCAGGTATTTCTCCTTAATCAATTGAGATCATACTTTATAGAGAAAATTGTGTGTATTATGCGGTGAAGTTTTGTGTTAAGTTGCCATGGTAACAGATATGCAAGGAAAAGTCTGATCACCCACAAGTTTGGGAAGATGTTGCTCATATAGATAAGTGAGTTCAAAACTCAGAATTGAAAACAATTGAACATTATAAAATGATGCTCCAAAGACACCACAAGGGAAGCCTCATGACTGTCTGGAATTCAATGGATGCAATTCTCTGATATTGCAGGGCATTCATTGTTAATGTTGGGGACTTGATGGAAAGGTGGACGAACTGTCTGTTCCGGTAAGGATTGAGGTTGTACGTTATGATAAATTCTATCATGTGAAAGTAAAAAACTAGATTCCATTGTTGGTTATTTGCTTGTTTTTGTGTTATTTACGATAGGTCGACTCTACATCGAGTGATTCCAGTTGGAGAAGAACGCTACTCGGTAAGGTCTAGCTATTTACTTGTTTGCTTTCTGCCAATAAGCCCATCCAAGAAACTATGATTTGAACCCTATTTTGAGGAAGCTTTTTTTAACGGCCCAATCCTACTGCTAGCAAATATTGTCCTCTTTGAACTTTCCTTCAAGGTTTTTAAAACGTGTTTGCTAGAGAGAGGTTTCCATATCTTTGTAAAGAATATTTCGTTCTCTTTCCCAACCAATGTGAGATAAAAGTGATTTTTTAAACTTCAAATATCTCTCCATTTTGCAGGTTGCTTTCTTCTTACATCCGAGTGACGAATGTGTCGTGGAATGTCTTCATAGTTGTTGTAGCGAGTCATCTCCCCCGAGGTATTGACGTTCCTTACTTTTATAATATTATCCCCGGAATCTCTCCATTCCCTTAGGAATTTGATCCAATAATAATCTTATAGGACAGTTCTACAAAACTAATCATATGAAATTGTGTTATAAGAAGAATTACAATGCTCTTTCTCCAATTTTTTGGCTACTCCGAGTCGTTTTACCACTTATGAAGTCACAATTCAACTGAATATAACCTTAAAAACTAAAATTGTGAGATCCCACATCGATTGTAAAGAGAAACGAGTATTAGCAAAGACGCTGGGCCTCGAGGGGGTGGATTGTGAGATCCCACCTCAATTGGAGAGGGGAATGAAATATTCTTTATAAGTGTGTAGAAACCTCTATTTAGCAGACGCGTTTTAAAAACTTTGAGGGGAAAGCCTGAAAGGAAAGCCCAAAGAAGACGATATCTGTTAGCGATGGGTTAAGGTTCTTACAAAAGATCAATCTTGAATGAGTTCTAATGTCTATGCTGGCCTTTAGTTTGAACACAATCCTTGGACTATCTCCATGCTTCTCCGACTTCATGCATCAATTGCTAGGAATGAGTTTTATCGGTCGTTTTACGAGTATAAATAACCTGAAGAACATTGATCAAAATACATTTTCATGATTTCATGGATTACATGAAAATTTTCCTCATCATGATCTCTAAAACAACTACAAATTTAAATGCTTAGATGCTTCAAGATTATTGAATGTGCATGCAGATATCCTCCCATTAGCAGTCTAGACTACTTGAAAGAACGTGTGAGGTTGGCTTATAAAGACGGATTTGCTTAGAAAAGGTAAGATTCACTCCTCTCACTCCTATGTTTCTAGATTAAATTTAGATCAAGAAGCTTGATAAGAGTTCATTTACATCATCACTAATGGATAAAGACTTGATGTGTATGCATATTATGATAACCTTCTCAAAATTTATCATGTTACTGCCTTGACAATGACGTTAATGATAGAGAT

mRNA sequence

GTGGTTCTGGTTCTACTGCTCTTCGACCGAAAATGGCGTCTGTTCTGCAACTCCCAATCATCGACCTCTCCTCTCAAGACCGCACCTCTGTTGCTTTCTCAATCCATCAGGCATGCTTGGATTACGGATTCTTTTACTTGATCAACCATGGCGTTGAAGACGAATTGCTCGAGAGAGTGTTTGACGAAAGTAGGAAGTTCTTTTCTCTTCCTATTGAGGAGAAGATGAAACTTGCTCGGAAGGAACATAGGGGATACACCGCTCTTTATGCCGAGAAGCTCGATCCCACCACCTCGACTGATATAGGGGATGCAAAAGAGAGCTATTATATTGGCCCTTTGGCAGGCAACGAAACTGAAAATGACTTAAATCAGTGGCCTTCAGGAGAACTATTGCCTTCTTGGAGAGCTACAATGGAATCCTTCTATAAGCAAGCACTGCGTGTTGGAAGACGAATAATATCTTTACTTGCATTGGCTCTGAATCTGGATGAGAATTTCTTTGAGAAAATTGGTGCCTTCAGTAAACCATCAGATTTTCTTCGGCTTCTACACTATCCAGGTGACTTAAGATCTCTTGATGAAGAAATATTAGGTGCTTCTGCACATTCAGATTGGGGGATGATCACTCTCCTTGTAACCAATGGCATTCCCGGACTTCAGATATGCAAGGAAAAGTCTGATCACCCACAAGTTTGGGAAGATGTTGCTCATATAGATAAGGCATTCATTGTTAATGTTGGGGACTTGATGGAAAGGTGGACGAACTGTCTGTTCCGGTCGACTCTACATCGAGTGATTCCAGTTGGAGAAGAACGCTACTCGGTTGCTTTCTTCTTACATCCGAGTGACGAATGTGTCGTGGAATGTCTTCATAGTTGTTGTAGCGAGTCATCTCCCCCGAGATATCCTCCCATTAGCAGTCTAGACTACTTGAAAGAACGTGTGAGGTTGGCTTATAAAGACGGATTTGCTTAGAAAAGGTAAGATTCACTCCTCTCACTCCTATGTTTCTAGATTAAATTTAGATCAAGAAGCTTGATAAGAGTTCATTTACATCATCACTAATGGATAAAGACTTGATGTGTATGCATATTATGATAACCTTCTCAAAATTTATCATGTTACTGCCTTGACAATGACGTTAATGATAGAGAT

Coding sequence (CDS)

ATGGCGTCTGTTCTGCAACTCCCAATCATCGACCTCTCCTCTCAAGACCGCACCTCTGTTGCTTTCTCAATCCATCAGGCATGCTTGGATTACGGATTCTTTTACTTGATCAACCATGGCGTTGAAGACGAATTGCTCGAGAGAGTGTTTGACGAAAGTAGGAAGTTCTTTTCTCTTCCTATTGAGGAGAAGATGAAACTTGCTCGGAAGGAACATAGGGGATACACCGCTCTTTATGCCGAGAAGCTCGATCCCACCACCTCGACTGATATAGGGGATGCAAAAGAGAGCTATTATATTGGCCCTTTGGCAGGCAACGAAACTGAAAATGACTTAAATCAGTGGCCTTCAGGAGAACTATTGCCTTCTTGGAGAGCTACAATGGAATCCTTCTATAAGCAAGCACTGCGTGTTGGAAGACGAATAATATCTTTACTTGCATTGGCTCTGAATCTGGATGAGAATTTCTTTGAGAAAATTGGTGCCTTCAGTAAACCATCAGATTTTCTTCGGCTTCTACACTATCCAGGTGACTTAAGATCTCTTGATGAAGAAATATTAGGTGCTTCTGCACATTCAGATTGGGGGATGATCACTCTCCTTGTAACCAATGGCATTCCCGGACTTCAGATATGCAAGGAAAAGTCTGATCACCCACAAGTTTGGGAAGATGTTGCTCATATAGATAAGGCATTCATTGTTAATGTTGGGGACTTGATGGAAAGGTGGACGAACTGTCTGTTCCGGTCGACTCTACATCGAGTGATTCCAGTTGGAGAAGAACGCTACTCGGTTGCTTTCTTCTTACATCCGAGTGACGAATGTGTCGTGGAATGTCTTCATAGTTGTTGTAGCGAGTCATCTCCCCCGAGATATCCTCCCATTAGCAGTCTAGACTACTTGAAAGAACGTGTGAGGTTGGCTTATAAAGACGGATTTGCTTAG

Protein sequence

MASVLQLPIIDLSSQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPIEEKMKLARKEHRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETENDLNQWPSGELLPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYPGDLRSLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNVGDLMERWTNCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPISSLDYLKERVRLAYKDGFA
Homology
BLAST of CmaCh16G011230 vs. ExPASy Swiss-Prot
Match: C8VK14 (2-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=hxnY PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 2.7e-44
Identity = 109/328 (33.23%), Postives = 176/328 (53.66%), Query Frame = 0

Query: 8   PIIDLS------SQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPI 67
           PI+D S         +  +   + ++C   GFF +  H +  EL  RV D +++FF+LP+
Sbjct: 7   PILDFSPFYGTDGAAKAKLVQQVRESCEYNGFFQITGHRIPRELQVRVMDAAKRFFALPL 66

Query: 68  EEKM---KLARKEHRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAG--------NETEN 127
           EEKM   K     +RGY  L ++ L+  T+ ++   KE  YIG   G            +
Sbjct: 67  EEKMAIDKNLNSFNRGYELLRSQMLEVGTAPEL---KEGLYIGEEIGADHPYYINGRLNS 126

Query: 128 DLNQWPSGELLP---SWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPS 187
             NQWP+   +P    +R T   +Y     + + ++++LAL L+++E+FF+ +      +
Sbjct: 127 GPNQWPA--TVPDAQEFRETSMEYYHAVYELAKDVLAVLALTLDVEESFFDPLTEGGVAT 186

Query: 188 DFLRLLHYPGDLRSLDEEI-LGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVA 247
             +R+LHYP   +  DE++  G  AH+D+G ITLL+ + + GLQ+    S     W DV 
Sbjct: 187 --MRMLHYPSQPKDEDEKLNRGIGAHTDFGCITLLLQDEVDGLQVLDAPSGQ---WLDVQ 246

Query: 248 HIDKAFIVNVGDLMERWTNCLFRSTLHRVI-PVGEERYSVAFFLHPSDECVVECLHSCCS 307
            +  A++VN+GDLM R  N  ++S +HRVI   G ERYS+ FF   + + V +CL +CC 
Sbjct: 247 PVLGAYVVNLGDLMMRMANDRYKSNIHRVINKSGRERYSIPFFFSGNPDHVCKCLPNCCK 306

Query: 308 ESSPPRYPPISSLDYLKERVRLAYKDGF 314
               P+YPPI+    +++ VR AYK  +
Sbjct: 307 AGEQPKYPPIT----VEDMVRGAYKQSY 320

BLAST of CmaCh16G011230 vs. ExPASy Swiss-Prot
Match: Q4W946 (2-oxoglutarate-Fe(II) type oxidoreductase OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=encD PE=1 SV=2)

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-40
Identity = 106/320 (33.12%), Postives = 164/320 (51.25%), Query Frame = 0

Query: 8   PIIDLS------SQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPI 67
           PI+D S      SQ +  +   + + CL+ GFF +  H V  EL  R FD +++FF LP+
Sbjct: 8   PILDFSVFYGHDSQAKAQLVQRVRECCLNNGFFQITGHKVSPELQRRTFDCAKRFFDLPL 67

Query: 68  EEKMKLARKE---HRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNE----TENDL-- 127
            EK K+ R     +RGY A  +    P ++    D KE  ++GP    +     +  L  
Sbjct: 68  IEKKKIERSPDAFNRGYEAFQSHMSQPGSAP---DRKEGLFLGPDLAEDHPYCVQKKLNC 127

Query: 128 --NQWPSG-ELLPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKI--GAFSKPS 187
             N+WP G + L  ++     +Y    ++ + ++++LAL ++ +E FF+ +  GA +   
Sbjct: 128 GPNRWPQGLDDLEEFKLVSMEYYAALFQLAKDVVAVLALTMDYEETFFDPLTEGAIAT-- 187

Query: 188 DFLRLLHYPGDLRSLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAH 247
             LR LHYP       E  LG  AH D+  ITLL+ +G  GLQ+  E +     W DV  
Sbjct: 188 --LRYLHYPPQPVGDAEAGLGTGAHRDYSCITLLLQDGTGGLQVLDEPTGQ---WLDVKP 247

Query: 248 IDKAFIVNVGDLMERWTNCLFRSTLHRVI-PVGEERYSVAFFLHPSDECVVECLHSCCSE 307
           +  A+IVN+ ++  R TN  ++S LHRV+   G ERYS+ FF   + + V ECL     E
Sbjct: 248 VPGAYIVNLANVFARMTNGHYKSALHRVVNKSGMERYSIPFFFTGNPDYVCECLSRFRKE 307

BLAST of CmaCh16G011230 vs. ExPASy Swiss-Prot
Match: A0A166YZY4 (2-oxoglutarate-Fe(II) type oxidoreductase ppzD OS=Metarhizium rileyi (strain RCEF 4871) OX=1081105 GN=ppzD PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-35
Identity = 102/298 (34.23%), Postives = 152/298 (51.01%), Query Frame = 0

Query: 32  GFFYLINHGVEDELLERVFDESRKFFSLPIEEKMKLAR----KEHRGYTALYAEKLD--- 91
           GF  + NHG+  E +E+VF  ++ FFSLP   K K A       HRGY+ +  EKL    
Sbjct: 40  GFVKIRNHGISGEEIEKVFVMNKLFFSLPQAAKAKAAHPPEANPHRGYSYVGQEKLSRVK 99

Query: 92  -----PTTSTDIGDAKESYYIGPLAGNETENDLNQWPSGELLPSWRATMESFYKQALRVG 151
                  +  D+ D KESY  GP          N+WP  + +P +R  ME FY++  +V 
Sbjct: 100 DYEKGKRSIVDVYDIKESYDQGPAVDKLYP---NRWPDKQDIPGFRVVMEKFYERCHQVH 159

Query: 152 RRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYPG-DLRSLDEEILGASAHSDWGMI 211
           + ++  +A   +L  +F   +    + +  LRL HYPG    SL +     S H+D+G +
Sbjct: 160 QDVLRAIATGFDLSPSFLTDL--CCENTSELRLNHYPGVHPSSLRKGAKRISEHTDFGTV 219

Query: 212 TLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKA-FIVNVGDLMERWTNCLFRSTLHRVI- 271
           TLL  + + GL+I  E  + P  +  V+   K+  IVNVGD ++RWTN    ST HRV+ 
Sbjct: 220 TLLFQDSVGGLEI--EDQNSPGTYFPVSSERKSDMIVNVGDCIQRWTNDKILSTSHRVVL 279

Query: 272 -----PVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPISSLDYLKERVRLAY 310
                 + ++RYSVA+F  PS   +V  L     E   P+Y  IS+  Y +E++ L Y
Sbjct: 280 PEDRDALIKDRYSVAYFGKPSRSQLVSPLREFVKEGEKPKYSAISAWQYNQEKLVLTY 330

BLAST of CmaCh16G011230 vs. ExPASy Swiss-Prot
Match: K0E3U5 (2-oxoglutarate-dependent dioxygenase ecdK OS=Aspergillus rugulosus OX=41736 GN=ecdK PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 8.6e-35
Identity = 104/316 (32.91%), Postives = 165/316 (52.22%), Query Frame = 0

Query: 26  QACLD----YGFFYLINHGVEDELLERVFDESRKFFSLPIEEKMKLAR----KEHRGYTA 85
           QA LD     GF  LINHG   E +  +F+ +++FF LPI+ K  +      K  RG+++
Sbjct: 24  QALLDGFTTTGFVKLINHGFSKEEMADIFNWNQRFFDLPIDRKAAIRNDEGPKPQRGWSS 83

Query: 86  LYAEK---LDP--------TTSTDIGDAKESYYIGPLAGNETENDLNQWPSGELLPSWRA 145
           L AEK   L+P         ++ D  DAKE + IGP    E E   N+WP  + LP ++ 
Sbjct: 84  LGAEKTGFLNPGGKLSLARASNNDRQDAKEHFDIGPA---EDEEFQNKWPEEQTLPGFQE 143

Query: 146 TMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYPG-DLRSLDE- 205
           TM S++ ++  +   ++  LALA+++ ++ F  +G     +  LRL HYP   +++++E 
Sbjct: 144 TMNSYFDRSQAITLELLEALALAMDVPKDTF--VGLCHGHASELRLNHYPSIPVKTIEEG 203

Query: 206 EILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKA-FIVNVGDLMERW 265
           +      H+D+G+ITLL  + I GL+I  +  +HP  +  V   D   F+VN+GD++ERW
Sbjct: 204 KTNRIWPHTDFGIITLLAQDDIGGLEI--QDRNHPSDFLPVNREDSTEFVVNIGDILERW 263

Query: 266 TNCLFRSTLHRVIPVGE----------ERYSVAFFLHPSDECVVECLHSCCSESSPPRYP 310
           TN   R+ LH+V                R SVAFFL P  +  V  +      +  PRY 
Sbjct: 264 TNGRLRAGLHQVTTPRSMQQKGNGTLPTRRSVAFFLKPHRQMSVASISHFVPGNQSPRYE 323

BLAST of CmaCh16G011230 vs. ExPASy Swiss-Prot
Match: Q76NT9 (1-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum OX=44689 GN=aco PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.5e-34
Identity = 95/310 (30.65%), Postives = 162/310 (52.26%), Query Frame = 0

Query: 6   QLPIIDLSS-----QDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLP 65
           +LPIID+SS      D+  VA  I++AC +YGFFY+ NHGV+ EL+E + +  +KFFSLP
Sbjct: 7   KLPIIDISSFQNNENDKNQVAKEINKACKEYGFFYIKNHGVDQELIENLQNVIKKFFSLP 66

Query: 66  IEEKMK----LARKEHRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGN-ETENDLNQW 125
           +E KMK    L  +E  G+  +  E     T   +   +  YY   + G+  T +++  +
Sbjct: 67  LEIKMKWKMGLTNREWLGFFKVGQE----ITYGQVDWKEGCYYSSEMDGDINTIHNVPLY 126

Query: 126 PSGEL-----LPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFL 185
           P+ E      +  +++T+ ++ ++   + ++II  ++L+LNL +++F K   +  P   +
Sbjct: 127 PTAEQEEQYEIQGFKSTIHTYIEKLTHLSQQIIEAISLSLNLPQDYFFKNYTYD-PFILM 186

Query: 186 RLLHYPG--------------------------DLRSLDEEILGASAHSDWGMITLLVTN 245
            LLHYP                           + +  + E  G   H+DWG++T+L  +
Sbjct: 187 GLLHYPSFHHQEQEEEQEDDESNNGGKKSPNPDESKKPEVEKFGTGQHTDWGLLTVLYQD 246

Query: 246 GIPGLQICKEKSDHPQVWEDVAHIDKAFIVNVGDLMERWTNCLFRSTLHRV--IPVGEER 273
            + GLQ+   KS + + + D   I   FI N+GD++++ T   + S LHRV     G +R
Sbjct: 247 DVGGLQV---KSKNSEEYIDAPPIPGTFICNIGDMLDKMTGGYYLSNLHRVKYNKSGRDR 306

BLAST of CmaCh16G011230 vs. TAIR 10
Match: AT4G16770.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 371.3 bits (952), Expect = 7.6e-103
Identity = 175/304 (57.57%), Postives = 227/304 (74.67%), Query Frame = 0

Query: 1   MASVLQLPIIDLSSQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLP 60
           M + L+LPIIDLSS ++ S +  I QACLD+GFFYL NHGV +EL+E V  ES+K FSLP
Sbjct: 11  MGTALKLPIIDLSSPEKLSTSRLIRQACLDHGFFYLTNHGVSEELMEGVLIESKKLFSLP 70

Query: 61  IEEKMKLARKEHRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETENDLNQWPSGEL 120
           ++EKM +AR   RGY+ LY EKL+ ++ST IGD+KE +  G   G   +   N+WP  EL
Sbjct: 71  LDEKMVMARHGFRGYSPLYDEKLE-SSSTSIGDSKEMFTFGSSEGVLGQLYPNKWPLEEL 130

Query: 121 LPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYPGDLR 180
           LP WR TME +YK  + VG+++  L+ALALNL+EN+FE++GAF+  +  +RLL Y G+  
Sbjct: 131 LPLWRPTMECYYKNVMDVGKKLFGLVALALNLEENYFEQVGAFNDQAAVVRLLRYSGESN 190

Query: 181 SLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNVGDLM 240
           S  EE  GASAHSD+GMITLL T+G+ GLQ+C++K   P+VWEDVA I   F+VN+GDLM
Sbjct: 191 SSGEETCGASAHSDFGMITLLATDGVAGLQVCRDKDKEPKVWEDVAGIKGTFVVNIGDLM 250

Query: 241 ERWTNCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPISSLDY 300
           ERWTN LFRSTLHRV+ VG+ER+SVA F+ P   CVVECL SCCSE+SPP++PP+ + DY
Sbjct: 251 ERWTNGLFRSTLHRVVSVGKERFSVAVFVDPDPNCVVECLESCCSETSPPKFPPVRARDY 310

Query: 301 LKER 305
             ER
Sbjct: 311 FHER 313

BLAST of CmaCh16G011230 vs. TAIR 10
Match: AT4G16765.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 332.8 bits (852), Expect = 3.0e-91
Identity = 152/245 (62.04%), Postives = 194/245 (79.18%), Query Frame = 0

Query: 65  MKLARKEHRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETENDLNQWPSGELLPSW 124
           M L R++  GYT LYAEKLDP+ S+ IGD+KES+Y G L G   +   NQWPS  +LPSW
Sbjct: 1   MALLRRDLLGYTPLYAEKLDPSLSS-IGDSKESFYFGSLEGVLAQRYPNQWPSEGILPSW 60

Query: 125 RATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYPGDLRSLDE 184
           R TME++YK  L VGR+++ L+ALAL+LDE+FFEK+GA + P+  +RLL YPG++ S D 
Sbjct: 61  RQTMETYYKNVLSVGRKLLGLIALALDLDEDFFEKVGALNDPTAVVRLLRYPGEVISSDV 120

Query: 185 EILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNVGDLMERWT 244
           E  GASAHSD+GM+TLL+T+G+PGLQ+C++KS  P +WEDV  I  AFIVN+GD+MERWT
Sbjct: 121 ETYGASAHSDYGMVTLLLTDGVPGLQVCRDKSKQPHIWEDVPGIKGAFIVNIGDMMERWT 180

Query: 245 NCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPISSLDYLKER 304
           N LFRSTLHRV+PVG+ERYSV FFL P+ +C V+CL SCCSE+ PPR+PPI + DY+KER
Sbjct: 181 NGLFRSTLHRVMPVGKERYSVVFFLDPNPDCNVKCLESCCSETCPPRFPPILAGDYIKER 240

Query: 305 VRLAY 310
            RL Y
Sbjct: 241 FRLTY 244

BLAST of CmaCh16G011230 vs. TAIR 10
Match: AT1G35190.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-82
Identity = 151/308 (49.03%), Postives = 203/308 (65.91%), Query Frame = 0

Query: 4   VLQLPIIDLSSQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPIEE 63
           V  L  IDL++ D      S+ QACLD GFFY+INHG+ +E ++ VF++S+K F+LP+EE
Sbjct: 9   VSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEE 68

Query: 64  KMKLARKE-HRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETEN------DLNQWP 123
           KMK+ R E HRGYT +  E LDP    + GD KE YYIG     +  +        N WP
Sbjct: 69  KMKVLRNEKHRGYTPVLDELLDPKNQIN-GDHKEGYYIGIEVPKDDPHWDKPFYGPNPWP 128

Query: 124 SGELLPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYP 183
             ++LP WR TME ++++ALRV   I  LLALAL+LD  +F++     KP   +RLL Y 
Sbjct: 129 DADVLPGWRETMEKYHQEALRVSMAIARLLALALDLDVGYFDRTEMLGKPIATMRLLRYQ 188

Query: 184 GDLRSLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNV 243
           G +    + I    AHSD+GM+TLL T+G+ GLQICK+K+  PQ WE V  I  AFIVN+
Sbjct: 189 G-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPIKGAFIVNL 248

Query: 244 GDLMERWTNCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPIS 303
           GD++ERW+N  F+STLHRV+  G+ERYS+ FF+ P+ +C+VECL +C SES  P+YPPI 
Sbjct: 249 GDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESELPKYPPIK 308

Query: 304 SLDYLKER 305
              YL +R
Sbjct: 309 CSTYLTQR 314

BLAST of CmaCh16G011230 vs. TAIR 10
Match: AT3G46490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 278.9 bits (712), Expect = 5.1e-75
Identity = 139/313 (44.41%), Postives = 195/313 (62.30%), Query Frame = 0

Query: 4   VLQLPIIDLSSQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPIEE 63
           V  L  IDL + D    A  + QACLD GFFY+INHG+ +EL +  F+ S+KFF+LP+EE
Sbjct: 14  VSSLTCIDLDNSDLHQSAVLLKQACLDSGFFYVINHGISEELKDEAFEHSKKFFALPLEE 73

Query: 64  KMKLARKE-HRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETE------NDLNQWP 123
           KMK+ R E +RGY   +   LDP      GD KE + IG     +        +  N WP
Sbjct: 74  KMKVLRNEKYRGYAPFHDSLLDPENQVR-GDYKEGFTIGFEGSKDGPHWDKPFHSPNIWP 133

Query: 124 SGELLPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYP 183
           + ++LP WR TME +Y++ALRV + I  ++ALAL+LD ++F        P   + L HY 
Sbjct: 134 NPDVLPGWRETMEKYYQEALRVCKSIAKIMALALDLDVDYFNTPEMLGNPIADMVLFHYE 193

Query: 184 GDLRSLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNV 243
           G      + I    AHSD+GM++LL T+G+ GLQICK+K   PQ WE    I  A+IVN+
Sbjct: 194 GK-SDPSKGIYACGAHSDFGMMSLLATDGVMGLQICKDKDVKPQKWEYTPSIKGAYIVNL 253

Query: 244 GDLMERWTNCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPIS 303
           GDL+ERW+N  F+STLHRV+  G++RYS+ FFL PS +C++ECL +C SE++ P+YP I 
Sbjct: 254 GDLLERWSNGYFKSTLHRVLGNGQDRYSIPFFLKPSHDCIIECLPTCQSENNLPKYPAIK 313

Query: 304 SLDYLKERVRLAY 310
              Y+ +R +  +
Sbjct: 314 CSTYISQRYKAVH 324

BLAST of CmaCh16G011230 vs. TAIR 10
Match: AT1G35190.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.4e-72
Identity = 141/308 (45.78%), Postives = 190/308 (61.69%), Query Frame = 0

Query: 4   VLQLPIIDLSSQDRTSVAFSIHQACLDYGFFYLINHGVEDELLERVFDESRKFFSLPIEE 63
           V  L  IDL++ D      S+ QACLD GFFY+INHG+ +E ++ VF++S+K F+LP+EE
Sbjct: 9   VSSLNCIDLANDDLNHSVVSLKQACLDCGFFYVINHGISEEFMDDVFEQSKKLFALPLEE 68

Query: 64  KMKLARKE-HRGYTALYAEKLDPTTSTDIGDAKESYYIGPLAGNETEN------DLNQWP 123
           KMK+ R E HRGYT +  E LDP    + GD KE YYIG     +  +        N WP
Sbjct: 69  KMKVLRNEKHRGYTPVLDELLDPKNQIN-GDHKEGYYIGIEVPKDDPHWDKPFYGPNPWP 128

Query: 124 SGELLPSWRATMESFYKQALRVGRRIISLLALALNLDENFFEKIGAFSKPSDFLRLLHYP 183
             ++LP WR TME ++++AL                     E +G   KP   +RLL Y 
Sbjct: 129 DADVLPGWRETMEKYHQEALT--------------------EMLG---KPIATMRLLRYQ 188

Query: 184 GDLRSLDEEILGASAHSDWGMITLLVTNGIPGLQICKEKSDHPQVWEDVAHIDKAFIVNV 243
           G +    + I    AHSD+GM+TLL T+G+ GLQICK+K+  PQ WE V  I  AFIVN+
Sbjct: 189 G-ISDPSKGIYACGAHSDFGMMTLLATDGVMGLQICKDKNAMPQKWEYVPPIKGAFIVNL 248

Query: 244 GDLMERWTNCLFRSTLHRVIPVGEERYSVAFFLHPSDECVVECLHSCCSESSPPRYPPIS 303
           GD++ERW+N  F+STLHRV+  G+ERYS+ FF+ P+ +C+VECL +C SES  P+YPPI 
Sbjct: 249 GDMLERWSNGFFKSTLHRVLGNGQERYSIPFFVEPNHDCLVECLPTCKSESELPKYPPIK 291

Query: 304 SLDYLKER 305
              YL +R
Sbjct: 309 CSTYLTQR 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
C8VK142.7e-4433.232-oxoglutarate-Fe(II) type oxidoreductase hxnY OS=Emericella nidulans (strain FG... [more]
Q4W9461.1e-4033.132-oxoglutarate-Fe(II) type oxidoreductase OS=Neosartorya fumigata (strain ATCC M... [more]
A0A166YZY41.3e-3534.232-oxoglutarate-Fe(II) type oxidoreductase ppzD OS=Metarhizium rileyi (strain RCE... [more]
K0E3U58.6e-3532.912-oxoglutarate-dependent dioxygenase ecdK OS=Aspergillus rugulosus OX=41736 GN=e... [more]
Q76NT91.5e-3430.651-aminocyclopropane-1-carboxylate oxidase OS=Dictyostelium discoideum OX=44689 G... [more]
Match NameE-valueIdentityDescription
AT4G16770.17.6e-10357.572-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G16765.13.0e-9162.042-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G35190.11.9e-8249.032-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G46490.15.1e-7544.412-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G35190.21.4e-7245.782-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00682IPNSYNTHASEcoord: 232..258
score: 38.05
coord: 24..41
score: 58.59
coord: 108..124
score: 25.67
coord: 259..277
score: 36.36
coord: 141..157
score: 44.39
NoneNo IPR availablePANTHERPTHR10209OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEINcoord: 5..303
NoneNo IPR availablePANTHERPTHR10209:SF5902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEINcoord: 5..303
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 4..310
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 2..312
e-value: 6.4E-102
score: 343.5
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 170..271
e-value: 1.2E-17
score: 64.2
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 7..117
e-value: 2.2E-31
score: 109.0
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 166..271
score: 12.2256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G011230.1CmaCh16G011230.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity