CmaCh16G010940 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G010940
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionBeta-amylase
LocationCma_Chr16: 8427258 .. 8430320 (+)
RNA-Seq ExpressionCmaCh16G010940
SyntenyCmaCh16G010940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTTAACACTACGATCTTCGACTTCTTTCATCAACCCGAAAGACAACAAGATCATAAAGGCTCATCTTCATGAGGATCTCTATGGCGTAATCTCCTTCAAGCAAGCAAAATCATCAACCCGTCTTCGAAAAAGAAGCTCCCTACAAGAGATTAAGATGGAAAGGAGTTACAAATTGGAGAAACTTCATTCGTTATCTATGAATCATCACATGAATGACTTGAGAGTTCCTGTGTTTGTTATGTTGCCGCTTGACACGGTTACGCTTGGAGGGAATCTGAACAAGCCTCGAGCGATGAACGCAAGTTTAATGGCTTTGAAGAGTGCGGGTGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGACCTATGAAATACAATTGGGAAGGCTATGCTGAGCTTGTGCAGATGCTTCAAAAGCATGGCTTGAAGCTCCAAGTAGTCATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGGTATGAATCCGAGAACATTTTCTTAACCATTTTCGACTTTTCGATACTCGGTTAACCTTCTCTCTATGATCTTTCAGCATTCCTTTACCGCCATGGGTGCTCGAAGAAATAAGCAAGAACCCAGATCTCGTTTATACCGACAGATCAGGCAGGAGGAATCCGGAATACATCTCTTTGGGATGTGATTCCTTACCTGTGCTTAGAGGAAGAACACCAATCCAAGTCTATGCTGATTACATGAGGAGCTTTCGGGATCGATTTAGAGACTACTTGGGAGAAGTTATCATGGTATGCATCGACAATTTGTTCAATTCTCTTAATCCTTCTTCTTCTTAACCGTCTCCGAACACCTTTAATCCTCCCACAAGATGATAGCTTAGCTTAGTTATATGTGTAGTATAGCTTGGAATGAACATGACTCGATCACTGTGAGATCTCACATCGGTTGGGGAGGAGAACAAAACACCCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACGTGTTTTAAAAACCTTGAAGGGAAGCCCGAAAGGGAAAGTCCAAAAAGGACAATATCTGCTAGTGGTGGGCTTTGTCTGTTTCAAATGGTATCAGAGCCAGACATCGGGCGATGTGCCAGCGAGGAGGCTGTTCCATGAACGGGGTAGACACGAGGCAGTGTGCGAGCGAGGACGCTAGTTCTCAAAGGGGGTCGATTTGGTGCGGATCCCACATTGATTGAAAAAAGGAACGAATGTCAGCGAGGACACTAGGCCCTGAAGGGGGGTGGATTGTGAGATCTCACATCGGTTGGGGGGAGAACGAAACACCCTTTACAAGGGTTTGGAAACCTCTTCCTAGTAGACGCGTTTAAAAACCTAGAGAGGAAGCTTGAAAGGGAAAGCTCAAAAGAACAATATCAGATAGCGATGGGCTTGGGCCGTTACAATCACGTAGATAGAATTCAAGTAAATAGCTTCCAGAGTTGGAAATTCAATGTTCATTTGCAATTGCAGGAAGTTCAAGTCGGGGCTGGTCCATGTGGGGAACTCAGATATCCGTCTTACCCACAGAGCAATGGAACTTGGAGATTTCCAGGAATCGGGGAATTCCAATGTTACGACAAGGTACTCGACTTGATATACTTCTTTTTACGTATTGATCTAACCGTAACAGGATCCTTCTACAGCAATATCAAACTAGGGGCTGTTTAGAGAAAATGGTTTTTGAGAACGATTTTTGATATGTCTTCTGTGGTGAACAGTACATGAGAGCTTCCCTTGAGGCAGCAGCAGAGAGCATTGGGAAAAGAGACTGGGGAAGCAATGGAGGGCCACATGATTCTGGTCAATACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAAGGAAGGAACATGGAACACAGAATACGGCAAGTTCTTCCTCGAATGGTACTCGGGCAAGCTATTACAGCACGGGGATAGAATCTTAGCAGCTTCGAAGGGAATTTTCCATGGAACGGGTGCAAAACTATCAGCAAAAGTAGCTGGAATTCACTGGCATTACGGTACTCGATCCCATGCCGCCGAGTTAACCGCAGGGTACTACAACACTCGACACCGAAACGGTTACTCGCCTATTGCGAAGATGCTAGCTAAACATGGGGTTGTGTTTAACTTCACTTGCATGGAAATGAAGGACGGACAGCAGCCCGGCTATGCGAACTGCTCACCGGAAGGTTTGGTTAGACAAGTGAAGATGGCTACAAGGGATGCTAAGGCTGAACTTGCAGGTGAAAATGCATTGGAAAGGTATGATGGAGGAGCTTATGAGCAAATTTTAGCCACGAGTAGGTCAGAATCTGGAAATGGCTTGACTGCATTCACTTACTTGAGAATGAACAAGAACCTTTTCGAACCCAACAATTGGAGAAACTTGGTCGAGTTTGTAAAGACCATGTCGGAAGGCGGTCGGAACCGGAAACTTCCGGAATCGGACTGCAGTGGCAGTGATCTTCACGTTGGATTCATTAAGGAGAAAAAGATCAAGGAAGTTGCTGTGGTGTAGAAATGAAACCAGCACATACTTATGAGTCTTATAGTTAAAAGATAAGGAGAAAAAGAAAAAGGGTATAGGAAAAGTCTTAAATCTACAGCCAAAACTCTCATAATCCTCATACTTCTATGTGTAAAGATGTAGTTTTTTATACTTTCAGAACAGTAATTATTGAGTCCTTCAGTAAAAAAGTTGGACCAAGAAGGGATCTAGCATAGATATAGGTAAGAACATGAGTGAAACAGCCATCTACGATCCAGAATGGATACACATAATGGTGTATAATATGGTACTCAGATCAGATTTATTATACTTTCAGATATCACGACGTGCTTAAAAGACGAATTTTCGGGAGTTTCAGGTCAAAACTTTTCATATTTCATCTAGAAGAATGTAAAGTAAGCAAGTGTAGATATCAGAAGATCAAACCTTTCCTTGTCTTCCAATCATGATATGCTTTTGATCAAGAAATCTTGGAGAAAACGGGACGCCATGAGACTGTTTCTGATTACTATTGGAGCTTGTTTTCTCTTCCGA

mRNA sequence

ATGACTTTAACACTACGATCTTCGACTTCTTTCATCAACCCGAAAGACAACAAGATCATAAAGGCTCATCTTCATGAGGATCTCTATGGCGTAATCTCCTTCAAGCAAGCAAAATCATCAACCCGTCTTCGAAAAAGAAGCTCCCTACAAGAGATTAAGATGGAAAGGAGTTACAAATTGGAGAAACTTCATTCGTTATCTATGAATCATCACATGAATGACTTGAGAGTTCCTGTGTTTGTTATGTTGCCGCTTGACACGGTTACGCTTGGAGGGAATCTGAACAAGCCTCGAGCGATGAACGCAAGTTTAATGGCTTTGAAGAGTGCGGGTGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGACCTATGAAATACAATTGGGAAGGCTATGCTGAGCTTGTGCAGATGCTTCAAAAGCATGGCTTGAAGCTCCAAGTAGTCATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGCATTCCTTTACCGCCATGGGTGCTCGAAGAAATAAGCAAGAACCCAGATCTCGTTTATACCGACAGATCAGGCAGGAGGAATCCGGAATACATCTCTTTGGGATGTGATTCCTTACCTGTGCTTAGAGGAAGAACACCAATCCAAGTCTATGCTGATTACATGAGGAGCTTTCGGGATCGATTTAGAGACTACTTGGGAGAAGTTATCATGGAAGTTCAAGTCGGGGCTGGTCCATGTGGGGAACTCAGATATCCGTCTTACCCACAGAGCAATGGAACTTGGAGATTTCCAGGAATCGGGGAATTCCAATGTTACGACAAGTACATGAGAGCTTCCCTTGAGGCAGCAGCAGAGAGCATTGGGAAAAGAGACTGGGGAAGCAATGGAGGGCCACATGATTCTGGTCAATACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAAGGAAGGAACATGGAACACAGAATACGGCAAGTTCTTCCTCGAATGGTACTCGGGCAAGCTATTACAGCACGGGGATAGAATCTTAGCAGCTTCGAAGGGAATTTTCCATGGAACGGGTGCAAAACTATCAGCAAAAGTAGCTGGAATTCACTGGCATTACGGTACTCGATCCCATGCCGCCGAGTTAACCGCAGGGTACTACAACACTCGACACCGAAACGGTTACTCGCCTATTGCGAAGATGCTAGCTAAACATGGGGTTGTGTTTAACTTCACTTGCATGGAAATGAAGGACGGACAGCAGCCCGGCTATGCGAACTGCTCACCGGAAGGTTTGGTTAGACAAGTGAAGATGGCTACAAGGGATGCTAAGGCTGAACTTGCAGGTGAAAATGCATTGGAAAGGTATGATGGAGGAGCTTATGAGCAAATTTTAGCCACGAGTAGGTCAGAATCTGGAAATGGCTTGACTGCATTCACTTACTTGAGAATGAACAAGAACCTTTTCGAACCCAACAATTGGAGAAACTTGGTCGAGTTTGTAAAGACCATGTCGGAAGGCGGTCGGAACCGGAAACTTCCGGAATCGGACTGCAGTGGCAGTGATCTTCACGTTGGATTCATTAAGGAGAAAAAGATCAAGGAAGTTGCTGTGGTGTAGAAATGAAACCAGCACATACTTATGAGTCTTATAGTTAAAAGATAAGGAGAAAAAGAAAAAGGGTATAGGAAAAGTCTTAAATCTACAGCCAAAACTCTCATAATCCTCATACTTCTATGTGTAAAGATGTAGTTTTTTATACTTTCAGAACAGTAATTATTGAGTCCTTCAGTAAAAAAGTTGGACCAAGAAGGGATCTAGCATAGATATAGGTAAGAACATGAGTGAAACAGCCATCTACGATCCAGAATGGATACACATAATGGTGTATAATATGGTACTCAGATCAGATTTATTATACTTTCAGATATCACGACGTGCTTAAAAGACGAATTTTCGGGAGTTTCAGGTCAAAACTTTTCATATTTCATCTAGAAGAATGTAAAGTAAGCAAGTGTAGATATCAGAAGATCAAACCTTTCCTTGTCTTCCAATCATGATATGCTTTTGATCAAGAAATCTTGGAGAAAACGGGACGCCATGAGACTGTTTCTGATTACTATTGGAGCTTGTTTTCTCTTCCGA

Coding sequence (CDS)

ATGACTTTAACACTACGATCTTCGACTTCTTTCATCAACCCGAAAGACAACAAGATCATAAAGGCTCATCTTCATGAGGATCTCTATGGCGTAATCTCCTTCAAGCAAGCAAAATCATCAACCCGTCTTCGAAAAAGAAGCTCCCTACAAGAGATTAAGATGGAAAGGAGTTACAAATTGGAGAAACTTCATTCGTTATCTATGAATCATCACATGAATGACTTGAGAGTTCCTGTGTTTGTTATGTTGCCGCTTGACACGGTTACGCTTGGAGGGAATCTGAACAAGCCTCGAGCGATGAACGCAAGTTTAATGGCTTTGAAGAGTGCGGGTGTGGAAGGAGTTATGGTGGATGCTTGGTGGGGATTGGTGGAGAAAGATGGACCTATGAAATACAATTGGGAAGGCTATGCTGAGCTTGTGCAGATGCTTCAAAAGCATGGCTTGAAGCTCCAAGTAGTCATGTCTTTTCATCAGTGTGGAGGGAATGTTGGAGATTCTTGCAGCATTCCTTTACCGCCATGGGTGCTCGAAGAAATAAGCAAGAACCCAGATCTCGTTTATACCGACAGATCAGGCAGGAGGAATCCGGAATACATCTCTTTGGGATGTGATTCCTTACCTGTGCTTAGAGGAAGAACACCAATCCAAGTCTATGCTGATTACATGAGGAGCTTTCGGGATCGATTTAGAGACTACTTGGGAGAAGTTATCATGGAAGTTCAAGTCGGGGCTGGTCCATGTGGGGAACTCAGATATCCGTCTTACCCACAGAGCAATGGAACTTGGAGATTTCCAGGAATCGGGGAATTCCAATGTTACGACAAGTACATGAGAGCTTCCCTTGAGGCAGCAGCAGAGAGCATTGGGAAAAGAGACTGGGGAAGCAATGGAGGGCCACATGATTCTGGTCAATACAACCAGTTCCCTGAAGATACAGGCTTCTTCAAAAAGGAAGGAACATGGAACACAGAATACGGCAAGTTCTTCCTCGAATGGTACTCGGGCAAGCTATTACAGCACGGGGATAGAATCTTAGCAGCTTCGAAGGGAATTTTCCATGGAACGGGTGCAAAACTATCAGCAAAAGTAGCTGGAATTCACTGGCATTACGGTACTCGATCCCATGCCGCCGAGTTAACCGCAGGGTACTACAACACTCGACACCGAAACGGTTACTCGCCTATTGCGAAGATGCTAGCTAAACATGGGGTTGTGTTTAACTTCACTTGCATGGAAATGAAGGACGGACAGCAGCCCGGCTATGCGAACTGCTCACCGGAAGGTTTGGTTAGACAAGTGAAGATGGCTACAAGGGATGCTAAGGCTGAACTTGCAGGTGAAAATGCATTGGAAAGGTATGATGGAGGAGCTTATGAGCAAATTTTAGCCACGAGTAGGTCAGAATCTGGAAATGGCTTGACTGCATTCACTTACTTGAGAATGAACAAGAACCTTTTCGAACCCAACAATTGGAGAAACTTGGTCGAGTTTGTAAAGACCATGTCGGAAGGCGGTCGGAACCGGAAACTTCCGGAATCGGACTGCAGTGGCAGTGATCTTCACGTTGGATTCATTAAGGAGAAAAAGATCAAGGAAGTTGCTGTGGTGTAG

Protein sequence

MTLTLRSSTSFINPKDNKIIKAHLHEDLYGVISFKQAKSSTRLRKRSSLQEIKMERSYKLEKLHSLSMNHHMNDLRVPVFVMLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEGYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRNPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSYPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRSHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVKMATRDAKAELAGENALERYDGGAYEQILATSRSESGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKTMSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAVV
Homology
BLAST of CmaCh16G010940 vs. ExPASy Swiss-Prot
Match: O23553 (Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3)

HSP 1 Score: 812.4 bits (2097), Expect = 3.1e-234
Identity = 393/551 (71.32%), Postives = 457/551 (82.94%), Query Frame = 0

Query: 1   MTLTLRSSTSFINPKDNKIIKAHLHEDLYGVISFKQAKSST-RLRKRSSLQEIKM----- 60
           M LTL SS+S I  KD K   +   E     ++F + K  T + + ++S++E+K      
Sbjct: 1   MELTLNSSSSLIKRKDAK--SSRNQESSSNNMTFAKMKPPTYQFQAKNSVKEMKFTHEKT 60

Query: 61  -----ERSYKLEKLHSLSMNHHMNDLRVPVFVMLPLDTVTLGGNLNKPRAMNASLMALKS 120
                E   K EKLH LS  H  ND  VPVFVMLPLDTVT+ G+LNKPRAMNASLMALK 
Sbjct: 61  FTPEGETLEKWEKLHVLSYPHSKNDASVPVFVMLPLDTVTMSGHLNKPRAMNASLMALKG 120

Query: 121 AGVEGVMVDAWWGLVEKDGPMKYNWEGYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCS 180
           AGVEGVMVDAWWGLVEKDGPM YNWEGYAEL+QM+QKHGLKLQVVMSFHQCGGNVGDSCS
Sbjct: 121 AGVEGVMVDAWWGLVEKDGPMNYNWEGYAELIQMVQKHGLKLQVVMSFHQCGGNVGDSCS 180

Query: 181 IPLPPWVLEEISKNPDLVYTDRSGRRNPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDR 240
           IPLPPWVLEEISKNPDLVYTD+SGRRNPEYISLGCDS+PVLRGRTPIQVY+D+MRSFR+R
Sbjct: 181 IPLPPWVLEEISKNPDLVYTDKSGRRNPEYISLGCDSVPVLRGRTPIQVYSDFMRSFRER 240

Query: 241 FRDYLGEVIMEVQVGAGPCGELRYPSYPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESI 300
           F  Y+G VI E+QVG GPCGELRYPSYP+SNGTWRFPGIGEFQCYDKYM++SL+A AESI
Sbjct: 241 FEGYIGGVIAEIQVGMGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMKSSLQAYAESI 300

Query: 301 GKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAAS 360
           GK +WG++ GPHD+G+Y   PEDT FF+++GTWN+EYGKFF+EWYSGKLL+HGD++L+++
Sbjct: 301 GKTNWGTS-GPHDAGEYKNLPEDTEFFRRDGTWNSEYGKFFMEWYSGKLLEHGDQLLSSA 360

Query: 361 KGIFHGTGAKLSAKVAGIHWHYGTRSHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNF 420
           KGIF G+GAKLS KVAGIHWHY TRSHAAELTAGYYNTR+ +GY PIAKM  KHGVV NF
Sbjct: 361 KGIFQGSGAKLSGKVAGIHWHYNTRSHAAELTAGYYNTRNHDGYLPIAKMFNKHGVVLNF 420

Query: 421 TCMEMKDGQQPGYANCSPEGLVRQVKMATRDAKAELAGENALERYDGGAYEQILATSRSE 480
           TCMEMKDG+QP +ANCSPEGLV+QV+ ATR A  ELAGENALERYD  A+ Q++AT+RS+
Sbjct: 421 TCMEMKDGEQPEHANCSPEGLVKQVQNATRQAGTELAGENALERYDSSAFGQVVATNRSD 480

Query: 481 SGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKTMSEGGRNRKLPESDCSGSDLHVGFIKEK 538
           SGNGLTAFTYLRMNK LFE  NW+ LVEFVK M EGG  R+L + D +GSDL+VGF+K K
Sbjct: 481 SGNGLTAFTYLRMNKRLFEGQNWQQLVEFVKNMKEGGHGRRLSKEDTTGSDLYVGFVKGK 540

BLAST of CmaCh16G010940 vs. ExPASy Swiss-Prot
Match: Q10RZ1 (Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 PE=1 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 6.4e-171
Identity = 278/433 (64.20%), Postives = 348/433 (80.37%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGN-LNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWE 136
           VPV+VM+PLDTV   GN LN+ +A+ ASL ALKSAG EG+MVD WWG+ E +GP +YN+ 
Sbjct: 93  VPVYVMMPLDTVRKDGNGLNRRKAVEASLKALKSAGAEGIMVDVWWGIAECEGPGRYNFT 152

Query: 137 GYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRR 196
           GY EL++M +K+GLK+Q VMSFHQCGGNVGDS +IPLP WVLEE+ K+ DL YTDRSGRR
Sbjct: 153 GYMELMEMAKKNGLKVQAVMSFHQCGGNVGDSVTIPLPKWVLEEMDKDQDLAYTDRSGRR 212

Query: 197 NPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPS 256
           N EY+SLG D++PVL+GRTP+Q Y D+MR+FRD F  ++G  I+E+QVG GP GELRYPS
Sbjct: 213 NYEYLSLGADAMPVLKGRTPVQCYGDFMRAFRDHFAAFMGNTIVEIQVGMGPAGELRYPS 272

Query: 257 YPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGF 316
           YP+SNGTWRFPGIGEFQCYD+YM +SL+AAAE++GK +WG N GP DSG YN +PED+ F
Sbjct: 273 YPESNGTWRFPGIGEFQCYDRYMLSSLKAAAEAVGKPEWG-NAGPGDSGGYNDWPEDSPF 332

Query: 317 FKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGT-GAKLSAKVAGIHWHYGTR 376
           F++EG WNT YG+FF+ WYS  LL+HG+RIL+A+ G++ GT G K+S KVAGIHWHYGTR
Sbjct: 333 FRREGGWNTPYGEFFMSWYSQMLLEHGERILSAASGVYTGTPGVKISVKVAGIHWHYGTR 392

Query: 377 SHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQV 436
           SHAAELTAGYYNTRH +GY PIA+MLA+HG V NFTC+EM++ +QP  A C PE LV+QV
Sbjct: 393 SHAAELTAGYYNTRHHDGYQPIARMLARHGAVLNFTCVEMRNHEQPQDAQCRPEELVQQV 452

Query: 437 KMATRDAKAELAGENALERYDGGAYEQILATSRSES-GNGLTAFTYLRMNKNLFEPNNWR 496
             A R++   LAGENAL RYD  A++QI+ T+  ++    + AFTYLRM  +LF+P+NWR
Sbjct: 453 AAAARESGVGLAGENALPRYDETAHDQIVTTAAEKAEEERMVAFTYLRMGPDLFQPDNWR 512

Query: 497 NLVEFVKTMSEGG 507
               FVK M+E G
Sbjct: 513 RFAAFVKRMTESG 524

BLAST of CmaCh16G010940 vs. ExPASy Swiss-Prot
Match: Q9LIR6 (Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.8e-170
Identity = 288/468 (61.54%), Postives = 354/468 (75.64%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEG 136
           VPVFVM+PLD+VT+G  +N+ +AM ASL ALKSAGVEG+M+D WWGLVEK+ P  YNW G
Sbjct: 106 VPVFVMMPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGG 165

Query: 137 YAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRN 196
           Y EL+++ +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+PDL YTD+ GRRN
Sbjct: 166 YNELLELAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRN 225

Query: 197 PEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSY 256
            EYISLG D+LPVL+GRTP+Q YAD+MR+FRD F+  LGE I+E+QVG GP GELRYPSY
Sbjct: 226 HEYISLGADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSY 285

Query: 257 PQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFF 316
           P+  GTW+FPGIG FQCYDKY  +SL+AAAE+ GK +WGS  GP D+G YN +PEDT FF
Sbjct: 286 PEQEGTWKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGST-GPTDAGHYNNWPEDTQFF 345

Query: 317 KKE-GTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRS 376
           KKE G WN+EYG FFL WYS  LL HG+RIL+++K IF   G K+S K+AGIHWHYGTRS
Sbjct: 346 KKEGGGWNSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRS 405

Query: 377 HAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVK 436
           HA ELTAGYYNTR R+GY PIA+MLA+H  +FNFTC+EM+D +QP  A C+PE LV QV 
Sbjct: 406 HAPELTAGYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVA 465

Query: 437 MATRDAKAELAGENALERYDGGAYEQIL---ATSRSESGNG----LTAFTYLRMNKNLFE 496
           +AT  A+  LAGENAL RYD  A+EQIL   A +  ++  G    + AFTYLRMN  LF+
Sbjct: 466 LATLAAEVPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQ 525

Query: 497 PNNWRNLVEFVKTMSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAV 537
            +NW   V FVK M EG  + +  E +      H   + +  ++E AV
Sbjct: 526 ADNWGKFVAFVKKMGEGRDSHRCRE-EVEREAEHFVHVTQPLVQEAAV 571

BLAST of CmaCh16G010940 vs. ExPASy Swiss-Prot
Match: Q9AV88 (Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 PE=1 SV=1)

HSP 1 Score: 600.5 bits (1547), Expect = 1.8e-170
Identity = 283/446 (63.45%), Postives = 352/446 (78.92%), Query Frame = 0

Query: 77  VPVFVMLPLDTVT-LGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWE 136
           VPVFVM+PLDTV+  G  LN+ +A+ ASL ALKSAGVEG+MVD WWG+VE +GP +YN++
Sbjct: 73  VPVFVMMPLDTVSKCGSALNRRKAVAASLAALKSAGVEGIMVDVWWGIVESEGPGRYNFD 132

Query: 137 GYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRR 196
           GY EL++M +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+ DL YTD+ GRR
Sbjct: 133 GYVELMEMARKTGLKVQAVMSFHQCGGNVGDSVNIPLPRWVVEEMEKDNDLAYTDQWGRR 192

Query: 197 NPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPS 256
           N EYISLGCD++PV +GRTP++ Y D+MR+FRD F  +LG+ I+E+QVG GP GELRYPS
Sbjct: 193 NFEYISLGCDAMPVFKGRTPVECYTDFMRAFRDHFASFLGDTIVEIQVGMGPAGELRYPS 252

Query: 257 YPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGF 316
           YP+SNGTWRFPGIG FQC D+YMR+SL+AAAE+ GK +WG +GGP D+G YN +PEDT F
Sbjct: 253 YPESNGTWRFPGIGAFQCNDRYMRSSLKAAAEARGKPEWG-HGGPTDAGGYNNWPEDTVF 312

Query: 317 FKKE-GTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIF-HGTGAKLSAKVAGIHWHYGT 376
           F+ + G W+TEYG+FFL WYS  LL+HG+R+L+ +  +F  G GAK+S KVAGIHWHYGT
Sbjct: 313 FRGDCGGWSTEYGEFFLSWYSQMLLEHGERVLSGATSVFGDGAGAKISVKVAGIHWHYGT 372

Query: 377 RSHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQ 436
           RSHA ELTAGYYNTRHR+GY PIA+MLA+HG V NFTC+EM+D +QP  A C PE LVRQ
Sbjct: 373 RSHAPELTAGYYNTRHRDGYLPIARMLARHGAVLNFTCVEMRDHEQPQEAQCMPEALVRQ 432

Query: 437 VKMATRDAKAELAGENALERYDGGAYEQILATSRSESG-NGLTAFTYLRMNKNLFEPNNW 496
           V  A R A   LAGENAL RYDG A++Q++A +   +  + + AFTYLRM  +LF P+NW
Sbjct: 433 VAAAARAAGVGLAGENALPRYDGTAHDQVVAAAADRAAEDRMVAFTYLRMGPDLFHPDNW 492

Query: 497 RNLVEFVKTMSEGGRNRKLPESDCSG 519
           R  V FV+ MSE G  R+  ES   G
Sbjct: 493 RRFVAFVRRMSESGSPREAAESAAHG 517

BLAST of CmaCh16G010940 vs. ExPASy Swiss-Prot
Match: O22585 (Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 3.5e-129
Identity = 222/434 (51.15%), Postives = 294/434 (67.74%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEG 136
           VPV+VMLPL  + +      P  +   L+ L++AGV+GVM+D WWG++E+ GP +Y+W  
Sbjct: 13  VPVYVMLPLGVINVNNVFEDPDGLKEQLVQLRAAGVDGVMIDVWWGIIEQKGPKEYDWSA 72

Query: 137 YAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRN 196
           Y  L Q++QK GLKLQ +MSFHQCGGNVGD  +IPLP WVL+    +PD+ YT+RSG RN
Sbjct: 73  YKSLFQLVQKCGLKLQAIMSFHQCGGNVGDVVNIPLPKWVLDIGESDPDIFYTNRSGIRN 132

Query: 197 PEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYL-GEVIMEVQVGAGPCGELRYPS 256
            EY+S+G D+ P+  GRT I++Y+DYM+SFR+   D L  EVI++++VG GP GELRYPS
Sbjct: 133 QEYLSIGVDNKPIFHGRTAIEIYSDYMKSFRENMSDLLKSEVIIDIEVGLGPAGELRYPS 192

Query: 257 YPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGF 316
           YPQ+ G W+FPGIGEFQCYDKY+R S +AAA   G  +W     P D+G YN  PE T F
Sbjct: 193 YPQNQG-WQFPGIGEFQCYDKYLRESFKAAAAKAGHSEWEL---PDDAGTYNDVPESTEF 252

Query: 317 FKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRS 376
           FK  GT+ TE GKFFL WYS +LL HGD+IL  +   F G   KL+ KV+GIHW Y   +
Sbjct: 253 FKTNGTYLTEKGKFFLTWYSNQLLNHGDQILDEANKAFLGCKVKLAIKVSGIHWWYKAPN 312

Query: 377 HAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVK 436
           HAAELTAGYYN   R+GY PIAK++++H  + NFTC+EM+D +Q   A+ SP+ LV+QV 
Sbjct: 313 HAAELTAGYYNLDDRDGYRPIAKIVSRHHAILNFTCLEMRDSEQSSDAHSSPQKLVQQVL 372

Query: 437 MATRDAKAELAGENALERYDGGAYEQILATSRSESGN-------GLTAFTYLRMNKNLFE 496
                   E+AGENAL RYD  AY QI+  +R +  N        +   TYLR++ +L +
Sbjct: 373 SGGWRENIEVAGENALSRYDATAYNQIILNARPQGVNKDGPPKLRMYGVTYLRLSDDLMQ 432

Query: 497 PNNWRNLVEFVKTM 503
            +N+    +FV  M
Sbjct: 433 QSNFDIFKKFVVKM 442

BLAST of CmaCh16G010940 vs. TAIR 10
Match: AT4G17090.1 (chloroplast beta-amylase )

HSP 1 Score: 812.4 bits (2097), Expect = 2.2e-235
Identity = 393/551 (71.32%), Postives = 457/551 (82.94%), Query Frame = 0

Query: 1   MTLTLRSSTSFINPKDNKIIKAHLHEDLYGVISFKQAKSST-RLRKRSSLQEIKM----- 60
           M LTL SS+S I  KD K   +   E     ++F + K  T + + ++S++E+K      
Sbjct: 1   MELTLNSSSSLIKRKDAK--SSRNQESSSNNMTFAKMKPPTYQFQAKNSVKEMKFTHEKT 60

Query: 61  -----ERSYKLEKLHSLSMNHHMNDLRVPVFVMLPLDTVTLGGNLNKPRAMNASLMALKS 120
                E   K EKLH LS  H  ND  VPVFVMLPLDTVT+ G+LNKPRAMNASLMALK 
Sbjct: 61  FTPEGETLEKWEKLHVLSYPHSKNDASVPVFVMLPLDTVTMSGHLNKPRAMNASLMALKG 120

Query: 121 AGVEGVMVDAWWGLVEKDGPMKYNWEGYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCS 180
           AGVEGVMVDAWWGLVEKDGPM YNWEGYAEL+QM+QKHGLKLQVVMSFHQCGGNVGDSCS
Sbjct: 121 AGVEGVMVDAWWGLVEKDGPMNYNWEGYAELIQMVQKHGLKLQVVMSFHQCGGNVGDSCS 180

Query: 181 IPLPPWVLEEISKNPDLVYTDRSGRRNPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDR 240
           IPLPPWVLEEISKNPDLVYTD+SGRRNPEYISLGCDS+PVLRGRTPIQVY+D+MRSFR+R
Sbjct: 181 IPLPPWVLEEISKNPDLVYTDKSGRRNPEYISLGCDSVPVLRGRTPIQVYSDFMRSFRER 240

Query: 241 FRDYLGEVIMEVQVGAGPCGELRYPSYPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESI 300
           F  Y+G VI E+QVG GPCGELRYPSYP+SNGTWRFPGIGEFQCYDKYM++SL+A AESI
Sbjct: 241 FEGYIGGVIAEIQVGMGPCGELRYPSYPESNGTWRFPGIGEFQCYDKYMKSSLQAYAESI 300

Query: 301 GKRDWGSNGGPHDSGQYNQFPEDTGFFKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAAS 360
           GK +WG++ GPHD+G+Y   PEDT FF+++GTWN+EYGKFF+EWYSGKLL+HGD++L+++
Sbjct: 301 GKTNWGTS-GPHDAGEYKNLPEDTEFFRRDGTWNSEYGKFFMEWYSGKLLEHGDQLLSSA 360

Query: 361 KGIFHGTGAKLSAKVAGIHWHYGTRSHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNF 420
           KGIF G+GAKLS KVAGIHWHY TRSHAAELTAGYYNTR+ +GY PIAKM  KHGVV NF
Sbjct: 361 KGIFQGSGAKLSGKVAGIHWHYNTRSHAAELTAGYYNTRNHDGYLPIAKMFNKHGVVLNF 420

Query: 421 TCMEMKDGQQPGYANCSPEGLVRQVKMATRDAKAELAGENALERYDGGAYEQILATSRSE 480
           TCMEMKDG+QP +ANCSPEGLV+QV+ ATR A  ELAGENALERYD  A+ Q++AT+RS+
Sbjct: 421 TCMEMKDGEQPEHANCSPEGLVKQVQNATRQAGTELAGENALERYDSSAFGQVVATNRSD 480

Query: 481 SGNGLTAFTYLRMNKNLFEPNNWRNLVEFVKTMSEGGRNRKLPESDCSGSDLHVGFIKEK 538
           SGNGLTAFTYLRMNK LFE  NW+ LVEFVK M EGG  R+L + D +GSDL+VGF+K K
Sbjct: 481 SGNGLTAFTYLRMNKRLFEGQNWQQLVEFVKNMKEGGHGRRLSKEDTTGSDLYVGFVKGK 540

BLAST of CmaCh16G010940 vs. TAIR 10
Match: AT3G23920.1 (beta-amylase 1 )

HSP 1 Score: 600.5 bits (1547), Expect = 1.3e-171
Identity = 288/468 (61.54%), Postives = 354/468 (75.64%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEG 136
           VPVFVM+PLD+VT+G  +N+ +AM ASL ALKSAGVEG+M+D WWGLVEK+ P  YNW G
Sbjct: 106 VPVFVMMPLDSVTMGNTVNRRKAMKASLQALKSAGVEGIMIDVWWGLVEKESPGTYNWGG 165

Query: 137 YAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRN 196
           Y EL+++ +K GLK+Q VMSFHQCGGNVGDS +IPLP WV+EE+ K+PDL YTD+ GRRN
Sbjct: 166 YNELLELAKKLGLKVQAVMSFHQCGGNVGDSVTIPLPQWVVEEVDKDPDLAYTDQWGRRN 225

Query: 197 PEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGEVIMEVQVGAGPCGELRYPSY 256
            EYISLG D+LPVL+GRTP+Q YAD+MR+FRD F+  LGE I+E+QVG GP GELRYPSY
Sbjct: 226 HEYISLGADTLPVLKGRTPVQCYADFMRAFRDNFKHLLGETIVEIQVGMGPAGELRYPSY 285

Query: 257 PQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGFF 316
           P+  GTW+FPGIG FQCYDKY  +SL+AAAE+ GK +WGS  GP D+G YN +PEDT FF
Sbjct: 286 PEQEGTWKFPGIGAFQCYDKYSLSSLKAAAETYGKPEWGST-GPTDAGHYNNWPEDTQFF 345

Query: 317 KKE-GTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRS 376
           KKE G WN+EYG FFL WYS  LL HG+RIL+++K IF   G K+S K+AGIHWHYGTRS
Sbjct: 346 KKEGGGWNSEYGDFFLSWYSQMLLDHGERILSSAKSIFENMGVKISVKIAGIHWHYGTRS 405

Query: 377 HAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQVK 436
           HA ELTAGYYNTR R+GY PIA+MLA+H  +FNFTC+EM+D +QP  A C+PE LV QV 
Sbjct: 406 HAPELTAGYYNTRFRDGYLPIAQMLARHNAIFNFTCIEMRDHEQPQDALCAPEKLVNQVA 465

Query: 437 MATRDAKAELAGENALERYDGGAYEQIL---ATSRSESGNG----LTAFTYLRMNKNLFE 496
           +AT  A+  LAGENAL RYD  A+EQIL   A +  ++  G    + AFTYLRMN  LF+
Sbjct: 466 LATLAAEVPLAGENALPRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELFQ 525

Query: 497 PNNWRNLVEFVKTMSEGGRNRKLPESDCSGSDLHVGFIKEKKIKEVAV 537
            +NW   V FVK M EG  + +  E +      H   + +  ++E AV
Sbjct: 526 ADNWGKFVAFVKKMGEGRDSHRCRE-EVEREAEHFVHVTQPLVQEAAV 571

BLAST of CmaCh16G010940 vs. TAIR 10
Match: AT4G15210.1 (beta-amylase 5 )

HSP 1 Score: 443.7 bits (1140), Expect = 2.1e-124
Identity = 216/435 (49.66%), Postives = 285/435 (65.52%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALK-SAGVEGVMVDAWWGLVEKDGPMKYNWE 136
           VPV+VMLPL  V +      P  +   L  LK  AGV+GVMVD WWG++E  GP +Y+W 
Sbjct: 14  VPVYVMLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWT 73

Query: 137 GYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRR 196
            Y  L Q++ + GLK+Q +MSFHQCGGNVGD  +IP+P WV +    +PD+ YT+R G R
Sbjct: 74  AYKTLFQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTR 133

Query: 197 NPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYL-GEVIMEVQVGAGPCGELRYP 256
           + EY+S+G D+LP+  GRT +Q+Y+DYM SF++   D +   VI++++VG GP GELRYP
Sbjct: 134 DIEYLSIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYP 193

Query: 257 SYPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTG 316
           SYPQS G W FPGIGEFQCYDKY++   + AA   G  +W     P D+G+YN  PE+TG
Sbjct: 194 SYPQSQG-WVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEWDL---PEDAGEYNDKPEETG 253

Query: 317 FFKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTR 376
           FFKK+GT+ +E GKFF+ WYS KL+ HGD+IL  +  IF G    L+AKV+GIHW Y   
Sbjct: 254 FFKKDGTYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHH 313

Query: 377 SHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQV 436
           SHAAELTAGYYN   R+GY PIA+ML+KH  + NFTC+EMKD      A  +P+ LV++V
Sbjct: 314 SHAAELTAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEV 373

Query: 437 KMATRDAKAELAGENALERYDGGAYEQILATSRSESGN-------GLTAFTYLRMNKNLF 496
                    E+AGENALE Y    Y QIL  +R    N        +  FTYLR++  +F
Sbjct: 374 LSKAWKEGIEVAGENALETYGAKGYNQILLNARPNGVNPNGKPKLRMYGFTYLRLSDTVF 433

Query: 497 EPNNWRNLVEFVKTM 503
           + NN+    + V+ M
Sbjct: 434 QENNFELFKKLVRKM 444

BLAST of CmaCh16G010940 vs. TAIR 10
Match: AT4G00490.1 (beta-amylase 2 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-121
Identity = 219/433 (50.58%), Postives = 280/433 (64.67%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALKSAGVEGVMVDAWWGLVEKDGPMKYNWEG 136
           VPV+VMLPL  + +   + +P  +   L  LKS  V+GVMVD WWG+VE   P  YNW G
Sbjct: 95  VPVYVMLPLGVIDMNSEVVEPEELLDQLRTLKSVNVDGVMVDCWWGIVESHTPQVYNWSG 154

Query: 137 YAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRRN 196
           Y +L QM+++ GLK+QVVMSFH+CGGNVGD   I +P WV E    NPD+ +TD +GRRN
Sbjct: 155 YKKLFQMIRELGLKIQVVMSFHECGGNVGDDVHIQIPEWVREIGQSNPDIYFTDSAGRRN 214

Query: 197 PEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYLGE-VIMEVQVGAGPCGELRYPS 256
            E ++ G D   VLRGRT ++VY DYMRSFR  F ++  E +I E++VG GPCGELRYPS
Sbjct: 215 TECLTWGIDKQRVLRGRTALEVYFDYMRSFRVEFDEFFEEKIIPEIEVGLGPCGELRYPS 274

Query: 257 YPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTGF 316
           YP   G W++PGIGEFQCYDKY+  SL+ AAE  G   WG   GP ++  YN  P  TGF
Sbjct: 275 YPAQFG-WKYPGIGEFQCYDKYLMNSLKEAAEVRGHSFWGR--GPDNTETYNSTPHGTGF 334

Query: 317 FKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTRS 376
           F+  G +++ YG+FFL WYS  L+ HGDR+LA +   F GT   ++AK++GIHW Y T S
Sbjct: 335 FRDGGDYDSYYGRFFLNWYSRVLIDHGDRVLAMANLAFEGT--CIAAKLSGIHWWYKTAS 394

Query: 377 HAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQP---GYANCSPEGLVR 436
           HAAELTAG+YN+ +R+GY PIA M  KH    NFTC+E++   Q      A   PEGLV 
Sbjct: 395 HAAELTAGFYNSSNRDGYGPIAAMFKKHDAALNFTCVELRTLDQHEDFPEALADPEGLVW 454

Query: 437 QVKMATRDAKAELAGENALERYDGGAYEQILATSR---SESGNGLTAFTYLRMNKNLFEP 496
           QV  A  DA   +A ENAL  YD   Y +IL  ++      G  L+ FTYLR+N  L E 
Sbjct: 455 QVLNAAWDASIPVASENALPCYDREGYNKILENAKPLTDPDGRHLSCFTYLRLNPTLMES 514

Query: 497 NNWRNLVEFVKTM 503
            N++    F+K M
Sbjct: 515 QNFKEFERFLKRM 522

BLAST of CmaCh16G010940 vs. TAIR 10
Match: AT4G15210.2 (beta-amylase 5 )

HSP 1 Score: 429.9 bits (1104), Expect = 3.1e-120
Identity = 206/398 (51.76%), Postives = 267/398 (67.09%), Query Frame = 0

Query: 77  VPVFVMLPLDTVTLGGNLNKPRAMNASLMALK-SAGVEGVMVDAWWGLVEKDGPMKYNWE 136
           VPV+VMLPL  V +      P  +   L  LK  AGV+GVMVD WWG++E  GP +Y+W 
Sbjct: 14  VPVYVMLPLGVVNVENVFADPETLETQLKRLKEEAGVDGVMVDVWWGIIESKGPKQYDWT 73

Query: 137 GYAELVQMLQKHGLKLQVVMSFHQCGGNVGDSCSIPLPPWVLEEISKNPDLVYTDRSGRR 196
            Y  L Q++ + GLK+Q +MSFHQCGGNVGD  +IP+P WV +    +PD+ YT+R G R
Sbjct: 74  AYKTLFQLIARLGLKIQAIMSFHQCGGNVGDIVTIPIPQWVRDVGDNDPDIYYTNRKGTR 133

Query: 197 NPEYISLGCDSLPVLRGRTPIQVYADYMRSFRDRFRDYL-GEVIMEVQVGAGPCGELRYP 256
           + EY+S+G D+LP+  GRT +Q+Y+DYM SF++   D +   VI++++VG GP GELRYP
Sbjct: 134 DIEYLSIGVDNLPLFAGRTAVQLYSDYMSSFKENMADLIEAGVIVDIEVGLGPAGELRYP 193

Query: 257 SYPQSNGTWRFPGIGEFQCYDKYMRASLEAAAESIGKRDWGSNGGPHDSGQYNQFPEDTG 316
           SYPQS G W FPGIGEFQCYDKY++   + AA   G  +W     P D+G+YN  PE+TG
Sbjct: 194 SYPQSQG-WVFPGIGEFQCYDKYLKKDFKEAAAKAGHPEWDL---PEDAGEYNDKPEETG 253

Query: 317 FFKKEGTWNTEYGKFFLEWYSGKLLQHGDRILAASKGIFHGTGAKLSAKVAGIHWHYGTR 376
           FFKK+GT+ +E GKFF+ WYS KL+ HGD+IL  +  IF G    L+AKV+GIHW Y   
Sbjct: 254 FFKKDGTYVSEKGKFFMTWYSNKLIFHGDQILGEANKIFAGLKVNLAAKVSGIHWLYNHH 313

Query: 377 SHAAELTAGYYNTRHRNGYSPIAKMLAKHGVVFNFTCMEMKDGQQPGYANCSPEGLVRQV 436
           SHAAELTAGYYN   R+GY PIA+ML+KH  + NFTC+EMKD      A  +P+ LV++V
Sbjct: 314 SHAAELTAGYYNLFKRDGYRPIARMLSKHYGILNFTCLEMKDTDNTAEALSAPQELVQEV 373

Query: 437 KMATRDAKAELAGENALERYDGGAYEQILATSRSESGN 473
                    E+AGENALE Y    Y QIL  +R    N
Sbjct: 374 LSKAWKEGIEVAGENALETYGAKGYNQILLNARPNGVN 407

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O235533.1e-23471.32Beta-amylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM3 PE=1 SV=3[more]
Q10RZ16.4e-17164.20Beta-amylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY2 ... [more]
Q9LIR61.8e-17061.54Beta-amylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BAM1 PE=1 SV=1[more]
Q9AV881.8e-17063.45Beta-amylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=BAMY1 ... [more]
O225853.5e-12951.15Beta-amylase OS=Medicago sativa OX=3879 GN=BMY1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17090.12.2e-23571.32chloroplast beta-amylase [more]
AT3G23920.11.3e-17161.54beta-amylase 1 [more]
AT4G15210.12.1e-12449.66beta-amylase 5 [more]
AT4G00490.11.6e-12150.58beta-amylase 2 [more]
AT4G15210.23.1e-12051.76beta-amylase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001554Glycoside hydrolase, family 14PRINTSPR00750BETAAMYLASEcoord: 375..386
score: 85.42
coord: 246..268
score: 57.17
coord: 132..150
score: 43.16
coord: 154..175
score: 72.5
coord: 111..125
score: 72.0
coord: 393..416
score: 51.67
coord: 430..452
score: 44.78
coord: 358..374
score: 46.47
coord: 322..341
score: 59.75
IPR001554Glycoside hydrolase, family 14PFAMPF01373Glyco_hydro_14coord: 80..498
e-value: 1.5E-167
score: 557.7
IPR001554Glycoside hydrolase, family 14PANTHERPTHR31352FAMILY NOT NAMEDcoord: 1..534
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 69..533
e-value: 4.4E-214
score: 713.4
NoneNo IPR availablePANTHERPTHR31352:SF1BETA-AMYLASE 3, CHLOROPLASTICcoord: 1..534
IPR018238Glycoside hydrolase, family 14, conserved sitePROSITEPS00506BETA_AMYLASE_1coord: 158..166
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 76..505

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G010940.1CmaCh16G010940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000272 polysaccharide catabolic process
molecular_function GO:0102229 amylopectin maltohydrolase activity
molecular_function GO:0016161 beta-amylase activity