CmaCh16G010880 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010880
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	protein N-lysine methyltransferase METTL21A-like
Location	Cma_Chr16: 8367693 .. 8368457 (-)
RNA-Seq Expression	CmaCh16G010880
Synteny	CmaCh16G010880

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAGAGAAAAAAGGGGGATGGTTTTTGCTATAAAAGAAGGCACAAAGAAGACTGGAGCTGCAAAGGAAAAGGGCAAGGAGATTTCTATGGGCGTTAGAGAATTCCAAATCAGTGATAGAAACGTTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACCGGTAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCAGCCTTCGTCCTAGCCGAATGGATGGCAACTCAGTGCAAGTTATTCGACTTCGACATCCGGGACAAGACCGTGATTGAGCTCGGAGCTGGGGTTGGACTCCCTGGTTTGATGGCAGCTTTACTTGGTGCTCGTCGTGTTGTACTTACTGATGTAGAACCATTGCTTCCTGGGTTGTTGAGGAATGTAGATGCCAATGGACTCGTGGACCGAGTTCAAGTAAGGGAACTCGTCTGGGGCTTAGATGAATCAGTGAGTCGAGCAAACGAGTTGGGAGAGTTTGACCTGATTTTGATGTCGGATTTGTTCTATGACTCAGAGGACATGGCCCCTCTAGCACACGTCCTAAAGATCATCTCTGGGAAAAATAGCAAAATCTGGGCAGCTTCTGAGATTCGACCATGGACCGTCGAGTGCCTAAAAGAGTTCATGAATGAAGGATTCAAGGTCGAAGAGTCACAAGTTCAACTAGACGAACCTCGTGAGGAAGACGACGACCACGCCATTTTAGACTTGTTTTCTATTTTCCATCTCCAACCACCTGATGAAGAAAGGAACTCCTAA

mRNA sequence

ATGACGAGAGAAAAAAGGGGGATGGTTTTTGCTATAAAAGAAGGCACAAAGAAGACTGGAGCTGCAAAGGAAAAGGGCAAGGAGATTTCTATGGGCGTTAGAGAATTCCAAATCAGTGATAGAAACGTTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACCGGTAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCAGCCTTCGTCCTAGCCGAATGGATGGCAACTCAGTGCAAGTTATTCGACTTCGACATCCGGGACAAGACCGTGATTGAGCTCGGAGCTGGGGTTGGACTCCCTGGTTTGATGGCAGCTTTACTTGGTGCTCGTCGTGTTGTACTTACTGATGTAGAACCATTGCTTCCTGGGTTGTTGAGGAATGTAGATGCCAATGGACTCGTGGACCGAGTTCAAGTAAGGGAACTCGTCTGGGGCTTAGATGAATCAGTGAGTCGAGCAAACGAGTTGGGAGAGTTTGACCTGATTTTGATGTCGGATTTGTTCTATGACTCAGAGGACATGGCCCCTCTAGCACACGTCCTAAAGATCATCTCTGGGAAAAATAGCAAAATCTGGGCAGCTTCTGAGATTCGACCATGGACCGTCGAGTGCCTAAAAGAGTTCATGAATGAAGGATTCAAGGTCGAAGAGTCACAAGTTCAACTAGACGAACCTCGTGAGGAAGACGACGACCACGCCATTTTAGACTTGTTTTCTATTTTCCATCTCCAACCACCTGATGAAGAAAGGAACTCCTAA

Coding sequence (CDS)

ATGACGAGAGAAAAAAGGGGGATGGTTTTTGCTATAAAAGAAGGCACAAAGAAGACTGGAGCTGCAAAGGAAAAGGGCAAGGAGATTTCTATGGGCGTTAGAGAATTCCAAATCAGTGATAGAAACGTTGTAATCCATGAGCTTGACGACCTCTATGATTCCACAACCGGTAGAGTGCTCACCGGTTCCTGGGTCTGGAACTCAGCCTTCGTCCTAGCCGAATGGATGGCAACTCAGTGCAAGTTATTCGACTTCGACATCCGGGACAAGACCGTGATTGAGCTCGGAGCTGGGGTTGGACTCCCTGGTTTGATGGCAGCTTTACTTGGTGCTCGTCGTGTTGTACTTACTGATGTAGAACCATTGCTTCCTGGGTTGTTGAGGAATGTAGATGCCAATGGACTCGTGGACCGAGTTCAAGTAAGGGAACTCGTCTGGGGCTTAGATGAATCAGTGAGTCGAGCAAACGAGTTGGGAGAGTTTGACCTGATTTTGATGTCGGATTTGTTCTATGACTCAGAGGACATGGCCCCTCTAGCACACGTCCTAAAGATCATCTCTGGGAAAAATAGCAAAATCTGGGCAGCTTCTGAGATTCGACCATGGACCGTCGAGTGCCTAAAAGAGTTCATGAATGAAGGATTCAAGGTCGAAGAGTCACAAGTTCAACTAGACGAACCTCGTGAGGAAGACGACGACCACGCCATTTTAGACTTGTTTTCTATTTTCCATCTCCAACCACCTGATGAAGAAAGGAACTCCTAA

Protein sequence

MTREKRGMVFAIKEGTKKTGAAKEKGKEISMGVREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDANGLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECLKEFMNEGFKVEESQVQLDEPREEDDDHAILDLFSIFHLQPPDEERNS

Homology

[Close]

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 4.0e-14
Identity = 62/168 (36.90%), Postives = 89/168 (52.98%), Query Frame = 0


Query: 65  VWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLP 124
           VW++A VL  ++    ++   D++ K VIELGAG GL G++AALLGA  V +TD EP L 
Sbjct: 46  VWDAAVVLCMFL----EMGKVDLKGKRVIELGAGTGLVGIVAALLGA-NVTITDREPALE 105

Query: 125 GLLRNVDAN---GLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAH 184
            L  NV  N   G    VQV EL WG  E++    + G +DLIL +D+ Y  E    L  
Sbjct: 106 FLTANVHENIPQGRQKAVQVSELTWG--ENLDLYPQ-GGYDLILGADIVYLEETFPALLQ 165

Query: 185 VLKIISGKNSKIWAASEIRPWTVECLKEFMNEGFKVEESQVQLDEPRE 230
            L+ +S  ++ +  +  IR    E     + + F V+E  V  D  R+
Sbjct: 166 TLEHLSSGDTVVLLSCRIRYERDERFLTELRQRFSVQE--VHYDSQRD 203

[Close]

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot
Match: A4FV98 (EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 5.2e-14
Identity = 57/165 (34.55%), Postives = 88/165 (53.33%), Query Frame = 0


Query: 65  VWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLP 124
           VW++A  L  +  +Q    + D R K VIELGAG G+ G++AAL G   V +TD+  +L 
Sbjct: 56  VWDAALSLCNYFESQ----NVDFRGKKVIELGAGTGIVGILAALQGG-DVTITDLPLVLE 115

Query: 125 GLLRNVDAN-GLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAHVL 184
            +  NV AN     R QVR L WG+D+ V      G++DL+L +D+ Y       L   L
Sbjct: 116 QIQGNVQANVPPGGRAQVRALSWGIDQHVFP----GDYDLVLGADIVYLEPTFPLLLGTL 175

Query: 185 KIISGKNSKIWAASEIRP--WTVECLKEFMNEGFKVEESQVQLDE 227
           + + G +  I+ AS++R    T    +  + + F++E +Q   DE
Sbjct: 176 RHLCGPHGTIYLASKMREEHGTESFFQHLLPQHFQLELAQRDEDE 211

[Close]

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot
Match: A4FV42 (Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE=2 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 3.4e-13
Identity = 53/139 (38.13%), Postives = 78/139 (56.12%), Query Frame = 0


Query: 65  VWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLP 124
           VW++A VL    AT  ++   ++R  + +ELGAG GL G++AALLGA  V +TD +  L 
Sbjct: 46  VWDAAVVL----ATYLEMGTVELRGCSAVELGAGTGLVGIVAALLGA-HVTITDRKVALE 105

Query: 125 GLLRNVDAN---GLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAH 184
            L  NV AN    +  +  V+EL WG  +++ R +  GEFDLIL +D+ Y  E    L  
Sbjct: 106 FLKSNVQANLPPHIQPKAVVKELTWG--QNLGRFSP-GEFDLILGADIIYLEETFTDLLQ 165

Query: 185 VLKIISGKNSKIWAASEIR 201
            L+ +   +S +  A  IR
Sbjct: 166 TLEHLCSNHSVVLLACRIR 176

[Close]

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot
Match: Q8CDZ2 (Protein-lysine methyltransferase METTL21E OS=Mus musculus OX=10090 GN=Mettl21e PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.4e-13
Identity = 50/122 (40.98%), Postives = 72/122 (59.02%), Query Frame = 0


Query: 55  TTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRV 114
           T G+   G++VW SA VL  ++ T  K   +++ DK VIE+GAG GL  ++A+LLGA RV
Sbjct: 58  TEGKDCYGAFVWPSALVLCYFLETHAK--QYNMVDKNVIEIGAGTGLVSIVASLLGA-RV 117

Query: 115 VLTDVEPLLPGLLRNVDANGLV---DRVQVRELVWG--LDESVSRANELGEFDLILMSDL 172
           + TD+  LL  L  N+  N  +      QV+EL WG  LD +  R++    FD IL +D+
Sbjct: 118 IATDLPELLGNLQYNISRNTKMKCKHLPQVKELSWGVALDRNFPRSS--NNFDYILAADV 174

[Close]

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 7.5e-13
Identity = 52/139 (37.41%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 65  VWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLP 124
           VW++A VL    +T  ++   ++R ++ +ELGAG GL G++AALLGA  V +TD +  L 
Sbjct: 46  VWDAAIVL----STYLEMGAVELRGRSAVELGAGTGLVGIVAALLGA-HVTITDRKVALE 105

Query: 125 GLLRNVDAN---GLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAH 184
            L  NV AN    +  +  V+EL WG +     +   GEFDLIL +D+ Y  E    L  
Sbjct: 106 FLKSNVQANLPPHIQTKTVVKELTWGQNLG---SFSPGEFDLILGADIIYLEETFTDLLQ 165

Query: 185 VLKIISGKNSKIWAASEIR 201
            L+ +   +S I  A  IR
Sbjct: 166 TLEHLCSNHSVILLACRIR 176

[Close]

BLAST of CmaCh16G010880 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-14
Identity = 55/190 (28.95%), Postives = 94/190 (49.47%), Query Frame = 0


Query: 43  VVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFD---IRDKTVIELGAGV 102
           V+ HELD   D  +  +  G+ VW+++ V A+++    +   F    ++ K  IELGAG 
Sbjct: 18  VLGHELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGC 77

Query: 103 GLPGLMAALLGARRVVLTDVEPLLPGLLRNVDAN----------GLVDRVQVRELVWGLD 162
           G+ G   A+LG   VV TD + +LP L RNV+ N               ++V EL WG +
Sbjct: 78  GVAGFALAMLGC-DVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNE 137

Query: 163 ESVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTV-ECLK 219
           + ++       FD ++ +D+ Y  + + PL   +  +SG  + +    EIR   V E + 
Sbjct: 138 DHITAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKML 197

[Close]

BLAST of CmaCh16G010880 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.8e-14
Identity = 55/190 (28.95%), Postives = 94/190 (49.47%), Query Frame = 0


Query: 43  VVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFD---IRDKTVIELGAGV 102
           V+ HELD   D  +  +  G+ VW+++ V A+++    +   F    ++ K  IELGAG 
Sbjct: 25  VLGHELDFAQDPNSKHL--GTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGC 84

Query: 103 GLPGLMAALLGARRVVLTDVEPLLPGLLRNVDAN----------GLVDRVQVRELVWGLD 162
           G+ G   A+LG   VV TD + +LP L RNV+ N               ++V EL WG +
Sbjct: 85  GVAGFALAMLGC-DVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNE 144

Query: 163 ESVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTV-ECLK 219
           + ++       FD ++ +D+ Y  + + PL   +  +SG  + +    EIR   V E + 
Sbjct: 145 DHITAVEP--PFDYVIGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKML 204

[Close]

BLAST of CmaCh16G010880 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-11
Identity = 46/145 (31.72%), Postives = 69/145 (47.59%), Query Frame = 0


Query: 60  LTGSWVWNSAFVLAEWMATQCKLFDFDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDV 119
           +TGS +W+S  VL +++          +  K ++ELG+G GL G +AALLG    VLTD+
Sbjct: 117 VTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGCIAALLGG-NAVLTDL 176

Query: 120 EPLLPGLLRNVDAN----GLVDRVQVRELVWGLDESVSRANELGEFDLILMSDLFYDSED 179
              L  L +N+  N           V+ELVWG D            D +L SD+ Y  E 
Sbjct: 177 PDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDDPDPDLIEPFP--DYVLGSDVIYSEEA 236

Query: 180 MAPLAHVLKIISGKNSKIWAASEIR 201
           +  L   L  +    + I+ + E+R
Sbjct: 237 VHHLVKTLLQLCSDQTTIFLSGELR 258

[Close]

BLAST of CmaCh16G010880 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 4.7e-10
Identity = 43/154 (27.92%), Postives = 73/154 (47.40%), Query Frame = 0


Query: 85  FDIRDKTVIELGAGVGLPGLMAALLGARRVVLTDVEPLLPGLLRNVDAN--GLVDRVQVR 144
           FD R +  IELG G G+ G+   LLG   +VLTD+ P++P L  N+  N   L   ++  
Sbjct: 66  FDFRRRRGIELGTGCGVAGMAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGKSLKTS 125

Query: 145 ELVWGLDESVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIR-P 204
            + W   + +S       FDL++ +D+ Y  E +  L   ++++   +  +    +IR P
Sbjct: 126 IVYWNNRDQISALKP--PFDLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSP 185

Query: 205 WTVECLKEFMNEGFKVEE---SQVQLDEPREEDD 233
              +   E  +  FK+E+     +  D   EE D
Sbjct: 186 EADKLFWELCDIVFKIEKVPHEHLHSDYAYEETD 217

[Close]

BLAST of CmaCh16G010880 vs. TAIR 10
Match: AT4G35987.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.2e-09
Identity = 59/186 (31.72%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 33  VREFQISDRNVVIHELDDLYDSTTGRVLTGSWVWNSAFVLAEWMATQCKLFDFDIRDKTV 92
           + +F+IS+R    + +D+     TG V      W S  VLA +  +Q + F    R K V
Sbjct: 89  LNDFEISNR----YNIDN-----TGLVCQ----WPSEEVLAYFCKSQPERF----RGKRV 148

Query: 93  IELGAGVGLPGL-MAALLGARRVVLTDVEPLLPGLL-RNVDANGLV---DRVQVRELVWG 152
           IELG+G GL GL +AA   A  VV++D  P +   + RN++ N +      V+  EL W 
Sbjct: 149 IELGSGYGLAGLVIAAATEASEVVISDGNPQVVNYIKRNIETNSMAFGGTSVKAMELHWN 208

Query: 153 LDESVSRANELGEFDLILMSDLFYDSEDMAPLAHVLKIISGKNSKIWAASEIRPWTVECL 212
             +     N    FD+I+ SD  +  E    LA  +K++  K  K   A    P   + L
Sbjct: 209 QHQLSELTN---TFDIIVASDCTFFKEFHKDLARTIKMLL-KAKKASEALFFSPKRGDSL 253

Query: 213 KEFMNE 214
           ++FM E
Sbjct: 269 EKFMKE 253

The following BLAST results are available for this feature:

BLAST of CmaCh16G010880 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5BLD8	4.0e-14	36.90	Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P...	[more]
A4FV98	5.2e-14	34.55	EEF1A lysine methyltransferase 3 OS=Bos taurus OX=9913 GN=EEF1AKMT3 PE=2 SV=1	[more]
A4FV42	3.4e-13	38.13	Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE...	[more]
Q8CDZ2	4.4e-13	40.98	Protein-lysine methyltransferase METTL21E OS=Mus musculus OX=10090 GN=Mettl21e P...	[more]
Q8WXB1	7.5e-13	37.41	Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ...	[more]

BLAST of CmaCh16G010880 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G08125.1	1.8e-14	28.95	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G08125.2	1.8e-14	28.95	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G73320.1	3.2e-11	31.72	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G44170.1	4.7e-10	27.92	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G35987.1	5.2e-09	31.72	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 6..242 e-value: 3.0E-43 score: 150.0
None	No IPR available	PANTHER	PTHR14614:SF123	OSJNBA0070O11.4 PROTEIN	coord: 31..249
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 91..175 e-value: 7.82684E-5 score: 38.9503
IPR019410	Lysine methyltransferase	PFAM	PF10294	Methyltransf_16	coord: 61..211 e-value: 9.3E-27 score: 93.9
IPR019410	Lysine methyltransferase	PANTHER	PTHR14614	HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN	coord: 31..249
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 68..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010880.1	CmaCh16G010880.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
molecular_function	GO:0008168	methyltransferase activity