CmaCh16G010780 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010780
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	beta-amyrin 11-oxidase-like
Location	Cma_Chr16: 8252618 .. 8258156 (-)
RNA-Seq Expression	CmaCh16G010780
Synteny	CmaCh16G010780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAACACCAAGGGGCAATACAAAAGTGTGTTCTTAAGGAAAGGGATCCAAAGCAAAGGTGTTAGCTGCAGAAATCAATGGAGTTCATAAATGGGGTTTTCTTGATTCTCCCTGTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTCCCTCCTGGTGATCTCTGTTGGCCACTTCTTGGCTCTACTTTATCCTTCCTCAAATCTTTCACCGTTGGTCCACCCGAAAGCTTTATCCGTGCCTTTACCACCAGGTCTTTCTCCCTCTCGTGAGTTATTTGAAAACTCCTTTTAAATCTTTTCGGTAAGATATATACTCTCGTTCATGTCTATTTGATTATAATTCAAAGAAATTTTTTGTCATTCTAATTTTCTAAGAATTATTGATACCGTTGGTTGTTGGTTGTTGGTTTGGAATTACTTTCTAAATTCTTAAAGAAAAAATTTTGGTTTGCAAATTTTTATTATGGACTTAAAAGTCAATCCAATCAAGTTATTGATTTTGTAGTTATAAACAAATGAAGTGTTGGAAAGGGTTGACTTCAAACATTATTATTATTATTATTATTATTTTTTAAAGTGGAGCGTTTGAACTTACGAGGATAACCGTGCACATAAATTTAATAGGAGAGGGACCAATCCAAGCATGTCTTCGTTTTATAGTTACAAATTATTGAGGCGTTAGAAAGAGAATTGACTTTAAACATTATGTTATTTGGATATCTAATCTATAAAATAACCAACCATACACTCAAGTATAATAGAAACAAAATCAAACTCTAGCTGTTTTGATGGACATATAATACATATTTATGTAAATTAAACCTATCAAACACAAAATTGAATCGGGACAAATTGATTTCATTATAATTTTGAAACTACAAAAGCTTGATACGAAGTTTAGGAACCAAACGGTTAATGACCGTTTTTTTTCTTGTTAATGTTTATTGAGGTAGGTATGGGAAAGTTGACATGTACAAGACACACATTTTTGGGAGGGCAAGCATACTTGTCTGCAAGCCTGAAATCTGTCGCCAAGTACTAAGCGATGAAACCATATTTGTTCCTGGTTATCCCGCCAGCATGCAAACGTTATTTGGTAGAAATACGCTGCACCGAGCCTCAAAGGCGGAGCACCGGAAGCTCCGACGGGTGACAACGGCGCCGATCAGTAGCCACGCTGCGCTCGAGATGTACGTCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATAATGATATTTCACTTGGGTTCTTCTCTTTGCCCATCAACTTTCCTGGCTTCTACTTCCATAAATCTCTCAAGGTAAGAACTTTTTCCTTCTAAGTATGTATATTAGATGAAGAGTGTTGTTTAACAATTAGAACGCTGTTCAATAAGCTAAAACATTATACAATCTGCGGAAAGGTTATAGATTTAAATCTCAATCGTATATGTCCCAACTCAAAAATACTTTCAAACCTATGATGTTTAGGTGATCTGTTTATGAATGGCAACTTTTGAAAAAATTTCAGTATTTTTTAAGCAAATCTTTTATCATGCAACCCGTCAGCCTCACGATTTTAAAGCACGTCTATTATTAGGAAGAGATTTTCACACTCTTATAAAGAATGTTTTTTTCCCCTCTCCAACGGATGTGGGATCTCACAATCTATTCCCTTTGCAGCCCAGTGTCCTCACTTGCACACCGGTTGGTGTGTGGTTCTGACATCATTTGTAATAGCCCAAGTCTACCGCTAGTAGATATTGTCCACTTTGGCCCGTTACGAATCGCCGTAAACCTCATAGTATTAAAATGTGTATGCTAGGGAGAGGTTTTCACACCCATATAAGGAATGTTTTGCTCCCCTCTCCAACTAATGTGAGATTTCACAATCCACCCCCTTGGAGCCTAGCATTTTCGCTGACTCACCACCCGGTGTCTAACTCTAATACCATTTCTAACTGTCCAAGCCCACCACGAGCAAATATTGTCCGCTTTGGTCTGTTACGTATCACCGTCAACCTCACGGTTTTAAATTGCATCTGTTAGGGAGAAGTTTTCACATCCATATAAGAAATGCTTTGTTCCCCGCTCCAACCAACGTGGGATCTCACACAAAAATAAATGTATGAATACAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAATGAGAAGAGGTTGATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCACAAGATATGATGGATTTGATGATAGAAGTGGAAGATGAAGATGGAGAAGGGATGGACAATGAGACCATTGCAGATTTGATTTTTGGTAAGTTATTTGCAGGCCAGGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATCTGTTCCAAAAGGCCAAGGTTAGAGTTCTTTAATTTTCTAACTTGTTAATTGCATTCATTTGTCTCAATCGTATGATATCTTTATTTATCTAAGTTATTGCATATTAATTTTCTCTTGAATGCGTTTGTGGTTTGAAATAAGAATATTTGGCTCGTATGTTAGAGTTGTGTAGTCTAACGTTAAGAAATCTACTTGTTTATCCAAAATGATGTTCAAGTAAAAAAATGGTATATGAATATATGAGTTCATATCTAAATGAGAGTGATTTTAAAAATATAATAATTAATAAGGGCATAAAAACATAAAAACACATGTAATTTTGAAATTCTTCTGGGTAACACTTTCCCTCTTTATTAAAATCAATTAAGAAATACCAATTAAGAAAAGATTACACGAGATCATATGTTGAAACGACTTAAATACCAATTAAGAAAAGATTACAAGAGACAATATGTAGAAACGACCGAACTCTGTCGTGCTTGAAATTGCTTGAGATTTTTTTGGATAAAAACTTACTAGGTTGGAAGGTATTGATAATAGATGCTATACAATTTATTTCTTAAATATCTAAATATTTATTCATAAAATGCTTAGATATGAATATGGAGTCTAAATTATTTTCCTAAAATATCTGGATATCTTTGTTCTGAAATACTAGATTATTTCCATATACAATGGGTTAATACAATTTTTGGACTTGACCATATTCAGCTAGTGATATATCTTAACACTAACCTCTTGAAAATTTTCCTATCAACATATGATTGAGGAAAAACTTGCTAAAAAATTTCACGACAATTAGGGAATGTGATAATGTCCTACGAAATACAACTAAATATTGGGGTCATTAGTTAACTTATGAATCCTAATCTGATTTGTGAGGTCTTTTGTTGCAATGAACTTGGATTTTATTTTCTATTTTTGTTTCAAAAAGTTAATTAAGCTACAAGTTTTAGAACAAGAAACAAATAAGAAAGGCTAGAATTGGCTCGTGTATGTAGATTTATGTTCATATAAATCTTAATCCCTTCAAAAAGCCAAGTGAAATTTAAGATATTACAACAATATTGTCATATACTTCTTTCTAATATCATGTTCATTCCTCCGAATTTTATCATTTTGATAGTAATTTTTAAGATTAATTTGTTTTAGGAAGAACAAGAAGATATCATTAGTCGAAGACCTTCAACACAAAAGGGTATAAATCTCGCTGAATTTAAACAAATGAAATTTCTTTCTCAGGTAAATTTTTTCTTCAATTTTAGTTACGATGTTTAAATAAACTGTATATCTAAGATATTGTAATATCCAGGTGATAGATGAGACGCTTCGTCTTAGTGGTATTATATTTGCTACTTTTCGTGAGGCAACAACTGACGTTAATATCAATGGTTAGTCATCAACAAAGAAGGCTCTCTTTAAATTATGATCTTTCATGGATCTTAATCATGTTTTAGGTAAGCTTATACCAAAAGGATGGAAAGTCATACTTTGGCTTCGAGAGCTTTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCAAGATGGGATGTACGCAATCACTTTCCATATTTGCTGTCCTTTATTATTTTTGTTACTTAAAATCATTGTCTACATGCAATAAATATAGGCCTAAACTAATGGAAAGAAGAAAGAATATCATTTTAGTCTTTAAGTATAAGAGATCACGTCCATCCTTCTTAGATATTGTCTTCTTTGACTTGTTATGTATCGGTGTCAGCGTCACAATTTTAAAATGTTTCTGCTAGAGAGAGATTTCCACACTGTTATAAGAAATGTTTCGTTCCCTCTTCAACCAATGTGGGATCTCATACTAAGTTTTAAATACAATTTCTATTTCATCCTTCAATTTCAAAATGTTGCATTTTTACACTTTTACTCATATCGGTAAATAATGCTCATTTTTATGTCAAGTCTTTGACTTTTGGTTAAGTAGTTTAAAATAGTTATGATGTGGATGGTTAAAGTGAACCTAGCTCAAAATTCTGAAAAGTCATGAATAAAAGTGTAAGATCTCAAATCTTATAACCAAATGAAAACTAGACTCATAATTCTAGGGTAAAGATGTAATATTTTAAAATTTAATGAGAAAAATTGAAACTAAACTCAAAATTGAAGGATAAAAATGTAACATTTTGAAACGTATAAATTAAATGGAAACTATACTCAAAATTCGGGGACGAAAAATGTATTTTTCTCTCCCGTTGTTAAACTATTTATAACCTTTAATGTTGTTTCTTACTATTTGATTATTTATGTTGAACAGAATTTTATAGGCAACCCAGGTGCTTTTACCCCGTTCGGATTAGGAGTCAGGATGTGCCCTAGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGTGAGTTTGCTCAAACTTTTCTTCTATAAACTCTTAACACAAATAAATATAAGCTTTTAAGGACTTGTTCGGTGACGTTTTTATGTTCCTCATTCCGTTTAAAATAGAAAAAAGTGTTTTGAAAAAATGAAAAACAAGAACTAGTAACCAAATGATTATTAAGTACAGAGTTCATAAAATAGGAATAATATCGAACATACCCTAATTTTTTCACTATATATCGTTAATATATACGTGTTTCATGAATGTAGGATCGAGCGTTATAATCCACAATGCCAATTGACTTATCTACCAATTCCTTATCCCAAAGACAAATGCTTGGCAAGAGTGCTGAAAACTGCATGACAACGTATAGTGCTTCTCAATCTTATTTATCCCAACAAATGAACTTTATGATTGTATTCAGCTTTTGAAATATGGTATATGTTTGTGTGTATGTGTGTACTTATGTACTCATCAAAATTTAATAATTAATACTTTGTTATTACTATAATCATGAAAGAATTTAATTTTGTTCAAAACTTTTCAAGGTACAAACTAGATATTTTTGCTACATTTCC

mRNA sequence

ATTCAACACCAAGGGGCAATACAAAAGTGTGTTCTTAAGGAAAGGGATCCAAAGCAAAGGTGTTAGCTGCAGAAATCAATGGAGTTCATAAATGGGGTTTTCTTGATTCTCCCTGTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTCCCTCCTGGTGATCTCTGTTGGCCACTTCTTGGCTCTACTTTATCCTTCCTCAAATCTTTCACCGTTGGTCCACCCGAAAGCTTTATCCGTGCCTTTACCACCAGGTATGGGAAAGTTGACATGTACAAGACACACATTTTTGGGAGGGCAAGCATACTTGTCTGCAAGCCTGAAATCTGTCGCCAAGTACTAAGCGATGAAACCATATTTGTTCCTGGTTATCCCGCCAGCATGCAAACGTTATTTGGTAGAAATACGCTGCACCGAGCCTCAAAGGCGGAGCACCGGAAGCTCCGACGGGTGACAACGGCGCCGATCAGTAGCCACGCTGCGCTCGAGATGTACGTCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATAATGATATTTCACTTGGGTTCTTCTCTTTGCCCATCAACTTTCCTGGCTTCTACTTCCATAAATCTCTCAAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAATGAGAAGAGGTTGATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCACAAGATATGATGGATTTGATGATAGAAGTGGAAGATGAAGATGGAGAAGGGATGGACAATGAGACCATTGCAGATTTGATTTTTGGTAAGTTATTTGCAGGCCAGGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATCTGTTCCAAAAGGCCAAGGAAGAACAAGAAGATATCATTAGTCGAAGACCTTCAACACAAAAGGGTATAAATCTCGCTGAATTTAAACAAATGAAATTTCTTTCTCAGGTGATAGATGAGACGCTTCGTCTTAGTGGTATTATATTTGCTACTTTTCGTGAGGCAACAACTGACGTTAATATCAATGGTAAGCTTATACCAAAAGGATGGAAAGTCATACTTTGGCTTCGAGAGCTTTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCAAGATGGGATAATTTTATAGGCAACCCAGGTGCTTTTACCCCGTTCGGATTAGGAGTCAGGATGTGCCCTAGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCGAGCGTTATAATCCACAATGCCAATTGACTTATCTACCAATTCCTTATCCCAAAGACAAATGCTTGGCAAGAGTGCTGAAAACTGCATGACAACGTATAGTGCTTCTCAATCTTATTTATCCCAACAAATGAACTTTATGATTGTATTCAGCTTTTGAAATATGGTATATGTTTGTGTGTATGTGTGTACTTATGTACTCATCAAAATTTAATAATTAATACTTTGTTATTACTATAATCATGAAAGAATTTAATTTTGTTCAAAACTTTTCAAGGTACAAACTAGATATTTTTGCTACATTTCC

Coding sequence (CDS)

ATGGAGTTCATAAATGGGGTTTTCTTGATTCTCCCTGTGGGCTTTGTTTTAGTTTTTGAGGTTTTGAAGAGATTGAATGGTTTGTATTATGCTGTGAAGTTGGGGAAGAAATGGGGTGAGCTCCCTCCTGGTGATCTCTGTTGGCCACTTCTTGGCTCTACTTTATCCTTCCTCAAATCTTTCACCGTTGGTCCACCCGAAAGCTTTATCCGTGCCTTTACCACCAGGTATGGGAAAGTTGACATGTACAAGACACACATTTTTGGGAGGGCAAGCATACTTGTCTGCAAGCCTGAAATCTGTCGCCAAGTACTAAGCGATGAAACCATATTTGTTCCTGGTTATCCCGCCAGCATGCAAACGTTATTTGGTAGAAATACGCTGCACCGAGCCTCAAAGGCGGAGCACCGGAAGCTCCGACGGGTGACAACGGCGCCGATCAGTAGCCACGCTGCGCTCGAGATGTACGTCGATCATATTGAACACACAGTGATCAGTGGGTTGGAGGAATGGTCAAGTGTGGAGAAGCCATTGAAGCTGTTGACAGAGATAAAGGAGCTTACCTTCAAAATTATTTGGAACATTTTTATGGGTTCAACTTCCATAGACTATACCACAAAAGAAATGGAGGCTTTGTATAATGATATTTCACTTGGGTTCTTCTCTTTGCCCATCAACTTTCCTGGCTTCTACTTCCATAAATCTCTCAAGGCTCGAAAAAGATTGTATGAAATACTTCTATCTATTGTGAATGAGAAGAGGTTGATGAAGAAAAGCAAAGGGGAAAGTTGGGAAGCACAAGATATGATGGATTTGATGATAGAAGTGGAAGATGAAGATGGAGAAGGGATGGACAATGAGACCATTGCAGATTTGATTTTTGGTAAGTTATTTGCAGGCCAGGAGACTTCAGCTTTCACTACAATGTGGGCAATTATATTTCTTACAGATCATCCACATCTGTTCCAAAAGGCCAAGGAAGAACAAGAAGATATCATTAGTCGAAGACCTTCAACACAAAAGGGTATAAATCTCGCTGAATTTAAACAAATGAAATTTCTTTCTCAGGTGATAGATGAGACGCTTCGTCTTAGTGGTATTATATTTGCTACTTTTCGTGAGGCAACAACTGACGTTAATATCAATGGTAAGCTTATACCAAAAGGATGGAAAGTCATACTTTGGCTTCGAGAGCTTTACATGGATGAAGAGCTACATCCTTCCCCACAAGACTTCAATCCATCAAGATGGGATAATTTTATAGGCAACCCAGGTGCTTTTACCCCGTTCGGATTAGGAGTCAGGATGTGCCCTAGACGTGATCTTGCCAAGCTCGAGATCTCAATTTTCCTTCACCATTTTCTCCTCAATTACAAGATCGAGCGTTATAATCCACAATGCCAATTGACTTATCTACCAATTCCTTATCCCAAAGACAAATGCTTGGCAAGAGTGCTGAAAACTGCATGA

Protein sequence

MEFINGVFLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLKTA

Homology

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BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.6e-144
Identity = 244/480 (50.83%), Postives = 351/480 (73.12%), Query Frame = 0


Query: 11  LPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPESFI 70
           L V ++   + ++ LNG YY VKL +K   LPPGD+ WPL+G  LSF+K F+ G P+SFI
Sbjct: 13  LLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSFIKDFSSGHPDSFI 72

Query: 71  RAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNTLHR 130
                +YG+  +YKTH+FG  SI+VC+P++CR+VL+D+  F  GYP S++ L     +  
Sbjct: 73  NNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPKSIKELARCRPMID 132

Query: 131 ASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKELTFK 190
            S AEHR  RR+ T+PI  H AL MY++ +E  VI+ LEE SS++ P++LL E+K+++FK
Sbjct: 133 VSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHPVELLKEMKKVSFK 192

Query: 191 IIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILLSIV 250
            I ++FMGS++ D   K++ + + D+  G FS+PIN PGF FHK+L+ARK+L +I+  +V
Sbjct: 193 AIVHVFMGSSNQD-IIKKIGSSFTDLYNGMFSIPINVPGFTFHKALEARKKLAKIVQPVV 252

Query: 251 NEKRLM-KKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTM 310
           +E+RLM +    E  + +D++D+++EV+DE+G  +++E I+DL+ G LFAG E++A + M
Sbjct: 253 DERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGLLFAGHESTATSLM 312

Query: 311 WAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGIIF 370
           W+I +LT HPH+ +KAKEEQE+I   R S+QK ++L E KQM +LSQVIDETLR + I F
Sbjct: 313 WSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQVIDETLRCANIAF 372

Query: 371 ATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAFTP 430
           ATFREAT DVNING +IPKGW+V++W R ++MD E +P+P++FNPSRWD++    G F P
Sbjct: 373 ATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSRWDDYNAKAGTFLP 432

Query: 431 FGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLKTA 490
           FG G R+CP  DLAKLEISIFLH+FL NY++ER NP+C +T LP+  P D CLA+V+K +
Sbjct: 433 FGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSKPTDNCLAKVIKVS 491

[Close]

BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 446.0 bits (1146), Expect = 5.3e-124
Identity = 222/481 (46.15%), Postives = 321/481 (66.74%), Query Frame = 0


Query: 8   FLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPE 67
           F ++ +G  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNT 127
           SFI+++ TRYG+  +YK H+FG   +LV  PE CR+VL+D+  F  G+P S   L GR +
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKEL 187
               S  EH++LRR+T+AP++   AL +Y+  IE TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y +++ G  ++ IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFT 307
           SIV  +R  +K    S   +DM+D +I+V+DE+G  +D+E I DL+   L AG E+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGI 367
           TMWA I + +HP + QKAKEEQE I+ +R   QK + L E ++M +LSQVIDETLR+   
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 IFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAF 427
               FREA +DV ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLK 487
            PFGLG  +CP  DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 487

Query: 488 T 489
           T
Sbjct: 491 T 487

[Close]

BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 4.5e-123
Identity = 216/466 (46.35%), Postives = 308/466 (66.09%), Query Frame = 0


Query: 21  VLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPESFIRAFTTRYGKV 80
           +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+SF R    RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  DMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNTLHRASKAEHRKLR 140
            +YK H+FG  SI+V   + CR+VL+D+  F PG+P S   L GR +    S  EH++LR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGST 200
           R+T AP++ H AL  Y+ +IE  VI+ L++W+ + +  + LT +++LTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 SIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILLSIVNEKRLMKKSK 260
           S +     +E  Y  ++ G  ++ +N PGF +H++LKARK L     SIV E+R  +K  
Sbjct: 205 S-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 264

Query: 261 GESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFLTDHPH 320
             S   +DM+D ++ V+DEDG+ +D+E I D++   L AG E+S  T MWA +FL +HP 
Sbjct: 265 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 324

Query: 321 LFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGIIFATFREATTDVN 380
           + Q+AK EQE I+  RP  QKG++L E ++M+FLSQV+DETLR+       FREA TDV 
Sbjct: 325 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 384

Query: 381 INGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDN-FIGNPGAFTPFGLGVRMCPR 440
           +NG LIPKGWKV+ W R++++D E+ P P+ F+P+RWDN F+   GAF PFG G  +CP 
Sbjct: 385 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPG 444

Query: 441 RDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARV 486
            DLAKLEISIFLHHFLL Y+++R NP+C + YLP   P D CLAR+
Sbjct: 445 NDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 2.5e-121
Identity = 214/478 (44.77%), Postives = 315/478 (65.90%), Query Frame = 0


Query: 14  GFVLVFEVLKRLNGLYYAVKLG-KKWGELPPGDLCWPLLGSTLSFLKSFTVGPPESFIRA 73
           G VLV  V++R +       LG ++   LPPG++ WP++GS  +FL++F  G P++FI +
Sbjct: 27  GVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAFIAS 86

Query: 74  FTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNTLHRAS 133
           F  R+G+  +Y+T +F   +IL   PE C+QVL D+  FV G+P +  TL G  +    S
Sbjct: 87  FIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFVNMS 146

Query: 134 KAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEK-PLKLLTEIKELTFKI 193
             +HR++R++T API+   AL  Y+  I+ TV++ L  WSS E   ++ LTE++ +TFKI
Sbjct: 147 YDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMTFKI 206

Query: 194 IWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILLSIVN 253
           I  IFM S + D T + +E  Y D++ G  ++ IN PGF ++++L+AR++L  +L  +++
Sbjct: 207 IVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQGVLD 266

Query: 254 EKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTMWA 313
            +R       +   A DMMD +IE EDE G  + ++ I D++   L AG E+S   TMWA
Sbjct: 267 GRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHITMWA 326

Query: 314 IIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGIIFAT 373
            +FL ++P +F +AK EQE+I+   P+TQ G+ L +FK+M FLSQV+DETLR   I F +
Sbjct: 327 TVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNISFVS 386

Query: 374 FREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAFTPFG 433
           FR+AT D+ +NG LIPKGWKV LW R ++MD++++P P+ FNPSRW+      G F PFG
Sbjct: 387 FRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFLPFG 446

Query: 434 LGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLKTA 490
           LG R+CP  DLAKLEIS+FLHHFLL YK++R NP+C++ YLP P P D CLA + K +
Sbjct: 447 LGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKVS 503

[Close]

BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 5.7e-118
Identity = 210/480 (43.75%), Postives = 310/480 (64.58%), Query Frame = 0


Query: 9   LILPVGFVLVFEVLKRLNGLYYAVKLG-KKWGELPPGDLCWPLLGSTLSFLKSFTVGPPE 68
           L+  V  V+V    +R +G Y    LG  +   LPPG++ WPL+G   +FL++F  G P+
Sbjct: 39  LVGAVALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPD 98

Query: 69  SFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNT 128
           +FI +F  R+G+  +Y++ +F   ++LV   E C+QVL D+  FV G+P +   L G  +
Sbjct: 99  AFIASFVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRS 158

Query: 129 LHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKEL 188
                  EHR++R++T API+   AL  Y+  I+ TV S L  W+     ++ LTE++ +
Sbjct: 159 FVAMPYDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRM 218

Query: 189 TFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILL 248
           TFKII  IF+G      TT+ +E  Y +++ G  ++ IN PGF +  +L+AR+RL  +L 
Sbjct: 219 TFKIIVQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQ 278

Query: 249 SIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFT 308
            +++E+R   ++KG S    DMMD +IE +DE G  +D++ I D++   L AG E+S   
Sbjct: 279 GVLDERR-AARAKGVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHI 338

Query: 309 TMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGI 368
           TMWA +FL ++P +F +AK EQE I+   PS+Q+G+ L +F++M++LSQVIDETLRL  I
Sbjct: 339 TMWATVFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNI 398

Query: 369 IFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAF 428
            F +FR+AT DV +NG LIPKGWKV LW R ++MD +++P P  F+PSRW+      G F
Sbjct: 399 SFVSFRQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTF 458

Query: 429 TPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLK 488
             FGLG R+CP  DLAKLEIS+FLHHFLL YK+ R NP+C++ YLP P P D CLA++ +
Sbjct: 459 LAFGLGARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITR 516

[Close]

BLAST of CmaCh16G010780 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-125
Identity = 222/481 (46.15%), Postives = 321/481 (66.74%), Query Frame = 0


Query: 8   FLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPE 67
           F ++ +G  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNT 127
           SFI+++ TRYG+  +YK H+FG   +LV  PE CR+VL+D+  F  G+P S   L GR +
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKEL 187
               S  EH++LRR+T+AP++   AL +Y+  IE TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y +++ G  ++ IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFT 307
           SIV  +R  +K    S   +DM+D +I+V+DE+G  +D+E I DL+   L AG E+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGI 367
           TMWA I + +HP + QKAKEEQE I+ +R   QK + L E ++M +LSQVIDETLR+   
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 IFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAF 427
               FREA +DV ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLK 487
            PFGLG  +CP  DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 487

Query: 488 T 489
           T
Sbjct: 491 T 487

[Close]

BLAST of CmaCh16G010780 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 446.0 bits (1146), Expect = 3.8e-125
Identity = 222/481 (46.15%), Postives = 321/481 (66.74%), Query Frame = 0


Query: 8   FLILPVGFVLVFEVLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPE 67
           F ++ +G  ++  VLKR+N   Y  KLG+K   LPPGDL WP++G+  SFL++F    PE
Sbjct: 11  FPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDPE 70

Query: 68  SFIRAFTTRYGKVDMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNT 127
           SFI+++ TRYG+  +YK H+FG   +LV  PE CR+VL+D+  F  G+P S   L GR +
Sbjct: 71  SFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRKS 130

Query: 128 LHRASKAEHRKLRRVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKEL 187
               S  EH++LRR+T+AP++   AL +Y+  IE TV + LE+WS + + ++ L+ +++L
Sbjct: 131 FVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKMGE-IEFLSHLRKL 190

Query: 188 TFKIIWNIFMGSTSIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILL 247
           TFK+I  IF+ S S ++    +E  Y +++ G  ++ IN PGF +H++LKARK+L     
Sbjct: 191 TFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAFQ 250

Query: 248 SIVNEKRLMKKSKGESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFT 307
           SIV  +R  +K    S   +DM+D +I+V+DE+G  +D+E I DL+   L AG E+S   
Sbjct: 251 SIVTNRRNQRKQNISS-NRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGHL 310

Query: 308 TMWAIIFLTDHPHLFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGI 367
           TMWA I + +HP + QKAKEEQE I+ +R   QK + L E ++M +LSQVIDETLR+   
Sbjct: 311 TMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVITF 370

Query: 368 IFATFREATTDVNINGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDNFIGNPGAF 427
               FREA +DV ++G +IPKGWKV+ W R +++D E++P P+ F+PSRW+ +    G F
Sbjct: 371 SLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGTF 430

Query: 428 TPFGLGVRMCPRRDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARVLK 487
            PFGLG  +CP  DLAKLEISIFLHHFLL Y++ER NP C + +LP   PKD CLAR+ +
Sbjct: 431 LPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARITR 487

Query: 488 T 489
           T
Sbjct: 491 T 487

[Close]

BLAST of CmaCh16G010780 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 443.0 bits (1138), Expect = 3.2e-124
Identity = 216/466 (46.35%), Postives = 308/466 (66.09%), Query Frame = 0


Query: 21  VLKRLNGLYYAVKLGKKWGELPPGDLCWPLLGSTLSFLKSFTVGPPESFIRAFTTRYGKV 80
           +++++N   Y   LG+    LPPGDL WP +G+ LSFL++F    P+SF R    RYG  
Sbjct: 25  LVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDPDSFTRTLIKRYGPK 84

Query: 81  DMYKTHIFGRASILVCKPEICRQVLSDETIFVPGYPASMQTLFGRNTLHRASKAEHRKLR 140
            +YK H+FG  SI+V   + CR+VL+D+  F PG+P S   L GR +    S  EH++LR
Sbjct: 85  GIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRKSFVGISFEEHKRLR 144

Query: 141 RVTTAPISSHAALEMYVDHIEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGST 200
           R+T AP++ H AL  Y+ +IE  VI+ L++W+ + +  + LT +++LTF+II  IF+ S 
Sbjct: 145 RLTAAPVNGHEALSTYIPYIEENVITVLDKWTKMGE-FEFLTHLRKLTFRIIMYIFLSSE 204

Query: 201 SIDYTTKEMEALYNDISLGFFSLPINFPGFYFHKSLKARKRLYEILLSIVNEKRLMKKSK 260
           S +     +E  Y  ++ G  ++ +N PGF +H++LKARK L     SIV E+R  +K  
Sbjct: 205 S-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAFQSIVTERRNQRKQN 264

Query: 261 GESWEAQDMMDLMIEVEDEDGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFLTDHPH 320
             S   +DM+D ++ V+DEDG+ +D+E I D++   L AG E+S  T MWA +FL +HP 
Sbjct: 265 ILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGHTIMWATVFLQEHPE 324

Query: 321 LFQKAKEEQEDIISRRPSTQKGINLAEFKQMKFLSQVIDETLRLSGIIFATFREATTDVN 380
           + Q+AK EQE I+  RP  QKG++L E ++M+FLSQV+DETLR+       FREA TDV 
Sbjct: 325 VLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVITFSLTAFREAKTDVE 384

Query: 381 INGKLIPKGWKVILWLRELYMDEELHPSPQDFNPSRWDN-FIGNPGAFTPFGLGVRMCPR 440
           +NG LIPKGWKV+ W R++++D E+ P P+ F+P+RWDN F+   GAF PFG G  +CP 
Sbjct: 385 MNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAGAFLPFGAGSHLCPG 444

Query: 441 RDLAKLEISIFLHHFLLNYKIERYNPQCQLTYLPIPYPKDKCLARV 486
            DLAKLEISIFLHHFLL Y+++R NP+C + YLP   P D CLAR+
Sbjct: 445 NDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of CmaCh16G010780 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-66
Identity = 148/446 (33.18%), Postives = 226/446 (50.67%), Query Frame = 0


Query: 41  LPPGDLCWPLLGSTLSFLKSFTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEI 100
           LPPG +  P +G TL          P SF      +YG  D++KTHI G   +++  PE 
Sbjct: 49  LPPGSMGLPYIGETLRLYTE----NPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 101 CRQVL-SDETIFVPGYPASMQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDH 160
            R VL S   +F P YP S + + G   L       H  L+R+  +     +AL   V H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTVSH 168

Query: 161 IEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLG 220
           IE  V+  L  W+S +K +  L  +K   F +      G      T   ++ LY  +  G
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 221 FFSLPINFPGFYFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDE 280
           + S+P++ PG  FHKS+KAR  L E L  ++ ++R   + +G       ++ +++  +D+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 281 DGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPST 340
              G+ +  IAD I G +FA  +T+A    W + +L DHP+L Q+   EQ  I  +    
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 348

Query: 341 QKGINLAEFKQMKFLSQVIDETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLREL 400
            + I+  + ++M   ++VI ETLR + ++  TFREA  DV  +G LIPKGWKV+   R +
Sbjct: 349 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 408

Query: 401 YMDEELHPSPQDFNPSRWDNFIGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYK 460
           +   E  P P+ F+PSR++     P  + PFG GV  CP  +LAKLE+ I LHH   +++
Sbjct: 409 HHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 468

Query: 461 IERYNPQCQLTYLPIPYPKDKCLARV 486
            E    +  + Y P P PK     RV
Sbjct: 469 WEVIGDEEGIQYGPFPVPKKGLPIRV 479

[Close]

BLAST of CmaCh16G010780 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-66
Identity = 148/446 (33.18%), Postives = 226/446 (50.67%), Query Frame = 0


Query: 41  LPPGDLCWPLLGSTLSFLKSFTVGPPESFIRAFTTRYGKVDMYKTHIFGRASILVCKPEI 100
           LPPG +  P +G TL          P SF      +YG  D++KTHI G   +++  PE 
Sbjct: 49  LPPGSMGLPYIGETLRLYTE----NPNSFFATRQNKYG--DIFKTHILGCPCVMISSPEA 108

Query: 101 CRQVL-SDETIFVPGYPASMQTLFGRNTLHRASKAEHRKLRRVTTAPISSHAALEMYVDH 160
            R VL S   +F P YP S + + G   L       H  L+R+  +     +AL   V H
Sbjct: 109 ARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFMP-SALRPTVSH 168

Query: 161 IEHTVISGLEEWSSVEKPLKLLTEIKELTFKIIWNIFMGSTSIDYTTKEMEALYNDISLG 220
           IE  V+  L  W+S +K +  L  +K   F +      G      T   ++ LY  +  G
Sbjct: 169 IELLVLQTLSSWTS-QKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERG 228

Query: 221 FFSLPINFPGFYFHKSLKARKRLYEILLSIVNEKRLMKKSKGESWEAQDMMDLMIEVEDE 280
           + S+P++ PG  FHKS+KAR  L E L  ++ ++R   + +G       ++ +++  +D+
Sbjct: 229 YNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAKDQ 288

Query: 281 DGEGMDNETIADLIFGKLFAGQETSAFTTMWAIIFLTDHPHLFQKAKEEQEDIISRRPST 340
              G+ +  IAD I G +FA  +T+A    W + +L DHP+L Q+   EQ  I  +    
Sbjct: 289 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 348

Query: 341 QKGINLAEFKQMKFLSQVIDETLRLSGIIFATFREATTDVNINGKLIPKGWKVILWLREL 400
            + I+  + ++M   ++VI ETLR + ++  TFREA  DV  +G LIPKGWKV+   R +
Sbjct: 349 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRI 408

Query: 401 YMDEELHPSPQDFNPSRWDNFIGNPGAFTPFGLGVRMCPRRDLAKLEISIFLHHFLLNYK 460
           +   E  P P+ F+PSR++     P  + PFG GV  CP  +LAKLE+ I LHH   +++
Sbjct: 409 HHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTSFR 468

Query: 461 IERYNPQCQLTYLPIPYPKDKCLARV 486
            E    +  + Y P P PK     RV
Sbjct: 469 WEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:

BLAST of CmaCh16G010780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.6e-144	50.83	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
Q9C5Y2	5.3e-124	46.15	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
O23051	4.5e-123	46.35	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q5VRM7	2.5e-121	44.77	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	5.7e-118	43.75	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]

BLAST of CmaCh16G010780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32440.1	3.8e-125	46.15	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	3.8e-125	46.15	ent-kaurenoic acid hydroxylase 2	[more]
AT1G05160.1	3.2e-124	46.35	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G29090.1	4.0e-66	33.18	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	4.0e-66	33.18	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 356..367 score: 40.43 coord: 428..437 score: 54.97 coord: 299..316 score: 32.77 coord: 437..448 score: 31.83
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 42..462 e-value: 9.1E-65 score: 219.2
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 395..419 score: 25.74 coord: 437..460 score: 26.46 coord: 427..437 score: 53.46 coord: 288..305 score: 25.55 coord: 308..334 score: 24.84
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 34..487 e-value: 6.8E-102 score: 343.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 40..485
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 19..466
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 19..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010780.1	CmaCh16G010780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen