CmaCh16G010720 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010720
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	beta-amyrin 11-oxidase-like isoform X1
Location	Cma_Chr16: 8215647 .. 8222181 (-)
RNA-Seq Expression	CmaCh16G010720
Synteny	CmaCh16G010720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTAGCAATGGAGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGACTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGCCTAAGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAATTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGGTCATTTCTTCATCCATTTCATTCAAGTTTATGATTAATATGTTCAAATAATATGTTTCTTCCCCTCATTCTACTTAGAGTGTGCATCGAAATTGATATTTGAAACCGGCGTAATCGTATTGTTGGAGTCTTATATTGGCTTATTAAGGGGAACATCATGGGTATATAAGTAGGAAACACTATATATGAGACCAAATCAAAAGCAAACTCATGAAACTTTATACTTTAAGTGAACAATATCGTACCATTGTGTAGGTTCGTTGTCACTAACAAGTGGTATCAAAGTCATGTTCTTAATAGACATGTCAATAGAATTTTCAGGTGTCGAACAAATTAAAGATTTATGGTAGAGCTCGATTAAACTAGCAAAAAAAAAAAAAAAAAGAGTTGTTGTGCAGGATAGTGCTAGATGAATTGATGACTATTTAAAAAGAGCTTCAATGATACTTTGTTCTTGAGGAAGATTGTAGGAGTGGTTAATTGAAACCCTTACGTGGAAACCAAAACCAAAGTCAAGAAAGTTTATGCTTAAAGTGGACAATTTCATTCCATTTATGGAGGTTCGTTGTTACTAACAGGTGTTTGGATCCTAATTTTCATTCCAAGTCACACATCGTTTTCAAACCTTCTAACGAATTACTACTCCTAAGTTCAAGAGTATTTCGTATAATTTAGCTTTCATTTGACACATAATTGCTACTTTAGAAACTACTCTCGGAAATGAAGAGGCGATATTAGCTAATTGTCGATCAAAAGAATAGAGTTCGATCAGAGCTCGAACGACAAGAATCGAGATACTATTTTGATATCTCTCATCAAAACTTTCTTTTTTTTTTTTTTTACAGATATGGGAAAAGAGATATGTACAAAACACACCTACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTCTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGCTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGTAAGCCCCTTCTTTTCTTTCCTTTTTTACTTTGTTTCTTGTCAATAATTCATGTGGGGTTTGGATAAGTAGTTGTATTTAATCATCGAAACGGTTAAAAAGACTAAATTGACCGTAATTATGTTGAGCATTGTTGGGAGAGTAGTCCAAATAAAGAATACATCTCTGTTAGTACGGGGGCATTTTGAAAAAATCAAAAGTAAAGTCACGAGAGTTTATTCTTAAAGTGTATCAAAGTCATGTCCTTGACTTAGCTCCGAAAGGAGGTGGATTGTGAGATCCCACATTGGTTGGAGATGAGAACGAAACATTTTTTTAAAAGAGTTAGGAAAACTCTCCCTAGCAAACGTGTTCTAAAAACTTTGAGGAAAACCCGAAAGGAGCGATGGGCTTGAGCTGTTATAGTGGGCCTCAAAGGTGTAGTCAAAAGTGACTCAAGTGTTGAACAAAATGTGTATTTTATTCAAGAGCTTCAGAGGAGTCGAGCCTCGAAGGTTGTTCGAGGGCTACATAAGCCTCAGAGAAGGCTCTATGGTGTGAGAACCCACATCCGTTGGAGAGGAGAATGAAACATTGTTTATAAGGGTGTGAGAACTTCTCCCTAGTAGATGTGTTTTAAAAACCTAAAAGTTCGAAAAGAAAAGTCCAAAGAGGACAATATCTGCTACCAGTGGGCTTGAGTTGTTACATATGGTGTACTTTGTCGGGAGAGGAGTCCAAGTAAAGAATACATCTCCATTGGTATGAACCTTCTTGAAAAAATCGAAAGTAAAATCATAAGAGGGTCGTTATTAATAACAACTTATCCACGAGTTTACGTAGCAATAATTTAACATAGTATTATACAAAGTTCTATGTTTGAATCCTTATAATGTCATGGTATCTCTAGTTCATTTTAGATTAAAATATACAAGATAACACTTAGTACATTACCCAAAAAATAAACAAAGAATACTAGTTTGAGTAAAAAATAGAGCTCTATTCGTACGAGACATTTTGGGAATATCAAAAGCAAGACCTTTTGAGAATATCAAAAGCAAAGTCATAAGAGTTTATACTCAAAGTGGACAATATCATATTATTGTAGAGGATCATGATTACTAACCTCGTATTAGAGTCATGTCTTTAACTTAGTCATGTCAATAGAATTCTCAAGTTTCGAACAAATAACTTATGAGCCTCGAACATATAGTCAAAAATGACTCAAGTGTCGAACAAATGATGTACTTTATTTGAGAGCTCTAGAGAAAGAGTCAAACCTCGATTAAGGGAAGGTTGTTCGAGGGTTTCATAGGCCTCAGAGGAGACTCTATGGTGTACTTTGTTCAAGGAGAGAGTTATTGAGGATTGTTGGGAGATGAGTCCAAGTAAAGAATACATCTCCATTGATATTAGCCCTTTTGGGAAACCAAAAGTAAAGGCATGAGAGCTTATGTTCAAAGTAGACAATATCATACCATTATTGTGAGATCCCACATCAGTTCGGGAGGAGAACGAAACAAATTTTTTAAGAGAAGCTCAAAAGGAAAAATCTTAAAGAAGACAATATCTATTAGGGGTAGGCTTTGGTCATTACAATTAGAGGGTCGTGATTACTAACAACTTATCAACAAGTTTACACTGTCCTAATTTAACACAGTATCAAATACAAGGTCGTGTTTGAATCCTTAGCATGTCATCGTCCTCTCTAGTTCATTGTAGATTAAAAAATGTGTTCATTTACTAATACTTGAGAATGACAAATACTTATTGTATTTATGTCACTATCTTACCGCTAAGAGATTTAAACTATAATATTTGAAACTAATTCATCATTGTTAGGTTGGACTTATTATATGCTTTCTCTATCCCATTTGATCAATGCAAAATAATACTTTTATTATTGTAATTAAATATATTTTGCAGTTAATATCTTGATTATGCCAAATTCAAACGTGTGACGGGTCAAAATTAGGTTAAATTAATAAAAATACTCTAGAATTTTGCACTTTGTCTAAACCTTAAACTTTCAAAAAGTTCAAAAACACCTTAATATTATGTTTCAAAAAAGTATCTTCCTTCTAGATATTTTTAAACATGTTAGCAACGGGAGATATTAATGAAATTTTTAAAAAATCAAGGATATTTTTTAACGTACTAATAGTTTCCATCCAAACTGAGGGCGGGTACTTTTTTTTTTAGACTCGTTCAAAAGTTTTTAGAGTAATTTTGAAAAGTTTTAGGGTATTTTTGAAATAAAAGTAAACGGTTTATAGGTACTTGTATTAATTTAGCATCAAAACTATTCAAAGTCAGTGTTATACCATTCCATATCAACGTCATACTCACTCCAAGTCTCAATTTTTATCGAGGACAATATTGTAACTTTTGAAAGAACGTAAGCATGTTTAAAACAAATAGCAAACTTTAGTGGTATTTTATTTTTATTTTTTTTCTCTATTAACAAATTTAAACATAGGCTAGAAAGAAGTTGCTCGACATACTTCAATCCATCATCGACCAAAAGAGATTGGTGAGGAAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCAGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGTTAGTGATTATTTCTTTCTTAACATAGCTACATTTCATGTTCTATTCAACTATATAATATTTGGGATTTGTAGTATTCATTATATGATCTATTATATTATAAAATCTCTATGTTTTAGGAAGAGCAAGAACTTATCGTTGCACAAAGACCCACAACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACGGATGAAATTTCTTTCTCAGGTATGATTTTAATATTTATTTTTCTTGAGATTGAAATTACCCATTTATATTGTGAGATCTCACATCGGTTGTAGAGGAGAATGAAGCCTTCCTTAAAAGAGTGTGGAAACCTCTCCCTAGTATACAAGTTTTAAAACAATGAGGTTGATGGCAATATGTAATGGGCCAAAGCGAATAATATCTACTAGCGGTGGACTTGAGCTGTTACAAATGGTATCAGAATTAGACACCTTGTGCTAGTGAGGACGCTGGCTCCCAAGAGGGGTGGATTGTGAGATCCCACATCGATTGAAAAGGGGAACAAAGCATTCCTCATAAGAGTGTGGAAACCTCTCTTGTAGATTGTGAGATTTCATATCGGTTGGAGAGGGGAACGAAACATTCCTTATAAGAGTGTGGAAATCTCTCCTGTAGATTGTGAGATTCCACCTCGGTTGGAGAGGGGAACGAAGCATTCCTTATAAGAGTGTGGAAACCTCTCCTGTAGATTGTGAGATTCCACCTCGGTTGGAGAGGGGAACGTTACATAACATACCCAAACGAACATTAGAAAATCAAAATTAAGAATACATTTTTTGATATTTCAGATTATAGATGAGACGTTACGTGTTGGTGGTGTCACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGTGATTAAATTTCTATACATATATGTATGTTTGTCTTTCTAACATATGAAATATGATTTCTTGTACTTTATTTAGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACGAGAGTTCAACCCTTCAAGATGGGATGTATGTAACTTATTTATATCATTGCCAGCCTTTTTTATTTTTGTAAAGGATGTTTCGTTCCTCGTTTTCACAATCTTATAAGAGATGTTTCGTGCTCTATTCCAAACCCTTATAAGAGATTTTTTATGCCCCGTTTCCACACCCTTATAAGACATTCTTCGTGCCCCGTTTTCACACCCTTATAAGGGATGCTTATTGCCCCGTTTCCATACCCTTACAAAGAATGTTTCGTGCTTAGTTCTCCGTTTTGATCCTTGTTTTCACACCCTTAGAAGGAAGACTTTGTTTCCTTCTTTAACCAATGTGGGATCTCAAAGAGGCTTTCACACCTTTATACGTAATTCTCGTTTCCACACCTTTAAAAGGAATGTTTCATTTCATTCTCTAACCAATATGGGATCTCAAGAAGGTATTCACGCCTTTATAAGGAATCCTCGTTTCCACACCTTTAGAAGAAATGTTTTATTTCCTTCTCTAACCATTGTAGGATCTCAGAGAGGTTTTTACACCTTCATTTTTCTCTCTAAACGACGTGAGATCTCACATTTTTTTCTCTTTTGATCTAATTAATGGCATATTGAATGGAATTTTTCTTGCTTTGTAAAGAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGTAAGTTTACTCTTAACTTCTCATTAATTAATTCTATTAACTTCAACACAAAACATAAACAAAAACCTTTTCTTAATTACTACACAATCATTTTGTAATAATGCAGGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCTAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAATGAATACATATATATAAGTGTGTATGTATACATAGAACCTGATTCAACTATATTATACTTATGACTCTACTTTTTAAAGTACAGTTTAGTTCGTTATTATATGAATAGTATGAATTTTAATTGCATAACAAAAGGAATCGAGTATAGAGGTTGAACTCTGACTAGCAAGCACAATCAATCGCCCGAGATGTTGTTGTCTTTACTTGAAAAAATATTGAAAAATGTTAAGTATCATAAATACTCCGTGATTCCCCTATTGGAG

mRNA sequence

ATGTTAGCAATGGAGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGACTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGCCTAAGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAATTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGATATGGGAAAAGAGATATGTACAAAACACACCTACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTCTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGCTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGCTAGAAAGAAGTTGCTCGACATACTTCAATCCATCATCGACCAAAAGAGATTGGTGAGGAAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCAGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGAAGAGCAAGAACTTATCGTTGCACAAAGACCCACAACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACGGATGAAATTTCTTTCTCAGATTATAGATGAGACGTTACGTGTTGGTGGTGTCACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACGAGAGTTCAACCCTTCAAGATGGGATAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCTAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAATGAATACATATATATAAGTGTGTATGTATACATAGAACCTGATTCAACTATATTATACTTATGACTCTACTTTTTAAAGTACAGTTTAGTTCGTTATTATATGAATAGTATGAATTTTAATTGCATAACAAAAGGAATCGAGTATAGAGGTTGAACTCTGACTAGCAAGCACAATCAATCGCCCGAGATGTTGTTGTCTTTACTTGAAAAAATATTGAAAAATGTTAAGTATCATAAATACTCCGTGATTCCCCTATTGGAG

Coding sequence (CDS)

ATGTTAGCAATGGAGGTGAAGAACTGGTTGGTGTTGATTGTTGCAGCCTTTGTGTTTGTTTTGGGGATTTTGAAGAGACTAAATGGTTGGTATTATGCGGCCAAGCTTGGTAAAACATGGCCTAAGCTTCCTCCTGGTGATATGGGTTGGCCTCTTCTTGGTTCTACCATATCATACATCAAGAATTTCATCTCTGGTCAGCCAAGTAATTTCATTGCCAACTTTTACATAAGATATGGGAAAAGAGATATGTACAAAACACACCTACTTGGTAGAGCAACTATAATTGTTTGTTCATCTGAACTTTGTCGTCAAGTCTTAGGCGATGAAGACAACTTCAAACCTAGTTTACCCAAGAACATCAATATGCTAACCGGTAGAAAGTCACTGGTACAAGTCTTCAAGGCGGAGCACAAGCGGCTTCGGCGGTTGACGATGGCGCCGATCAGCGGCCACGCCGCGCTGGAGATGTACATCAATCATATTGAACACACAGTGATGAGTGGGTTAGAGGAATGGGCAAGCATGAAGAACCCAATTGAGCTATTGTCAGAGATAAAGAAGCTGACCTTCAAAATTATTTGGAACATTTTCATGGGTTCTTCTTCTTTAGAGTCTTCCATTGGAGAAATGGAGGGTTTGTTTTCTGAAGTTGCTTTAGGGTTCATATCATTGCCCATCAACTTTCCTGGCTTCAACCTCCATAAAGCACTCAAGGCTAGAAAGAAGTTGCTCGACATACTTCAATCCATCATCGACCAAAAGAGATTGGTGAGGAAAAGGCAAGGAACAAGTTGGGAAGCTAAAGACATGATGGATTTGCTTATGGAAGTGAAAGATGAAGATGGTGAAGAGTTGGATGATGAAACCATTAGAGATTTGATATTTGGTAAGCTATTTGCAGGCCATGAAACTTCAGCTTATACAGCAATGTGGGCAGTTTTGTTTCTTACAAAACATCCACATATTTTCCAAAGAGCCAAGGAAGAGCAAGAACTTATCGTTGCACAAAGACCCACAACTCAAAAGGGTATAATTCTCTCTGAGGTTAAACGGATGAAATTTCTTTCTCAGATTATAGATGAGACGTTACGTGTTGGTGGTGTCACATTAATGCTTTTTCGTGAAGCAGTAGTTGATGTCGAAATTAATGGTAAGATTATACCAAAGGGATGGAAAGTCATACCTTGGTTTGGGGAGCTTTATATGGATGAAAAGCTATTTCCTTCACCACGAGAGTTCAACCCTTCAAGATGGGATAATTATGTAGCCACGCCAGGAGCTTTCATCCCTTTTGGATCAGGAAGCAGGTATTGCCCCGGAAGTGATCTTGCCAAGCTCGAAATCTCAATTTTTCTTCATTATTTTCTCCTCAATTACAAGATTGAACAGCTTAATCCAAAATGTCCGATGACTTACATGCCATTTCCTCATCCTAAAGACAAATGCTTGGCAAGAGTGCATAAAGTTTAA

Protein sequence

MLAMEVKNWLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV

Homology

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BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot
Match: B5BSX1 (Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.1e-145
Identity = 248/492 (50.41%), Postives = 350/492 (71.14%), Query Frame = 0


Query: 4   MEVKNWLVL----IVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISY 63
           MEV +W+ +    ++  ++F    ++ LNGWYY  KL +    LPPGDMGWPL+G  +S+
Sbjct: 1   MEV-HWVCMSAATLLVCYIFGSKFVRNLNGWYYDVKLRRKEHPLPPGDMGWPLIGDLLSF 60

Query: 64  IKNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPK 123
           IK+F SG P +FI N  ++YG+  +YKTHL G  +IIVC  ++CR+VL D+ NFK   PK
Sbjct: 61  IKDFSSGHPDSFINNLVLKYGRSGIYKTHLFGNPSIIVCEPQMCRRVLTDDVNFKLGYPK 120

Query: 124 NINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNP 183
           +I  L   + ++ V  AEH+  RRL  +PI GH AL MY+  +E  V++ LEE +SMK+P
Sbjct: 121 SIKELARCRPMIDVSNAEHRLFRRLITSPIVGHKALAMYLERLEEIVINSLEELSSMKHP 180

Query: 184 IELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALK 243
           +ELL E+KK++FK I ++FMGSS+ +  I ++   F+++  G  S+PIN PGF  HKAL+
Sbjct: 181 VELLKEMKKVSFKAIVHVFMGSSN-QDIIKKIGSSFTDLYNGMFSIPINVPGFTFHKALE 240

Query: 244 ARKKLLDILQSIIDQKRL-VRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGK 303
           ARKKL  I+Q ++D++RL +        + KD++D+L+EVKDE+G +L+DE I DL+ G 
Sbjct: 241 ARKKLAKIVQPVVDERRLMIENGPQEGSQRKDLIDILLEVKDENGRKLEDEDISDLLIGL 300

Query: 304 LFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQ 363
           LFAGHE++A + MW++ +LT+HPHI ++AKEEQE I   R ++QK + L E+K+M +LSQ
Sbjct: 301 LFAGHESTATSLMWSITYLTQHPHILKKAKEEQEEITRTRFSSQKQLSLKEIKQMVYLSQ 360

Query: 364 IIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSR 423
           +IDETLR   +    FREA  DV ING IIPKGW+V+ W   ++MD + +P+P EFNPSR
Sbjct: 361 VIDETLRCANIAFATFREATADVNINGYIIPKGWRVLIWARAIHMDSEYYPNPEEFNPSR 420

Query: 424 WDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPH 483
           WD+Y A  G F+PFG+GSR CPG+DLAKLEISIFLHYFL NY++E++NP+C +T +P   
Sbjct: 421 WDDYNAKAGTFLPFGAGSRLCPGADLAKLEISIFLHYFLRNYRLERINPECHVTSLPVSK 480

Query: 484 PKDKCLARVHKV 491
           P D CLA+V KV
Sbjct: 481 PTDNCLAKVIKV 490

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BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot
Match: O23051 (Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 3.7e-133
Identity = 232/480 (48.33%), Postives = 331/480 (68.96%), Query Frame = 0


Query: 9   WLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQP 68
           W  L+V     +  +++++N W Y + LG+    LPPGD+GWP +G+ +S+++ F +  P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 69  SNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +F      RYG + +YK H+ G  +IIV +S+ CR+VL D+D FKP  P +   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKK 188
           S V +   EHKRLRRLT AP++GH AL  YI +IE  V++ L++W  M    E L+ ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRK 190

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDIL 248
           LTF+II  IF+ S S E+ +  +E  ++ +  G  ++ +N PGF  H+ALKARK L+   
Sbjct: 191 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 250

Query: 249 QSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+ ++R  RK+   S   KDM+D L+ VKDEDG+ LDDE I D++   L AGHE+S +
Sbjct: 251 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGH 310

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGG 368
           T MWA +FL +HP + QRAK EQE+I+  RP  QKG+ L E ++M+FLSQ++DETLRV  
Sbjct: 311 TIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVIT 370

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDN-YVATPG 428
            +L  FREA  DVE+NG +IPKGWKV+ WF ++++D ++FP PR+F+P+RWDN +V   G
Sbjct: 371 FSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAG 430

Query: 429 AFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           AF+PFG+GS  CPG+DLAKLEISIFLH+FLL Y++++ NP+CP+ Y+P   P D CLAR+
Sbjct: 431 AFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

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BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot
Match: Q9C5Y2 (Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2)

HSP 1 Score: 464.9 bits (1195), Expect = 1.1e-129
Identity = 233/479 (48.64%), Postives = 322/479 (67.22%), Query Frame = 0


Query: 9   WLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQP 68
           W  LI+     +  +LKR+N W Y +KLG+    LPPGD+GWP++G+  S+++ F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  RK Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQRK-QNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGG 368
             MWA + + +HP I Q+AKEEQE IV +R   QK + L E + M +LSQ+IDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

[Close]

BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot
Match: Q5VRM7 (Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 3.9e-119
Identity = 209/480 (43.54%), Postives = 319/480 (66.46%), Query Frame = 0


Query: 13  IVAAFVFVLGILKRLNGWYYAAKLG-KTWPKLPPGDMGWPLLGSTISYIKNFISGQPSNF 72
           +VA  V V  +++R + W   A LG +   +LPPG+MGWP++GS  ++++ F SG P  F
Sbjct: 24  VVAGVVLVDAVVRRAHDWVRVAALGAERRSRLPPGEMGWPMVGSMWAFLRAFKSGNPDAF 83

Query: 73  IANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLV 132
           IA+F  R+G+  +Y+T +    TI+  + E C+QVL D++ F    PK    L G KS V
Sbjct: 84  IASFIRRFGRTGVYRTFMFSSPTILAVTPEACKQVLMDDEGFVTGWPKATVTLIGPKSFV 143

Query: 133 QVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKN-PIELLSEIKKLT 192
            +   +H+R+R+LT API+G  AL  Y++ I+ TV++ L  W+S ++  +E L+E++++T
Sbjct: 144 NMSYDDHRRIRKLTAAPINGFDALTTYLSFIDQTVVASLRRWSSPESGQVEFLTELRRMT 203

Query: 193 FKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDILQS 252
           FKII  IFM S + ++++  +E  ++++  G  ++ IN PGF  ++AL+AR+KL+ +LQ 
Sbjct: 204 FKIIVQIFM-SGADDATMEALERSYTDLNYGMRAMAINLPGFAYYRALRARRKLVSVLQG 263

Query: 253 IIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTA 312
           ++D +R    +      A DMMD L+E +DE G  L D+ I D++   L AGHE+S +  
Sbjct: 264 VLDGRRAAAAKGFKRSGAMDMMDRLIEAEDERGRRLADDEIVDVLIMYLNAGHESSGHIT 323

Query: 313 MWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGGVT 372
           MWA +FL ++P IF RAK EQE I+   P TQ G+ L + K+M FLSQ++DETLR   ++
Sbjct: 324 MWATVFLQENPDIFARAKAEQEEIMRSIPATQNGLTLRDFKKMHFLSQVVDETLRCVNIS 383

Query: 373 LMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGAFI 432
            + FR+A  D+ +NG +IPKGWKV  W+  ++MD++++P P+ FNPSRW+      G F+
Sbjct: 384 FVSFRQATRDIFVNGYLIPKGWKVQLWYRSVHMDDQVYPDPKMFNPSRWEGPPPKAGTFL 443

Query: 433 PFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 491
           PFG G+R CPG+DLAKLEIS+FLH+FLL YK+++ NPKC + Y+P P P D CLA + KV
Sbjct: 444 PFGLGARLCPGNDLAKLEISVFLHHFLLGYKLKRANPKCRVRYLPHPRPVDNCLATITKV 502

[Close]

BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot
Match: Q43246 (Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 2.0e-118
Identity = 207/476 (43.49%), Postives = 317/476 (66.60%), Query Frame = 0


Query: 16  AFVFVLGILKRLNGWYYAAKLGKT-WPKLPPGDMGWPLLGSTISYIKNFISGQPSNFIAN 75
           A V V    +R +GWY  A LG     +LPPG+MGWPL+G   ++++ F SG+P  FIA+
Sbjct: 44  ALVVVDAAARRAHGWYREAALGAARRARLPPGEMGWPLVGGMWAFLRAFKSGKPDAFIAS 103

Query: 76  FYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRKSLVQVF 135
           F  R+G+  +Y++ +    T++V ++E C+QVL D+D F    PK    L G +S V + 
Sbjct: 104 FVRRFGRTGVYRSFMFSSPTVLVTTAEGCKQVLMDDDAFVTGWPKATVALVGPRSFVAMP 163

Query: 136 KAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKKLTFKII 195
             EH+R+R+LT API+G  AL  Y+  I+ TV S L  WA     +E L+E++++TFKII
Sbjct: 164 YDEHRRIRKLTAAPINGFDALTGYLPFIDRTVTSSLRAWADHGGSVEFLTELRRMTFKII 223

Query: 196 WNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDILQSIIDQ 255
             IF+G +  +++   +E  ++E+  G  ++ IN PGF    AL+AR++L+ +LQ ++D+
Sbjct: 224 VQIFLGGAD-QATTRALERSYTELNYGMRAMAINLPGFAYRGALRARRRLVAVLQGVLDE 283

Query: 256 KRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAV 315
           +R  R + G S    DMMD L+E +DE G  LDD+ I D++   L AGHE+S +  MWA 
Sbjct: 284 RRAARAK-GVSGGGVDMMDRLIEAQDERGRHLDDDEIIDVLVMYLNAGHESSGHITMWAT 343

Query: 316 LFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGGVTLMLF 375
           +FL ++P +F RAK EQE I+   P++Q+G+ L + ++M++LSQ+IDETLR+  ++ + F
Sbjct: 344 VFLQENPDMFARAKAEQEAIMRSIPSSQRGLTLRDFRKMEYLSQVIDETLRLVNISFVSF 403

Query: 376 REAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGAFIPFGS 435
           R+A  DV +NG +IPKGWKV  W+  ++MD +++P P +F+PSRW+ +    G F+ FG 
Sbjct: 404 RQATRDVFVNGYLIPKGWKVQLWYRSVHMDPQVYPDPTKFDPSRWEGHSPRAGTFLAFGL 463

Query: 436 GSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARVHKV 491
           G+R CPG+DLAKLEIS+FLH+FLL YK+ + NP+C + Y+P P P D CLA++ +V
Sbjct: 464 GARLCPGNDLAKLEISVFLHHFLLGYKLARTNPRCRVRYLPHPRPVDNCLAKITRV 517

[Close]

BLAST of CmaCh16G010720 vs. TAIR 10
Match: AT1G05160.1 (cytochrome P450, family 88, subfamily A, polypeptide 3 )

HSP 1 Score: 476.5 bits (1225), Expect = 2.6e-134
Identity = 232/480 (48.33%), Postives = 331/480 (68.96%), Query Frame = 0


Query: 9   WLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQP 68
           W  L+V     +  +++++N W Y + LG+    LPPGD+GWP +G+ +S+++ F +  P
Sbjct: 11  WFPLMVLGCFGLNWLVRKVNVWLYESSLGENRHYLPPGDLGWPFIGNMLSFLRAFKTSDP 70

Query: 69  SNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +F      RYG + +YK H+ G  +IIV +S+ CR+VL D+D FKP  P +   L GRK
Sbjct: 71  DSFTRTLIKRYGPKGIYKAHMFGNPSIIVTTSDTCRRVLTDDDAFKPGWPTSTMELIGRK 130

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKK 188
           S V +   EHKRLRRLT AP++GH AL  YI +IE  V++ L++W  M    E L+ ++K
Sbjct: 131 SFVGISFEEHKRLRRLTAAPVNGHEALSTYIPYIEENVITVLDKWTKM-GEFEFLTHLRK 190

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDIL 248
           LTF+II  IF+ S S E+ +  +E  ++ +  G  ++ +N PGF  H+ALKARK L+   
Sbjct: 191 LTFRIIMYIFLSSES-ENVMDALEREYTALNYGVRAMAVNIPGFAYHRALKARKTLVAAF 250

Query: 249 QSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+ ++R  RK+   S   KDM+D L+ VKDEDG+ LDDE I D++   L AGHE+S +
Sbjct: 251 QSIVTERRNQRKQNILS-NKKDMLDNLLNVKDEDGKTLDDEEIIDVLLMYLNAGHESSGH 310

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGG 368
           T MWA +FL +HP + QRAK EQE+I+  RP  QKG+ L E ++M+FLSQ++DETLRV  
Sbjct: 311 TIMWATVFLQEHPEVLQRAKAEQEMILKSRPEGQKGLSLKETRKMEFLSQVVDETLRVIT 370

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDN-YVATPG 428
            +L  FREA  DVE+NG +IPKGWKV+ WF ++++D ++FP PR+F+P+RWDN +V   G
Sbjct: 371 FSLTAFREAKTDVEMNGYLIPKGWKVLTWFRDVHIDPEVFPDPRKFDPARWDNGFVPKAG 430

Query: 429 AFIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           AF+PFG+GS  CPG+DLAKLEISIFLH+FLL Y++++ NP+CP+ Y+P   P D CLAR+
Sbjct: 431 AFLPFGAGSHLCPGNDLAKLEISIFLHHFLLKYQVKRSNPECPVMYLPHTRPTDNCLARI 487

[Close]

BLAST of CmaCh16G010720 vs. TAIR 10
Match: AT2G32440.1 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 464.9 bits (1195), Expect = 7.9e-131
Identity = 233/479 (48.64%), Postives = 322/479 (67.22%), Query Frame = 0


Query: 9   WLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQP 68
           W  LI+     +  +LKR+N W Y +KLG+    LPPGD+GWP++G+  S+++ F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  RK Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQRK-QNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGG 368
             MWA + + +HP I Q+AKEEQE IV +R   QK + L E + M +LSQ+IDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

[Close]

BLAST of CmaCh16G010720 vs. TAIR 10
Match: AT2G32440.2 (ent-kaurenoic acid hydroxylase 2 )

HSP 1 Score: 464.9 bits (1195), Expect = 7.9e-131
Identity = 233/479 (48.64%), Postives = 322/479 (67.22%), Query Frame = 0


Query: 9   WLVLIVAAFVFVLGILKRLNGWYYAAKLGKTWPKLPPGDMGWPLLGSTISYIKNFISGQP 68
           W  LI+     +  +LKR+N W Y +KLG+    LPPGD+GWP++G+  S+++ F +  P
Sbjct: 10  WFPLIILGLFVLKWVLKRVNVWIYVSKLGEKKHYLPPGDLGWPVIGNMWSFLRAFKTSDP 69

Query: 69  SNFIANFYIRYGKRDMYKTHLLGRATIIVCSSELCRQVLGDEDNFKPSLPKNINMLTGRK 128
            +FI ++  RYG+  +YK H+ G   ++V + E CR+VL D+D F    PK+   L GRK
Sbjct: 70  ESFIQSYITRYGRTGIYKAHMFGYPCVLVTTPETCRRVLTDDDAFHIGWPKSTMKLIGRK 129

Query: 129 SLVQVFKAEHKRLRRLTMAPISGHAALEMYINHIEHTVMSGLEEWASMKNPIELLSEIKK 188
           S V +   EHKRLRRLT AP++G  AL +YI  IE TV + LE+W+ M   IE LS ++K
Sbjct: 130 SFVGISFEEHKRLRRLTSAPVNGPEALSVYIQFIEETVNTDLEKWSKM-GEIEFLSHLRK 189

Query: 189 LTFKIIWNIFMGSSSLESSIGEMEGLFSEVALGFISLPINFPGFNLHKALKARKKLLDIL 248
           LTFK+I  IF+ S S E  +  +E  ++ +  G  ++ IN PGF  H+ALKARKKL+   
Sbjct: 190 LTFKVIMYIFLSSES-EHVMDSLEREYTNLNYGVRAMGINLPGFAYHRALKARKKLVAAF 249

Query: 249 QSIIDQKRLVRKRQGTSWEAKDMMDLLMEVKDEDGEELDDETIRDLIFGKLFAGHETSAY 308
           QSI+  +R  RK Q  S   KDM+D L++VKDE+G  LDDE I DL+   L AGHE+S +
Sbjct: 250 QSIVTNRRNQRK-QNISSNRKDMLDNLIDVKDENGRVLDDEEIIDLLLMYLNAGHESSGH 309

Query: 309 TAMWAVLFLTKHPHIFQRAKEEQELIVAQRPTTQKGIILSEVKRMKFLSQIIDETLRVGG 368
             MWA + + +HP I Q+AKEEQE IV +R   QK + L E + M +LSQ+IDETLRV  
Sbjct: 310 LTMWATILMQEHPMILQKAKEEQERIVKKRAPGQK-LTLKETREMVYLSQVIDETLRVIT 369

Query: 369 VTLMLFREAVVDVEINGKIIPKGWKVIPWFGELYMDEKLFPSPREFNPSRWDNYVATPGA 428
            +L  FREA  DV+++G IIPKGWKV+ WF  +++D +++P P++F+PSRW+ Y    G 
Sbjct: 370 FSLTAFREAKSDVQMDGYIIPKGWKVLTWFRNVHLDPEIYPDPKKFDPSRWEGYTPKAGT 429

Query: 429 FIPFGSGSRYCPGSDLAKLEISIFLHYFLLNYKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           F+PFG GS  CPG+DLAKLEISIFLH+FLL Y++E+ NP CP+ ++P   PKD CLAR+
Sbjct: 430 FLPFGLGSHLCPGNDLAKLEISIFLHHFLLKYRVERSNPGCPVMFLPHNRPKDNCLARI 484

[Close]

BLAST of CmaCh16G010720 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 149/448 (33.26%), Postives = 242/448 (54.02%), Query Frame = 0


Query: 42  KLPPGDMGWPLLGSTIS-YIKNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSS 101
           +LPPG MG P +G T+  Y +N     P++F A    +YG  D++KTH+LG   +++ S 
Sbjct: 48  RLPPGSMGLPYIGETLRLYTEN-----PNSFFATRQNKYG--DIFKTHILGCPCVMISSP 107

Query: 102 ELCRQVLGDEDN-FKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYI 161
           E  R VL  + + FKP+ P +   + G ++L       H  L+RL  +     +AL   +
Sbjct: 108 EAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTV 167

Query: 162 NHIEHTVMSGLEEWASMKNPIELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVA 221
           +HIE  V+  L  W S K+ I  L  +K+  F +      G     ++I  ++ L+  + 
Sbjct: 168 SHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLE 227

Query: 222 LGFISLPINFPGFNLHKALKARKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVK 281
            G+ S+P++ PG   HK++KAR +L + L+ +I+++R   + +G       ++ +L+  K
Sbjct: 228 RGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAK 287

Query: 282 DEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRP 341
           D+    L D  I D I G +FA  +T+A    W + +L  HP++ Q    EQ  I  +  
Sbjct: 288 DQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIK 347

Query: 342 TTQKGIILSEVKRMKFLSQIIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFG 401
              + I   + ++M   +++I ETLR   V    FREAV DVE +G +IPKGWKV+P F 
Sbjct: 348 KENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFR 407

Query: 402 ELYMDEKLFPSPREFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLN 461
            ++   + FP P +F+PSR++     P  ++PFG+G   CPGS+LAKLE+ I LH+   +
Sbjct: 408 RIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTS 467

Query: 462 YKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           ++ E +  +  + Y PFP PK     RV
Sbjct: 468 FRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

[Close]

BLAST of CmaCh16G010720 vs. TAIR 10
Match: AT2G29090.2 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 149/448 (33.26%), Postives = 242/448 (54.02%), Query Frame = 0


Query: 42  KLPPGDMGWPLLGSTIS-YIKNFISGQPSNFIANFYIRYGKRDMYKTHLLGRATIIVCSS 101
           +LPPG MG P +G T+  Y +N     P++F A    +YG  D++KTH+LG   +++ S 
Sbjct: 48  RLPPGSMGLPYIGETLRLYTEN-----PNSFFATRQNKYG--DIFKTHILGCPCVMISSP 107

Query: 102 ELCRQVLGDEDN-FKPSLPKNINMLTGRKSLVQVFKAEHKRLRRLTMAPISGHAALEMYI 161
           E  R VL  + + FKP+ P +   + G ++L       H  L+RL  +     +AL   +
Sbjct: 108 EAARMVLVSKAHLFKPTYPPSKERMIGPEALFFHQGPYHSTLKRLVQSSFM-PSALRPTV 167

Query: 162 NHIEHTVMSGLEEWASMKNPIELLSEIKKLTFKIIWNIFMGSSSLESSIGEMEGLFSEVA 221
           +HIE  V+  L  W S K+ I  L  +K+  F +      G     ++I  ++ L+  + 
Sbjct: 168 SHIELLVLQTLSSWTSQKS-INTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLE 227

Query: 222 LGFISLPINFPGFNLHKALKARKKLLDILQSIIDQKRLVRKRQGTSWEAKDMMDLLMEVK 281
            G+ S+P++ PG   HK++KAR +L + L+ +I+++R   + +G       ++ +L+  K
Sbjct: 228 RGYNSMPLDLPGTLFHKSMKARIELSEELRKVIEKRRENGREEG------GLLGVLLGAK 287

Query: 282 DEDGEELDDETIRDLIFGKLFAGHETSAYTAMWAVLFLTKHPHIFQRAKEEQELIVAQRP 341
           D+    L D  I D I G +FA  +T+A    W + +L  HP++ Q    EQ  I  +  
Sbjct: 288 DQKRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIK 347

Query: 342 TTQKGIILSEVKRMKFLSQIIDETLRVGGVTLMLFREAVVDVEINGKIIPKGWKVIPWFG 401
              + I   + ++M   +++I ETLR   V    FREAV DVE +G +IPKGWKV+P F 
Sbjct: 348 KENRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDVEYDGYLIPKGWKVLPLFR 407

Query: 402 ELYMDEKLFPSPREFNPSRWDNYVATPGAFIPFGSGSRYCPGSDLAKLEISIFLHYFLLN 461
            ++   + FP P +F+PSR++     P  ++PFG+G   CPGS+LAKLE+ I LH+   +
Sbjct: 408 RIHHSSEFFPDPEKFDPSRFE-VAPKPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTTS 467

Query: 462 YKIEQLNPKCPMTYMPFPHPKDKCLARV 488
           ++ E +  +  + Y PFP PK     RV
Sbjct: 468 FRWEVIGDEEGIQYGPFPVPKKGLPIRV 479

The following BLAST results are available for this feature:

BLAST of CmaCh16G010720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B5BSX1	1.1e-145	50.41	Beta-amyrin 11-oxidase OS=Glycyrrhiza uralensis OX=74613 GN=CYP88D6 PE=1 SV=1	[more]
O23051	3.7e-133	48.33	Ent-kaurenoic acid oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=KAO1 PE=1 SV=1	[more]
Q9C5Y2	1.1e-129	48.64	Ent-kaurenoic acid oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=KAO2 PE=1 SV=2	[more]
Q5VRM7	3.9e-119	43.54	Ent-kaurenoic acid oxidase OS=Oryza sativa subsp. japonica OX=39947 GN=KAO PE=2 ...	[more]
Q43246	2.0e-118	43.49	Cytochrome P450 88A1 OS=Zea mays OX=4577 GN=CYP88A1 PE=2 SV=1	[more]

BLAST of CmaCh16G010720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05160.1	2.6e-134	48.33	cytochrome P450, family 88, subfamily A, polypeptide 3	[more]
AT2G32440.1	7.9e-131	48.64	ent-kaurenoic acid hydroxylase 2	[more]
AT2G32440.2	7.9e-131	48.64	ent-kaurenoic acid hydroxylase 2	[more]
AT2G29090.1	3.0e-66	33.26	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]
AT2G29090.2	3.0e-66	33.26	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 430..439 score: 56.28 coord: 358..369 score: 32.2 coord: 439..450 score: 39.48 coord: 301..318 score: 34.66
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 44..470 e-value: 2.7E-67 score: 227.5
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 310..336 score: 26.66 coord: 429..439 score: 53.91 coord: 439..462 score: 30.53 coord: 397..421 score: 27.12 coord: 290..307 score: 25.14
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 35..490 e-value: 1.7E-105 score: 355.4
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 41..487
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 22..468
None	No IPR available	PANTHER	PTHR24286:SF199	CYTOCHROME P450 88D6	coord: 22..468
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 432..441

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010720.1	CmaCh16G010720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen