CmaCh16G010560 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G010560
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionexopolygalacturonase-like
LocationCma_Chr16: 8092169 .. 8093992 (+)
RNA-Seq ExpressionCmaCh16G010560
SyntenyCmaCh16G010560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGCATTTCACACAAACAATCTCCAAAAACAAAAACAAAAACAAAAAAAAGACAACAAAACAAAACAAAAAAAAAACAAAACAAAACAACAAAGCTGAAGAACAAGAAGATGGGTATGAACATGAGGTCGATAACTCTATCGTTCGTGTTATTATTATACGTTTCTATAAGAACAGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGGTAAAAACTAATATCAAATATATCGATGTTTATATATTTTTACGATGATAAATCTTTTCTAATTATGTTCGAGATTTTAAACAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACATGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGGCAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGGTAAATACACTTAAATTAACTATTTGCAAATTTTAATTTATTGTTTATACTCATGCATGATCGTTTTTTTACAGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTCGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTTAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTCTGGATCAAGAATACTGTCCATACAACAAATGTAACAGGAAGGTACTGGAATTACGAAATCTTTTATTTTTTTTATTTTATTTTAATTAGAAACAATTTCATTAAAATTGTGTTTATTTGATGTTTTTCAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGCAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGGTTGTTACTGGGATACAAAAACCTATTATTTGTCCTTCGTGAGCGTAAGCTATTTCTACAAAAGATTATCGTTTGAGGAAGAGATTTAATTATGTAGTTTTCTTTTACGTTCTAGAGAATAAATTTGGAGTGAACAAAGGGTTCTCCTTCGTTGTATTTATAAATGAAGAGGAATAAAGCTTTGGTTTGTGTTGATTTTTCATGTGAAGCCGTTGTATTGAGATCACTTATAATTGGATTTGTAGCATTTTCGATCAAACGGGTGATTTTCTCATCTACTACACATCTCATACTACCCCTGTGAACTTAAAATTCATAATCCCA

mRNA sequence

CAAGCATTTCACACAAACAATCTCCAAAAACAAAAACAAAAACAAAAAAAAGACAACAAAACAAAACAAAAAAAAAACAAAACAAAACAACAAAGCTGAAGAACAAGAAGATGGGTATGAACATGAGGTCGATAACTCTATCGTTCGTGTTATTATTATACGTTTCTATAAGAACAGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACATGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGGCAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTCGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTTAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTCTGGATCAAGAATACTGTCCATACAACAAATGTAACAGGAAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGCAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGGTTGTTACTGGGATACAAAAACCTATTATTTGTCCTTCGTGAGCGTAAGCTATTTCTACAAAAGATTATCGTTTGAGGAAGAGATTTAATTATGTAGTTTTCTTTTACGTTCTAGAGAATAAATTTGGAGTGAACAAAGGGTTCTCCTTCGTTGTATTTATAAATGAAGAGGAATAAAGCTTTGGTTTGTGTTGATTTTTCATGTGAAGCCGTTGTATTGAGATCACTTATAATTGGATTTGTAGCATTTTCGATCAAACGGGTGATTTTCTCATCTACTACACATCTCATACTACCCCTGTGAACTTAAAATTCATAATCCCA

Coding sequence (CDS)

ATGGGTATGAACATGAGGTCGATAACTCTATCGTTCGTGTTATTATTATACGTTTCTATAAGAACAGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACATGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGGCAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTCGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTTAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTCTGGATCAAGAATACTGTCCATACAACAAATGTAACAGGAAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGCAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGGTTGTTACTGGGATACAAAAACCTATTATTTGTCCTTCGTGA

Protein sequence

MGMNMRSITLSFVLLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVDITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVVTGIQKPIICPS
Homology
BLAST of CmaCh16G010560 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 2.3e-122
Identity = 203/355 (57.18%), Postives = 265/355 (74.65%), Query Frame = 0

Query: 42  DITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVD--- 101
           D TQ L  AWK+ACAS   S +++PK  + +G+ITL GPCKS I + +QGT+ AP D   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 102 ITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKN-PKCARIPMSLKFSFVKDSIV 161
           I G GWI    +D +++ GGGVFDGQG+ AW +NDCHKN P C  + M+L+   V +SI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 162 RDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDD 221
           RD+T+ DSKNFHVN++GCKNLTF+   I+A  TSIN+DGIH+GR DGVNIIN+ I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 222 CISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTW 281
           CISLGDGS+ + ITN+TCGPGHGISVGSLG+Y  EE V G+YVKNCT+ G+ NG+RIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 282 PASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSA 341
           P S   G  ++MH++DI +  V  PI++DQ YCPYN+C  ++PS +K+SK+SFKNI+G++
Sbjct: 241 PKS-EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 342 GNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVVTGIQKPIIC 393
               AVKL+CS  +PC  V L+DID+TY+G+ GP  S C N+KP + G Q P IC
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAIC 354

BLAST of CmaCh16G010560 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 5.6e-116
Identity = 196/375 (52.27%), Postives = 263/375 (70.13%), Query Frame = 0

Query: 21  RTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGP 80
           R  QSS  VF+V  YGAK   DI+Q + KAWK ACAS   S ++IPK  Y +G + + GP
Sbjct: 1   RGVQSSGSVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGP 60

Query: 81  CK-SPIDIVVQGTIMAPVD---ITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHK 140
           CK S I   + G + AP D      DGW+ F  +D ++++G G  DGQGQ AW +N+C K
Sbjct: 61  CKGSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDK 120

Query: 141 NPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDG 200
           NP C    M+L+F F+K ++VRDITS +SK FH+N+L C+++TF HVT+ APGTSIN+DG
Sbjct: 121 NPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDG 180

Query: 201 IHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVA 260
           IH+G   GV I N+ I TGDDCIS+G GS+ VTIT V CGPGHGIS+GSLG+Y  E+ V 
Sbjct: 181 IHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVR 240

Query: 261 GLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCN 320
           G+ VK CT  GT NG+R+KTWP SP  G+ TD+ ++D+ + +VQNP+ILDQEYCPY +C+
Sbjct: 241 GITVKGCTFSGTMNGVRVKTWPNSP-PGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCS 300

Query: 321 RKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSEC 380
           R+ PS+IK+S ++F NIRG++   VAV + CS   PC ++ + +I+++Y G  GP  S C
Sbjct: 301 RQAPSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTC 360

Query: 381 SNVKPVVTGIQKPII 392
           SNVKP  +G Q P I
Sbjct: 361 SNVKPTFSGKQVPAI 374

BLAST of CmaCh16G010560 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-114
Identity = 200/389 (51.41%), Postives = 279/389 (71.72%), Query Frame = 0

Query: 5   MRSITLSFVLLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLI 64
           M+  T   V  L+++   A  S GV D++ +G KPN+DI Q L  AW +ACAS   +K++
Sbjct: 1   MKFSTAIIVSFLFIADFCAAQS-GVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIV 60

Query: 65  IPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVD---ITG-DGWIQFRYVDKISLTGGGV 124
           IP  TY L  I L GPCK+PI++ V GTI AP D   I G + W +F Y+D ++L+G GV
Sbjct: 61  IPAGTYQLNGIELKGPCKAPIELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGV 120

Query: 125 FDGQG----QKAWERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCK 184
           FDGQG    +KA   +        +++ M+  F+FV +SIVR +TSKDSKNFHV + GCK
Sbjct: 121 FDGQGATVYKKAAPASAWSGKNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCK 180

Query: 185 NLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCG 244
           N+TFD  TI APG S N+DGIHMG+   V I+N+ IGTGDDC+S+GDGS+++T+  V CG
Sbjct: 181 NITFDGFTITAPGDSPNTDGIHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCG 240

Query: 245 PGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVL 304
           PGHG+SVGSLG++  EE V G+ VKNCTL  T NG+RIKTWP +P T +V+D+H+EDI +
Sbjct: 241 PGHGLSVGSLGKFTTEENVEGITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITM 300

Query: 305 VDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDV 364
            +V+NP+I+DQEY P+N+C++K PSKIK+SK+SFKN++G++G A  V L+CS+  PC+ V
Sbjct: 301 TNVKNPVIIDQEYYPWNQCSKKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGV 360

Query: 365 TLSDIDITYTGREGPVVSECSNVKPVVTG 386
            L+++D+ + G   P  ++C+NVKP+VTG
Sbjct: 361 ELNNVDLKFNG--APTTAKCTNVKPLVTG 386

BLAST of CmaCh16G010560 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 9.9e-113
Identity = 185/364 (50.82%), Postives = 257/364 (70.60%), Query Frame = 0

Query: 31  DVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPIDIVVQ 90
           +V T G  PN+DIT  + KA+  AC +P  S+++IPK  + LG   + GPCKSPI+  +Q
Sbjct: 23  EVFTAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQ 82

Query: 91  GTIMAPVDIT--GDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLK 150
           G +      T   D W+ F  ++   L GGG FDG+G  AW+ N+CHK  +C ++P+S++
Sbjct: 83  GNVKTDGGSTQGKDRWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVR 142

Query: 151 FSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNII 210
           F FV ++ ++D+TS D+KNFH N++  KN+TFD++ I AP  S N+DGIH+GRC+GV I+
Sbjct: 143 FDFVDNAEIKDVTSLDAKNFHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKIL 202

Query: 211 NSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGT 270
           N+ I TGDDCIS+GDG + + I  V CGPGHGISVGSLG+Y  E+ V  + VKNCTL GT
Sbjct: 203 NTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGT 262

Query: 271 TNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKV 330
           +NGLRIKTWP++  T +   +H+EDI+L  V NPI++DQEYCP+N+CN+  PS IK+  +
Sbjct: 263 SNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDI 322

Query: 331 SFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVVTGIQK 390
           +F+NIRG++ N  AVKLLCS  +PCE+V + DI+I YTG +GP   EC+NV P + G Q 
Sbjct: 323 TFRNIRGTSENKDAVKLLCSKGHPCENVEIGDINIEYTGPDGPPTFECTNVTPKLVGAQN 382

Query: 391 PIIC 393
           P  C
Sbjct: 383 PKAC 386

BLAST of CmaCh16G010560 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.6e-110
Identity = 189/384 (49.22%), Postives = 265/384 (69.01%), Query Frame = 0

Query: 12  FVLLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYM 71
           F L+L       +S  GVFD+T YGA  NADI++ L  A+K+AC S   S ++IPK T+ 
Sbjct: 9   FALVLLFLAHFGESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFT 68

Query: 72  LGVITLLGPCKSPIDIVVQGTIMAPVD---ITGDGWIQFRYVDKISLTGGGVFDGQGQKA 131
           +  + L GPCKSP+++ +Q T+ AP D   +    W+    +D+ +++GGG+ DGQ   A
Sbjct: 69  MNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAA 128

Query: 132 WERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAP 191
           WE   C ++ KC ++P +L F+ + +S ++DIT+ DSK+FHVN+  CKNLTF    ++AP
Sbjct: 129 WE---CKQSKKCNKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAP 188

Query: 192 GTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQ 251
             S N+DGIH+ R   VNI +S   TGDDCIS+GD + ++ IT VTCGPGHGISVGSLG 
Sbjct: 189 ANSPNTDGIHVSRSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGG 248

Query: 252 YLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQE 311
              E+PV G++V+NCT   T NG+RIKTWPAS   G V D+H+EDI++ +V NP+++DQ 
Sbjct: 249 NPDEKPVVGVFVRNCTFTNTDNGVRIKTWPAS-HPGVVNDVHFEDIIVQNVSNPVVIDQV 308

Query: 312 YCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGR 371
           YCP+NKCN+ +PS++KISKVSF+NI+G++    AV LL S   PCE + + DIDITY+G+
Sbjct: 309 YCPFNKCNKDLPSQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGK 368

Query: 372 EGPVVSECSNVKPVVTGIQKPIIC 393
           EGP  S C N+KP + G Q P +C
Sbjct: 369 EGPAKSSCENIKPSLKGKQNPPVC 388

BLAST of CmaCh16G010560 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 417.5 bits (1072), Expect = 1.2e-116
Identity = 188/357 (52.66%), Postives = 261/357 (73.11%), Query Frame = 0

Query: 39  PNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPIDIVVQGTIMAPVD 98
           P +DIT  L KA+ +AC  P +S ++IPK  Y LG I ++GPCK+PI I + GT+ A  +
Sbjct: 32  PGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGN 91

Query: 99  ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIV 158
             G + W+ FR ++   L GGGVFDG+G  AW  N+CHK   C ++P+S++F FV D+ +
Sbjct: 92  ANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKI 151

Query: 159 RDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDD 218
           R ITS D+K+FH+N++G KN+TF+ V I AP  S N+DGIH+GR DG+ IINS I TGDD
Sbjct: 152 RGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDD 211

Query: 219 CISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTW 278
           C+S+GDG + + +  VTCGPGHGIS+GSLG+Y  EE V+G+ + NCTL  T NGLRIKTW
Sbjct: 212 CVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTW 271

Query: 279 PASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSA 338
           P++  T + +D+H+E+I+L +V NPI++DQEYCP+N+CN++ PS IK++ +SFK IRG++
Sbjct: 272 PSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTS 331

Query: 339 GNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVVTGIQKPIICPS 395
           GN  AVKLLCS  YPC++V + D++I YTG +GP   +CSNV P + G Q P  C S
Sbjct: 332 GNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKACSS 388

BLAST of CmaCh16G010560 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 2.0e-116
Identity = 193/364 (53.02%), Postives = 263/364 (72.25%), Query Frame = 0

Query: 31  DVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPIDIVVQ 90
           +V T G+   +DITQ L KA+  AC S   SK++IPK  + LG I + GPCK+PI++ +Q
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 91  GTIMAPVD-ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLK 150
           GT+ A  + I G + W+ F  +D   L GGG FDG+G  AW  N+CHK  +C ++P+S++
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 142

Query: 151 FSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNII 210
           F F+ +S +RDI+S D+KNFH+N+LG KN+T +++ I AP  S N+DGIH+GR DGV I+
Sbjct: 143 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 202

Query: 211 NSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGT 270
           NS I TGDDCIS+GDG + + +  VTCGPGHGISVGSLG+Y  E+ V+G+ V NCTL  T
Sbjct: 203 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 262

Query: 271 TNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKV 330
            NGLRIKTWP++  + + +D+H+EDI+L DV NPI++DQEYCP+N+CN++  S IK+  +
Sbjct: 263 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 322

Query: 331 SFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVVTGIQK 390
           SFKNIRG++GN  AVKLLCS  YPC++V + DIDI Y G +GP    CSNV P + G Q 
Sbjct: 323 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQS 382

Query: 391 PIIC 393
           P  C
Sbjct: 383 PKAC 386

BLAST of CmaCh16G010560 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 408.3 bits (1048), Expect = 7.0e-114
Identity = 191/381 (50.13%), Postives = 272/381 (71.39%), Query Frame = 0

Query: 14  LLLYVSIRTAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLG 73
           +L  +S+    ++  VF+V   G  P +DITQ L KA+  AC +P  SK++I K  + LG
Sbjct: 10  VLFVISLLGFSANAEVFNV---GGSPGSDITQALLKAFTSACQAPTASKVVITKGEFKLG 69

Query: 74  VITLLGPCKSPIDIVVQGTIMAP-VDITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWER 133
            I + GPCK+P++I +QGT+ A    I G + W+ F  ++   L GGG+FDG+G  AW  
Sbjct: 70  EIEMTGPCKAPVEINLQGTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAAWRV 129

Query: 134 NDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTS 193
           N+CHK  +C ++P+S++F FV+++ +RDI+S D+KNFH+N+LG KN+TFD+V + AP  S
Sbjct: 130 NNCHKTFECKKLPISIRFDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAES 189

Query: 194 INSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLV 253
            N+DGIH+GR +GV I+NS I TGDDCIS+GDG + + + NV CGPGHGISVGSLG+Y+ 
Sbjct: 190 PNTDGIHLGRSEGVKILNSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVH 249

Query: 254 EEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCP 313
           E+ V G+ V NCTL GT NGLRIKTWP++    + + +H+E+I+L +V NPI++DQEYCP
Sbjct: 250 EQDVTGITVVNCTLQGTDNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCP 309

Query: 314 YNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGP 373
           +N+CN++ PS IK+  +SFKNIRG++GN  AVKLLCS  +PC +V + +I++ Y G +GP
Sbjct: 310 WNQCNKQKPSTIKLVDISFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGP 369

Query: 374 VVSECSNVKPVVTGIQKPIIC 393
               CSNV P + G Q P  C
Sbjct: 370 PTFMCSNVSPKLVGTQNPKAC 387

BLAST of CmaCh16G010560 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.5e-113
Identity = 189/369 (51.22%), Postives = 266/369 (72.09%), Query Frame = 0

Query: 26  SDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGPCKSPI 85
           S    +V    + P +DITQ L +A+  AC SP   K++IPK  + LG I + GPCKSP+
Sbjct: 19  SANAHEVLRLTSSPGSDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPV 78

Query: 86  DIVVQGTIMAPVD-ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARI 145
           +I + GT++A  + I G + W+ F+ +D   L GGG FDG+G  AW  N+CHK  +C ++
Sbjct: 79  EITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKL 138

Query: 146 PMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCD 205
           P+S++F FV ++ +RDI+S D+KNFH+N++G KN+TFD+V I AP  S N+DGIH+GR  
Sbjct: 139 PISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSV 198

Query: 206 GVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNC 265
           GV+IINS I TGDDC+S+GDG   + + NV CGPGHGISVGSLG+Y  E+ V+G+ V NC
Sbjct: 199 GVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINC 258

Query: 266 TLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKI 325
           TL  T NGLRIKTWP++  + + +++H+E+I+L +V NPI++DQEYCP+N+CN++  S I
Sbjct: 259 TLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSI 318

Query: 326 KISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPVV 385
           K++ +SF+ IRG++GN  AVKLLCS  YPCE+V + DI+I YTG +GP    CSNV+P +
Sbjct: 319 KLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPKL 378

Query: 386 TGIQKPIIC 393
            G Q P  C
Sbjct: 379 VGTQFPKAC 387

BLAST of CmaCh16G010560 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 6.4e-99
Identity = 169/377 (44.83%), Postives = 246/377 (65.25%), Query Frame = 0

Query: 23  AQSSDGVFDVTTYGAKPN--ADITQILDKAWKDACASPIQSKLIIPKETYMLGVITLLGP 82
           A+      DV   GAK +   D +     AWK+ACA+   S + +PK  Y++  +   GP
Sbjct: 64  AKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEACAA--GSTITVPKGEYLVESLEFKGP 123

Query: 83  CKSPIDIVVQGTIMAPVDITGD----GWIQFRYVDKISLTGG-GVFDGQGQKAWERNDCH 142
           CK P+ + + G   AP  +       GWI F  +   +L G   +FDGQG  AW+ NDC 
Sbjct: 124 CKGPVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCA 183

Query: 143 KNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSD 202
           K  KC  +P++++F+ + +S +  ITS +SK FH+NIL CKN+T   + I+AP  S+N+D
Sbjct: 184 KTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTD 243

Query: 203 GIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPV 262
           GIH+GR +GVN+I + I TGDDC+S+GDG+  + + NV CGPGHGIS+GSLG+Y  E+PV
Sbjct: 244 GIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPV 303

Query: 263 AGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKC 322
            G+ V+ C +  T NG+RIKTWP SP  G  +++ +EDI + +V  P+++DQEYCPY  C
Sbjct: 304 KGVTVRKCLIKNTDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHC 363

Query: 323 NRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSE 382
              +PSK+K+S V+ KNI+G++   VAVKL+CS   PC ++ LSDI++ + G+EGP VS 
Sbjct: 364 KAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSA 423

Query: 383 CSNVKPVVTGIQKPIIC 393
           CSN+KP+++G   P  C
Sbjct: 424 CSNIKPILSGKLVPAAC 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245482.3e-12257.18Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q6H9K05.6e-11652.27Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q403121.8e-11451.41Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
P353379.9e-11350.82Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q059671.6e-11049.22Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G48140.11.2e-11652.66Pectin lyase-like superfamily protein [more]
AT3G07820.12.0e-11653.02Pectin lyase-like superfamily protein [more]
AT3G07840.17.0e-11450.13Pectin lyase-like superfamily protein [more]
AT3G07830.13.5e-11351.22Pectin lyase-like superfamily protein [more]
AT3G14040.16.4e-9944.83Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 255..276
e-value: 29.0
score: 13.6
coord: 353..375
e-value: 4700.0
score: 2.4
coord: 320..345
e-value: 9100.0
score: 0.4
coord: 225..245
e-value: 2100.0
score: 4.9
coord: 175..201
e-value: 210.0
score: 10.7
coord: 202..223
e-value: 1600.0
score: 5.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 18..390
e-value: 1.7E-105
score: 355.3
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 55..380
e-value: 1.2E-99
score: 333.5
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 232..245
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 15..393
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 15..393
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 17..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G010560.1CmaCh16G010560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity