CmaCh16G010550 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G010550
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPectin lyase-like superfamily protein
LocationCma_Chr16: 8082866 .. 8085166 (+)
RNA-Seq ExpressionCmaCh16G010550
SyntenyCmaCh16G010550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTATGAACATGAAGTCGATAACTCTATCGTTCGTATTATTATTATACGTTTCTATAAAAACAGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGGTAAAAACTAATATCAAATATATCGATGTTTATATATTTTTACGATGATAAATCTTTTCTAATTATGTTCGATATTTTAAACAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACACGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGACAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGGTAAATACACTTAAATTAACTATTTGCAAATTTTAATTTATTGTTTATACTCATGCATGATCGTTTTTTTACAGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTTGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTGAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTTTGGATCAAGAATATTGTCCATACAACAAATGTAACAGGAAGGTACTGGAATTACGAAATCTTTTATTTTCTTTATTTTATTTTAATTAGAAACAATTTCATTAAAATTGTGTTTATTTGATGTTTTTTAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGGAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGGTTGTTACTGGGATACAAAAACCTATTATTTGTCCTTCGTGAGTGTAAGCTATTTCTACAAAAGATTATCGTTTGAATATGTAGTTTTCTTTTACGTTCTAGAAAATAAATTTGGAGTGAACAAAGGGTTCTCCTTCGTTGTATTTATAAATGAAGAGGAATAAAGCTTTGGTTTTGTGTTGATTTTTCATGTGAAGCTGTTGAGTTAGCTGATATTGAGATCACTTATAATTGGATTTGTAGCCGGGTGATTTTCTCATCTACTACACATCTCATACTACCCCTGTGAACTTAAAATTCATAATCCCAATTTAATTTACCTGTGCCTGTTTGAACAACCTCAGTTAATCGTGTCTTTATTTGATAAGAATCTGTACATCTTTATAAGAAAACTTTATCCATGGATCCCTTCCATGGAAGAAGTGCATCTTTATAATTCGTGATAATTTTTTCAATTTCGAACTTATTTTTTAATACAAATCATGAGAATTTATATTTTTTCTCTAATCTTTCGTTGAGAGGTAAAGGACTAAATCATTTTAAGAAAAAAAGTTTTTGAAAGGAATATTAATAATTTCAAAGGATCCTTGAACTTTTACTACATTTTCAAGTGAAGACATATATGGAACCTCAAAATGAAACCTTTCATGACTTATATGAAACCAATGAAACCTTTCATGACATATATGAAACCTCAAACGATGAGTCTGTAATTTCAAAATGAAACCTTTCTTGAAGATTAATGTTTTAGTTCATATTAAAAGGCTCAATTTTATCTCAATGTTCAAATGTGAAACTCAAATTAAAAGCCATTTCATTTATTTTACCACTCAATTTCTAAGATAAATAAGTTGAAATGAACCAAGGGATTTCCTTTATTTTATTTATAAATGAAGAGGCATGCAACTTAA

mRNA sequence

ATGGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACACGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGACAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTTGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTGAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTTTGGATCAAGAATATTGTCCATACAACAAATGTAACAGGAAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGGAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGAGGCATGCAACTTAA

Coding sequence (CDS)

ATGGCTCAATCAAGTGATGGAGTTTTTGACGTGACAACATACGGTGCTAAGCCTAATGCTGATATAACTCAGATTCTAGACAAGGCATGGAAGGACGCTTGTGCTTCACCGATTCAAAGTAAATTAATTATTCCAAAAGAGACTTACACGTTAGGTGTAATCACATTGCTTGGTCCTTGCAAGAGTCCAATTGATATTGTCGTGCAAGGAACAATAATGGCACCCGTAGATATAACAGGTGATGGTTGGATTCAGTTCAGATATGTCGACAAAATTTCATTAACAGGTGGCGGAGTTTTTGATGGACAAGGACAAAAAGCTTGGGAAAGAAACGATTGTCATAAAAACCCTAAGTGTGCAAGAATTCCAATGAGTTTGAAGTTCAGCTTTGTAAAAGATTCAATTGTGAGGGACATAACGTCAAAAGACAGCAAGAATTTTCATGTCAATATCCTAGGTTGTAAGAATCTTACTTTCGACCACGTGACTATAAATGCACCCGGAACTAGTATTAATAGTGATGGAATTCACATGGGTCGTTGTGATGGAGTTAACATCATCAATTCAGTTATTGGTACTGGTGATGATTGTATCTCACTTGGAGATGGAAGCAGAGAGGTGACAATTACGAACGTGACGTGTGGACCTGGACACGGCATAAGCGTAGGAAGTCTAGGACAATATCTTGTTGAAGAACCGGTTGCTGGGCTTTATGTCAAAAATTGTACATTAATTGGTACCACAAATGGCCTGAGAATCAAAACATGGCCGGCTTCTCCTGCTACTGGATCGGTTACTGATATGCACTATGAAGATATAGTCTTGGTTGACGTCCAAAATCCAATTATTTTGGATCAAGAATATTGTCCATACAACAAATGTAACAGGAAGATTCCGTCGAAAATCAAGATAAGTAAGGTGAGTTTCAAGAATATTAGAGGTAGTGCTGGGAATGCAGTTGCTGTGAAGCTTTTGTGTAGTGCTGATTATCCATGTGAAGATGTGACGTTATCTGACATTGATATCACTTATACCGGACGTGAAGGTCCAGTTGTATCTGAATGTTCGAATGTGAAACCGAGGCATGCAACTTAA

Protein sequence

MAQSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQGTIMAPVDITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPRHAT
Homology
BLAST of CmaCh16G010550 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 7.7e-120
Identity = 198/344 (57.56%), Postives = 259/344 (75.29%), Query Frame = 0

Query: 21  DITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQGTIMAPVD--- 80
           D TQ L  AWK+ACAS   S +++PK  + +G+ITL GPCKS I + +QGT+ AP D   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSK 60

Query: 81  ITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKN-PKCARIPMSLKFSFVKDSIV 140
           I G GWI    +D +++ GGGVFDGQG+ AW +NDCHKN P C  + M+L+   V +SI+
Sbjct: 61  IKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSIL 120

Query: 141 RDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDD 200
           RD+T+ DSKNFHVN++GCKNLTF+   I+A  TSIN+DGIH+GR DGVNIIN+ I TGDD
Sbjct: 121 RDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDD 180

Query: 201 CISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTW 260
           CISLGDGS+ + ITN+TCGPGHGISVGSLG+Y  EE V G+YVKNCT+ G+ NG+RIKTW
Sbjct: 181 CISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTW 240

Query: 261 PASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSA 320
           P S   G  ++MH++DI +  V  PI++DQ YCPYN+C  ++PS +K+SK+SFKNI+G++
Sbjct: 241 PKS-EPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTS 300

Query: 321 GNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKP 361
               AVKL+CS  +PC  V L+DID+TY+G+ GP  S C N+KP
Sbjct: 301 TTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKP 343

BLAST of CmaCh16G010550 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.8e-114
Identity = 191/362 (52.76%), Postives = 257/362 (70.99%), Query Frame = 0

Query: 3   QSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCK- 62
           QSS  VF+V  YGAK   DI+Q + KAWK ACAS   S ++IPK  Y +G + + GPCK 
Sbjct: 4   QSSGSVFNVNDYGAKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKG 63

Query: 63  SPIDIVVQGTIMAPVD---ITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPK 122
           S I   + G + AP D      DGW+ F  +D ++++G G  DGQGQ AW +N+C KNP 
Sbjct: 64  SKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPN 123

Query: 123 CARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHM 182
           C    M+L+F F+K ++VRDITS +SK FH+N+L C+++TF HVT+ APGTSIN+DGIH+
Sbjct: 124 CKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHV 183

Query: 183 GRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLY 242
           G   GV I N+ I TGDDCIS+G GS+ VTIT V CGPGHGIS+GSLG+Y  E+ V G+ 
Sbjct: 184 GISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGIT 243

Query: 243 VKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKI 302
           VK CT  GT NG+R+KTWP SP  G+ TD+ ++D+ + +VQNP+ILDQEYCPY +C+R+ 
Sbjct: 244 VKGCTFSGTMNGVRVKTWPNSP-PGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQA 303

Query: 303 PSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNV 361
           PS+IK+S ++F NIRG++   VAV + CS   PC ++ + +I+++Y G  GP  S CSNV
Sbjct: 304 PSRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNV 363

BLAST of CmaCh16G010550 vs. ExPASy Swiss-Prot
Match: Q40312 (Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 9.1e-113
Identity = 191/365 (52.33%), Postives = 266/365 (72.88%), Query Frame = 0

Query: 4   SSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSP 63
           +  GV D++ +G KPN+DI Q L  AW +ACAS   +K++IP  TY L  I L GPCK+P
Sbjct: 20  AQSGVLDISKFGGKPNSDIGQALTSAWNEACASTTAAKIVIPAGTYQLNGIELKGPCKAP 79

Query: 64  IDIVVQGTIMAPVD---ITG-DGWIQFRYVDKISLTGGGVFDGQG----QKAWERNDCHK 123
           I++ V GTI AP D   I G + W +F Y+D ++L+G GVFDGQG    +KA   +    
Sbjct: 80  IELQVDGTIQAPADPSVIKGTEQWFKFLYMDHLTLSGKGVFDGQGATVYKKAAPASAWSG 139

Query: 124 NPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDG 183
               +++ M+  F+FV +SIVR +TSKDSKNFHV + GCKN+TFD  TI APG S N+DG
Sbjct: 140 KNSNSKVFMNFGFNFVNNSIVRGVTSKDSKNFHVMVFGCKNITFDGFTITAPGDSPNTDG 199

Query: 184 IHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVA 243
           IHMG+   V I+N+ IGTGDDC+S+GDGS+++T+  V CGPGHG+SVGSLG++  EE V 
Sbjct: 200 IHMGKSTDVKILNTNIGTGDDCVSIGDGSKQITVQGVNCGPGHGLSVGSLGKFTTEENVE 259

Query: 244 GLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCN 303
           G+ VKNCTL  T NG+RIKTWP +P T +V+D+H+EDI + +V+NP+I+DQEY P+N+C+
Sbjct: 260 GITVKNCTLTATDNGVRIKTWPDAPGTITVSDIHFEDITMTNVKNPVIIDQEYYPWNQCS 319

Query: 304 RKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSEC 361
           +K PSKIK+SK+SFKN++G++G A  V L+CS+  PC+ V L+++D+ + G   P  ++C
Sbjct: 320 KKNPSKIKLSKISFKNVKGTSGTAEGVVLICSSAVPCDGVELNNVDLKFNG--APTTAKC 379

BLAST of CmaCh16G010550 vs. ExPASy Swiss-Prot
Match: P35337 (Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.5e-111
Identity = 181/354 (51.13%), Postives = 253/354 (71.47%), Query Frame = 0

Query: 10  DVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQ 69
           +V T G  PN+DIT  + KA+  AC +P  S+++IPK  + LG   + GPCKSPI+  +Q
Sbjct: 23  EVFTAGGPPNSDITAAVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQ 82

Query: 70  GTIMAPVDIT--GDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLK 129
           G +      T   D W+ F  ++   L GGG FDG+G  AW+ N+CHK  +C ++P+S++
Sbjct: 83  GNVKTDGGSTQGKDRWVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVR 142

Query: 130 FSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNII 189
           F FV ++ ++D+TS D+KNFH N++  KN+TFD++ I AP  S N+DGIH+GRC+GV I+
Sbjct: 143 FDFVDNAEIKDVTSLDAKNFHFNVISGKNMTFDNIKIIAPAESPNTDGIHLGRCEGVKIL 202

Query: 190 NSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGT 249
           N+ I TGDDCIS+GDG + + I  V CGPGHGISVGSLG+Y  E+ V  + VKNCTL GT
Sbjct: 203 NTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGSLGRYGWEQDVTDITVKNCTLEGT 262

Query: 250 TNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKV 309
           +NGLRIKTWP++  T +   +H+EDI+L  V NPI++DQEYCP+N+CN+  PS IK+  +
Sbjct: 263 SNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILIDQEYCPWNQCNKNKPSTIKLVDI 322

Query: 310 SFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPR 362
           +F+NIRG++ N  AVKLLCS  +PCE+V + DI+I YTG +GP   EC+NV P+
Sbjct: 323 TFRNIRGTSENKDAVKLLCSKGHPCENVEIGDINIEYTGPDGPPTFECTNVTPK 376

BLAST of CmaCh16G010550 vs. ExPASy Swiss-Prot
Match: Q05967 (Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.8e-108
Identity = 183/361 (50.69%), Postives = 256/361 (70.91%), Query Frame = 0

Query: 3   QSSDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKS 62
           +S  GVFD+T YGA  NADI++ L  A+K+AC S   S ++IPK T+T+  + L GPCKS
Sbjct: 21  ESQTGVFDITKYGANSNADISEALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKS 80

Query: 63  PIDIVVQGTIMAPVD---ITGDGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKC 122
           P+++ +Q T+ AP D   +    W+    +D+ +++GGG+ DGQ   AWE   C ++ KC
Sbjct: 81  PLELQIQATLKAPSDPSQLKVGEWLTVNKLDQFTMSGGGILDGQAAAAWE---CKQSKKC 140

Query: 123 ARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMG 182
            ++P +L F+ + +S ++DIT+ DSK+FHVN+  CKNLTF    ++AP  S N+DGIH+ 
Sbjct: 141 NKLPNNLSFNSLTNSTIKDITTLDSKSFHVNVNQCKNLTFIRFNVSAPANSPNTDGIHVS 200

Query: 183 RCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYV 242
           R   VNI +S   TGDDCIS+GD + ++ IT VTCGPGHGISVGSLG    E+PV G++V
Sbjct: 201 RSSSVNITDSNFSTGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFV 260

Query: 243 KNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIP 302
           +NCT   T NG+RIKTWPAS   G V D+H+EDI++ +V NP+++DQ YCP+NKCN+ +P
Sbjct: 261 RNCTFTNTDNGVRIKTWPAS-HPGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLP 320

Query: 303 SKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVK 361
           S++KISKVSF+NI+G++    AV LL S   PCE + + DIDITY+G+EGP  S C N+K
Sbjct: 321 SQVKISKVSFQNIKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIK 377

BLAST of CmaCh16G010550 vs. TAIR 10
Match: AT5G48140.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 411.4 bits (1056), Expect = 7.7e-115
Identity = 183/345 (53.04%), Postives = 256/345 (74.20%), Query Frame = 0

Query: 18  PNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQGTIMAPVD 77
           P +DIT  L KA+ +AC  P +S ++IPK  Y LG I ++GPCK+PI I + GT+ A  +
Sbjct: 32  PGSDITNALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGN 91

Query: 78  ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLKFSFVKDSIV 137
             G + W+ FR ++   L GGGVFDG+G  AW  N+CHK   C ++P+S++F FV D+ +
Sbjct: 92  ANGKEKWVAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPISIRFDFVTDAKI 151

Query: 138 RDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNIINSVIGTGDD 197
           R ITS D+K+FH+N++G KN+TF+ V I AP  S N+DGIH+GR DG+ IINS I TGDD
Sbjct: 152 RGITSLDAKHFHINVIGAKNVTFEDVKIIAPAESPNTDGIHVGRSDGIKIINSFISTGDD 211

Query: 198 CISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGTTNGLRIKTW 257
           C+S+GDG + + +  VTCGPGHGIS+GSLG+Y  EE V+G+ + NCTL  T NGLRIKTW
Sbjct: 212 CVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNGLRIKTW 271

Query: 258 PASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKVSFKNIRGSA 317
           P++  T + +D+H+E+I+L +V NPI++DQEYCP+N+CN++ PS IK++ +SFK IRG++
Sbjct: 272 PSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFKKIRGTS 331

Query: 318 GNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPR 362
           GN  AVKLLCS  YPC++V + D++I YTG +GP   +CSNV P+
Sbjct: 332 GNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPK 376

BLAST of CmaCh16G010550 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 1.3e-114
Identity = 189/354 (53.39%), Postives = 259/354 (73.16%), Query Frame = 0

Query: 10  DVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQ 69
           +V T G+   +DITQ L KA+  AC S   SK++IPK  + LG I + GPCK+PI++ +Q
Sbjct: 23  EVFTIGSSSGSDITQALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQ 82

Query: 70  GTIMAPVD-ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLK 129
           GT+ A  + I G + W+ F  +D   L GGG FDG+G  AW  N+CHK  +C ++P+S++
Sbjct: 83  GTVKADGNAIQGKEKWVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIR 142

Query: 130 FSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNII 189
           F F+ +S +RDI+S D+KNFH+N+LG KN+T +++ I AP  S N+DGIH+GR DGV I+
Sbjct: 143 FDFILNSEIRDISSIDAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKIL 202

Query: 190 NSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGT 249
           NS I TGDDCIS+GDG + + +  VTCGPGHGISVGSLG+Y  E+ V+G+ V NCTL  T
Sbjct: 203 NSFISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQET 262

Query: 250 TNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKV 309
            NGLRIKTWP++  + + +D+H+EDI+L DV NPI++DQEYCP+N+CN++  S IK+  +
Sbjct: 263 DNGLRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNI 322

Query: 310 SFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPR 362
           SFKNIRG++GN  AVKLLCS  YPC++V + DIDI Y G +GP    CSNV P+
Sbjct: 323 SFKNIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPK 376

BLAST of CmaCh16G010550 vs. TAIR 10
Match: AT3G07840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 401.7 bits (1031), Expect = 6.1e-112
Identity = 183/354 (51.69%), Postives = 259/354 (73.16%), Query Frame = 0

Query: 10  DVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPIDIVVQ 69
           +V   G  P +DITQ L KA+  AC +P  SK++I K  + LG I + GPCK+P++I +Q
Sbjct: 24  EVFNVGGSPGSDITQALLKAFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQ 83

Query: 70  GTIMAP-VDITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARIPMSLK 129
           GT+ A    I G + W+ F  ++   L GGG+FDG+G  AW  N+CHK  +C ++P+S++
Sbjct: 84  GTLKADGKAIQGKERWVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIR 143

Query: 130 FSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCDGVNII 189
           F FV+++ +RDI+S D+KNFH+N+LG KN+TFD+V + AP  S N+DGIH+GR +GV I+
Sbjct: 144 FDFVENAEIRDISSIDAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKIL 203

Query: 190 NSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNCTLIGT 249
           NS I TGDDCIS+GDG + + + NV CGPGHGISVGSLG+Y+ E+ V G+ V NCTL GT
Sbjct: 204 NSKIATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGT 263

Query: 250 TNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKIKISKV 309
            NGLRIKTWP++    + + +H+E+I+L +V NPI++DQEYCP+N+CN++ PS IK+  +
Sbjct: 264 DNGLRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDI 323

Query: 310 SFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPR 362
           SFKNIRG++GN  AVKLLCS  +PC +V + +I++ Y G +GP    CSNV P+
Sbjct: 324 SFKNIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPK 377

BLAST of CmaCh16G010550 vs. TAIR 10
Match: AT3G07830.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 401.4 bits (1030), Expect = 7.9e-112
Identity = 185/359 (51.53%), Postives = 262/359 (72.98%), Query Frame = 0

Query: 5   SDGVFDVTTYGAKPNADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGPCKSPI 64
           S    +V    + P +DITQ L +A+  AC SP   K++IPK  + LG I + GPCKSP+
Sbjct: 19  SANAHEVLRLTSSPGSDITQALLRAFTTACQSPTPRKVVIPKGQFKLGEIMMSGPCKSPV 78

Query: 65  DIVVQGTIMAPVD-ITG-DGWIQFRYVDKISLTGGGVFDGQGQKAWERNDCHKNPKCARI 124
           +I + GT++A  + I G + W+ F+ +D   L GGG FDG+G  AW  N+CHK  +C ++
Sbjct: 79  EITLLGTVLADGNSIHGKEKWVVFQRMDGFRLNGGGTFDGEGNAAWRVNNCHKTFECKKL 138

Query: 125 PMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSDGIHMGRCD 184
           P+S++F FV ++ +RDI+S D+KNFH+N++G KN+TFD+V I AP  S N+DGIH+GR  
Sbjct: 139 PISIRFDFVTNAEIRDISSIDAKNFHINVIGAKNMTFDNVKIMAPAESPNTDGIHLGRSV 198

Query: 185 GVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPVAGLYVKNC 244
           GV+IINS I TGDDC+S+GDG   + + NV CGPGHGISVGSLG+Y  E+ V+G+ V NC
Sbjct: 199 GVSIINSRISTGDDCVSVGDGMVNLLVKNVVCGPGHGISVGSLGRYGHEQDVSGIRVINC 258

Query: 245 TLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKCNRKIPSKI 304
           TL  T NGLRIKTWP++  + + +++H+E+I+L +V NPI++DQEYCP+N+CN++  S I
Sbjct: 259 TLQETDNGLRIKTWPSAACSTTASNIHFENIILRNVSNPILIDQEYCPWNQCNKQKSSSI 318

Query: 305 KISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSECSNVKPR 362
           K++ +SF+ IRG++GN  AVKLLCS  YPCE+V + DI+I YTG +GP    CSNV+P+
Sbjct: 319 KLANISFRRIRGTSGNKDAVKLLCSKGYPCENVQVGDINIQYTGADGPATFMCSNVRPK 377

BLAST of CmaCh16G010550 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 351.7 bits (901), Expect = 7.2e-97
Identity = 166/366 (45.36%), Postives = 239/366 (65.30%), Query Frame = 0

Query: 2   AQSSDGVFDVTTYGAKPN--ADITQILDKAWKDACASPIQSKLIIPKETYTLGVITLLGP 61
           A+      DV   GAK +   D +     AWK+ACA+   S + +PK  Y +  +   GP
Sbjct: 64  AKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEACAA--GSTITVPKGEYLVESLEFKGP 123

Query: 62  CKSPIDIVVQGTIMAPVDITGD----GWIQFRYVDKISLTGG-GVFDGQGQKAWERNDCH 121
           CK P+ + + G   AP  +       GWI F  +   +L G   +FDGQG  AW+ NDC 
Sbjct: 124 CKGPVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCA 183

Query: 122 KNPKCARIPMSLKFSFVKDSIVRDITSKDSKNFHVNILGCKNLTFDHVTINAPGTSINSD 181
           K  KC  +P++++F+ + +S +  ITS +SK FH+NIL CKN+T   + I+AP  S+N+D
Sbjct: 184 KTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTD 243

Query: 182 GIHMGRCDGVNIINSVIGTGDDCISLGDGSREVTITNVTCGPGHGISVGSLGQYLVEEPV 241
           GIH+GR +GVN+I + I TGDDC+S+GDG+  + + NV CGPGHGIS+GSLG+Y  E+PV
Sbjct: 244 GIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPV 303

Query: 242 AGLYVKNCTLIGTTNGLRIKTWPASPATGSVTDMHYEDIVLVDVQNPIILDQEYCPYNKC 301
            G+ V+ C +  T NG+RIKTWP SP  G  +++ +EDI + +V  P+++DQEYCPY  C
Sbjct: 304 KGVTVRKCLIKNTDNGVRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHC 363

Query: 302 NRKIPSKIKISKVSFKNIRGSAGNAVAVKLLCSADYPCEDVTLSDIDITYTGREGPVVSE 361
              +PSK+K+S V+ KNI+G++   VAVKL+CS   PC ++ LSDI++ + G+EGP VS 
Sbjct: 364 KAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSA 423

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P245487.7e-12057.56Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
Q6H9K02.8e-11452.76Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q403129.1e-11352.33Polygalacturonase OS=Medicago sativa OX=3879 PE=2 SV=1[more]
P353376.5e-11151.13Polygalacturonase OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q059671.8e-10850.69Polygalacturonase OS=Nicotiana tabacum OX=4097 GN=PG1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT5G48140.17.7e-11553.04Pectin lyase-like superfamily protein [more]
AT3G07820.11.3e-11453.39Pectin lyase-like superfamily protein [more]
AT3G07840.16.1e-11251.69Pectin lyase-like superfamily protein [more]
AT3G07830.17.9e-11251.53Pectin lyase-like superfamily protein [more]
AT3G14040.17.2e-9745.36Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 181..202
e-value: 1600.0
score: 5.9
coord: 234..255
e-value: 29.0
score: 13.6
coord: 299..324
e-value: 9100.0
score: 0.4
coord: 204..224
e-value: 2100.0
score: 4.9
coord: 154..180
e-value: 210.0
score: 10.7
coord: 332..354
e-value: 4700.0
score: 2.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 34..362
e-value: 9.2E-101
score: 337.1
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 211..224
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 1..364
e-value: 4.0E-105
score: 354.1
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 5..360
NoneNo IPR availablePANTHERPTHR31375:SF248POLYGALACTURONASEcoord: 5..360
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 7..360

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G010550.1CmaCh16G010550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity