CmaCh16G010510 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010510
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein, putative
Location	Cma_Chr16: 8053159 .. 8053536 (+)
RNA-Seq Expression	CmaCh16G010510
Synteny	CmaCh16G010510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTATCGCCGCCCTCACTGTCGCTCTCACGCACACGCACGATGCCTCATCGACCATCACGAGCCTGGCCAAATCGAATGCCTTAACACAGCAAGAGAGCTATGATTTGAGTGATTGCATGGAAGAATTTGGAGATTCAATAGAAGAGTTAAAGATGGCTGTGGAGGAGGAGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAGGATATTCAGACGTGGGTAAGCGCTGCTCTGACCGACGACAACAGGTGCATGGACGGGTTCGCCGGAGACACAATGAACGGTAGCGTGAAGGAGTCGGTGAGGACATTGGTGGTGGATGTTGCTCAATTGATAACCATTGCTCTCTCACTTGTTAGGCAACTGAAGTGA

mRNA sequence

ATGACTATCGCCGCCCTCACTGTCGCTCTCACGCACACGCACGATGCCTCATCGACCATCACGAGCCTGGCCAAATCGAATGCCTTAACACAGCAAGAGAGCTATGATTTGAGTGATTGCATGGAAGAATTTGGAGATTCAATAGAAGAGTTAAAGATGGCTGTGGAGGAGGAGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAGGATATTCAGACGTGGGTAAGCGCTGCTCTGACCGACGACAACAGGTGCATGGACGGGTTCGCCGGAGACACAATGAACGGTAGCGTGAAGGAGTCGGTGAGGACATTGGTGGTGGATGTTGCTCAATTGATAACCATTGCTCTCTCACTTGTTAGGCAACTGAAGTGA

Coding sequence (CDS)

ATGACTATCGCCGCCCTCACTGTCGCTCTCACGCACACGCACGATGCCTCATCGACCATCACGAGCCTGGCCAAATCGAATGCCTTAACACAGCAAGAGAGCTATGATTTGAGTGATTGCATGGAAGAATTTGGAGATTCAATAGAAGAGTTAAAGATGGCTGTGGAGGAGGAGTTGAAGGGTAATGACAAAACCCGACTTGACACTGAGGATATTCAGACGTGGGTAAGCGCTGCTCTGACCGACGACAACAGGTGCATGGACGGGTTCGCCGGAGACACAATGAACGGTAGCGTGAAGGAGTCGGTGAGGACATTGGTGGTGGATGTTGCTCAATTGATAACCATTGCTCTCTCACTTGTTAGGCAACTGAAGTGA

Protein sequence

MTIAALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEEELKGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALSLVRQLK

Homology

[Close]

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.3e-15
Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE-----EL 64
           AL V+LT T  A + +  L K   L  ++   + DC+EE  DS++ +  + +E       
Sbjct: 64  ALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHA 123

Query: 65  KGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALS 122
           KGND T     +++TWVSAALTD+  CMDGFAG  M+G +KESVR  VV VA++ + AL+
Sbjct: 124 KGNDFT-FRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALA 183

[Close]

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-12
Identity = 46/125 (36.80%), Postives = 77/125 (61.60%), Query Frame = 0


Query: 1   MTIAALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE--- 60
           + + AL + L+    AS  + +++    LT+ E   ++DC+EE GDS+  L+ ++ E   
Sbjct: 179 LAVIALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDS 238

Query: 61  -ELKGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITI 120
              K + K  +   D++TWVSAALT+D+ CMDGF+   +  +VK+ VR  VV+VA+L + 
Sbjct: 239 INYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFS--LVKTAVKDLVRRHVVEVARLTSN 298

Query: 121 ALSLV 122
           AL+L+
Sbjct: 299 ALALI 301

[Close]

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 9.1e-12
Identity = 43/122 (35.25%), Postives = 68/122 (55.74%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE-----EL 64
           AL V +T        +  L +  +L ++E   + DC+EE  D+++ L M++ E       
Sbjct: 74  ALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSA 133

Query: 65  KGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALS 122
           KG D+      + QTW SAALT+ N C DGFAG  M+G VK SVR  ++++ +  + AL+
Sbjct: 134 KGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALA 193

[Close]

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 69.7 bits (169), Expect = 2.6e-11
Identity = 39/122 (31.97%), Postives = 70/122 (57.38%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVE-----EEL 64
           AL V++     A + ++ +     +T+++   ++DC+EE GD+++ L  +++     EE 
Sbjct: 73  ALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEG 132

Query: 65  KGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALS 122
              +       +++TW SAALTD+  CMDGF G  M G +K  +RT +V VA+  + AL+
Sbjct: 133 DSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALA 192

[Close]

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.8e-10
Identity = 47/145 (32.41%), Postives = 77/145 (53.10%), Query Frame = 0


Query: 2   TIAALTVALTHTHD----ASSTITSLAKSNALT--------------QQESYDLSDCMEE 61
           T++A    + H +D     ++ I SLA++ ++T              ++E   + DC+E 
Sbjct: 50  TLSAYATKIRHNNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEV 109

Query: 62  FGDSIEELKMAVEEELKGNDKTRLD-----TEDIQTWVSAALTDDNRCMDGFAGDTMNGS 121
            G+S++ L  +V+E  +       +       ++QTWVSAALTD+  C+DGF+   M G 
Sbjct: 110 LGNSVDRLAQSVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGK 169

Query: 122 VKESVRTLVVDVAQLITIALSLVRQ 124
           VK  +R  VV VAQ+ + AL+LV Q
Sbjct: 170 VKRLIRYKVVHVAQVTSNALALVNQ 194

[Close]

BLAST of CmaCh16G010510 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 74.7 bits (182), Expect = 5.8e-14
Identity = 51/123 (41.46%), Postives = 75/123 (60.98%), Query Frame = 0


Query: 1   MTIAALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE--E 60
           M  AAL + L      S+ +  L+ S  L  +E   + DC+EE GD++EEL+ ++ E  +
Sbjct: 75  MAHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQ 134

Query: 61  LKGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIAL 120
           L G++   +   DIQTWVSAALTD N C DGF G+ M+G VK  VR  ++ +A L + AL
Sbjct: 135 LSGSN-YEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNAL 194

Query: 121 SLV 122
           +L+
Sbjct: 195 ALI 195

[Close]

BLAST of CmaCh16G010510 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.0e-13
Identity = 46/125 (36.80%), Postives = 77/125 (61.60%), Query Frame = 0


Query: 1   MTIAALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE--- 60
           + + AL + L+    AS  + +++    LT+ E   ++DC+EE GDS+  L+ ++ E   
Sbjct: 179 LAVIALNLTLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDS 238

Query: 61  -ELKGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITI 120
              K + K  +   D++TWVSAALT+D+ CMDGF+   +  +VK+ VR  VV+VA+L + 
Sbjct: 239 INYKDSAKFEMVMSDVETWVSAALTNDDTCMDGFS--LVKTAVKDLVRRHVVEVARLTSN 298

Query: 121 ALSLV 122
           AL+L+
Sbjct: 299 ALALI 301

[Close]

BLAST of CmaCh16G010510 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.2e-13
Identity = 42/122 (34.43%), Postives = 67/122 (54.92%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE-----EL 64
           A+ V L+        ++ L +   L ++E   + DC+EE  D+++ L  +V+E       
Sbjct: 74  AIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSA 133

Query: 65  KGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALS 122
           K  D+      + QTW SAALTD+N C DGF+G  M+G +K SVR  +++V    + ALS
Sbjct: 134 KDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALS 193

[Close]

BLAST of CmaCh16G010510 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 71.6 bits (174), Expect = 4.9e-13
Identity = 45/126 (35.71%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEEELKGNDK 64
           AL V L      +  +  L K+  + ++E   + DC+E  GD +E L  ++  ELK   +
Sbjct: 75  ALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSM-RELKQVGR 134

Query: 65  TRLDTED-------IQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIA 124
           +  D ++       ++TWVSAALTD+  C+DGF G  M+G VK ++R  VV VA++ + A
Sbjct: 135 SGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVARVTSNA 194

[Close]

BLAST of CmaCh16G010510 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 71.2 bits (173), Expect = 6.5e-13
Identity = 43/122 (35.25%), Postives = 68/122 (55.74%), Query Frame = 0


Query: 5   ALTVALTHTHDASSTITSLAKSNALTQQESYDLSDCMEEFGDSIEELKMAVEE-----EL 64
           AL V +T        +  L +  +L ++E   + DC+EE  D+++ L M++ E       
Sbjct: 74  ALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSA 133

Query: 65  KGNDKTRLDTEDIQTWVSAALTDDNRCMDGFAGDTMNGSVKESVRTLVVDVAQLITIALS 122
           KG D+      + QTW SAALT+ N C DGFAG  M+G VK SVR  ++++ +  + AL+
Sbjct: 134 KGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALA 193

The following BLAST results are available for this feature:

BLAST of CmaCh16G010510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	2.3e-15	43.44	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	1.4e-12	36.80	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	9.1e-12	35.25	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SB37	2.6e-11	31.97	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI72	3.8e-10	32.41	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of CmaCh16G010510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	5.8e-14	41.46	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	1.0e-13	36.80	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	2.2e-13	34.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G12390.1	4.9e-13	35.71	pectin methylesterase inhibitor 1	[more]
AT3G47380.1	6.5e-13	35.25	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 1..120 e-value: 1.5E-14 score: 64.4
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 5..124 e-value: 5.0E-18 score: 63.0
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 4..120 e-value: 7.7E-21 score: 75.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 1..125 e-value: 1.8E-23 score: 85.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 3..124
None	No IPR available	PANTHER	PTHR31080:SF96	21 KDA PROTEIN-LIKE	coord: 3..122
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 3..122
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 3..121 e-value: 3.54857E-23 score: 85.9529

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010510.1	CmaCh16G010510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity