CmaCh16G010440 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010440
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Calcium-binding EF-hand family protein
Location	Cma_Chr16: 8024498 .. 8024941 (-)
RNA-Seq Expression	CmaCh16G010440
Synteny	CmaCh16G010440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGATCTGAGCGATGAGCAGAAATCCTCCATGAAAGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGACGGATCGCTCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATAGCTGAGGAGAACCTCACTGCTTCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCTTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

mRNA sequence

ATGGGCAAAGATCTGAGCGATGAGCAGAAATCCTCCATGAAAGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGACGGATCGCTCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATAGCTGAGGAGAACCTCACTGCTTCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCTTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGATGAGCAGAAATCCTCCATGAAAGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGACGGATCGCTCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATAGCTGAGGAGAACCTCACTGCTTCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCTTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Protein sequence

MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGPDGKIRYEDFIARMVAK

Homology

[Close]

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 2.9e-65
Identity = 123/148 (83.11%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSD+Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  ASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           + FDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.0e-62
Identity = 118/148 (79.73%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS++Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  ASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           + FDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 1.7e-57
Identity = 107/146 (73.29%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 2   GKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAS 61
           GK+LS+EQ +SM+EAF+LFD+DGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LTA 
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++ EPFDR LRDAF+VLDK+ +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGPDGKIRYEDFIARMVAK 148
           EWIREV+V PDG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

[Close]

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot
Match: P84339 (Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 1.5e-34
Identity = 72/149 (48.32%), Postives = 103/149 (69.13%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   LS+EQ S  KEAF+LFD DGDG I   ELG +MRSLG NP+QA+L+ +I E +   
Sbjct: 1   MADQLSEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPSQAELEDMINEVDADG 60

Query: 61  TASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
             + DF  FL +M++ M+    + ++++AFKV DK+  GY+  +ELRH++T++GEKL  S
Sbjct: 61  NGTIDFPEFLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDS 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M++K
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKMMLSK 149

[Close]

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot
Match: P13868 (Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 2.0e-34
Identity = 69/149 (46.31%), Postives = 104/149 (69.80%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M + L++EQ +  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I+E +   
Sbjct: 1   MAEQLTEEQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMISEADADQ 60

Query: 61  TASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
             + DF  FL +M++ MK    + +L++AFKV DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFKVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE ++  DG++ YE+F+  M+AK
Sbjct: 121 EVDEMIREADIDGDGQVNYEEFVRMMLAK 149

[Close]

BLAST of CmaCh16G010440 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 249.2 bits (635), Expect = 2.0e-66
Identity = 123/148 (83.11%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LSD+Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  ASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           + FDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of CmaCh16G010440 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 240.7 bits (613), Expect = 7.2e-64
Identity = 118/148 (79.73%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LS++Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  ASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           + FDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of CmaCh16G010440 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 145.6 bits (366), Expect = 3.2e-35
Identity = 72/149 (48.32%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DEQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
             + DF  FL +M+K MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E +E IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVEEMIREADVDGDGQINYEEFVKIMMAK 149

[Close]

BLAST of CmaCh16G010440 vs. TAIR 10
Match: AT5G37780.1 (calmodulin 1 )

HSP 1 Score: 145.6 bits (366), Expect = 3.2e-35
Identity = 72/149 (48.32%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+DEQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
             + DF  FL +M+K MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E +E IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVEEMIREADVDGDGQINYEEFVKIMMAK 149

[Close]

BLAST of CmaCh16G010440 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 145.2 bits (365), Expect = 4.1e-35
Identity = 71/149 (47.65%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L+D+Q S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TASFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
             + DF  FL +M++ MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of CmaCh16G010440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	2.9e-65	83.11	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	1.0e-62	79.73	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	1.7e-57	73.29	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P84339	1.5e-34	48.32	Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2	[more]
P13868	2.0e-34	46.31	Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2	[more]

BLAST of CmaCh16G010440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	2.0e-66	83.11	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	7.2e-64	79.73	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	3.2e-35	48.32	calmodulin 4	[more]
AT5G37780.1	3.2e-35	48.32	calmodulin 1	[more]
AT5G21274.1	4.1e-35	47.65	calmodulin 6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 119..147 e-value: 6.9 score: 11.0 coord: 12..40 e-value: 3.0E-4 score: 30.1 coord: 83..111 e-value: 0.013 score: 24.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 3.7E-12 score: 46.5
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 9.3E-6 score: 25.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 11.082272
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.811786
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..72 e-value: 5.98039E-9 score: 47.5425
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 3.5E-21 score: 77.2
None	No IPR available	GENE3D	1.10.238.10		coord: 81..145 e-value: 5.4E-18 score: 66.9
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010440.1	CmaCh16G010440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding