CmaCh16G010380 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G010380
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	methylsterol monooxygenase 1-2
Location	Cma_Chr16: 7975567 .. 7980543 (+)
RNA-Seq Expression	CmaCh16G010380
Synteny	CmaCh16G010380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCACGCTCTTCCTCTCCTTTCCTTTCAAAATTCCCGCATTATTTTAACTGTTTCTCGATCCTCCGCCCCTTTCCGCCGCTCCTCAATCCATTCACCGCCGCCCACCCTCTCTCCGCCGGATTCCTCCACCCAATGTTGCCCTTCCACTCCATCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGATCTTGCACGCCTCCGGGATTCATAAGTTCAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTCGTTGGCCCTCTTCAACTTGTCTCATTCCCTTCCATTCAGGTGAATCTCGGGTTTCGATTTCGCGATTTCTACCCTCTTGTTGATTTAGGATTTTTATTTTGAGATTGTTTTTCGGTTTCTTTTGGATCATTAGATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGAATTCTAAAGCTATATCTCTCACACACTGTGTTAAAAGTACTTTTAATCTGAAGGATCTTGAGTGGTGGGCTTACCTCTTTTTTGACTACGACCGAGATCTGCTCTATTGCTTTCTTCTTTTTTAATCTTGATTTCCTTTTCCTCATTATATATATATATATTTTATCTATATAGATTTCCATCATTACTGTATGAATTTATTGCATGCTGAATGATTGATCCTACTAACTTGATATAACGAAGTTTGAAGTTTTGTGTTGAGAAATATTGAAAGGGTTAAATTTACCATTCTTTGAGTTGTCTAGCATCAACTTTTTGAGCTTCCGCATTCTTCATACAGCTGTGTGAAAGCTCGATATTCCTTTGAATTTATCGTAAGGGGGTGTATAAATCTTCTATGAGAAGCTTAGAAGCTTGATTCTTATATACTAAACTTATTATAGGGCCCACTACCCCTTTATGCTTTAAACTTATGGATATCATTTCAAGATCCATGATTTAACATGCTAGAAGTAGCTAGTCTTGAGTATTGTTATTTTTTTCTTCTCAATTTTGTATTAGGCTTTGTGTGCTAACACTTGACTTTACTTTCTCCGATTAGTGGGACCGTAGATACATTAGACTATAGTTTTTAGTGAAGAATGAAATTGGCGTATGGGGAAACGAAAAACATCAAAGTGGTTGTGTCAATGTGTTTGCATCTATGGTAGGTAAGTACAATGAAACAATGTTGTTGTGACGTGATGTTGCAATGATATCGATGGGCAAAGGAAAAGATCACGAACAGGATTATAACCGAACCTTCTTGGTTCCAATATTCTTCGCTTCTGGGGAACCTTTAGCTCACGACCATTCCCGATCTTCCTGATCTTACTCCATTGTGGTTGTTTATGGGGCTTCACCTTTTCCAACTCAATGTTAAAATTTCATAGCCTTGGGATTGTTGGGAAATGAAAGGAAAAGAACAATTAGCTGATGTGTAGAAAGACATCTTTAGAGAATTGCTTCTAGCCCGATGGAATAGACATATAGGGGTGCTCACGGGTTAGACTCGTGTAACTTGGGTTAAGAAGCCAAACATATTCTAGCATATGGTTTGCTTATTTTGAGCTGAGTTTGTTAATTCCATGGGTTACATAATCCCACTGGTTATACAAATCCAAGTTTCCATAACTGTGAGATCTCACATCGGTTGGAGAGAGGAACGAAGCATTCCTTAAGAGGGTGTGGATAGGACAATATCTACTAGCGGTGGGCTTGAGCTGTTACAATAACCTACCTATCCCTCAGTACGTTCTAAACACAAATAAATTATATAAACTCAGCTTTTCAATAAACCAACCTTATTAAATTGTATCTTCCTCTTCCTCCTCACAAGAATCAAAAGCAAGCTCTGTTATTCATGTTGGCTCATTCGTTTTAGAGGAGTATAGAGGTAAACAAAAGTAAATTGACATCTCAAGGGGCCAGCTTACTCATATAAGAATCCATTGATTTGACCTCCAAATTGTTTAGTTGGCCGGAAACGAAAATAATTGATGTCAGGCTTATGGCTGGGAACAAATGCGGTGTCAGACAATCATTTCTTTGGTCATGTAGGACATAGCTTCACTCTTGGTGGGTTTCCTGTGGTTGAACAGCCCTTTAGCCCTTGGCTGAACTCGAGTAACTTTGTGTTATTATTTTATATTGCTGAAGTTTAATTGTTTTGTCTCCTTCACACAAAGATTGAGATTCGTATGCACATTGATGGAAAGTTGTTGTATAACTTTTGATGCTAACGAGAATGTCGAAAAAAATTAAAAAATGAAGAAATTCACAACAGTAATCTGAAGTTGAACTATTGACCTCTTCGTTTGTAGTTTTAGAAGTTTAATTATCATCTTTCATCACTGATTGTTAGTAGTTTTCTTATGATTTCTATGAATGTGAATGAGAGGAGTAAAGGTTTAATGGGTAAGCCTTACTGCATATCTCCAAAATCCACTGTTTTCTCTCTTTACCATGTCCACTTAATACTTGTGAGATCCCACGTTGATTGGAGAGGGGTACGAAACATTATTTATTAAAGGTGTGGAAACCTCAAACCTCTTCCTAGAAGACGTGTTCTAAATCCGTGAGGGGATGCTCGGAAGGGAAAGTCCAAAGATGACAATATCTTCTAGCGATGGGCTTGGGTTGTTACACTACTGGAGATGTTCAGGGAGGTAGTCCCAAACTAGAGGGACTATAATAAACTTTTAAAACTATAGAGATCAAGTTGAAGTCCCGTGCAAACCATAGAGACCAAAATCGTAATACGATCGTACTTTGTTACAAATAAACTACTCGTGAGATCCCACATCGATTGAATAGAGGAACGATTGCATTCTTTATAAAGGTGTGGAACTAGCAAACGTGTTTTAAAAACCTTGAGGAGAAAACCCAAAGAAGATAATATCTACTAGCGGTGGGTTAGGACCTTTATAAATGGTATCAAAGCCAGATTCTGGATGGTGTGCCAACGAAGATGTTGACCTCGAAGAGGATGAATTGTGAGATCCCACATCGTTGGAAGAGAGGAATGATTGTATTCTTTATAAGGGTGTGAAAATCTTTCCTAGTAGACACGTTTTAAAAACCTTAAGGAGAAGCTCGAAAGAAAAAACCCAAAAAAGATAATATCTACTAGTGGTAGGCGGGTGCCTTTACACTATTCGTGAGAACTATATCATTTCTCTTCCTCTTTAGAGTGTTGAGATCATCCCAGGTTTTATTTATACATATTATATTCATATTCAGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGTAGTTATGCTGCTCTTCTTATAATTTTACCTCCCACTTCTTCTTCCAGTAAAACCATGCTCATAAAAGTTACTCATTTGTTTACTTCCTCAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGGTAAGCATTTTGTTTTCGCCCCCGCCCCCACCTTCACCCCGAAAATTGATTGTATATGGTGTTGGGAACTTGCATTGGTTTCATGTTTTCTCCAACTGGACATTATATATACCCATGTTTGAGCTTGGCTCGATAGCGTGGCCTTGGGATTGTTAGTTTAACTATATTTGGTTGGATCAAATGTGAGATCCGACGTTGGTGGAAGAGGGGAATGGAGCATTCCTTATTGAGTGTGAAAACCTCTCCCTAACAAACGTGTTTTAAAACTGTGATGTTGGCGACGATATGTAGCGGGTTAAAATGGACAATATTTGCTAACGGTGGGTTTGAGCTGTTACAAATGGTATCAAAGCCAGACTTCGGGCGGTGTCCCAATGAGGTCGCTGGGTCCCTAAGGGGTAGATTGTGAGATCCCATGTCGGTTAGAAAAGGGAACAAAACATTCCTTATAGTACGTGGAAACTTCTCCCTAATAGACACGTTTGATAACCATGAGGTTAACGGGATACGTAAGGGGCTAAAACGGACAATATCTGTTATCTGTAGGCCGCTTGGGATGTTACATCAAATCTATTTCATTTGTTCTTTTTTAACGGGCTACACCGCACCTGTTAAATCATTGTGTTCTAATTGTTTGTTGGTGGTGGAATCAGTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAGACGATGAATCGGGAACGGGGGATATTCATGTTTTGAAGGATGGGGTTTTTTTTTCTTTTCGTGTCGGTAGCAACGTTACGTGATCTGTGATTGGACGTTACGTGGTCTCATCGACGAGGTAGTTAGGGAGTGGGATGGGAAGGAAATTAACATGGGTCTATAAAAAAAGTTTAATTGTTGTTAGCTTAATATGTTGAGGCAGAGAAGTCGAATTCTGCGGTGGCTTTGTGTGTGGGTTTAACCTTATTGTGCAGACAAAACTGGTAATATAAATATGTATTCTGGTTGTGAAATTTAATGCTTTTATTGGGCTA

mRNA sequence

TTCACGCTCTTCCTCTCCTTTCCTTTCAAAATTCCCGCATTATTTTAACTGTTTCTCGATCCTCCGCCCCTTTCCGCCGCTCCTCAATCCATTCACCGCCGCCCACCCTCTCTCCGCCGGATTCCTCCACCCAATGTTGCCCTTCCACTCCATCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGATCTTGCACGCCTCCGGGATTCATAAGTTCAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTCGTTGGCCCTCTTCAACTTGTCTCATTCCCTTCCATTCAGATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAGACGATGAATCGGGAACGGGGGATATTCATGTTTTGAAGGATGGGGTTTTTTTTTCTTTTCGTGTCGGTAGCAACGTTACGTGATCTGTGATTGGACGTTACGTGGTCTCATCGACGAGGTAGTTAGGGAGTGGGATGGGAAGGAAATTAACATGGGTCTATAAAAAAAGTTTAATTGTTGTTAGCTTAATATGTTGAGGCAGAGAAGTCGAATTCTGCGGTGGCTTTGTGTGTGGGTTTAACCTTATTGTGCAGACAAAACTGGTAATATAAATATGTATTCTGGTTGTGAAATTTAATGCTTTTATTGGGCTA

Coding sequence (CDS)

ATGTTGCCCTTCCACTCCATCTCCGCCGCCCAGCTTGCGCTTGGCCGCAATCTCACCTTCTTCGAGACCCTCTGGTTCAACTACTCCCATGACAAGTCCGATTACTTCCTCTTTTGCCATAACATTCTCTTCCTGTTCTTGATTTTCTCCTTTATTCCCCTTCCCCTTATCTTCCTTGAGATCTTGCACGCCTCCGGGATTCATAAGTTCAAAATTCAGCCCAAAGTCCGGTTGCCATTTTCCGAGATCTTCCGCTGCTACAAGGATGTTATGCGCATGTTCTTCCTTGTCGTTGGCCCTCTTCAACTTGTCTCATTCCCTTCCATTCAGATGATTGGAATCAGGACGGGTTTGCCATTGCCTTCTGGTTGGGAAATTGTTTCTCAATTGGTGGTGTATTTCATGGTGGAGGATTATACCAATTATTGGATTCATAGATTCCTCCATTGCAAATGGGGGTATGAGAATATCCATCAAGTTCATCATGAATACACTGCTCCTATTGGATTTGCAGCTCCTTACGCTCATTGGGCGGAGGTTTTGATCCTTGGAATCCCCTCTTTTCTTGGTCCTGCTATGGTTCCTGGTCATATGATCACCTTCTGGTTGTGGATTGCGCTAAGGCAGATTGAGGCCATTGACACGCATAGTGGGTATGATTTCCCCTGGAGTCTAACGAAATTCATACCATTTTACGGTGGTGCGGACCATCATGATTACCATCACTATGTTGGAGGACAAAGCCATAGCAACTTTGCTTCAGTATTTACCTACTGTGATTATATTTACGGAACTGACAAGGGGTATCGCTATCAAAAGAAGATCCTTAAGAAGTTGAAGGAAGAAGTGAAAAGCAGTGAAGGATCATACTACAGCACAGCCGAAGATGTTAAAGTGGACTAG

Protein sequence

MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLEILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSSEGSYYSTAEDVKVD

Homology

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BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot
Match: Q1EC69 (Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 6.5e-133
Identity = 217/288 (75.35%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ +I  A +AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHASG--IHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
              ++    +++KIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYE  H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE+ K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

[Close]

BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot
Match: Q8L7W5 (Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 1.5e-129
Identity = 212/288 (73.61%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-ASGI-HKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
           +   ASG+ +++KIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+ IH+VHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE  K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKS 288

[Close]

BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot
Match: F4JLZ6 (Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.1e-119
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   +FKIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYE IH++HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEVK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

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BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot
Match: Q9AV39 (Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-10 PE=2 SV=1)

HSP 1 Score: 422.5 bits (1085), Expect = 3.9e-117
Identity = 194/280 (69.29%), Postives = 235/280 (83.93%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           MLP+ + + A+ ALGR +T  E+LWF YS    DY LF HNILFLF++F+  PLP+  LE
Sbjct: 1   MLPYATAAEAEAALGRAMTAAESLWFRYSAGIPDYVLFWHNILFLFVVFTLAPLPVALLE 60

Query: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
           +   + +  FK+QPKVRL   E FRCY+DVMR+FFLV+GPLQLVS+P+++M+GI TGLPL
Sbjct: 61  LRAPAAVGPFKLQPKVRLSREEFFRCYRDVMRLFFLVIGPLQLVSYPTVKMVGIHTGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180
           PS  E+ +QL+VYF+VEDY NYWIHR LH +WGYE IH+VHHE+TAPIGFAAPYAHWAEV
Sbjct: 121 PSLGEMAAQLLVYFLVEDYLNYWIHRLLHGEWGYEKIHRVHHEFTAPIGFAAPYAHWAEV 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIPSF+GPA+ PGHMITFWLWI LRQ+EAI+THSG+DFP++LTK+IPFYGGA++HDY
Sbjct: 181 LILGIPSFVGPALAPGHMITFWLWIVLRQMEAIETHSGFDFPFNLTKYIPFYGGAEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLK 281
           HHYVG QS SNFASVFTYCDY+YGTDKGYRY K    K+K
Sbjct: 241 HHYVGRQSQSNFASVFTYCDYLYGTDKGYRYHKAYQAKMK 280

[Close]

BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.6e-57
Identity = 117/264 (44.32%), Postives = 159/264 (60.23%), Query Frame = 0


Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKFKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + K+KIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEIVSQLVVYFMVE 138
            P ++  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKKLKE 282
           Y D+I+GTDKGYR     LK LKE
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKE 259

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BLAST of CmaCh16G010380 vs. TAIR 10
Match: AT4G22756.1 (sterol C4-methyl oxidase 1-2 )

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-134
Identity = 217/288 (75.35%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ +I  A +AL RNLT+ ETLWF+YS  KSDY+L+CHNILFLFLIFS +PLPL+F+E
Sbjct: 1   MIPYATIEEASIALSRNLTWLETLWFDYSATKSDYYLYCHNILFLFLIFSLVPLPLVFIE 60

Query: 61  ILHASG--IHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
              ++    +++KIQPKV+  FS +F+CYKDVM+MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  SSQSTSDLFNRYKIQPKVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWA 180
           PLPS  EIV+QLVVYF+VEDYTNYW+HRF HCKWGYE  H +HHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPSCMEIVAQLVVYFLVEDYTNYWVHRFFHCKWGYEKFHHIHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+FLGPA+ PGHMITFWLWIALRQIEAI+THSGYDFPWSLTK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFLGPAIAPGHMITFWLWIALRQIEAIETHSGYDFPWSLTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE+ K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLQQMKEKSKKS 288

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BLAST of CmaCh16G010380 vs. TAIR 10
Match: AT4G12110.1 (sterol-4alpha-methyl oxidase 1-1 )

HSP 1 Score: 463.8 bits (1192), Expect = 1.1e-130
Identity = 212/288 (73.61%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT  ETLWF+YS  KSDY+L+CHNILFLFL+FS +PLPL+F+E
Sbjct: 1   MIPYATVEEASIALGRNLTRLETLWFDYSATKSDYYLYCHNILFLFLVFSLVPLPLVFVE 60

Query: 61  ILH-ASGI-HKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGL 120
           +   ASG+ +++KIQPKV    S++F+CYKDVM MF LVVGPLQLVS+PSIQMI IR+GL
Sbjct: 61  LARSASGLFNRYKIQPKVNYSLSDMFKCYKDVMTMFILVVGPLQLVSYPSIQMIEIRSGL 120

Query: 121 PLPSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWA 180
           PLP+  E++SQLVVYF++EDYTNYW+HRF H KWGY+ IH+VHHEYTAPIG+AAPYAHWA
Sbjct: 121 PLPTITEMLSQLVVYFLIEDYTNYWVHRFFHSKWGYDKIHRVHHEYTAPIGYAAPYAHWA 180

Query: 181 EVLILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHH 240
           EVL+LGIP+F+GPA+ PGHMITFWLWIALRQ+EAI+THSGYDFPWS TK+IPFYGGA++H
Sbjct: 181 EVLLLGIPTFMGPAIAPGHMITFWLWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYH 240

Query: 241 DYHHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKILKKLKEEVKSS 287
           DYHHYVGGQS SNFASVFTYCDYIYGTDKGYR+QKK+L+++KE  K S
Sbjct: 241 DYHHYVGGQSQSNFASVFTYCDYIYGTDKGYRFQKKLLEQIKESSKKS 288

[Close]

BLAST of CmaCh16G010380 vs. TAIR 10
Match: AT4G22753.1 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 431.0 bits (1107), Expect = 7.7e-121
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   +FKIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYE IH++HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEVK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

[Close]

BLAST of CmaCh16G010380 vs. TAIR 10
Match: AT4G22753.2 (sterol 4-alpha methyl oxidase 1-3 )

HSP 1 Score: 431.0 bits (1107), Expect = 7.7e-121
Identity = 194/285 (68.07%), Postives = 245/285 (85.96%), Query Frame = 0


Query: 1   MLPFHSISAAQLALGRNLTFFETLWFNYSHDKSDYFLFCHNILFLFLIFSFIPLPLIFLE 60
           M+P+ ++  A +ALGRNLT+FET+WF+YS  KS++ ++CH IL LFL+FS  P PL+ +E
Sbjct: 1   MIPYPTVEDASVALGRNLTWFETVWFDYSATKSNFHVYCHTILVLFLVFSLAPFPLVIVE 60

Query: 61  ILHASGIHKFKIQPKVRLPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPL 120
                   +FKIQ KV+   S++F+CYK+VM++F LVVG LQ+VS+PSIQM+GIR+GLPL
Sbjct: 61  --WTGWFDQFKIQKKVKYSLSDMFQCYKEVMKLFLLVVGTLQIVSYPSIQMVGIRSGLPL 120

Query: 121 PSGWEIVSQLVVYFMVEDYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEV 180
           PS  EIV+QLVVYF++EDYTNYWIHR++HCKWGYE IH++HHEYT+PIG+A+PYAHWAE+
Sbjct: 121 PSLMEIVAQLVVYFLIEDYTNYWIHRWMHCKWGYEKIHRIHHEYTSPIGYASPYAHWAEI 180

Query: 181 LILGIPSFLGPAMVPGHMITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDY 240
           LILGIP+FLGPA+ PGH++TFWLWI+LRQ EAI+THSGYDFPWS+TK IPFYGG ++HDY
Sbjct: 181 LILGIPTFLGPAIAPGHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDY 240

Query: 241 HHYVGGQSHSNFASVFTYCDYIYGTDKGYRYQKKIL-KKLKEEVK 285
           HHYVGGQS SNFASVFTYCDYIYGTDKGYR  KK+L  ++KEE +
Sbjct: 241 HHYVGGQSQSNFASVFTYCDYIYGTDKGYRIHKKLLHHQIKEEAE 283

[Close]

BLAST of CmaCh16G010380 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 223.8 bits (569), Expect = 1.9e-58
Identity = 117/264 (44.32%), Postives = 159/264 (60.23%), Query Frame = 0


Query: 19  TFFETLWFNYSHDKSDYFLFCHNILFLF-LIFSFIPLPLIFLEILHASGIHKFKIQPKVR 78
           +F E+ W       SD+ L C     L   +F    LP IFLE      + K+KIQ K  
Sbjct: 3   SFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLE--RQGFLSKYKIQTKNN 62

Query: 79  LPFSEIFRCYKDVMRMFFLVVGPLQLVSFPSIQMIGIRTGLPLPSGWEIVSQLVVYFMVE 138
            P ++  +C   ++   F V  PL L S+P  + +G+R+  PLPS  E+ +Q++ YF++E
Sbjct: 63  TPAAQ-GKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIE 122

Query: 139 DYTNYWIHRFLHCKWGYENIHQVHHEYTAPIGFAAPYAHWAEVLILGIPSFLGPAMVPGH 198
           D+  YW HR LH KW Y+N+H VHHEY  P G  + YAH AE+L LG  + +GPA+   H
Sbjct: 123 DFVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPH 182

Query: 199 MITFWLWIALRQIEAIDTHSGYDFPWSLTKFIPFYGGADHHDYHHYVGGQSHSNFASVFT 258
           +IT WLW+ LR +E ++ H GY FPWSL+ F+P YGGAD HDYHH +      N++S F 
Sbjct: 183 LITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFV 242

Query: 259 YCDYIYGTDKGYRYQKKILKKLKE 282
           Y D+I+GTDKGYR     LK LKE
Sbjct: 243 YMDWIFGTDKGYRR----LKTLKE 259

The following BLAST results are available for this feature:

BLAST of CmaCh16G010380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1EC69	6.5e-133	75.35	Methylsterol monooxygenase 1-2 OS=Arabidopsis thaliana OX=3702 GN=SMO1-2 PE=2 SV...	[more]
Q8L7W5	1.5e-129	73.61	Methylsterol monooxygenase 1-1 OS=Arabidopsis thaliana OX=3702 GN=SMO1-1 PE=1 SV...	[more]
F4JLZ6	1.1e-119	68.07	Methylsterol monooxygenase 1-3 OS=Arabidopsis thaliana OX=3702 GN=SMO1-3 PE=2 SV...	[more]
Q9AV39	3.9e-117	69.29	Very-long-chain aldehyde decarbonylase GL1-10 OS=Oryza sativa subsp. japonica OX...	[more]
Q8VWZ8	2.6e-57	44.32	Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV...	[more]

BLAST of CmaCh16G010380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22756.1	4.7e-134	75.35	sterol C4-methyl oxidase 1-2	[more]
AT4G12110.1	1.1e-130	73.61	sterol-4alpha-methyl oxidase 1-1	[more]
AT4G22753.1	7.7e-121	68.07	sterol 4-alpha methyl oxidase 1-3	[more]
AT4G22753.2	7.7e-121	68.07	sterol 4-alpha methyl oxidase 1-3	[more]
AT1G07420.1	1.9e-58	44.32	sterol 4-alpha-methyl-oxidase 2-1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 130..265 e-value: 7.6E-30 score: 104.0
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 1..287
None	No IPR available	PANTHER	PTHR11863:SF197	METHYLSTEROL MONOOXYGENASE 1-2	coord: 1..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G010380.1	CmaCh16G010380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000254	C-4 methylsterol oxidase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity