CmaCh16G010300 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G010300 vs. ExPASy Swiss-Prot
Match: Q6YI46 (Transmembrane protein 64 OS=Homo sapiens OX=9606 GN=TMEM64 PE=2 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 1.2e-04
Identity = 49/200 (24.50%), Postives = 91/200 (45.50%), Query Frame = 0

Query: 65  WFRLVVLFIFLVSLAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVLVFASVALFPS 124
           W R +VL   L +L       V  +  +     ++ W     S  +L VL+F  V  F  
Sbjct: 119 WCRSLVLVCVLAALCFASLALVRRYLHH-----LLLWVESLDS--LLGVLLF--VVGFIV 178

Query: 125 LFLPSSPSMWLAGMTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRK-IQGW----LDKYP 184
           +  P      +  +  GY +GF+L +  + +GV +  FI  +  ++ +  W    +    
Sbjct: 179 VSFPCGWGYIVLNVAAGYLYGFVLGMGLMMVGVLIGTFIAHVVCKRLLTAWVAARIQSSE 238

Query: 185 KRASVLRLAGEGDGIHQFRAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIGMVPEI 244
           K ++V+R+   G G+   + VAL R++P P+ + N     T++    Y++ S +G++P  
Sbjct: 239 KLSAVIRVVEGGSGL---KVVALARLTPIPFGLQNAVFSITDLSLPNYLMASSVGLLPTQ 298

Query: 245 FVTIYTGILIRTLADASQNQ 260
            +  Y G  +RT+ D    Q
Sbjct: 299 LLNSYLGTTLRTMEDVIAEQ 306

BLAST of CmaCh16G010300 vs. ExPASy Swiss-Prot
Match: Q8L586 (Uncharacterized membrane protein At4g09580 OS=Arabidopsis thaliana OX=3702 GN=At4g09580 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.0e-04
Identity = 38/152 (25.00%), Postives = 69/152 (45.39%), Query Frame = 0

Query: 113 VLVFASVALFPSLF-LPSSPSM-WLAGMTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRK 172
           +L + S  +F   F +P +  M  LAG  FG   GF+L++   T G    FF+  L  R 
Sbjct: 112 ILGYCSTYIFMQTFMIPGTIFMSLLAGALFGVVRGFVLVVLNATAGACSCFFLSKLVGRP 171

Query: 173 IQGWLDKYPKRASVLRLAGEGDGIHQFRAVALIRISP-FPYIVYNYCAVATNVRYGPYIL 232
           +  WL     R     +A   D +  +  +  +RI+P  P +  N  +   ++ +  + L
Sbjct: 172 LVNWLWPEKLRFFQAEIAKRRDRLLNY--MLFLRITPTLPNLFINLSSPIVDIPFHVFFL 231

Query: 233 GSLIGMVPEIFVTIYTGILIRTLADASQNQQF 262
            +L+G++P  ++T+  G+ +  L        F
Sbjct: 232 ATLVGLMPASYITVRAGLALGDLRSVKDLYDF 261

BLAST of CmaCh16G010300 vs. TAIR 10
Match: AT1G12450.1 (SNARE associated Golgi protein family )

HSP 1 Score: 338.2 bits (866), Expect = 6.9e-93
Identity = 176/304 (57.89%), Postives = 222/304 (73.03%), Query Frame = 0

Query: 1   MTYCETDNGRRGEVVAEDITVGIGCDDGNGDYVRLGQNPCNCEAEVSSDGGASQPCSPGR 60
           MTY    NG  G V   ++ + +  D  NGDY++L +   N E E SS   +  P     
Sbjct: 1   MTY---SNGVDGTV--PELKLRVEEDSDNGDYLKL-RGGSNEEDEGSSAESSRCPIGSVT 60

Query: 61  SLWLWFRLVVLFIFLVSLAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVLVFASVA 120
           S+W W +L+ L + L SLA V  KWVGPF + KE+IP INW   TFS PVL +L+FASVA
Sbjct: 61  SVWFWVKLISLVVCLGSLAFVIIKWVGPFLIEKELIPFINWVRNTFSIPVLGLLLFASVA 120

Query: 121 LFPSLFLPSSPSMWLAGMTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRKIQGWLDKYPK 180
           LFPS+ LPSSPSMW+AG+TFGYG GFLLI+SA +IGV+LPF IG LF  K+Q WL KYPK
Sbjct: 121 LFPSILLPSSPSMWMAGLTFGYGKGFLLILSAASIGVTLPFLIGHLFLHKMQEWLKKYPK 180

Query: 181 RASVLRLAGEGDGIHQFRAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIGMVPEIF 240
           +A++LR AGEG   HQF+AV LIR+SPFPYI+YNYCA+AT V YGPYILGSL+GMVPEIF
Sbjct: 181 KAAILRAAGEGTWFHQFQAVTLIRVSPFPYIIYNYCALATGVHYGPYILGSLVGMVPEIF 240

Query: 241 VTIYTGILIRTLADASQNQQFLSAPQIAFTIVGFCVTAATTVFFTVYAKRKLNELQTEDE 300
           V+IYTGI++RTLA AS  +  LS  +I   ++GFCVTA+ T+  T+YAK+KL+ +Q+ED 
Sbjct: 241 VSIYTGIMLRTLAVASDTRHTLSVVEIVVNVLGFCVTASATIVCTIYAKKKLSAMQSEDV 298

Query: 301 QLLQ 305
           + LQ
Sbjct: 301 ETLQ 298

BLAST of CmaCh16G010300 vs. TAIR 10
Match: AT4G22850.1 (SNARE associated Golgi protein family )

HSP 1 Score: 326.6 bits (836), Expect = 2.1e-89
Identity = 165/288 (57.29%), Postives = 211/288 (73.26%), Query Frame = 0

Query: 18  DITVGIGCDDGNGDYVRLGQNPCNCEAEVSSDGGASQPCSPGRSLWLWFRLVVLFIFLVS 77
           ++ V +  DD  G YV+L +     E    S+    +  +     W W +L +LF FL +
Sbjct: 12  ELLVRVEDDDEKGPYVKLSE---VLEKGQESEQEKEEEKTDSSRFWFWVKLSLLFAFLAA 71

Query: 78  LAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVLVFASVALFPSLFLPSSPSMWLAG 137
           LAVV + W+GP  M+KE+IP+I WE  TF+ PV  +LVFASVA+FP++ LPS+PSMW+AG
Sbjct: 72  LAVVGYIWIGPLIMDKELIPLIQWEIRTFTHPVCGLLVFASVAIFPTILLPSTPSMWIAG 131

Query: 138 MTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRKIQGWLDKYPKRASVLRLAGEGDGIHQF 197
           MTFGYG+GFLLIISA  +GVSLP+FIG LF  KIQGWL++YP +A+VLR AGEG+ +HQF
Sbjct: 132 MTFGYGYGFLLIISAAAVGVSLPYFIGQLFCHKIQGWLERYPDQAAVLRAAGEGNWLHQF 191

Query: 198 RAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIGMVPEIFVTIYTGILIRTLADASQ 257
             V LIRISPFPYI+YNYC+VAT V+YGPYI GSL+GMVPE+FV IYTGIL+RTLA+AS 
Sbjct: 192 LLVTLIRISPFPYILYNYCSVATRVKYGPYITGSLLGMVPEVFVAIYTGILVRTLAEASS 251

Query: 258 -NQQFLSAPQIAFTIVGFCVTAATTVFFTVYAKRKLNELQTEDEQLLQ 305
             +Q LS  Q+   I+GF  T ATT+  T YAKR+L  ++ EDE LLQ
Sbjct: 252 AEEQGLSVTQVILNILGFLATVATTILITKYAKRQLETMKKEDEALLQ 296

BLAST of CmaCh16G010300 vs. TAIR 10
Match: AT4G12000.1 (SNARE associated Golgi protein family )

HSP 1 Score: 303.5 bits (776), Expect = 1.9e-82
Identity = 165/310 (53.23%), Postives = 208/310 (67.10%), Query Frame = 0

Query: 1   MTYCETDNGRRGEVVAEDITVGIGCD--DGNGDYVRLGQ----NPCNCEAEVSSDGGASQ 60
           MTY E D+         +  V +  D  D  G YV+L +    +    E E SS   +S 
Sbjct: 1   MTYHEEDD------TVSEFRVRVEEDGVDKLGHYVKLTEDFEVHRQETEQESSSSPSSSS 60

Query: 61  PCSPGRSLWLWFRLVVLFIFLVSLAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVL 120
            C   RS+W W +L + F FL +L +  +KW+ P  M+KE+IP+I WE ETF+ PV  +L
Sbjct: 61  SCGQKRSVWFWIKLGLFFTFLTALGLAGYKWLYPLIMDKELIPLIKWEMETFTHPVCGIL 120

Query: 121 VFASVALFPSLFLPSSPSMWLAGMTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRKIQGW 180
           VFASV+LFP + +P++PSMW+AG+TFGY +G LL + AV IGVSLP+FI  LF  KIQGW
Sbjct: 121 VFASVSLFPVILIPTTPSMWVAGITFGYFYGLLLTLPAVAIGVSLPYFISYLFLNKIQGW 180

Query: 181 LDKYPKRASVLRLAGEGDGIHQFRAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIG 240
           L++YP +A++LR AG G   HQFRAV LIRISPFP+ VYNYCAVAT V++GPY+ GSL+G
Sbjct: 181 LERYPDQAAMLRAAGGGSWFHQFRAVTLIRISPFPFAVYNYCAVATRVKFGPYMAGSLVG 240

Query: 241 MVPEIFVTIYTGILIRTLADAS-QNQQFLSAPQIAFTIVGFCVTAATTVFFTVYAKRKLN 300
           M PEIFV IYTGILIRTLADAS   Q+ LS  QI   I GF  T  TTV  T YAKR+L 
Sbjct: 241 MAPEIFVAIYTGILIRTLADASTAEQKGLSILQIVLNIFGFVATVVTTVLITKYAKRQLE 300

Query: 301 ELQTEDEQLL 304
            ++ E E LL
Sbjct: 301 VMKKEKEALL 304

BLAST of CmaCh16G010300 vs. TAIR 10
Match: AT4G22850.2 (SNARE associated Golgi protein family )

HSP 1 Score: 291.2 bits (744), Expect = 9.7e-79
Identity = 142/243 (58.44%), Postives = 183/243 (75.31%), Query Frame = 0

Query: 18  DITVGIGCDDGNGDYVRLGQNPCNCEAEVSSDGGASQPCSPGRSLWLWFRLVVLFIFLVS 77
           ++ V +  DD  G YV+L +     E    S+    +  +     W W +L +LF FL +
Sbjct: 12  ELLVRVEDDDEKGPYVKLSE---VLEKGQESEQEKEEEKTDSSRFWFWVKLSLLFAFLAA 71

Query: 78  LAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVLVFASVALFPSLFLPSSPSMWLAG 137
           LAVV + W+GP  M+KE+IP+I WE  TF+ PV  +LVFASVA+FP++ LPS+PSMW+AG
Sbjct: 72  LAVVGYIWIGPLIMDKELIPLIQWEIRTFTHPVCGLLVFASVAIFPTILLPSTPSMWIAG 131

Query: 138 MTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRKIQGWLDKYPKRASVLRLAGEGDGIHQF 197
           MTFGYG+GFLLIISA  +GVSLP+FIG LF  KIQGWL++YP +A+VLR AGEG+ +HQF
Sbjct: 132 MTFGYGYGFLLIISAAAVGVSLPYFIGQLFCHKIQGWLERYPDQAAVLRAAGEGNWLHQF 191

Query: 198 RAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIGMVPEIFVTIYTGILIRTLADASQ 257
             V LIRISPFPYI+YNYC+VAT V+YGPYI GSL+GMVPE+FV IYTGIL+RTLA+AS 
Sbjct: 192 LLVTLIRISPFPYILYNYCSVATRVKYGPYITGSLLGMVPEVFVAIYTGILVRTLAEASS 251

Query: 258 NQQ 261
            ++
Sbjct: 252 AEE 251

BLAST of CmaCh16G010300 vs. TAIR 10
Match: AT2G02370.1 (SNARE associated Golgi protein family )

HSP 1 Score: 236.5 bits (602), Expect = 2.8e-62
Identity = 116/245 (47.35%), Postives = 162/245 (66.12%), Query Frame = 0

Query: 62  LWLWFRLVVLFIFLVSLAVVFFKWVGPFFMNKEIIPIINWEAETFSTPVLAVLVFASVAL 121
           +W W + + +    + L +VF KW  PF   K +IPI+ WEA  F  P+LA+++  S+AL
Sbjct: 53  MW-WLKALGICAVALLLTLVFGKWGVPFVFQKVLIPILQWEATAFGRPMLAIVLVVSLAL 112

Query: 122 FPSLFLPSSPSMWLAGMTFGYGFGFLLIISAVTIGVSLPFFIGSLFYRKIQGWLDKYPKR 181
           FP   +PS PSMWLAGM FGYG GF++I+   TIG+ LP+ IG +F  ++  WL ++P++
Sbjct: 113 FPVFLIPSGPSMWLAGMIFGYGLGFVIIMVGTTIGMVLPYLIGLMFRDRLHQWLKRWPRQ 172

Query: 182 ASVLRLAGEGDGIHQFRAVALIRISPFPYIVYNYCAVATNVRYGPYILGSLIGMVPEIFV 241
           A+VLRLA EG   HQFR VA+ R+SPFPY ++NY  V T++R+ PY  GS+ GM+PE F+
Sbjct: 173 AAVLRLAAEGSWFHQFRVVAIFRVSPFPYTIFNYAIVVTSMRFWPYFFGSIAGMIPEAFI 232

Query: 242 TIYTGILIRTLADASQNQQFLSAPQIAFTIVGFCVTAATTVFFTVYAKRKLNELQ----T 301
            IY+G LIRT AD     Q L+  +I + ++   +   TTV FTVYAKR L ELQ     
Sbjct: 233 YIYSGRLIRTFADVQYGHQRLTTVEIVYNVISLVIAVVTTVAFTVYAKRALRELQNAEAN 292

Query: 302 EDEQL 303
           EDE++
Sbjct: 293 EDEEV 296