CmaCh16G010220 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G010220 vs. ExPASy Swiss-Prot
Match: Q7XU38 (Cytochrome P450 87A3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP87A3 PE=2 SV=3)

HSP 1 Score: 143.3 bits (360), Expect = 3.1e-33
Identity = 85/212 (40.09%), Postives = 120/212 (56.60%), Query Frame = 0

Query: 24  GRKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFE 83
           GRK  M++L+ M++ER A P +   DFFD+V++EL +   LLT+ +ALDLMF LLFASFE
Sbjct: 266 GRKNAMKVLRGMMKERMAEPERPCEDFFDHVIQELRREKPLLTETIALDLMFVLLFASFE 325

Query: 84  TTSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATR 143
           TT+LALT+ +K L ++P+V      EHE I++ R   +SG+TWAEYKSMTFT Q      
Sbjct: 326 TTALALTIGVKLLTENPKVVDALREEHEAIIRNRKDPNSGVTWAEYKSMTFTSQVIMEIV 385

Query: 144 KCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGW---IVVPSLSAKSD------------ 203
           + +++      +    + IK       + IPAGW   +  P++    +            
Sbjct: 386 RLANIVPGIFRKALQDVEIK------GYTIPAGWGIMVCPPAVHLNPEIYEDPLAFNPWR 445

Query: 204 -------GGASKHLMAFGGGLRFCFGAKFIKL 208
                   G +KH MAFGGGLRFC G    K+
Sbjct: 446 WQGKPEITGGTKHFMAFGGGLRFCVGTDLSKV 471

BLAST of CmaCh16G010220 vs. ExPASy Swiss-Prot
Match: K7NBR2 (Cucurbitadienol 11-hydroxylase OS=Siraitia grosvenorii OX=190515 GN=CYP87D18 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.8e-17
Identity = 67/209 (32.06%), Postives = 99/209 (47.37%), Query Frame = 0

Query: 26  KRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFETT 85
           K   + L+ ++ +R AN      DF    L++ E     +++E  + L+F + FASFE+ 
Sbjct: 229 KEIQKKLREVVDDRLANVGPDVEDFLGQALKDKESE-KFISEEFIIQLLFSISFASFESI 288

Query: 86  SLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATRKC 145
           S  LTL +K L +HP V      EHE I K R   D  +TW EYKSMTFT Q    T + 
Sbjct: 289 STTLTLILKLLDEHPEVVKELEAEHEAIRKARADPDGPITWEEYKSMTFTLQVINETLRL 348

Query: 146 SSVDISRSIEGCLSIRIKRGLYGARFVIPAGW-IVVPSLSAKSDGGA------------- 205
            SV  +   +    +++K       ++IP GW I++ + S   D                
Sbjct: 349 GSVTPALLRKTVKDLQVK------GYIIPEGWTIMLVTASRHRDPKVYKDPHIFNPWRWK 408

Query: 206 -------SKHLMAFGGGLRFCFGAKFIKL 208
                   K+ M FGGGLR C GA++ K+
Sbjct: 409 DLDSITIQKNFMPFGGGLRHCAGAEYSKV 430

BLAST of CmaCh16G010220 vs. ExPASy Swiss-Prot
Match: Q8L7D5 (Cytochrome P450 708A2 OS=Arabidopsis thaliana OX=3702 GN=CYP708A2 PE=2 SV=3)

HSP 1 Score: 85.1 bits (209), Expect = 1.0e-15
Identity = 65/211 (30.81%), Postives = 106/211 (50.24%), Query Frame = 0

Query: 25  RKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFET 84
           R+  ++++K++   RKA+ R+   DF D ++EE EK   +  +E A++L+F +L  + E+
Sbjct: 231 RRYALQVIKDVFTRRKAS-REMCGDFLDTMVEEGEKEDVIFNEESAINLIFAILVVAKES 290

Query: 85  TSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYK-SMTFTFQFNAATR 144
           TS   +LAIKFL ++ +       EH  IL+ R+   +G++W EY+  MTFT      T 
Sbjct: 291 TSSVTSLAIKFLAENHKALAELKREHAAILQNRNGKGAGVSWEEYRHQMTFTNMVINETL 350

Query: 145 KCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVV---PSL----------------- 204
           + +++      +    + IK       + IPAGWIV    P++                 
Sbjct: 351 RMANMAPIMYRKAVNDVEIK------GYTIPAGWIVAVIPPAVHFNDAIYENPLEFNPWR 410

Query: 205 -SAKSDGGASKHLMAFGGGLRFCFGAKFIKL 208
              K     SK  M FGGG+R C GA+F +L
Sbjct: 411 WEGKELRSGSKTFMVFGGGVRQCVGAEFARL 434

BLAST of CmaCh16G010220 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 8.8e-12
Identity = 64/210 (30.48%), Postives = 93/210 (44.29%), Query Frame = 0

Query: 25  RKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFET 84
           R+  +R + NM++ER+ +P   H D    +LEE       ++ E+  D + FLLFA  ET
Sbjct: 241 RELLIRKISNMIKERRDHPHIVHKDLLTKLLEE-----DSISDEIICDFILFLLFAGHET 300

Query: 85  TSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATRK 144
           +S A+T AIKFL   P+       EH+ ILK +      L W +YKSM FT      T +
Sbjct: 301 SSRAMTFAIKFLTTCPKALTQMKEEHDAILKAKG-GHKKLEWDDYKSMKFTQCVINETLR 360

Query: 145 CSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVVPSLSAK------------------ 204
             +       E     ++K  L      IP GW+V   L+A                   
Sbjct: 361 LGNFGPGVFRETKEDTKVKDCL------IPKGWVVFAFLTATHLDEKFHNEALTFNPWRW 420

Query: 205 --SDGGASKHLMA-FGGGLRFCFGAKFIKL 208
                 ++ HL + FGGG R C G+   +L
Sbjct: 421 ELDQDVSNNHLFSPFGGGARLCPGSHLARL 438

BLAST of CmaCh16G010220 vs. ExPASy Swiss-Prot
Match: Q8GSQ1 (Cytochrome P450 85A1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP85A1 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-11
Identity = 59/203 (29.06%), Postives = 96/203 (47.29%), Query Frame = 0

Query: 25  RKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFET 84
           RK+ + ML+ M+ ER+++  + H+D  D +L  +E     LT E  +DL+  L+++ +ET
Sbjct: 227 RKKLVAMLEQMIAERRSS-GQVHDDMLDALLTGVEGTREKLTDEQIIDLIITLIYSGYET 286

Query: 85  TSLALTLAIKFLVDHP------RVEHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATRK 144
            S    +A+K+L DHP      R EH  I K +   D+ + W ++KSMTFT      T +
Sbjct: 287 MSTTSMMAVKYLSDHPKALEQLRKEHFDIRKGKAPEDA-IDWNDFKSMTFTRAVIFETLR 346

Query: 145 CSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVV--------------------PSLS 202
            ++V     + G L  +  + +    +VIP GW +                     P   
Sbjct: 347 LATV-----VNGLLR-KTTQDVEMNGYVIPKGWRIYVYTREINYDPFLYPDPMTFNPWRW 406

BLAST of CmaCh16G010220 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 187.2 bits (474), Expect = 1.3e-47
Identity = 108/211 (51.18%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 24  GRKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFE 83
           GR + M+ML+NMLQER+ NPRK  +DFFDYV+EE++K GT+LT+E+ALDLMF LLFASFE
Sbjct: 224 GRAKAMKMLRNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFE 283

Query: 84  TTSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATR 143
           TTSLALTLAIKFL D P V      EHE IL+ R+ ADSGLTW EYKSMT+TFQF   T 
Sbjct: 284 TTSLALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETA 343

Query: 144 KCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVV---PSLSAKSD------------ 203
           + +++  +   +    I+ K       + IPAGW V+   P++    +            
Sbjct: 344 RLANIVPAIFRKALRDIKFK------DYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSR 403

Query: 204 ------GGASKHLMAFGGGLRFCFGAKFIKL 208
                   ASKH MAFGGG+RFC G  F KL
Sbjct: 404 WEGSKVTNASKHFMAFGGGMRFCVGTDFTKL 428

BLAST of CmaCh16G010220 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 187.2 bits (474), Expect = 1.3e-47
Identity = 108/211 (51.18%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 24  GRKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFE 83
           GR + M+ML+NMLQER+ NPRK  +DFFDYV+EE++K GT+LT+E+ALDLMF LLFASFE
Sbjct: 230 GRAKAMKMLRNMLQERRENPRKNPSDFFDYVIEEIQKEGTILTEEIALDLMFVLLFASFE 289

Query: 84  TTSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKSMTFTFQFNAATR 143
           TTSLALTLAIKFL D P V      EHE IL+ R+ ADSGLTW EYKSMT+TFQF   T 
Sbjct: 290 TTSLALTLAIKFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETA 349

Query: 144 KCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVV---PSLSAKSD------------ 203
           + +++  +   +    I+ K       + IPAGW V+   P++    +            
Sbjct: 350 RLANIVPAIFRKALRDIKFK------DYTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSR 409

Query: 204 ------GGASKHLMAFGGGLRFCFGAKFIKL 208
                   ASKH MAFGGG+RFC G  F KL
Sbjct: 410 WEGSKVTNASKHFMAFGGGMRFCVGTDFTKL 434

BLAST of CmaCh16G010220 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 105.1 bits (261), Expect = 6.7e-23
Identity = 74/214 (34.58%), Postives = 109/214 (50.93%), Query Frame = 0

Query: 25  RKRTMRMLKNMLQERK---ANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFAS 84
           R   M+MLK M +ER+    +   ++ DF + ++ E+EK G  + +E +++L+  LL AS
Sbjct: 394 RSNVMKMLKRMFKERREEATSDDSKYGDFMETMIYEVEKEGDTINEERSVELILSLLIAS 453

Query: 85  FETTSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYKS-MTFTFQFNA 144
           +ETTS    L +KF+ ++P+V      EHE IL+ R   +SG+TW EY+S M FT     
Sbjct: 454 YETTSTMTALTVKFIAENPKVLMELKREHETILQNRADKESGVTWKEYRSMMNFTHMVIN 513

Query: 145 ATRKCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWI--VVPSL--------------- 204
            + +  S+  +   +    + IK       + IPAGWI  VVPSL               
Sbjct: 514 ESLRLGSLSPAMFRKAVNDVEIK------GYTIPAGWIVLVVPSLLHYDPQIYEQPCEFN 573

Query: 205 ----SAKSDGGASKHLMAFGGGLRFCFGAKFIKL 208
                 K     SK  MAFGGG R C GA+F +L
Sbjct: 574 PWRWEGKELLSGSKTFMAFGGGARLCAGAEFARL 601

BLAST of CmaCh16G010220 vs. TAIR 10
Match: AT3G44970.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.0e-18
Identity = 69/207 (33.33%), Postives = 105/207 (50.72%), Query Frame = 0

Query: 29  MRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFETTSLA 88
           MR +K++   RK +  K ++DF +  +EE EK G LL +   + L+F L   + +TTS A
Sbjct: 235 MREIKDIYTMRKTSEEK-YDDFLNTAIEESEKAGELLNENAIITLIFTLSCVTQDTTSKA 294

Query: 89  LTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYK-SMTFTFQFNAATRKCSS 148
           + LA+KFL+++P+V      EHE IL+ R+  + G+TW EY+  MTFT      + + ++
Sbjct: 295 ICLAVKFLLENPKVLAELKKEHEVILESREDKEGGVTWEEYRHKMTFTNMVINESLRITN 354

Query: 149 VDISRSIEGCLSIRIKRGLYGARFVIPAGWIV--VPSL-------------------SAK 208
           +      +    + IK       + IPAGWIV  +PS+                     K
Sbjct: 355 LAPMLFRKAVKDVEIK------GYTIPAGWIVMIIPSVVHFDPEIYENPFEFNPWRWEGK 414

BLAST of CmaCh16G010220 vs. TAIR 10
Match: AT1G78490.1 (cytochrome P450, family 708, subfamily A, polypeptide 3 )

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-18
Identity = 69/211 (32.70%), Postives = 104/211 (49.29%), Query Frame = 0

Query: 25  RKRTMRMLKNMLQERKANPRKQHNDFFDYVLEELEKHGTLLTQEMALDLMFFLLFASFET 84
           R+  ++++K++L  RK    KQ  DF + +LEELEK G+   Q  A++L+F L FA  E 
Sbjct: 231 REEAIQVMKDVLMMRKETREKQ-EDFLNTLLEELEKDGSFFDQGSAINLIFLLAFALREG 290

Query: 85  TSLALTLAIKFLVDHPRV------EHEGILKKRDKADSGLTWAEYK-SMTFTFQFNAATR 144
           TS    LA+KF+   P+V      EH+ I+  R   ++G++W EY+ +MTFT   +    
Sbjct: 291 TSSCTALAVKFISKDPKVLAELKREHKAIVDNRKDKEAGVSWEEYRHNMTFTNMVSNEVL 350

Query: 145 KCSSVDISRSIEGCLSIRIKRGLYGARFVIPAGWIVVPSLSA------------------ 204
           + ++       +    + IK       + IPAGWIV  + SA                  
Sbjct: 351 RLANTTPLLFRKAVQDVEIK------GYTIPAGWIVAVAPSAVHFDPAIYENPFEFNPWR 410

Query: 205 ---KSDGGASKHLMAFGGGLRFCFGAKFIKL 208
              K     SK  MAFG G+R C GA+F +L
Sbjct: 411 WEGKEMIWGSKTFMAFGYGVRLCVGAEFSRL 434