CmaCh16G010070 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G010070
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionIntegrase catalytic domain-containing protein
LocationCma_Chr16: 7756537 .. 7758349 (+)
RNA-Seq ExpressionCmaCh16G010070
SyntenyCmaCh16G010070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAAACAAATAGGAGGCATTACATCGAAGGGTGAAGAAGAAGTACTCTACACAAGTGAAAGCCGGAGCAATAATAGGCCGTCTACAAAACGCGGATACAATGGTGACAAAACAAGAAGTCACCAAGGAATTGTACAACTAGGGAGAGCTTATAAGAACGGTAACAATAACTCTCAAGGGAAAAGATTTGAGGGCATTTACTACAATTGCGGGAAGAAGGGCCACATGTCCAAAGATTGTTGGTCTAAGAAAAAATCTGTCGAAAGCAATGTGACATCCTCCAACATGGAGATGGAGGAGGAATGGGATGCAGAGGTACTCTATGCTATAGAAGAAGATGAGCTAGCACTCATGGTGATGATGGGAGACCATATCGATTATGAGAATGATTGGATCATTGATTTAGGATACTCAAACCACATGATTGATGATCAAAGTGGTGCAGTGGAAGAGGGGTGGCCCTTAGAGAAGAGTATTACCAAGCCTTGAGCAAATTGAAGAAATTCTTTTGACGGAAGAGCAAACTGAAGAGATTCTTCCACAGAAGACGGGGAGGAAACTGTACACATTTGCTTCAGTGCTAATGTGGCTGAAGATTCAAGTGACACTAGTCTTGGTGAGTAATTGGTGAGTAAGAAGTGACTCAACCAAGCGAACCTAGTAAGAAATAATGAGCACCTCAACCACTAAGACAATTAGAAATGATCCAAAAGCCAAGTCTAGAGTATGTCAACACAACTATTGTAGAAGATGAAGTTAATATAAGCTAGAGATATATGAGGATGTATCACAAAACTCGGTTTGGCAGAAAGCGATTGAGGAAGAAATTATAGCCGTGGAGCAAAATCAAACTTGAGAACTAGTGCCAAGATCAAGAGATGTTAAATCTATCTTTTGCAAGTGGATTTACAAAATAAACTGTACCCCGAATGGATCAATTGTGAGATACAAAACTCAGACTGTAGATCCAGGGTTCTCTCAACAATATGAACTAGACTATGATGAAACGTTTAGTCTAGTGGCAAAGATCATTATCGTACAAGTTTCTCCAGTACTTACGGTAAATAAAGATTGAAAATTATGGTAGATGGATATGAATAATGCTTTATTGCATGGAGAGTTAAACAGAGAGTTCTACATGGACTAACTGGAGAAATTCGAAAATGAAGTTGGAGTCATTGATCAATATATGTAAAATCCGAAGAAGCCTCATTTGGATGCGGCTCGACGAACCTTGAGATATGTCAAAGGTACAATCAATTACGATCGTTTATACAAAAGAAGCGAAGACTAGAAGCTAGCTGGATATAGTGATGTCGACTATGCAGGAGACCACGATACCCGAAGATCAATCACTGGGTATGTGTTCAAGCTTGGTTTGAGAACAATTTTTTGGTGTAACAAAAGACAACTAACAATATCATTGTCAACTAGAGAAGCAGAGTATAGAGCAGCAGCTGGAGCAGCTCAGGAAAATACATGGTTAAAACTTTTGATGGAAGATTGGCACCAGAAAATTGAGTATCTAATACTTCATTACAACAATCAATCTGTGATTCGCCTAGCAGAAAATTCGGTGTTTCATGCTAGAACAAAACATGTAGAGGTACACTATCATTTCATTAGAGAGAAAGTCTTGAAGGAACAAATGGAGATGCAGCAGATCAAGACAGATGATCAAGTGACAGACTTGTTTACAAAAGGGCTGAATACTGCTAAACATGAGAGTTTTCGCTGTCAGCTCAACATGGTGCAACGAATGAGGACTAGTGCTGAGGGGAGTCTTAAAATATCATCACTAACCCAATAG

mRNA sequence

ATGGCTAAACAAATAGGAGGCATTACATCGAAGGGTGAAGAAGAAGTACTCTACACAAGTGAAAGCCGGAGCAATAATAGGCCGTCTACAAAACGCGGATACAATGGTGACAAAACAAGAAGTCACCAAGGAATTGTACAACTAGGGAGAGCTTATAAGAACGGTAACAATAACTCTCAAGGGAAAAGATTTGAGGGCATTTACTACAATTGCGGGAAGAAGGGCCACATGTCCAAAGATTGTTGGTCTAAGAAAAAATCTGTCGAAAGCAATGTGACATCCTCCAACATGGAGATGGAGGAGGAATGGGATGCAGAGGTACTCTATGCTATAGAAGAAGATGAGCTAGCACTCATGGTGATGATGGGAGACCATATCGATTATGAGAATGATTGGATCATTGATTTAGGATACTCAAACCACATGATTGATGATCAAAGTGGAGACCACGATACCCGAAGATCAATCACTGGGTATGTGTTCAAGCTTGGTTTGAGAACAATTTTTTGGTGTAACAAAAGACAACTAACAATATCATTGTCAACTAGAGAAGCAGAGTATAGAGCAGCAGCTGGAGCAGCTCAGGAAAATACATGGTTAAAACTTTTGATGGAAGATTGGCACCAGAAAATTGAGTATCTAATACTTCATTACAACAATCAATCTGTGATTCGCCTAGCAGAAAATTCGGTGTTTCATGCTAGAACAAAACATGTAGAGGTACACTATCATTTCATTAGAGAGAAAGTCTTGAAGGAACAAATGGAGATGCAGCAGATCAAGACAGATGATCAAGTGACAGACTTGTTTACAAAAGGGCTGAATACTGCTAAACATGAGAGTTTTCGCTGTCAGCTCAACATGGTGCAACGAATGAGGACTAGTGCTGAGGGGAGTCTTAAAATATCATCACTAACCCAATAG

Coding sequence (CDS)

ATGGCTAAACAAATAGGAGGCATTACATCGAAGGGTGAAGAAGAAGTACTCTACACAAGTGAAAGCCGGAGCAATAATAGGCCGTCTACAAAACGCGGATACAATGGTGACAAAACAAGAAGTCACCAAGGAATTGTACAACTAGGGAGAGCTTATAAGAACGGTAACAATAACTCTCAAGGGAAAAGATTTGAGGGCATTTACTACAATTGCGGGAAGAAGGGCCACATGTCCAAAGATTGTTGGTCTAAGAAAAAATCTGTCGAAAGCAATGTGACATCCTCCAACATGGAGATGGAGGAGGAATGGGATGCAGAGGTACTCTATGCTATAGAAGAAGATGAGCTAGCACTCATGGTGATGATGGGAGACCATATCGATTATGAGAATGATTGGATCATTGATTTAGGATACTCAAACCACATGATTGATGATCAAAGTGGAGACCACGATACCCGAAGATCAATCACTGGGTATGTGTTCAAGCTTGGTTTGAGAACAATTTTTTGGTGTAACAAAAGACAACTAACAATATCATTGTCAACTAGAGAAGCAGAGTATAGAGCAGCAGCTGGAGCAGCTCAGGAAAATACATGGTTAAAACTTTTGATGGAAGATTGGCACCAGAAAATTGAGTATCTAATACTTCATTACAACAATCAATCTGTGATTCGCCTAGCAGAAAATTCGGTGTTTCATGCTAGAACAAAACATGTAGAGGTACACTATCATTTCATTAGAGAGAAAGTCTTGAAGGAACAAATGGAGATGCAGCAGATCAAGACAGATGATCAAGTGACAGACTTGTTTACAAAAGGGCTGAATACTGCTAAACATGAGAGTTTTCGCTGTCAGCTCAACATGGTGCAACGAATGAGGACTAGTGCTGAGGGGAGTCTTAAAATATCATCACTAACCCAATAG

Protein sequence

MAKQIGGITSKGEEEVLYTSESRSNNRPSTKRGYNGDKTRSHQGIVQLGRAYKNGNNNSQGKRFEGIYYNCGKKGHMSKDCWSKKKSVESNVTSSNMEMEEEWDAEVLYAIEEDELALMVMMGDHIDYENDWIIDLGYSNHMIDDQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTWLKLLMEDWHQKIEYLILHYNNQSVIRLAENSVFHARTKHVEVHYHFIREKVLKEQMEMQQIKTDDQVTDLFTKGLNTAKHESFRCQLNMVQRMRTSAEGSLKISSLTQ
Homology
BLAST of CmaCh16G010070 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 8.2e-22
Identity = 59/138 (42.75%), Postives = 89/138 (64.49%), Query Frame = 0

Query: 145  DQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTWLKLLM 204
            D +GD D R+S TGY+F      I W +K Q  ++LST EAEY AA    +E  WLK  +
Sbjct: 1182 DMAGDIDNRKSSTGYLFTFSGGAISWQSKLQKCVALSTTEAEYIAATETGKEMIWLKRFL 1241

Query: 205  ED--WHQKIEYLILHYNNQSVIRLAENSVFHARTKHVEVHYHFIREKVLKEQMEMQQIKT 264
            ++   HQK EY +++ ++QS I L++NS++HARTKH++V YH+IRE V  E +++ +I T
Sbjct: 1242 QELGLHQK-EY-VVYCDSQSAIDLSKNSMYHARTKHIDVRYHWIREMVDDESLKVLKIST 1301

Query: 265  DDQVTDLFTKGLNTAKHE 281
            ++   D+ TK +   K E
Sbjct: 1302 NENPADMLTKVVPRNKFE 1317

BLAST of CmaCh16G010070 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 102.4 bits (254), Expect = 9.0e-21
Identity = 61/169 (36.09%), Postives = 98/169 (57.99%), Query Frame = 0

Query: 135  DLGYSNHMI----DDQSGDHDTRRSITGYVFKL-GLRTIFWCNKRQLTISLSTREAEYRA 194
            +L + N +I     D +G    R+S TGY+FK+     I W  KRQ +++ S+ EAEY A
Sbjct: 1241 NLAFENKIIGYVDSDWAGSEIDRKSTTGYLFKMFDFNLICWNTKRQNSVAASSTEAEYMA 1300

Query: 195  AAGAAQENTWLKLLMEDWHQKIEYLI-LHYNNQSVIRLAENSVFHARTKHVEVHYHFIRE 254
               A +E  WLK L+   + K+E  I ++ +NQ  I +A N   H R KH+++ YHF RE
Sbjct: 1301 LFEAVREALWLKFLLTSINIKLENPIKIYEDNQGCISIANNPSCHKRAKHIDIKYHFARE 1360

Query: 255  KVLKEQMEMQQIKTDDQVTDLFTKGLNTAKHESFRCQLNMVQRMRTSAE 298
            +V    + ++ I T++Q+ D+FTK L  A+    R +L ++Q  +++AE
Sbjct: 1361 QVQNNVICLEYIPTENQLADIFTKPLPAARFVELRDKLGLLQDDQSNAE 1409

BLAST of CmaCh16G010070 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.8e-16
Identity = 53/159 (33.33%), Postives = 84/159 (52.83%), Query Frame = 0

Query: 145  DQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTWL-KLL 204
            D +GD D   S  GY+  LG   I W +K+Q  +  S+ EAEYR+ A  + E  W+  LL
Sbjct: 1299 DWAGDTDDYVSTNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSELQWICSLL 1358

Query: 205  MEDWHQKIEYLILHYNNQSVIRLAENSVFHARTKHVEVHYHFIREKVLKEQMEMQQIKTD 264
             E   Q     +++ +N     L  N VFH+R KH+ + YHFIR +V    + +  + T 
Sbjct: 1359 TELGIQLSHPPVIYCDNVGATYLCANPVFHSRMKHIALDYHFIRNQVQSGALRVVHVSTH 1418

Query: 265  DQVTDLFTKGLNTAKHESFRCQLNMVQRMRTSAEGSLKI 303
            DQ+ D  TK L+    ++F  ++ ++ ++  S  G L+I
Sbjct: 1419 DQLADTLTKPLSRVAFQNFSRKIGVI-KVPPSCGGVLRI 1456

BLAST of CmaCh16G010070 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 2.3e-16
Identity = 48/147 (32.65%), Postives = 77/147 (52.38%), Query Frame = 0

Query: 145  DQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTWL-KLL 204
            D +GD D   S  GY+  LG   I W +K+Q  +  S+ EAEYR+ A  + E  W+  LL
Sbjct: 1316 DWAGDKDDYVSTNGYIVYLGHHPISWSSKKQKGVVRSSTEAEYRSVANTSSEMQWICSLL 1375

Query: 205  MEDWHQKIEYLILHYNNQSVIRLAENSVFHARTKHVEVHYHFIREKVLKEQMEMQQIKTD 264
             E   +     +++ +N     L  N VFH+R KH+ + YHFIR +V    + +  + T 
Sbjct: 1376 TELGIRLTRPPVIYCDNVGATYLCANPVFHSRMKHIAIDYHFIRNQVQSGALRVVHVSTH 1435

Query: 265  DQVTDLFTKGLNTAKHESFRCQLNMVQ 291
            DQ+ D  TK L+    ++F  ++ + +
Sbjct: 1436 DQLADTLTKPLSRTAFQNFASKIGVTR 1462

BLAST of CmaCh16G010070 vs. ExPASy Swiss-Prot
Match: P0CV72 (Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 2.6e-07
Identity = 29/65 (44.62%), Postives = 43/65 (66.15%), Query Frame = 0

Query: 136 LGYSNHMIDDQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQ 195
           +GYS+    D +GD ++RRS +GY+FKL    + W +K+Q T++LS+ E EY A + A Q
Sbjct: 72  VGYSD---ADWAGDVESRRSTSGYLFKLNGGCVSWRSKKQRTVALSSTEDEYMALSEATQ 131

Query: 196 ENTWL 201
           E  WL
Sbjct: 132 EAVWL 133

BLAST of CmaCh16G010070 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-13
Identity = 46/126 (36.51%), Postives = 68/126 (53.97%), Query Frame = 0

Query: 151 DTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTWLKLLMEDWHQK 210
           DTRRS  GY   LG   I W +K+Q  +S S+ EAEYRA + A  E  WL     +    
Sbjct: 455 DTRRSTNGYCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSFATDEMMWLAQFFRELQLP 514

Query: 211 I-EYLILHYNNQSVIRLAENSVFHARTKHVEVHYHFIREKVLKEQMEMQQIKTDDQVTDL 270
           + +  +L  +N + I +A N+VFH RTKH+E   H +RE+ + +       +  D+  D 
Sbjct: 515 LSKPTLLFCDNTAAIHIATNAVFHERTKHIESDCHSVRERSVYQATLSYSFQAYDE-QDG 574

Query: 271 FTKGLN 276
           FT+ L+
Sbjct: 575 FTEYLS 579

BLAST of CmaCh16G010070 vs. TAIR 10
Match: ATMG00810.1 (DNA/RNA polymerases superfamily protein )

HSP 1 Score: 52.4 bits (124), Expect = 7.6e-07
Identity = 28/55 (50.91%), Postives = 32/55 (58.18%), Query Frame = 0

Query: 145 DQSGDHDTRRSITGYVFKLGLRTIFWCNKRQLTISLSTREAEYRAAAGAAQENTW 200
           D +G   TRRS TG+   LG   I W  KRQ T+S S+ E EYRA A  A E TW
Sbjct: 171 DWAGCTSTRRSTTGFCTFLGCNIISWSAKRQPTVSRSSTETEYRALALTAAELTW 225

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P109788.2e-2242.75Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041469.0e-2136.09Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Q9ZT941.8e-1633.33Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Q94HW22.3e-1632.65Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
P0CV722.6e-0744.62Secreted RxLR effector protein 161 OS=Plasmopara viticola OX=143451 GN=RXLR161 P... [more]
Match NameE-valueIdentityDescription
AT4G23160.11.1e-1336.51cysteine-rich RLK (RECEPTOR-like protein kinase) 8 [more]
ATMG00810.17.6e-0750.91DNA/RNA polymerases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D4.10.60.10coord: 45..94
e-value: 5.7E-6
score: 28.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..40
NoneNo IPR availablePANTHERPTHR11439:SF343SUBFAMILY NOT NAMEDcoord: 162..289
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 162..289
NoneNo IPR availableCDDcd09272RNase_HI_RT_Ty1coord: 145..276
e-value: 4.06452E-50
score: 161.097
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 69..81
e-value: 1.7E-4
score: 21.5
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 69..81
score: 9.075821
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 49..89

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G010070.1CmaCh16G010070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding