CmaCh16G009990 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G009990
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsubtilisin-like protease SBT5.4
LocationCma_Chr16: 7698565 .. 7703232 (+)
RNA-Seq ExpressionCmaCh16G009990
SyntenyCmaCh16G009990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGGTTAATCATCACTTATAAATCCTTTTTGATGTTTATATCGAGTGGGTTTTAGACATTGAAACGTTTTTTTTTTTTAATTTTGTACAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGTGAAACTTTTTGTTTCAAATCATGTATGGTAGATTTGTTGAACTCTCACTCTTCATTTACGCTCTTATATTTTTGTTTCTAAGTACAGCTATGAAGCAGCTAAGGAAGCAATCTTTTACTCATACAATAGACATATCAATGCCTTTGCAGCTGTTCTTGATCAAAAGTTGTAGAAGACATAGCAAGTAAAATGATTCATCCGTTTCTATGTCTATAGCTATCATTTGGTTTGATAATGAAACTTCCTAATAAAATCAAAAACAAATGATTCATCGTTTGTTCCTAATTTATTTAGTTTCATAGTTTTATGGAATTTCAAAAACAAAAACAACCCTTTTTAGATTCCATAACTTTGTAATGGGGAAGTATTCACAGACTTAATTTCAAAAGTCAAATAGTTATCAAATAGTTATCAAGCGAGCCTCATTTTTTAATTTAATAATGATGATGTTTTTATCATTTTCTGTGTTAGAACATCCTGATGTGATATCAGTGTATGAAAACAAAGAACTAAAACTGCACACGACACGATCATGGAACTTTCTTGGAGTTGAGAATAATGATATAATTCCTTCAAACTCGCTTTGGAAACTTTCAAGATTTGGTGAATCTACAATCATTGGCAACCTTGACTCGGGTTGGTCTTACTTCCTTTTTTAAGTTCTTTTTTTTTTTTTTAGTTTTTGTAATAATTTTTATATGTAATTGGAATCAATTTAGGTGTTTGGCCAGAATCAAAGAGTTTTAGTGATGAAGGATATGGACCTATCCCAACAAGATGGAAGGGAAGTTGTGAAGGTGGCTCCAACTTTAGTTGCAACAGGTACTTACCATCGAATCTCTTATTATTACTTCACCTCTATTTAATTCTTTTATGCTAACAAATATGCACATTTTCCATATTAGACACTTTTGTTAGCCTAATAGACAAATATTGAACAATAGTCGGTGTATAATTCGCGAATATTAATGTAAAATTTATTAGAGGCATATTGAATCATTGTTAATGATACTAAATAGACATGTACATGCATAAATTTTCATTTTCTTAGTTAAATTACAAGTATAGCTCCTAAACTTTCACGTTTATGTTTAAGATATCTTTGAATTTCAAAAATTATCTAATAAGGATTTAAACTTTTATTTTTTATGTGTCTAAAATATTTTTCAAATGTGTAATAGATTTGAACATTCAAGTATGTGTCTAATATGTGAGACATATTCAGATATTTAAAAACTAATTGGTCAAATTTTCTATCTATTAAAACTCTTAACCTTCCATTTATTAAAATTACAAGATGTAAAGTCTTTTTAAAGTTTTGGAATAAAATATAGATAGACCTATCATACATTTCTTTTAGTTCAAATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTTGTAGTTTAATTTATGTTTTTAAGCATACAAAGACATAAATTCATCGACTTTTGAATTTTCTTTGTATATAAAAATAAGAGTAGACGTAATGCTTGTATCAGTACGTGTGACAATTTTAAATGCAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGTACTCTACTCAACACTTGTTAGTGCTATATGGTTGTGTTCTTGACAATATTTATATAAATATTTAGGTTAGTATCGAACAATTTTTAAACATAGTAAATTGTATTGGGCATATGAAGTTTTATCATATACATGTCTCTATATTTGTATCGATTGAGTTTATGGCTCCGTACTACGTTTATATTCTCACCAATGATTTTTCATTGTCAATATCAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCGTGAGTATATTTTTATTTAAATTATACAAAATATCTCTAAATTTTAGAAACATTTCAATCCTGCCCTCACCATTAACACTTCAATTTTTGAAACAGATCCAAAAAGAATTTTCTATTGAAACAATGTAAATCATTAAATGATGTTACCTATATCTTTTGAGTTGAACTAAGCAGTAAACGTTAATCATATTTTTTTTATATAGTTATAAGTTGTGTTATTATTATAATTTTTTCTAATTTTTAAGAATGTTGAACTTAATTTGATGGGTATTTTTCAAAATTTTTGCGAGAGTTAAATTTTAAAACATATTTTACATTTTAAAAGAGTATTTTATATAATTTAATCGGTAGGTTTTCACCTAATAATAAGAAGAAATTGCCAATAGCACATGATATTAAGCAAAATTTATTGTAATCTTGTAGCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAGTAATTTCTCCACCTTATTCCACAACTTCCATGTTTATTCAAAAAGTATTTTAAACTTTATGATCTAACCAACATAAATTTAGAAGTTTATTAATCTATTAAATGTACTTTTTAAGTTAAAAAATTATTAAACTCAAAATTTGTAACTTATATGTCATGTTTTGTTGTTGAAACAGAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGGTTGGTTAAGAACGATGTGTTGAGTACTTGTACCGTATAAAGTTCAATGTGTCATCCCCTAACAATCTCAAAATTTTAAGTACTGCATTTTTTTTTTTAATTTCAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGGTTAGTTATTCAAAATTCTTCAACCATTTTAAAAGTTAACTGAGTAAATAAATTAGTAATTTATGAATAGTAAGAGATGTTATTATGGTATTTGCATTATTTTGTATTAAGAAACATTTTGACTTAAATCTGGCTAACTAATGATTTTGTTTGGTAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

mRNA sequence

ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

Coding sequence (CDS)

ATGGAGGCCTTCAATCTTCCTCCTTTGCTTCTCTCGTTCTTTCTTTTTGCTCTTTTGCAAACATCCACCCTTGCAGCCAAGAAGTCTTATATTGTTTATTTGGGATCACATTCGCATGGGTTGAATCCTTCTGCTGTTGATCTCCAACTTGCAACACAAACCCACTACACTCTACTTGAATCCGTGTTAGGAAGGAAGCTGATTGGAGCACGGTATTTCAACAAAGGTTATGCATCCAACGTGGGACCTCTCAACTCGAGCTATGAAACGGCAAGGGACGATGATGGGCATGGAACACACACCTTATCCACAGCTGGAGGCAATTTCGTTCAAAGAGTAAGCATTTTTGGGAATGGTTATGGCACGGCAAAAGGAGGTTCCCCTAAAGCTCGTGTTGCTGCCTATAGAGTATGTTGGCCTGAGGTGGCGAATGGTGGGTGTTTTATGGCCGATATTCTAGCTGGCTTTGAAGCTGCCATTAGCGATGGAGTTGACGTTCTATCGATTTCACTCGGTGGAGGTGCTATAGAATTTTCTGAAGATCTACTAGCTATAATGTCCTACCATACAGTGAAGAACGACATCACTGTTGTTTGTTCGGCTGGAAATTCAGGACCATTTGCAGGTACTGTCTCAAATGTCGCACCATGGATGATAACGGTGGGAGCTAGCACAGTTGACAGGCTTTTTACAAGTTATGTGGCGTTGGGAGACAAGAGGCACTTCAAGGGTAAAAGTCTTTCTAGTAAATTATTGCCAACTAAGAAACTCTATCCGTTGATCCGTGCTTTAGATGCAAAATCCAACAATGTCTCTGATAACTACGCCATACTATGTCGCAAAGGGTCTCTTGATCCTAAAAAGGTAAAAGGAAAAATTGTAGTTTGCCTTAGAGGGGACAATGCAAGAATCGAGAAAGGTTATGTGGTTGCTCAAGCAGGTGCTGTTGGGATGATTCTTGCTAATGACAAGGAGAATGGAGATGCATTCGAGGCTGATGCGCATTTACTTCCTGCTTCACATATAAGCTATACCGACGGTGAATCAGTCTACCAATACATCCAGTCTACTAAAACTCCAATGGCTTACATGACTCACATGACTGAGTTGGGAATCAAACCCGCACCAATTATGGCTTCATTCTCATCAAGAGGTCCCAACACAATTGAGCCCTCAATACTCAAGCCTGACATAATAGCACCAGGTGTAAATATAATAGCGGCTTTCTCTGAAGCAACATCCATAACTGGTTTACCTTATGATAAGCGTATGGTTCAATTCACTGAATTATCTGGCACTTCTATGTCATGCCCCCATATTTCTGGCATTGTTGGTCTTCTCAAAACTCTTTATCCAAAATGGAGTCCAGCGGCTATCAGGTCTGCAATCATGACCACAGCTGGAACGGAAGCCAATGACTTAAATCCAATACTAAGCTCAGAAAAGGAGAAAGCAAACCCATTGGCATATGGTGCAGGCCATGTCCAACCAAATAAAGCAGCAAATCCTGGCCTTGTTTACGATCTCTCCATCCAAGACTACTTGAACTTCTTATGTGCTCGTGGCTACAATAAAGCACAAATGAAACTATTCACTAATGATACTTCATTTGTTTGTTCAAAGTCATTCAAAGTAATAGACTTGAATTATCCATCAATCTCGATATATAATCTAAAATCAGAGGTAGTGAATATCAAAAGAAGAGTGAAGAATGTAGGAAGTCCAGGCACCTATGTTGCTAAAGTCGAGTCACCGCCGGGAGTTTCAGTTTTGGTAGCACCAAGTACTTTGAAGTTCACTAAAACGGGTGAAGAGAAGAATTTCAAAGTTGTGTTGAGGAGGGTGCAAAGTAATCAAACTGAAGAGCATGTGTTCGGAAAACTTGTGTGGTCTGATGGGAAACATCGTGTTAGTAGTCCAATTTTTGTGACATTAGAGATTTAG

Protein sequence

MEAFNLPPLLLSFFLFALLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSFKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTLEI
Homology
BLAST of CmaCh16G009990 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 5.5e-193
Identity = 353/583 (60.55%), Postives = 423/583 (72.56%), Query Frame = 0

Query: 65  RKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTA 124
           RKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG G GTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 125 KGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
            GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A ++  D +
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           AI S+H VKN +TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F ++V L + + FK
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G SL SK LP +K+Y LI A DA   N +   A+LC+KGSLDPKKVKGKI+VCLRGDNAR
Sbjct: 377 GTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 436

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           ++KG   A AGA GM+L NDK +G+   +DAH+LPAS I Y DGE+++ Y+ STK P  Y
Sbjct: 437 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 496

Query: 365 MTHMT-ELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           +   T  L  KPAP MASFSSRGPNTI P ILKPDI APGVNIIAAF+EAT  T L  D 
Sbjct: 497 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 556

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R   F   SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIMTT+ T  N   P++    
Sbjct: 557 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 616

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           +KANP +YG+GHVQPNKAA+PGLVYDL+  DYL+FLCA GYN   ++LF  D  + C + 
Sbjct: 617 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 676

Query: 545 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKT 604
             ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV V V P  L F KT
Sbjct: 677 ANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 605 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           GE K F++ LR +    +  +VFG+L W+D  H V SPI V L
Sbjct: 737 GEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of CmaCh16G009990 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.5e-190
Identity = 339/582 (58.25%), Postives = 422/582 (72.51%), Query Frame = 0

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VSIFG G GTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
           GGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I S+H  K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S + LG+ +H+KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
           +SLSS  LP  K YP++ +++AK+ N S   A LC+ GSLDP K KGKI+VCLRG N R+
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           EKG  VA  G +GM+L N    G+   AD H+LPA+ ++  D  +V +YI  TK P+A++
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 365 T-HMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           T   T+LG+KPAP+MASFSS+GP+ + P ILKPDI APGV++IAA++ A S T   +D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F  +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI ++   
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KA P ++GAGHVQPN A NPGLVYDL I+DYLNFLC+ GYN +Q+ +F+ +     S   
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
            +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V V P++L FTK G
Sbjct: 671 SLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 730

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           E+K FKV+L + + N  + +VFG+LVWSD KHRV SPI V L
Sbjct: 731 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh16G009990 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 4.5e-147
Identity = 297/634 (46.85%), Postives = 402/634 (63.41%), Query Frame = 0

Query: 18  LLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGY 77
           +L T      KSY         G  PS+        T++T   S+  RKLIGAR+F +GY
Sbjct: 137 VLDTGVWPESKSY----SDEGFGPIPSSWKGGCEAGTNFT--ASLCNRKLIGARFFARGY 196

Query: 78  ASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAY 137
            S +GP++ S E  + RDDDGHGTHT STA G+ V+  S+ G   GTA+G +P+ARVA Y
Sbjct: 197 ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVY 256

Query: 138 RVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDI 197
           +VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  D +AI ++  ++  I
Sbjct: 257 KVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 316

Query: 198 TVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTK 257
            V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +   LG+ ++F G SL        
Sbjct: 317 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD 376

Query: 258 KLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGA 317
           KL P I A +A SN  + N   LC  G+L P+KVKGKIV+C RG NAR++KG VV  AG 
Sbjct: 377 KLLPFIYAGNA-SNATNGN---LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 436

Query: 318 VGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKP 377
           VGMILAN   NG+   ADAHLLPA+ +    G+ +  Y+ +   P A ++ + T +G+KP
Sbjct: 437 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 496

Query: 378 APIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTS 437
           +P++A+FSSRGPN+I P+ILKPD+IAPGVNI+AA++ A   TGL  D R V+F  +SGTS
Sbjct: 497 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 556

Query: 438 MSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEK-ANPLAYGAG 497
           MSCPH+SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAG
Sbjct: 557 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 616

Query: 498 HVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVC--SKSFKVIDLNYP 557
           HV P  A NPGL+YDL+ +DYL FLCA  Y   Q++  +   ++ C  SKS+ V DLNYP
Sbjct: 617 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYP 676

Query: 558 SISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVLVAPSTLKFTKTGEEKNFKV 617
           S ++           R V +VG  GTY  KV S   GV + V P+ L F +  E+K++ V
Sbjct: 677 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 736

Query: 618 VLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVT 645
                 S  +  + FG + WSDGKH V SP+ ++
Sbjct: 737 TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh16G009990 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 522.3 bits (1344), Expect = 7.7e-147
Identity = 323/783 (41.25%), Postives = 421/783 (53.77%), Query Frame = 0

Query: 4   FNLPPLLLSFFLFALLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVL 63
           F L  +L SFFLF  L  +   +KK YIVY+G+HSHG +P++ DL+LAT +HY LL S+ 
Sbjct: 6   FCLHLILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIF 65

Query: 64  G----------------------------------------------------------- 123
           G                                                           
Sbjct: 66  GSREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLG 125

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 126 LHRRGQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGS 185

Query: 184 ------RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGN 243
                 RKLIGARY+NK + ++ G L+    TARD  GHGTHTLSTAGGNFV    +F  
Sbjct: 186 MKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAV 245

Query: 244 GYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEF 303
           G GTAKGGSP+ARVAAY+VCW       C+ AD+LA  + AI DGVDV+++S G   +  
Sbjct: 246 GNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVT 305

Query: 304 SE----DLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYV 363
           +E    D ++I ++H +  +I +V SAGN GP  GTV+NVAPW+ T+ AST+DR F+S +
Sbjct: 306 AEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNL 365

Query: 364 ALGDKRHFKGKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIV 423
            + ++   +G SL    LP  + + LI + DAK  N +   A LCR+G+LD  KV GKIV
Sbjct: 366 TINNQL-IEGASLFVN-LPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIV 425

Query: 424 VCLR-GDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQY 483
           +C R G    + +G     AGA GMIL N  +NG    A+ H+    +      +S    
Sbjct: 426 LCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHG 485

Query: 484 IQST---------KT--PMAYMTHMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPG 543
           +++T         KT   +      T  G KPAP+MASFSSRGPN I+PSILKPD+ APG
Sbjct: 486 VKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPG 545

Query: 544 VNIIAAFSEATSITGLPYD-KRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRS 603
           VNI+AA+SE  S + L  D +R  +F  L GTSMSCPH SGI GLLKT +P WSPAAI+S
Sbjct: 546 VNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKS 605

Query: 604 AIMTTAGTEANDLNPILSS-EKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCA 644
           AIMTTA T  N   PI  + +K  A+  AYG+GHV+P+ A  PGLVYDLS+ DYLNFLCA
Sbjct: 606 AIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 665

BLAST of CmaCh16G009990 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 6.3e-133
Identity = 270/586 (46.08%), Postives = 368/586 (62.80%), Query Frame = 0

Query: 65  RKLIGARYFNKGY-ASNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYG 124
           +KLIGAR F+KG+  ++ G  +S  E+   RD DGHGTHT +TA G+ V+  S  G   G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 125 TAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSED 184
           TA+G + +ARVA Y+VCW    + GCF +DILA  + AI DGVDVLS+SLGGG+  +  D
Sbjct: 233 TARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRD 292

Query: 185 LLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRH 244
            +AI ++  ++  + V CSAGNSGP   +V+NVAPW++TVGA T+DR F ++  LG+ + 
Sbjct: 293 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 352

Query: 245 FKGKSLSSKL-LPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGD 304
             G SL S + + TK L      +  K N+ S N   LC  GSLD   V+GKIVVC RG 
Sbjct: 353 LTGVSLYSGVGMGTKPL----ELVYNKGNSSSSN---LCLPGSLDSSIVRGKIVVCDRGV 412

Query: 305 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTP 364
           NAR+EKG VV  AG +GMI+AN   +G+   AD+HLLPA  +    G+ + +Y++S   P
Sbjct: 413 NARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKP 472

Query: 365 MAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLP 424
            A +    T L +KP+P++A+FSSRGPNT+ P ILKPD+I PGVNI+A +S+A   TGL 
Sbjct: 473 TALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLD 532

Query: 425 YDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPIL- 484
            D R  QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA    N   P+  
Sbjct: 533 KDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD 592

Query: 485 SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFV 544
           +++   +NP A+G+GHV P KA +PGLVYD+S ++Y+ FLC+  Y    +       S  
Sbjct: 593 AADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 652

Query: 545 CSKSFK-VIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT-YVAKVESPPGVSVLVAPST 604
           CSK F     LNYPS S+      VV   R V NVG+  + Y   V   P V + V PS 
Sbjct: 653 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 712

Query: 605 LKFTKTGEEKNFKVV-LRRVQSNQTEEHVFGKLVWSDGKHRVSSPI 642
           L F   GE+K + V  + +   + T +  FG + WS+ +H V SP+
Sbjct: 713 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh16G009990 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 675.6 bits (1742), Expect = 3.9e-194
Identity = 353/583 (60.55%), Postives = 423/583 (72.56%), Query Frame = 0

Query: 65  RKLIGARYFNKGYASNVG-PLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTA 124
           RKLIGARYFNKGY +  G P N+SYET RD DGHG+HTLSTA GNFV   ++FG G GTA
Sbjct: 197 RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTA 256

Query: 125 KGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLL 184
            GGSPKARVAAY+VCWP V    CF ADILA  EAAI DGVDVLS S+GG A ++  D +
Sbjct: 257 SGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGI 316

Query: 185 AIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFK 244
           AI S+H VKN +TVVCSAGNSGP +GTVSNVAPW+ITVGAS++DR F ++V L + + FK
Sbjct: 317 AIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFK 376

Query: 245 GKSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNAR 304
           G SL SK LP +K+Y LI A DA   N +   A+LC+KGSLDPKKVKGKI+VCLRGDNAR
Sbjct: 377 GTSL-SKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 436

Query: 305 IEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAY 364
           ++KG   A AGA GM+L NDK +G+   +DAH+LPAS I Y DGE+++ Y+ STK P  Y
Sbjct: 437 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 496

Query: 365 MTHMT-ELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDK 424
           +   T  L  KPAP MASFSSRGPNTI P ILKPDI APGVNIIAAF+EAT  T L  D 
Sbjct: 497 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 556

Query: 425 RMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEK 484
           R   F   SGTSMSCPHISG+VGLLKTL+P WSPAAIRSAIMTT+ T  N   P++    
Sbjct: 557 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 616

Query: 485 EKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKS 544
           +KANP +YG+GHVQPNKAA+PGLVYDL+  DYL+FLCA GYN   ++LF  D  + C + 
Sbjct: 617 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 676

Query: 545 FKVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKT 604
             ++D NYPSI++ NL    + + R++KNVG P TY A+   P GV V V P  L F KT
Sbjct: 677 ANLLDFNYPSITVPNLTGS-ITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKT 736

Query: 605 GEEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           GE K F++ LR +    +  +VFG+L W+D  H V SPI V L
Sbjct: 737 GEVKIFQMTLRPLPVTPS-GYVFGELTWTDSHHYVRSPIVVQL 776


HSP 2 Score: 38.9 bits (89), Expect = 1.8e-02
Identity = 27/64 (42.19%), Postives = 33/64 (51.56%), Query Frame = 0

Query: 1  MEAFNLPPLLLSFFLFALLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLE 60
          M   +L  LLL   L  L  +   A KKSYIVYLGSH+H    S+  L     +H T L 
Sbjct: 16 MSLQSLSSLLL---LVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLA 75

Query: 61 SVLG 65
          S +G
Sbjct: 76 SFVG 76

BLAST of CmaCh16G009990 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 667.5 bits (1721), Expect = 1.1e-191
Identity = 339/582 (58.25%), Postives = 422/582 (72.51%), Query Frame = 0

Query: 65  RKLIGARYFNKGYASNVGPLNSSYETARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAK 124
           RKLIGARYFNKGYA+ VG LNSS+++ RD DGHG+HTLSTA G+FV  VSIFG G GTAK
Sbjct: 191 RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAK 250

Query: 125 GGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLA 184
           GGSP+ARVAAY+VCWP V    C+ AD+LA F+AAI DG DV+S+SLGG    F  D +A
Sbjct: 251 GGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVA 310

Query: 185 IMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKG 244
           I S+H  K  I VVCSAGNSGP   TVSNVAPW ITVGAST+DR F S + LG+ +H+KG
Sbjct: 311 IGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

Query: 245 KSLSSKLLPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARI 304
           +SLSS  LP  K YP++ +++AK+ N S   A LC+ GSLDP K KGKI+VCLRG N R+
Sbjct: 371 QSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRV 430

Query: 305 EKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYM 364
           EKG  VA  G +GM+L N    G+   AD H+LPA+ ++  D  +V +YI  TK P+A++
Sbjct: 431 EKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHI 490

Query: 365 T-HMTELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKR 424
           T   T+LG+KPAP+MASFSS+GP+ + P ILKPDI APGV++IAA++ A S T   +D R
Sbjct: 491 TPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPR 550

Query: 425 MVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKE 484
            + F  +SGTSMSCPHISGI GLLKT YP WSPAAIRSAIMTTA    +   PI ++   
Sbjct: 551 RLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM 610

Query: 485 KANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVCSKSF 544
           KA P ++GAGHVQPN A NPGLVYDL I+DYLNFLC+ GYN +Q+ +F+ +     S   
Sbjct: 611 KATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKI 670

Query: 545 KVIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGTYVAKVESPPGVSVLVAPSTLKFTKTG 604
            +++LNYPSI++ NL S  V + R VKNVG P  Y  KV +P GV V V P++L FTK G
Sbjct: 671 SLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVG 730

Query: 605 EEKNFKVVLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVTL 646
           E+K FKV+L + + N  + +VFG+LVWSD KHRV SPI V L
Sbjct: 731 EQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of CmaCh16G009990 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 523.1 bits (1346), Expect = 3.2e-148
Identity = 297/634 (46.85%), Postives = 402/634 (63.41%), Query Frame = 0

Query: 18  LLQTSTLAAKKSYIVYLGSHSHGLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGY 77
           +L T      KSY         G  PS+        T++T   S+  RKLIGAR+F +GY
Sbjct: 137 VLDTGVWPESKSY----SDEGFGPIPSSWKGGCEAGTNFT--ASLCNRKLIGARFFARGY 196

Query: 78  ASNVGPLNSSYE--TARDDDGHGTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAY 137
            S +GP++ S E  + RDDDGHGTHT STA G+ V+  S+ G   GTA+G +P+ARVA Y
Sbjct: 197 ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVY 256

Query: 138 RVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDI 197
           +VCW     GGCF +DILA  + AI+D V+VLS+SLGGG  ++  D +AI ++  ++  I
Sbjct: 257 KVCW----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 316

Query: 198 TVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRHFKGKSLSSKLLPTK 257
            V CSAGN+GP + ++SNVAPW+ TVGA T+DR F +   LG+ ++F G SL        
Sbjct: 317 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD 376

Query: 258 KLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGA 317
           KL P I A +A SN  + N   LC  G+L P+KVKGKIV+C RG NAR++KG VV  AG 
Sbjct: 377 KLLPFIYAGNA-SNATNGN---LCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGG 436

Query: 318 VGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKP 377
           VGMILAN   NG+   ADAHLLPA+ +    G+ +  Y+ +   P A ++ + T +G+KP
Sbjct: 437 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 496

Query: 378 APIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTS 437
           +P++A+FSSRGPN+I P+ILKPD+IAPGVNI+AA++ A   TGL  D R V+F  +SGTS
Sbjct: 497 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTS 556

Query: 438 MSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPILSSEKEK-ANPLAYGAG 497
           MSCPH+SG+  LLK+++P+WSPAAIRSA+MTTA     D  P+L     K + P  +GAG
Sbjct: 557 MSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAG 616

Query: 498 HVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFVC--SKSFKVIDLNYP 557
           HV P  A NPGL+YDL+ +DYL FLCA  Y   Q++  +   ++ C  SKS+ V DLNYP
Sbjct: 617 HVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSR-RNYTCDPSKSYSVADLNYP 676

Query: 558 SISIYNLKSEVVNIKRRVKNVGSPGTYVAKVES-PPGVSVLVAPSTLKFTKTGEEKNFKV 617
           S ++           R V +VG  GTY  KV S   GV + V P+ L F +  E+K++ V
Sbjct: 677 SFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTV 736

Query: 618 VLRRVQSNQTEEHVFGKLVWSDGKHRVSSPIFVT 645
                 S  +  + FG + WSDGKH V SP+ ++
Sbjct: 737 TFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of CmaCh16G009990 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 476.1 bits (1224), Expect = 4.5e-134
Identity = 270/586 (46.08%), Postives = 368/586 (62.80%), Query Frame = 0

Query: 65  RKLIGARYFNKGY-ASNVGPLNSSYETA--RDDDGHGTHTLSTAGGNFVQRVSIFGNGYG 124
           +KLIGAR F+KG+  ++ G  +S  E+   RD DGHGTHT +TA G+ V+  S  G   G
Sbjct: 173 KKLIGARSFSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAG 232

Query: 125 TAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFEAAISDGVDVLSISLGGGAIEFSED 184
           TA+G + +ARVA Y+VCW    + GCF +DILA  + AI DGVDVLS+SLGGG+  +  D
Sbjct: 233 TARGMATRARVATYKVCW----STGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRD 292

Query: 185 LLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPWMITVGASTVDRLFTSYVALGDKRH 244
            +AI ++  ++  + V CSAGNSGP   +V+NVAPW++TVGA T+DR F ++  LG+ + 
Sbjct: 293 TIAIGAFSAMERGVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKR 352

Query: 245 FKGKSLSSKL-LPTKKLYPLIRALDAKSNNVSDNYAILCRKGSLDPKKVKGKIVVCLRGD 304
             G SL S + + TK L      +  K N+ S N   LC  GSLD   V+GKIVVC RG 
Sbjct: 353 LTGVSLYSGVGMGTKPL----ELVYNKGNSSSSN---LCLPGSLDSSIVRGKIVVCDRGV 412

Query: 305 NARIEKGYVVAQAGAVGMILANDKENGDAFEADAHLLPASHISYTDGESVYQYIQSTKTP 364
           NAR+EKG VV  AG +GMI+AN   +G+   AD+HLLPA  +    G+ + +Y++S   P
Sbjct: 413 NARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKP 472

Query: 365 MAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSILKPDIIAPGVNIIAAFSEATSITGLP 424
            A +    T L +KP+P++A+FSSRGPNT+ P ILKPD+I PGVNI+A +S+A   TGL 
Sbjct: 473 TALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLD 532

Query: 425 YDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTEANDLNPIL- 484
            D R  QF  +SGTSMSCPHISG+ GLLK  +P+WSP+AI+SA+MTTA    N   P+  
Sbjct: 533 KDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHD 592

Query: 485 SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQDYLNFLCARGYNKAQMKLFTNDTSFV 544
           +++   +NP A+G+GHV P KA +PGLVYD+S ++Y+ FLC+  Y    +       S  
Sbjct: 593 AADNSLSNPYAHGSGHVDPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVN 652

Query: 545 CSKSFK-VIDLNYPSISIYNLKSEVVNIKRRVKNVGSPGT-YVAKVESPPGVSVLVAPST 604
           CSK F     LNYPS S+      VV   R V NVG+  + Y   V   P V + V PS 
Sbjct: 653 CSKKFSDPGQLNYPSFSVLFGGKRVVRYTREVTNVGAASSVYKVTVNGAPSVGISVKPSK 712

Query: 605 LKFTKTGEEKNFKVV-LRRVQSNQTEEHVFGKLVWSDGKHRVSSPI 642
           L F   GE+K + V  + +   + T +  FG + WS+ +H V SP+
Sbjct: 713 LSFKSVGEKKRYTVTFVSKKGVSMTNKAEFGSITWSNPQHEVRSPV 747

BLAST of CmaCh16G009990 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 469.9 bits (1208), Expect = 3.2e-132
Identity = 263/618 (42.56%), Postives = 381/618 (61.65%), Query Frame = 0

Query: 40  GLNPSAVDLQLATQTHYTLLESVLGRKLIGARYFNKGYASNVGPLNS--SYETARDDDGH 99
           G++P     + A +T    L+    RK++GAR F +GY +  G ++    Y++ RD DGH
Sbjct: 168 GMSPVPATWRGACETGKRFLKRNCNRKIVGARVFYRGYEAATGKIDEELEYKSPRDRDGH 227

Query: 100 GTHTLSTAGGNFVQRVSIFGNGYGTAKGGSPKARVAAYRVCWPEVANGGCFMADILAGFE 159
           GTHT +T  G+ V+  ++FG  YGTA+G + KARVAAY+VCW     GGCF +DIL+  +
Sbjct: 228 GTHTAATVAGSPVKGANLFGFAYGTARGMAQKARVAAYKVCWV----GGCFSSDILSAVD 287

Query: 160 AAISDGVDVLSISLGGGAIEFSEDLLAIMSYHTVKNDITVVCSAGNSGPFAGTVSNVAPW 219
            A++DGV VLSISLGGG   +S D L+I ++  ++  + V CSAGN GP   +++NV+PW
Sbjct: 288 QAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGVFVSCSAGNGGPDPISLTNVSPW 347

Query: 220 MITVGASTVDRLFTSYVALGDKRHFKGKSL--SSKLLPTKKLYPLIRALDAKSNNVSDNY 279
           + TVGAST+DR F + V +G  R FKG SL     +LP  K YPL+       N  S + 
Sbjct: 348 ITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLPKNKQYPLVY---LGRNASSPDP 407

Query: 280 AILCRKGSLDPKKVKGKIVVCLRGDNARIEKGYVVAQAGAVGMILANDKENGDAFEADAH 339
              C  G+LD + V GKIV+C RG   R++KG VV +AG +GM+L N   NG+   AD+H
Sbjct: 408 TSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGIGMVLTNTATNGEELVADSH 467

Query: 340 LLPASHISYTDGESVYQYIQSTKTPMAYMTHM-TELGIKPAPIMASFSSRGPNTIEPSIL 399
           +LPA  +   +G+ + QY  ++K   A +  + T +GIKP+P++A+FSSRGPN +   IL
Sbjct: 468 MLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPSPVVAAFSSRGPNFLSLEIL 527

Query: 400 KPDIIAPGVNIIAAFSEATSITGLPYDKRMVQFTELSGTSMSCPHISGIVGLLKTLYPKW 459
           KPD++APGVNI+AA++   + + L  D R V+F  LSGTSMSCPH+SG+  L+K+ +P W
Sbjct: 528 KPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSMSCPHVSGVAALIKSRHPDW 587

Query: 460 SPAAIRSAIMTTAGTEANDLNPIL-SSEKEKANPLAYGAGHVQPNKAANPGLVYDLSIQD 519
           SPAAI+SA+MTTA    N   P+  +S    ++P  +GAGH+ P +A +PGLVYD+  Q+
Sbjct: 588 SPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGHIDPLRATDPGLVYDIGPQE 647

Query: 520 YLNFLCARGYNKAQMKLFTNDTSFVCSKSF--KVIDLNYPSISIY---NLKSEVVNIKRR 579
           Y  FLC +  + +Q+K+FT  ++  C  +      +LNYP+IS     N   + + ++R 
Sbjct: 648 YFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPAISALFPENTHVKAMTLRRT 707

Query: 580 VKNVGSP-GTYVAKVESPPGVSVLVAPSTLKFTKTGEEKNFKVVLR-RVQSNQTEEHVFG 639
           V NVG    +Y   V    G SV V P TL FT   ++ ++ V  R R +  + E   FG
Sbjct: 708 VTNVGPHISSYKVSVSPFKGASVTVQPKTLNFTSKHQKLSYTVTFRTRFRMKRPE---FG 767

Query: 640 KLVWSDGKHRVSSPIFVT 645
            LVW    H+V SP+ +T
Sbjct: 768 GLVWKSTTHKVRSPVIIT 775

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JXC55.5e-19360.55Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
Q9ZSP51.5e-19058.25Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
O653514.5e-14746.85Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
I1N4627.7e-14741.25Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
Q9ZUF66.3e-13346.08Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 S... [more]
Match NameE-valueIdentityDescription
AT5G59810.13.9e-19460.55Subtilase family protein [more]
AT2G04160.11.1e-19158.25Subtilisin-like serine endopeptidase family protein [more]
AT5G67360.13.2e-14846.85Subtilase family protein [more]
AT2G05920.14.5e-13446.08Subtilase family protein [more]
AT5G51750.13.2e-13242.56subtilase 1.3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 431..447
score: 50.29
coord: 93..106
score: 40.76
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 63..514
e-value: 3.3E-160
score: 535.9
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 64..505
NoneNo IPR availableGENE3D2.60.40.2310coord: 518..645
e-value: 1.9E-36
score: 126.7
NoneNo IPR availableGENE3D3.50.30.30coord: 227..371
e-value: 3.3E-160
score: 535.9
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 65..645
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 1..501
score: 23.78561
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 233..362
e-value: 4.86392E-38
score: 135.234
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 286..360
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 547..643
e-value: 4.7E-28
score: 97.3
IPR003137PA domainPFAMPF02225PAcoord: 275..349
e-value: 1.3E-12
score: 47.7
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 69..493
e-value: 2.8E-40
score: 138.8
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 65..645
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 432..442

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009990.1CmaCh16G009990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity