CmaCh16G009900 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009900
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	subtilisin-like protease SBT5.4
Location	Cma_Chr16: 7626761 .. 7628889 (-)
RNA-Seq Expression	CmaCh16G009900
Synteny	CmaCh16G009900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCGATCAGCGCGGAGCCAAAACAGGTAACGACGAATTGCAATTCACCGACCTCGAAATCCGCGCAGCCGGAGTTGGAACTAGACGATCAGAAGAAGAACGCGCAGATGACGAGGTCGCCGAGCGCTAAATCGACCTGCCTCTGCTCTCCGACGACGCACGTTGGATCTTTCCGGTGACGGCGTCACCGGGGCACTGCCATCTCCCGCGGCGGCTCCGAGTGATCCTACCTCTCCGATCTGGCTCAGAAATCGGCGGCTGTGGAGGATTCAATGGAAGGTGAGTAGATTCATATGTTTGGGAGTGGTGTTTGTTAGACGAACACGACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTCTCATGGCGCCTCCCCTTAATCGAGGCTCGACTCCTTTGGAGTCTTAGTCATTTTTGACTGCCTTCGTACCTTCGAGGAGGCTTGACTCCTTTTGGAGTCCTTTGTTCGACATTTAAGGATTTGTCAATCTATTGACACGGCTAAGTTTAGGGCATGGCTCTGATACCATGTTAGACGAACACGACTCTCCACAATGGTATGATATTGTCCACATAAGCTCTCATGGCTTTGCTTTGGGCTTCCTAAAAGGCCTCACACCAATGGAGAGTGTATTCTTAGATTATAAACTCATGACCATTCCCTAAATTAACCGATGTGGGACTTTCATCATCCAACAGTGTTGAGGTTTGTGGGGGAAACTTCAATTGGTTTTCTGGTAAAACTGATTTTATGAATATTTAATGGAGAATTAACTCTATGAAAACCTTATAATAAAATAAACTTTAATCTTGCTCGCTTCATTTGTTACTTTTTGAATAAAACTAATACAAATACAAATAGCATCAAACAAAAATAATACAAAATGAAATGCTCGGCTAAAAATTAAATATGAATTTATCCTAACCGATGAGCTGTCTTGCCTTTATTTGAAAAATAGAGTAGCACATGATTGGAGTTTTTTTTAAGAAATACTAGTAATGACTAGTTATCTGGGTTATAATGCAAAGACATACCATATGTCGAGGTTGTATTTCATGCTAGTTTTTGTTTTGGAACTCCCGACAACACAAGGAGCAACTTACTCAAGTTTAGGGAGAACGCCGGTTAAGACCAACTAAGTAAGTGGCCTTACTATCGGTGTGATTATATTTGATGATTGACACGTGCCTCGTGGTTCTGCACGGGTCATATATATTCAATATGTGTGAATTGTTTATGAATTGATATGATTATGATATAATATGTATATATTATGATATGTGAATGATATCCCCCTAAAGTGTTTGTTGCTGCCTATAAAGTATGTTGGCTTCCCGTGGCAATTGATGGGTGCTTTGAGTTCAACATTCTGGCTGGCTTTGATGCTGCCATTAGTGATGGAGTTGACGTTCTGCCGATGGAGGTCCTCTAGAATTTTATGATGATCCAATAGCTATAGCATCCTTCCATGCAATGAAGAACGAATCACTGTTGTTTGTTATGTTGGAAACTCAGGACCATCTCCCATCTTTAGGCATTGTTGAAAATGTTGCACCATGGATGATAACCGTGGGAGCTAGCACAACTGACAGACTTTTTACCACTTACATGGTGCTCGGAGACAAGAGACACATTAAGGTTCTCTTTTAAGAAACACAAACACCTTAATTTAACTAACATGTTTATGTCTGACATTTGTTGGACATGTATTAACACTTGTTAACGCTAGATAATTATGTATCTAACGATGTTTATATAAATTCTAGACACATATTGAACACTTATTAAGCATGCTATATATTGAAGGGTCACTTGGGAAAATTGTGGTTGGTCTTAGAGGGTGCAGTGCAAATGTGGACAAAAGTTATGTGACAGCTCAAGCAAGTGCGGTTGGAATGATTCAGCGAATTTCTACATCCAAGACTATGACGAACTTTTCACCCATTTTCAATTCTGTATCCACCTGTGATGGCGCAAAACATTTCAACCTCATGAATTCTCTTGCATAAGCATCGACAAAACGTCCATTTTGTGTCAGGTGGGTGAACTCCCGTTGTCTTTGGAGCCAAGCTGTCTTCAAATAATATTGTTTTGAGAACACCTCTTTAAACCGAG

mRNA sequence

ATGGAACCGATCAGCGCGGAGCCAAAACAGGTAACGACGAATTGCAATTCACCGACCTCGAAATCCGCGCAGCCGGAGTTGGAACTAGACGATCAGAAGAAGAACGCGCAGATGACGAGGTCGCCGAGCGCTAAATCGACCTGCCTCTGCTCTCCGACGACGCACAAATCGGCGGCTGTGGAGGATTCAATGGAAGTATGTTGGCTTCCCGTGGCAATTGATGGGTGCTTTGAGTTCAACATTCTGGCTGGCTTTGATGCTGCCATTAGTGATGGAGTTGACGTTCTGCCGATGGAGGACCATCTCCCATCTTTAGGCATTGTTGAAAATGTTGCACCATGGATGATAACCGTGGGAGCTAGCACAACTGACAGACTTTTTACCACTTACATGGTGCTCGGAGACAAGAGACACATTAAGGGTCACTTGGGAAAATTGTGGTTGGTCTTAGAGGGTGCAGTGCAAATGTGGACAAAAGTTATGTGACAGCTCAAGCAAGTGCGGTTGGAATGATTCAGCGAATTTCTACATCCAAGACTATGACGAACTTTTCACCCATTTTCAATTCTGTATCCACCTGTGATGGCGCAAAACATTTCAACCTCATGAATTCTCTTGCATAAGCATCGACAAAACGTCCATTTTGTGTCAGGTGGGTGAACTCCCGTTGTCTTTGGAGCCAAGCTGTCTTCAAATAATATTGTTTTGAGAACACCTCTTTAAACCGAG

Coding sequence (CDS)

ATGGAACCGATCAGCGCGGAGCCAAAACAGGTAACGACGAATTGCAATTCACCGACCTCGAAATCCGCGCAGCCGGAGTTGGAACTAGACGATCAGAAGAAGAACGCGCAGATGACGAGGTCGCCGAGCGCTAAATCGACCTGCCTCTGCTCTCCGACGACGCACAAATCGGCGGCTGTGGAGGATTCAATGGAAGTATGTTGGCTTCCCGTGGCAATTGATGGGTGCTTTGAGTTCAACATTCTGGCTGGCTTTGATGCTGCCATTAGTGATGGAGTTGACGTTCTGCCGATGGAGGACCATCTCCCATCTTTAGGCATTGTTGAAAATGTTGCACCATGGATGATAACCGTGGGAGCTAGCACAACTGACAGACTTTTTACCACTTACATGGTGCTCGGAGACAAGAGACACATTAAGGGTCACTTGGGAAAATTGTGGTTGGTCTTAGAGGGTGCAGTGCAAATGTGGACAAAAGTTATGTGA

Protein sequence

MEPISAEPKQVTTNCNSPTSKSAQPELELDDQKKNAQMTRSPSAKSTCLCSPTTHKSAAVEDSMEVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPMEDHLPSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKGHLGKLWLVLEGAVQMWTKVM

Homology

[Close]

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-11
Identity = 40/109 (36.70%), Postives = 54/109 (49.54%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME------------------------- 124
           +VCW PV  + C++ ++LA FDAAI DG DV+ +                          
Sbjct: 262 KVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRI 321

Query: 125 -------DHLPSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                  +  P+   V NVAPW ITVGAST DR F + +VLG+ +H KG
Sbjct: 322 VVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

[Close]

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.4e-09
Identity = 41/109 (37.61%), Postives = 52/109 (47.71%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLP----------MEDHL------------ 124
           +VCW PV    CF+ +ILA  +AAI DGVDVL           M D +            
Sbjct: 269 KVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGV 328

Query: 125 ----------PSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     P  G V NVAPW+ITVGAS+ DR F  ++ L + +  KG
Sbjct: 329 TVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 377

[Close]

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.0e-07
Identity = 37/109 (33.94%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME-------DHLPSLGI---------- 124
           +VCW      GC++ +ILA FD A++DGVDV+ +         +L ++ I          
Sbjct: 249 KVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGI 308

Query: 125 ---------------VENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                          V NVAPWM TVGA T DR F   + LG+ + I G
Sbjct: 309 FVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISG 353

[Close]

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 3.0e-07
Identity = 36/112 (32.14%), Postives = 49/112 (43.75%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME------------------------- 124
           +VCW      GC + +ILA FDAA+ DGVDV+ +                          
Sbjct: 244 KVCWKD---SGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 303

Query: 125 ----------DHLPSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     +  P+   V N+APW+ TVGAST DR F    +LGD   ++G
Sbjct: 304 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 352

[Close]

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 8.7e-07
Identity = 37/109 (33.94%), Postives = 48/109 (44.04%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLP------MEDHL---------------- 124
           +VCWL     GCF  +ILA  D AI+D V+VL       M D+                 
Sbjct: 251 KVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 310

Query: 125 ----------PSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     PS   + NVAPW+ TVGA T DR F    +LG+ ++  G
Sbjct: 311 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 355

[Close]

BLAST of CmaCh16G009900 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.1e-12
Identity = 40/109 (36.70%), Postives = 54/109 (49.54%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME------------------------- 124
           +VCW PV  + C++ ++LA FDAAI DG DV+ +                          
Sbjct: 262 KVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRI 321

Query: 125 -------DHLPSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                  +  P+   V NVAPW ITVGAST DR F + +VLG+ +H KG
Sbjct: 322 VVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKG 370

[Close]

BLAST of CmaCh16G009900 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.0e-10
Identity = 41/109 (37.61%), Postives = 52/109 (47.71%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLP----------MEDHL------------ 124
           +VCW PV    CF+ +ILA  +AAI DGVDVL           M D +            
Sbjct: 269 KVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGV 328

Query: 125 ----------PSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     P  G V NVAPW+ITVGAS+ DR F  ++ L + +  KG
Sbjct: 329 TVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 377

[Close]

BLAST of CmaCh16G009900 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.1e-08
Identity = 37/109 (33.94%), Postives = 51/109 (46.79%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME-------DHLPSLGI---------- 124
           +VCW      GC++ +ILA FD A++DGVDV+ +         +L ++ I          
Sbjct: 249 KVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGI 308

Query: 125 ---------------VENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                          V NVAPWM TVGA T DR F   + LG+ + I G
Sbjct: 309 FVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISG 353

[Close]

BLAST of CmaCh16G009900 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 56.6 bits (135), Expect = 2.1e-08
Identity = 36/112 (32.14%), Postives = 49/112 (43.75%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLPME------------------------- 124
           +VCW      GC + +ILA FDAA+ DGVDV+ +                          
Sbjct: 244 KVCWKD---SGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLDPIAIGSYGAAS 303

Query: 125 ----------DHLPSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     +  P+   V N+APW+ TVGAST DR F    +LGD   ++G
Sbjct: 304 KGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHRLRG 352

[Close]

BLAST of CmaCh16G009900 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 55.1 bits (131), Expect = 6.2e-08
Identity = 37/109 (33.94%), Postives = 48/109 (44.04%), Query Frame = 0


Query: 65  EVCWLPVAIDGCFEFNILAGFDAAISDGVDVLP------MEDHL---------------- 124
           +VCWL     GCF  +ILA  D AI+D V+VL       M D+                 
Sbjct: 251 KVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGI 310

Query: 125 ----------PSLGIVENVAPWMITVGASTTDRLFTTYMVLGDKRHIKG 142
                     PS   + NVAPW+ TVGA T DR F    +LG+ ++  G
Sbjct: 311 LVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 355

The following BLAST results are available for this feature:

BLAST of CmaCh16G009900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	1.5e-11	36.70	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	1.4e-09	37.61	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9LUM3	3.0e-07	33.94	Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S...	[more]
O49607	3.0e-07	32.14	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]
O65351	8.7e-07	33.94	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]

BLAST of CmaCh16G009900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	1.1e-12	36.70	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	1.0e-10	37.61	Subtilase family protein	[more]
AT3G14240.1	2.1e-08	33.94	Subtilase family protein	[more]
AT4G34980.1	2.1e-08	32.14	subtilisin-like serine protease 2	[more]
AT5G67360.1	6.2e-08	33.94	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 103..123 e-value: 5.8E-7 score: 30.6
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 67..137
None	No IPR available	GENE3D	3.50.30.30		coord: 124..142 e-value: 5.8E-7 score: 30.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..47
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 7..25
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 103..147 coord: 63..98
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 103..147 coord: 63..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009900.1	CmaCh16G009900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity