CmaCh16G009870 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009870
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	subtilisin-like protease SBT5.3
Location	Cma_Chr16: 7597941 .. 7599147 (-)
RNA-Seq Expression	CmaCh16G009870
Synteny	CmaCh16G009870

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTACATGACTCATGTAAGGACAGAGATGGGAATCAAACCGGCACCCGTTATGGCTTCATTCTCATCTAGAGGTCCCAATTCAATCGAGGAGTCGATACTCAAGGTTTCCATGTTTACTCTCCTTTTCGTATACACTGTGTTTCTACTTTAGAATATTCAATGTTCAACTCAAAATATTAAGTGTGTGTATCTCTTTTTTTTTTTTTTTTTTTTTTNTGTTCAACTCAAAATATTAAGTGTGTGTATTTTTTTTTTTTTTTTTTTTTTACTTCTTCAGCCTGATATAACAGCACCAGGTGTTAATATAATTGCAGCCTATTTAGAAGATGCATCACCAAGTGGTTCACCATTTGATAGTCGAATTCCATTTAATGGAGTATCTGACACTTCCATGTCGCGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACTACAGGTACATTCAAATTATCAAATTATTACGTAAATATTTGGGGCTAAGATATAAAGGATTGACTGCATTAAAGTGTTCGATATAACCAAATTTAGTGTAACCAATTAGAGCTCCACAGTGATTCGAGAAGTTATTTGTGCTCATACCCATTGTAATAACATTTCCTTCACTTCTTCAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

mRNA sequence

ATGGCTTACATGACTCATGTAAGGACAGAGATGGGAATCAAACCGGCACCCGTTATGGCTTCATTCTCATCTAGAGGTCCCAATTCAATCGAGGAGTCGATACTCAAGCCTGATATAACAGCACCAGGTGTTAATATAATTGCAGCCTATTTAGAAGATGCATCACCAAGTGGTTCACCATTTGATAGTCGAATTCCATTTAATGGAGTATCTGACACTTCCATGTCGCGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACTACAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

Coding sequence (CDS)

ATGGCTTACATGACTCATGTAAGGACAGAGATGGGAATCAAACCGGCACCCGTTATGGCTTCATTCTCATCTAGAGGTCCCAATTCAATCGAGGAGTCGATACTCAAGCCTGATATAACAGCACCAGGTGTTAATATAATTGCAGCCTATTTAGAAGATGCATCACCAAGTGGTTCACCATTTGATAGTCGAATTCCATTTAATGGAGTATCTGACACTTCCATGTCGCGTCCGCATATTTCTGGTATTGTTGCCCTTCTCAAGACCATTTATCCAAAATGGAGTCCAGCAGCTATCAAATCTGCAATCATGACTACAGCTGAAACTAGAGCCAATGACTTGCATCCAATACTAGATTTCACCAAGCTTGTAGCCAACCCATTGGCGTACGGTGCGGGACATGTGCAACCCAACCGAGCAGCAAATCCTGGCCTTGTTTATGACCTTACCACCAATGACTATTTAAATTTTTTATGTGCTCGAGGCTACAATAAAGCACAACTCAGTAAATTCTCCAATACGTCATTCGTTTGTTCAAAGTCATTCAAACTAACAGATTTCAACTACCCATCAATCTCGATACCCAATATGAAATCAGGACCTGTGACAATCAAAAGAACAGTTAAGAATGTGGGAAGCCCAAGTACATATGTAGCTCGGGTGGAGGTACCCCCAGGAGTTTTAGTTTCAATTAAGCCAAGTACGTTGAAGTTTACTCGAACTGATGAAGAGAAGACTTTCGAAGTTGTATTCCAAAGTGTGGCAAACAACAAGCATCGAGGCTATGTATTTGGGTCTTTGAAATGGTTAGATGGTAAGCATCATGTAAGAAGCTCAATTGTAGTGAATTTGGGATGA

Protein sequence

MAYMTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDSRIPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNLG

Homology

[Close]

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.0e-87
Identity = 163/286 (56.99%), Postives = 202/286 (70.63%), Query Frame = 0


Query: 1   MAYMTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSP 60
           +A++T  RT++G+KPAPVMASFSS+GP+ +   ILKPDITAPGV++IAAY    SP+   
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 546

Query: 61  FD-SRIPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD 120
           FD  R+ FN +S TSMS PHISGI  LLKT YP WSPAAI+SAIMTTA    +   PI +
Sbjct: 547 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 606

Query: 121 FTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCS 180
            T + A P ++GAGHVQPN A NPGLVYDL   DYLNFLC+ GYN +Q+S FS  +F CS
Sbjct: 607 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 666

Query: 181 K-SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKF 240
                L + NYPSI++PN+ S  VT+ RTVKNVG PS Y  +V  P GV V++KP++L F
Sbjct: 667 SPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 726

Query: 241 TRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNL 285
           T+  E+KTF+V+      N  +GYVFG L W D KH VRS IVV L
Sbjct: 727 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 301.6 bits (771), Expect = 9.4e-81
Identity = 160/283 (56.54%), Postives = 194/283 (68.55%), Query Frame = 0


Query: 4   MTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDS 63
           M+  RT  G KPAPVMASFSSRGPN I+ SILKPD+TAPGVNI+AAY E AS S    D+
Sbjct: 504 MSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDN 563

Query: 64  R--IPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD-F 123
           R    FN +  TSMS PH SGI  LLKT +P WSPAAIKSAIMTTA T  N   PI D F
Sbjct: 564 RRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAF 623

Query: 124 TKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFS-NTSFVCS 183
            K +A+  AYG+GHV+P+ A  PGLVYDL+  DYLNFLCA GY++  +S  + N +F+CS
Sbjct: 624 DKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICS 683

Query: 184 KSFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFT 243
            S  + D NYPSI++PN++  PVTI RTV NVG PSTY      P G  +++ P +L FT
Sbjct: 684 GSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFT 743

Query: 244 RTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVV 283
           +  E KTF+V+ Q+ +    R Y FG L+W DGKH VRS I V
Sbjct: 744 KIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

[Close]

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 6.1e-80
Identity = 156/284 (54.93%), Postives = 196/284 (69.01%), Query Frame = 0


Query: 3   YMTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFD 62
           Y+      +  KPAP MASFSSRGPN+I   ILKPDITAPGVNIIAA+ E   P+    D
Sbjct: 495 YIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSD 554

Query: 63  S-RIPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFT 122
           + R PFN  S TSMS PHISG+V LLKT++P WSPAAI+SAIMTT+ TR N   P++D +
Sbjct: 555 NRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES 614

Query: 123 KLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFS-NTSFVCSK 182
              ANP +YG+GHVQPN+AA+PGLVYDLTT DYL+FLCA GYN   +  F+ +  + C +
Sbjct: 615 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 674

Query: 183 SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTR 242
              L DFNYPSI++PN+ +G +T+ R +KNVG P+TY AR   P GV VS++P  L F +
Sbjct: 675 GANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 734

Query: 243 TDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNL 285
           T E K F++  + +      GYVFG L W D  H+VRS IVV L
Sbjct: 735 TGEVKIFQMTLRPLPVTP-SGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 1.7e-66
Identity = 133/280 (47.50%), Postives = 181/280 (64.64%), Query Frame = 0


Query: 9   TEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDS-RIPF 68
           T +G+KP+PV+A+FSSRGPNSI  +ILKPD+ APGVNI+AA+   A P+G   DS R+ F
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 535

Query: 69  NGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDF-TKLVAN 128
           N +S TSMS PH+SG+ ALLK+++P+WSPAAI+SA+MTTA     D  P+LD  T   + 
Sbjct: 536 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 595

Query: 129 PLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC--SKSFKL 188
           P  +GAGHV P  A NPGL+YDLTT DYL FLCA  Y   Q+   S  ++ C  SKS+ +
Sbjct: 596 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 655

Query: 189 TDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARV-EVPPGVLVSIKPSTLKFTRTDE 248
            D NYPS ++     G     RTV +VG   TY  +V     GV +S++P+ L F   +E
Sbjct: 656 ADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANE 715

Query: 249 EKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVN 284
           +K++ V F   ++       FGS++W DGKH V S + ++
Sbjct: 716 KKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 1.9e-57
Identity = 133/287 (46.34%), Postives = 174/287 (60.63%), Query Frame = 0


Query: 9   TEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDSR-IPF 68
           T +GIKPAPV+ASFS RGPN +   ILKPD+ APGVNI+AA+ +   P+G P D R   F
Sbjct: 472 TIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF 531

Query: 69  NGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD-FTKLVAN 128
           N +S TSM+ PH+SG  ALLK+ +P WSPA I+SA+MTT     N    ++D  T   A 
Sbjct: 532 NILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSAT 591

Query: 129 PLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLT- 188
           P  YG+GH+   RA NPGLVYD+T +DY+ FLC+ GY    +   + T   C  + K + 
Sbjct: 592 PYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSP 651

Query: 189 -DFNYPSIS--IPNMKSGPV--TIKRTVKNVG-SPSTYVARVEVPPGVLVSIKPSTLKFT 248
            + NYPSI+   P  + G V  T+ RT  NVG + + Y AR+E P GV V++KP  L FT
Sbjct: 652 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFT 711

Query: 249 RTDEEKTFEVVFQSVANN---KHRGYVFGSLKWLD-GKHHVRSSIVV 283
              + +++ V       N      G VFGS+ W D GKH VRS IVV
Sbjct: 712 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

[Close]

BLAST of CmaCh16G009870 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 322.8 bits (826), Expect = 2.8e-88
Identity = 163/286 (56.99%), Postives = 202/286 (70.63%), Query Frame = 0


Query: 1   MAYMTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSP 60
           +A++T  RT++G+KPAPVMASFSS+GP+ +   ILKPDITAPGV++IAAY    SP+   
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 546

Query: 61  FD-SRIPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD 120
           FD  R+ FN +S TSMS PHISGI  LLKT YP WSPAAI+SAIMTTA    +   PI +
Sbjct: 547 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 606

Query: 121 FTKLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCS 180
            T + A P ++GAGHVQPN A NPGLVYDL   DYLNFLC+ GYN +Q+S FS  +F CS
Sbjct: 607 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 666

Query: 181 K-SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKF 240
                L + NYPSI++PN+ S  VT+ RTVKNVG PS Y  +V  P GV V++KP++L F
Sbjct: 667 SPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 726

Query: 241 TRTDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNL 285
           T+  E+KTF+V+      N  +GYVFG L W D KH VRS IVV L
Sbjct: 727 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

[Close]

BLAST of CmaCh16G009870 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 298.9 bits (764), Expect = 4.3e-81
Identity = 156/284 (54.93%), Postives = 196/284 (69.01%), Query Frame = 0


Query: 3   YMTHVRTEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFD 62
           Y+      +  KPAP MASFSSRGPN+I   ILKPDITAPGVNIIAA+ E   P+    D
Sbjct: 495 YIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSD 554

Query: 63  S-RIPFNGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDFT 122
           + R PFN  S TSMS PHISG+V LLKT++P WSPAAI+SAIMTT+ TR N   P++D +
Sbjct: 555 NRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES 614

Query: 123 KLVANPLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFS-NTSFVCSK 182
              ANP +YG+GHVQPN+AA+PGLVYDLTT DYL+FLCA GYN   +  F+ +  + C +
Sbjct: 615 FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQ 674

Query: 183 SFKLTDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARVEVPPGVLVSIKPSTLKFTR 242
              L DFNYPSI++PN+ +G +T+ R +KNVG P+TY AR   P GV VS++P  L F +
Sbjct: 675 GANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 734

Query: 243 TDEEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVNL 285
           T E K F++  + +      GYVFG L W D  H+VRS IVV L
Sbjct: 735 TGEVKIFQMTLRPLPVTP-SGYVFGELTWTDSHHYVRSPIVVQL 776

[Close]

BLAST of CmaCh16G009870 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 254.2 bits (648), Expect = 1.2e-67
Identity = 133/280 (47.50%), Postives = 181/280 (64.64%), Query Frame = 0


Query: 9   TEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDS-RIPF 68
           T +G+KP+PV+A+FSSRGPNSI  +ILKPD+ APGVNI+AA+   A P+G   DS R+ F
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEF 535

Query: 69  NGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDF-TKLVAN 128
           N +S TSMS PH+SG+ ALLK+++P+WSPAAI+SA+MTTA     D  P+LD  T   + 
Sbjct: 536 NIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPST 595

Query: 129 PLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVC--SKSFKL 188
           P  +GAGHV P  A NPGL+YDLTT DYL FLCA  Y   Q+   S  ++ C  SKS+ +
Sbjct: 596 PFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSV 655

Query: 189 TDFNYPSISIPNMKSGPVTIKRTVKNVGSPSTYVARV-EVPPGVLVSIKPSTLKFTRTDE 248
            D NYPS ++     G     RTV +VG   TY  +V     GV +S++P+ L F   +E
Sbjct: 656 ADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANE 715

Query: 249 EKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVVN 284
           +K++ V F   ++       FGS++W DGKH V S + ++
Sbjct: 716 KKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

[Close]

BLAST of CmaCh16G009870 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-58
Identity = 133/287 (46.34%), Postives = 174/287 (60.63%), Query Frame = 0


Query: 9   TEMGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFDSR-IPF 68
           T +GIKPAPV+ASFS RGPN +   ILKPD+ APGVNI+AA+ +   P+G P D R   F
Sbjct: 472 TIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPSDPRKTEF 531

Query: 69  NGVSDTSMSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD-FTKLVAN 128
           N +S TSM+ PH+SG  ALLK+ +P WSPA I+SA+MTT     N    ++D  T   A 
Sbjct: 532 NILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDESTGKSAT 591

Query: 129 PLAYGAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLT- 188
           P  YG+GH+   RA NPGLVYD+T +DY+ FLC+ GY    +   + T   C  + K + 
Sbjct: 592 PYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCPTTRKPSP 651

Query: 189 -DFNYPSIS--IPNMKSGPV--TIKRTVKNVG-SPSTYVARVEVPPGVLVSIKPSTLKFT 248
            + NYPSI+   P  + G V  T+ RT  NVG + + Y AR+E P GV V++KP  L FT
Sbjct: 652 GNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVKPPRLVFT 711

Query: 249 RTDEEKTFEVVFQSVANN---KHRGYVFGSLKWLD-GKHHVRSSIVV 283
              + +++ V       N      G VFGS+ W D GKH VRS IVV
Sbjct: 712 SAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

[Close]

BLAST of CmaCh16G009870 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.1e-55
Identity = 125/280 (44.64%), Postives = 171/280 (61.07%), Query Frame = 0


Query: 16  APVMASFSSRGPNSIEESILKPDITAPGVNIIAAYLEDASPSGSPFD-SRIPFNGVSDTS 75
           AP++A+FSSRGP+     I KPDI APG+NI+A +   +SPS    D  R+ FN +S TS
Sbjct: 493 APMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTS 552

Query: 76  MSRPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILD----FTKLVANPLAY 135
           M+ PHISGI AL+K+++  WSPA IKSAIMTTA    N   PI D      +  A   A+
Sbjct: 553 MACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAF 612

Query: 136 GAGHVQPNRAANPGLVYDLTTNDYLNFLCARGYNKAQLSKFSNTSFVCSKSFKLT---DF 195
           GAG+V P RA +PGLVYD +T DYLN+LC+  Y   ++  FS T++ C+ +  +    D 
Sbjct: 613 GAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTCASNAVVLSPGDL 672

Query: 196 NYPSISIPNMKSG----PVTIKRTVKNVGSPS-TYVARVEVPPGVLVSIKPSTLKFTRTD 255
           NYPS ++ N+ +G     V  KRTV NVGSP+  Y+  VE P GV V ++P  LKF +  
Sbjct: 673 NYPSFAV-NLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRVEPKVLKFQKAR 732

Query: 256 EEKTFEVVFQSVANNKHRGYVFGSLKWLDGKHHVRSSIVV 283
           E  ++ V + + A+       FG L W+  K++VRS I V
Sbjct: 733 ERLSYTVTYDAEASRNSSSSSFGVLVWICDKYNVRSPIAV 771

The following BLAST results are available for this feature:

BLAST of CmaCh16G009870 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	4.0e-87	56.99	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	9.4e-81	56.54	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
F4JXC5	6.1e-80	54.93	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
O65351	1.7e-66	47.50	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]
O49607	1.9e-57	46.34	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]

BLAST of CmaCh16G009870 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	2.8e-88	56.99	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	4.3e-81	54.93	Subtilase family protein	[more]
AT5G67360.1	1.2e-67	47.50	Subtilase family protein	[more]
AT4G34980.1	1.4e-58	46.34	subtilisin-like serine protease 2	[more]
AT1G01900.1	1.1e-55	44.64	subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.40.2310		coord: 158..284 e-value: 2.9E-37 score: 129.3
None	No IPR available	PANTHER	PTHR43806:SF34	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 1..283
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 1..283
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..141 score: 12.563576
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 13..154 e-value: 5.9E-55 score: 188.8
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 16..142
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 186..282 e-value: 1.0E-28 score: 99.4
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 16..112 e-value: 2.8E-19 score: 69.5

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009870.1	CmaCh16G009870.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity