CmaCh16G009820 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009820
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Subtilisin-like protease
Location	Cma_Chr16: 7574882 .. 7575310 (-)
RNA-Seq Expression	CmaCh16G009820
Synteny	CmaCh16G009820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTATATGACTTATATGACTCGTGTGAGGACGGAATCGGGAGTCAAACCAGCACCAATTAAGGCTACATTTTCATCTAGAGGTCCCAACACAATTCAACTATTTAGTACAAAGTTTAATGTACCAATTGCCAACCATAAATTTTTTTTGAGTTTTTCATTTATCATTTTTGATTTTCAGCCTGATATAACAACACCAGGTGTAAATATATTAGCCGCTTATTCGGAAGCAACATCACCAACTGGTGCACCTTTTGATAAACGACAAGTTCCATTTATTATATTATCTGGTACTTCTATGCCATGCCCTCATATTTCTGGTATCGTTGGCCTTCTCAAAACCCTTTATCCAAAATGGAGCCCAGCAATTATGAGATCTGCAATCATGACTACAGGTTCGTTACTTGAACTTCTTCCATCATTATAA

mRNA sequence

ATGACTTATATGACTTATATGACTCGTGTGAGGACGGAATCGGGAGTCAAACCAGCACCAATTAAGGCTACATTTTCATCTAGAGGTCCCAACACAATTCAACTATTTAGTACAAAGTTTAATCCTGATATAACAACACCAGGTGTAAATATATTAGCCGCTTATTCGGAAGCAACATCACCAACTGGTGCACCTTTTGATAAACGACAAGTTCCATTTATTATATTATCTGGTACTTCTATGCCATGCCCTCATATTTCTGGTATCGTTGGCCTTCTCAAAACCCTTTATCCAAAATGGAGCCCAGCAATTATGAGATCTGCAATCATGACTACAGGTTCGTTACTTGAACTTCTTCCATCATTATAA

Coding sequence (CDS)

ATGACTTATATGACTTATATGACTCGTGTGAGGACGGAATCGGGAGTCAAACCAGCACCAATTAAGGCTACATTTTCATCTAGAGGTCCCAACACAATTCAACTATTTAGTACAAAGTTTAATCCTGATATAACAACACCAGGTGTAAATATATTAGCCGCTTATTCGGAAGCAACATCACCAACTGGTGCACCTTTTGATAAACGACAAGTTCCATTTATTATATTATCTGGTACTTCTATGCCATGCCCTCATATTTCTGGTATCGTTGGCCTTCTCAAAACCCTTTATCCAAAATGGAGCCCAGCAATTATGAGATCTGCAATCATGACTACAGGTTCGTTACTTGAACTTCTTCCATCATTATAA

Protein sequence

MTYMTYMTRVRTESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLELLPSL

Homology

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BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 9.4e-30
Identity = 64/117 (54.70%), Postives = 87/117 (74.36%), Query Frame = 0


Query: 4   MTYMTRVRTESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTG 63
           + ++T  RT+ G+KPAP+ A+FSS+GP+ +     K  PDIT PGV+++AAY+ A SPT 
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILK--PDITAPGVSVIAAYTGAVSPTN 546

Query: 64  APFDKRQVPFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLELLP 121
             FD R++ F  +SGTSM CPHISGI GLLKT YP WSPA +RSAIMTT ++++ +P
Sbjct: 547 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIP 601

[Close]

BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-28
Identity = 65/96 (67.71%), Postives = 73/96 (76.04%), Query Frame = 0


Query: 17  KPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFIIL 76
           KPAP  A+FSSRGPNTI     K  PDIT PGVNI+AA++EAT PT    D R+ PF   
Sbjct: 506 KPAPFMASFSSRGPNTITPGILK--PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTE 565

Query: 77  SGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTT 113
           SGTSM CPHISG+VGLLKTL+P WSPA +RSAIMTT
Sbjct: 566 SGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTT 599

[Close]

BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 122.1 bits (305), Expect = 4.4e-27
Identity = 65/112 (58.04%), Postives = 78/112 (69.64%), Query Frame = 0


Query: 7   MTRVRTESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPF 66
           M+R RT  G KPAP+ A+FSSRGPN IQ   +   PD+T PGVNILAAYSE  S +    
Sbjct: 504 MSRARTLFGRKPAPVMASFSSRGPNKIQ--PSILKPDVTAPGVNILAAYSEFASASSLLV 563

Query: 67  DKRQ-VPFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLE 118
           D R+   F +L GTSM CPH SGI GLLKT +P WSPA ++SAIMTT + L+
Sbjct: 564 DNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLD 613

[Close]

BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 7.5e-27
Identity = 58/103 (56.31%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 15  GVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFI 74
           G+KPAP+ A+FS RGPN +     K  PD+  PGVNILAA+++A  PTG P D R+  F 
Sbjct: 475 GIKPAPVIASFSGRGPNGLSPEILK--PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 534

Query: 75  ILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLE 118
           ILSGTSM CPH+SG   LLK+ +P WSPA++RSA+MTT +L++
Sbjct: 535 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 575

[Close]

BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 2.8e-26
Identity = 58/101 (57.43%), Postives = 74/101 (73.27%), Query Frame = 0


Query: 12  TESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQV 71
           T  GVKP+P+ A FSSRGPN+I        PD+  PGVNILAA++ A  PTG   D R+V
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSIT--PNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 535

Query: 72  PFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTT 113
            F I+SGTSM CPH+SG+  LLK+++P+WSPA +RSA+MTT
Sbjct: 536 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTT 574

[Close]

BLAST of CmaCh16G009820 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 131.0 bits (328), Expect = 6.7e-31
Identity = 64/117 (54.70%), Postives = 87/117 (74.36%), Query Frame = 0


Query: 4   MTYMTRVRTESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTG 63
           + ++T  RT+ G+KPAP+ A+FSS+GP+ +     K  PDIT PGV+++AAY+ A SPT 
Sbjct: 487 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILK--PDITAPGVSVIAAYTGAVSPTN 546

Query: 64  APFDKRQVPFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLELLP 121
             FD R++ F  +SGTSM CPHISGI GLLKT YP WSPA +RSAIMTT ++++ +P
Sbjct: 547 EQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIP 601

[Close]

BLAST of CmaCh16G009820 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 127.1 bits (318), Expect = 9.7e-30
Identity = 65/96 (67.71%), Postives = 73/96 (76.04%), Query Frame = 0


Query: 17  KPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFIIL 76
           KPAP  A+FSSRGPNTI     K  PDIT PGVNI+AA++EAT PT    D R+ PF   
Sbjct: 506 KPAPFMASFSSRGPNTITPGILK--PDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTE 565

Query: 77  SGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTT 113
           SGTSM CPHISG+VGLLKTL+P WSPA +RSAIMTT
Sbjct: 566 SGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTT 599

[Close]

BLAST of CmaCh16G009820 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 121.3 bits (303), Expect = 5.3e-28
Identity = 58/103 (56.31%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 15  GVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFI 74
           G+KPAP+ A+FS RGPN +     K  PD+  PGVNILAA+++A  PTG P D R+  F 
Sbjct: 475 GIKPAPVIASFSGRGPNGLSPEILK--PDLIAPGVNILAAWTDAVGPTGLPSDPRKTEFN 534

Query: 75  ILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLE 118
           ILSGTSM CPH+SG   LLK+ +P WSPA++RSA+MTT +L++
Sbjct: 535 ILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 575

[Close]

BLAST of CmaCh16G009820 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 119.4 bits (298), Expect = 2.0e-27
Identity = 58/101 (57.43%), Postives = 74/101 (73.27%), Query Frame = 0


Query: 12  TESGVKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQV 71
           T  GVKP+P+ A FSSRGPN+I        PD+  PGVNILAA++ A  PTG   D R+V
Sbjct: 476 TVVGVKPSPVVAAFSSRGPNSIT--PNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRV 535

Query: 72  PFIILSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTT 113
            F I+SGTSM CPH+SG+  LLK+++P+WSPA +RSA+MTT
Sbjct: 536 EFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTT 574

[Close]

BLAST of CmaCh16G009820 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 117.9 bits (294), Expect = 5.9e-27
Identity = 57/102 (55.88%), Postives = 74/102 (72.55%), Query Frame = 0


Query: 16  VKPAPIKATFSSRGPNTIQLFSTKFNPDITTPGVNILAAYSEATSPTGAPFDKRQVPFII 75
           VKP+P+ A FSSRGPNT+     K  PD+  PGVNILA +S+A  PTG   D R+  F I
Sbjct: 474 VKPSPVVAAFSSRGPNTVTPEILK--PDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNI 533

Query: 76  LSGTSMPCPHISGIVGLLKTLYPKWSPAIMRSAIMTTGSLLE 118
           +SGTSM CPHISG+ GLLK  +P+WSP+ ++SA+MTT  +L+
Sbjct: 534 MSGTSMSCPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLD 573

The following BLAST results are available for this feature:

BLAST of CmaCh16G009820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSP5	9.4e-30	54.70	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
F4JXC5	1.4e-28	67.71	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
I1N462	4.4e-27	58.04	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
O49607	7.5e-27	56.31	Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S...	[more]
O65351	2.8e-26	57.43	Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S...	[more]

BLAST of CmaCh16G009820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G04160.1	6.7e-31	54.70	Subtilisin-like serine endopeptidase family protein	[more]
AT5G59810.1	9.7e-30	67.71	Subtilase family protein	[more]
AT4G34980.1	5.3e-28	56.31	subtilisin-like serine protease 2	[more]
AT5G67360.1	2.0e-27	57.43	Subtilase family protein	[more]
AT2G05920.1	5.9e-27	55.88	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 23..112 e-value: 1.2E-15 score: 57.5
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 15..114 e-value: 1.5E-33 score: 118.3
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 19..116
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 3..113
None	No IPR available	PANTHER	PTHR10795:SF662	SUBTILISIN-LIKE PROTEASE SBT5.4	coord: 3..113
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 1..122 score: 12.156845

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009820.1	CmaCh16G009820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
biological_process	GO:0009610	response to symbiotic fungus
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity