CmaCh16G009770 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009770
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RNA-binding protein pno1-like
Location	Cma_Chr16: 7527285 .. 7528776 (-)
RNA-Seq Expression	CmaCh16G009770
Synteny	CmaCh16G009770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATTGTTAAGCCCTAGGACGCCTTGTTCTTCCACCTTCCGTCTTCACAACCTCGTTTTCTCCTCTTTTTCTTAGCAGTTCAGTTTGCATTATCCCACAGCCTCCGCCGCCGCCGCAGCACACTTCTTCACGGACGTTACACCCTCTCTTGATCTCAGGTATTCTCTTTATTTTTCTTTCTTTCAGTTTCAACTTTTATTGCTTACAAATGGATGAACCTCTGCAAGCTTTGATCATCGGTTTTGAGTTTTTCCTGATTTGAAATTGGATTATAGTATTTTAGAATCCGGTACTGTTCTGGATCAATGTTACTTATATTATAGTATTCTAGAACCAATTCATCGTTTACATGAAAATTTTTTGACATTCTGAGGAGCGCGCCACTGAAGTTTCTTCAGATTATTTACGCCTAAAATTGTGCTGAACTACATCGTATCTGGATTTTGTTGTTTAATTGTAGTTTTTGGGTGTGGTTTTCAGTGTGGTAACTCAGCACAATTCGATAATGCAGCCGACCGAAGCCTCCACTTCTATGGAAGTAGAAACGGTCTCAACCGAAACAAAGAATGTGGTTAGCACATTGCCACCGAAACCACAGTTTGATCCCCTAAAGCCACATGAGATGAATGATGGACGAATTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGCTATACGCCTCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAACAGATGAAAATCGACATCCGAATGAATCTCAAGGCGCGTAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGACATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTTCATTCTTGGGTTCGATGTTATCGATGCCATTGCCCTCCTAAGGGTGGATGAACTCTATGTTGAATCTTTTGAGATTAAGGATGTTAAAACGCTACGAGGCGAGCACTTGTCGCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACCAAATTTGCCATTGAAAACGCCACGAAGACGAGGATCGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTGATTTTAGGGTCCCCTGCAGGTAAAGTTTATTCAAAACTAAGGGCGGTTACAGCCAGATTAGCAGAAAGGTTTTGATCTTTCATGATCATAATGAGATTCATGCTGAGTTTGTCTTGATTAATGAATTTTGCATACACTAGTTGTCTGAGGAACAGAGTGTATCTTTGTCGAAAAGATTGCACATTGAAGAAGTTGTCTCATTAAGTAGCCTATGTTCACAACAATAGGCATCAGGTGATTGGCCTTTTTCTGGGTTTGTTTTTTCTTGTCGATCGAGGTTTTTGTATGTAGTCGATGTGTTGATTGATTGATTGATTGATTGATTGAAAGCCTCATTAGATGAACGAGTTCTTTTTTAATAATTTGGAACATCGAGGTTAGAAG

mRNA sequence

ATGAAATTGTTAAGCCCTAGGACGCCTTGTTCTTCCACCTTCCGTCTTCACAACCTCTTCAGTTTGCATTATCCCACAGCCTCCGCCGCCGCCGCAGCACACTTCTTCACGGACGTTACACCCTCTCTTGATCTCAGTGTGGTAACTCAGCACAATTCGATAATGCAGCCGACCGAAGCCTCCACTTCTATGGAAGTAGAAACGGTCTCAACCGAAACAAAGAATGTGGTTAGCACATTGCCACCGAAACCACAGTTTGATCCCCTAAAGCCACATGAGATGAATGATGGACGAATTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGCTATACGCCTCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAACAGATGAAAATCGACATCCGAATGAATCTCAAGGCGCGTAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGACATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTTCATTCTTGGGTTCGATGTTATCGATGCCATTGCCCTCCTAAGGGTGGATGAACTCTATGTTGAATCTTTTGAGATTAAGGATGTTAAAACGCTACGAGGCGAGCACTTGTCGCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACCAAATTTGCCATTGAAAACGCCACGAAGACGAGGATCGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTGATTTTAGGGTCCCCTGCAGGTAAAGTTTATTCAAAACTAAGGGCGGTTACAGCCAGATTAGCAGAAAGGTTTTGATCTTTCATGATCATAATGAGATTCATGCTGAGTTTGTCTTGATTAATGAATTTTGCATACACTAGTTGTCTGAGGAACAGAGTGTATCTTTGTCGAAAAGATTGCACATTGAAGAAGTTGTCTCATTAAGTAGCCTATGTTCACAACAATAGGCATCAGGTGATTGGCCTTTTTCTGGGTTTGTTTTTTCTTGTCGATCGAGGTTTTTGTATGTAGTCGATGTGTTGATTGATTGATTGATTGATTGATTGAAAGCCTCATTAGATGAACGAGTTCTTTTTTAATAATTTGGAACATCGAGGTTAGAAG

Coding sequence (CDS)

ATGAAATTGTTAAGCCCTAGGACGCCTTGTTCTTCCACCTTCCGTCTTCACAACCTCTTCAGTTTGCATTATCCCACAGCCTCCGCCGCCGCCGCAGCACACTTCTTCACGGACGTTACACCCTCTCTTGATCTCAGTGTGGTAACTCAGCACAATTCGATAATGCAGCCGACCGAAGCCTCCACTTCTATGGAAGTAGAAACGGTCTCAACCGAAACAAAGAATGTGGTTAGCACATTGCCACCGAAACCACAGTTTGATCCCCTAAAGCCACATGAGATGAATGATGGACGAATTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGCTATACGCCTCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAACAGATGAAAATCGACATCCGAATGAATCTCAAGGCGCGTAAAGTCGAGTTGAAAACCAGAGCAGACACACCTGACATCAGTAACTTGCAGAAGTGTGCAGATTTTGTTCATGCTTTCATTCTTGGGTTCGATGTTATCGATGCCATTGCCCTCCTAAGGGTGGATGAACTCTATGTTGAATCTTTTGAGATTAAGGATGTTAAAACGCTACGAGGCGAGCACTTGTCGCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACCAAATTTGCCATTGAAAACGCCACGAAGACGAGGATCGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTTGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTGATTTTAGGGTCCCCTGCAGGTAAAGTTTATTCAAAACTAAGGGCGGTTACAGCCAGATTAGCAGAAAGGTTTTGA

Protein sequence

MKLLSPRTPCSSTFRLHNLFSLHYPTASAAAAAHFFTDVTPSLDLSVVTQHNSIMQPTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFILGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAERF

Homology

[Close]

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot
Match: A7RP64 (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 4.9e-66
Identity = 121/196 (61.73%), Postives = 157/196 (80.10%), Query Frame = 0


Query: 83  KPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 142
           +P F P+K  ++  G+ + RK+ VP HRYTPLK+ WM+I+TP+ E +K+ IR NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 143 ELKTRADTPDISNLQKCADFVHAFILGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 202
           E++   +T DI  +QK ADFV AFILGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 203 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 262
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 263 VYSKLRAVTARLAERF 279
           VY  +RAV +R AERF
Sbjct: 223 VYGNMRAVASRSAERF 238

[Close]

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot
Match: Q9VR89 (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 8.4e-66
Identity = 122/187 (65.24%), Postives = 156/187 (83.42%), Query Frame = 0


Query: 91  PHEMNDGRIQFRKVSVPPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADT 150
           P +    R + RKVSVPPHRY+ LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 151 PDISNLQKCADFVHAFILGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 210
           PDI+NLQ+ ADFV AF+ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 211 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 270
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 271 TARLAER 278
            +RL+ER
Sbjct: 233 ASRLSER 239

[Close]

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot
Match: Q6VEU3 (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 248.8 bits (634), Expect = 7.1e-65
Identity = 124/211 (58.77%), Postives = 161/211 (76.30%), Query Frame = 0


Query: 69  VSTETKNVVSTLPPK-PQFDPLKPHEMNDGRIQFRKVSVPPHRYTPLKKAWMEIYTPIYE 128
           + T+T+   S+ P K P F  L   ++  G  + RKV VP HRYTPLK+ W++I+TPI E
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 129 QMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFILGFDVIDAIALLRVDELYVE 188
            +++ +R NLK R+VE+KT  +T DI  L + ADFV AF+LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 189 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 248
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 249 RDSLCSLILGSPAGKVYSKLRAVTARLAERF 279
           R ++C+LILGSP  KVY  LRAV  R AERF
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAERF 252

[Close]

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot
Match: Q5F414 (RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-64
Identity = 121/196 (61.73%), Postives = 156/196 (79.59%), Query Frame = 0


Query: 83  KPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKARKV 142
           +P F PL    +  G+ + RKV VP +RYTPLK+ WM+I+TPI E +++ IR NLK R V
Sbjct: 47  RPAFPPLPAAALGVGKGEVRKVPVPANRYTPLKENWMKIFTPIVEHLQLQIRFNLKTRNV 106

Query: 143 ELKTRADTPDISNLQKCADFVHAFILGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 202
           E+KT ++T D+S L K ADFV AFILGF V DA+AL+R+D+L++ESFE+ DVK L+G+HL
Sbjct: 107 EIKTCSETKDLSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEVTDVKPLKGDHL 166

Query: 203 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 262
           SRAIGR++GK GKTKF IEN T+TRIV+AD+KIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 167 SRAIGRIAGKGGKTKFTIENVTRTRIVLADSKIHILGSFQNIKMARTAICNLILGSPPSK 226

Query: 263 VYSKLRAVTARLAERF 279
           VY  +RAV +R AERF
Sbjct: 227 VYGNIRAVASRAAERF 242

[Close]

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot
Match: Q29IG6 (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.2e-64
Identity = 119/178 (66.85%), Postives = 153/178 (85.96%), Query Frame = 0


Query: 100 QFRKVSVPPHRYTPLKKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKC 159
           + RKVSVPPHRY+ LK+ WM+I+TP+ E MK+ IR N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 160 ADFVHAFILGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 219
           ADFV AF+ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 220 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAER 278
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+ER
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSER 237

[Close]

BLAST of CmaCh16G009770 vs. TAIR 10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein )

HSP 1 Score: 345.1 bits (884), Expect = 5.2e-95
Identity = 173/216 (80.09%), Postives = 198/216 (91.67%), Query Frame = 0


Query: 61  STSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPLKKAWME 120
           ST MEVET +  T      LPPKP F PLK HEM+DG++QFRK++VPP+RY+PLKKAW++
Sbjct: 4   STQMEVETATEGT----VPLPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 121 IYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFILGFDVIDAIALLR 180
           IYTPIY+QMK+DIRMNLKARKVELKTRADTPDISNLQK ADFVHAF+LGFD+ DAI+LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 181 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 240
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 241 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 277
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

The following BLAST results are available for this feature:

BLAST of CmaCh16G009770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7RP64	4.9e-66	61.73	RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1	[more]
Q9VR89	8.4e-66	65.24	RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV...	[more]
Q6VEU3	7.1e-65	58.77	RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2	[more]
Q5F414	1.2e-64	61.73	RNA-binding protein PNO1 OS=Gallus gallus OX=9031 GN=PNO1 PE=2 SV=1	[more]
Q29IG6	1.2e-64	66.85	RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l...	[more]

BLAST of CmaCh16G009770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13230.1	5.2e-95	80.09	RNA-binding KH domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004087	K Homology domain	SMART	SM00322	kh_6	coord: 183..256 e-value: 1.7E-4 score: 31.0
IPR036612	K Homology domain, type 1 superfamily	GENE3D	3.30.1370.10	K Homology domain, type 1	coord: 182..278 e-value: 1.8E-22 score: 81.3
IPR036612	K Homology domain, type 1 superfamily	SUPERFAMILY	54791	Eukaryotic type KH-domain (KH-domain type I)	coord: 183..277
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 73..92
None	No IPR available	PANTHER	PTHR12826	RIBONUCLEASE Y	coord: 61..278
None	No IPR available	CDD	cd00105	KH-I	coord: 203..249 e-value: 0.00552809 score: 32.9172
IPR039912	Pre-rRNA-processing protein PNO1-like	PANTHER	PTHR12826:SF13	RNA-BINDING PROTEIN PNO1	coord: 61..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009770.1	CmaCh16G009770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003676	nucleic acid binding