CmaCh16G009590 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G009590
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionPoly(A)-specific ribonuclease PARN
LocationCma_Chr16: 7431718 .. 7435340 (+)
RNA-Seq ExpressionCmaCh16G009590
SyntenyCmaCh16G009590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAGACTCAGCGATGAATCTGTCTCGCCCTCTTTGGGCTCCCACGCGGCTGCTTTGCTTCATCAGAACTGCAACGTACTCCTCCGCCGCTGTTACTGCTCCGGCCACCGCCTCAAACTCTAGCTTTCCCCTCAAGAATGTGACCAAGTCGAACTTTGAAGCTGTGCTATCGGAACTTCGGAGCCATGTCCGAGCTGCGGACTTCGTTGCCATCGACCTTGAAATGACCGGCATCACTAGCGCGCCTTGGCGCGAATCGTTCGAGTTTGATCGCTTTGATGTTCGCTACCTCAAGGTCAAGGACTCTGCTGAGAAGTTTGCCGTTGTTCAATTCGGCGTCTGCCCGTTCCGTTGGGACTCTGCCAAGAACTCATTCGTCGCTCATCCGTAATTTCTTTTCTTCTTCGTTGGATTTGGATTTCATTTGGTTACTGCGAAATTTCGTGTGAACCAGGCAGTATAGCCAGCTTACCGTGATGGAGAGATGGTTTCTTTTGTGGAATTTGACATTGTCTCGTTGTTTGAGTTGCAGGCATAACTTTTTTGTTTTCCCGCGCCATGAAATCCAAGTCGATGGCCCTTCTTCCGAGTTCCTCTGTCAGACTACCTCCATCGAATTCTTGGCTAAATACCAGTTTGATTTCAACGCTTGTATACATGAAGGTAGTTTTGTTTTCATGTTTCTCCTTCAATTTATGGACATAAAATTTATCTTCAGGATACAGAGACTGCGTCGAATTCATTTTATACGGTTATTCAGTCAGATTTGACAAGCATAATTGTAAACTTTTAGTCATTTCATTGATTGACTCTTTTGGTCCTCTCGGATTTTGAGAATTTGAATTGTAATTAATCTCAGGAATATCTTATTTGTCCAGAGCACATGAAGATGCGGCACGCAAACGCCTGAATTTTGCACATGGGGACGAAACGTTAGATGCATCTTCTAATGTGACTAATATTCGGAATCAGAAGAGGATCGGCATTGCTGATGTTTTATTCAAGGAAAGAATGAAAAACAGATTACATGAATGGCGGGACAGATTACTATCTGAAAGAAACATGGAGTGTCAAGGCCAGGAAGGCGCAAATGATTACCAACGACAATTTGAAACCATTTTCTTCAAGATGCGCCCCGCTCTTACTTTGAGCGATTTCACCTCTCATCAGACAAAATTGATTCAGCTGGTGAGATAGATTTTCATTTGCATATTTTACATCAATTTTAAACTTAAATGCACAAGTTTGTTTGCTTGCTTTATACGGAGCATGAAACATAATGGGAATTAGAGTACAGGGAAATATGATTTTATTATTCTAAGAAGATGCCATTTTCTGAAGTTGCTAGAGGCTTTGTTCTTGTTTCTTTTGTTTGTTCTGTGAAGAAACTATCTGTGGCATAAAATAATTTTCTGATGCAATATTTGCCTACTTTCTCATCAAGTATGTCTATGCTTGGCTGCGTGTTAGTTAACTTAACTGTCTGAATATTAATCATGCAATGTCGTTTGTTTGGGATAACATGATCTTATTTCATTTGAAATGTTGATCATGTGGAGGTGAACACATGCAGGTCATTAAGAAGCATTTTGAAGATCTTTGTTTCGTTGTCGTGGATGATGGCAATGCCAATCTACAACAATTAGTCGTGTACGTGGATTCTAGGGACGATAAAGAGCTGCTCATGGTTAGACACTTTCCTTTTGTGCTCACAAAACCTTGACTACAAACTAGCAACTGAAAAAGTCGAAATGAAGACAAATATCTTGACTTGGTTACTTTTATTTCGAACTTTTCAATTTACGTTTAAATCTGTGCTTATTAGATGTTTTAGTTCGTAAAACTAAGCGCTGAAAGTGGTAATCTTTCTGAAATTCGTTGTGAAAATCTATATGTTGTTGTGTATTGGATCAGCAGTAATGGAAAACAGTACATGAGGCCTTATTCTTGCTTATCACTTCATGCTTTTGGGAGAGAGATATGGAAGTAAAAATGCATTTACAAAGTTACAGCCCCGAATTGCTTATAATGAATTGTCATATTAATGCAGCAAGAGGTCAACTGCGATCGGCAGGAAGCAGCTGAAATGAAGATCCAATCAGCAATTGGGTTTCGTCATGTTATCGATCTCCTTTCATCGGAAAAGAAGTTGATTATCGGTCATAATTGCTTCCTCGGTAAGGCATTCAGCCTTAGTTTCATAGACAATAATTGTTAACAATTCTATTTTCAGCTGACACAACTGTTTTCTGTACCAGATATTGCCCACATACACAAAAAGTTTATAGGTTCTCTTCCTTTAACGGCTGAAGAATTTGTCTCTTCTATTGGAAAGCGCTTTCCATTAATCATGGACACCAAAATTCTATTAAACGGAAATGGCATTCTTCAGCAACGGATGAAGAAGTCCAGCACATCTCTATCCTCTGCATTTGCCTTATTATGCCCGCATATTGCCTTTAGCTCTCAGAGCAATCCTGCCTTCAACTCTGGTGTGCAAGTGGAGGTTCAAGTAGACGATCTAAGGTACGTTTTCCTCGGCATATTTGGACGGCTAGGATTTTCTATATCGTATGCTGAATATAAGTTTCTATTCGTTATCCAAATTTTCTATATCGAGTGCTACCAATAGAAAACTTATGGGTGAAATTGTTGAATTTCTCATCATTTTTGTATAAATACAAGTGCACGTGTGTGTCGATTTAGCATTGATGTTTTGATCACTAGTGTTGTATTCTTGATCCTGGCAGTACTTAGGATTTGAAGGTACTTAATTGATTAAAATTAAGTAAAATCTAACTGCATCTATGTTTTAATTTCAACCTCTATGCAGTTCCTCCAACTGGAATTCTGGAGATAAGCATGAAGCTGGGTACGATGCTTTTATGACAGGATGTGTATTTGCACAAGCGTGCAATCTTATGGGAATTGATTTTGTTCTTCCTTCTGAAAATTTGGCTGACAATGAGAAATTGCAGAAGCACATAAACCATCTGTATTTGAGTTGGGCCAGTGGCGATATTATCGATCTGAGCACCGGTAATCGAATGACCGAGCCTTTGAGGTCTAATAGATCTAAAAGGCATCAAGTAAAAATATCGTTCGAGAACATTGTTCTAATATGGGGATTTCCTGCCAACCTTAATGCAAGGAAAATCAAAGAGTGCATCTCTAAAGTGTTCGGCCTGGCAAATATCGTTAGTGTCTACCATATAGATGAAAGTGCTGTGTTTGTGCAGTTTGCAAAGCCAGAAATGGTTTCCGAATTTCTCGACCTAAAGGAAAACTTGGAGCGGCACGATGATCCAATATCAGTTTTGCATCCTCTCACTGAGCTTTTTGGAGCTAAAAGTACTCACGCTGCAGGCTATGAAGCATACAAGGAAATTTGCAGCTCTCCCATGTTTAAAGTCTTGTTTGCTGATCAGGCAGAGGCAGTTGGGATTAATCAGAAGACGATGTCGATAAAACTCAAGTCAGTAAAGGGCCAGATGCATGAAGCTGCCAACAGCAGTTTAACTGCTGATATGTTGAAGGAAACTACTGAAGTACAGGGGATGGAAGTGGGAGCAAATGATCTTAGAAACACTAACTACCATATTTTACGATCTTCTTCGATGTAA

mRNA sequence

GTAAGACTCAGCGATGAATCTGTCTCGCCCTCTTTGGGCTCCCACGCGGCTGCTTTGCTTCATCAGAACTGCAACGTACTCCTCCGCCGCTGTTACTGCTCCGGCCACCGCCTCAAACTCTAGCTTTCCCCTCAAGAATGTGACCAAGTCGAACTTTGAAGCTGTGCTATCGGAACTTCGGAGCCATGTCCGAGCTGCGGACTTCGTTGCCATCGACCTTGAAATGACCGGCATCACTAGCGCGCCTTGGCGCGAATCGTTCGAGTTTGATCGCTTTGATGTTCGCTACCTCAAGGTCAAGGACTCTGCTGAGAAGTTTGCCGTTGTTCAATTCGGCGTCTGCCCGTTCCGTTGGGACTCTGCCAAGAACTCATTCGTCGCTCATCCGCATAACTTTTTTGTTTTCCCGCGCCATGAAATCCAAGTCGATGGCCCTTCTTCCGAGTTCCTCTGTCAGACTACCTCCATCGAATTCTTGGCTAAATACCAGTTTGATTTCAACGCTTGTATACATGAAGGAATATCTTATTTGTCCAGAGCACATGAAGATGCGGCACGCAAACGCCTGAATTTTGCACATGGGGACGAAACGTTAGATGCATCTTCTAATGTGACTAATATTCGGAATCAGAAGAGGATCGGCATTGCTGATGTTTTATTCAAGGAAAGAATGAAAAACAGATTACATGAATGGCGGGACAGATTACTATCTGAAAGAAACATGGAGTGTCAAGGCCAGGAAGGCGCAAATGATTACCAACGACAATTTGAAACCATTTTCTTCAAGATGCGCCCCGCTCTTACTTTGAGCGATTTCACCTCTCATCAGACAAAATTGATTCAGCTGGTCATTAAGAAGCATTTTGAAGATCTTTGTTTCGTTGTCGTGGATGATGGCAATGCCAATCTACAACAATTAGTCGTGTACGTGGATTCTAGGGACGATAAAGAGCTGCTCATGCAAGAGGTCAACTGCGATCGGCAGGAAGCAGCTGAAATGAAGATCCAATCAGCAATTGGGTTTCGTCATGTTATCGATCTCCTTTCATCGGAAAAGAAGTTGATTATCGGTCATAATTGCTTCCTCGATATTGCCCACATACACAAAAAGTTTATAGGTTCTCTTCCTTTAACGGCTGAAGAATTTGTCTCTTCTATTGGAAAGCGCTTTCCATTAATCATGGACACCAAAATTCTATTAAACGGAAATGGCATTCTTCAGCAACGGATGAAGAAGTCCAGCACATCTCTATCCTCTGCATTTGCCTTATTATGCCCGCATATTGCCTTTAGCTCTCAGAGCAATCCTGCCTTCAACTCTGGTGTGCAAGTGGAGGTTCAAGTAGACGATCTAAGTTCCTCCAACTGGAATTCTGGAGATAAGCATGAAGCTGGGTACGATGCTTTTATGACAGGATGTGTATTTGCACAAGCGTGCAATCTTATGGGAATTGATTTTGTTCTTCCTTCTGAAAATTTGGCTGACAATGAGAAATTGCAGAAGCACATAAACCATCTGTATTTGAGTTGGGCCAGTGGCGATATTATCGATCTGAGCACCGGTAATCGAATGACCGAGCCTTTGAGGTCTAATAGATCTAAAAGGCATCAAGTAAAAATATCGTTCGAGAACATTGTTCTAATATGGGGATTTCCTGCCAACCTTAATGCAAGGAAAATCAAAGAGTGCATCTCTAAAGTGTTCGGCCTGGCAAATATCGTTAGTGTCTACCATATAGATGAAAGTGCTGTGTTTGTGCAGTTTGCAAAGCCAGAAATGGTTTCCGAATTTCTCGACCTAAAGGAAAACTTGGAGCGGCACGATGATCCAATATCAGTTTTGCATCCTCTCACTGAGCTTTTTGGAGCTAAAAGTACTCACGCTGCAGGCTATGAAGCATACAAGGAAATTTGCAGCTCTCCCATGTTTAAAGTCTTGTTTGCTGATCAGGCAGAGGCAGTTGGGATTAATCAGAAGACGATGTCGATAAAACTCAAGTCAGTAAAGGGCCAGATGCATGAAGCTGCCAACAGCAGTTTAACTGCTGATATGTTGAAGGAAACTACTGAAGTACAGGGGATGGAAGTGGGAGCAAATGATCTTAGAAACACTAACTACCATATTTTACGATCTTCTTCGATGTAA

Coding sequence (CDS)

ATGAATCTGTCTCGCCCTCTTTGGGCTCCCACGCGGCTGCTTTGCTTCATCAGAACTGCAACGTACTCCTCCGCCGCTGTTACTGCTCCGGCCACCGCCTCAAACTCTAGCTTTCCCCTCAAGAATGTGACCAAGTCGAACTTTGAAGCTGTGCTATCGGAACTTCGGAGCCATGTCCGAGCTGCGGACTTCGTTGCCATCGACCTTGAAATGACCGGCATCACTAGCGCGCCTTGGCGCGAATCGTTCGAGTTTGATCGCTTTGATGTTCGCTACCTCAAGGTCAAGGACTCTGCTGAGAAGTTTGCCGTTGTTCAATTCGGCGTCTGCCCGTTCCGTTGGGACTCTGCCAAGAACTCATTCGTCGCTCATCCGCATAACTTTTTTGTTTTCCCGCGCCATGAAATCCAAGTCGATGGCCCTTCTTCCGAGTTCCTCTGTCAGACTACCTCCATCGAATTCTTGGCTAAATACCAGTTTGATTTCAACGCTTGTATACATGAAGGAATATCTTATTTGTCCAGAGCACATGAAGATGCGGCACGCAAACGCCTGAATTTTGCACATGGGGACGAAACGTTAGATGCATCTTCTAATGTGACTAATATTCGGAATCAGAAGAGGATCGGCATTGCTGATGTTTTATTCAAGGAAAGAATGAAAAACAGATTACATGAATGGCGGGACAGATTACTATCTGAAAGAAACATGGAGTGTCAAGGCCAGGAAGGCGCAAATGATTACCAACGACAATTTGAAACCATTTTCTTCAAGATGCGCCCCGCTCTTACTTTGAGCGATTTCACCTCTCATCAGACAAAATTGATTCAGCTGGTCATTAAGAAGCATTTTGAAGATCTTTGTTTCGTTGTCGTGGATGATGGCAATGCCAATCTACAACAATTAGTCGTGTACGTGGATTCTAGGGACGATAAAGAGCTGCTCATGCAAGAGGTCAACTGCGATCGGCAGGAAGCAGCTGAAATGAAGATCCAATCAGCAATTGGGTTTCGTCATGTTATCGATCTCCTTTCATCGGAAAAGAAGTTGATTATCGGTCATAATTGCTTCCTCGATATTGCCCACATACACAAAAAGTTTATAGGTTCTCTTCCTTTAACGGCTGAAGAATTTGTCTCTTCTATTGGAAAGCGCTTTCCATTAATCATGGACACCAAAATTCTATTAAACGGAAATGGCATTCTTCAGCAACGGATGAAGAAGTCCAGCACATCTCTATCCTCTGCATTTGCCTTATTATGCCCGCATATTGCCTTTAGCTCTCAGAGCAATCCTGCCTTCAACTCTGGTGTGCAAGTGGAGGTTCAAGTAGACGATCTAAGTTCCTCCAACTGGAATTCTGGAGATAAGCATGAAGCTGGGTACGATGCTTTTATGACAGGATGTGTATTTGCACAAGCGTGCAATCTTATGGGAATTGATTTTGTTCTTCCTTCTGAAAATTTGGCTGACAATGAGAAATTGCAGAAGCACATAAACCATCTGTATTTGAGTTGGGCCAGTGGCGATATTATCGATCTGAGCACCGGTAATCGAATGACCGAGCCTTTGAGGTCTAATAGATCTAAAAGGCATCAAGTAAAAATATCGTTCGAGAACATTGTTCTAATATGGGGATTTCCTGCCAACCTTAATGCAAGGAAAATCAAAGAGTGCATCTCTAAAGTGTTCGGCCTGGCAAATATCGTTAGTGTCTACCATATAGATGAAAGTGCTGTGTTTGTGCAGTTTGCAAAGCCAGAAATGGTTTCCGAATTTCTCGACCTAAAGGAAAACTTGGAGCGGCACGATGATCCAATATCAGTTTTGCATCCTCTCACTGAGCTTTTTGGAGCTAAAAGTACTCACGCTGCAGGCTATGAAGCATACAAGGAAATTTGCAGCTCTCCCATGTTTAAAGTCTTGTTTGCTGATCAGGCAGAGGCAGTTGGGATTAATCAGAAGACGATGTCGATAAAACTCAAGTCAGTAAAGGGCCAGATGCATGAAGCTGCCAACAGCAGTTTAACTGCTGATATGTTGAAGGAAACTACTGAAGTACAGGGGATGGAAGTGGGAGCAAATGATCTTAGAAACACTAACTACCATATTTTACGATCTTCTTCGATGTAA

Protein sequence

MNLSRPLWAPTRLLCFIRTATYSSAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIFFKMRPALTLSDFTSHQTKLIQLVIKKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAAEMKIQSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEEFVSSIGKRFPLIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFNSGVQVEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPSENLADNEKLQKHINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIKECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTELFGAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKTMSIKLKSVKGQMHEAANSSLTADMLKETTEVQGMEVGANDLRNTNYHILRSSSM
Homology
BLAST of CmaCh16G009590 vs. ExPASy Swiss-Prot
Match: Q9LG26 (Poly(A)-specific ribonuclease PARN OS=Arabidopsis thaliana OX=3702 GN=PARN PE=1 SV=2)

HSP 1 Score: 750.0 bits (1935), Expect = 2.5e-215
Identity = 378/650 (58.15%), Postives = 477/650 (73.38%), Query Frame = 0

Query: 10  PTRLLCFIRTATYSSAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVRAADFVAIDL 69
           P R L    +++ +    T+ A ++ ++FPLK+VT+SNFE  L++LRS V+AADFVAIDL
Sbjct: 8   PLRSLVCSFSSSAAETVTTSTAASATAAFPLKHVTRSNFETTLNDLRSLVKAADFVAIDL 67

Query: 70  EMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFF 129
           EMTG+TSAPWR+S EFDR+DVRYLKVKDSAEKFAVVQFGVCPFRWDS   SFV++PHNFF
Sbjct: 68  EMTGVTSAPWRDSLEFDRYDVRYLKVKDSAEKFAVVQFGVCPFRWDSRTQSFVSYPHNFF 127

Query: 130 VFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAH 189
           VFPR E+  D P+ EFLCQTTS++FLAKYQFDFN CIHEGISYLSR  E+ A KRL   H
Sbjct: 128 VFPRQELTFDPPAHEFLCQTTSMDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLH 187

Query: 190 GDETLDASSNVTNIRNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQ 249
           G++ +D+S     +   K + +ADVLF  RM+  L+EWR  LL   N   +    +N   
Sbjct: 188 GEDGIDSSGETEEL---KLVRLADVLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSN 247

Query: 250 RQFETIFFKMRPALTLSDFTSHQTKLIQLVIKKHFEDLCFVVVDDGNANLQQLVVYVDSR 309
           +  ET+F  MRPAL+L  FTSHQ +++  V++KHF DL ++  +D +++ + +VVY DS 
Sbjct: 248 QSMETVFHHMRPALSLKGFTSHQLRVLNSVLRKHFGDLVYIHSNDKSSSSRDIVVYTDSD 307

Query: 310 DDKELLMQEVNCDRQEAAEMKIQSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIG 369
            DKE LM+E   +R+  AE KIQSAIGFR VIDLL+SEKKLI+GHNCFLDIAH++ KF+G
Sbjct: 308 SDKENLMKEAKDERKRLAERKIQSAIGFRQVIDLLASEKKLIVGHNCFLDIAHVYSKFVG 367

Query: 370 SLPLTAEEFVSSIGKRFPLIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQ 429
            LP TAE+FV+SI   FP I+DTKILLN N +L QRMKKSSTSLSSAF+ LCP I FSS+
Sbjct: 368 PLPSTAEKFVASINSHFPYIVDTKILLNVNPMLHQRMKKSSTSLSSAFSSLCPQIEFSSR 427

Query: 430 SNPAF-NSGVQVEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPS-- 489
           S+ +F    V ++V++D++  SNWN+G KHEAGYDAFMTGC+FAQACN +G DF   S  
Sbjct: 428 SSDSFLQQRVNIDVEIDNVRCSNWNAGGKHEAGYDAFMTGCIFAQACNHLGFDFKQHSQL 487

Query: 490 ENLADNEKLQKHINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWG 549
           ++ A NEKL+K+IN LYLSW  GDIIDL TG+       SN       K  +ENIVLIW 
Sbjct: 488 DDFAQNEKLEKYINRLYLSWTRGDIIDLRTGH-------SNADNWRVSKFKYENIVLIWN 547

Query: 550 FPANLNARKIKECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDP 609
           FP  L AR IKECI K FG A++ SVYH+D+SAVFV F   E+V +FL LK  LE  D P
Sbjct: 548 FPRKLKARGIKECICKAFGSASVTSVYHVDDSAVFVLFKNSELVWDFLALKRQLESSDGP 607

Query: 610 ISVLHPLTELFGAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKT 657
           +SVLHPL+++    +T AA YEAYKEICSS + +V+F+DQAE VG+  +T
Sbjct: 608 VSVLHPLSKILEGGNTGAADYEAYKEICSSHVSEVMFSDQAETVGVKSRT 647

BLAST of CmaCh16G009590 vs. ExPASy Swiss-Prot
Match: Q8W4C3 (Poly(A)-specific ribonuclease PARN-like OS=Arabidopsis thaliana OX=3702 GN=At3g25430 PE=2 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.3e-57
Identity = 176/639 (27.54%), Postives = 297/639 (46.48%), Query Frame = 0

Query: 24  SAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESF 83
           SA      T +   + +K V KSNF   L E+R+ + ++DF+A+ L+ TG  +A W    
Sbjct: 10  SATAGNTKTLNQGRWSVKQVKKSNFHVTLDEIRTSIDSSDFIALSLQNTGSYAAAWHRVS 69

Query: 84  EFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSS 143
             D     YLK K +AE++ ++QF +CPF    +K     HP+NF +FPR E++   PS 
Sbjct: 70  AIDTPQTSYLKAKYAAERYQILQFALCPFSLQGSK--LTVHPYNFHLFPRDELKCGMPSY 129

Query: 144 EFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNI 203
            F CQ + +  +A+  FDFN CI+EGISYLSRA E A++    F   +  L  S  V++ 
Sbjct: 130 SFSCQASRLTAMAREGFDFNICIYEGISYLSRAQESASK----FLSENPILADSVTVSS- 189

Query: 204 RNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIF-----FK 263
                  +AD +F  R+++R+  WR   +     +   + G +D       +      + 
Sbjct: 190 ---SPATVADTVFVGRIRSRVKNWRQSCI-----DSGSKTGDDDLVSSLRRLVLGSEQYG 249

Query: 264 MRPALTLSDFTSHQTKLIQLVIKKHFEDLCFVVVDDGNANLQQL-VVYVDSRDDKELLMQ 323
            R  LT+   +  Q +LI  ++ +  +D+  ++V   +   Q +  V++ S++DK+L  +
Sbjct: 250 SRLCLTIDVCSERQVQLILEMLTEFSDDVVPLLVASKSRGTQAVRTVFMSSKEDKDLFKR 309

Query: 324 EVNCDRQEAAEMKIQSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEE 383
           E+  D ++    +++   GFR V+D +SS +K ++  N   D   IH KF+G LP   ++
Sbjct: 310 ELK-DLEKEENRRVR---GFREVVDFISSSQKPVVSQNYLSDFTSIHAKFLGPLPSNVDD 369

Query: 384 FVSSIGKRFPLIMDTKILLNGNGILQ--QRMKKSSTSLSSAFALLCPHIAFSSQSNPAFN 443
           F SS+   FP ++D    +     L     +  + +SL+  FA +   +A         N
Sbjct: 370 FSSSLSSAFPNVVDLSQFMKEISPLSNISNLPAAMSSLNRFFAPVDVEVA---------N 429

Query: 444 SGVQVEVQVDDLSSSNWNSGDKHEA-GYDAFMTGCVFAQACNLMGIDFVLPSENLADNEK 503
            G  V++             + H++ G +A M   +FA+ C +   D       +  NE 
Sbjct: 430 QGCPVKLD------------EGHQSHGQNAVMISQLFAKLCTIQKSDL----STIQSNED 489

Query: 504 LQKHINHLYLSWASGDIIDLSTGNRMTEPLR--SNRSKRHQVKISFENIVLIWGFPANLN 563
            Q        S    + +   + N   E ++  S  S+R    +S EN+V IWG    + 
Sbjct: 490 FQ-----ALASDEHANSVTSCSKNAGDENVKVWSKNSRR----VSSENLVFIWGLGKKMT 549

Query: 564 ARKIKECISKVFGL-ANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLH 623
           A K+K  + K   + A    V +ID S+  + F +      FL    N E+ D  +  + 
Sbjct: 550 AAKLKNVLQKSHPVFAREFDVKYIDRSSAILVFWESGPSETFLSAVNNEEQLDGSLREM- 585

Query: 624 PLTELFGAKSTHAAGYEAYKEICSSPMFKVLFADQAEAV 651
                  A+    AGYE YK  C    ++   AD AE++
Sbjct: 610 ------VAEGLRGAGYETYKRACRLGFWE---ADLAESL 585

BLAST of CmaCh16G009590 vs. ExPASy Swiss-Prot
Match: Q90ZA1 (Poly(A)-specific ribonuclease PARN OS=Xenopus laevis OX=8355 GN=parn PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 5.3e-40
Identity = 147/560 (26.25%), Postives = 249/560 (44.46%), Query Frame = 0

Query: 43  VTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESFE--FDRFDVRYLKVKDSAE 102
           +T+SNF+  L ++   +  ADF+AID E +GI+  P   +    FD  + RY K+K  + 
Sbjct: 3   ITRSNFKDTLPKVYKAIEEADFLAIDGEFSGISDGPSVSTLTNGFDTPEERYTKLKKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQF 162
           +F + QFG+C F +D+ +  ++    NF++FP+     + P  +F+CQ++SI+FLA   F
Sbjct: 63  EFLLFQFGLCTFNYDNTEAKYLMKSFNFYIFPK-PFNRNSPDKKFVCQSSSIDFLANQGF 122

Query: 163 DFNACIHEGISYLSRAHE----DAARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVL- 222
           DFN     GI YL++  E    D    R + ++G  T+   S ++   ++  + I D   
Sbjct: 123 DFNKVFRNGIPYLNQEEERVLRDQYEDRRSQSNGASTM---SYISPNSSKTPVSIPDEQK 182

Query: 223 -FKERMKNRLHEWRDRLLSERNME-CQGQEGANDYQRQ--FETIFFKMRPALTLSDFTSH 282
            F +++  R+ ++        N+E C G      YQR+  ++T+ +K    + +    S 
Sbjct: 183 GFIDKVVERVEDFLKNEQKSMNVEPCTG------YQRKLIYQTLNWKYPRGIHVETVES- 242

Query: 283 QTKLIQLVIKKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAAEMKI 342
           + K   +VI K                       VD  + K +  Q+   +R+E     +
Sbjct: 243 EKKERYIVISK-----------------------VDEEERKRMEQQKQAKEREE-----L 302

Query: 343 QSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEEFVSSIGKRFPLIMD 402
             A+GF  +I  +SS  KL++GHN  LD+ H   +F   LP    EF       FP ++D
Sbjct: 303 DDAVGFSRIIQAISSSGKLVVGHNMLLDVMHTIHQFFCQLPDELNEFKEVTNCVFPRVLD 362

Query: 403 TKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFNSGVQVEVQVDDLSSSN 462
           TK++ + N              +++ A L   +  +    P  +S        +   S N
Sbjct: 363 TKLMASTNPF-------KEIIYNTSLAELEKRLKEAPFKPPKVDS-------AEGFQSYN 422

Query: 463 WNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPSENLADNEKL-QKHINHLYLSWASGD 522
             S   HEAGYDA++TG  F    N +G     P + ++   K+ +   N L+L     D
Sbjct: 423 TASEQLHEAGYDAYITGLCFISMANYLGSFLSPPKDYVSCRSKIVRPFFNKLFLMRIM-D 482

Query: 523 IIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIKECISKVFGLANIV 582
           I  L+      +P R N              VL   FP       + +    +F     +
Sbjct: 483 IPYLNLEGPDLQPKRDN--------------VLHVTFPKEWKTSDLYQ----LFSAFGNI 490

Query: 583 SVYHIDESAVFVQFAKPEMV 591
            V  ID+++ FV  ++PE V
Sbjct: 543 QVSWIDDTSAFVSLSQPEQV 490

BLAST of CmaCh16G009590 vs. ExPASy Swiss-Prot
Match: Q7ZU92 (Poly(A)-specific ribonuclease PARN OS=Danio rerio OX=7955 GN=parn PE=1 SV=2)

HSP 1 Score: 166.8 bits (421), Expect = 9.0e-40
Identity = 132/501 (26.35%), Postives = 222/501 (44.31%), Query Frame = 0

Query: 43  VTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESFE--FDRFDVRYLKVKDSAE 102
           VT+ NF+ VL E+ + V+ ADF++ID E TGI+  P   +     D  + RY K++  + 
Sbjct: 3   VTRQNFKEVLPEVCNAVQEADFISIDGEFTGISDGPSVSALTNGLDTPEERYTKLRKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQF 162
            F + QFGVC FR+D  +++++    NF++FP+       P  +F+CQ++SI+FLA   F
Sbjct: 63  NFLLFQFGVCTFRYDQNQSTYITKAFNFYIFPK-PFSRTSPDIKFICQSSSIDFLASQGF 122

Query: 163 DFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVLFKERM 222
           DFN     GI YL++  E   R++     G       +   +       G+ +V   +R 
Sbjct: 123 DFNKVFRSGIPYLNQEEECQLREQYEERRGQMN---GAGPVSYTPPSGTGVCNVPEDQR- 182

Query: 223 KNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIFFKMRPALTLSDFTSHQTKLIQLVI 282
                                 E     + + E +       L L   T  Q KLI   +
Sbjct: 183 ----------------------EFIRSVEEKVEALLKNTDQTLDLEPCTGFQRKLIYQTL 242

Query: 283 KKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAAEMKIQSAIGFRHV 342
              +     + V+      ++  + +   DD+E   +E    +Q+  + ++  A+GF  V
Sbjct: 243 NSKYSK--GLHVEALETEKKERFIQISKVDDEERRRREQ--QKQQREQEELNDAVGFSRV 302

Query: 343 IDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEEFVSSIGKRFPLIMDTKILLNGNG 402
           I  +S   KL++GHN  LD+ H   +F G LP   ++F       FP ++DTK++ +   
Sbjct: 303 IRAISKSGKLVVGHNMLLDVMHTIHQFCGPLPEELDDFKEVAMTVFPRLLDTKLMAS--- 362

Query: 403 ILQQRMKK--SSTSLSSAFALLCPHIAFSSQSNPAFNSGVQVEVQVDDLSSSNWNSGDKH 462
              Q  K+   +TSL+     L        +  P      +     + L S + ++   H
Sbjct: 363 --TQPFKEIIHNTSLAELHKQL--------RQKPFRPPTTECP---EGLQSYDTSTEQLH 422

Query: 463 EAGYDAFMTGCVFAQACNLMGIDFVLPSENLADNEK-LQKHINHLYLSWASGDIIDLSTG 522
           EAGYDAF+TG  F    N +G     P  +++   K L+   N L+L      +ID+   
Sbjct: 423 EAGYDAFITGLCFISMANYLGSFLTPPKSHISARSKLLEPFYNKLFLM----RVIDIPYL 450

Query: 523 NRMTEPLRSNRSKRHQVKISF 539
           N     L+  R   H + ++F
Sbjct: 483 NMSGPDLQPKRD--HVLYVTF 450

BLAST of CmaCh16G009590 vs. ExPASy Swiss-Prot
Match: P69341 (Poly(A)-specific ribonuclease PARN OS=Bos taurus OX=9913 GN=PARN PE=1 SV=2)

HSP 1 Score: 158.7 bits (400), Expect = 2.5e-37
Identity = 137/562 (24.38%), Postives = 241/562 (42.88%), Query Frame = 0

Query: 43  VTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESFE--FDRFDVRYLKVKDSAE 102
           + +SNF++ L ++   +  ADF AID E +GI+  P   +    FD  + RY K+K  + 
Sbjct: 3   IIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVTALTNGFDTPEERYQKLKKHSM 62

Query: 103 KFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSSEFLCQTTSIEFLAKYQF 162
            F + QFG+C F++D   + ++    NF+VFP+       P  +F+CQ++SI+FLA   F
Sbjct: 63  DFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPK-PFNRSSPDVKFVCQSSSIDFLASQGF 122

Query: 163 DFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNIRNQKRIGIADVLFKERM 222
           DFN     GI YL++  E   R++ +          + + T+    K      V   +  
Sbjct: 123 DFNKVFRNGIPYLNQEEERQLREQYDEKRSQSNGAGALSYTSPNTSK----CPVTIPDDQ 182

Query: 223 KNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIFFKMRPALTLSDFTSHQTKLIQLVI 282
           K    ++ D+++ +     Q +E  N                L L   T  Q KLI   +
Sbjct: 183 K----KFIDQVVEKIEDLLQSEENKN----------------LDLEPCTGFQRKLIYQTL 242

Query: 283 KKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAAEMKIQSAIGFRHV 342
              +     V   +     + +V+     ++++   Q+ +   QE    ++  A+GF  V
Sbjct: 243 SWKYPKGIHVETLETEKKERYIVISKVDEEERKRREQQKHAKEQE----ELNDAVGFSRV 302

Query: 343 IDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEEFVSSIGKRFPLIMDTKI------ 402
           I  +++  KL+IGHN  LD+ H   +F   LP    EF       FP ++DTK+      
Sbjct: 303 IHAIANSGKLVIGHNMLLDVMHTVHQFYCPLPADLNEFKEMTTCVFPRLLDTKLMASTQP 362

Query: 403 ---LLNGNGI--LQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAFNSGVQVEVQVDDLSS 462
              ++N   +  L++R+K++  +         P    S++  P++++             
Sbjct: 363 FKDIINNTSLAELEKRLKETPFN---------PPKVESAEGFPSYDTA------------ 422

Query: 463 SNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPSENLADNEKL-QKHINHLYLSWAS 522
               S   HEAGYDA++TG  F    N +G     P  +++   KL +   N L+L    
Sbjct: 423 ----SEQLHEAGYDAYITGLCFISMANYLGSFLSPPKSHVSARSKLIEPFFNKLFLM--- 482

Query: 523 GDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIKECISKVFGLAN 582
             ++D+   N     L+  R             VL   FP       + +    +F    
Sbjct: 483 -RVMDIPYLNLEGPDLQPKRDH-----------VLHVTFPKEWKTSDLYQ----LFSAFG 491

Query: 583 IVSVYHIDESAVFVQFAKPEMV 591
            + +  ID+++ FV  ++PE V
Sbjct: 543 NIQISWIDDTSAFVSLSQPEQV 491

BLAST of CmaCh16G009590 vs. TAIR 10
Match: AT1G55870.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 750.0 bits (1935), Expect = 1.8e-216
Identity = 378/650 (58.15%), Postives = 477/650 (73.38%), Query Frame = 0

Query: 10  PTRLLCFIRTATYSSAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVRAADFVAIDL 69
           P R L    +++ +    T+ A ++ ++FPLK+VT+SNFE  L++LRS V+AADFVAIDL
Sbjct: 8   PLRSLVCSFSSSAAETVTTSTAASATAAFPLKHVTRSNFETTLNDLRSLVKAADFVAIDL 67

Query: 70  EMTGITSAPWRESFEFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFF 129
           EMTG+TSAPWR+S EFDR+DVRYLKVKDSAEKFAVVQFGVCPFRWDS   SFV++PHNFF
Sbjct: 68  EMTGVTSAPWRDSLEFDRYDVRYLKVKDSAEKFAVVQFGVCPFRWDSRTQSFVSYPHNFF 127

Query: 130 VFPRHEIQVDGPSSEFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAH 189
           VFPR E+  D P+ EFLCQTTS++FLAKYQFDFN CIHEGISYLSR  E+ A KRL   H
Sbjct: 128 VFPRQELTFDPPAHEFLCQTTSMDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLH 187

Query: 190 GDETLDASSNVTNIRNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQ 249
           G++ +D+S     +   K + +ADVLF  RM+  L+EWR  LL   N   +    +N   
Sbjct: 188 GEDGIDSSGETEEL---KLVRLADVLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSN 247

Query: 250 RQFETIFFKMRPALTLSDFTSHQTKLIQLVIKKHFEDLCFVVVDDGNANLQQLVVYVDSR 309
           +  ET+F  MRPAL+L  FTSHQ +++  V++KHF DL ++  +D +++ + +VVY DS 
Sbjct: 248 QSMETVFHHMRPALSLKGFTSHQLRVLNSVLRKHFGDLVYIHSNDKSSSSRDIVVYTDSD 307

Query: 310 DDKELLMQEVNCDRQEAAEMKIQSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIG 369
            DKE LM+E   +R+  AE KIQSAIGFR VIDLL+SEKKLI+GHNCFLDIAH++ KF+G
Sbjct: 308 SDKENLMKEAKDERKRLAERKIQSAIGFRQVIDLLASEKKLIVGHNCFLDIAHVYSKFVG 367

Query: 370 SLPLTAEEFVSSIGKRFPLIMDTKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQ 429
            LP TAE+FV+SI   FP I+DTKILLN N +L QRMKKSSTSLSSAF+ LCP I FSS+
Sbjct: 368 PLPSTAEKFVASINSHFPYIVDTKILLNVNPMLHQRMKKSSTSLSSAFSSLCPQIEFSSR 427

Query: 430 SNPAF-NSGVQVEVQVDDLSSSNWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPS-- 489
           S+ +F    V ++V++D++  SNWN+G KHEAGYDAFMTGC+FAQACN +G DF   S  
Sbjct: 428 SSDSFLQQRVNIDVEIDNVRCSNWNAGGKHEAGYDAFMTGCIFAQACNHLGFDFKQHSQL 487

Query: 490 ENLADNEKLQKHINHLYLSWASGDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWG 549
           ++ A NEKL+K+IN LYLSW  GDIIDL TG+       SN       K  +ENIVLIW 
Sbjct: 488 DDFAQNEKLEKYINRLYLSWTRGDIIDLRTGH-------SNADNWRVSKFKYENIVLIWN 547

Query: 550 FPANLNARKIKECISKVFGLANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDP 609
           FP  L AR IKECI K FG A++ SVYH+D+SAVFV F   E+V +FL LK  LE  D P
Sbjct: 548 FPRKLKARGIKECICKAFGSASVTSVYHVDDSAVFVLFKNSELVWDFLALKRQLESSDGP 607

Query: 610 ISVLHPLTELFGAKSTHAAGYEAYKEICSSPMFKVLFADQAEAVGINQKT 657
           +SVLHPL+++    +T AA YEAYKEICSS + +V+F+DQAE VG+  +T
Sbjct: 608 VSVLHPLSKILEGGNTGAADYEAYKEICSSHVSEVMFSDQAETVGVKSRT 647

BLAST of CmaCh16G009590 vs. TAIR 10
Match: AT1G55870.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 553.9 bits (1426), Expect = 1.9e-157
Identity = 283/508 (55.71%), Postives = 361/508 (71.06%), Query Frame = 0

Query: 152 IEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNIRNQKRIGI 211
           ++FLAKYQFDFN CIHEGISYLSR  E+ A KRL   HG++ +D+S     +   K + +
Sbjct: 1   MDFLAKYQFDFNTCIHEGISYLSRREEEEASKRLKMLHGEDGIDSSGETEEL---KLVRL 60

Query: 212 ADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIFFKMRPALTLSDFTSH 271
           ADVLF  RM+  L+EWR  LL   N   +    +N   +  ET+F  MRPAL+L  FTSH
Sbjct: 61  ADVLFAARMEKLLNEWRSGLLHGGNASSEFPRISNGSNQSMETVFHHMRPALSLKGFTSH 120

Query: 272 QTKLIQLVIKKHFEDLCFVVVDDGNANLQQLVVYVDSRDDKELLMQEVNCDRQEAAEMKI 331
           Q +++  V++KHF DL ++  +D +++ + +VVY DS  DKE LM+E   +R+  AE KI
Sbjct: 121 QLRVLNSVLRKHFGDLVYIHSNDKSSSSRDIVVYTDSDSDKENLMKEAKDERKRLAERKI 180

Query: 332 QSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEEFVSSIGKRFPLIMD 391
           QSAIGFR VIDLL+SEKKLI+GHNCFLDIAH++ KF+G LP TAE+FV+SI   FP I+D
Sbjct: 181 QSAIGFRQVIDLLASEKKLIVGHNCFLDIAHVYSKFVGPLPSTAEKFVASINSHFPYIVD 240

Query: 392 TKILLNGNGILQQRMKKSSTSLSSAFALLCPHIAFSSQSNPAF-NSGVQVEVQVDDLSSS 451
           TKILLN N +L QRMKKSSTSLSSAF+ LCP I FSS+S+ +F    V ++V++D++  S
Sbjct: 241 TKILLNVNPMLHQRMKKSSTSLSSAFSSLCPQIEFSSRSSDSFLQQRVNIDVEIDNVRCS 300

Query: 452 NWNSGDKHEAGYDAFMTGCVFAQACNLMGIDFVLPS--ENLADNEKLQKHINHLYLSWAS 511
           NWN+G KHEAGYDAFMTGC+FAQACN +G DF   S  ++ A NEKL+K+IN LYLSW  
Sbjct: 301 NWNAGGKHEAGYDAFMTGCIFAQACNHLGFDFKQHSQLDDFAQNEKLEKYINRLYLSWTR 360

Query: 512 GDIIDLSTGNRMTEPLRSNRSKRHQVKISFENIVLIWGFPANLNARKIKECISKVFGLAN 571
           GDIIDL TG+       SN       K  +ENIVLIW FP  L AR IKECI K FG A+
Sbjct: 361 GDIIDLRTGH-------SNADNWRVSKFKYENIVLIWNFPRKLKARGIKECICKAFGSAS 420

Query: 572 IVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLHPLTELFGAKSTHAAGYE 631
           + SVYH+D+SAVFV F   E+V +FL LK  LE  D P+SVLHPL+++    +T AA YE
Sbjct: 421 VTSVYHVDDSAVFVLFKNSELVWDFLALKRQLESSDGPVSVLHPLSKILEGGNTGAADYE 480

Query: 632 AYKEICSSPMFKVLFADQAEAVGINQKT 657
           AYKEICSS + +V+F+DQAE VG+  +T
Sbjct: 481 AYKEICSSHVSEVMFSDQAETVGVKSRT 498

BLAST of CmaCh16G009590 vs. TAIR 10
Match: AT3G25430.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 226.1 bits (575), Expect = 8.9e-59
Identity = 176/639 (27.54%), Postives = 297/639 (46.48%), Query Frame = 0

Query: 24  SAAVTAPATASNSSFPLKNVTKSNFEAVLSELRSHVRAADFVAIDLEMTGITSAPWRESF 83
           SA      T +   + +K V KSNF   L E+R+ + ++DF+A+ L+ TG  +A W    
Sbjct: 10  SATAGNTKTLNQGRWSVKQVKKSNFHVTLDEIRTSIDSSDFIALSLQNTGSYAAAWHRVS 69

Query: 84  EFDRFDVRYLKVKDSAEKFAVVQFGVCPFRWDSAKNSFVAHPHNFFVFPRHEIQVDGPSS 143
             D     YLK K +AE++ ++QF +CPF    +K     HP+NF +FPR E++   PS 
Sbjct: 70  AIDTPQTSYLKAKYAAERYQILQFALCPFSLQGSK--LTVHPYNFHLFPRDELKCGMPSY 129

Query: 144 EFLCQTTSIEFLAKYQFDFNACIHEGISYLSRAHEDAARKRLNFAHGDETLDASSNVTNI 203
            F CQ + +  +A+  FDFN CI+EGISYLSRA E A++    F   +  L  S  V++ 
Sbjct: 130 SFSCQASRLTAMAREGFDFNICIYEGISYLSRAQESASK----FLSENPILADSVTVSS- 189

Query: 204 RNQKRIGIADVLFKERMKNRLHEWRDRLLSERNMECQGQEGANDYQRQFETIF-----FK 263
                  +AD +F  R+++R+  WR   +     +   + G +D       +      + 
Sbjct: 190 ---SPATVADTVFVGRIRSRVKNWRQSCI-----DSGSKTGDDDLVSSLRRLVLGSEQYG 249

Query: 264 MRPALTLSDFTSHQTKLIQLVIKKHFEDLCFVVVDDGNANLQQL-VVYVDSRDDKELLMQ 323
            R  LT+   +  Q +LI  ++ +  +D+  ++V   +   Q +  V++ S++DK+L  +
Sbjct: 250 SRLCLTIDVCSERQVQLILEMLTEFSDDVVPLLVASKSRGTQAVRTVFMSSKEDKDLFKR 309

Query: 324 EVNCDRQEAAEMKIQSAIGFRHVIDLLSSEKKLIIGHNCFLDIAHIHKKFIGSLPLTAEE 383
           E+  D ++    +++   GFR V+D +SS +K ++  N   D   IH KF+G LP   ++
Sbjct: 310 ELK-DLEKEENRRVR---GFREVVDFISSSQKPVVSQNYLSDFTSIHAKFLGPLPSNVDD 369

Query: 384 FVSSIGKRFPLIMDTKILLNGNGILQ--QRMKKSSTSLSSAFALLCPHIAFSSQSNPAFN 443
           F SS+   FP ++D    +     L     +  + +SL+  FA +   +A         N
Sbjct: 370 FSSSLSSAFPNVVDLSQFMKEISPLSNISNLPAAMSSLNRFFAPVDVEVA---------N 429

Query: 444 SGVQVEVQVDDLSSSNWNSGDKHEA-GYDAFMTGCVFAQACNLMGIDFVLPSENLADNEK 503
            G  V++             + H++ G +A M   +FA+ C +   D       +  NE 
Sbjct: 430 QGCPVKLD------------EGHQSHGQNAVMISQLFAKLCTIQKSDL----STIQSNED 489

Query: 504 LQKHINHLYLSWASGDIIDLSTGNRMTEPLR--SNRSKRHQVKISFENIVLIWGFPANLN 563
            Q        S    + +   + N   E ++  S  S+R    +S EN+V IWG    + 
Sbjct: 490 FQ-----ALASDEHANSVTSCSKNAGDENVKVWSKNSRR----VSSENLVFIWGLGKKMT 549

Query: 564 ARKIKECISKVFGL-ANIVSVYHIDESAVFVQFAKPEMVSEFLDLKENLERHDDPISVLH 623
           A K+K  + K   + A    V +ID S+  + F +      FL    N E+ D  +  + 
Sbjct: 550 AAKLKNVLQKSHPVFAREFDVKYIDRSSAILVFWESGPSETFLSAVNNEEQLDGSLREM- 585

Query: 624 PLTELFGAKSTHAAGYEAYKEICSSPMFKVLFADQAEAV 651
                  A+    AGYE YK  C    ++   AD AE++
Sbjct: 610 ------VAEGLRGAGYETYKRACRLGFWE---ADLAESL 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LG262.5e-21558.15Poly(A)-specific ribonuclease PARN OS=Arabidopsis thaliana OX=3702 GN=PARN PE=1 ... [more]
Q8W4C31.3e-5727.54Poly(A)-specific ribonuclease PARN-like OS=Arabidopsis thaliana OX=3702 GN=At3g2... [more]
Q90ZA15.3e-4026.25Poly(A)-specific ribonuclease PARN OS=Xenopus laevis OX=8355 GN=parn PE=1 SV=1[more]
Q7ZU929.0e-4026.35Poly(A)-specific ribonuclease PARN OS=Danio rerio OX=7955 GN=parn PE=1 SV=2[more]
P693412.5e-3724.38Poly(A)-specific ribonuclease PARN OS=Bos taurus OX=9913 GN=PARN PE=1 SV=2[more]
Match NameE-valueIdentityDescription
AT1G55870.11.8e-21658.15Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G55870.21.9e-15755.71Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G25430.18.9e-5927.54Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 217..237
NoneNo IPR availablePANTHERPTHR15092POLY A -SPECIFIC RIBONUCLEASE/TARGET OF EGR1, MEMBER 1coord: 38..655
NoneNo IPR availablePANTHERPTHR15092:SF22POLY(A)-SPECIFIC RIBONUCLEASE PNLDC1coord: 38..655
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 42..213
e-value: 6.5E-43
score: 149.0
coord: 245..518
e-value: 4.5E-45
score: 156.1
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 43..471
e-value: 1.1E-86
score: 291.1
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 40..481

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009590.1CmaCh16G009590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0005737 cytoplasm
molecular_function GO:0000175 3'-5'-exoribonuclease activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding