CmaCh16G009420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G009420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProtein kinase domain-containing protein
LocationCma_Chr16: 7260086 .. 7261353 (-)
RNA-Seq ExpressionCmaCh16G009420
SyntenyCmaCh16G009420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAACGCCAATAAATCGCCCACTTCCGATCCCTTCACCTCTCCCACTTTTCATGTTCATCTCTCAAGATTTCAATCCATTTTCCCTTCAACTTCATCACCCACTTCTTCATGGTATCAAATTTCAATCGGTTCCGCGTTCGAATTCTCAACTTTTCTTGTTCTTGAATTTCTCGTGATATTTGAATCTTTTGTTTCAGATGCCTAGTGTAACGACGGAGTCGTCCGAGAAGGACTCGGAGCCATTTGTAGAAGTGAATCCGACGGGTCGATACGGGCGATATGGCGAGCTTCTAGGGTCGGGAGCGGTGAAGAAAGTGTACCGAGCATTTGATCAAGAAGAAGGAATTGAGGTGGCTTGGAATCAAGTGAAATTAAGAAGCTTTTCAAATGATCCATCCATGATTGATAGGCTTTACTCGGAGGTGACATTGCTAAGAACCTTAAAGAACAATAACATCATTGCTTTGTACGATGTATGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACCGAGGTTTGTACAAGTGGGAATCTTAGAGAATATAGGAAGAAACATAGGCAAGTTTCTTTGAAAGCCTTGAAGAAATGGTGTAAGCAAATTCTTAAGGGTTTGCATTATTTGCATACTCATGAGCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTCAATGGCAATGTTGGGCAGGTACTCATTCATGAACTTGGGCTAATCTTGAGCTTAATAATTGGCTTAATTTTAGGGTTTTCTCATGATTTTGGCTACAAATTTGTTGGTCATGGTAGGTGAAGATTGGTGATTTGGGTATGGCTGCAACGGTGGGGAAGAATCACTCAGCTCATTCAGTGCTTGGGACACCGGAATTCATGGCACCTGAGCTATATGAAGAGCATTACACCGAGCTTGTGGACATATATTCATTTGGCATGTGTTTGCTTGAATTGGTCACCTTGGAAATTCCCTATAGTGAATGTGATAACGTTGCGAAAATCTATAAGAAAGTATGCTCGGGCATCAAGCCGTTGGCACTCGACAAGGTCAAGGACCTTGAGGTGAAGGCTTTCATTGAGAATTGCCTTGCTCCGTTCCAAGATCGGCCCTCAGCTGCCGACCTCCTCCGCCACCCGTTTTTCAGTGAAATTGACGACGACAACGACGATGAGGATAGTAATGATCGTTAGTGTATTGCGATTGAATAAGAATTTATATGTGGTTTGTATGTATGATGAATGAAATTAGTGTTTGTTTTCTCA

mRNA sequence

TCAACGCCAATAAATCGCCCACTTCCGATCCCTTCACCTCTCCCACTTTTCATGTTCATCTCTCAAGATTTCAATCCATTTTCCCTTCAACTTCATCACCCACTTCTTCATGATGCCTAGTGTAACGACGGAGTCGTCCGAGAAGGACTCGGAGCCATTTGTAGAAGTGAATCCGACGGGTCGATACGGGCGATATGGCGAGCTTCTAGGGTCGGGAGCGGTGAAGAAAGTGTACCGAGCATTTGATCAAGAAGAAGGAATTGAGGTGGCTTGGAATCAAGTGAAATTAAGAAGCTTTTCAAATGATCCATCCATGATTGATAGGCTTTACTCGGAGGTGACATTGCTAAGAACCTTAAAGAACAATAACATCATTGCTTTGTACGATGTATGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACCGAGGTTTGTACAAGTGGGAATCTTAGAGAATATAGGAAGAAACATAGGCAAGTTTCTTTGAAAGCCTTGAAGAAATGGTGTAAGCAAATTCTTAAGGGTTTGCATTATTTGCATACTCATGAGCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTCAATGGCAATGTTGGGCAGGTGAAGATTGGTGATTTGGGTATGGCTGCAACGGTGGGGAAGAATCACTCAGCTCATTCAGTGCTTGGGACACCGGAATTCATGGCACCTGAGCTATATGAAGAGCATTACACCGAGCTTGTGGACATATATTCATTTGGCATGTGTTTGCTTGAATTGGTCACCTTGGAAATTCCCTATAGTGAATGTGATAACGTTGCGAAAATCTATAAGAAAGTATGCTCGGGCATCAAGCCGTTGGCACTCGACAAGGTCAAGGACCTTGAGGTGAAGGCTTTCATTGAGAATTGCCTTGCTCCGTTCCAAGATCGGCCCTCAGCTGCCGACCTCCTCCGCCACCCGTTTTTCAGTGAAATTGACGACGACAACGACGATGAGGATAGTAATGATCGTTAGTGTATTGCGATTGAATAAGAATTTATATGTGGTTTGTATGTATGATGAATGAAATTAGTGTTTGTTTTCTCA

Coding sequence (CDS)

ATGATGCCTAGTGTAACGACGGAGTCGTCCGAGAAGGACTCGGAGCCATTTGTAGAAGTGAATCCGACGGGTCGATACGGGCGATATGGCGAGCTTCTAGGGTCGGGAGCGGTGAAGAAAGTGTACCGAGCATTTGATCAAGAAGAAGGAATTGAGGTGGCTTGGAATCAAGTGAAATTAAGAAGCTTTTCAAATGATCCATCCATGATTGATAGGCTTTACTCGGAGGTGACATTGCTAAGAACCTTAAAGAACAATAACATCATTGCTTTGTACGATGTATGGCTTGACAAACTTCATGGAACCTTAAATTTCATTACCGAGGTTTGTACAAGTGGGAATCTTAGAGAATATAGGAAGAAACATAGGCAAGTTTCTTTGAAAGCCTTGAAGAAATGGTGTAAGCAAATTCTTAAGGGTTTGCATTATTTGCATACTCATGAGCCTTGTGTTATTCATAGAGATCTCAATTGTAGCAACCTCTTTGTCAATGGCAATGTTGGGCAGGTGAAGATTGGTGATTTGGGTATGGCTGCAACGGTGGGGAAGAATCACTCAGCTCATTCAGTGCTTGGGACACCGGAATTCATGGCACCTGAGCTATATGAAGAGCATTACACCGAGCTTGTGGACATATATTCATTTGGCATGTGTTTGCTTGAATTGGTCACCTTGGAAATTCCCTATAGTGAATGTGATAACGTTGCGAAAATCTATAAGAAAGTATGCTCGGGCATCAAGCCGTTGGCACTCGACAAGGTCAAGGACCTTGAGGTGAAGGCTTTCATTGAGAATTGCCTTGCTCCGTTCCAAGATCGGCCCTCAGCTGCCGACCTCCTCCGCCACCCGTTTTTCAGTGAAATTGACGACGACAACGACGATGAGGATAGTAATGATCGTTAG

Protein sequence

MMPSVTTESSEKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIKPLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDEDSNDR
Homology
BLAST of CmaCh16G009420 vs. ExPASy Swiss-Prot
Match: Q6ICW6 (Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 GN=WNK11 PE=2 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 6.8e-130
Identity = 228/304 (75.00%), Postives = 265/304 (87.17%), Query Frame = 0

Query: 1   MMPSVTTESSE----KDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN 60
           MM   +++ +E    KDSE FVEV+PTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN
Sbjct: 1   MMTCASSDDNESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN 60

Query: 61  QVKLRSFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLR 120
           QVKLR FS+DP+M +RLYSEV LL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLR
Sbjct: 61  QVKLRCFSDDPAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLR 120

Query: 121 EYRKKHRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLG 180
           EYRKKHR VS++ALKKW KQILKGL YLHTH+PC+IHRDLNCSN+FVNGN+GQVKIGDLG
Sbjct: 121 EYRKKHRHVSMRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLG 180

Query: 181 MAATVGKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVA 240
           +AA VGKNH AHS+LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VA
Sbjct: 181 LAAIVGKNHLAHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVA 240

Query: 241 KIYKKVCSGIKPLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEI-DDDNDDE 300
           KIYK+V  G+KP AL+KV D E KAFIE C+A  + RPSAA+LL  PFF  I DDD++D 
Sbjct: 241 KIYKRVSKGLKPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDEDG 300

BLAST of CmaCh16G009420 vs. ExPASy Swiss-Prot
Match: Q0D541 (Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK5 PE=2 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 6.6e-109
Identity = 184/285 (64.56%), Postives = 238/285 (83.51%), Query Frame = 0

Query: 9   SSEKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSN-DP 68
           S+ ++ E F EV+PTGR+GRY ++LG G+VKKVYR FDQEEGIEVAWN+V+LR+ ++ DP
Sbjct: 37  SAGEEEEAFEEVDPTGRFGRYADVLGLGSVKKVYRGFDQEEGIEVAWNRVRLRALADRDP 96

Query: 69  SMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSL 128
           +M++RL++EV LLR+L + +II  + VWLD+  G LNFITEVCTSG+LREYR +HR VS+
Sbjct: 97  AMVERLHAEVRLLRSLHHEHIIGFHKVWLDRDAGVLNFITEVCTSGSLREYRDRHRHVSV 156

Query: 129 KALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSA 188
           KALKKW +QIL GL +LHTH+PC+IHRDLNCSN+F+NGN GQVKIGDLG+AA V K H A
Sbjct: 157 KALKKWARQILLGLDHLHTHDPCIIHRDLNCSNVFINGNTGQVKIGDLGLAAIVDKTHVA 216

Query: 189 HSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIK 248
           H++LGTPEFMAPELY E YTE VDIYS+GMC+LE+VT E+PY+ECD+V +IY  V  G+ 
Sbjct: 217 HTILGTPEFMAPELYTETYTESVDIYSYGMCVLEMVTREMPYAECDSVVQIYHSVTRGVP 276

Query: 249 PLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDN 293
           P AL +++D E++AFIE C+   ++RPSAA+LLR PFF+ IDDD+
Sbjct: 277 PAALKRIRDPELRAFIERCIGQPRNRPSAAELLRDPFFAGIDDDD 321

BLAST of CmaCh16G009420 vs. ExPASy Swiss-Prot
Match: Q9STK6 (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN=WNK3 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 8.1e-99
Identity = 181/291 (62.20%), Postives = 218/291 (74.91%), Query Frame = 0

Query: 8   ESSEKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDP 67
           +      E FVE++PTGRYGRY E+LG GA K+VYRAFDQ EGIEVAWNQVKL       
Sbjct: 3   QDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSS 62

Query: 68  SMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSL 127
             +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V L
Sbjct: 63  EDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDL 122

Query: 128 KALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSA 187
           +ALKKW +QIL+GL YLH+H+P VIHRDL C N+F+NGN G+VKIGDLG+AA + +  SA
Sbjct: 123 RALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSA 182

Query: 188 HSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIK 247
           HSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV SGIK
Sbjct: 183 HSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIK 242

Query: 248 PLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDEDSN 299
           P AL  V D +V+AFIE C+A    R SA +LL  PF     ++ ++  S+
Sbjct: 243 PAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLKCYKENTENVSSH 293

BLAST of CmaCh16G009420 vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-98
Identity = 181/289 (62.63%), Postives = 220/289 (76.12%), Query Frame = 0

Query: 11  EKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           E D   FVEV+PTGRYGRY E+LG GA K VYRAFD+ EGIEVAWNQVKL  F   P  +
Sbjct: 8   EPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDL 67

Query: 71  DRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLY E+ LL+TLK+ NI+  Y  W+D  +  +NF+TE+ TSG LR+YR +H++V+++A+
Sbjct: 68  ERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAM 127

Query: 131 KKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSAHSV 190
           K WC+QIL+GLHYLH+H+P VIHRDL C N+FVNGN G+VKIGDLG+AA + K+H+AH V
Sbjct: 128 KHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV 187

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIKPLA 250
            GTPEFMAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP A
Sbjct: 188 -GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDA 247

Query: 251 LDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDEDSND 300
           L KVKD EVK FIE CLA    R SA +LL  PF   IDD   D  S D
Sbjct: 248 LYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFL-RIDDGEFDLRSVD 294

BLAST of CmaCh16G009420 vs. ExPASy Swiss-Prot
Match: Q65X23 (Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK2 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.8e-96
Identity = 173/288 (60.07%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 1   MMPSVTTESSEKDSEP-FVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVK 60
           M P+   E    D+EP F EV+PT RYGRY E+LG GA K VY+AFDQ EG+EVAWNQ+K
Sbjct: 1   MPPTPPPELDLLDTEPEFAEVDPTARYGRYTEVLGKGAFKTVYKAFDQLEGLEVAWNQIK 60

Query: 61  LRSFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYR 120
           +     +   ++RL SEV LL+TLK+ NII  Y+ WLDK +  +NFITEV TSG LR+YR
Sbjct: 61  VGDILRNNDDLERLRSEVRLLKTLKHKNIIKFYNSWLDKKNNNINFITEVFTSGTLRQYR 120

Query: 121 KKHRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAA 180
            KH++V ++ALKKW +QIL GL YLH+H+P VIHRDL C N+FVNGN G+VKIGDLG+A 
Sbjct: 121 IKHKKVDVRALKKWSRQILSGLVYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAT 180

Query: 181 TVGKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIY 240
            +    SAHS++GTPEFMAPELY+E Y ELVDIY+FGMCLLELVT E PY EC N A+IY
Sbjct: 181 ILDNARSAHSIIGTPEFMAPELYDEEYNELVDIYAFGMCLLELVTFEYPYCECSNAAQIY 240

Query: 241 KKVCSGIKPLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSE 288
           KKV  G KP +L K++D EV+ FIE C+A    R SA +LL  PF  +
Sbjct: 241 KKVSDGEKPSSLAKIEDPEVRFFIEKCIAKASQRLSAQELLMDPFLRD 288

BLAST of CmaCh16G009420 vs. TAIR 10
Match: AT5G55560.1 (Protein kinase superfamily protein )

HSP 1 Score: 464.9 bits (1195), Expect = 4.8e-131
Identity = 228/304 (75.00%), Postives = 265/304 (87.17%), Query Frame = 0

Query: 1   MMPSVTTESSE----KDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN 60
           MM   +++ +E    KDSE FVEV+PTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN
Sbjct: 1   MMTCASSDDNESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWN 60

Query: 61  QVKLRSFSNDPSMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLR 120
           QVKLR FS+DP+M +RLYSEV LL+ LKN+NII LY VW D+ + TLNFITE+CTSGNLR
Sbjct: 61  QVKLRCFSDDPAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLR 120

Query: 121 EYRKKHRQVSLKALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLG 180
           EYRKKHR VS++ALKKW KQILKGL YLHTH+PC+IHRDLNCSN+FVNGN+GQVKIGDLG
Sbjct: 121 EYRKKHRHVSMRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLG 180

Query: 181 MAATVGKNHSAHSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVA 240
           +AA VGKNH AHS+LGTPEFMAPELYEE+YTE+VDIYS+GMC+LELV+LEIPYSECD+VA
Sbjct: 181 LAAIVGKNHLAHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVA 240

Query: 241 KIYKKVCSGIKPLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEI-DDDNDDE 300
           KIYK+V  G+KP AL+KV D E KAFIE C+A  + RPSAA+LL  PFF  I DDD++D 
Sbjct: 241 KIYKRVSKGLKPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDEDG 300

BLAST of CmaCh16G009420 vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 361.7 bits (927), Expect = 5.7e-100
Identity = 181/291 (62.20%), Postives = 218/291 (74.91%), Query Frame = 0

Query: 8   ESSEKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDP 67
           +      E FVE++PTGRYGRY E+LG GA K+VYRAFDQ EGIEVAWNQVKL       
Sbjct: 3   QDENNSEEEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSS 62

Query: 68  SMIDRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSL 127
             +DRLYSEV LL+TLK+ +II  Y  W+D  H T+N ITEV TSGNLR+YRKKH+ V L
Sbjct: 63  EDLDRLYSEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDL 122

Query: 128 KALKKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSA 187
           +ALKKW +QIL+GL YLH+H+P VIHRDL C N+F+NGN G+VKIGDLG+AA + +  SA
Sbjct: 123 RALKKWSRQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSA 182

Query: 188 HSVLGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIK 247
           HSV+GTPEFMAPELYEE Y  LVDIY+FGMCLLELVT E PYSEC N A+IY+KV SGIK
Sbjct: 183 HSVIGTPEFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIK 242

Query: 248 PLALDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDEDSN 299
           P AL  V D +V+AFIE C+A    R SA +LL  PF     ++ ++  S+
Sbjct: 243 PAALLNVTDPQVRAFIEKCIAKVSQRLSAKELLDDPFLKCYKENTENVSSH 293

BLAST of CmaCh16G009420 vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 360.9 bits (925), Expect = 9.8e-100
Identity = 181/289 (62.63%), Postives = 220/289 (76.12%), Query Frame = 0

Query: 11  EKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           E D   FVEV+PTGRYGRY E+LG GA K VYRAFD+ EGIEVAWNQVKL  F   P  +
Sbjct: 8   EPDYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDL 67

Query: 71  DRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLY E+ LL+TLK+ NI+  Y  W+D  +  +NF+TE+ TSG LR+YR +H++V+++A+
Sbjct: 68  ERLYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAM 127

Query: 131 KKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSAHSV 190
           K WC+QIL+GLHYLH+H+P VIHRDL C N+FVNGN G+VKIGDLG+AA + K+H+AH V
Sbjct: 128 KHWCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAHCV 187

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIKPLA 250
            GTPEFMAPE+YEE Y ELVDIYSFGMC+LE+VT + PYSEC + A+IYKKV SG KP A
Sbjct: 188 -GTPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDA 247

Query: 251 LDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDDNDDEDSND 300
           L KVKD EVK FIE CLA    R SA +LL  PF   IDD   D  S D
Sbjct: 248 LYKVKDPEVKCFIEKCLATVSLRVSARELLDDPFL-RIDDGEFDLRSVD 294

BLAST of CmaCh16G009420 vs. TAIR 10
Match: AT5G28080.2 (Protein kinase superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 3.3e-95
Identity = 165/281 (58.72%), Postives = 213/281 (75.80%), Query Frame = 0

Query: 11  EKDSEPFVEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMI 70
           E D   +VEV+PTGRYGRY E+LG G+ K VYR FD+ +GIEVAWNQVKL  F   P  +
Sbjct: 9   ESDYSEYVEVDPTGRYGRYNEVLGKGSSKTVYRGFDEYQGIEVAWNQVKLYDFLQSPQEL 68

Query: 71  DRLYSEVTLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKAL 130
           +RLY E+ LL+TLK+ +I+  Y  W+D  +  +NF+TE+ TSG LR+YR KH++V+++A+
Sbjct: 69  ERLYCEIHLLKTLKHKSIMKFYASWVDTDNRNINFVTEMFTSGTLRQYRLKHKRVNIRAV 128

Query: 131 KKWCKQILKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSAHSV 190
           K WC+QIL+GL+YLHTH+P VIHRDL C N+F+NGN G+VKIGDLG+AA +  +H+AH V
Sbjct: 129 KNWCRQILRGLNYLHTHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAACLQHSHAAHCV 188

Query: 191 LGTPEFMAPELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIKPLA 250
            GTPEFMAPE+Y+E Y +LVDIYSFGMC+LE+VT + PYSEC + A+IYK+V SG KP  
Sbjct: 189 -GTPEFMAPEVYKEEYNQLVDIYSFGMCVLEMVTFDYPYSECSHPAQIYKRVISGKKPDG 248

Query: 251 LDKVKDLEVKAFIENCLAPFQDRPSAADLLRHPFFSEIDDD 292
           LDKVKD EV+ FIE CLA    R SA +LL   F    + D
Sbjct: 249 LDKVKDPEVRGFIEKCLATVSLRLSACELLDDHFLCIDESD 288

BLAST of CmaCh16G009420 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 341.3 bits (874), Expect = 8.0e-94
Identity = 164/267 (61.42%), Postives = 208/267 (77.90%), Query Frame = 0

Query: 18  VEVNPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRSFSNDPSMIDRLYSEV 77
           +EV+PT RY RY E++G GA K VY+AFD+ +GIEVAWNQV++      P+ ++RLYSEV
Sbjct: 19  LEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEV 78

Query: 78  TLLRTLKNNNIIALYDVWLDKLHGTLNFITEVCTSGNLREYRKKHRQVSLKALKKWCKQI 137
            LL++LK+NNII  Y+ W+D  + T+N ITE+ TSG+LR YRKKHR+V++KA+K W +QI
Sbjct: 79  RLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQI 138

Query: 138 LKGLHYLHTHEPCVIHRDLNCSNLFVNGNVGQVKIGDLGMAATVGKNHSAHSVLGTPEFM 197
           L GL YLH  EP +IHRDL C N+F+NGN G+VKIGDLG+ ATV +  +A SV+GTPEFM
Sbjct: 139 LMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGL-ATVMEQANAKSVIGTPEFM 198

Query: 198 APELYEEHYTELVDIYSFGMCLLELVTLEIPYSECDNVAKIYKKVCSGIKPLALDKVKDL 257
           APELY+E+Y EL DIYSFGMC+LE+VT + PY EC N A+IYKKV SGIKP +L +VKD 
Sbjct: 199 APELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIKPASLSRVKDP 258

Query: 258 EVKAFIENCLAPFQDRPSAADLLRHPF 285
           EVK FIE CL P  +R SA +LL  PF
Sbjct: 259 EVKQFIEKCLLPASERLSAKELLLDPF 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6ICW66.8e-13075.00Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 G... [more]
Q0D5416.6e-10964.56Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX... [more]
Q9STK68.1e-9962.20Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9CAV61.4e-9862.63Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=... [more]
Q65X233.8e-9660.07Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX... [more]
Match NameE-valueIdentityDescription
AT5G55560.14.8e-13175.00Protein kinase superfamily protein [more]
AT3G48260.15.7e-10062.20with no lysine (K) kinase 3 [more]
AT3G04910.19.8e-10062.63with no lysine (K) kinase 1 [more]
AT5G28080.23.3e-9558.72Protein kinase superfamily protein [more]
AT3G18750.18.0e-9461.42with no lysine (K) kinase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 31..285
e-value: 1.6E-50
score: 171.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 27..285
score: 39.22681
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 10..108
e-value: 6.7E-28
score: 98.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 110..291
e-value: 1.7E-49
score: 169.8
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 28..240
e-value: 5.1E-25
score: 85.8
NoneNo IPR availablePANTHERPTHR13902:SF119SERINE/THREONINE-KINASE WNK11-LIKE PROTEINcoord: 2..299
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 2..299
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 25..285
e-value: 3.1514E-154
score: 429.723
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 29..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G009420.1CmaCh16G009420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity