CmaCh16G009310 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G009310
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectin lyase-like superfamily protein, putative
Location	Cma_Chr16: 7116300 .. 7117525 (+)
RNA-Seq Expression	CmaCh16G009310
Synteny	CmaCh16G009310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCATTAAGTAAGGCATGGAGGCAAGCTTGTTGGCGTGATGGAGGTGGTGTAGTTTTAGTACCAGATGGGAGGTTTCTAATTTTTTCCTTGGTGCTTAAAGGTCCATGCAAAGGATCCATTGGTCTACGGATTAATGGCCAATTGTTGGCTCCTATCGATCCAAATTTCGCCATTGCCCAAATCTGGTTGAGTATCTATGAAGTCGACAACTTATTTGTCGACGGGTATGGCAGCTTCGACGGTCGCGGTTTTTTAGCTTGGTCTATCAAAACCTTTAACCGTCCAATTGTAAGTCTATTTTTTTGGATTCTTTGGTTAAAGTGTTAGATGGAACCCTAATTTTCACTTCCTTTTTTTTTTTTTTAGTTATTTGCCAAGTGACCGTGAAATTGTTTCTATGTAGTCTATGAAACTCACTAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGCAAATTCTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACATTTGATCACATTAGAGTAATGGCTCCAAGAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGACACTGGAGACGATTGTATCTCTTTGGGCCCTGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGGTATATATATTCAAATTTGATATCGTTAGGTTTTAGTAAAAGTGAAAATGGTATTACAATGAATGTTGTTTGTGGTGGACAGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

mRNA sequence

GCATTAAGTAAGGCATGGAGGCAAGCTTGTTGGCGTGATGGAGGTGGTGTAGTTTTAGTACCAGATGGGAGGTTTCTAATTTTTTCCTTGGTGCTTAAAGGTCCATGCAAAGGATCCATTGGTCTACGGATTAATGGCCAATTGTTGGCTCCTATCGATCCAAATTTCGCCATTGCCCAAATCTGGTTGAGTATCTATGAAGTCGACAACTTATTTGTCGACGGGTATGGCAGCTTCGACGGTCGCGGTTTTTTAGCTTGGTCTATCAAAACCTTTAACCGTCCAATTTCTATGAAACTCACTAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGCAAATTCTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACATTTGATCACATTAGAGTAATGGCTCCAAGAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGACACTGGAGACGATTGTATCTCTTTGGGCCCTGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

Coding sequence (CDS)

GCATTAAGTAAGGCATGGAGGCAAGCTTGTTGGCGTGATGGAGGTGGTGTAGTTTTAGTACCAGATGGGAGGTTTCTAATTTTTTCCTTGGTGCTTAAAGGTCCATGCAAAGGATCCATTGGTCTACGGATTAATGGCCAATTGTTGGCTCCTATCGATCCAAATTTCGCCATTGCCCAAATCTGGTTGAGTATCTATGAAGTCGACAACTTATTTGTCGACGGGTATGGCAGCTTCGACGGTCGCGGTTTTTTAGCTTGGTCTATCAAAACCTTTAACCGTCCAATTTCTATGAAACTCACTAAAATCAACCATGCGAGAATTACGGGTATAACATCGTACAATAGCAAATTCTTCCATTTTGGTGTGCATGGATGTAGTGATGTCACATTTGATCACATTAGAGTAATGGCTCCAAGAGATAGCCCCAACACAGATGGAATCCACATTTCCAAGTCAAGTGGTATCAACATTGTGCATTCAAGATTCGACACTGGAGACGATTGTATCTCTTTGGGCCCTGGAAGTAGATTCATCAACATAACAAACATCCATTGTGGTCCAGGTCACGGAATCAGCATCGGCAGCCTTGGAAAATACCCAAATGAGGAAGATGTCTCTGACGTTTTAGTAAGAGACTCCACATTTGTTGGTACAGACAACGGTGTGAGGATCAAAACATGGTCGTCTTCACATCCTAGTTTTGTTACCAAGATTGTTTTTCTCAATCTCGAAATGAAGGACGTAAGAAACCCTATGATCATTGATCAAAAATATTGCCTTGACTGCAGCAGAGATAAAATGAGTACTTCAAGAGTGCAGATTCGAGACGTGAGATATGAAGGTATACGGGGGACTTCAGCAACCCAAGCGGCAGTAAATTTTGAATGCAGCCAAGTTTTTCCATGCGAAGGCATAGTGTTGCAAGACATTAACCTCTCCTTTAATGGTGGTGGCCAACCAACTTCCATTTGTCACAATGTGAAGGGCTCTGCTTTTGGCAAACAACTTCCTTCCTCTTGCCTTTAA

Protein sequence

ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQIWLSIYEVDNLFVDGYGSFDGRGFLAWSIKTFNRPISMKLTKINHARITGITSYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAAVNFECSQVFPCEGIVLQDINLSFNGGGQPTSICHNVKGSAFGKQLPSSCL

Homology

[Close]

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.6e-87
Identity = 153/348 (43.97%), Postives = 221/348 (63.51%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGS-IGLRINGQLLAPIDPNFAIA 60
           A+ KAW+ AC   G   VL+P G + +  + ++GPCKGS IG +I+G + AP DP+   +
Sbjct: 26  AVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKGSKIGFQIDGVVKAPADPSKFKS 85

Query: 61  QIWLSIYEVDNLFVDGYGSFDGRGFLAWSIKTFNR-------PISMKLTKINHARITGIT 120
             W+S Y +D L V G G+ DG+G  AW+    ++        ++++   + HA +  IT
Sbjct: 86  DGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPNCKHAAMNLRFDFLKHAMVRDIT 145

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
           S NSK FH  V  C D+TF H+ V AP  S NTDGIH+  S G+ I +++  TGDDCIS+
Sbjct: 146 SLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHVGISKGVTITNTKIATGDDCISI 205

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSH 240
           GPGS+ + IT ++CGPGHGISIGSLG+Y NE++V  + V+  TF GT NGVR+KTW +S 
Sbjct: 206 GPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGITVKGCTFSGTMNGVRVKTWPNSP 265

Query: 241 PSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAA 300
           P   T + F +L M +V+NP+I+DQ+YC      + + SR+++ ++ +  IRGTS  + A
Sbjct: 266 PGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAPSRIKLSNINFNNIRGTSTGKVA 325

Query: 301 VNFECSQVFPCEGIVLQDINLSFNGGGQP-TSICHNVKGSAFGKQLPS 340
           V   CS   PC  + + +INLS+ G G P TS C NVK +  GKQ+P+
Sbjct: 326 VVIACSHGMPCSNMKIGEINLSYRGAGGPATSTCSNVKPTFSGKQVPA 373

[Close]

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.8e-84
Identity = 152/350 (43.43%), Postives = 221/350 (63.14%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQ 60
           AL+ AW++AC       +LVP G F +  + L+GPCK SIGL++ G L AP DP+     
Sbjct: 5   ALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGL 64

Query: 61  IWLSIYEVDNLFVDGYGSFDGRGFLAWSIKTF--NRPI------SMKLTKINHARITGIT 120
            W+++ ++D L + G G FDG+G  AW       N PI      +++L  + ++ +  +T
Sbjct: 65  GWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNSILRDVT 124

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
           + +SK FH  V GC ++TF+  ++ A   S NTDGIHI +S G+NI+++   TGDDCISL
Sbjct: 125 TLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTGDDCISL 184

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSH 240
           G GS+ INITNI CGPGHGIS+GSLG+Y NEE V  + V++ T  G+ NGVRIKTW  S 
Sbjct: 185 GDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIKTWPKSE 244

Query: 241 PSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAA 300
           P   +++ F ++ M  V  P++IDQ YC          S V++  + ++ I+GTS T+ A
Sbjct: 245 PGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFKNIKGTSTTKEA 304

Query: 301 VNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGKQLPSSC 342
           V   CS+ FPC G+ L DI+L+++G GG  TS+C N+K +  GKQ+P+ C
Sbjct: 305 VKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAIC 354

[Close]

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.8e-79
Identity = 146/344 (42.44%), Postives = 210/344 (61.05%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPID-PNFAIA 60
           A+ +AW  AC   G   +L+P G FL+  L   GPCKG + +++NG LLA  D   +   
Sbjct: 59  AVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGDVTIQVNGNLLATTDLSQYKDH 118

Query: 61  QIWLSIYEVDNLFVDGYGSFDGRGFLAWS----IKTFN---RPISMKLTKINHARITGIT 120
             W+ I  VDNL + G G  DG+G   WS    +K ++    P S+ +  +N+  ++GIT
Sbjct: 119 GNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYDCKILPNSLVMDFVNNGEVSGIT 178

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
             NSKFFH  ++ C D+    + V AP DSPNTDGIH+  SSG+ I ++    GDDCIS+
Sbjct: 179 LLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHMGDSSGVTITNTVIGVGDDCISI 238

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSH 240
           GPG+  +NIT + CGPGHGISIGSLG+Y +E+DV+D+ V+D T   T NGVRIK +  + 
Sbjct: 239 GPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTANGVRIKAYEDAA 298

Query: 241 PSF-VTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
                +KI + N++M+D   P+IID KYC +        S+V ++DV ++ I GTS+T  
Sbjct: 299 SVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPE 358

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNGGGQPT-SICHNVKGSAFG 335
           AVN  C+   PC G+ + D+N+ ++G    T ++C N KGSA G
Sbjct: 359 AVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNAKGSAKG 402

[Close]

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 146/351 (41.60%), Postives = 209/351 (59.54%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQ 60
           A + AW++AC    G  + VP G +++ SL  KGPCKG + L +NG   AP         
Sbjct: 88  AFAAAWKEAC--AAGSTITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 147

Query: 61  I-WLSIYEVDNLFVDGYGS-FDGRGFLAWSI-------KTFNRPISMKLTKINHARITGI 120
             W+    + +  ++G  + FDG+G LAW         K  + PI+++ T + +++I  I
Sbjct: 148 AGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSI 207

Query: 121 TSYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCIS 180
           TS NSK FH  +  C ++T   I + AP +S NTDGIHI +S+G+N++ ++  TGDDC+S
Sbjct: 208 TSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVS 267

Query: 181 LGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSS 240
           +G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR      TDNGVRIKTW  S
Sbjct: 268 IGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS 327

Query: 241 HPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
            P   + I+F ++ M +V  P++IDQ+YC          S+V++ DV  +GI+GTSAT+ 
Sbjct: 328 PPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKV 387

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGKQLPSSC 342
           AV   CS+  PC  I L DINL  NG  G   S C N+K    GK +P++C
Sbjct: 388 AVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAAC 436

[Close]

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 2.0e-77
Identity = 143/344 (41.57%), Postives = 208/344 (60.47%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPID-PNFAIA 60
           A+ +AW  AC   G   +L+P G FL+  L   GPCKG + ++++G LLA  D   +   
Sbjct: 59  AVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGDVTIQVDGNLLATTDLSQYKDH 118

Query: 61  QIWLSIYEVDNLFVDGYGSFDGRGFLAWSIKTFNR-------PISMKLTKINHARITGIT 120
             W+ I  VDNL + G G+ DG+G   WS  +  +       P S+ +  +N+  ++G+T
Sbjct: 119 GNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYDCKILPNSLVMDFVNNGEVSGVT 178

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
             NSKFFH  ++ C D+    + V AP DSPNTDGIH+  SSGI I ++    GDDCIS+
Sbjct: 179 LLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHMGDSSGITITNTVIGVGDDCISI 238

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSH 240
           GPG+  +NIT + CGPGHGISIGSLG+Y +E+DV+D+ V+D T   T  GVRIK +  + 
Sbjct: 239 GPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDINVKDCTLKKTMFGVRIKAYEDAA 298

Query: 241 PSF-VTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
               V+KI + N++M+D  NP+ ID KYC +        S+V ++DV ++ I GTS+T  
Sbjct: 299 SVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANGASKVTVKDVTFKNITGTSSTPE 358

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNGGGQPT-SICHNVKGSAFG 335
           AV+  C+   PC G+ + D+N+ ++G    T +IC N KGS  G
Sbjct: 359 AVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNAKGSTKG 402

[Close]

BLAST of CmaCh16G009310 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 8.3e-79
Identity = 146/351 (41.60%), Postives = 209/351 (59.54%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQ 60
           A + AW++AC    G  + VP G +++ SL  KGPCKG + L +NG   AP         
Sbjct: 88  AFAAAWKEAC--AAGSTITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 147

Query: 61  I-WLSIYEVDNLFVDGYGS-FDGRGFLAWSI-------KTFNRPISMKLTKINHARITGI 120
             W+    + +  ++G  + FDG+G LAW         K  + PI+++ T + +++I  I
Sbjct: 148 AGWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSI 207

Query: 121 TSYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCIS 180
           TS NSK FH  +  C ++T   I + AP +S NTDGIHI +S+G+N++ ++  TGDDC+S
Sbjct: 208 TSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVS 267

Query: 181 LGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSS 240
           +G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR      TDNGVRIKTW  S
Sbjct: 268 IGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS 327

Query: 241 HPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
            P   + I+F ++ M +V  P++IDQ+YC          S+V++ DV  +GI+GTSAT+ 
Sbjct: 328 PPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIKGIKGTSATKV 387

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGKQLPSSC 342
           AV   CS+  PC  I L DINL  NG  G   S C N+K    GK +P++C
Sbjct: 388 AVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAAC 436

[Close]

BLAST of CmaCh16G009310 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.1e-78
Identity = 146/351 (41.60%), Postives = 208/351 (59.26%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQ 60
           A + AW++AC    G  + VP G +L+ SL  KGPCKG + L +NG   AP         
Sbjct: 89  AFAAAWKEAC--AAGSTITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 148

Query: 61  I-WLSIYEVDNLFVDGYGS-FDGRGFLAWSI-------KTFNRPISMKLTKINHARITGI 120
             W+    + +  ++G  + FDG+G LAW         K  + PI+++ T + +++I  I
Sbjct: 149 AGWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPINIRFTGLTNSKINSI 208

Query: 121 TSYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCIS 180
           TS NSK FH  +  C ++T   I + AP +S NTDGIHI +S+G+N++ ++  TGDDC+S
Sbjct: 209 TSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDCVS 268

Query: 181 LGPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSS 240
           +G G+  + + N+ CGPGHGISIGSLG+YPNE+ V  V VR      TDNGVRIKTW  S
Sbjct: 269 IGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGS 328

Query: 241 HPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
            P   + I+F ++ M +V  P++IDQ+YC          S+V++ DV  + I+GTSAT+ 
Sbjct: 329 PPGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIKNIKGTSATKV 388

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGKQLPSSC 342
           AV   CS+  PC  I L DINL  NG  G   S C N+K    GK +P++C
Sbjct: 389 AVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAAC 437

[Close]

BLAST of CmaCh16G009310 vs. TAIR 10
Match: AT2G26620.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 283.9 bits (725), Expect = 1.7e-76
Identity = 150/353 (42.49%), Postives = 205/353 (58.07%), Query Frame = 0


Query: 1   ALSKAWRQACWR-DGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIA 60
           A +  W +AC R  G   + VP G F +  +   GPCK  I   I+G LLAP +PN    
Sbjct: 49  AFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFIIDGTLLAPANPNDIKQ 108

Query: 61  QIWLSIYEVDNLFVDGYGSFDGRGFLAWSI----KTFNRP---ISMKLTKINHARITGIT 120
             W++   ++NL + G G+ DG+G  +W +    K  N P   ISM    +N++ I  IT
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKQSWPLNDCHKNPNCPKLAISMGFAFVNNSNIKDIT 168

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
           S NSK  HF            + + AP DSPNTDGI +   S I I ++   TGDDCI++
Sbjct: 169 SLNSKMGHFNFFFVHHFNITGVTITAPSDSPNTDGIKMGSCSNIQISNTNIGTGDDCIAI 228

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTW-SSS 240
             G+  +NI+NI+CGPGHGIS+GSLGK  +E+DV D+ VRD  F GT +G+RIKTW SS+
Sbjct: 229 LSGTTKLNISNINCGPGHGISVGSLGKNKDEKDVKDLFVRDVIFNGTSDGIRIKTWESSA 288

Query: 241 HPSFVTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQA 300
               V+  V+ N++M DV  P+ IDQKYC     +    S VQI+D++ + I GTS  + 
Sbjct: 289 SKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCEHERKSHVQIQDLKLKNIYGTSKNKV 348

Query: 301 AVNFECSQVFPCEGIVLQDINLSFNG--GGQPTSICHNVKGSAFGKQLPSSCL 343
           AVN +CS+ FPC+ + L DIN+  NG   G   ++C NV G A GK  P  CL
Sbjct: 349 AVNLQCSKSFPCKNVELIDINIKQNGLEDGSSITVCENVDGFARGKMFPQHCL 401

[Close]

BLAST of CmaCh16G009310 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.9e-76
Identity = 147/351 (41.88%), Postives = 214/351 (60.97%), Query Frame = 0


Query: 1   ALSKAWRQACWRDGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIAQ 60
           A + AW+ AC  +G   + +P G F +  +   GPC G I   I+G LLAP + +    +
Sbjct: 51  AFTNAWKDACRWNGPSKMYIPLGTFYLGGVTFVGPCDGKISFVIDGTLLAPPNNDDIKKE 110

Query: 61  IWLSIYEVDNLFVDGYGSFDGRGFLAWSI----KTFNRP---ISMKLTKINHARITGITS 120
           IW++   ++ L V G G+ DG+G  +WS+    K  N P   I+M    + ++ + GITS
Sbjct: 111 IWINFRYINYLTVFGDGTLDGQGKKSWSLIDCQKDNNCPKLAINMGFDFVKNSSMNGITS 170

Query: 121 YNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISLG 180
            NSK  HF        +   + ++AP +SPNTDGI I+ SS + I ++   TGDDCI++ 
Sbjct: 171 LNSKAGHFNFLSVDHFSITRVNIIAPSNSPNTDGIKIALSSNMQISNTHISTGDDCIAML 230

Query: 181 PGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSHP 240
            G+   +I N+ CGPGHGISIGSLGK  +E++V+ ++VR+S F GT NG+RIKTW SS  
Sbjct: 231 SGNTNFDIYNVKCGPGHGISIGSLGKNKDEKNVNGLMVRNSVFTGTTNGIRIKTWESSAS 290

Query: 241 SF-VTKIVFLNLEMKDVRNPMIIDQKYCLDCSRDKMSTSRVQIRDVRYEGIRGTSATQAA 300
           +  +  +V+ NL+M +V NP+ IDQKYC       M  S +QIR+V  + I GTS  + A
Sbjct: 291 TIRIINLVYENLQMINVENPIGIDQKYCPYPPCSNMGDSHIQIRNVTLKNIWGTSKNKVA 350

Query: 301 VNFECSQVFPCEGIVLQDINLSFNG-GGQPTSICHNVKGSAFGKQLPSSCL 343
           V F+CS+ FPC+ + L DINL+ +G  G  +++C NV GSA GK +P  CL
Sbjct: 351 VKFQCSKTFPCKDVQLIDINLTHHGVDGPASALCENVDGSATGKMVPPHCL 401

[Close]

BLAST of CmaCh16G009310 vs. TAIR 10
Match: AT1G43080.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 282.7 bits (722), Expect = 3.9e-76
Identity = 147/355 (41.41%), Postives = 206/355 (58.03%), Query Frame = 0


Query: 1   ALSKAWRQACWR-DGGGVVLVPDGRFLIFSLVLKGPCKGSIGLRINGQLLAPIDPNFAIA 60
           A +  W++AC R  G   + VP G F +  +   GPCK  I   I+G LLAP +P     
Sbjct: 49  AFTSVWKRACTRISGSSKIYVPKGTFYLGGVEFVGPCKNPIEFVIDGTLLAPANPRDIKQ 108

Query: 61  QIWLSIYEVDNLFVDGYGSFDGRGFLAWSI----KTFNRP---ISMKLTKINHARITGIT 120
             W++   ++NL + G G+ DG+G  +W +    K  N P   ++M    +N++RI  IT
Sbjct: 109 DTWINFRYINNLSISGSGTLDGQGKYSWPLNDCHKNTNCPKLAMTMGFAFVNNSRIKDIT 168

Query: 121 SYNSKFFHFGVHGCSDVTFDHIRVMAPRDSPNTDGIHISKSSGINIVHSRFDTGDDCISL 180
           S NSK  HF            + + AP DSPNTDGI +   S I+I ++   TGDDCI++
Sbjct: 169 SLNSKMGHFNFFSVHRFNITGVTITAPGDSPNTDGIKMGSCSNIHISNTNIGTGDDCIAI 228

Query: 181 GPGSRFINITNIHCGPGHGISIGSLGKYPNEEDVSDVLVRDSTFVGTDNGVRIKTWSSSH 240
             G+  ++I+NI CGPGHGIS+GSLGK  +E+DV  + VRD+ F GT +G+RIKTW SS 
Sbjct: 229 LSGTTNLDISNIKCGPGHGISVGSLGKNKDEKDVKHLTVRDTVFNGTSDGIRIKTWESSA 288

Query: 241 PSF-VTKIVFLNLEMKDVRNPMIIDQKYC--LDCSRDKMSTSRVQIRDVRYEGIRGTSAT 300
               V+  ++ N++M DV  P+ IDQKYC    C  +K   S VQI+D++ + I GTS  
Sbjct: 289 SKIVVSNFIYENIQMIDVGKPINIDQKYCPHPPCEHEKKGESHVQIQDIKLKNIYGTSNN 348

Query: 301 QAAVNFECSQVFPCEGIVLQDINLSFNG--GGQPTSICHNVKGSAFGKQLPSSCL 343
             AVN +CS+ FPC+ + L DINL   G   G  T++C NV GS   K +P  CL
Sbjct: 349 IVAVNLQCSKSFPCKNVELIDINLKHTGLEKGHSTAMCENVDGSVRSKMVPQHCL 403

The following BLAST results are available for this feature:

BLAST of CmaCh16G009310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6H9K0	3.6e-87	43.97	Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S...	[more]
P24548	3.8e-84	43.43	Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1	[more]
P35339	2.8e-79	42.44	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1	[more]
P49063	1.2e-77	41.60	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ...	[more]
P26216	2.0e-77	41.57	Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1	[more]

BLAST of CmaCh16G009310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07850.1	8.3e-79	41.60	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	4.1e-78	41.60	Pectin lyase-like superfamily protein	[more]
AT2G26620.1	1.7e-76	42.49	Pectin lyase-like superfamily protein	[more]
AT1G17150.1	3.9e-76	41.88	Pectin lyase-like superfamily protein	[more]
AT1G43080.1	3.9e-76	41.41	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 126..152 e-value: 70.0 score: 12.3 coord: 176..196 e-value: 7500.0 score: 1.0 coord: 206..227 e-value: 3.3 score: 16.7 coord: 270..297 e-value: 4700.0 score: 2.4 coord: 153..174 e-value: 1300.0 score: 6.4
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 18..330 e-value: 1.1E-84 score: 284.3
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 183..196
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..340 e-value: 1.5E-99 score: 335.8
None	No IPR available	PANTHER	PTHR31375:SF124	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN	coord: 1..328
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..328
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 1..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G009310.1	CmaCh16G009310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity