CmaCh16G008220 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G008220
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	(+)-gamma-cadinene synthase-like
Location	Cma_Chr16: 4576944 .. 4577432 (+)
RNA-Seq Expression	CmaCh16G008220
Synteny	CmaCh16G008220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGCCCAAACACTCCTAAAGTCAAACGTCCTGTTGCAAAATATCATCCAAGCATATGGAAGGACCACTTCCTTTCACTAACATCTGATGCATCGGTAAATTAATCTTTAGTGTTCATGAATTATACCATAATCATAATTATTTCAAAATTTGTTTATTTTTTTGGACTTCCTTTTCAATTTTAGAATATAGATGTTGAGATGGAGGAACAAGCTAAAGAGTTGAAAGTAGGAGTGAAAAAGATGCTAATGATTTCTAATAGAAATCTTTTTGGGAAGTTACGTTTGGTTGATTCAATACAACGGTTAGGCGTATATTATCATTTCGAAAATGAGATTGATCAAGTCTTAGAAAAAATATATACAAACTATAGAGACTTCACAAGTATTGATGGTGACGAGGATCTTCACACTGTCTCTCTTCTATTTCGATTGCATAGACAACAAGGTTATAAAATTCCATGTGGTAAGTTTATTCATATATAA

mRNA sequence

ATGAAGAGCCCAAACACTCCTAAAGTCAAACGTCCTGTTGCAAAATATCATCCAAGCATATGGAAGGACCACTTCCTTTCACTAACATCTGATGCATCGAATATAGATGTTGAGATGGAGGAACAAGCTAAAGAGTTGAAAGTAGGAGTGAAAAAGATGCTAATGATTTCTAATAGAAATCTTTTTGGGAAGTTACGTTTGGTTGATTCAATACAACGGTTAGGCGTATATTATCATTTCGAAAATGAGATTGATCAAGTCTTAGAAAAAATATATACAAACTATAGAGACTTCACAAGTATTGATGGTGACGAGGATCTTCACACTGTCTCTCTTCTATTTCGATTGCATAGACAACAAGGTTATAAAATTCCATGTGGTAAGTTTATTCATATATAA

Coding sequence (CDS)

ATGAAGAGCCCAAACACTCCTAAAGTCAAACGTCCTGTTGCAAAATATCATCCAAGCATATGGAAGGACCACTTCCTTTCACTAACATCTGATGCATCGAATATAGATGTTGAGATGGAGGAACAAGCTAAAGAGTTGAAAGTAGGAGTGAAAAAGATGCTAATGATTTCTAATAGAAATCTTTTTGGGAAGTTACGTTTGGTTGATTCAATACAACGGTTAGGCGTATATTATCATTTCGAAAATGAGATTGATCAAGTCTTAGAAAAAATATATACAAACTATAGAGACTTCACAAGTATTGATGGTGACGAGGATCTTCACACTGTCTCTCTTCTATTTCGATTGCATAGACAACAAGGTTATAAAATTCCATGTGGTAAGTTTATTCATATATAA

Protein sequence

MKSPNTPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKLRLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIPCGKFIHI

Homology

[Close]

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 8.6e-29
Identity = 67/135 (49.63%), Postives = 90/135 (66.67%), Query Frame = 0


Query: 1   MKSPNTPKVKRPVAKYHPSIWKDHFLSLT-SDASNIDVEMEEQAKELKVGVKKMLMISNR 60
           MK+ + P VKR +A +HP+IWK+HFLS T  DA  ID  M+E+ ++LK  ++ M++    
Sbjct: 13  MKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLKEEIRMMMIAYVE 72

Query: 61  NLFGKLRLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSI----DGDEDLHTVSLLFR 120
           N   KL LVDSIQRLGV YHFE+E+D+ LE IY +Y +   +       EDLH  +LLFR
Sbjct: 73  NQLIKLNLVDSIQRLGVSYHFEDEVDEFLEHIYVSYNNSLLLSNKNSNGEDLHITALLFR 132

Query: 121 LHRQQGYKIPCGKFI 131
           L RQQGY+I C  F+
Sbjct: 133 LLRQQGYRISCDIFL 147

[Close]

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 4.0e-26
Identity = 59/124 (47.58%), Postives = 86/124 (69.35%), Query Frame = 0


Query: 6   TPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKL 65
           +P+V R  A +HPSIW DHFLS  S+ +N D  +++  ++LK  V+KMLM ++ +   KL
Sbjct: 17  SPEVGRRCANFHPSIWGDHFLSYASEFTNTDDHLKQHVQQLKEEVRKMLMAADDDSAQKL 76

Query: 66  RLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIP 125
            L+D+IQRLGV YHFE+EID+VL+ ++    D + +  +ED++T SL FRL RQQGY + 
Sbjct: 77  LLIDAIQRLGVAYHFESEIDEVLKHMF----DGSVVSAEEDVYTASLRFRLLRQQGYHVS 136

Query: 126 CGKF 130
           C  F
Sbjct: 137 CDLF 136

[Close]

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot
Match: B9S9Z3 (Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.4e-24
Identity = 58/124 (46.77%), Postives = 82/124 (66.13%), Query Frame = 0


Query: 6   TPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKL 65
           T  VKR  + YHP+IW DHFL+  S +  ID  +E+Q + LK  V+KM++  N     KL
Sbjct: 18  TSAVKRHSSTYHPTIWGDHFLANLSHSKIIDGSIEQQFEGLKQKVRKMIIDLNNEPCKKL 77

Query: 66  RLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIP 125
            L+D++QRLGV YHF++EI+ VL+K+Y +Y      D ++DL+TV+L FRL RQ G K+ 
Sbjct: 78  GLIDAVQRLGVGYHFKSEIEDVLQKVYHDYS-----DDEDDLNTVALRFRLLRQHGIKVS 136

Query: 126 CGKF 130
           C  F
Sbjct: 138 CAIF 136

[Close]

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot
Match: B9SBV6 (Probable terpene synthase 3 OS=Ricinus communis OX=3988 GN=TPS3 PE=3 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.6e-22
Identity = 54/127 (42.52%), Postives = 83/127 (65.35%), Query Frame = 0


Query: 3   SPNTPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLF 62
           +P   +V+R  A++HPS+W D+F+    D   + +  +EQAK L+  V++M++   +   
Sbjct: 8   APQRAQVRR-TAQFHPSVWGDYFIKNAPDNKMVSI-WKEQAKVLEEEVRRMIISETQKPS 67

Query: 63  GKLRLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGY 122
           GKL+L+D IQRLG+ YHFE EI +V+E++Y+NY      D DEDL+ ++L FRL RQQGY
Sbjct: 68  GKLKLIDVIQRLGIAYHFEEEIGEVIEQVYSNY------DDDEDLYDIALRFRLLRQQGY 126

Query: 123 KIPCGKF 130
            +    F
Sbjct: 128 NVSSDVF 126

[Close]

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot
Match: E3W207 (Sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 PE=2 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.1e-22
Identity = 53/127 (41.73%), Postives = 77/127 (60.63%), Query Frame = 0


Query: 4   PNTPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFG 63
           PN  ++ RP+A + PSIW D F++ T +  N   + E Q +ELK  V++ L  +      
Sbjct: 12  PNLKELSRPIANFPPSIWGDRFINYTCEDENEQAQKERQVEELKEQVRRELAATVDKPLQ 71

Query: 64  KLRLVDSIQRLGVYYHFENEIDQVLEKIYTN-YRDFTSIDGDEDLHTVSLLFRLHRQQGY 123
           +L ++D+ QRLG+ YHFENEI++ LE IY + Y       G  DL++V+L FRL RQ GY
Sbjct: 72  QLNIIDATQRLGIAYHFENEIEESLEHIYLHTYVKNNCFQGSHDLYSVALWFRLLRQDGY 131

Query: 124 KIPCGKF 130
           K+ C  F
Sbjct: 132 KVSCDVF 138

[Close]

BLAST of CmaCh16G008220 vs. TAIR 10
Match: AT3G14520.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 88.2 bits (217), Expect = 5.4e-18
Identity = 48/128 (37.50%), Postives = 74/128 (57.81%), Query Frame = 0


Query: 2   KSPNTPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNL 61
           KS +  +  RP   + PS+W DHFLS++ D    D E+E + + +K  VK MLM S  + 
Sbjct: 54  KSSDDLEGSRPSTYFSPSLWGDHFLSVSLDRGEFD-ELEREIETMKPLVKDMLMSSQSSD 113

Query: 62  FGKLRLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQG 121
             K+RL+  +  LG  YHF+ EI  +L+  +T   D   I G++DL T+S++F + R  G
Sbjct: 114 KEKIRLIHLLVSLGSSYHFDKEIQDILKHSFTKLDDI--IVGEDDLETISIMFEVFRLYG 173

Query: 122 YKIPCGKF 130
           +K+ C  F
Sbjct: 174 HKMSCDAF 178

[Close]

BLAST of CmaCh16G008220 vs. TAIR 10
Match: AT3G14540.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 3.5e-17
Identity = 48/132 (36.36%), Postives = 75/132 (56.82%), Query Frame = 0


Query: 2   KSPNTPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNL 61
           KS +  +  RP   + PS+W DHFLS++ D +   VE+E + + +K  VK MLM S  + 
Sbjct: 51  KSSDDLETSRPSTYFSPSLWGDHFLSVSLDHAEF-VELEREIETMKPLVKDMLMSSQSSD 110

Query: 62  FGKLRLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDG----DEDLHTVSLLFRLH 121
             K+RL+  +  LG  YHF+ EI  +L+        FT +DG    ++DL T+S++F + 
Sbjct: 111 KEKIRLIHLLVSLGSSYHFDKEIQDILK------HSFTKLDGIIVEEDDLETISIMFEVF 170

Query: 122 RQQGYKIPCGKF 130
           R  G+K+ C  F
Sbjct: 171 RLYGHKMSCDAF 175

[Close]

BLAST of CmaCh16G008220 vs. TAIR 10
Match: AT3G14490.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 2.3e-16
Identity = 43/119 (36.13%), Postives = 67/119 (56.30%), Query Frame = 0


Query: 11  RPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKLRLVDS 70
           RP+A + P++W DHFLS+    +  D    E    +K  V+ ML  S  +   ++RL+  
Sbjct: 60  RPLAHFSPTLWGDHFLSVPLHVAEFDDFSREIEVTMKPKVRDMLKSSKNSDNERIRLIHL 119

Query: 71  IQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIPCGKF 130
           +  LG+ YHFE EID++L + + N  D   I  + DL T+S +F + R +GY +PC  F
Sbjct: 120 LMNLGIAYHFEIEIDEILGQAFGNLDDI--IAKENDLETISTMFEVFRLRGYYMPCYAF 176

[Close]

BLAST of CmaCh16G008220 vs. TAIR 10
Match: AT3G29410.1 (Terpenoid cyclases/Protein prenyltransferases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 3.9e-16
Identity = 41/119 (34.45%), Postives = 69/119 (57.98%), Query Frame = 0


Query: 11  RPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKLRLVDS 70
           RP+  + PS W DHFLS++ D S  +   +E     K  V+ MLM  + +   ++RL+  
Sbjct: 64  RPLTYFSPSYWGDHFLSVSIDDSEFEALEKEIETVFKPKVRDMLMSPHSSDKERIRLIHL 123

Query: 71  IQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIPCGKF 130
           +  LG+ Y++ENEI+++L K Y        I  ++DL T++++F + R  G+K+PC  F
Sbjct: 124 LISLGIAYYYENEIEEILHKAYGKLA--CLISDEDDLETIAIMFEVFRLYGHKMPCDVF 180

[Close]

BLAST of CmaCh16G008220 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 78.6 bits (192), Expect = 4.3e-15
Identity = 50/134 (37.31%), Postives = 68/134 (50.75%), Query Frame = 0


Query: 6   TPKVKRPVAKYHPSIWKDHFLSLTSDASNIDVEMEEQAKELKVGVKKMLMISNRNLFGKL 65
           +P V+R  A Y PS W  H L    +    D  + E+   LK  V+KML    +    +L
Sbjct: 41  SPTVQRRSANYQPSRWDHHHLLSVENKFAKDKRVRER-DLLKEKVRKMLNDEQKTYLDQL 100

Query: 66  RLVDSIQRLGVYYHFENEIDQVLEKIYTNYRDFTSIDGDEDLHTVSLLFRLHRQQGYKIP 125
             +D +Q+LGV YHFE EID +L   Y   +D T+I  + DLH  +L FRL RQ G+ + 
Sbjct: 101 EFIDDLQKLGVSYHFEAEIDNILTSSYK--KDRTNIQ-ESDLHATALEFRLFRQHGFNVS 160

Query: 126 ----------CGKF 130
                     CGKF
Sbjct: 161 EDVFDVFMENCGKF 170

The following BLAST results are available for this feature:

BLAST of CmaCh16G008220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2KSJ5	8.6e-29	49.63	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q6Q3H3	4.0e-26	47.58	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
B9S9Z3	8.4e-24	46.77	Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1	[more]
B9SBV6	1.6e-22	42.52	Probable terpene synthase 3 OS=Ricinus communis OX=3988 GN=TPS3 PE=3 SV=1	[more]
E3W207	2.1e-22	41.73	Sesquiterpene synthase OS=Santalum austrocaledonicum OX=293154 PE=2 SV=1	[more]

BLAST of CmaCh16G008220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14520.1	5.4e-18	37.50	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14540.1	3.5e-17	36.36	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G14490.1	2.3e-16	36.13	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT3G29410.1	3.9e-16	34.45	Terpenoid cyclases/Protein prenyltransferases superfamily protein	[more]
AT2G24210.1	4.3e-15	37.31	terpene synthase 10	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036965	Terpene synthase, N-terminal domain superfamily	GENE3D	1.50.10.130		coord: 59..129 e-value: 9.3E-31 score: 108.7
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 18..58 e-value: 9.3E-31 score: 108.7
IPR001906	Terpene synthase, N-terminal domain	PFAM	PF01397	Terpene_synth	coord: 20..129 e-value: 2.0E-29 score: 102.6
None	No IPR available	PANTHER	PTHR31225	OS04G0344100 PROTEIN-RELATED	coord: 7..129
None	No IPR available	PANTHER	PTHR31225:SF130	TERPENE SYNTHASE FAMILY, METAL-BINDING DOMAIN PROTEIN	coord: 7..129
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 17..129

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G008220.1	CmaCh16G008220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0045338	farnesyl diphosphate metabolic process
biological_process	GO:0051762	sesquiterpene biosynthetic process
molecular_function	GO:0000287	magnesium ion binding
molecular_function	GO:0010334	sesquiterpene synthase activity
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0010333	terpene synthase activity