CmaCh16G006230 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G006230 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 63.9 bits (154), Expect = 3.3e-09
Identity = 32/102 (31.37%), Postives = 58/102 (56.86%), Query Frame = 0

Query: 60  KPVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAA 119
           KP+++L+HGF  E   +W+ Q+      Y V   DL  +G+S  D      +++ +CL A
Sbjct: 91  KPLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGES--DAPAHQESYKLDCLIA 150

Query: 120 GLR----KLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVV 158
            ++     LG  KC ++G  +GGM+A+ +A  +PE++  ++V
Sbjct: 151 DIKDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIV 189

BLAST of CmaCh16G006230 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 4.3e-09
Identity = 32/102 (31.37%), Postives = 57/102 (55.88%), Query Frame = 0

Query: 60  KPVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAA 119
           KP+++L+HGF  E   +W++Q+      Y V   DL  +G+  TD       ++ +CL  
Sbjct: 93  KPLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDLRGYGE--TDAPIHRQNYKLDCLIT 152

Query: 120 GLR----KLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVV 158
            ++     LG  KC ++G  +GGM+A+ +A  +PE+V  ++V
Sbjct: 153 DIKDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIV 191

BLAST of CmaCh16G006230 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.6e-08
Identity = 40/126 (31.75%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 61  PVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAG 120
           P ++L+HG   +   TW      L++R++V  PDLL  G S   ++D S    A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 121 LRKLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGS 180
           L  L I++ T+VG S GG VA + A  +P+LV+ +++  +     D      L  L  GS
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 181 SSELLL 187
            +  LL
Sbjct: 158 EAMALL 162

BLAST of CmaCh16G006230 vs. ExPASy Swiss-Prot
Match: P9WNH3 (Uncharacterized protein Rv2715 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2715 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.6e-08
Identity = 40/126 (31.75%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 61  PVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAG 120
           P ++L+HG   +   TW      L++R++V  PDLL  G S   ++D S    A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 121 LRKLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGS 180
           L  L I++ T+VG S GG VA + A  +P+LV+ +++  +     D      L  L  GS
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 181 SSELLL 187
            +  LL
Sbjct: 158 EAMALL 162

BLAST of CmaCh16G006230 vs. ExPASy Swiss-Prot
Match: P0A573 (Uncharacterized protein Mb2734 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2734 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.6e-08
Identity = 40/126 (31.75%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 61  PVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAG 120
           P ++L+HG   +   TW      L++R++V  PDLL  G S   ++D S    A  +   
Sbjct: 38  PAILLIHGI-GDNSTTWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 121 LRKLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGS 180
           L  L I++ T+VG S GG VA + A  +P+LV+ +++  +     D      L  L  GS
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAGGVTKDVNIVFRLASLPMGS 157

Query: 181 SSELLL 187
            +  LL
Sbjct: 158 EAMALL 162

BLAST of CmaCh16G006230 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 9.2e-108
Identity = 192/314 (61.15%), Postives = 233/314 (74.20%), Query Frame = 0

Query: 1   MVNLVAAQRPLLHALMKMAGVVPYSVEFEPGTTMNFWVPVETLSKPKKTQPPKPPQKPTK 60
           MVN V  Q+PLL+ LMKMAGVVPY++E EPGT +NFWVP ETL K   T  P  P KP K
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 61  PVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAG 120
           PVV+L+HGFA EGIVTWQFQVGALSK+YSVY+PDLLFFG S TD SDRSP FQA+CL  G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 121 LRKLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGS 180
           LR LG+DK   VGFSYGGMVAFK+AE +P++V A+VVSGSI  MTD+I++ +L RLGF S
Sbjct: 121 LRILGVDKFVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFSS 180

Query: 181 SSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLE----------------------- 240
           S++LLLPTSV GLKAL ++A HK LWFP RL KD++E                       
Sbjct: 181 STDLLLPTSVTGLKALFTIAVHKPLWFPKRLFKDYIEVMFNNRKERAELLEAVVVSNKEA 240

Query: 241 -------KIHLLWGLNDQIFNVELARKLQEELGDNATFQSIPKSGHLVHLERPCIYNRCL 285
                  KIH LWG +DQIF++ELAR ++E++G+NAT +SI K+GHLV LERPC+YNR L
Sbjct: 241 QIPHFPRKIHFLWGESDQIFDLELARDMKEQIGENATIESIKKAGHLVQLERPCVYNRRL 300

BLAST of CmaCh16G006230 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 385.6 bits (989), Expect = 3.5e-107
Identity = 191/311 (61.41%), Postives = 236/311 (75.88%), Query Frame = 0

Query: 1   MVNLVAAQRPLLHALMKMAGVVPYSVEFEPGTTMNFWVPVETLSKPKKTQP--PKPPQKP 60
           MVN V  Q+PLL+ LMK+AGV+PY+VE EPGT MNFW+P ETL K KK+       PQKP
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 61  TKPVVVLVHGFAAEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLA 120
           TKPV++ +HGFAAEGIVTWQFQVG+L+K+YSVY+PDLLFFG S +D +DRSP FQA CL 
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 121 AGLRKLGIDKCTVVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGF 180
             LR LGI+K T+VGFSYGGMVAFK+AE +PE+V+AMVVSGSILAMTD+IS+  L +LGF
Sbjct: 121 KSLRILGIEKFTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLGF 180

Query: 181 GSSSELLLPTSVKGLKALLSVAAHKKLWFPDRLHKDFLE--------------------- 240
            SS++LLLPTSVKGLK L ++A HK +WFP RL KDF+E                     
Sbjct: 181 KSSADLLLPTSVKGLKTLFTLAVHKPMWFPKRLFKDFIEVMITNRKERAELLEALVISNK 240

Query: 241 ---------KIHLLWGLNDQIFNVELARKLQEELGDNATFQSIPKSGHLVHLERPCIYNR 280
                    KIHLLWG +DQIFN+E A+ ++E+LG+NAT +SI K+GHL HLERPC+YNR
Sbjct: 241 DVTIPRFQQKIHLLWGESDQIFNLEFAKSMKEQLGENATMESIKKAGHLAHLERPCVYNR 300

BLAST of CmaCh16G006230 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.2e-36
Identity = 94/298 (31.54%), Postives = 150/298 (50.34%), Query Frame = 0

Query: 12  LHALMKMAGVVPYSVEFEPGTTMNFWVPVETLSKPKKTQPPKPPQKPTKPVVVLVHGFAA 71
           L   ++ AG+   ++  +  TT++FW P           P        +PV++L+HGF  
Sbjct: 17  LRRCLRAAGLTSQTLSIDSETTIHFWGP----------PPLDHRSDDDRPVMLLLHGFGP 76

Query: 72  EGIVTWQFQVGALS-KRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAGLRKLGIDKCT 131
             +  W+ Q+ A S   + VY PDL+FFGDS +  ++R+  FQAEC+A  + K+GI K  
Sbjct: 77  SSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGKYN 136

Query: 132 VVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGSSSELLLPTSV 191
           V G SYGG VA+ +A++WPE VE +V++ S + M     +  LQR       +++LP++ 
Sbjct: 137 VAGTSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVMLPSTA 196

Query: 192 KGLKALLSVAAHKKL--WFPDRLHKDFL---------EKIHLL----------------- 251
              + L+++A+  +L   FPD L  D +         EKI LL                 
Sbjct: 197 TEFRTLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDSLS 256

Query: 252 ------WGLNDQIFNVELARKLQEELGDNATFQSIPKSGHLVHLERPCIYNRCLKQFL 275
                 WG  DQIF V++A +L+E LGD    + I  + H+  +E    +N  + +FL
Sbjct: 257 QEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

BLAST of CmaCh16G006230 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 142.5 bits (358), Expect = 5.2e-34
Identity = 88/297 (29.63%), Postives = 145/297 (48.82%), Query Frame = 0

Query: 11  LLHALMKMAGVVPYSVEFEPGTTMNFWVPVETLSKPKKTQPPKPPQKPTKPVVVLVHGFA 70
           LL      AG+   ++  +  TT+ FW P           P    +   KP ++L+HGF 
Sbjct: 15  LLRRRFSSAGLSLQTLSIDSETTIQFWGP----------PPSSSSENTQKPSLLLLHGFG 74

Query: 71  AEGIVTWQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAGLRKLGIDKCT 130
              +  W  QV  LS  + +YVPDL+FFG S +   +RS  FQA C+   + KL +++ +
Sbjct: 75  PSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFS 134

Query: 131 VVGFSYGGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLGFGSSSELLLPTSV 190
           VVG SYGG VA+ +A+++PE VE +V++ S + +  S ++  + R       E++LP S 
Sbjct: 135 VVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPASA 194

Query: 191 KGLKALLSVAAHKKL-WFPDRLHKDFLEKIH----------------------------- 250
             L+    + + K+L + PD +  DF +K++                             
Sbjct: 195 TDLRRFSGMVSSKRLDYVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNVSPIQQ 254

Query: 251 ---LLWGLNDQIFNVELARKLQEELGDNATFQSIPKSGHLVHLERPCIYNRCLKQFL 275
              L+WG  DQ+F +++A  L+E LG  AT + I K+ H+   E+   +N  +  FL
Sbjct: 255 DVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMSFL 301

BLAST of CmaCh16G006230 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 8.8e-34
Identity = 84/294 (28.57%), Postives = 148/294 (50.34%), Query Frame = 0

Query: 17  KMAGVVPYSVEFEPGTTMNFWVPVETLSKPKKTQPPKPPQKPTKPVVVLVHGFAAEGIVT 76
           K +G+ P +++ + GT +NFWV         KT+P   P    KP ++L+HG  A  I  
Sbjct: 20  KRSGLRPVTIDLKDGTVVNFWV--------SKTKPESKP----KPNLLLIHGLGATAIWQ 79

Query: 77  WQFQVGALSKRYSVYVPDLLFFGDSITDKSDRSPTFQAECLAAGLRKLGIDKCTVVGFSY 136
           W      LS+ +++Y+PDL+FFG S T + +RS  FQA+ L   L    + K ++VG SY
Sbjct: 80  WYDVARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSY 139

Query: 137 GGMVAFKLAELWPELVEAMVVSGSILAMTDSISDETLQRLG-FGSSSELLLPTSVKGLKA 196
           GG V +++A ++ + VE +V+  + + + +      + ++     +S++L+P SVK L+ 
Sbjct: 140 GGFVGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRE 199

Query: 197 LLSVAAHKKL---WFPDRLHKDFLE--------------------------------KIH 256
           L+    +K       P  L  DF+E                                   
Sbjct: 200 LMGYIFYKPALARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIISEIPKLKQPTL 259

Query: 257 LLWGLNDQIFNVELARKLQEELGDNATFQSIPKSGHLVHLERPCIYNRCLKQFL 275
           ++WG +DQ+F +E+ ++L++ +GDN     I ++GH+ + E+P  + + LK FL
Sbjct: 260 IIWGEHDQVFPLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFL 301