CmaCh16G006110 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G006110
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	GDP-mannose 4,6-dehydratase
Location	Cma_Chr16: 3174427 .. 3175533 (-)
RNA-Seq Expression	CmaCh16G006110
Synteny	CmaCh16G006110

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCTATCGCCGACGCCACCAGATCCTCTTCCGACACTAACGGCGTACCGGCGGAGAAGCGCCGGACGGCCTTAATTACCGGCATTACTGGCCAGGATGGTTCGTACCTGACCGAGTTTCTTCTCGAGAAGGGCTATGAAGTCCATGGATTGATCCGGCGATCTTCCAACTTCAACACTCAGCGAATCAACCACATCTACATCGACCCTCACAATGTTCACAAGGCACGAATGAAGCTTCACTATGCTGACCTCACCGACGCCTCCTCTCTCCGCCGATGGCTCGATATCATCCAACCCGATGAGGTCTATAACCTCGCTGCTCAATCGCATGTTGCTGTTTCTTTTGAAATTCCGGATTATACTGCCGATGTTGTCGCCACTGGTGCCCTCCGTCTGCTTGAGGCCGTTCGATCTCATATCTCTGCCACTGGCCGTACTCAAATTCGGTACTACCAAGCGGGATCGTCTGAAATGTTCGGGTCTACTCCGCCGCCTCAGTCGGAGACGACTCCATTTCATCCTCGATCCCCTTACGCTGCAGCGAAGTGCGCCGCGCATTGGTATACTGTGAATTACCGTGAGGCCTACGGGATCTTCGCTTGTAATGGAATACTATTCAACCACGAATCGCCGCGCAGGGGAGAGAATTTCGTGACAAGGAAAATCACACGGGCTGTAGGGCGCATCAAGATTGGGCTGCAAAGCAAGTTGTTCTTGGGGAATCTGCAGGCGTCGAGGGATTGGGGTTTTGCAGGGGACTACGTGGAAGCCATGTGGCTAATGCTACAGCAAGAACAACCCGATGATTACGTGGTTGCGACGGAGGAATCACACACAGTGGAGGAGTTTTTGGAGGTCGCATTTGGGAATGTCGGATTGAATTGGAAGGACCATGTGGTGATCGACAAGAGATACTTCCGGCCGGCGGAGGTCGACAATCTCAAGGGGGATGCAAGCAAGGCGAGGAAGGTATTGGGATGGAAACCAAAGGTTGGATTTGAGAAGCTGGTGAAGATGATGGTGGATGAAGACATTGAAATAGCCAAGCGGGAGAAGGTGTTGGTCGATGCTGGCTACATGGATGCCCAGCAGCAACCTTGA

mRNA sequence

ATGGCATCTATCGCCGACGCCACCAGATCCTCTTCCGACACTAACGGCGTACCGGCGGAGAAGCGCCGGACGGCCTTAATTACCGGCATTACTGGCCAGGATGGTTCGTACCTGACCGAGTTTCTTCTCGAGAAGGGCTATGAAGTCCATGGATTGATCCGGCGATCTTCCAACTTCAACACTCAGCGAATCAACCACATCTACATCGACCCTCACAATGTTCACAAGGCACGAATGAAGCTTCACTATGCTGACCTCACCGACGCCTCCTCTCTCCGCCGATGGCTCGATATCATCCAACCCGATGAGGTCTATAACCTCGCTGCTCAATCGCATGTTGCTGTTTCTTTTGAAATTCCGGATTATACTGCCGATGTTGTCGCCACTGGTGCCCTCCGTCTGCTTGAGGCCGTTCGATCTCATATCTCTGCCACTGGCCGTACTCAAATTCGGTACTACCAAGCGGGATCGTCTGAAATGTTCGGGTCTACTCCGCCGCCTCAGTCGGAGACGACTCCATTTCATCCTCGATCCCCTTACGCTGCAGCGAAGTGCGCCGCGCATTGGTATACTGTGAATTACCGTGAGGCCTACGGGATCTTCGCTTGTAATGGAATACTATTCAACCACGAATCGCCGCGCAGGGGAGAGAATTTCGTGACAAGGAAAATCACACGGGCTGTAGGGCGCATCAAGATTGGGCTGCAAAGCAAGTTGTTCTTGGGGAATCTGCAGGCGTCGAGGGATTGGGGTTTTGCAGGGGACTACGTGGAAGCCATGTGGCTAATGCTACAGCAAGAACAACCCGATGATTACGTGGTTGCGACGGAGGAATCACACACAGTGGAGGAGTTTTTGGAGGTCGCATTTGGGAATGTCGGATTGAATTGGAAGGACCATGTGGTGATCGACAAGAGATACTTCCGGCCGGCGGAGGTCGACAATCTCAAGGGGGATGCAAGCAAGGCGAGGAAGGTATTGGGATGGAAACCAAAGGTTGGATTTGAGAAGCTGGTGAAGATGATGGTGGATGAAGACATTGAAATAGCCAAGCGGGAGAAGGTGTTGGTCGATGCTGGCTACATGGATGCCCAGCAGCAACCTTGA

Coding sequence (CDS)

ATGGCATCTATCGCCGACGCCACCAGATCCTCTTCCGACACTAACGGCGTACCGGCGGAGAAGCGCCGGACGGCCTTAATTACCGGCATTACTGGCCAGGATGGTTCGTACCTGACCGAGTTTCTTCTCGAGAAGGGCTATGAAGTCCATGGATTGATCCGGCGATCTTCCAACTTCAACACTCAGCGAATCAACCACATCTACATCGACCCTCACAATGTTCACAAGGCACGAATGAAGCTTCACTATGCTGACCTCACCGACGCCTCCTCTCTCCGCCGATGGCTCGATATCATCCAACCCGATGAGGTCTATAACCTCGCTGCTCAATCGCATGTTGCTGTTTCTTTTGAAATTCCGGATTATACTGCCGATGTTGTCGCCACTGGTGCCCTCCGTCTGCTTGAGGCCGTTCGATCTCATATCTCTGCCACTGGCCGTACTCAAATTCGGTACTACCAAGCGGGATCGTCTGAAATGTTCGGGTCTACTCCGCCGCCTCAGTCGGAGACGACTCCATTTCATCCTCGATCCCCTTACGCTGCAGCGAAGTGCGCCGCGCATTGGTATACTGTGAATTACCGTGAGGCCTACGGGATCTTCGCTTGTAATGGAATACTATTCAACCACGAATCGCCGCGCAGGGGAGAGAATTTCGTGACAAGGAAAATCACACGGGCTGTAGGGCGCATCAAGATTGGGCTGCAAAGCAAGTTGTTCTTGGGGAATCTGCAGGCGTCGAGGGATTGGGGTTTTGCAGGGGACTACGTGGAAGCCATGTGGCTAATGCTACAGCAAGAACAACCCGATGATTACGTGGTTGCGACGGAGGAATCACACACAGTGGAGGAGTTTTTGGAGGTCGCATTTGGGAATGTCGGATTGAATTGGAAGGACCATGTGGTGATCGACAAGAGATACTTCCGGCCGGCGGAGGTCGACAATCTCAAGGGGGATGCAAGCAAGGCGAGGAAGGTATTGGGATGGAAACCAAAGGTTGGATTTGAGAAGCTGGTGAAGATGATGGTGGATGAAGACATTGAAATAGCCAAGCGGGAGAAGGTGTTGGTCGATGCTGGCTACATGGATGCCCAGCAGCAACCTTGA

Protein sequence

MASIADATRSSSDTNGVPAEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGSTPPPQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDHVVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREKVLVDAGYMDAQQQP

Homology

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BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot
Match: P93031 (GDP-mannose 4,6 dehydratase 2 OS=Arabidopsis thaliana OX=3702 GN=MUR1 PE=1 SV=3)

HSP 1 Score: 649.0 bits (1673), Expect = 3.1e-185
Identity = 319/374 (85.29%), Postives = 349/374 (93.32%), Query Frame = 0


Query: 1   MASIADATRSSSDTNGVP------AEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIR 60
           MAS  + +RS S++   P       E R+ ALITGITGQDGSYLTEFLL KGYEVHGLIR
Sbjct: 1   MASENNGSRSDSESITAPKADSTVVEPRKIALITGITGQDGSYLTEFLLGKGYEVHGLIR 60

Query: 61  RSSNFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAAQSHVA 120
           RSSNFNTQRINHIYIDPHNV+KA MKLHYADLTDASSLRRW+D+I+PDEVYNLAAQSHVA
Sbjct: 61  RSSNFNTQRINHIYIDPHNVNKALMKLHYADLTDASSLRRWIDVIKPDEVYNLAAQSHVA 120

Query: 121 VSFEIPDYTADVVATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGSTPPPQSETTPF 180
           VSFEIPDYTADVVATGALRLLEAVRSH   +GRT ++YYQAGSSEMFGSTPPPQSETTPF
Sbjct: 121 VSFEIPDYTADVVATGALRLLEAVRSHTIDSGRT-VKYYQAGSSEMFGSTPPPQSETTPF 180

Query: 181 HPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIG 240
           HPRSPYAA+KCAAHWYTVNYREAYG+FACNGILFNHESPRRGENFVTRKITRA+GRIK+G
Sbjct: 181 HPRSPYAASKCAAHWYTVNYREAYGLFACNGILFNHESPRRGENFVTRKITRALGRIKVG 240

Query: 241 LQSKLFLGNLQASRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVG 300
           LQ+KLFLGNLQASRDWGFAGDYVEAMWLMLQQE+PDDYVVATEE HTVEEFL+V+FG +G
Sbjct: 241 LQTKLFLGNLQASRDWGFAGDYVEAMWLMLQQEKPDDYVVATEEGHTVEEFLDVSFGYLG 300

Query: 301 LNWKDHVVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREK 360
           LNWKD+V ID+RYFRPAEVDNL+GDASKA++VLGWKP+VGFEKLVKMMVDED+E+AKREK
Sbjct: 301 LNWKDYVEIDQRYFRPAEVDNLQGDASKAKEVLGWKPQVGFEKLVKMMVDEDLELAKREK 360

Query: 361 VLVDAGYMDAQQQP 369
           VLVDAGYMDA+QQP
Sbjct: 361 VLVDAGYMDAKQQP 373

[Close]

BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot
Match: Q9SNY3 (GDP-mannose 4,6 dehydratase 1 OS=Arabidopsis thaliana OX=3702 GN=GMD1 PE=1 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.0e-183
Identity = 312/362 (86.19%), Postives = 342/362 (94.48%), Query Frame = 0


Query: 7   ATRSSSDTNGVPAEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINH 66
           A+RS +  + +  + R+ AL+TGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQR+NH
Sbjct: 2   ASRSLNGDSDI-VKPRKIALVTGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRLNH 61

Query: 67  IYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADV 126
           IY+DPHNV+KA MKLHY DL+DASSLRRWLD+I+PDEVYNLAAQSHVAVSFEIPDYTADV
Sbjct: 62  IYVDPHNVNKALMKLHYGDLSDASSLRRWLDVIKPDEVYNLAAQSHVAVSFEIPDYTADV 121

Query: 127 VATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGSTPPPQSETTPFHPRSPYAAAKCA 186
           VATGALRLLEAVRSH    GR  I+YYQAGSSEMFGSTPPPQSETTPFHPRSPYAA+KCA
Sbjct: 122 VATGALRLLEAVRSHNIDNGRA-IKYYQAGSSEMFGSTPPPQSETTPFHPRSPYAASKCA 181

Query: 187 AHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQA 246
           AHWYTVNYREAYG++ACNGILFNHESPRRGENFVTRKITRA+GRIK+GLQ+KLFLGN+QA
Sbjct: 182 AHWYTVNYREAYGLYACNGILFNHESPRRGENFVTRKITRALGRIKVGLQTKLFLGNIQA 241

Query: 247 SRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDHVVIDKR 306
           SRDWGFAGDYVEAMWLMLQQE+PDDYVVATEESHTV+EFL+V+FG VGLNWKDHV IDKR
Sbjct: 242 SRDWGFAGDYVEAMWLMLQQEKPDDYVVATEESHTVKEFLDVSFGYVGLNWKDHVEIDKR 301

Query: 307 YFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREKVLVDAGYMDAQQ 366
           YFRP EVDNLKGDASKA+++LGWKPKVGFEKLVKMMVDED+E+AKREKVL DAGYMDAQQ
Sbjct: 302 YFRPTEVDNLKGDASKAKEMLGWKPKVGFEKLVKMMVDEDLELAKREKVLADAGYMDAQQ 361

Query: 367 QP 369
           QP
Sbjct: 362 QP 361

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BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot
Match: P0AC90 (GDP-mannose 4,6-dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=gmd PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.1e-108
Identity = 200/367 (54.50%), Postives = 256/367 (69.75%), Query Frame = 0


Query: 23  RTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINHIYIDPHNVHKARMKLH 82
           + ALITG+TGQDGSYL EFLLEKGYEVHG+ RR+S+FNT+R++HIY DPH  +  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 83  YADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 142
           Y DL+D S+L R L  +QPDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R  +
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR-FL 122

Query: 143 SATGRTQIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 202
               +T  R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 GLEKKT--RFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 203 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 262
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 263 LMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDH--------------------- 322
           +MLQQEQP+D+V+AT   ++V +F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 323 ------VVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREK 362
                 + +D RYFRPAEV+ L GD +KA + LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

[Close]

BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot
Match: P0AC89 (GDP-mannose 4,6-dehydratase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=gmd PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.1e-108
Identity = 200/367 (54.50%), Postives = 256/367 (69.75%), Query Frame = 0


Query: 23  RTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINHIYIDPHNVHKARMKLH 82
           + ALITG+TGQDGSYL EFLLEKGYEVHG+ RR+S+FNT+R++HIY DPH  +  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 83  YADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 142
           Y DL+D S+L R L  +QPDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R  +
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR-FL 122

Query: 143 SATGRTQIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 202
               +T  R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 GLEKKT--RFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 203 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 262
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 263 LMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDH--------------------- 322
           +MLQQEQP+D+V+AT   ++V +F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 323 ------VVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREK 362
                 + +D RYFRPAEV+ L GD +KA + LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

[Close]

BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot
Match: P0AC88 (GDP-mannose 4,6-dehydratase OS=Escherichia coli (strain K12) OX=83333 GN=gmd PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 3.1e-108
Identity = 200/367 (54.50%), Postives = 256/367 (69.75%), Query Frame = 0


Query: 23  RTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINHIYIDPHNVHKARMKLH 82
           + ALITG+TGQDGSYL EFLLEKGYEVHG+ RR+S+FNT+R++HIY DPH  +  +  LH
Sbjct: 3   KVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCN-PKFHLH 62

Query: 83  YADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADVVATGALRLLEAVRSHI 142
           Y DL+D S+L R L  +QPDEVYNL A SHVAVSFE P+YTADV A G LRLLEA+R  +
Sbjct: 63  YGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIR-FL 122

Query: 143 SATGRTQIRYYQAGSSEMFGSTPP-PQSETTPFHPRSPYAAAKCAAHWYTVNYREAYGIF 202
               +T  R+YQA +SE++G     PQ ETTPF+PRSPYA AK  A+W TVNYRE+YG++
Sbjct: 123 GLEKKT--RFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMY 182

Query: 203 ACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMW 262
           ACNGILFNHESPRRGE FVTRKITRA+  I  GL+S L+LGN+ + RDWG A DYV+  W
Sbjct: 183 ACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQW 242

Query: 263 LMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDH--------------------- 322
           +MLQQEQP+D+V+AT   ++V +F+E+A   +G+  +                       
Sbjct: 243 MMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGV 302

Query: 323 ------VVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREK 362
                 + +D RYFRPAEV+ L GD +KA + LGWKP++   ++V  MV  D+E AK+  
Sbjct: 303 KPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHS 362

[Close]

BLAST of CmaCh16G006110 vs. TAIR 10
Match: AT3G51160.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 2.2e-186
Identity = 319/374 (85.29%), Postives = 349/374 (93.32%), Query Frame = 0


Query: 1   MASIADATRSSSDTNGVP------AEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIR 60
           MAS  + +RS S++   P       E R+ ALITGITGQDGSYLTEFLL KGYEVHGLIR
Sbjct: 1   MASENNGSRSDSESITAPKADSTVVEPRKIALITGITGQDGSYLTEFLLGKGYEVHGLIR 60

Query: 61  RSSNFNTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAAQSHVA 120
           RSSNFNTQRINHIYIDPHNV+KA MKLHYADLTDASSLRRW+D+I+PDEVYNLAAQSHVA
Sbjct: 61  RSSNFNTQRINHIYIDPHNVNKALMKLHYADLTDASSLRRWIDVIKPDEVYNLAAQSHVA 120

Query: 121 VSFEIPDYTADVVATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGSTPPPQSETTPF 180
           VSFEIPDYTADVVATGALRLLEAVRSH   +GRT ++YYQAGSSEMFGSTPPPQSETTPF
Sbjct: 121 VSFEIPDYTADVVATGALRLLEAVRSHTIDSGRT-VKYYQAGSSEMFGSTPPPQSETTPF 180

Query: 181 HPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIG 240
           HPRSPYAA+KCAAHWYTVNYREAYG+FACNGILFNHESPRRGENFVTRKITRA+GRIK+G
Sbjct: 181 HPRSPYAASKCAAHWYTVNYREAYGLFACNGILFNHESPRRGENFVTRKITRALGRIKVG 240

Query: 241 LQSKLFLGNLQASRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVG 300
           LQ+KLFLGNLQASRDWGFAGDYVEAMWLMLQQE+PDDYVVATEE HTVEEFL+V+FG +G
Sbjct: 241 LQTKLFLGNLQASRDWGFAGDYVEAMWLMLQQEKPDDYVVATEEGHTVEEFLDVSFGYLG 300

Query: 301 LNWKDHVVIDKRYFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREK 360
           LNWKD+V ID+RYFRPAEVDNL+GDASKA++VLGWKP+VGFEKLVKMMVDED+E+AKREK
Sbjct: 301 LNWKDYVEIDQRYFRPAEVDNLQGDASKAKEVLGWKPQVGFEKLVKMMVDEDLELAKREK 360

Query: 361 VLVDAGYMDAQQQP 369
           VLVDAGYMDA+QQP
Sbjct: 361 VLVDAGYMDAKQQP 373

[Close]

BLAST of CmaCh16G006110 vs. TAIR 10
Match: AT5G66280.1 (GDP-D-mannose 4,6-dehydratase 1 )

HSP 1 Score: 644.0 bits (1660), Expect = 7.1e-185
Identity = 312/362 (86.19%), Postives = 342/362 (94.48%), Query Frame = 0


Query: 7   ATRSSSDTNGVPAEKRRTALITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINH 66
           A+RS +  + +  + R+ AL+TGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQR+NH
Sbjct: 2   ASRSLNGDSDI-VKPRKIALVTGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRLNH 61

Query: 67  IYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAAQSHVAVSFEIPDYTADV 126
           IY+DPHNV+KA MKLHY DL+DASSLRRWLD+I+PDEVYNLAAQSHVAVSFEIPDYTADV
Sbjct: 62  IYVDPHNVNKALMKLHYGDLSDASSLRRWLDVIKPDEVYNLAAQSHVAVSFEIPDYTADV 121

Query: 127 VATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGSTPPPQSETTPFHPRSPYAAAKCA 186
           VATGALRLLEAVRSH    GR  I+YYQAGSSEMFGSTPPPQSETTPFHPRSPYAA+KCA
Sbjct: 122 VATGALRLLEAVRSHNIDNGRA-IKYYQAGSSEMFGSTPPPQSETTPFHPRSPYAASKCA 181

Query: 187 AHWYTVNYREAYGIFACNGILFNHESPRRGENFVTRKITRAVGRIKIGLQSKLFLGNLQA 246
           AHWYTVNYREAYG++ACNGILFNHESPRRGENFVTRKITRA+GRIK+GLQ+KLFLGN+QA
Sbjct: 182 AHWYTVNYREAYGLYACNGILFNHESPRRGENFVTRKITRALGRIKVGLQTKLFLGNIQA 241

Query: 247 SRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDHVVIDKR 306
           SRDWGFAGDYVEAMWLMLQQE+PDDYVVATEESHTV+EFL+V+FG VGLNWKDHV IDKR
Sbjct: 242 SRDWGFAGDYVEAMWLMLQQEKPDDYVVATEESHTVKEFLDVSFGYVGLNWKDHVEIDKR 301

Query: 307 YFRPAEVDNLKGDASKARKVLGWKPKVGFEKLVKMMVDEDIEIAKREKVLVDAGYMDAQQ 366
           YFRP EVDNLKGDASKA+++LGWKPKVGFEKLVKMMVDED+E+AKREKVL DAGYMDAQQ
Sbjct: 302 YFRPTEVDNLKGDASKAKEMLGWKPKVGFEKLVKMMVDEDLELAKREKVLADAGYMDAQQ 361

Query: 367 QP 369
           QP
Sbjct: 362 QP 361

[Close]

BLAST of CmaCh16G006110 vs. TAIR 10
Match: AT2G47650.1 (UDP-xylose synthase 4 )

HSP 1 Score: 62.0 bits (149), Expect = 1.2e-09
Identity = 86/363 (23.69%), Postives = 146/363 (40.22%), Query Frame = 0


Query: 3   SIADATRSSSDTNGVPAEKRRTAL---ITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNF 62
           SI   +R  S    +P   +R  L   +TG  G  GS+L + L+ +G  V  +     NF
Sbjct: 98  SIEYMSRIGSAGGKIPLGLKRKVLRVVVTGGAGFVGSHLVDRLMARGDNVIVV----DNF 157

Query: 63  NTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAA-QSHVAVSFE 122
            T R  ++    H+ +    ++   D+ +         +++ D++Y+LA   S V   F 
Sbjct: 158 FTGRKENVM---HHFNNPNFEMIRHDVVEPI-------LLEVDQIYHLACPASPVHYKFN 217

Query: 123 IPDYTADVVATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGS-TPPPQSET-----T 182
            P  T      G L +L   +       R   R+    +SE++G     PQ ET      
Sbjct: 218 -PVKTIKTNVVGTLNMLGLAK-------RVGARFLLTSTSEVYGDPLQHPQVETYWGNVN 277

Query: 183 PFHPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRR-------GENFVTRKIT 242
           P   RS Y   K  A   T++Y     +      +FN   PR          NFV + + 
Sbjct: 278 PIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTYGPRMCIDDGRVVSNFVAQALR 337

Query: 243 RAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEF 302
           +         +     G+ + +R + F  D VE +  +++ E    + +      T+ E 
Sbjct: 338 K---------EPLTVYGDGKQTRSFQFVSDLVEGLMRLMEGEHVGPFNLGNPGEFTMLEL 397

Query: 303 LEVAFGNVGLNWKDHVVIDKRYFRPAEVDN---LKGDASKARKVLGWKPKVGFEKLVKMM 346
            +V    +  N K         FRP   D+    K D +KA+++LGW+PKV   + + +M
Sbjct: 398 AKVVQETIDPNAKIE-------FRPNTEDDPHKRKPDITKAKELLGWEPKVALRQGLPLM 422

[Close]

BLAST of CmaCh16G006110 vs. TAIR 10
Match: AT2G47650.2 (UDP-xylose synthase 4 )

HSP 1 Score: 60.1 bits (144), Expect = 4.4e-09
Identity = 88/365 (24.11%), Postives = 148/365 (40.55%), Query Frame = 0


Query: 3   SIADATRSSSDTNGVPAEKRRTAL---ITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNF 62
           SI   +R  S    +P   +R  L   +TG  G  GS+L + L+ +G  V  +     NF
Sbjct: 98  SIEYMSRIGSAGGKIPLGLKRKVLRVVVTGGAGFVGSHLVDRLMARGDNVIVV----DNF 157

Query: 63  NTQRINHIYIDPHNVHKARMKLHYADLTDASSLRRWLDIIQPDEVYNLAA-QSHVAVSFE 122
            T R  ++    H+ +    ++   D+ +         +++ D++Y+LA   S V   F 
Sbjct: 158 FTGRKENVM---HHFNNPNFEMIRHDVVEPI-------LLEVDQIYHLACPASPVHYKFN 217

Query: 123 IPDYTADVVATGALRLLEAVRSHISATGRTQIRYYQAGSSEMFGS-TPPPQSET-----T 182
            P  T      G L +L   +       R   R+    +SE++G     PQ ET      
Sbjct: 218 -PVKTIKTNVVGTLNMLGLAK-------RVGARFLLTSTSEVYGDPLQHPQVETYWGNVN 277

Query: 183 PFHPRSPYAAAKCAAHWYTVNYREAYGIFACNGILFNHESPRR-------GENFVTRKIT 242
           P   RS Y   K  A   T++Y     +      +FN   PR          NFV + + 
Sbjct: 278 PIGVRSCYDEGKRTAETLTMDYHRGANVEVRIARIFNTYGPRMCIDDGRVVSNFVAQALR 337

Query: 243 RAVGRIKIGLQSKLFLGNLQASRDWGFAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEF 302
           +         +     G+ + +R + F  D VE +  +++ E    + +      T+   
Sbjct: 338 K---------EPLTVYGDGKQTRSFQFVSDLVEGLMRLMEGEHVGPFNLGNPGEFTM--- 397

Query: 303 LEVAFGNVGLNWKDHVVID--KRYFRPAEVDN---LKGDASKARKVLGWKPKVGFEKLVK 346
           LE+A   VG       +    K  FRP   D+    K D +KA+++LGW+PKV   + + 
Sbjct: 398 LELAKWMVGEQVVQETIDPNAKIEFRPNTEDDPHKRKPDITKAKELLGWEPKVALRQGLP 428

[Close]

BLAST of CmaCh16G006110 vs. TAIR 10
Match: AT3G62830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 9.8e-09
Identity = 78/337 (23.15%), Postives = 135/337 (40.06%), Query Frame = 0


Query: 26  LITGITGQDGSYLTEFLLEKGYEVHGLIRRSSNFNTQRINHIYIDPHNVHKARMKLHYAD 85
           ++TG  G  GS+L + L+ +G  V  +     NF T R  ++    H+      ++   D
Sbjct: 122 VVTGGAGFVGSHLVDRLMARGDTVIVV----DNFFTGRKENVM---HHFSNPNFEMIRHD 181

Query: 86  LTDASSLRRWLDIIQPDEVYNLAA-QSHVAVSFEIPDYTADVVATGALRLLEAVRSHISA 145
           + +         +++ D++Y+LA   S V   F  P  T      G L +L   +     
Sbjct: 182 VVEPI-------LLEVDQIYHLACPASPVHYKFN-PVKTIKTNVVGTLNMLGLAK----- 241

Query: 146 TGRTQIRYYQAGSSEMFGS-TPPPQSET-----TPFHPRSPYAAAKCAAHWYTVNYREAY 205
             R   R+    +SE++G     PQ ET      P   RS Y   K  A   T++Y    
Sbjct: 242 --RVGARFLLTSTSEVYGDPLQHPQVETYWGNVNPIGVRSCYDEGKRTAETLTMDYHRGA 301

Query: 206 GIFACNGILFNHESPRR-------GENFVTRKITRAVGRIKIGLQSKLFLGNLQASRDWG 265
            +      +FN   PR          NFV + + +         +     G+ + +R + 
Sbjct: 302 NVEVRIARIFNTYGPRMCIDDGRVVSNFVAQALRK---------EPLTVYGDGKQTRSFQ 361

Query: 266 FAGDYVEAMWLMLQQEQPDDYVVATEESHTVEEFLEVAFGNVGLNWKDHVVIDKRYFRPA 325
           F  D VE +  +++ E    + +      T+ E  +V    +  N           FRP 
Sbjct: 362 FVSDLVEGLMRLMEGEHVGPFNLGNPGEFTMLELAKVVQETIDPNANIE-------FRPN 420

Query: 326 EVDN---LKGDASKARKVLGWKPKVGFEKLVKMMVDE 346
             D+    K D +KA+++LGW+PKV   + + +MV +
Sbjct: 422 TEDDPHKRKPDITKAKELLGWEPKVSLRQGLPLMVKD 420

The following BLAST results are available for this feature:

BLAST of CmaCh16G006110 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93031	3.1e-185	85.29	GDP-mannose 4,6 dehydratase 2 OS=Arabidopsis thaliana OX=3702 GN=MUR1 PE=1 SV=3	[more]
Q9SNY3	1.0e-183	86.19	GDP-mannose 4,6 dehydratase 1 OS=Arabidopsis thaliana OX=3702 GN=GMD1 PE=1 SV=1	[more]
P0AC90	3.1e-108	54.50	GDP-mannose 4,6-dehydratase OS=Escherichia coli O157:H7 OX=83334 GN=gmd PE=3 SV=...	[more]
P0AC89	3.1e-108	54.50	GDP-mannose 4,6-dehydratase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 7009...	[more]
P0AC88	3.1e-108	54.50	GDP-mannose 4,6-dehydratase OS=Escherichia coli (strain K12) OX=83333 GN=gmd PE=...	[more]

BLAST of CmaCh16G006110 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51160.1	2.2e-186	85.29	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G66280.1	7.1e-185	86.19	GDP-D-mannose 4,6-dehydratase 1	[more]
AT2G47650.1	1.2e-09	23.69	UDP-xylose synthase 4	[more]
AT2G47650.2	4.4e-09	24.11	UDP-xylose synthase 4	[more]
AT3G62830.1	9.8e-09	23.15	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.25.10		coord: 216..342 e-value: 2.4E-132 score: 442.6
None	No IPR available	GENE3D	3.40.50.720		coord: 26..332 e-value: 2.4E-132 score: 442.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..18
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR43715:SF3	GDP-MANNOSE 4,6 DEHYDRATASE 1-LIKE	coord: 17..363
None	No IPR available	CDD	cd05260	GDP_MD_SDR_e	coord: 24..348 e-value: 0.0 score: 544.885
IPR006368	GDP-mannose 4,6-dehydratase	TIGRFAM	TIGR01472	TIGR01472	coord: 24..349 e-value: 3.2E-171 score: 567.0
IPR006368	GDP-mannose 4,6-dehydratase	PANTHER	PTHR43715	GDP-MANNOSE 4,6-DEHYDRATASE	coord: 17..363
IPR006368	GDP-mannose 4,6-dehydratase	HAMAP	MF_00955	GDP_Man_dehydratase	coord: 23..352 score: 56.419891
IPR016040	NAD(P)-binding domain	PFAM	PF16363	GDP_Man_Dehyd	coord: 26..342 e-value: 1.1E-137 score: 459.0
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 23..348

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G006110.1	CmaCh16G006110.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042351	'de novo' GDP-L-fucose biosynthetic process
biological_process	GO:0019673	GDP-mannose metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008446	GDP-mannose 4,6-dehydratase activity