CmaCh16G004850 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G004850
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	60S ribosomal protein L27
Location	Cma_Chr16: 2464292 .. 2464699 (+)
RNA-Seq Expression	CmaCh16G004850
Synteny	CmaCh16G004850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAAGTTCCTCAAGCAGAACAAAGCCGTGATCATCCTTCAAGGCCGTTACGCCGGCCGGAAGGCGGTCATTATTCGCGCGTTCGATGACGGAACGCGTGATAGGGCTTATGGCCACTGCTTGGTTGCCGGGATAAAAAAGTATCCGGCGAAAGTCATCCGAAAGGACTCCTCCAAGAAGACTGCTAAGAAATCCCGTGTCAAGGCCTTTATCAAGATCGTCAATTACAGGCACTTGATGCCTACCAGGTATACTCTCGATGTCGATCTTAAAGATGTTGTCACTATTGATTCATTGCAATCCAAGGACAAGAAGGTCACTGCTGCTAAGGAGACCAAGAAGAGGTTTGAGGAAAGGTTCAAAACCGGGAAGAACAGGTGGTTTTTCACCAAACTCAGGTTCTAA

mRNA sequence

ATGGTGAAGTTCCTCAAGCAGAACAAAGCCGTGATCATCCTTCAAGGCCGTTACGCCGGCCGGAAGGCGGTCATTATTCGCGCGTTCGATGACGGAACGCGTGATAGGGCTTATGGCCACTGCTTGGTTGCCGGGATAAAAAAGTATCCGGCGAAAGTCATCCGAAAGGACTCCTCCAAGAAGACTGCTAAGAAATCCCGTGTCAAGGCCTTTATCAAGATCGTCAATTACAGGCACTTGATGCCTACCAGGTATACTCTCGATGTCGATCTTAAAGATGTTGTCACTATTGATTCATTGCAATCCAAGGACAAGAAGGTCACTGCTGCTAAGGAGACCAAGAAGAGGTTTGAGGAAAGGTTCAAAACCGGGAAGAACAGGTGGTTTTTCACCAAACTCAGGTTCTAA

Coding sequence (CDS)

ATGGTGAAGTTCCTCAAGCAGAACAAAGCCGTGATCATCCTTCAAGGCCGTTACGCCGGCCGGAAGGCGGTCATTATTCGCGCGTTCGATGACGGAACGCGTGATAGGGCTTATGGCCACTGCTTGGTTGCCGGGATAAAAAAGTATCCGGCGAAAGTCATCCGAAAGGACTCCTCCAAGAAGACTGCTAAGAAATCCCGTGTCAAGGCCTTTATCAAGATCGTCAATTACAGGCACTTGATGCCTACCAGGTATACTCTCGATGTCGATCTTAAAGATGTTGTCACTATTGATTCATTGCAATCCAAGGACAAGAAGGTCACTGCTGCTAAGGAGACCAAGAAGAGGTTTGAGGAAAGGTTCAAAACCGGGAAGAACAGGTGGTTTTTCACCAAACTCAGGTTCTAA

Protein sequence

MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSKKTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEERFKTGKNRWFFTKLRF

Homology

[Close]

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot
Match: Q8LCL3 (60S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 2.2e-59
Identity = 113/135 (83.70%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLKQNKAVI+LQGRYAG+KAVII++FDDGT DR YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK FIK+VNY+HLMPTRYTLDVDLK+V T+D+L+SKDKKVTA KE K + EER
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot
Match: P51419 (60S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2)

HSP 1 Score: 229.6 bits (584), Expect = 2.2e-59
Identity = 113/135 (83.70%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLKQNKAVI+LQGRYAG+KAVII++FDDG RDR YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK FIK+VNY+HLMPTRYTLDVDLK+V T+D+LQSKDKKV A KE K + EER
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot
Match: Q05462 (60S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 1.8e-58
Identity = 112/135 (82.96%), Postives = 125/135 (92.59%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLK NKAVI+LQGRYAG+KAVI++ FDDGTR++ YGHCLVAGIKK+P+KVI+KDS+K
Sbjct: 1   MVKFLKPNKAVILLQGRYAGKKAVIVKTFDDGTREKPYGHCLVAGIKKFPSKVIKKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVKAF+K+VNY+HLMPTRYTLDVDLKD V  D LQSKDKKVTA KETKK  EER
Sbjct: 61  KTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKDAVVPDVLQSKDKKVTALKETKKSLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot
Match: Q9SKX8 (60S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.1e-54
Identity = 101/135 (74.81%), Postives = 122/135 (90.37%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVK +K  KAVI+LQGRY G+KAVI+++FDDGT ++ YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK F K++NY+H+MPTRYTLD+DLK+VV+ D++ SKDKKVTA KE K +FEER
Sbjct: 61  KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot
Match: P41101 (60S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 7.4e-52
Identity = 107/139 (76.98%), Postives = 118/139 (84.89%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           M KFLK NKAVI+LQG+YAGRKAVI+RAFD+GTRDR YGHCLVAGI +YP KVIRKDS+K
Sbjct: 1   MEKFLKPNKAVILLQGKYAGRKAVIVRAFDEGTRDRPYGHCLVAGISRYPKKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDL----KDVVTIDSLQSKDKKVTAAKETKKR 120
           K  KKSRVKAFIK+VNY H+MPTRYTLD DL    KDVV  D LQ++DKKVT A+ TK R
Sbjct: 61  KAGKKSRVKAFIKLVNYNHIMPTRYTLDEDLKDVVKDVVNADVLQARDKKVT-ARRTKAR 120

Query: 121 FEERFKTGKNRWFFTKLRF 136
             ERFKTGKNRWFFTKLRF
Sbjct: 121 LAERFKTGKNRWFFTKLRF 138

[Close]

BLAST of CmaCh16G004850 vs. TAIR 10
Match: AT3G22230.1 (Ribosomal L27e protein family )

HSP 1 Score: 229.6 bits (584), Expect = 1.5e-60
Identity = 113/135 (83.70%), Postives = 127/135 (94.07%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLKQNKAVI+LQGRYAG+KAVII++FDDGT DR YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGTSDRRYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK FIK+VNY+HLMPTRYTLDVDLK+V T+D+L+SKDKKVTA KE K + EER
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. TAIR 10
Match: AT4G15000.1 (Ribosomal L27e protein family )

HSP 1 Score: 229.6 bits (584), Expect = 1.5e-60
Identity = 113/135 (83.70%), Postives = 126/135 (93.33%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLKQNKAVI+LQGRYAG+KAVII++FDDG RDR YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK FIK+VNY+HLMPTRYTLDVDLK+V T+D+LQSKDKKV A KE K + EER
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. TAIR 10
Match: AT2G32220.1 (Ribosomal L27e protein family )

HSP 1 Score: 211.5 bits (537), Expect = 4.3e-55
Identity = 101/135 (74.81%), Postives = 122/135 (90.37%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVK +K  KAVI+LQGRY G+KAVI+++FDDGT ++ YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKCMKPGKAVILLQGRYTGKKAVIVKSFDDGTVEKKYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK F K++NY+H+MPTRYTLD+DLK+VV+ D++ SKDKKVTA KE K +FEER
Sbjct: 61  KTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKEAKAKFEER 120

Query: 121 FKTGKNRWFFTKLRF 136
           FKTGKNRWFFTKLRF
Sbjct: 121 FKTGKNRWFFTKLRF 135

[Close]

BLAST of CmaCh16G004850 vs. TAIR 10
Match: AT4G15000.2 (Ribosomal L27e protein family )

HSP 1 Score: 204.1 bits (518), Expect = 6.9e-53
Identity = 101/123 (82.11%), Postives = 114/123 (92.68%), Query Frame = 0


Query: 1   MVKFLKQNKAVIILQGRYAGRKAVIIRAFDDGTRDRAYGHCLVAGIKKYPAKVIRKDSSK 60
           MVKFLKQNKAVI+LQGRYAG+KAVII++FDDG RDR YGHCLVAG+KKYP+KVIRKDS+K
Sbjct: 1   MVKFLKQNKAVILLQGRYAGKKAVIIKSFDDGNRDRPYGHCLVAGLKKYPSKVIRKDSAK 60

Query: 61  KTAKKSRVKAFIKIVNYRHLMPTRYTLDVDLKDVVTIDSLQSKDKKVTAAKETKKRFEER 120
           KTAKKSRVK FIK+VNY+HLMPTRYTLDVDLK+V T+D+LQSKDKKV A KE K + EER
Sbjct: 61  KTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKEAKAKLEER 120

Query: 121 FKT 124
           FKT
Sbjct: 121 FKT 123

The following BLAST results are available for this feature:

BLAST of CmaCh16G004850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LCL3	2.2e-59	83.70	60S ribosomal protein L27-2 OS=Arabidopsis thaliana OX=3702 GN=RPL27B PE=2 SV=2	[more]
P51419	2.2e-59	83.70	60S ribosomal protein L27-3 OS=Arabidopsis thaliana OX=3702 GN=RPL27C PE=2 SV=2	[more]
Q05462	1.8e-58	82.96	60S ribosomal protein L27 OS=Pisum sativum OX=3888 GN=RPL27 PE=2 SV=1	[more]
Q9SKX8	6.1e-54	74.81	60S ribosomal protein L27-1 OS=Arabidopsis thaliana OX=3702 GN=RPL27A PE=2 SV=1	[more]
P41101	7.4e-52	76.98	60S ribosomal protein L27 OS=Solanum tuberosum OX=4113 GN=RPL27 PE=2 SV=1	[more]

BLAST of CmaCh16G004850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22230.1	1.5e-60	83.70	Ribosomal L27e protein family	[more]
AT4G15000.1	1.5e-60	83.70	Ribosomal L27e protein family	[more]
AT2G32220.1	4.3e-55	74.81	Ribosomal L27e protein family	[more]
AT4G15000.2	6.9e-53	82.11	Ribosomal L27e protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 100..120
None	No IPR available	PANTHER	PTHR10497:SF16	60S RIBOSOMAL PROTEIN L27	coord: 1..135
IPR038655	Ribosomal protein L27e superfamily	GENE3D	2.30.30.770		coord: 1..135 e-value: 2.2E-59 score: 201.4
IPR001141	Ribosomal protein L27e	PFAM	PF01777	Ribosomal_L27e	coord: 52..135 e-value: 2.3E-31 score: 108.0
IPR001141	Ribosomal protein L27e	PANTHER	PTHR10497	60S RIBOSOMAL PROTEIN L27	coord: 1..135
IPR018262	Ribosomal protein L27e, conserved site	PROSITE	PS01107	RIBOSOMAL_L27E	coord: 124..135
IPR041991	Eukaryotic Ribosomal Protein L27, KOW domain	CDD	cd06090	KOW_RPL27	coord: 6..88 e-value: 6.78157E-40 score: 126.914
IPR008991	Translation protein SH3-like domain superfamily	SUPERFAMILY	50104	Translation proteins SH3-like domain	coord: 3..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G004850.1	CmaCh16G004850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006412	translation
cellular_component	GO:0022625	cytosolic large ribosomal subunit
cellular_component	GO:0005840	ribosome
molecular_function	GO:0003735	structural constituent of ribosome