CmaCh16G004810 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G004810 vs. ExPASy Swiss-Prot
Match: Q94C69 (Cold shock domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=CSP3 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-08
Identity = 73/257 (28.40%), Postives = 92/257 (35.80%), Query Frame = 0

Query: 98  SANTTAHAQSDGGGSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEKSC-PCG---- 157
           S N   ++    GG+CF CG++GH A+DCD    G SF       S  E  C  CG    
Sbjct: 81  SLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGGGRRSGGEGECYMCGDVGH 140

Query: 158 --RGVCVVLTANT--ERNRGRKFYKCPVRQENGGCGFF-EWCDNASATNIMTYGSQNRAS 217
             R        N+      GR  Y C      G  G   + C   S  N   YG      
Sbjct: 141 FARDCRQSGGGNSGGGGGGGRPCYSC------GEVGHLAKDCRGGSGGN--RYGGGGGRG 200

Query: 218 SSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEPSMAAKNQVSA 277
           S       C              G NVG     C     TCG                  
Sbjct: 201 SGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTC----YTCG-------------GVGHI 260

Query: 278 SKVYGNTTDTSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNPPPAEFGRNQSSSAG 337
           +KV      TSK        G +CY+CGG GH ARDC +  + S+             + 
Sbjct: 261 AKVC-----TSKIPSGGGGGGRACYECGGTGHLARDCDRRGSGSS--------GGGGGSN 299

Query: 338 SCFKCGKPGHWARDCSN 345
            CF CGK GH+AR+C++
Sbjct: 321 KCFICGKEGHFARECTS 299

BLAST of CmaCh16G004810 vs. ExPASy Swiss-Prot
Match: O65639 (Cold shock protein 1 OS=Arabidopsis thaliana OX=3702 GN=CSP1 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-07
Identity = 60/247 (24.29%), Postives = 86/247 (34.82%), Query Frame = 0

Query: 106 QSDGGGSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEKSCPCGRGVCVVLTANTER 165
           +S GG  C+ CG  GH+ARDC + G+G    A    T      C     V  V    T++
Sbjct: 127 RSRGGEGCYNCGDTGHFARDCTSAGNGDQRGA----TKGGNDGCYTCGDVGHVARDCTQK 186

Query: 166 NRGRKFYKCPVRQENGGC------GFF--EWCDNASATNIMTYGSQNRASSSSFSDLKCP 225
           + G    +  V+  N GC      G F  +     +A N+ + G                
Sbjct: 187 SVGNGDQRGAVKGGNDGCYTCGDVGHFARDCTQKVAAGNVRSGGG--------------- 246

Query: 226 CGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEPSMAAKNQVSASKVYGNTTDT 285
            G+G+C               Y C                              G     
Sbjct: 247 -GSGTC---------------YSC------------------------------GGVGHI 297

Query: 286 SKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGH 345
           ++      +    CY+CGG GH ARDC Q            G     +  +C+KCGK GH
Sbjct: 307 ARDCATKRQPSRGCYQCGGSGHLARDCDQR-----------GSGGGGNDNACYKCGKEGH 297

BLAST of CmaCh16G004810 vs. ExPASy Swiss-Prot
Match: Q9NG98 (DNA topoisomerase 3-alpha OS=Drosophila melanogaster OX=7227 GN=Top3alpha PE=2 SV=2)

HSP 1 Score: 55.5 bits (132), Expect = 1.4e-06
Identity = 53/202 (26.24%), Postives = 73/202 (36.14%), Query Frame = 0

Query: 166  NRGRKFYKCPVRQENGGCGFFEWCD----------------------------------- 225
            N GR +YKCP   E   C FF+W D                                   
Sbjct: 1053 NLGRLYYKCPKPDE---CNFFQWADEPPSSAKSKNSTGSAPQSTTSWGSNRVVTLPSIQQ 1112

Query: 226  ------------NASATNIMT-----YGSQNRASSSSFSDLKCPCGAGSCITLTAKTGEN 285
                        N+S+T  +T        +N A+     ++ C CG  +      K G N
Sbjct: 1113 SNSQRGQSSMRSNSSSTVTITQTKTKQQERNTATPGDGEEVMCNCGQLASQLTVRKDGPN 1172

Query: 286  VGQQFYRCPSYQATCGFFQWCKEPSMAAKNQVSASKVYGN-----------TTDTSKGMP 305
             G+ FY CP+ + +CGFF+W  E     +NQ ++S  +G+           T  TS G P
Sbjct: 1173 QGRPFYACPTREKSCGFFKWGDED----QNQGASSTSWGSANRNPPGRSQPTAITSDG-P 1232

BLAST of CmaCh16G004810 vs. ExPASy Swiss-Prot
Match: P27484 (Glycine-rich protein 2 OS=Nicotiana sylvestris OX=4096 GN=GRP-2 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 3.2e-06
Identity = 26/57 (45.61%), Postives = 34/57 (59.65%), Query Frame = 0

Query: 288 GSSCYKCGGEGHWARDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGHWARDCSN 345
           GS C+KCG  GH+ARDCSQS           G       G C+KCG+ GH+AR+C++
Sbjct: 156 GSGCFKCGESGHFARDCSQS-GGGGGGGRFGGGGGGGGGGGCYKCGEDGHFARECTS 211

BLAST of CmaCh16G004810 vs. ExPASy Swiss-Prot
Match: Q41188 (Cold shock protein 2 OS=Arabidopsis thaliana OX=3702 GN=CSP2 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 7.1e-06
Identity = 31/90 (34.44%), Postives = 38/90 (42.22%), Query Frame = 0

Query: 271 YGNTTDTSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNPPPAEF------------ 330
           YG       G     R GS CYKCG  GH ARDCS+           +            
Sbjct: 111 YGGGGGGYGGRGGGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYGGGGGYGGGGGG 170

Query: 331 ----GRNQSSSAGSCFKCGKPGHWARDCSN 345
               GR      GSC+ CG+ GH+ARDC++
Sbjct: 171 YGGGGRGGGGGGGSCYSCGESGHFARDCTS 200

BLAST of CmaCh16G004810 vs. TAIR 10
Match: AT3G42860.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.8e-63
Identity = 139/331 (41.99%), Postives = 183/331 (55.29%), Query Frame = 0

Query: 17  DDDEDFLSEVAAAEARALASKRR--KIPNHTINPPKRAIAEVTNKVSPNVDAEGIYIAAL 76
           D+++DFL ++AA EA A A + R   IP                        EG Y+AAL
Sbjct: 12  DEEDDFLLQLAAIEAEAAAKRPRVSSIP------------------------EGPYMAAL 71

Query: 77  KGDPIILSQLSGRIANSGNKGFISANTTAHAQSDGGGSCFKCGKLGHWARDCDAPGSGGS 136
           KG      Q S     S ++  ++  T    +SDGGG                       
Sbjct: 72  KGSKSDQWQQSPLNPASKSRS-VAVTTGGFQRSDGGGGV--------------------- 131

Query: 137 FSASENDTSVAEKSCPCGRGVCVVLTANTERNRGRKFYKCPVRQENGGCGFFEWCD--NA 196
             A E D    EKSCPCG G+C++LT+NT +N GRKFYKCP R+ENGGCGFF+WCD   +
Sbjct: 132 --AGEQD--FPEKSCPCGVGICLILTSNTPKNPGRKFYKCPNREENGGCGFFQWCDAVQS 191

Query: 197 SATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQ 256
           S T+  T  S    + + F D +CPCGAG C  LTAKTGENVG+QFYRCP ++ +CGFF+
Sbjct: 192 SGTSTTTSNSYGNGNDTKFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCPVFEGSCGFFK 251

Query: 257 WCKEPSMAAKNQVSASKVYGNTTDTSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSN 316
           WC +  +++    S +K   N  D+       A+ G+ CYKCG EGHWARDC+     S+
Sbjct: 252 WCNDNVVSSPTSYSVTK-NSNFGDSDTRGYQNAKTGTPCYKCGKEGHWARDCT---VQSD 284

Query: 317 PPPAEFGRNQSSSAGSCFKCGKPGHWARDCS 344
             P +     +S+AG CFKCGKPGHW+RDC+
Sbjct: 312 TGPVK----STSAAGDCFKCGKPGHWSRDCT 284

BLAST of CmaCh16G004810 vs. TAIR 10
Match: AT5G13920.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 51/145 (35.17%), Postives = 65/145 (44.83%), Query Frame = 0

Query: 111 GSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEKSCPCGRGVCVVLTANTERNRGRK 170
           G CF+C + GHW  DC           S  D       CPCG G C +  ANT  N GRK
Sbjct: 9   GDCFRCRQAGHWINDCPL--------KSYTDDPPPAIQCPCGGGFCEIKVANTRENPGRK 68

Query: 171 FYKCPVRQENGGCGFFEWCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKT 230
           FYKCP  Q    C FF+WCD  +  +I            +F+   C CGAG C     + 
Sbjct: 69  FYKCPTAQ---NCCFFKWCDKVTDEDI--------KFRPAFTIPICSCGAGPC----RRV 128

Query: 231 GENVGQQFYRCPSYQ--ATCGFFQW 254
            +  G+ +  C   +    CGFF+W
Sbjct: 129 KDVSGRAYLICCIKKGFGACGFFKW 130

BLAST of CmaCh16G004810 vs. TAIR 10
Match: AT2G17870.1 (cold shock domain protein 3 )

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-09
Identity = 73/257 (28.40%), Postives = 92/257 (35.80%), Query Frame = 0

Query: 98  SANTTAHAQSDGGGSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEKSC-PCG---- 157
           S N   ++    GG+CF CG++GH A+DCD    G SF       S  E  C  CG    
Sbjct: 81  SLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGGGRRSGGEGECYMCGDVGH 140

Query: 158 --RGVCVVLTANT--ERNRGRKFYKCPVRQENGGCGFF-EWCDNASATNIMTYGSQNRAS 217
             R        N+      GR  Y C      G  G   + C   S  N   YG      
Sbjct: 141 FARDCRQSGGGNSGGGGGGGRPCYSC------GEVGHLAKDCRGGSGGN--RYGGGGGRG 200

Query: 218 SSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEPSMAAKNQVSA 277
           S       C              G NVG     C     TCG                  
Sbjct: 201 SGGDGCYMCGGVGHFARDCRQNGGGNVGGGGSTC----YTCG-------------GVGHI 260

Query: 278 SKVYGNTTDTSKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNPPPAEFGRNQSSSAG 337
           +KV      TSK        G +CY+CGG GH ARDC +  + S+             + 
Sbjct: 261 AKVC-----TSKIPSGGGGGGRACYECGGTGHLARDCDRRGSGSS--------GGGGGSN 299

Query: 338 SCFKCGKPGHWARDCSN 345
            CF CGK GH+AR+C++
Sbjct: 321 KCFICGKEGHFARECTS 299

BLAST of CmaCh16G004810 vs. TAIR 10
Match: AT4G36020.1 (cold shock domain protein 1 )

HSP 1 Score: 58.9 bits (141), Expect = 9.2e-09
Identity = 60/247 (24.29%), Postives = 86/247 (34.82%), Query Frame = 0

Query: 106 QSDGGGSCFKCGKLGHWARDCDAPGSGGSFSASENDTSVAEKSCPCGRGVCVVLTANTER 165
           +S GG  C+ CG  GH+ARDC + G+G    A    T      C     V  V    T++
Sbjct: 127 RSRGGEGCYNCGDTGHFARDCTSAGNGDQRGA----TKGGNDGCYTCGDVGHVARDCTQK 186

Query: 166 NRGRKFYKCPVRQENGGC------GFF--EWCDNASATNIMTYGSQNRASSSSFSDLKCP 225
           + G    +  V+  N GC      G F  +     +A N+ + G                
Sbjct: 187 SVGNGDQRGAVKGGNDGCYTCGDVGHFARDCTQKVAAGNVRSGGG--------------- 246

Query: 226 CGAGSCITLTAKTGENVGQQFYRCPSYQATCGFFQWCKEPSMAAKNQVSASKVYGNTTDT 285
            G+G+C               Y C                              G     
Sbjct: 247 -GSGTC---------------YSC------------------------------GGVGHI 297

Query: 286 SKGMPATARAGSSCYKCGGEGHWARDCSQSLAPSNPPPAEFGRNQSSSAGSCFKCGKPGH 345
           ++      +    CY+CGG GH ARDC Q            G     +  +C+KCGK GH
Sbjct: 307 ARDCATKRQPSRGCYQCGGSGHLARDCDQR-----------GSGGGGNDNACYKCGKEGH 297

BLAST of CmaCh16G004810 vs. TAIR 10
Match: AT1G54930.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-08
Identity = 27/69 (39.13%), Postives = 39/69 (56.52%), Query Frame = 0

Query: 188 WCDNASATNIMTYGSQNRASSSSFSDLKCPCGAGSCITLTAKTGENVGQQFYRCPSYQAT 247
           W  N     + T      A++ S  D+ CPC AG C T+T+KT +N  ++FY CPS    
Sbjct: 15  WAKNCP---LKTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCPS---- 74

Query: 248 CGFFQWCKE 257
           CG+F+WC +
Sbjct: 75  CGYFKWCDQ 76