CmaCh16G004800 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh16G004800 vs. ExPASy Swiss-Prot
Match: Q54PM7 (N-acetyl-D-glucosamine kinase OS=Dictyostelium discoideum OX=44689 GN=nagk PE=3 SV=2)

HSP 1 Score: 189.5 bits (480), Expect = 6.6e-47
Identity = 119/331 (35.95%), Postives = 181/331 (54.68%), Query Frame = 0

Query: 36  VILGIDGGTTSTVCVCVPLLPLQSLHLPDPLPLLARVEAGCSNHNSVGETAARETLEQ-- 95
           + +GIDGG T T  V V     +          LAR  + CSN++SVGE  A+  + +  
Sbjct: 5   IFIGIDGGGTKTSTVAVDSNGQE----------LARHTSPCSNYHSVGEDLAKAAINEGI 64

Query: 96  -----VMAEALSKSGSDRSAVQAICLSVSGVNHPTDQQRILNWLRDMFPSHVKLYVRNDA 155
                 + E ++   +    V +ICL +SGV+   D+  + +W+ ++    +   + NDA
Sbjct: 65  KYVIRKVKETITDDDNKEVTVGSICLGMSGVDREKDKLLVKSWVTELLGESINYSIHNDA 124

Query: 156 AAALASGTMGRLSGCVLIAGTGSIAFGFTDDGREARAAGAGPILGDWGSGYGISAQALTA 215
             AL+SGT G+L G V+I GTG I+ GF  +G   R+ G GP+LGD+GSGY I    L  
Sbjct: 125 IVALSSGTQGKLFGVVIICGTGCISLGFNREGVSGRSGGWGPLLGDYGSGYQIGYDILRH 184

Query: 216 IIRAHDGRGPQTNLTNSILQTLGLSSADELIGWTY--ADSSWARIAALVPAVVSCAEAGD 275
           +++A D  GP+T+LT  +L+ L L+  ++LI W Y     SW + A L P     A+ GD
Sbjct: 185 VLKAKDQVGPKTSLTQVLLEKLQLTKEEDLISWAYDPKTQSWQKFAQLSPLAFEQAQLGD 244

Query: 276 EVANNILQDAVKELALSVNAVVQRLGFSGSDGKGSFPLVMVGGVIEGNKGWGIAQEVIN- 335
           E++N IL DA   L   +N+V+++LG    D +  FPLV  GG IE     GI  ++++ 
Sbjct: 245 EISNLILVDAANALYDLINSVIKKLGL---DKEEKFPLVYTGGNIERK---GILSDLLSK 304

Query: 336 CISKDYPGVVPIWPKVEPAIGAALLAWNFLK 357
            I ++YP    +    +P++GAALLA N  K
Sbjct: 305 KIMENYPNAEILNTTCDPSMGAALLALNSKK 319

BLAST of CmaCh16G004800 vs. ExPASy Swiss-Prot
Match: Q97ML3 (N-acetylmuramic acid/N-acetylglucosamine kinase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) OX=272562 GN=murK PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 8.7e-31
Identity = 109/316 (34.49%), Postives = 160/316 (50.63%), Query Frame = 0

Query: 37  ILGIDGGTTSTVCVCVPLLPLQSLHLPDPLPLLARVEAGCSNHNSVGETAARETLEQVMA 96
           ++GIDGG + T       + + +L       +L  V  G SN NS  +   +  L++++ 
Sbjct: 4   VIGIDGGGSKT------HMKISTL----DYKVLLEVFKGPSNINSSTKEEVKRVLQELIM 63

Query: 97  EALSKSGSDRSAVQAICLSVSGVNHPTDQQRILNWLRDMFPSHVKLYVRNDAAAALASGT 156
           E L K G       AIC+  +G +   D+  I + +R +     K+ V NDA  ALA G 
Sbjct: 64  EGLGKLGQSLEECSAICIGTAGADRTEDKSIIEDMIRSL-GYMGKIIVVNDAEIALAGGI 123

Query: 157 MGRLSGCVLIAGTGSIAFGFTDDGREARAAGAGPILGDWGSGYGISAQALTAIIRAHDGR 216
             R  G ++I+GTGSI +G   +GR AR+ G G I+GD GSGY I  +A+ A +++ D R
Sbjct: 124 EKR-EGIIVISGTGSICYGRNKEGRSARSGGWGHIIGDEGSGYDIGIKAIKAALKSFDKR 183

Query: 217 GPQTNLTNSILQTLGLSSADELIGWTYADS-SWARIAALVPAVVSCAEAGDEVANNILQD 276
           G +T L   IL  L L S ++LI + Y    +   IA+L   V S    GD V+  IL++
Sbjct: 184 GEKTILEGDILDFLKLKSHEDLINYIYRSGVTKKEIASLTRVVNSAYIKGDLVSKRILKE 243

Query: 277 AVKELALSVNAVVQRLGFSGSDGKGSFPLVMVGGVIEGNKGWGIAQEVINCISKDYPGVV 336
           A +EL LSV AVV+ L            L   GGVI  N    +  E    ++ +YP V 
Sbjct: 244 AARELFLSVKAVVEVLSMQNK----KVVLTTAGGVI--NNINYLYDEFRKFLNLNYPKVK 301

Query: 337 PIWPKVEPAIGAALLA 352
            I  K + A GA ++A
Sbjct: 304 IISMKNDSAFGAVIIA 301

BLAST of CmaCh16G004800 vs. ExPASy Swiss-Prot
Match: Q3SZM9 (N-acetyl-D-glucosamine kinase OS=Bos taurus OX=9913 GN=NAGK PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.2e-11
Identity = 76/290 (26.21%), Postives = 130/290 (44.83%), Query Frame = 0

Query: 39  GIDGGTTSTVCVCVPLLPLQSLHLPDPLPLLARVEAGCSNHNSVGETAARETLEQVMAEA 98
           G++GG T +          + L L +   +LA  +   +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRS----------KVLLLSEDGQILAEADGLSTNHWLIGTDKCVERINEMVNRA 66

Query: 99  LSKSGSDRSA-VQAICLSVSGVNHPTDQQRILNWLRDMFPSHVKLY-VRNDAAAALASGT 158
             K+G D    ++ + LS+SG +     + ++  LRD FP   + Y +  DAA ++A+ T
Sbjct: 67  KRKAGVDPLVPLRGLGLSLSGGDQEDAVRMLMEELRDRFPYLSESYLITTDAAGSIATAT 126

Query: 159 MGRLSGCVLIAGTGSIAFGFTDDGREARAAGAGPILGDWGSGYGISAQALTAIIRAHDGR 218
                G VLI+GTGS       DG E+   G G ++GD GS Y I+ QA+  +  + D  
Sbjct: 127 PD--GGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 219 GPQTNLTNSILQTL----GLSSADELIGWTYADSSWARIAALVPAVVSCAEAGDEVANNI 278
               +    + Q +     +     ++   Y D   +R A     V   A+ GD ++  I
Sbjct: 187 EAAPHDIGYVKQAMFNYFQVPDRLGILTHLYRDFDKSRFAGFCRKVAEGAQQGDPLSRCI 246

Query: 279 LQDAVKELALSVNAVVQRLGFSGSDGKGSFPLVMVGGVIEGNKGWGIAQE 323
            + A + L   V AV+  +      G+   P++ VG V    K W + +E
Sbjct: 247 FRKAGEMLGRHVVAVLPEIDPVLFQGEMGLPILCVGSVW---KSWELLKE 281

BLAST of CmaCh16G004800 vs. ExPASy Swiss-Prot
Match: Q9UJ70 (N-acetyl-D-glucosamine kinase OS=Homo sapiens OX=9606 GN=NAGK PE=1 SV=4)

HSP 1 Score: 71.6 bits (174), Expect = 2.0e-11
Identity = 74/290 (25.52%), Postives = 130/290 (44.83%), Query Frame = 0

Query: 39  GIDGGTTSTVCVCVPLLPLQSLHLPDPLPLLARVEAGCSNHNSVGETAARETLEQVMAEA 98
           G++GG T +          + L + +   +LA  +   +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRS----------EVLLVSEDGKILAEADGLSTNHWLIGTDKCVERINEMVNRA 66

Query: 99  LSKSGSDRSA-VQAICLSVSGVNHPTDQQRILNWLRDMFPSHVKLY-VRNDAAAALASGT 158
             K+G D    ++++ LS+SG +     + ++  LRD FP   + Y +  DAA ++A+ T
Sbjct: 67  KRKAGVDPLVPLRSLGLSLSGGDQEDAGRILIEELRDRFPYLSESYLITTDAAGSIATAT 126

Query: 159 MGRLSGCVLIAGTGSIAFGFTDDGREARAAGAGPILGDWGSGYGISAQALTAIIRAHDGR 218
                G VLI+GTGS       DG E+   G G ++GD GS Y I+ QA+  +  + D  
Sbjct: 127 PD--GGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 219 GPQTNLTNSILQTL----GLSSADELIGWTYADSSWARIAALVPAVVSCAEAGDEVANNI 278
               +    + Q +     +     ++   Y D    R A     +   A+ GD ++  I
Sbjct: 187 EAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDKCRFAGFCRKIAEGAQQGDPLSRYI 246

Query: 279 LQDAVKELALSVNAVVQRLGFSGSDGKGSFPLVMVGGVIEGNKGWGIAQE 323
            + A + L   + AV+  +      GK   P++ VG V    K W + +E
Sbjct: 247 FRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVW---KSWELLKE 281

BLAST of CmaCh16G004800 vs. ExPASy Swiss-Prot
Match: P81799 (N-acetyl-D-glucosamine kinase OS=Rattus norvegicus OX=10116 GN=Nagk PE=1 SV=4)

HSP 1 Score: 70.9 bits (172), Expect = 3.4e-11
Identity = 74/290 (25.52%), Postives = 130/290 (44.83%), Query Frame = 0

Query: 39  GIDGGTTSTVCVCVPLLPLQSLHLPDPLPLLARVEAGCSNHNSVGETAARETLEQVMAEA 98
           G++GG T +          + L L +   +LA  +   +NH  +G     E + +++  A
Sbjct: 7   GVEGGGTRS----------KVLLLSEDGQILAEADGLSTNHWLIGTGTCVERINEMVDRA 66

Query: 99  LSKSGSDRSA-VQAICLSVSGVNHPTDQQRILNWLRDMFPSHVKLY-VRNDAAAALASGT 158
             K+G D    ++++ LS+SG       + ++  LRD FP   + Y +  DAA ++A+ T
Sbjct: 67  KRKAGVDPLVPLRSLGLSLSGGEQEDAVRLLMEELRDRFPYLSESYFITTDAAGSIATAT 126

Query: 159 MGRLSGCVLIAGTGSIAFGFTDDGREARAAGAGPILGDWGSGYGISAQALTAIIRAHDGR 218
                G VLI+GTGS       DG E+   G G ++GD GS Y I+ QA+  +  + D  
Sbjct: 127 PD--GGIVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIAHQAVKIVFDSIDNL 186

Query: 219 GPQTNLTNSILQTL----GLSSADELIGWTYADSSWARIAALVPAVVSCAEAGDEVANNI 278
               +    + Q +     +     ++   Y D   ++ A     +   A+ GD ++  I
Sbjct: 187 EAAPHDIGHVKQAMFNYFQVPDRLGILTHLYRDFDKSKFAGFCQKIAEGAQQGDPLSRFI 246

Query: 279 LQDAVKELALSVNAVVQRLGFSGSDGKGSFPLVMVGGVIEGNKGWGIAQE 323
            + A + L   V AV+  +      G+   P++ VG V    K W + +E
Sbjct: 247 FRKAGEMLGRHVVAVLPEIDPVLFQGELGLPILCVGSVW---KSWELLKE 281

BLAST of CmaCh16G004800 vs. TAIR 10
Match: AT1G30540.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 453.8 bits (1166), Expect = 1.3e-127
Identity = 236/355 (66.48%), Postives = 280/355 (78.87%), Query Frame = 0

Query: 1   MTKKYRNGEIWEFEREMSGRTGGGGGGGGGGGVGGVILGIDGGTTSTVCVCVPLLPLQSL 60
           M   + NG + + E +     GGG      G V GVILG+DGG TSTVCVCVP       
Sbjct: 1   MRNPHSNGNLRKLEAD-----GGGEATEENGFVNGVILGLDGGATSTVCVCVPFFSFGE- 60

Query: 61  HLPDPLPLLARVEAGCSNHNSVGETAARETLEQVMAEALSKSGSDRSAVQAICLSVSGVN 120
             PDPLP+L R  AGC+N NSVGETAAR++LEQV++EAL +SG D+S V+ +CL VSGVN
Sbjct: 61  RFPDPLPILGRAVAGCTNRNSVGETAARDSLEQVISEALVQSGFDKSDVRGVCLGVSGVN 120

Query: 121 HPTDQQRILNWLRDMFPSHVKLYVRNDAAAALASGTMGRLSGCVLIAGTGSIAFGFTDDG 180
           HP+DQ++I NW+RDMFPSHVK+YV+NDA  ALASGTMG+L GCVLIAGTG IA+GF +DG
Sbjct: 121 HPSDQEKIENWIRDMFPSHVKVYVQNDAIVALASGTMGKLHGCVLIAGTGCIAYGFDEDG 180

Query: 181 REARAAGAGPILGDWGSGYGISAQALTAIIRAHDGRGPQTNLTNSILQTLGLSSADELIG 240
           +EARA+G GPILGDWGSGYGI+AQALTA+IRAHDGRGPQT LT++IL+ LGLSS DELIG
Sbjct: 181 KEARASGGGPILGDWGSGYGIAAQALTAVIRAHDGRGPQTMLTSTILKALGLSSPDELIG 240

Query: 241 WTYADSSWARIAALVPAVVSCAEAGDEVANNILQDAVKELALSVNAVVQRLGFSGSDGKG 300
           WTYAD SWARIAALVP VVSCAEAGDE+++ IL DA ++LALSV AVVQRLG  G DG  
Sbjct: 241 WTYADPSWARIAALVPQVVSCAEAGDEISDKILVDAAEDLALSVKAVVQRLGLCGKDGTA 300

Query: 301 SFPLVMVGGVIEGNKGWGIAQEVINCISKDYPGVVPIWPKVEPAIGAALLAWNFL 356
           SFP+VMVGGV+  N+ W I +EV   I++ +PG   I PKVEPA+GAALLA NFL
Sbjct: 301 SFPVVMVGGVLNANQKWDIGKEVSKRINRYFPGAQTIIPKVEPAVGAALLAMNFL 349