CmaCh16G004600 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G004600
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description9-cis-epoxycarotenoid dioxygenase, chloroplastic
LocationCma_Chr16: 2304169 .. 2305953 (+)
RNA-Seq ExpressionCmaCh16G004600
SyntenyCmaCh16G004600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTTGCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTTAATGTCGGAGCGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCACAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTGCCGGTTCCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGTGGAGAGGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGCCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGAGTATTCGGGGGATTTATGTGCGGAACGGTGCGAACCCACTTCATGAACCGGTGTCCGGCCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGAAGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCTAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTCACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATCTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCGACAGACGCCACCGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATTGTGGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGCAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCTGGAATGGTGAACCGAAATCGACTCGGGAGAAAAACCCAGTTCGCTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCTGGTTTTGTGAAGGTCGATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTCCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTAACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGTAA

mRNA sequence

ATGGCTTCTCTTGCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTTAATGTCGGAGCGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCACAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTGCCGGTTCCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGTGGAGAGGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGCCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGAGTATTCGGGGGATTTATGTGCGGAACGGTGCGAACCCACTTCATGAACCGGTGTCCGGCCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGAAGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCTAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTCACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATCTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCGACAGACGCCACCGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATTGTGGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGCAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCTGGAATGGTGAACCGAAATCGACTCGGGAGAAAAACCCAGTTCGCTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCTGGTTTTGTGAAGGTCGATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTCCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTAACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGTAA

Coding sequence (CDS)

ATGGCTTCTCTTGCTTCTTCTTCTTCTTCTTCTTCGTGGGTCAAATCTGGTTTTTCCTCTTTAATGTCGGAGCGTTTCTCTGTAAATATGGATTCCGGCAGGAACAGAGTCGCCTGTTCTCTTCATACTCCTTCCATAATTCAAATTCCTAAGCGTTCACAGACTGCATTTCGGTCTCCGGTTAAGACCACGGTGCCGAAACCGGTGCCGGTTCCGGCTCCGTCAGTGTCGGAGGAGTGGAATTTCTTGCAGCGGGCTGCGGCGATGGCGCTTGATGCTGTTGAGGATGCTCTGGTTTCGGTGGAGAGGAAGCATTCGTTGCCGAAGACGGCGGACCCTTCTGTTCAAATTGCTGGGAATTTTGCTCCGGTGCCGGAGCAGCCGGTTCGGAAGGGGCTGCCGGTGATTGGGAAAGTTCCGGAGAGTATTCGGGGGATTTATGTGCGGAACGGTGCGAACCCACTTCATGAACCGGTGTCCGGCCACCACTTGTTTGATGGTGATGGGATGGTTCATGCTGTGGAGTTCACTGAGGACGGCGAAGTTAGTTATGCTTGCCGGTTTACGGAGACTCAACGGCTGGTTCAGGAACGAGCTTATGGCCGGCCGGTTTTTCCGAAAGCCATTGGAGAACTTCATGGCCACTCTGGTATAGCTCGGCTGTTGCTTTTCTATGCTAGAGGTTTATTTGGGCTGGTCGATCACAGCCATGGAATTGGAGTCGCCAACGCCGGTTTGGTTTATTTCAACGGCCAGCTTCTCGCCATGTCGGAGGATGATTTGCCTTACGAAATCAGAGTCACTCCGGCAGGGGATTTGAAAACCGTTGGCCGGTTTGATTTCGACGGCCAATTGAAATCCACAATGATAGCCCACCCAAAACTCGACCCTGTTTCCGGCGAGATGTTTGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGGGAAAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCAACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCATAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGTTCTCCGGTGATCTATGACAAAGAAAAAACCTCCCGATTTGGAGTTATGGACAAAAATGCGACAGACGCCACCGCCATTAAATGGATCGACGTTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACAGAGGAAATTGTGGTAATTGGGTCGTGCATGACGCCGCCGGACTCCATTTTCAACGAATGTGAAGAGAATTTGAACTCCATCTTATCGGAAATTCGTCTGAATCTCTCCACCGGCAAATCGACTCGTCGGCCGATCATCGCCGAAACAGAGCAAGTGAACTTAGAGGCTGGAATGGTGAACCGAAATCGACTCGGGAGAAAAACCCAGTTCGCTTATGTTGCTCTAGCAGAGCCATGGCCGAAAGTTTCTGGTTTTGTGAAGGTCGATCTCTTCACCGGAGAATCCAGAAAGTATCTCTACGGCGAGCAGAGGTACGGCGGCGAGCCTCTGTTTCTCCCTAGAGAAGGTGCAGAGGCGGAGGACGACGGCCACATCTTAACGTTCGTTCACGACGAGAAGGAATGGAAATCAGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTGAAGCTTCCTTCTCGAGTTCCTTATGGCTTCCATGGAACATTCATAAGCTCCAATGATTTACAGAAGCAGAAACAGTAA

Protein sequence

MASLASSSSSSSWVKSGFSSLMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQKQ
Homology
[Close]
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 5.8e-250
Identity = 427/587 (72.74%), Postives = 491/587 (83.65%), Query Frame = 0 

Query: 7   SSSSSSWVKSGFSSLMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVP 66
           SSS SS +    S  M+ R +  ++     V+ +LHTP  +  PK+S     SP     P
Sbjct: 25  SSSQSSDLSYCSSLPMASRVTRKLN-----VSSALHTPPALHFPKQSS---NSPAIVVKP 84

Query: 67  KPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVPE 126
           K       S +++ N  QRAAA ALDA E  LVS E+ H LPKTADPSVQIAGNFAPV E
Sbjct: 85  K----AKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNE 144

Query: 127 QPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYAC 186
           QPVR+ LPV+GK+P+SI+G+YVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  SYAC
Sbjct: 145 QPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSASYAC 204

Query: 187 RFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGL 246
           RFT+T R VQER  GRPVFPKAIGELHGH+GIARL+LFYAR   G+VD +HG GVANAGL
Sbjct: 205 RFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGL 264

Query: 247 VYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALS 306
           VYFNG+LLAMSEDDLPY++++TP GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+FALS
Sbjct: 265 VYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALS 324

Query: 307 YDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIR 366
           YDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FV++PDQQVVFKLPEMIR
Sbjct: 325 YDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIR 384

Query: 367 GGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCM 426
           GGSPV+YDK K +RFG++DK A D++ IKWID PDCFCFHLWNAWEEPET+E+VVIGSCM
Sbjct: 385 GGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCM 444

Query: 427 TPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIA-ETEQVNLEAGMVNRNRLGRKTQ 486
           TPPDSIFNE +ENL S+LSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN LGRKT+
Sbjct: 445 TPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTK 504

Query: 487 FAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFV 546
           FAY+ALAEPWPKVSGF KVDL TGE +K+LYG+ RYGGEPLFLP EG E ED+G+IL FV
Sbjct: 505 FAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGYILCFV 564

Query: 547 HDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
           HDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI ++DL KQ
Sbjct: 565 HDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

[Close]
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 864.0 bits (2231), Expect = 9.9e-250
Identity = 423/602 (70.27%), Postives = 496/602 (82.39%), Query Frame = 0 

Query: 6   SSSSSSSWVKSGFSSLMSERFSVNMDS------GRNR----VACSLHTPSIIQIPKRSQT 65
           +S ++++W+K+  S   S+ F    +S        NR    +  SL  P I+  PK+S  
Sbjct: 5   TSHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSN 64

Query: 66  AFRSPVKTTVPKPVPV---PAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTAD 125
            +++P   T+  P       + S + +WN +Q+AAAMALDAVE AL   E +H LPKTAD
Sbjct: 65  -YQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTAD 124

Query: 126 PSVQIAGNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVH 185
           P VQI+GNFAPVPE PV + LPV GK+P+ ++G+YVRNGANPL EP +GHH FDGDGMVH
Sbjct: 125 PRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVH 184

Query: 186 AVEFTEDGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGL 245
           AV+F ++G  SYACRFTET+RLVQE+A GRPVFPKAIGELHGHSGIARL+LFYARGLFGL
Sbjct: 185 AVQF-KNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGL 244

Query: 246 VDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAH 305
           VDHS G GVANAGLVYFN +LLAMSEDDLPY ++VTP GDLKT GRFDFDGQLKSTMIAH
Sbjct: 245 VDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAH 304

Query: 306 PKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVII 365
           PKLDPVSGE+FALSYDVIQKPYLKYF+FS  GEKS DVEIP+  PTMMHDFAITE FV+I
Sbjct: 305 PKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVI 364

Query: 366 PDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWE 425
           PDQQVVFK+ EMIRGGSPV+YDK K SRFG++DK A D + +KW++VPDCFCFHLWNAWE
Sbjct: 365 PDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWE 424

Query: 426 EPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAE-TEQVNLE 485
           E ET+EIVVIGSCMTPPDSIFNEC+E L S+LSEIRLNL TGKSTR+ II    EQVNLE
Sbjct: 425 EAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLE 484

Query: 486 AGMVNRNRLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPRE 545
           AGMVNRN+LGRKT++AY+A+AEPWPKVSGF KV+LFTGE  K++YG+ +YGGEPLFLPR+
Sbjct: 485 AGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRD 544

Query: 546 -GAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQ 593
             ++ EDDG+IL FVHDEKEWKSELQIVNAM+L+LEATVKLPSRVPYGFHGTFI++NDL 
Sbjct: 545 PNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLA 604

[Close]
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 2.9e-249
Identity = 427/602 (70.93%), Postives = 487/602 (80.90%), Query Frame = 0 

Query: 4   LASSSSSSSWVKSGFSSLMSERFSVNMDSGRNRVACS---LHTPSIIQIPKRSQTAFRSP 63
           LAS+SSS + +           F     S  N + CS   LH P   Q    S T   +P
Sbjct: 25  LASTSSSPTTLLP---------FKKRSSSNTNTITCSLQTLHYPKQYQPTSTSTTTTPTP 84

Query: 64  VK------TTVP----KPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKT 123
           +K      TT P    KP+        ++WNFLQ+AAA  LD VE ALVS E KH LPKT
Sbjct: 85  IKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPKT 144

Query: 124 ADPSVQIAGNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGM 183
           ADP VQIAGNFAPVPE    + LPV+GK+P+ I G+YVRNGANPL+EPV+GHH FDGDGM
Sbjct: 145 ADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDGM 204

Query: 184 VHAVEFTEDGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLF 243
           VHAV+FT +G  SYACRFTETQRL QE++ GRPVFPKAIGELHGHSGIARLLLFYAR LF
Sbjct: 205 VHAVKFT-NGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLF 264

Query: 244 GLVDHSHGIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMI 303
            LVD SHG+GVANAGLVYFN  LLAMSEDDLPY +R+T  GDL TVGR+DF+GQL STMI
Sbjct: 265 QLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMI 324

Query: 304 AHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFV 363
           AHPKLDPV+G++ ALSYDV+QKPYLKYF+FS +G KSPDVEIPL +PTMMHDFAITE FV
Sbjct: 325 AHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFV 384

Query: 364 IIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNA 423
           ++PDQQVVFKL EMI GGSPV+YDK KTSRFG++DKNA DA A++WID P+CFCFHLWNA
Sbjct: 385 VVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNA 444

Query: 424 WEEPETEEIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNL 483
           WEEPET+EIVVIGSCMTP DSIFNEC+E+L S+LSEIRLNL TGKSTRRPII++ EQVNL
Sbjct: 445 WEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVNL 504

Query: 484 EAGMVNRNRLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPR 543
           EAGMVNRN+LGRKTQFAY+ALAEPWPKVSGF KVDLF+GE +KY+YGE+++GGEPLFLP 
Sbjct: 505 EAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFLP- 564

Query: 544 EGAEAEDDGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQ 593
              E E DG+IL FVHDEKEWKSELQIVNA  L+LEA++KLPSRVPYGFHGTFI S DL+
Sbjct: 565 -NGEEEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRVPYGFHGTFIHSKDLR 614

[Close]
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 2.4e-243
Identity = 410/571 (71.80%), Postives = 473/571 (82.84%), Query Frame = 0 

Query: 23  SERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVPKPVPVPAPSVSEEWNF 82
           S  +S++       ++   HT +  +I     T    P +   PK  P      +  WNF
Sbjct: 17  STSYSLDFTLPSKSISMKNHTTTKTKIHSALLTL---PKQNNTPKNQP---QFQTSHWNF 76

Query: 83  LQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVPEQPVRKGLPVIGKVPES 142
            Q+AAA ALD VE ALVS E ++ LPKTADP VQIAGNFAPVPEQ VR  LPV G +P+ 
Sbjct: 77  FQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDC 136

Query: 143 IRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYACRFTETQRLVQERAYGR 202
           I G+YVRNGANPL EPV+GHHLFDGDGMVHAV   E+G VSY+CRFTET+RLVQER  G 
Sbjct: 137 INGVYVRNGANPLFEPVAGHHLFDGDGMVHAVT-VENGSVSYSCRFTETERLVQERELGH 196

Query: 203 PVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGLVYFNGQLLAMSEDDLP 262
           PVFPKAIGELHGHSGIARLLLFYARG+FGLVDHSHG GVANAGLV+FN +LLAMSEDD+P
Sbjct: 197 PVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVP 256

Query: 263 YEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSP 322
           Y ++V P+GDL+TVGR++FD QLKSTMIAHPK+DPVSGE+FALSYDV+QKPYLK FKFSP
Sbjct: 257 YHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSP 316

Query: 323 EGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIRGGSPVIYDKEKTSRFG 382
           +GEKSPDVEIPL  PTMMHDFAITE +V+IPDQQVVFKL EMI+GGSPVIYDK K SRFG
Sbjct: 317 DGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFG 376

Query: 383 VMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCMTPPDSIFNECEENLNS 442
           ++ KNA ++  I W++  + FCFHLWNAWEEPET+E++VIGSCMTPPDSIFNEC ENL S
Sbjct: 377 ILPKNAENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNENLKS 436

Query: 443 ILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRKTQFAYVALAEPWPKVSGFV 502
           +LSEIRLNL TG+STRR +++ ++QVNLEAGMVNRN+LGRKTQFAY+A+AEPWPKVSGF 
Sbjct: 437 VLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFA 496

Query: 503 KVDLFTGESRKYLYGEQRYGGEPLFLPRE-GAEAEDDGHILTFVHDEKEWKSELQIVNAM 562
           KVDL TGE +K++YG++RYGGEPLFLPR   +E EDDG+IL F HDEK WKSELQIVNAM
Sbjct: 497 KVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTWKSELQIVNAM 556

Query: 563 TLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
           TLELEATVKLPSRVPYGFHGTFISS DLQ Q
Sbjct: 557 TLELEATVKLPSRVPYGFHGTFISSKDLQNQ 580

[Close]
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 3.8e-233
Identity = 409/595 (68.74%), Postives = 483/595 (81.18%), Query Frame = 0 

Query: 6   SSSSSSSWVKSGFSSLMSERFSVNMDSGRNRVAC----SLHTPSIIQIPKRSQTAFRSPV 65
           S+SSSSS  K     L S  FS  + + +  V C    S++  S +    +S T F+ P 
Sbjct: 70  STSSSSSRPK-----LQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPS 129

Query: 66  KTTVPKPVPVPAPSVSEE---WNFLQRAAAMALDAVEDALVSVE-RKHSLPKTADPSVQI 125
              +   V    P  + +    N +QR AAM LDAVE+A++S E R+H  PKTADP+VQI
Sbjct: 130 WKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQI 189

Query: 126 AGNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFT 185
           AGNF PVPE+PV   LPV G VPE I+G+YVRNGANPLH+PVSGHHLFDGDGMVHAV F 
Sbjct: 190 AGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF- 249

Query: 186 EDGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSH 245
           ++G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + 
Sbjct: 250 DNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTG 309

Query: 246 GIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDP 305
           G+GVANAGLVYFNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP
Sbjct: 310 GLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDP 369

Query: 306 VSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQV 365
            + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FV+IPDQQV
Sbjct: 370 ETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQV 429

Query: 366 VFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETE 425
           VF+LPEMIRGGSPV+YD++K SRFG+++KNA DA++I+WI+VPDCFCFHLWN+WEEPET+
Sbjct: 430 VFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETD 489

Query: 426 EIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNR 485
           E+VVIGSCMTPPDSIFNE +E L S+LSEIRLNL TG+STRRP+I  +EQVNLEAGMVNR
Sbjct: 490 EVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNR 549

Query: 486 NRLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAED 545
           N LGRKT++AY+AL EPWPKVSGF KVDL TGE RKY+YGE +YGGEPLFLP  G   ED
Sbjct: 550 NLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEED 609

Query: 546 DGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
            G+I+ FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS  DL KQ
Sbjct: 610 GGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

[Close]
BLAST of CmaCh16G004600 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 864.8 bits (2233), Expect = 4.1e-251
Identity = 427/587 (72.74%), Postives = 491/587 (83.65%), Query Frame = 0 

Query: 7   SSSSSSWVKSGFSSLMSERFSVNMDSGRNRVACSLHTPSIIQIPKRSQTAFRSPVKTTVP 66
           SSS SS +    S  M+ R +  ++     V+ +LHTP  +  PK+S     SP     P
Sbjct: 25  SSSQSSDLSYCSSLPMASRVTRKLN-----VSSALHTPPALHFPKQSS---NSPAIVVKP 84

Query: 67  KPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVPE 126
           K       S +++ N  QRAAA ALDA E  LVS E+ H LPKTADPSVQIAGNFAPV E
Sbjct: 85  K----AKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNE 144

Query: 127 QPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYAC 186
           QPVR+ LPV+GK+P+SI+G+YVRNGANPLHEPV+GHH FDGDGMVHAV+F E G  SYAC
Sbjct: 145 QPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSASYAC 204

Query: 187 RFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAGL 246
           RFT+T R VQER  GRPVFPKAIGELHGH+GIARL+LFYAR   G+VD +HG GVANAGL
Sbjct: 205 RFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVANAGL 264

Query: 247 VYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALS 306
           VYFNG+LLAMSEDDLPY++++TP GDLKTVGRFDFDGQL+STMIAHPK+DP SGE+FALS
Sbjct: 265 VYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALS 324

Query: 307 YDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMIR 366
           YDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FV++PDQQVVFKLPEMIR
Sbjct: 325 YDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIR 384

Query: 367 GGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSCM 426
           GGSPV+YDK K +RFG++DK A D++ IKWID PDCFCFHLWNAWEEPET+E+VVIGSCM
Sbjct: 385 GGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCM 444

Query: 427 TPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIA-ETEQVNLEAGMVNRNRLGRKTQ 486
           TPPDSIFNE +ENL S+LSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN LGRKT+
Sbjct: 445 TPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTK 504

Query: 487 FAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGHILTFV 546
           FAY+ALAEPWPKVSGF KVDL TGE +K+LYG+ RYGGEPLFLP EG E ED+G+IL FV
Sbjct: 505 FAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGYILCFV 564

Query: 547 HDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
           HDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI ++DL KQ
Sbjct: 565 HDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQ 597

[Close]
BLAST of CmaCh16G004600 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 808.9 bits (2088), Expect = 2.7e-234
Identity = 409/595 (68.74%), Postives = 483/595 (81.18%), Query Frame = 0 

Query: 6   SSSSSSSWVKSGFSSLMSERFSVNMDSGRNRVAC----SLHTPSIIQIPKRSQTAFRSPV 65
           S+SSSSS  K     L S  FS  + + +  V C    S++  S +    +S T F+ P 
Sbjct: 70  STSSSSSRPK-----LQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPS 129

Query: 66  KTTVPKPVPVPAPSVSEE---WNFLQRAAAMALDAVEDALVSVE-RKHSLPKTADPSVQI 125
              +   V    P  + +    N +QR AAM LDAVE+A++S E R+H  PKTADP+VQI
Sbjct: 130 WKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQI 189

Query: 126 AGNFAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFT 185
           AGNF PVPE+PV   LPV G VPE I+G+YVRNGANPLH+PVSGHHLFDGDGMVHAV F 
Sbjct: 190 AGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF- 249

Query: 186 EDGEVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSH 245
           ++G VSYACRFTET RLVQER  GRPVFPKAIGELHGH GIA+L+LF  RGLFGLVD + 
Sbjct: 250 DNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTG 309

Query: 246 GIGVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDP 305
           G+GVANAGLVYFNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQLKSTMIAHPK+DP
Sbjct: 310 GLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDP 369

Query: 306 VSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQV 365
            + E+FALSYDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FV+IPDQQV
Sbjct: 370 ETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQV 429

Query: 366 VFKLPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETE 425
           VF+LPEMIRGGSPV+YD++K SRFG+++KNA DA++I+WI+VPDCFCFHLWN+WEEPET+
Sbjct: 430 VFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETD 489

Query: 426 EIVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNR 485
           E+VVIGSCMTPPDSIFNE +E L S+LSEIRLNL TG+STRRP+I  +EQVNLEAGMVNR
Sbjct: 490 EVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNR 549

Query: 486 NRLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAED 545
           N LGRKT++AY+AL EPWPKVSGF KVDL TGE RKY+YGE +YGGEPLFLP  G   ED
Sbjct: 550 NLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEED 609

Query: 546 DGHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
            G+I+ FVHDE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS  DL KQ
Sbjct: 610 GGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

[Close]
BLAST of CmaCh16G004600 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.5e-229
Identity = 375/528 (71.02%), Postives = 443/528 (83.90%), Query Frame = 0 

Query: 66  PKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVP 125
           P P P+     +  WN LQRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APVP
Sbjct: 62  PSPNPIIPEKDTSRWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVP 121

Query: 126 EQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYA 185
           EQ V+  L V GK+P+ I G+Y+RNGANPL EPVSGHHLFDGDGMVHAV+ T +G+ SY+
Sbjct: 122 EQSVKSSLSVDGKIPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYS 181

Query: 186 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 245
           CRFTET+RLVQE+  G P+FPKAIGELHGHSGIARL+LFYARGLFGL++H +G GVANAG
Sbjct: 182 CRFTETERLVQEKQLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAG 241

Query: 246 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 305
           LVYF+ +LLAMSEDDLPY++RVT  GDL+T+GRFDFDGQL S MIAHPK+DPV+ E+FAL
Sbjct: 242 LVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFAL 301

Query: 306 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMI 365
           SYDV++KPYLKYFKFSPEGEKSPDVEIPL  PTMMHDFAITE FV+IPDQQVVFKL +M 
Sbjct: 302 SYDVVKKPYLKYFKFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMF 361

Query: 366 RGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSC 425
            G SPV YD EK SRFG++ +NA DA+ + W++ P+ FCFHLWNAWE PET+E+VVIGSC
Sbjct: 362 LGKSPVKYDGEKISRFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSC 421

Query: 426 MTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRKTQ 485
           MTP DSIFNEC+E LNS+LSEIRLNL TGKSTRR II  + Q+NLEAGMVNRN LGRKT+
Sbjct: 422 MTPADSIFNECDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTR 481

Query: 486 FAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPRE-GAEAEDDGHILTF 545
           +AY+A+AEPWPKVSGF KVDL TGE + + YG ++YGGEP FLPR   ++ EDDG+I++F
Sbjct: 482 YAYLAIAEPWPKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSF 541

Query: 546 VHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
           VHDE+ W+SEL IVNA+TLELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 542 VHDEESWESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

[Close]
BLAST of CmaCh16G004600 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 754.2 bits (1946), Expect = 7.9e-218
Identity = 359/530 (67.74%), Postives = 435/530 (82.08%), Query Frame = 0 

Query: 66  PKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGNFAPVP 125
           P+    P        N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PVP
Sbjct: 55  PRTTATPPQHNPLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVP 114

Query: 126 EQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEVSYA 185
           E  VR+ L V G +P+ I G+Y+RNGANP+ EP +GHHLFDGDGMVHAV+ T +G  SYA
Sbjct: 115 ESSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKIT-NGSASYA 174

Query: 186 CRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVANAG 245
           CRFT+T+RLVQE+  GRPVFPKAIGELHGHSGIARL+LFYARGL GL+++ +G+GVANAG
Sbjct: 175 CRFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAG 234

Query: 246 LVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFAL 305
           LVYFN +LLAMSEDDLPY++++T  GDL+TVGR+DFDGQLKS MIAHPKLDPV+ E+ AL
Sbjct: 235 LVYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHAL 294

Query: 306 SYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLPEMI 365
           SYDV++KPYLKYF+FSP+G KSP++EIPL  PTM+HDFAITE FV+IPDQQVVFKL EMI
Sbjct: 295 SYDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMI 354

Query: 366 RGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIVVIGSC 425
            G SPV++D EK SR G+M K+AT+A+ I W++ P+ FCFHLWNAWE PETEEIVVIGSC
Sbjct: 355 SGKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSC 414

Query: 426 MTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRLGRKTQ 485
           M+P DSIFNE +E+L S+LSEIR+NL T K+TRR ++   E VNLE GMVNRNRLGRKT+
Sbjct: 415 MSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLV-NEDVNLEIGMVNRNRLGRKTR 474

Query: 486 FAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLP---REGAEAEDDGHIL 545
           FA++A+A PWPKVSGF KVDL TGE +KY+YG ++YGGEP FLP     G E EDDG+I 
Sbjct: 475 FAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIF 534

Query: 546 TFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
             VHDE+   SELQI+NA+ L+LEAT+KLPSRVPYGFHGTF+ SN+L  Q
Sbjct: 535 CHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582

[Close]
BLAST of CmaCh16G004600 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 643.7 bits (1659), Expect = 1.5e-184
Identity = 309/534 (57.87%), Postives = 402/534 (75.28%), Query Frame = 0 

Query: 64  TVPKPVPVPAPSVSEEWNFLQRAAAMALDAVEDALV-SVERKHSLPKTADPSVQIAGNFA 123
           T P P PV         N LQ+ AA  LD +E ++V  +E+   LPK  DP+VQ++GNFA
Sbjct: 47  TSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFA 106

Query: 124 PVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDGEV 183
           PV E PV+ GL V+G++P  ++G+Y+RNGANP+  P++GHHLFDGDGM+HAV    D +V
Sbjct: 107 PVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQV 166

Query: 184 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIGVA 243
           SY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GLVD + G+GVA
Sbjct: 167 SYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVA 226

Query: 244 NAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 303
           NAG+V+FNG+LLAMSEDDLPY++++   GDL+T+GRF FD Q+ S++IAHPK+D  +G++
Sbjct: 227 NAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDL 286

Query: 304 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFKLP 363
             LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FV+IPDQQ+VFKL 
Sbjct: 287 HTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLS 346

Query: 364 EMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEE---- 423
           EMIRGGSPVIY KEK +RFGV+ K     + I W+DVPDCFCFHLWNAWEE  TEE    
Sbjct: 347 EMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEE-RTEEGDPV 406

Query: 424 IVVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRN 483
           IVVIGSCM+PPD+IF+E  E     LSEIRLN+ T +S R+ I+     VNLEAG +NR+
Sbjct: 407 IVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINRS 466

Query: 484 RLGRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDD 543
            +GRK+QF Y+A+A+PWPK SG  KVD+  G   ++ YG  R+GGEP F+P EG   ED 
Sbjct: 467 YVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVP-EGEGEEDK 526

Query: 544 GHILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 593
           G+++ FV DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S N L++Q
Sbjct: 527 GYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575

The following BLAST results are available for this feature:
BLAST of CmaCh16G004600 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LRR75.8e-25072.749-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
O240239.9e-25070.279-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9M6E82.9e-24970.939-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
K4CJJ12.4e-24371.809-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9M9F53.8e-23368.749-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O... [more]
BLAST of CmaCh16G004600 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G14440.14.1e-25172.74nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G78390.12.7e-23468.74nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT1G30100.11.5e-22971.02nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.17.9e-21867.74nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT3G24220.11.5e-18457.87nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 122..584
e-value: 1.3E-117
score: 393.8
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 67..592
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 67..592

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G004600.1CmaCh16G004600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010162 seed dormancy process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0045549 9-cis-epoxycarotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen