CmaCh16G004120 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G004120
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionTranscription elongation factor 1 homolog
LocationCma_Chr16: 2055178 .. 2057384 (-)
RNA-Seq ExpressionCmaCh16G004120
SyntenyCmaCh16G004120
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCCTCCATTCTCCATGCAAGGGTAGAATTAGTTTGAGGTTTTTCTGTAGTTACAGAATTAAGTTGGGGTTATTTTCGTAATTAAATAATGAACGTTTAAATCTCAGATGTCTATAAATGCCACCTCCTCCCTCAATTCCTTGTATCATTCTTCAATTCGGCCGGCGACTCGTCTGCGAAGAGAACGTTAATTCCTCGCAATTCGATCAGCGATCTGGTATGACTTTTTATTCTCTTCTCTCTTTAGGGTTTGTAGTTCTAGGGTTTTATTAAGCCTTCGTCTGGCAGATTATTGTGCCTTTTTCGTAATGCCTGCAACCTAGGGTTCTACTTCTTGTCGTATATTATGGTTTTCTTCCCGTTCTTTTGTAATGTCGGCGTTTTTTTGCGTTTTTTCGTTGCGGGTGGTTATTCTTATTGAATTTTGTTGGTGGCGTGGTGTTGCTCTTATTGAAGATGGGAGACATTGTTTGAGTAACTTGTAGCTATGCTATCTGTGGCGAAAATGGAAATTTTAGTTATTGTTTGCTTTTGGTTATTTTCATAGACGGTGAGTTTCTATGATTCTTTAGGGAAGTGAAACTTGCATCTCTTGTACGATTTTACTTAATTGCGGCCGTCCATGGATTGGGGATGAATTTTTCGGATCAAGAGGATGGGTGATGAATATGAGTTTTTCTGTAGGAGGATTGGTAGCTTGTTGACTGTGCTTGATATGCTTGAAAGATCTAGGCCTGATATCCTTTAAAGTTTGATTACAACGCTCATTTTGAAGACTGATTGATGGTGATATTGTTTATGGATGTGAACAGACTCACGCCACAAGAAATGGGGAAACGAAAGTCAAGTGCAAAGCCCCCACCTAGGAAGAGAATGGACAAGCTTGATACTGTTTTTAGCTGTCCCTTCTGCAATCATGGAACCAGTGTTGAATGTCGCATGTAAGTTCGCAATTTGCATTTGGTTCCGCTTGGGGGGTTCTTGTAACACGTGAGGGAGCGATTTTTTTGAAGGATTTTTATATTTTGTGGCATATGAAAACGCTGGAATAGTGAAAATGTATAATACCAAAAACTTGTGTCCTGGATTGGTCTCTCAGTCTAGGTGCCTTATTTGTTTGGCCTTATAACGAGGGTGTTTGTATGGTTTTAGTGACATGAAGAACCTGATTGGGGAAGCCTCCTGTGGGATTTGCCAAGAAAGCTTTAGCACAACTGTTACAGGTATTCTCTTTGAATCTCTTATTCCACGAGTGTTTGTTTTGTTTTCATTATCTGTTGTGTGCGTTTTACTTTATGCTTCATATTATGTTGTGGCCTGTAATTCAAGCAATTTAGGATTTTTTTAAAGAATATTTTTAATGGGTATTTTTTTGTTCTACTGGAGTAATTTTTTTTTTATCCGAACCTTAAATAGTGCACGGGACTGTGGGATTATTCTAGGGAACCCCATTTTCTCTATGCTCGAAATAGATGGACGAATGGAAGATTTGTTATGCAGTTGAGACTTTTATTCCTGTTGTAGGGAAGTCTATCAGATGAAAATGTGTACCAAGTGTAAGGCATCATGTATGATTTAGGTGGATTTAATTGTTTTCGTGTCGAAGTCTCGGCTTACAACTTGATTCTTATGCACTAATATTTCATTTGTTTTGTTTTGCAGCCTTGACTGAGCCAATAGATATGTAAGTAAAAATTAAACGCAACTGGTGTATGTCGATATTGATGTTCTACAGGGCTTTGGTTGACATGATGTTGTTTTGATCAGATATAGCGAATGGATCGATGAATGTGAGCGGGTCAACAACCCTGAAGAAGACGATGGTGCTTAGAGGGAATCATTGACAATGCTGCATAGAGGGAATCAATCATTGGGGAAGGTTGAAGTTTGCCATTTTTCTATGAGAACGAAGCTTGCTCGTTCAACCGTTCCTTTTACGAATCTTAATTAATAGGGTGACTTACCGGTAGGTATAACAACACTCTTACCCTCGTAGCAGTATAGACAAGCATGTTTGTAATAATAGTATCATACTTTTCCAGAGTTTGTGTGAATTTTCATCTATGTGCAAATGAAGAGTGGGTTTGTGAACCATCCTTTATTATTACTAATAATTCTACCTTGTAGATGAACTATTAACGGACGTTCGTTCCTTCATGCATAAGACTTTTAATATAGTTAATACTGGATTCTCCCTTGATAGT

mRNA sequence

ATGGTCCTCCATTCTCCATGCAAGGCGATCTGACTCACGCCACAAGAAATGGGGAAACGAAAGTCAAGTGCAAAGCCCCCACCTAGGAAGAGAATGGACAAGCTTGATACTGTTTTTAGCTGTCCCTTCTGCAATCATGGAACCAGTGTTGAATGTCGCATTGACATGAAGAACCTGATTGGGGAAGCCTCCTGTGGGATTTGCCAAGAAAGCTTTAGCACAACTGTTACAGCCTTGACTGAGCCAATAGATATATATAGCGAATGGATCGATGAATGTGAGCGGGTCAACAACCCTGAAGAAGACGATGGTGCTTAGAGGGAATCATTGACAATGCTGCATAGAGGGAATCAATCATTGGGGAAGGTTGAAGTTTGCCATTTTTCTATGAGAACGAAGCTTGCTCGTTCAACCGTTCCTTTTACGAATCTTAATTAATAGGGTGACTTACCGGTAGGTATAACAACACTCTTACCCTCGTAGCAGTATAGACAAGCATGTTTGTAATAATAGTATCATACTTTTCCAGAGTTTGTGTGAATTTTCATCTATGTGCAAATGAAGAGTGGGTTTGTGAACCATCCTTTATTATTACTAATAATTCTACCTTGTAGATGAACTATTAACGGACGTTCGTTCCTTCATGCATAAGACTTTTAATATAGTTAATACTGGATTCTCCCTTGATAGT

Coding sequence (CDS)

ATGGGGAAACGAAAGTCAAGTGCAAAGCCCCCACCTAGGAAGAGAATGGACAAGCTTGATACTGTTTTTAGCTGTCCCTTCTGCAATCATGGAACCAGTGTTGAATGTCGCATTGACATGAAGAACCTGATTGGGGAAGCCTCCTGTGGGATTTGCCAAGAAAGCTTTAGCACAACTGTTACAGCCTTGACTGAGCCAATAGATATATATAGCGAATGGATCGATGAATGTGAGCGGGTCAACAACCCTGAAGAAGACGATGGTGCTTAG

Protein sequence

MGKRKSSAKPPPRKRMDKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTTVTALTEPIDIYSEWIDECERVNNPEEDDGA
Homology
[Close]
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match: Q8LHP0 (Transcription elongation factor 1 homolog OS=Oryza sativa subsp. japonica OX=39947 GN=Os07g0631100 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.9e-43
Identity = 81/89 (91.01%), Postives = 86/89 (96.63%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRMDKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTTV 60
          MGKRKS+AKPPP+KRMDKLDTVFSCPFCNHG+SVECRIDMKNLIGEASC ICQE+FSTTV
Sbjct: 1  MGKRKSAAKPPPKKRMDKLDTVFSCPFCNHGSSVECRIDMKNLIGEASCRICQENFSTTV 60

Query: 61 TALTEPIDIYSEWIDECERVNNPEEDDGA 90
           ALTEPIDIYSEWIDECERVNN E+DDGA
Sbjct: 61 NALTEPIDIYSEWIDECERVNNVEDDDGA 89

[Close]
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match: Q8LEF3 (Transcription elongation factor 1 homolog OS=Arabidopsis thaliana OX=3702 GN=At5g46030 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-36
Identity = 70/86 (81.40%), Postives = 78/86 (90.70%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRMDKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTTV 60
          MGKRKS AKP P KRMDKLDT+FSCPFCNHG+SVEC IDMK+LIG+A+C IC+ESFSTT+
Sbjct: 1  MGKRKSRAKPAPTKRMDKLDTIFSCPFCNHGSSVECIIDMKHLIGKAACRICEESFSTTI 60

Query: 61 TALTEPIDIYSEWIDECERVNNPEED 87
          TALTE IDIYSEWIDECERVN  E+D
Sbjct: 61 TALTEAIDIYSEWIDECERVNTAEDD 86

[Close]
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match: P36053 (Transcription elongation factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ELF1 PE=1 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.0e-20
Identity = 45/91 (49.45%), Postives = 61/91 (67.03%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRMDKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTTV 60
          MGKRK S + P ++ + KLDT F+C FCNH  SV C +D KN IG  SC IC +SF T +
Sbjct: 1  MGKRKKSTRKPTKRLVQKLDTKFNCLFCNHEKSVSCTLDKKNSIGTLSCKICGQSFQTRI 60

Query: 61 TALTEPIDIYSEWIDECERVNN---PEEDDG 89
           +L++P+D+YS+W D  E VN+    + DDG
Sbjct: 61 NSLSQPVDVYSDWFDAVEEVNSGRGSDTDDG 91

[Close]
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match: A4IFR3 (Transcription elongation factor 1 homolog OS=Bos taurus OX=9913 GN=ELOF1 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-20
Identity = 42/82 (51.22%), Postives = 56/82 (68.29%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRM-DKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTT 60
          MG+RKS  KPPP+K+M   L+T F+CPFCNH  S + ++D     G  SC +C E F T 
Sbjct: 1  MGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTP 60

Query: 61 VTALTEPIDIYSEWIDECERVN 82
          +T L+EP+D+YS+WID CE  N
Sbjct: 61 ITYLSEPVDVYSDWIDACEAAN 82

[Close]
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match: P60002 (Transcription elongation factor 1 homolog OS=Homo sapiens OX=9606 GN=ELOF1 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 4.9e-20
Identity = 42/82 (51.22%), Postives = 56/82 (68.29%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRM-DKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTT 60
          MG+RKS  KPPP+K+M   L+T F+CPFCNH  S + ++D     G  SC +C E F T 
Sbjct: 1  MGRRKSKRKPPPKKKMTGTLETQFTCPFCNHEKSCDVKMDRARNTGVISCTVCLEEFQTP 60

Query: 61 VTALTEPIDIYSEWIDECERVN 82
          +T L+EP+D+YS+WID CE  N
Sbjct: 61 ITYLSEPVDVYSDWIDACEAAN 82

[Close]
BLAST of CmaCh16G004120 vs. TAIR 10
Match: AT5G46030.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF701, zinc-binding putative (InterPro:IPR007808); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 153.3 bits (386), Expect = 9.2e-38
Identity = 70/86 (81.40%), Postives = 78/86 (90.70%), Query Frame = 0 

Query: 1  MGKRKSSAKPPPRKRMDKLDTVFSCPFCNHGTSVECRIDMKNLIGEASCGICQESFSTTV 60
          MGKRKS AKP P KRMDKLDT+FSCPFCNHG+SVEC IDMK+LIG+A+C IC+ESFSTT+
Sbjct: 1  MGKRKSRAKPAPTKRMDKLDTIFSCPFCNHGSSVECIIDMKHLIGKAACRICEESFSTTI 60

Query: 61 TALTEPIDIYSEWIDECERVNNPEED 87
          TALTE IDIYSEWIDECERVN  E+D
Sbjct: 61 TALTEAIDIYSEWIDECERVNTAEDD 86

The following BLAST results are available for this feature:
BLAST of CmaCh16G004120 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LHP01.9e-4391.01Transcription elongation factor 1 homolog OS=Oryza sativa subsp. japonica OX=399... [more]
Q8LEF31.3e-3681.40Transcription elongation factor 1 homolog OS=Arabidopsis thaliana OX=3702 GN=At5... [more]
P360531.0e-2049.45Transcription elongation factor 1 OS=Saccharomyces cerevisiae (strain ATCC 20450... [more]
A4IFR34.9e-2051.22Transcription elongation factor 1 homolog OS=Bos taurus OX=9913 GN=ELOF1 PE=3 SV... [more]
P600024.9e-2051.22Transcription elongation factor 1 homolog OS=Homo sapiens OX=9606 GN=ELOF1 PE=1 ... [more]
BLAST of CmaCh16G004120 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G46030.19.2e-3881.40unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF701,... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007808Transcription elongation factor 1PFAMPF05129Elf1coord: 2..78
e-value: 6.4E-31
score: 106.2
IPR007808Transcription elongation factor 1PANTHERPTHR20934UNCHARACTERIZEDcoord: 1..89
IPR038567Transcription elongation factor 1 superfamilyGENE3D2.20.25.190coord: 16..82
e-value: 9.2E-30
score: 104.6
NoneNo IPR availableSUPERFAMILY57783Zinc beta-ribboncoord: 1..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G004120.1CmaCh16G004120.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006368 transcription elongation from RNA polymerase II promoter
biological_process GO:0006414 translational elongation
cellular_component GO:0008023 transcription elongation factor complex
molecular_function GO:0046872 metal ion binding
molecular_function GO:0000993 RNA polymerase II complex binding
molecular_function GO:0003746 translation elongation factor activity