CmaCh16G003780 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G003780
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionglutelin type-A 2-like
LocationCma_Chr16: 1794037 .. 1795534 (+)
RNA-Seq ExpressionCmaCh16G003780
SyntenyCmaCh16G003780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCCTTCCATGGAACAACCGATGAATCCAAAGCCCTTCACTGAGGTAGAAGCTGGATCTTATCACAAATGGCTGCCTTCTGAATATCCTTTGCTTGCTCATAACAAAGTTGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTGGTCCCCCACTACGCCGATTGTTCTAAAGTGGGCTATGTTCTTCAAGGTAGTTAATCTTTTTACTGAGTTGTTGTATGATTTTGTCGACTTTGAACATATGCTATCATGATTGTATTTGGATTCTCTTCTAATAATTTCCGACATATTTTGGTTGCAGGGGAGAATGGAGTTGCCGGGTTGGTGTTTCCGAGCAAGTCCGATGAGGTGGTGGTGAACTTGAAGAAAGGAGATTTGATTCCGGTGCCGAACGGAGTCTCATCGTGGTGGTTCAACGATGGCGACTCCGATTTGGAAATCATATTTTTGGGTGAATCCAAAAACGCTCATGTTCCTGGTGACATCTCTTACTTTGTTCTCTCCGGCATTCTCAGCCTCCTGAATGGCTTCTCGCCGGAGTACGTCGGAGAAACCTATTCTCTAAACGGAGAAGAAACAACCCAATTTCTCAAAAGTCAATCCAACGCTCTGATTTTCTCAATTCAGCAAACCCAATCCCTTCCCAAGCCACCCAAATACAGTAAATTTGTCTACAACATTGACGCCGCCGCGCCTGACGGTAGAGTCAAGGGCGGCGCTGGCGCTGTCACGACGGTGACGGAATCAAAATTTCCGTTTATTGGTCAATCTGGGTTGACGGCGATTCTCGAAAAGCTCGACGCCAACGCCGTCCGTTCGCCGGTGTACGTCGCGGAGCCGTACGATCAACTGATCTACGTGGCTAAAGGACGTGGGAAAATCCAGATCGTTGGATTTTCGAGTAAAATTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTAGTCCCCAAATTCTTCGCCGTCGGAAAAATCGCCGGAGAAGATGGCTTGGAGTGCATCTCCATTATCACAGCTACACAGTAATAAGCTTCAATTTTTATATATATATTTTTTAATTTTAATTTTAATCTCTGAATCTTAAAACTTGACGTTTTAAAATTGAACAGTCCCGTGGTGGAAGAACTGGCCGGAAAGACGTCGGTGTTGGAGGCATTGTCGCCGGAGGTTTTTCAAGTTTCGTTCAATGTGACGGCCGAGTTCGAGAAGCTTCTTAGATCGAAGATCACTAACGCTTCACCGGTGATCCGATCTTCCGATTGAAGAGGGAATTATTAATTAATTACAATAATATTTCAATGTTTGGGTATTATTATTATTATTATTATTATTATTATGGGCTTTTACGCTATTAAATAAATTTGATTTCCCAATCCCAATGATAGTGATGTGGGGGCTTTCTGGTCATTTCACCACTTGAACTTTTATATGAATTATTGTGAAGGATTATGATTGTTGATGAATTGTTATTGTATTGTGAAGGATTATTTTTTTG

mRNA sequence

ATCCCTTCCATGGAACAACCGATGAATCCAAAGCCCTTCACTGAGGTAGAAGCTGGATCTTATCACAAATGGCTGCCTTCTGAATATCCTTTGCTTGCTCATAACAAAGTTGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTGGTCCCCCACTACGCCGATTGTTCTAAAGTGGGCTATGTTCTTCAAGGGGAGAATGGAGTTGCCGGGTTGGTGTTTCCGAGCAAGTCCGATGAGGTGGTGGTGAACTTGAAGAAAGGAGATTTGATTCCGGTGCCGAACGGAGTCTCATCGTGGTGGTTCAACGATGGCGACTCCGATTTGGAAATCATATTTTTGGGTGAATCCAAAAACGCTCATGTTCCTGGTGACATCTCTTACTTTGTTCTCTCCGGCATTCTCAGCCTCCTGAATGGCTTCTCGCCGGAGTACGTCGGAGAAACCTATTCTCTAAACGGAGAAGAAACAACCCAATTTCTCAAAAGTCAATCCAACGCTCTGATTTTCTCAATTCAGCAAACCCAATCCCTTCCCAAGCCACCCAAATACAGTAAATTTGTCTACAACATTGACGCCGCCGCGCCTGACGGTAGAGTCAAGGGCGGCGCTGGCGCTGTCACGACGGTGACGGAATCAAAATTTCCGTTTATTGGTCAATCTGGGTTGACGGCGATTCTCGAAAAGCTCGACGCCAACGCCGTCCGTTCGCCGGTGTACGTCGCGGAGCCGTACGATCAACTGATCTACGTGGCTAAAGGACGTGGGAAAATCCAGATCGTTGGATTTTCGAGTAAAATTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTAGTCCCCAAATTCTTCGCCGTCGGAAAAATCGCCGGAGAAGATGGCTTGGAGTGCATCTCCATTATCACAGCTACACATCCCGTGGTGGAAGAACTGGCCGGAAAGACGTCGGTGTTGGAGGCATTGTCGCCGGAGGTTTTTCAAGTTTCGTTCAATGTGACGGCCGAGTTCGAGAAGCTTCTTAGATCGAAGATCACTAACGCTTCACCGGTGATCCGATCTTCCGATTGAAGAGGGAATTATTAATTAATTACAATAATATTTCAATGTTTGGGTATTATTATTATTATTATTATTATTATTATGGGCTTTTACGCTATTAAATAAATTTGATTTCCCAATCCCAATGATAGTGATGTGGGGGCTTTCTGGTCATTTCACCACTTGAACTTTTATATGAATTATTGTGAAGGATTATGATTGTTGATGAATTGTTATTGTATTGTGAAGGATTATTTTTTTG

Coding sequence (CDS)

ATGGAACAACCGATGAATCCAAAGCCCTTCACTGAGGTAGAAGCTGGATCTTATCACAAATGGCTGCCTTCTGAATATCCTTTGCTTGCTCATAACAAAGTTGCCGCCGGCCGCCTTCTCCTCCGCCCTCGCGGCTTCGTGGTCCCCCACTACGCCGATTGTTCTAAAGTGGGCTATGTTCTTCAAGGGGAGAATGGAGTTGCCGGGTTGGTGTTTCCGAGCAAGTCCGATGAGGTGGTGGTGAACTTGAAGAAAGGAGATTTGATTCCGGTGCCGAACGGAGTCTCATCGTGGTGGTTCAACGATGGCGACTCCGATTTGGAAATCATATTTTTGGGTGAATCCAAAAACGCTCATGTTCCTGGTGACATCTCTTACTTTGTTCTCTCCGGCATTCTCAGCCTCCTGAATGGCTTCTCGCCGGAGTACGTCGGAGAAACCTATTCTCTAAACGGAGAAGAAACAACCCAATTTCTCAAAAGTCAATCCAACGCTCTGATTTTCTCAATTCAGCAAACCCAATCCCTTCCCAAGCCACCCAAATACAGTAAATTTGTCTACAACATTGACGCCGCCGCGCCTGACGGTAGAGTCAAGGGCGGCGCTGGCGCTGTCACGACGGTGACGGAATCAAAATTTCCGTTTATTGGTCAATCTGGGTTGACGGCGATTCTCGAAAAGCTCGACGCCAACGCCGTCCGTTCGCCGGTGTACGTCGCGGAGCCGTACGATCAACTGATCTACGTGGCTAAAGGACGTGGGAAAATCCAGATCGTTGGATTTTCGAGTAAAATTGATGCAGAGGTGAAAATGGGTCAGCTGATTTTAGTCCCCAAATTCTTCGCCGTCGGAAAAATCGCCGGAGAAGATGGCTTGGAGTGCATCTCCATTATCACAGCTACACATCCCGTGGTGGAAGAACTGGCCGGAAAGACGTCGGTGTTGGAGGCATTGTCGCCGGAGGTTTTTCAAGTTTCGTTCAATGTGACGGCCGAGTTCGAGAAGCTTCTTAGATCGAAGATCACTAACGCTTCACCGGTGATCCGATCTTCCGATTGA

Protein sequence

MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD
Homology
BLAST of CmaCh16G003780 vs. ExPASy Swiss-Prot
Match: Q9ZWA9 (12S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 2.2e-23
Identity = 103/393 (26.21%), Postives = 160/393 (40.71%), Query Frame = 0

Query: 7   PKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGENG 66
           P   T+ EAG    W     P L    V   R+ L+P    +P +     + YV+QGE G
Sbjct: 46  PAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGE-G 105

Query: 67  VAGLV---FPSKSDEV----------------------VVNLKKGDLIPVPNGVSSWWFN 126
           V G +    P    EV                      + N ++GD+     GVS WW+N
Sbjct: 106 VMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYN 165

Query: 127 DGDSDLEIIFLGESKNAHVPGD--ISYFVLSGIL--------------SLLNGFSPEYVG 186
            GDSD  I+ + +  N     D     F L+G                +  +GF P  + 
Sbjct: 166 RGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIA 225

Query: 187 ETYSLNGEETTQFLKSQSNA--LIFSIQQTQSLPKPPK----------------YSKFVY 246
           E + +N E   Q    + N   +I +      +  PP+                 +K   
Sbjct: 226 EAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHE 285

Query: 247 NIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLI 306
           NID           AG ++T+     P +    L A+   L +  +  P + A  +  ++
Sbjct: 286 NIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAH-TVL 345

Query: 307 YVAKGRGKIQIV--GFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVV 339
           YV  G+ KIQ+V     S  + +V  GQ+I++P+ FAV K AGE G E IS  T  +  +
Sbjct: 346 YVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYI 405

BLAST of CmaCh16G003780 vs. ExPASy Swiss-Prot
Match: P14323 (Glutelin type-B 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GluB1-A PE=2 SV=3)

HSP 1 Score: 103.6 bits (257), Expect = 4.7e-21
Identity = 90/387 (23.26%), Postives = 165/387 (42.64%), Query Frame = 0

Query: 38  RLLLRPRGFVVPHYADCSKVGYVLQGENGVAGLVFPS----------------------- 97
           R +++P+G +VP Y +   V Y++QG  G  GL FP                        
Sbjct: 85  RRVIQPQGLLVPRYTNIPGVVYIIQG-RGSMGLTFPGCPATYQQQFQQFSSQGQSQSQKF 144

Query: 98  -KSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKN--AHVPGDISYFVLSG 157
               + +   ++GD++ +P GV+ W++NDGD+ +  +++ +  N    +      F+L+G
Sbjct: 145 RDEHQKIHQFRQGDIVALPAGVAHWFYNDGDAPIVAVYVYDVNNNANQLEPRQKEFLLAG 204

Query: 158 I-------------------LSLLNGFSPEYVGETYSLNGEETTQFLKSQSN--ALIFSI 217
                                ++ +GF  E + E   +N     + L+SQ++    I  +
Sbjct: 205 NNNRAQQQQVYGSSIEQHSGQNIFSGFGVEMLSEALGINAVAAKR-LQSQNDQRGEIIHV 264

Query: 218 QQTQSLPKP------------PKYSKFVY------------------------NIDAAAP 277
           +    L KP             +Y +  Y                        NI+  + 
Sbjct: 265 KNGLQLLKPTLTQQQEQAQAQDQYQQVQYSERQQTSSRWNGLEENFCTIKVRVNIENPSR 324

Query: 278 DGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLIYVAKGRG 337
                  AG +T+V   KFP +    ++A    L  NA+ SP +    +  L+Y+ +GR 
Sbjct: 325 ADSYNPRAGRITSVNSQKFPILNLIQMSATRVNLYQNAILSPFWNVNAH-SLVYMIQGRS 384

Query: 338 KIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVVEELAGKT 340
           ++Q+V    K   D  ++ GQL+++P+ +AV K A  +G + I+I T  +  V  LAGK 
Sbjct: 385 RVQVVSNFGKTVFDGVLRPGQLLIIPQHYAVLKKAEREGCQYIAIKTNANAFVSHLAGKN 444

BLAST of CmaCh16G003780 vs. ExPASy Swiss-Prot
Match: Q02897 (Glutelin type-B 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB2 PE=2 SV=2)

HSP 1 Score: 103.6 bits (257), Expect = 4.7e-21
Identity = 88/382 (23.04%), Postives = 166/382 (43.46%), Query Frame = 0

Query: 38  RLLLRPRGFVVPHYADCSKVGYVLQGENGVAGLVFPS----------------------- 97
           R +++P+G +VP Y++   + Y++QG  G  GL FP                        
Sbjct: 85  RRVIQPQGLLVPRYSNTPGLVYIIQG-RGSMGLTFPGCPATYQQQFQQFSSQGQSQSQKF 144

Query: 98  -KSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNA--HVPGDISYFVLSG 157
               + +   ++GD++ +P GV+ W++NDGD+ +  I++ +  N+   +      F+L+G
Sbjct: 145 RDEHQKIHQFRQGDVVALPAGVAHWFYNDGDASVVAIYVYDINNSANQLEPRQKEFLLAG 204

Query: 158 -----------------ILSLLNGFSPEYVGETYSLN--------------GE-----ET 217
                              ++ NGF  E + E   +N              GE       
Sbjct: 205 NNNRVQQVYGSSIEQHSSQNIFNGFGTELLSEALGINTVAAKRLQSQNDQRGEIVHVKNG 264

Query: 218 TQFLK-------SQSNALIFSIQQTQSLPKPPKYS---------KFVYNIDAAAPDGRVK 277
            Q LK        Q+ A    +Q ++      +++         K   NI+  +      
Sbjct: 265 LQLLKPTLTQQQEQAQAQYQEVQYSEQQQTSSRWNGLEENFCTIKARVNIENPSRADSYN 324

Query: 278 GGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLIYVAKGRGKIQIV 337
             AG +++V   KFP +    ++A    L  NA+ SP +    +  L+Y+ +G+ ++Q+V
Sbjct: 325 PRAGRISSVNSQKFPILNLIQMSATRVNLYQNAILSPFWNVNAH-SLVYMIQGQSRVQVV 384

Query: 338 GFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVVEELAGKTSVLEA 340
               K   D  ++ GQL+++P+ +AV K A  +G + I+I T  +  V  LAGK SV  A
Sbjct: 385 SNFGKTVFDGVLRPGQLLIIPQHYAVLKKAEREGCQYIAIKTNANAFVSHLAGKNSVFRA 444

BLAST of CmaCh16G003780 vs. ExPASy Swiss-Prot
Match: P07730 (Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.8e-20
Identity = 86/392 (21.94%), Postives = 164/392 (41.84%), Query Frame = 0

Query: 34  VAAGRLLLRPRGFVVPHYADCSKVGYVLQGENGVAGLVFP-------------------- 93
           V+  R ++ PRG ++PHY + + + Y++QG  G+ G  FP                    
Sbjct: 82  VSVVRRVIEPRGLLLPHYTNGASLVYIIQG-RGITGPTFPGCPETYQQQFQQSGQAQLTE 141

Query: 94  --------SKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPGDIS 153
                       + +   ++GD+I +P GV+ W +NDG+  +  I++ +  N     D  
Sbjct: 142 SQSQSHKFKDEHQKIHRFRQGDVIALPAGVAHWCYNDGEVPVVAIYVTDINNGANQLDPR 201

Query: 154 Y--FVLSG---------------ILSLLNGFSPEYVGETYSLNGEETTQF-LKSQSNALI 213
              F+L+G                 ++ +GFS E + E + ++ +   Q   ++     I
Sbjct: 202 QRDFLLAGNKRNPQAYRREVEEWSQNIFSGFSTELLSEAFGISNQVARQLQCQNDQRGEI 261

Query: 214 FSIQQTQSLPKP--------------------PKYSKFVY-------------------N 273
             +++  SL +P                      Y +  Y                   N
Sbjct: 262 VRVERGLSLLQPYASLQEQEQGQMQSREHYQEGGYQQSQYGSGCPNGLDETFCTMRVRQN 321

Query: 274 IDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLIY 333
           ID           AG VT +    FP +    ++A+   L  NA+ SP +    +  ++Y
Sbjct: 322 IDNPNRADTYNPRAGRVTNLNSQNFPILNLVQMSAVKVNLYQNALLSPFWNINAH-SIVY 381

Query: 334 VAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVVE 339
           + +GR ++Q+V  + K   + E++ GQL++VP+ + V K A  +G   I+  T  + +V 
Sbjct: 382 ITQGRAQVQVVNNNGKTVFNGELRRGQLLIVPQHYVVVKKAQREGCAYIAFKTNPNSMVS 441

BLAST of CmaCh16G003780 vs. ExPASy Swiss-Prot
Match: P15456 (12S seed storage protein CRB OS=Arabidopsis thaliana OX=3702 GN=CRB PE=1 SV=2)

HSP 1 Score: 100.5 bits (249), Expect = 3.9e-20
Identity = 92/414 (22.22%), Postives = 171/414 (41.30%), Query Frame = 0

Query: 5   MNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGE 64
           + P    + E G    W     P L  +  A  R ++ P+G  +P + +  K+ +V+ G 
Sbjct: 38  LEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHG- 97

Query: 65  NGVAGLVFPSKSD------------------------EVVVNLKKGDLIPVPNGVSSWWF 124
            G+ G V P  ++                        + V +L+ GD I  P+GV+ W++
Sbjct: 98  RGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQWFY 157

Query: 125 NDGDSDLEIIFLGE--SKNAHVPGDISYFVLSG----------------ILSLLNGFSPE 184
           N+G+  L ++   +  S    +  ++  F+++G                  ++ NGF+PE
Sbjct: 158 NNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPE 217

Query: 185 YVGETYSLNGEETTQFLKSQSN-ALIFSIQQTQSLPKPPK-------------------- 244
            + + + +N E   Q    Q N   I  +     + +PP                     
Sbjct: 218 ILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETL 277

Query: 245 -YSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 304
              +   N+D  +     K   G ++T+     P +    L+A+   +  NA+  P +  
Sbjct: 278 CTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNV 337

Query: 305 EPYDQLIYVAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISII 348
              +  +YV  G+  IQ+V  + +   D E+  GQL++VP+ F+V K A  +  E I   
Sbjct: 338 NA-NAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFK 397

BLAST of CmaCh16G003780 vs. ExPASy TrEMBL
Match: A0A6J1JDB2 (12S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE=4 SV=1)

HSP 1 Score: 699.9 bits (1805), Expect = 5.4e-198
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP
Sbjct: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA
Sbjct: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

BLAST of CmaCh16G003780 vs. ExPASy TrEMBL
Match: A0A6J1E9P2 (legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.6e-192
Identity = 343/352 (97.44%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLA NKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSG LSLL+GFSPEYVG+TYSLNGEETTQFLKSQSNALIFSIQQTQSLPKP 
Sbjct: 121 PGDISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPS 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKL+ANAVRSPVYVA
Sbjct: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVG SSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPE+FQVSFNVTAEFEKLLRSKITNASPVI SSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of CmaCh16G003780 vs. ExPASy TrEMBL
Match: A0A5A7T7U8 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold84G001330 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.5e-151
Identity = 264/339 (77.88%), Postives = 299/339 (88.20%), Query Frame = 0

Query: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62
           + MNPKPF E E GSY KWLPS+YPLLA   VA GRLLLRPRGF VPHYADCSK GYVLQ
Sbjct: 2   EAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVLQ 61

Query: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122
           GE+GV G VFP+K +EVV+ LKKGDLIPVP+G++SWWFNDGDSDLEIIFLGE+KNAHVPG
Sbjct: 62  GEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVPG 121

Query: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182
           DI+YF+LSG   LL GF+PEYV ++YSL+ EET +FLKSQSN LIF++Q +QSLPKP K+
Sbjct: 122 DITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHKH 181

Query: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242
           SK VYNIDAA PD R K GA AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+AEP
Sbjct: 182 SKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAEP 241

Query: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302
            DQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP++FAVGK+AGE+GLECIS+I ATH
Sbjct: 242 SDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVATH 301

Query: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKI 342
           P+VEELAGKTSVLEALS EVFQVSFNVTAEFEKL RSK+
Sbjct: 302 PMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of CmaCh16G003780 vs. ExPASy TrEMBL
Match: A0A1S3C2D5 (glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 6.5e-151
Identity = 264/339 (77.88%), Postives = 299/339 (88.20%), Query Frame = 0

Query: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62
           + MNPKPF E E GSY KWLPS+YPLLA   VA GRLLLRPRGF VPHYADCSK GYVLQ
Sbjct: 2   EAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVLQ 61

Query: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122
           GE+GV G VFP+K +EVV+ LKKGDLIPVP+G++SWWFNDGDSDLEIIFLGE+KNAHVPG
Sbjct: 62  GEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVPG 121

Query: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182
           DI+YF+LSG   LL GF+PEYV ++YSL+ EET +FLKSQSN LIF++Q +QSLPKP K+
Sbjct: 122 DITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHKH 181

Query: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242
           SK VYNIDAA PD R K GA AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+AEP
Sbjct: 182 SKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAEP 241

Query: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302
            DQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP++FAVGK+AGE+GLECIS+I ATH
Sbjct: 242 SDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVATH 301

Query: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKI 342
           P+VEELAGKTSVLEALS EVFQVSFNVTAEFEKL RSK+
Sbjct: 302 PMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFRSKV 340

BLAST of CmaCh16G003780 vs. ExPASy TrEMBL
Match: A0A5D3BLA4 (Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00380 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.2e-149
Identity = 262/336 (77.98%), Postives = 296/336 (88.10%), Query Frame = 0

Query: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62
           + MNPKPF E E GSY KWLPS+YPLLA   VA GRLLLRPRGF VPHYADCSK GYVLQ
Sbjct: 2   EAMNPKPFFEGEGGSYLKWLPSDYPLLAQTNVAGGRLLLRPRGFAVPHYADCSKFGYVLQ 61

Query: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122
           GE+GV G VFP+K +EVV+ LKKGDLIPVP+G++SWWFNDGDSDLEIIFLGE+KNAHVPG
Sbjct: 62  GEDGVTGFVFPNKCNEVVMKLKKGDLIPVPSGITSWWFNDGDSDLEIIFLGETKNAHVPG 121

Query: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182
           DI+YF+LSG   LL GF+PEYV ++YSL+ EET +FLKSQSN LIF++Q +QSLPKP K+
Sbjct: 122 DITYFILSGPRGLLQGFAPEYVQKSYSLSQEETNKFLKSQSNVLIFTVQPSQSLPKPHKH 181

Query: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242
           SK VYNIDAA PD R K GA AVT VTES FPFIGQ+GLTA+LEKLDANA+RSPVY+AEP
Sbjct: 182 SKLVYNIDAAVPDNRAKVGAAAVTMVTESTFPFIGQTGLTAVLEKLDANAIRSPVYIAEP 241

Query: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302
            DQLIYV KG GKIQ+VGFSSK DA+VK+GQLILVP++FAVGK+AGE+GLECIS+I ATH
Sbjct: 242 SDQLIYVTKGSGKIQVVGFSSKFDADVKIGQLILVPRYFAVGKMAGEEGLECISMIVATH 301

Query: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLR 339
           P+VEELAGKTSVLEALS EVFQVSFNVTAEFEKL R
Sbjct: 302 PMVEELAGKTSVLEALSSEVFQVSFNVTAEFEKLFR 337

BLAST of CmaCh16G003780 vs. NCBI nr
Match: XP_022985328.1 (12S seed storage protein CRD-like [Cucurbita maxima])

HSP 1 Score: 699.9 bits (1805), Expect = 1.1e-197
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP
Sbjct: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA
Sbjct: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 352

BLAST of CmaCh16G003780 vs. NCBI nr
Match: XP_022922755.1 (legumin J-like [Cucurbita moschata])

HSP 1 Score: 679.5 bits (1752), Expect = 1.6e-191
Identity = 343/352 (97.44%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLA NKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAQNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSG LSLL+GFSPEYVG+TYSLNGEETTQFLKSQSNALIFSIQQTQSLPKP 
Sbjct: 121 PGDISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPS 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKL+ANAVRSPVYVA
Sbjct: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLNANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVG SSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPE+FQVSFNVTAEFEKLLRSKITNASPVI SSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of CmaCh16G003780 vs. NCBI nr
Match: KAG6576976.1 (12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 678.3 bits (1749), Expect = 3.5e-191
Identity = 342/352 (97.16%), Postives = 347/352 (98.58%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLA NKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFN+GDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNEGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSG LSLL+GFSPEYVG+TYSLNGEETTQFLKSQSNALIFSIQQTQSLPKP 
Sbjct: 121 PGDISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPS 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           K+SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA
Sbjct: 181 KFSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVG SSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPE+FQVSFNVTAEFEKLLRSKITNASPVI SSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of CmaCh16G003780 vs. NCBI nr
Match: XP_023552908.1 (12S seed storage globulin 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.9 bits (1740), Expect = 3.9e-190
Identity = 341/352 (96.88%), Postives = 344/352 (97.73%), Query Frame = 0

Query: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60
           MEQPMNPKPFTEVEAGSYHKWLPSEYPLLA NKVAAGRLLLRPRGFVVPHYADCSKVGYV
Sbjct: 1   MEQPMNPKPFTEVEAGSYHKWLPSEYPLLARNKVAAGRLLLRPRGFVVPHYADCSKVGYV 60

Query: 61  LQGENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120
           LQGENGV GLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV
Sbjct: 61  LQGENGVVGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHV 120

Query: 121 PGDISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPP 180
           PGDISYFVLSG LSLL+GFSPEYVG+TYSLNGEETTQFLKSQSNALI SIQQTQSLPKPP
Sbjct: 121 PGDISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALICSIQQTQSLPKPP 180

Query: 181 KYSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240
           K+SKFVYNIDAAAPDGRVKG AGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA
Sbjct: 181 KFSKFVYNIDAAAPDGRVKGSAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 240

Query: 241 EPYDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITA 300
           EPYDQLIYVAKGRGKIQIVG SSKIDAEVKMGQLILVPKFFAVGK AGEDGLECISIITA
Sbjct: 241 EPYDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKFAGEDGLECISIITA 300

Query: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVI SSD
Sbjct: 301 THPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIGSSD 352

BLAST of CmaCh16G003780 vs. NCBI nr
Match: KAG7014994.1 (12S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 547.7 bits (1410), Expect = 7.1e-152
Identity = 289/350 (82.57%), Postives = 294/350 (84.00%), Query Frame = 0

Query: 3   QPMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQ 62
           QPMNPKPFTEVEAGSYHKWLPSEYPLLA N                              
Sbjct: 320 QPMNPKPFTEVEAGSYHKWLPSEYPLLARN------------------------------ 379

Query: 63  GENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPG 122
                                KKGDLIPVPNGVSSWWFN+GDSDLEIIFLGESKNAHVPG
Sbjct: 380 ---------------------KKGDLIPVPNGVSSWWFNEGDSDLEIIFLGESKNAHVPG 439

Query: 123 DISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKY 182
           DISYFVLSG LSLL+GFSPEYVG+TYSLNGEETTQFLKSQSNALIFSIQQTQSLPKP K+
Sbjct: 440 DISYFVLSGPLSLLHGFSPEYVGKTYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPSKF 499

Query: 183 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 242
           SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP
Sbjct: 500 SKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEP 559

Query: 243 YDQLIYVAKGRGKIQIVGFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 302
           YDQLIYVAKGRGKIQIVG SSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH
Sbjct: 560 YDQLIYVAKGRGKIQIVGSSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATH 618

Query: 303 PVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNASPVIRSSD 353
           PVVEELAGKTSVLEALSPE+FQVSFNVTAEFEKLLRSKITNASPVI SSD
Sbjct: 620 PVVEELAGKTSVLEALSPEIFQVSFNVTAEFEKLLRSKITNASPVIGSSD 618

BLAST of CmaCh16G003780 vs. TAIR 10
Match: AT1G07750.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.1e-65
Identity = 134/346 (38.73%), Postives = 196/346 (56.65%), Query Frame = 0

Query: 4   PMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQG 63
           P  PK     + GSY  W P E P+L    + A +L L   GF VP Y+D SKV YVLQG
Sbjct: 7   PKLPKKVYGGDGGSYSAWCPEELPMLKQGNIGAAKLALEKNGFAVPRYSDSSKVAYVLQG 66

Query: 64  ENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPGD 123
            +G AG+V P K +E V+ +K+GD I +P GV +WWFN+ D +L I+FLGE+   H  G 
Sbjct: 67  -SGTAGIVLPEK-EEKVIAIKQGDSIALPFGVVTWWFNNEDPELVILFLGETHKGHKAGQ 126

Query: 124 ISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKYS 183
            + F L+G   +  GFS E+VG  + L+     + + SQ+   I  +     +P+P + +
Sbjct: 127 FTEFYLTGTNGIFTGFSTEFVGRAWDLDENTVKKLVGSQTGNGIVKLDAGFKMPQPKEEN 186

Query: 184 K--FVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 243
           +  FV N   A  D  +K G G V  +     P +G+ G  A L ++DA+++ SP +  +
Sbjct: 187 RAGFVLNCLEAPLDVDIKDG-GRVVVLNTKNLPLVGEVGFGADLVRIDAHSMCSPGFSCD 246

Query: 244 PYDQLIYVAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIIT 303
              Q+ Y+  G G++Q+VG   K  ++  +K G L +VP+FF V KIA  DG+   SI+T
Sbjct: 247 SALQVTYIVGGSGRVQVVGGDGKRVLETHIKAGSLFIVPRFFVVSKIADADGMSWFSIVT 306

Query: 304 ATHPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITNAS 346
              P+   LAG TSV ++LSPEV Q +F V  E EK  RS  T+++
Sbjct: 307 TPDPIFTHLAGNTSVWKSLSPEVLQAAFKVAPEVEKSFRSTRTSSA 349

BLAST of CmaCh16G003780 vs. TAIR 10
Match: AT2G28680.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 4.1e-65
Identity = 136/344 (39.53%), Postives = 193/344 (56.10%), Query Frame = 0

Query: 4   PMNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQG 63
           P  PK     + GSY  W P E P+L    + A +L L   G  +P Y+D  KV YVLQG
Sbjct: 7   PRLPKKVYGGDGGSYFAWCPEELPMLRDGNIGASKLALEKYGLALPRYSDSPKVAYVLQG 66

Query: 64  ENGVAGLVFPSKSDEVVVNLKKGDLIPVPNGVSSWWFNDGDSDLEIIFLGESKNAHVPGD 123
             G AG+V P K +E V+ +KKGD I +P GV +WWFN+ D++L ++FLGE+   H  G 
Sbjct: 67  A-GTAGIVLPEK-EEKVIAIKKGDSIALPFGVVTWWFNNEDTELVVLFLGETHKGHKAGQ 126

Query: 124 ISYFVLSGILSLLNGFSPEYVGETYSLNGEETTQFLKSQSNALIFSIQQTQSLPKPPKYS 183
            + F L+G   +  GFS E+VG  + L+     + + SQ+   I  +  +  +P+P K  
Sbjct: 127 FTDFYLTGSNGIFTGFSTEFVGRAWDLDETTVKKLVGSQTGNGIVKVDASLKMPEPKKGD 186

Query: 184 K--FVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAE 243
           +  FV N   A  D  +K G G V  +     P +G+ G  A L ++D +++ SP +  +
Sbjct: 187 RKGFVLNCLEAPLDVDIKDG-GRVVVLNTKNLPLVGEVGFGADLVRIDGHSMCSPGFSCD 246

Query: 244 PYDQLIYVAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISIIT 303
              Q+ Y+  G G++QIVG   K  ++  VK G L +VP+FF V KIA  DGL   SI+T
Sbjct: 247 SALQVTYIVGGSGRVQIVGADGKRVLETHVKAGVLFIVPRFFVVSKIADSDGLSWFSIVT 306

Query: 304 ATHPVVEELAGKTSVLEALSPEVFQVSFNVTAEFEKLLRSKITN 344
              P+   LAG+TSV +ALSPEV Q +F V  E EK  RSK T+
Sbjct: 307 TPDPIFTHLAGRTSVWKALSPEVLQAAFKVDPEVEKAFRSKRTS 347

BLAST of CmaCh16G003780 vs. TAIR 10
Match: AT1G03890.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 111.3 bits (277), Expect = 1.6e-24
Identity = 103/393 (26.21%), Postives = 160/393 (40.71%), Query Frame = 0

Query: 7   PKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGENG 66
           P   T+ EAG    W     P L    V   R+ L+P    +P +     + YV+QGE G
Sbjct: 46  PAQATKFEAGQMEVW-DHMSPELRCAGVTVARITLQPNSIFLPAFFSPPALAYVVQGE-G 105

Query: 67  VAGLV---FPSKSDEV----------------------VVNLKKGDLIPVPNGVSSWWFN 126
           V G +    P    EV                      + N ++GD+     GVS WW+N
Sbjct: 106 VMGTIASGCPETFAEVEGSSGRGGGGDPGRRFEDMHQKLENFRRGDVFASLAGVSQWWYN 165

Query: 127 DGDSDLEIIFLGESKNAHVPGD--ISYFVLSGIL--------------SLLNGFSPEYVG 186
            GDSD  I+ + +  N     D     F L+G                +  +GF P  + 
Sbjct: 166 RGDSDAVIVIVLDVTNRENQLDQVPRMFQLAGSRTQEEEQPLTWPSGNNAFSGFDPNIIA 225

Query: 187 ETYSLNGEETTQFLKSQSNA--LIFSIQQTQSLPKPPK----------------YSKFVY 246
           E + +N E   Q    + N   +I +      +  PP+                 +K   
Sbjct: 226 EAFKINIETAKQLQNQKDNRGNIIRANGPLHFVIPPPREWQQDGIANGIEETYCTAKIHE 285

Query: 247 NIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVAEPYDQLI 306
           NID           AG ++T+     P +    L A+   L +  +  P + A  +  ++
Sbjct: 286 NIDDPERSDHFSTRAGRISTLNSLNLPVLRLVRLNALRGYLYSGGMVLPQWTANAH-TVL 345

Query: 307 YVAKGRGKIQIV--GFSSKIDAEVKMGQLILVPKFFAVGKIAGEDGLECISIITATHPVV 339
           YV  G+ KIQ+V     S  + +V  GQ+I++P+ FAV K AGE G E IS  T  +  +
Sbjct: 346 YVTGGQAKIQVVDDNGQSVFNEQVGQGQIIVIPQGFAVSKTAGETGFEWISFKTNDNAYI 405

BLAST of CmaCh16G003780 vs. TAIR 10
Match: AT1G03880.1 (cruciferin 2 )

HSP 1 Score: 100.5 bits (249), Expect = 2.8e-21
Identity = 92/414 (22.22%), Postives = 171/414 (41.30%), Query Frame = 0

Query: 5   MNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGE 64
           + P    + E G    W     P L  +  A  R ++ P+G  +P + +  K+ +V+ G 
Sbjct: 38  LEPSQIIKSEGGRIEVW-DHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHG- 97

Query: 65  NGVAGLVFPSKSD------------------------EVVVNLKKGDLIPVPNGVSSWWF 124
            G+ G V P  ++                        + V +L+ GD I  P+GV+ W++
Sbjct: 98  RGLMGRVIPGCAETFMESPVFGEGQGQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQWFY 157

Query: 125 NDGDSDLEIIFLGE--SKNAHVPGDISYFVLSG----------------ILSLLNGFSPE 184
           N+G+  L ++   +  S    +  ++  F+++G                  ++ NGF+PE
Sbjct: 158 NNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPE 217

Query: 185 YVGETYSLNGEETTQFLKSQSN-ALIFSIQQTQSLPKPPK-------------------- 244
            + + + +N E   Q    Q N   I  +     + +PP                     
Sbjct: 218 ILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETL 277

Query: 245 -YSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANAVRSPVYVA 304
              +   N+D  +     K   G ++T+     P +    L+A+   +  NA+  P +  
Sbjct: 278 CTMRCTENLDDPSDADVYKPSLGYISTLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNV 337

Query: 305 EPYDQLIYVAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGEDGLECISII 348
              +  +YV  G+  IQ+V  + +   D E+  GQL++VP+ F+V K A  +  E I   
Sbjct: 338 NA-NAALYVTNGKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFK 397

BLAST of CmaCh16G003780 vs. TAIR 10
Match: AT5G44120.3 (RmlC-like cupins superfamily protein )

HSP 1 Score: 89.7 bits (221), Expect = 4.9e-18
Identity = 91/402 (22.64%), Postives = 163/402 (40.55%), Query Frame = 0

Query: 5   MNPKPFTEVEAGSYHKWLPSEYPLLAHNKVAAGRLLLRPRGFVVPHYADCSKVGYVLQGE 64
           + P    + EAG    W     P L  + V+  R ++  +G  +P + + +K+ +V +G 
Sbjct: 44  LEPSHVLKSEAGRIEVW-DHHAPQLRCSGVSFARYIIESKGLYLPSFFNTAKLSFVAKG- 103

Query: 65  NGVAGLVFPSKSD-------------------------EVVVNLKKGDLIPVPNGVSSWW 124
            G+ G V P  ++                         + V +++ GD I    GV+ W+
Sbjct: 104 RGLMGKVIPGCAETFQDSSEFQPRFEGQGQSQRFRDMHQKVEHIRSGDTIATTPGVAQWF 163

Query: 125 FNDGDSDLEIIFLGE--SKNAHVPGDISYFVLSG----------------ILSLLNGFSP 184
           +NDG   L I+ + +  S    +  +   F L+G                  ++ NGF P
Sbjct: 164 YNDGQEPLVIVSVFDLASHQNQLDRNPRPFYLAGNNPQGQVWLQGREQQPQKNIFNGFGP 223

Query: 185 EYVGETYSLNGEETTQFLKSQ--SNALIFSIQQTQSLPKPPK------------------ 244
           E + +   ++  +T Q L++Q  +   I  +Q    + +PP                   
Sbjct: 224 EVIAQALKID-LQTAQQLQNQDDNRGNIVRVQGPFGVIRPPLRGQRPQEEEEEEGRHGRH 283

Query: 245 ---------YSKFVYNIDAAAPDGRVKGGAGAVTTVTESKFPFIGQSGLTAILEKLDANA 304
                     ++   N+D  +     K   G ++T+     P +    L+A+   +  NA
Sbjct: 284 GNGLEETICSARCTDNLDDPSRADVYKPQLGYISTLNSYDLPILRFIRLSALRGSIRQNA 343

Query: 305 VRSPVYVAEPYDQLIYVAKGRGKIQIVGFSSK--IDAEVKMGQLILVPKFFAVGKIAGED 333
           +  P + A   + ++YV  G  +IQIV  +     D +V  GQLI VP+ F+V K A  +
Sbjct: 344 MVLPQWNANA-NAILYVTDGEAQIQIVNDNGNRVFDGQVSQGQLIAVPQGFSVVKRATSN 403

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZWA92.2e-2326.2112S seed storage protein CRD OS=Arabidopsis thaliana OX=3702 GN=CRD PE=1 SV=1[more]
P143234.7e-2123.26Glutelin type-B 1 OS=Oryza sativa subsp. japonica OX=39947 GN=GluB1-A PE=2 SV=3[more]
Q028974.7e-2123.04Glutelin type-B 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUB2 PE=2 SV=2[more]
P077301.8e-2021.94Glutelin type-A 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GLUA2 PE=1 SV=1[more]
P154563.9e-2022.2212S seed storage protein CRB OS=Arabidopsis thaliana OX=3702 GN=CRB PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1JDB25.4e-198100.0012S seed storage protein CRD-like OS=Cucurbita maxima OX=3661 GN=LOC111483370 PE... [more]
A0A6J1E9P27.6e-19297.44legumin J-like OS=Cucurbita moschata OX=3662 GN=LOC111430654 PE=4 SV=1[more]
A0A5A7T7U86.5e-15177.88Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold8... [more]
A0A1S3C2D56.5e-15177.88glutelin type-A 2-like OS=Cucumis melo OX=3656 GN=LOC103496119 PE=3 SV=1[more]
A0A5D3BLA41.2e-14977.98Glutelin type-A 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2... [more]
Match NameE-valueIdentityDescription
XP_022985328.11.1e-197100.0012S seed storage protein CRD-like [Cucurbita maxima][more]
XP_022922755.11.6e-19197.44legumin J-like [Cucurbita moschata][more]
KAG6576976.13.5e-19197.1612S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023552908.13.9e-19096.8812S seed storage globulin 1-like [Cucurbita pepo subsp. pepo][more]
KAG7014994.17.1e-15282.5712S seed storage protein CRD, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
AT1G07750.14.1e-6538.73RmlC-like cupins superfamily protein [more]
AT2G28680.14.1e-6539.53RmlC-like cupins superfamily protein [more]
AT1G03890.11.6e-2426.21RmlC-like cupins superfamily protein [more]
AT1G03880.12.8e-2122.22cruciferin 2 [more]
AT5G44120.34.9e-1822.64RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 187..335
e-value: 2.3E-14
score: 63.7
coord: 3..156
e-value: 1.2E-23
score: 94.6
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 6..154
e-value: 5.5E-20
score: 71.5
coord: 192..333
e-value: 9.4E-18
score: 64.2
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 6..176
e-value: 1.0E-29
score: 105.0
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 194..351
e-value: 6.8E-37
score: 128.3
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 3..343
NoneNo IPR availablePANTHERPTHR31189:SF4511S GLOBULIN SEED STORAGE PROTEIN 2-LIKEcoord: 3..343
NoneNo IPR availableCDDcd02242cupin_11S_legumin_Ncoord: 3..164
e-value: 2.26648E-54
score: 175.851
NoneNo IPR availableCDDcd02243cupin_11S_legumin_Ccoord: 201..348
e-value: 1.01939E-57
score: 182.288
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 3..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G003780.1CmaCh16G003780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0045735 nutrient reservoir activity