CmaCh16G002490 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G002490
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionMFS domain-containing protein
LocationCma_Chr16: 1156717 .. 1158045 (+)
RNA-Seq ExpressionCmaCh16G002490
SyntenyCmaCh16G002490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAATAATGAAAATTCCGAAGCGTTATTGGATTGTTCTTTTAACCTTCATCTGCACATCTGTTTGTTACGTGGAGCGTGTTGGCTTCTCGATTGCTTATACCTTTGCAGCTGATGCTGTGGGGGTAGACCAATCAAGCAAAGGCACTATTCTTTCAACATTTTACTATGGATATGCCTGTTCCCAGGTTCCTGGAGGATGGGCTGCTCAAAAAATTGGAGGTAGAAGGGTTCTTCTGATCTCATTTATTTTATGGTCCCTTACCTGTGCATTAGTTCCACTGGATCCGAGTAGGGTGATGGTCTTGGTAATTGCTCGGTTGCTTGTCGGTGTGGCTCAAGGTTTCATCTTTCCTTCAATCCACACTGTTTTAGCTCAGTGGGTTCCTCCTCATGAAAGATCAAGATCTGTATCTCTTACAACCTCTGGAATGTACCTAGGTGCCGCTGCAGGCATGCTTATCCTTCCAAGCCTGGTAAAGTATAGAGGGCCTCAGTCAGTATTTGCTGCTGAGGCATTGTTAGGTGCCACATGGTCTGTGCTTTGGCTCAAGTATGCAACCGACCCTCCACGTTCCGAACATCCCAAAGCTGCTGCTGCAGGTTTTGGTGAACCCCTGCTGCCAATCAAAGGAAACCAGAAAGTAAGAGTAGAGAGCGGAGGAACTTCAACACGAACCTCAAACATCCCGTGGAAGAAAATCTTGTTGAGCTTGCCAGTTTGGGCAATCGTGGTAAACAATTTTACCTTCCATTATGCTTTATATGTGTTGATGAACTGGCTGCCTACTTACTTTGAGCTTGGTCTTCAACTTAGCCTCCAGGAGATGGGTTCCTCTAAGATGTTGCCTTATCTTAACATGTTCTTATTCTCGAATGTCGGTGGGATAGCTGCTGATTACTTGGTCACTAAAAGGATTTTATCTGTTACTCGAACCCGGAAATTCTTGAATACGACAGGCTTTTCTGTAGCATCAATGGCACTGATGGCCATCCCCATCTTCAGAACTTCTAGTGGGGCTATCTTCTGCTCATCTGTGGCTCTTGGTTTCTTAGCTTTAGGGAGAGCAGGATTTGCTGTGAATCATATGGATATCGCTCCTCGATATGCTGGTATCGTCATGGGAGTCTCGAACACTGCTGGTACGCTAGCTGGTATCATCGGGGTTGATTTTACTGGGAAATTACTGGAAGCTGCGAAGGCTGCCAATTCAGATCTCTCAAGTCCAGAAAGCTGGAGAGCAGTTTTCTCCATACCGGGGCTGCTCTGCGTTCTTAGCTCTATTGTATTTTTGCTGTTTTCAACAGGTGAGAGAATTTTTGACTGA

mRNA sequence

ATGTCAATAATGAAAATTCCGAAGCGTTATTGGATTGTTCTTTTAACCTTCATCTGCACATCTGTTTGTTACGTGGAGCGTGTTGGCTTCTCGATTGCTTATACCTTTGCAGCTGATGCTGTGGGGGTAGACCAATCAAGCAAAGGCACTATTCTTTCAACATTTTACTATGGATATGCCTGTTCCCAGGTTCCTGGAGGATGGGCTGCTCAAAAAATTGGAGGTAGAAGGGTTCTTCTGATCTCATTTATTTTATGGTCCCTTACCTGTGCATTAGTTCCACTGGATCCGAGTAGGGTGATGGTCTTGGTAATTGCTCGGTTGCTTGTCGGTGTGGCTCAAGGTTTCATCTTTCCTTCAATCCACACTGTTTTAGCTCAGTGGGTTCCTCCTCATGAAAGATCAAGATCTGTATCTCTTACAACCTCTGGAATGTACCTAGGTGCCGCTGCAGGCATGCTTATCCTTCCAAGCCTGGTAAAGTATAGAGGGCCTCAGTCAGTATTTGCTGCTGAGGCATTGTTAGGTGCCACATGGTCTGTGCTTTGGCTCAAGTATGCAACCGACCCTCCACGTTCCGAACATCCCAAAGCTGCTGCTGCAGGTTTTGGTGAACCCCTGCTGCCAATCAAAGGAAACCAGAAAGTAAGAGTAGAGAGCGGAGGAACTTCAACACGAACCTCAAACATCCCGTGGAAGAAAATCTTGTTGAGCTTGCCAGTTTGGGCAATCGTGGTAAACAATTTTACCTTCCATTATGCTTTATATGTGTTGATGAACTGGCTGCCTACTTACTTTGAGCTTGGTCTTCAACTTAGCCTCCAGGAGATGGGTTCCTCTAAGATGTTGCCTTATCTTAACATGTTCTTATTCTCGAATGTCGGTGGGATAGCTGCTGATTACTTGGTCACTAAAAGGATTTTATCTGTTACTCGAACCCGGAAATTCTTGAATACGACAGGCTTTTCTGTAGCATCAATGGCACTGATGGCCATCCCCATCTTCAGAACTTCTAGTGGGGCTATCTTCTGCTCATCTGTGGCTCTTGGTTTCTTAGCTTTAGGGAGAGCAGGATTTGCTGTGAATCATATGGATATCGCTCCTCGATATGCTGGTATCGTCATGGGAGTCTCGAACACTGCTGGTACGCTAGCTGGTATCATCGGGGTTGATTTTACTGGGAAATTACTGGAAGCTGCGAAGGCTGCCAATTCAGATCTCTCAAGTCCAGAAAGCTGGAGAGCAGTTTTCTCCATACCGGGGCTGCTCTGCGTTCTTAGCTCTATTGTATTTTTGCTGTTTTCAACAGGTGAGAGAATTTTTGACTGA

Coding sequence (CDS)

ATGTCAATAATGAAAATTCCGAAGCGTTATTGGATTGTTCTTTTAACCTTCATCTGCACATCTGTTTGTTACGTGGAGCGTGTTGGCTTCTCGATTGCTTATACCTTTGCAGCTGATGCTGTGGGGGTAGACCAATCAAGCAAAGGCACTATTCTTTCAACATTTTACTATGGATATGCCTGTTCCCAGGTTCCTGGAGGATGGGCTGCTCAAAAAATTGGAGGTAGAAGGGTTCTTCTGATCTCATTTATTTTATGGTCCCTTACCTGTGCATTAGTTCCACTGGATCCGAGTAGGGTGATGGTCTTGGTAATTGCTCGGTTGCTTGTCGGTGTGGCTCAAGGTTTCATCTTTCCTTCAATCCACACTGTTTTAGCTCAGTGGGTTCCTCCTCATGAAAGATCAAGATCTGTATCTCTTACAACCTCTGGAATGTACCTAGGTGCCGCTGCAGGCATGCTTATCCTTCCAAGCCTGGTAAAGTATAGAGGGCCTCAGTCAGTATTTGCTGCTGAGGCATTGTTAGGTGCCACATGGTCTGTGCTTTGGCTCAAGTATGCAACCGACCCTCCACGTTCCGAACATCCCAAAGCTGCTGCTGCAGGTTTTGGTGAACCCCTGCTGCCAATCAAAGGAAACCAGAAAGTAAGAGTAGAGAGCGGAGGAACTTCAACACGAACCTCAAACATCCCGTGGAAGAAAATCTTGTTGAGCTTGCCAGTTTGGGCAATCGTGGTAAACAATTTTACCTTCCATTATGCTTTATATGTGTTGATGAACTGGCTGCCTACTTACTTTGAGCTTGGTCTTCAACTTAGCCTCCAGGAGATGGGTTCCTCTAAGATGTTGCCTTATCTTAACATGTTCTTATTCTCGAATGTCGGTGGGATAGCTGCTGATTACTTGGTCACTAAAAGGATTTTATCTGTTACTCGAACCCGGAAATTCTTGAATACGACAGGCTTTTCTGTAGCATCAATGGCACTGATGGCCATCCCCATCTTCAGAACTTCTAGTGGGGCTATCTTCTGCTCATCTGTGGCTCTTGGTTTCTTAGCTTTAGGGAGAGCAGGATTTGCTGTGAATCATATGGATATCGCTCCTCGATATGCTGGTATCGTCATGGGAGTCTCGAACACTGCTGGTACGCTAGCTGGTATCATCGGGGTTGATTTTACTGGGAAATTACTGGAAGCTGCGAAGGCTGCCAATTCAGATCTCTCAAGTCCAGAAAGCTGGAGAGCAGTTTTCTCCATACCGGGGCTGCTCTGCGTTCTTAGCTCTATTGTATTTTTGCTGTTTTCAACAGGTGAGAGAATTTTTGACTGA

Protein sequence

MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGLLCVLSSIVFLLFSTGERIFD
Homology
BLAST of CmaCh16G002490 vs. ExPASy Swiss-Prot
Match: Q9FKV1 (Probable anion transporter 5 OS=Arabidopsis thaliana OX=3702 GN=ANTR5 PE=2 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.1e-196
Identity = 347/442 (78.51%), Postives = 383/442 (86.65%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           M +  IP+RY IV LTF+ T VCY+ERVGFSIAYT AADA G++QSSKGTILSTF+ GYA
Sbjct: 1   MKLSNIPQRYVIVFLTFLSTCVCYIERVGFSIAYTVAADAAGINQSSKGTILSTFFVGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGR+VLL+SF+LWS TC LVPLDP+RV +LV+ARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRKVLLLSFVLWSSTCFLVPLDPNRVGLLVVARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSR VS+TTSGMYLGAA GM +LP+LV+ RGP+SVF AEAL G  WS
Sbjct: 121 IHTVLAQWVPPHERSRLVSITTSGMYLGAALGMWLLPALVELRGPESVFLAEALAGVIWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           +LW++YATDPPRSEHPKAAAAGFG  LLP   N            + ++IPWKKI+LSLP
Sbjct: 181 LLWIRYATDPPRSEHPKAAAAGFGGALLPTNVNHH----------KVTHIPWKKIMLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQ+SLQ M SSKM+PYLNMF+FS VGG  AD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQISLQGMDSSKMVPYLNMFVFSIVGGFIAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YL+TKRILSVTRTRKFLNT GF +AS ALM +P+FRT +G I CSSVALGFLALGRAGFA
Sbjct: 301 YLITKRILSVTRTRKFLNTVGFLIASAALMVLPMFRTENGVILCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEA+K   SDLS PESWR VF IP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEASKLVYSDLSHPESWRVVFFIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+ SS+VFLLFSTGERIFD
Sbjct: 421 GLLCIFSSVVFLLFSTGERIFD 432

BLAST of CmaCh16G002490 vs. ExPASy Swiss-Prot
Match: Q53P54 (Probable anion transporter 6 OS=Oryza sativa subsp. japonica OX=39947 GN=PHT4;6 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 6.4e-185
Identity = 317/440 (72.05%), Postives = 381/440 (86.59%), Query Frame = 0

Query: 4   MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQ 63
           MK PKRY IVLLTF+CT+VCY+ERVGFSIAYT AADAVG +Q++KG ILS FYYGY  SQ
Sbjct: 1   MKFPKRYAIVLLTFMCTNVCYIERVGFSIAYTVAADAVGTNQANKGMILSMFYYGYVLSQ 60

Query: 64  VPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHT 123
           +PGGWAAQ++GGR VLL+SF+LWS  CA+VPLDP+RV++LV++RLLVGVAQG IFPSIHT
Sbjct: 61  IPGGWAAQRLGGRLVLLLSFVLWSSICAVVPLDPNRVILLVLSRLLVGVAQGLIFPSIHT 120

Query: 124 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 183
           VLAQWVPP ERSRSVSLTTSGMYLGAA GML+LPSLVK  GPQSVF+ EA+LG  W ++W
Sbjct: 121 VLAQWVPPQERSRSVSLTTSGMYLGAACGMLLLPSLVKNMGPQSVFSVEAMLGVAWLLIW 180

Query: 184 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGG-TSTRTSNIPWKKILLSLPVW 243
            K+A+DPPR++ PK A+              K++V++GG  + RT  IPW +IL SLP+W
Sbjct: 181 FKFASDPPRTDLPKVAS------------KDKMKVQTGGIMAPRTVKIPWARILFSLPIW 240

Query: 244 AIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYL 303
           AIVVNNFTFHYALYVLMNWLPTYF+LGLQLSLQ+MG SKMLPYLNMFLFSN+GG+ AD+L
Sbjct: 241 AIVVNNFTFHYALYVLMNWLPTYFKLGLQLSLQDMGFSKMLPYLNMFLFSNIGGVLADHL 300

Query: 304 VTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVN 363
           +T++ILSVT+TRK LNT GF V+++ALMA+P+FRT SGAIFCSSV+LGFLALGRAGFAVN
Sbjct: 301 ITRKILSVTKTRKLLNTVGFVVSAIALMALPLFRTPSGAIFCSSVSLGFLALGRAGFAVN 360

Query: 364 HMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGL 423
           HMD+AP++AGIVMG+SNTAGTLAGI+GV  TG++LEAAKA+N DL+S ESWR VF +PG 
Sbjct: 361 HMDVAPKFAGIVMGISNTAGTLAGIVGVGLTGRILEAAKASNMDLTSSESWRTVFFVPGY 420

Query: 424 LCVLSSIVFLLFSTGERIFD 443
           LC+ SS +FL+FSTGE+IF+
Sbjct: 421 LCIFSSFIFLIFSTGEKIFE 428

BLAST of CmaCh16G002490 vs. ExPASy Swiss-Prot
Match: Q2QWW7 (Probable anion transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=PHT4;7 PE=2 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 3.2e-184
Identity = 309/440 (70.23%), Postives = 379/440 (86.14%), Query Frame = 0

Query: 4   MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQ 63
           MK PKRY IVLLTFICT+VCY+ERVGFSIAYT AADA+GV+Q++KG ILS FYYGY  SQ
Sbjct: 7   MKFPKRYVIVLLTFICTNVCYIERVGFSIAYTVAADAIGVNQANKGMILSMFYYGYVLSQ 66

Query: 64  VPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHT 123
           +PGGWAAQ+IGGRRVLL+SF+LWSL C L+PLDP R ++LV++RL VGVAQGFIFP+IHT
Sbjct: 67  IPGGWAAQRIGGRRVLLLSFVLWSLICGLIPLDPKREVILVLSRLFVGVAQGFIFPAIHT 126

Query: 124 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 183
           VLAQWVPP ERSRSVSLTTSGMYLGAA GML  PSLVK+ G QSVF  EA+LG  WSV+W
Sbjct: 127 VLAQWVPPQERSRSVSLTTSGMYLGAAGGMLFFPSLVKHMGAQSVFFVEAVLGVAWSVIW 186

Query: 184 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGG-TSTRTSNIPWKKILLSLPVW 243
           LK++++PPR++ PK +       +  +   +K++ ++GG  + RT  IPW++I+ SLPVW
Sbjct: 187 LKFSSEPPRTDLPKVS-------MPKVASREKIKAQAGGVVAPRTVKIPWRRIIFSLPVW 246

Query: 244 AIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYL 303
           AIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQ+MGSSKMLPY NMF+FSN+GG+ AD+L
Sbjct: 247 AIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQDMGSSKMLPYFNMFIFSNIGGVVADHL 306

Query: 304 VTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVN 363
           +T+RILS+T+TRK LNT GF V+++ALMA+P+FRT SG + CSS++LGFLALGRAGFAVN
Sbjct: 307 ITRRILSITKTRKLLNTIGFVVSAVALMALPLFRTPSGTVLCSSISLGFLALGRAGFAVN 366

Query: 364 HMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGL 423
           HMD+AP++AGIVMGVSNTAGTLAGI+GV  TG +LE AKA+N DL++ E+W+ VF +PG 
Sbjct: 367 HMDVAPKFAGIVMGVSNTAGTLAGIVGVGLTGSILEGAKASNMDLTNSETWKTVFFVPGY 426

Query: 424 LCVLSSIVFLLFSTGERIFD 443
           LC+ SSI+FL+FSTGE+IF+
Sbjct: 427 LCIFSSIIFLIFSTGEKIFE 439

BLAST of CmaCh16G002490 vs. ExPASy Swiss-Prot
Match: Q652N5 (Probable anion transporter 4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=PHT4;4 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 8.0e-71
Identity = 156/441 (35.37%), Postives = 252/441 (57.14%), Query Frame = 0

Query: 5   KIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQV 64
           + PKR+ +VLL F    +C ++RV  SIA    +   G   ++ G I S+F++GY  +Q+
Sbjct: 177 QFPKRWTVVLLCFFSFLLCNMDRVNMSIAILPMSSEFGWSPATVGLIQSSFFWGYLLTQI 236

Query: 65  PGGWAAQKIGGRRVLLISFILWSLTCALVPLDPS-RVMVLVIARLLVGVAQGFIFPSIHT 124
            GG  A + GG+ VL    + WS+   L PL     +  L++ R  +G+ +G   P+++ 
Sbjct: 237 LGGIWADRFGGKVVLGFGVVWWSIATVLTPLAAKIGLPFLLVMRAFMGIGEGVAMPAMNN 296

Query: 125 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 184
           +L++WVP  ERSRS++L  SGMYLG+  G+   P L+   G  SVF A   LG+ W  LW
Sbjct: 297 ILSKWVPVSERSRSLALVYSGMYLGSVTGLAFSPLLISRFGWPSVFYAFGSLGSVWFALW 356

Query: 185 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTR--TSNIPWKKILLSLPV 244
            + A   P SE P+ + A             + R   GG++ +   ++IPWK IL   PV
Sbjct: 357 QRKAHSSP-SEDPELSKA-------------EKRYILGGSTLKEPVTSIPWKLILSKPPV 416

Query: 245 WAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADY 304
           WA++V++F  ++  ++L+ W+PTY+   L+ +L E G   +LP+L M +F+N+GG  AD 
Sbjct: 417 WALIVSHFCHNWGTFILLTWMPTYYNQVLKFNLTESGLLCVLPWLTMAIFANIGGWIADT 476

Query: 305 LVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAV 364
           LV + + S+T  RK + + GF   ++ L  +   RT + A+ C + + G  A  ++G   
Sbjct: 477 LVGRGV-SITNVRKIMQSIGFLGPALFLTLLSKVRTPAMAVLCMACSQGSDAFSQSGLYS 536

Query: 365 NHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPG 424
           NH DI PRYAG+++G+SNTAG LAG+ G   TG +L+             SW +VF +  
Sbjct: 537 NHQDIGPRYAGVLLGLSNTAGVLAGVFGTAATGYILQKG-----------SWDSVFQVAV 591

Query: 425 LLCVLSSIVFLLFSTGERIFD 443
           +L ++ ++V+ +FSTGE++ +
Sbjct: 597 VLYIVGTVVWNVFSTGEKVLE 591

BLAST of CmaCh16G002490 vs. ExPASy Swiss-Prot
Match: Q8GX78 (Ascorbate transporter, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHT4;4 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.2e-69
Identity = 154/441 (34.92%), Postives = 248/441 (56.24%), Query Frame = 0

Query: 5   KIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQV 64
           + P+R+ IVLL F    +C ++RV  SIA    +       ++ G I S+F++GY  +Q+
Sbjct: 127 QFPRRWVIVLLCFSSFLLCNMDRVNMSIAILPMSQEYNWSSATVGLIQSSFFWGYLLTQI 186

Query: 65  PGGWAAQKIGGRRVLLISFILWSLTCALVPLDPS-RVMVLVIARLLVGVAQGFIFPSIHT 124
            GG  A K GG+ VL    + WS    + P+     +  L++ R  +G+ +G   P+++ 
Sbjct: 187 LGGIWADKFGGKVVLGFGVVWWSFATIMTPIAARLGLPFLLVVRAFMGIGEGVAMPAMNN 246

Query: 125 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 184
           +L++W+P  ERSRS++L  SGMYLG+  G+   P L+   G  SVF +   LG+ W +LW
Sbjct: 247 MLSKWIPVSERSRSLALVYSGMYLGSVTGLAFSPMLITKFGWPSVFYSFGSLGSIWFLLW 306

Query: 185 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTR--TSNIPWKKILLSLPV 244
           LK+A   P+ +              P    ++ +V  GG+  R   + IPWK IL   PV
Sbjct: 307 LKFAYSSPKDD--------------PDLSEEEKKVILGGSKPREPVTVIPWKLILSKPPV 366

Query: 245 WAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADY 304
           WA+++++F  ++  ++L+ W+PTY+   L+ +L E G   +LP+L M +F+N+GG  AD 
Sbjct: 367 WALIISHFCHNWGTFILLTWMPTYYNQVLKFNLTESGLLCVLPWLTMAVFANIGGWIADT 426

Query: 305 LVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAV 364
           LV+ R LS+T  RK + + GF   +  L  +   +T + A+ C + + G  A  ++G   
Sbjct: 427 LVS-RGLSITNVRKIMQSIGFLGPAFFLSQLSHVKTPAMAVLCMACSQGSDAFSQSGLYS 486

Query: 365 NHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPG 424
           NH DI PRYAG+++G+SNTAG LAG+ G   TG +L+             SW  VF +  
Sbjct: 487 NHQDIGPRYAGVLLGLSNTAGVLAGVFGTAATGYILQRG-----------SWDDVFKVAV 541

Query: 425 LLCVLSSIVFLLFSTGERIFD 443
            L ++ ++V+ LF+TGE+I D
Sbjct: 547 ALYLIGTLVWNLFATGEKILD 541

BLAST of CmaCh16G002490 vs. ExPASy TrEMBL
Match: A0A6J1J9U7 (probable anion transporter 5 OS=Cucurbita maxima OX=3661 GN=LOC111482603 PE=4 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 3.9e-246
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA
Sbjct: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLCVLSSIVFLLFSTGERIFD
Sbjct: 421 GLLCVLSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. ExPASy TrEMBL
Match: A0A6J1E3G4 (probable anion transporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111430488 PE=4 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 8.2e-244
Identity = 437/442 (98.87%), Postives = 439/442 (99.32%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YLVTKRILSVTRTRKFLNT GF+VASMALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 YLVTKRILSVTRTRKFLNTIGFAVASMALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGI+GVD TGKLLEAAKAANSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKAANSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLCVLSSIVFLLFSTGERIFD
Sbjct: 421 GLLCVLSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. ExPASy TrEMBL
Match: A0A1S4E1C9 (probable anion transporter 5 OS=Cucumis melo OX=3656 GN=LOC103495770 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 425/442 (96.15%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MS+MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADA GVDQSSKGTILSTFYYGYA
Sbjct: 1   MSMMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAAGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGG+RVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGKRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFA EALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFATEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYA+DPPRSEHPKAAAAGFGE LLPIKGNQKV+VE+GGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYASDPPRSEHPKAAAAGFGESLLPIKGNQKVKVENGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSN+GGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNIGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           +LVTKRILSVTRTRKFLNT GF+VAS+ALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 HLVTKRILSVTRTRKFLNTIGFAVASLALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEAAKA NSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEAAKATNSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+LSSIVFLLFSTGERIFD
Sbjct: 421 GLLCILSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. ExPASy TrEMBL
Match: A0A5D3CTQ2 (Putative anion transporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1706G00250 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.4e-238
Identity = 425/442 (96.15%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MS+MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADA GVDQSSKGTILSTFYYGYA
Sbjct: 1   MSMMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAAGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGG+RVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGKRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFA EALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFATEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYA+DPPRSEHPKAAAAGFGE LLPIKGNQKV+VE+GGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYASDPPRSEHPKAAAAGFGESLLPIKGNQKVKVENGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSN+GGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNIGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           +LVTKRILSVTRTRKFLNT GF+VAS+ALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 HLVTKRILSVTRTRKFLNTIGFAVASLALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEAAKA NSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEAAKATNSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+LSSIVFLLFSTGERIFD
Sbjct: 421 GLLCILSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. ExPASy TrEMBL
Match: A0A0A0KVQ1 (MFS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G107370 PE=4 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.3e-238
Identity = 425/442 (96.15%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           M +MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MLMMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGG+RVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGKRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFA EALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFATEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYA+DPPRSEHPKAAAAGFGE LLPIKGNQKV+VE+GGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYASDPPRSEHPKAAAAGFGESLLPIKGNQKVKVENGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSN+GGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNIGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           +LVTKRILSVTRTRKFLNT GF+VAS+ALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 HLVTKRILSVTRTRKFLNTIGFAVASLALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEAAKA NSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEAAKATNSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+LSSIVFLLFSTGERIFD
Sbjct: 421 GLLCILSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. NCBI nr
Match: XP_022984219.1 (probable anion transporter 5 [Cucurbita maxima] >XP_022984220.1 probable anion transporter 5 [Cucurbita maxima] >XP_022984221.1 probable anion transporter 5 [Cucurbita maxima])

HSP 1 Score: 860.1 bits (2221), Expect = 8.1e-246
Identity = 442/442 (100.00%), Postives = 442/442 (100.00%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA
Sbjct: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLCVLSSIVFLLFSTGERIFD
Sbjct: 421 GLLCVLSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. NCBI nr
Match: XP_022922505.1 (probable anion transporter 5 [Cucurbita moschata] >XP_022922506.1 probable anion transporter 5 [Cucurbita moschata] >XP_022922507.1 probable anion transporter 5 [Cucurbita moschata] >XP_023552231.1 probable anion transporter 5 [Cucurbita pepo subsp. pepo] >XP_023552232.1 probable anion transporter 5 [Cucurbita pepo subsp. pepo] >XP_023552233.1 probable anion transporter 5 [Cucurbita pepo subsp. pepo] >XP_023552234.1 probable anion transporter 5 [Cucurbita pepo subsp. pepo] >KAG6576865.1 putative anion transporter 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.4 bits (2201), Expect = 1.7e-243
Identity = 437/442 (98.87%), Postives = 439/442 (99.32%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YLVTKRILSVTRTRKFLNT GF+VASMALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 YLVTKRILSVTRTRKFLNTIGFAVASMALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGI+GVD TGKLLEAAKAANSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKAANSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLCVLSSIVFLLFSTGERIFD
Sbjct: 421 GLLCVLSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. NCBI nr
Match: KAG7014888.1 (putative anion transporter 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 847.8 bits (2189), Expect = 4.2e-242
Identity = 434/439 (98.86%), Postives = 436/439 (99.32%), Query Frame = 0

Query: 4   MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQ 63
           MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQ
Sbjct: 1   MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQ 60

Query: 64  VPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHT 123
           VPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHT
Sbjct: 61  VPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPSIHT 120

Query: 124 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 183
           VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW
Sbjct: 121 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 180

Query: 184 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWA 243
           LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWA
Sbjct: 181 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWA 240

Query: 244 IVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLV 303
           IVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLV
Sbjct: 241 IVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLV 300

Query: 304 TKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVNH 363
           TKRILSVTRTRKFLNT GF+VASMALMAIPIFRTS GAIFCSSVALGFLALGRAGFAVNH
Sbjct: 301 TKRILSVTRTRKFLNTIGFAVASMALMAIPIFRTSGGAIFCSSVALGFLALGRAGFAVNH 360

Query: 364 MDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGLL 423
           MDIAPRYAGIVMGVSNTAGTLAGI+GVD TGKLLEAAKAANSDLSSPESWRAVFSIPGLL
Sbjct: 361 MDIAPRYAGIVMGVSNTAGTLAGIVGVDLTGKLLEAAKAANSDLSSPESWRAVFSIPGLL 420

Query: 424 CVLSSIVFLLFSTGERIFD 443
           CVLSSIVFLLFSTGERIFD
Sbjct: 421 CVLSSIVFLLFSTGERIFD 439

BLAST of CmaCh16G002490 vs. NCBI nr
Match: XP_008455652.1 (PREDICTED: probable anion transporter 5 [Cucumis melo] >XP_008455654.1 PREDICTED: probable anion transporter 5 [Cucumis melo] >XP_016901795.1 PREDICTED: probable anion transporter 5 [Cucumis melo] >KAA0066581.1 putative anion transporter 5 [Cucumis melo var. makuwa] >TYK15317.1 putative anion transporter 5 [Cucumis melo var. makuwa])

HSP 1 Score: 835.1 bits (2156), Expect = 2.8e-238
Identity = 425/442 (96.15%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           MS+MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADA GVDQSSKGTILSTFYYGYA
Sbjct: 1   MSMMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAAGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGG+RVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGKRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFA EALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFATEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYA+DPPRSEHPKAAAAGFGE LLPIKGNQKV+VE+GGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYASDPPRSEHPKAAAAGFGESLLPIKGNQKVKVENGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSN+GGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNIGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           +LVTKRILSVTRTRKFLNT GF+VAS+ALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 HLVTKRILSVTRTRKFLNTIGFAVASLALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEAAKA NSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEAAKATNSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+LSSIVFLLFSTGERIFD
Sbjct: 421 GLLCILSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. NCBI nr
Match: XP_004137171.1 (probable anion transporter 5 [Cucumis sativus] >XP_031740473.1 probable anion transporter 5 [Cucumis sativus])

HSP 1 Score: 834.3 bits (2154), Expect = 4.8e-238
Identity = 425/442 (96.15%), Postives = 435/442 (98.42%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           M +MKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA
Sbjct: 1   MLMMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGG+RVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGKRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFA EALLGATWS
Sbjct: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFATEALLGATWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           VLWLKYA+DPPRSEHPKAAAAGFGE LLPIKGNQKV+VE+GGTSTRTSNIPWKKILLSLP
Sbjct: 181 VLWLKYASDPPRSEHPKAAAAGFGESLLPIKGNQKVKVENGGTSTRTSNIPWKKILLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSN+GGIAAD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNIGGIAAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           +LVTKRILSVTRTRKFLNT GF+VAS+ALMAIPIFRTS GAIFCSSVALGFLALGRAGFA
Sbjct: 301 HLVTKRILSVTRTRKFLNTIGFAVASLALMAIPIFRTSGGAIFCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEAAKA NSDLSSPESWRAVFSIP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEAAKATNSDLSSPESWRAVFSIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+LSSIVFLLFSTGERIFD
Sbjct: 421 GLLCILSSIVFLLFSTGERIFD 442

BLAST of CmaCh16G002490 vs. TAIR 10
Match: AT5G44370.1 (phosphate transporter 4;6 )

HSP 1 Score: 686.4 bits (1770), Expect = 1.5e-197
Identity = 347/442 (78.51%), Postives = 383/442 (86.65%), Query Frame = 0

Query: 1   MSIMKIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYA 60
           M +  IP+RY IV LTF+ T VCY+ERVGFSIAYT AADA G++QSSKGTILSTF+ GYA
Sbjct: 1   MKLSNIPQRYVIVFLTFLSTCVCYIERVGFSIAYTVAADAAGINQSSKGTILSTFFVGYA 60

Query: 61  CSQVPGGWAAQKIGGRRVLLISFILWSLTCALVPLDPSRVMVLVIARLLVGVAQGFIFPS 120
           CSQVPGGWAAQKIGGR+VLL+SF+LWS TC LVPLDP+RV +LV+ARLLVGVAQGFIFPS
Sbjct: 61  CSQVPGGWAAQKIGGRKVLLLSFVLWSSTCFLVPLDPNRVGLLVVARLLVGVAQGFIFPS 120

Query: 121 IHTVLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWS 180
           IHTVLAQWVPPHERSR VS+TTSGMYLGAA GM +LP+LV+ RGP+SVF AEAL G  WS
Sbjct: 121 IHTVLAQWVPPHERSRLVSITTSGMYLGAALGMWLLPALVELRGPESVFLAEALAGVIWS 180

Query: 181 VLWLKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLP 240
           +LW++YATDPPRSEHPKAAAAGFG  LLP   N            + ++IPWKKI+LSLP
Sbjct: 181 LLWIRYATDPPRSEHPKAAAAGFGGALLPTNVNHH----------KVTHIPWKKIMLSLP 240

Query: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAAD 300
           VWAIVVNNFTFHYALYVLMNWLPTYFELGLQ+SLQ M SSKM+PYLNMF+FS VGG  AD
Sbjct: 241 VWAIVVNNFTFHYALYVLMNWLPTYFELGLQISLQGMDSSKMVPYLNMFVFSIVGGFIAD 300

Query: 301 YLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFA 360
           YL+TKRILSVTRTRKFLNT GF +AS ALM +P+FRT +G I CSSVALGFLALGRAGFA
Sbjct: 301 YLITKRILSVTRTRKFLNTVGFLIASAALMVLPMFRTENGVILCSSVALGFLALGRAGFA 360

Query: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIP 420
           VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVD TGKLLEA+K   SDLS PESWR VF IP
Sbjct: 361 VNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDLTGKLLEASKLVYSDLSHPESWRVVFFIP 420

Query: 421 GLLCVLSSIVFLLFSTGERIFD 443
           GLLC+ SS+VFLLFSTGERIFD
Sbjct: 421 GLLCIFSSVVFLLFSTGERIFD 432

BLAST of CmaCh16G002490 vs. TAIR 10
Match: AT4G00370.1 (Major facilitator superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 8.2e-71
Identity = 154/441 (34.92%), Postives = 248/441 (56.24%), Query Frame = 0

Query: 5   KIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQV 64
           + P+R+ IVLL F    +C ++RV  SIA    +       ++ G I S+F++GY  +Q+
Sbjct: 127 QFPRRWVIVLLCFSSFLLCNMDRVNMSIAILPMSQEYNWSSATVGLIQSSFFWGYLLTQI 186

Query: 65  PGGWAAQKIGGRRVLLISFILWSLTCALVPLDPS-RVMVLVIARLLVGVAQGFIFPSIHT 124
            GG  A K GG+ VL    + WS    + P+     +  L++ R  +G+ +G   P+++ 
Sbjct: 187 LGGIWADKFGGKVVLGFGVVWWSFATIMTPIAARLGLPFLLVVRAFMGIGEGVAMPAMNN 246

Query: 125 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 184
           +L++W+P  ERSRS++L  SGMYLG+  G+   P L+   G  SVF +   LG+ W +LW
Sbjct: 247 MLSKWIPVSERSRSLALVYSGMYLGSVTGLAFSPMLITKFGWPSVFYSFGSLGSIWFLLW 306

Query: 185 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTR--TSNIPWKKILLSLPV 244
           LK+A   P+ +              P    ++ +V  GG+  R   + IPWK IL   PV
Sbjct: 307 LKFAYSSPKDD--------------PDLSEEEKKVILGGSKPREPVTVIPWKLILSKPPV 366

Query: 245 WAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADY 304
           WA+++++F  ++  ++L+ W+PTY+   L+ +L E G   +LP+L M +F+N+GG  AD 
Sbjct: 367 WALIISHFCHNWGTFILLTWMPTYYNQVLKFNLTESGLLCVLPWLTMAVFANIGGWIADT 426

Query: 305 LVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAV 364
           LV+ R LS+T  RK + + GF   +  L  +   +T + A+ C + + G  A  ++G   
Sbjct: 427 LVS-RGLSITNVRKIMQSIGFLGPAFFLSQLSHVKTPAMAVLCMACSQGSDAFSQSGLYS 486

Query: 365 NHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPG 424
           NH DI PRYAG+++G+SNTAG LAG+ G   TG +L+             SW  VF +  
Sbjct: 487 NHQDIGPRYAGVLLGLSNTAGVLAGVFGTAATGYILQRG-----------SWDDVFKVAV 541

Query: 425 LLCVLSSIVFLLFSTGERIFD 443
            L ++ ++V+ LF+TGE+I D
Sbjct: 547 ALYLIGTLVWNLFATGEKILD 541

BLAST of CmaCh16G002490 vs. TAIR 10
Match: AT2G29650.1 (phosphate transporter 4;1 )

HSP 1 Score: 258.8 bits (660), Expect = 7.7e-69
Identity = 155/439 (35.31%), Postives = 243/439 (55.35%), Query Frame = 0

Query: 5   KIPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQV 64
           + PKR+ IVLL F    +C ++RV  SIA    +   G + ++ G I S+F++GY  +Q+
Sbjct: 98  EFPKRWVIVLLCFSAFLLCNMDRVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQI 157

Query: 65  PGGWAAQKIGGRRVLLISFILWSLTCALVPLDPS-RVMVLVIARLLVGVAQGFIFPSIHT 124
            GG  A  +GG+RVL    I WS+   L P+     +  L++ R  +GV +G   P+++ 
Sbjct: 158 AGGIWADTVGGKRVLGFGVIWWSIATILTPVAAKLGLPYLLVVRAFMGVGEGVAMPAMNN 217

Query: 125 VLAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLW 184
           +L++WVP  ERSRS++L  SGMYLG+  G+   P L+   G  SVF +   LG  W  LW
Sbjct: 218 ILSKWVPVQERSRSLALVYSGMYLGSVTGLAFSPFLIHQFGWPSVFYSFGSLGTVWLTLW 277

Query: 185 LKYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWA 244
           L  A   P  +            LLP    +K+  ++  +     +IPW+ IL   PVWA
Sbjct: 278 LTKAESSPLED----------PTLLP--EERKLIADNCASKEPVKSIPWRLILSKPPVWA 337

Query: 245 IVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLV 304
           ++  +F  ++  ++L+ W+PTY+   L+ +L E G   + P++ M + +N GG  AD LV
Sbjct: 338 LISCHFCHNWGTFILLTWMPTYYHQVLKFNLMESGLLSVFPWMTMAISANAGGWIADTLV 397

Query: 305 TKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVNH 364
           + R  SVT  RK + T GF   +  L  +    + + A+ C + + G  A  ++G   NH
Sbjct: 398 S-RGFSVTNVRKIMQTIGFLGPAFFLTQLKHIDSPTMAVLCMACSQGTDAFSQSGLYSNH 457

Query: 365 MDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGLL 424
            DIAPRY+G+++G+SNTAG LAG++G   TG +L+             SW  VF+I   L
Sbjct: 458 QDIAPRYSGVLLGLSNTAGVLAGVLGTAATGHILQHG-----------SWDDVFTISVGL 512

Query: 425 CVLSSIVFLLFSTGERIFD 443
            ++ ++++ LFSTGE+I D
Sbjct: 518 YLVGTVIWNLFSTGEKIID 512

BLAST of CmaCh16G002490 vs. TAIR 10
Match: AT2G29650.3 (phosphate transporter 4;1 )

HSP 1 Score: 244.2 bits (622), Expect = 2.0e-64
Identity = 146/418 (34.93%), Postives = 229/418 (54.78%), Query Frame = 0

Query: 26  ERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQVPGGWAAQKIGGRRVLLISFIL 85
           E V  SIA    +   G + ++ G I S+F++GY  +Q+ GG  A  +GG+RVL    I 
Sbjct: 7   EAVNMSIAILPMSAEYGWNPATVGLIQSSFFWGYLLTQIAGGIWADTVGGKRVLGFGVIW 66

Query: 86  WSLTCALVPLDPS-RVMVLVIARLLVGVAQGFIFPSIHTVLAQWVPPHERSRSVSLTTSG 145
           WS+   L P+     +  L++ R  +GV +G   P+++ +L++WVP  ERSRS++L  SG
Sbjct: 67  WSIATILTPVAAKLGLPYLLVVRAFMGVGEGVAMPAMNNILSKWVPVQERSRSLALVYSG 126

Query: 146 MYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLWLKYATDPPRSEHPKAAAAGFG 205
           MYLG+  G+   P L+   G  SVF +   LG  W  LWL  A   P  +          
Sbjct: 127 MYLGSVTGLAFSPFLIHQFGWPSVFYSFGSLGTVWLTLWLTKAESSPLED---------- 186

Query: 206 EPLLPIKGNQKVRVESGGTSTRTSNIPWKKILLSLPVWAIVVNNFTFHYALYVLMNWLPT 265
             LLP    +K+  ++  +     +IPW+ IL   PVWA++  +F  ++  ++L+ W+PT
Sbjct: 187 PTLLP--EERKLIADNCASKEPVKSIPWRLILSKPPVWALISCHFCHNWGTFILLTWMPT 246

Query: 266 YFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIAADYLVTKRILSVTRTRKFLNTTGFSV 325
           Y+   L+ +L E G   + P++ M + +N GG  AD LV+ R  SVT  RK + T GF  
Sbjct: 247 YYHQVLKFNLMESGLLSVFPWMTMAISANAGGWIADTLVS-RGFSVTNVRKIMQTIGFLG 306

Query: 326 ASMALMAIPIFRTSSGAIFCSSVALGFLALGRAGFAVNHMDIAPRYAGIVMGVSNTAGTL 385
            +  L  +    + + A+ C + + G  A  ++G   NH DIAPRY+G+++G+SNTAG L
Sbjct: 307 PAFFLTQLKHIDSPTMAVLCMACSQGTDAFSQSGLYSNHQDIAPRYSGVLLGLSNTAGVL 366

Query: 386 AGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFSIPGLLCVLSSIVFLLFSTGERIFD 443
           AG++G   TG +L+             SW  VF+I   L ++ ++++ LFSTGE+I D
Sbjct: 367 AGVLGTAATGHILQHG-----------SWDDVFTISVGLYLVGTVIWNLFSTGEKIID 400

BLAST of CmaCh16G002490 vs. TAIR 10
Match: AT2G38060.1 (phosphate transporter 4;2 )

HSP 1 Score: 210.3 bits (534), Expect = 3.1e-54
Identity = 137/443 (30.93%), Postives = 225/443 (50.79%), Query Frame = 0

Query: 6   IPKRYWIVLLTFICTSVCYVERVGFSIAYTFAADAVGVDQSSKGTILSTFYYGYACSQVP 65
           +P+R  +V+LT     +C  +RV  S+A    AD +G   S  G + S+F +GY  S V 
Sbjct: 95  MPERIKVVILTACMMCLCNADRVVMSVAVVPLADKLGWSSSFLGVVQSSFLWGYIFSSVI 154

Query: 66  GGWAAQKIGGRRVLLISFILWSLTCALVPLDPSR-VMVLVIARLLVGVAQGFIFPSIHTV 125
           GG    + GG+RVL     LWSL   L P   +   + L+  R   G+A+G   PS+ T+
Sbjct: 155 GGALVDRYGGKRVLAWGVALWSLATLLTPWAAAHSTLALLCVRAFFGLAEGVAMPSMTTL 214

Query: 126 LAQWVPPHERSRSVSLTTSGMYLGAAAGMLILPSLVKYRGPQSVFAAEALLGATWSVLWL 185
           L++W P  ER+ +V ++ +G ++G   G+L+ P ++   G    F   A LG  W   W 
Sbjct: 215 LSRWFPMDERASAVGISMAGFHMGNVVGLLLTPLMLSSIGISGPFILFASLGLLWVSTWS 274

Query: 186 KYATDPPRSEHPKAAAAGFGEPLLPIKGNQKVRVESGGTSTRTSNI-----PWKKILLS- 245
              T+ P+                P     ++R+   G   + S I     P  ++LLS 
Sbjct: 275 SGVTNNPQDS--------------PFITRSELRLIQAGKPVQPSTISPKPNPSLRLLLSK 334

Query: 246 LPVWAIVVNNFTFHYALYVLMNWLPTYFELGLQLSLQEMGSSKMLPYLNMFLFSNVGGIA 305
           LP WAI+  N T ++  +VL++W+P YF+    ++L++      LP+  M +     G A
Sbjct: 335 LPTWAIIFANVTNNWGYFVLLSWMPVYFQTVFNVNLKQAAWFSALPWATMAISGYYAGAA 394

Query: 306 ADYLVTKRILSVTRTRKFLNTTGFSVASMALMAIPIFRTSSGAIFCSSVALGFLALGRAG 365
           +D+L+ +   SVT  RK + + GF    ++L+ +   ++ S A    ++AL   +  +AG
Sbjct: 395 SDFLI-RTGHSVTSVRKIMQSIGFMGPGLSLLCLNFAKSPSCAAVFMTIALSLSSFSQAG 454

Query: 366 FAVNHMDIAPRYAGIVMGVSNTAGTLAGIIGVDFTGKLLEAAKAANSDLSSPESWRAVFS 425
           F +N  DIAP+YAG + G+SN AGTLA I+    TG  ++             S++A  +
Sbjct: 455 FLLNMQDIAPQYAGFLHGISNCAGTLAAIVSTIGTGYFVQWL----------GSFQAFLT 512

Query: 426 IPGLLCVLSSIVFLLFSTGERIF 442
           +   L   +++ +LLF+TGER+F
Sbjct: 515 VTAFLYFATTVFWLLFATGERVF 512

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKV12.1e-19678.51Probable anion transporter 5 OS=Arabidopsis thaliana OX=3702 GN=ANTR5 PE=2 SV=1[more]
Q53P546.4e-18572.05Probable anion transporter 6 OS=Oryza sativa subsp. japonica OX=39947 GN=PHT4;6 ... [more]
Q2QWW73.2e-18470.23Probable anion transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=PHT4;7 ... [more]
Q652N58.0e-7135.37Probable anion transporter 4, chloroplastic OS=Oryza sativa subsp. japonica OX=3... [more]
Q8GX781.2e-6934.92Ascorbate transporter, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PHT4;4 P... [more]
Match NameE-valueIdentityDescription
A0A6J1J9U73.9e-246100.00probable anion transporter 5 OS=Cucurbita maxima OX=3661 GN=LOC111482603 PE=4 SV... [more]
A0A6J1E3G48.2e-24498.87probable anion transporter 5 OS=Cucurbita moschata OX=3662 GN=LOC111430488 PE=4 ... [more]
A0A1S4E1C91.4e-23896.15probable anion transporter 5 OS=Cucumis melo OX=3656 GN=LOC103495770 PE=4 SV=1[more]
A0A5D3CTQ21.4e-23896.15Putative anion transporter 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0KVQ12.3e-23896.15MFS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G107370 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_022984219.18.1e-246100.00probable anion transporter 5 [Cucurbita maxima] >XP_022984220.1 probable anion t... [more]
XP_022922505.11.7e-24398.87probable anion transporter 5 [Cucurbita moschata] >XP_022922506.1 probable anion... [more]
KAG7014888.14.2e-24298.86putative anion transporter 5, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_008455652.12.8e-23896.15PREDICTED: probable anion transporter 5 [Cucumis melo] >XP_008455654.1 PREDICTED... [more]
XP_004137171.14.8e-23896.15probable anion transporter 5 [Cucumis sativus] >XP_031740473.1 probable anion tr... [more]
Match NameE-valueIdentityDescription
AT5G44370.11.5e-19778.51phosphate transporter 4;6 [more]
AT4G00370.18.2e-7134.92Major facilitator superfamily protein [more]
AT2G29650.17.7e-6935.31phosphate transporter 4;1 [more]
AT2G29650.32.0e-6434.93phosphate transporter 4;1 [more]
AT2G38060.13.1e-5430.93phosphate transporter 4;2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 7..198
e-value: 2.1E-41
score: 143.6
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 238..439
e-value: 1.5E-14
score: 55.8
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 7..435
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 17..389
e-value: 3.7E-52
score: 177.4
NoneNo IPR availablePANTHERPTHR11662SOLUTE CARRIER FAMILY 17coord: 1..442
NoneNo IPR availablePANTHERPTHR11662:SF435PHOSPHATE TRANSPORTER 4,6-RELATEDcoord: 1..442
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 1..439
score: 22.436903
IPR044777Solute carrier family 17 member 9-likeCDDcd17380MFS_SLC17A9_likecoord: 13..435
e-value: 1.94371E-133
score: 386.525

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G002490.1CmaCh16G002490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity