CmaCh16G002270 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G002270
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCma_Chr16: 1043093 .. 1044817 (-)
RNA-Seq ExpressionCmaCh16G002270
SyntenyCmaCh16G002270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTTCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCAGAGACCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTGAAAGGTCCCTCGCCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTTTTTCAGGTAAAGTTTCAATTCTTTACACTGATTCTAGAAACTGGAGAGATTTATATTAGCTACCTATTAACTTCAATCTGTTTTTCTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAGCCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTAGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACATACTTATAGGTAACTCAATATTTTAATTCTCCCTCTCCACTTCTGACCCAATTTCCATTCTTTTTCTTTTCCTGATTACAATGCAAAATTTACTTACATGTGAAGGCTGTACAATGAAATTCAACAGAAAGATACAATAAAACCATTGGACCGCCAAAGAGATCACCGCTATGCATATGCTGACGCCTATGAACCAGAACTTCAGCTAACAATGTTACTTTTAATTATGCTAACATTGAGTATGGTCTAAAAGTTTGGACTCTTTTTATGCATACTCAGCATACTTGAGCATTCTTCTATAATGAACCATAAATATAGGACTTGAATTGCACTCACCAATAAATGACAATCAAAACAGGACAGAAAGCACTACTTACCGACAAAAATATGGACATCTCTGATTAAAATATCAATATAAAAATCACCCCCCCCAAAAAAAAACTATTATTTAATCTCTTACTTTGAGAAAACCACATCAGTTAAAATTTTATAAGATCCTAGGTGCATATTTTCTTCAGAGATATGTCCTTGTGTTTTACGTGTATATATATACATATTATATATATGTATGTGTATGACCGTTTCAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTAAGCTTAGAGATAGAGGAAGCCATAACTGAAAGCTTGGGTTTAAGACCATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGCTGCATAACCACAATTCTACAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTAAGCAATGGGCTTTACAAGGGTGTCGTCCACAGAGCCATTGTCAATTCTGAGAGAACAAGAATCTCCATTTCCAGTCTACACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATTAAGTATAAAGAAAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAAAGCTTCATCGACACACTCAAGATCCAGAATTAA

mRNA sequence

ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTTCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCAGAGACCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTGAAAGGTCCCTCGCCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTTTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAGCCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTAGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACATACTTATAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTAAGCTTAGAGATAGAGGAAGCCATAACTGAAAGCTTGGGTTTAAGACCATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGCTGCATAACCACAATTCTACAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTAAGCAATGGGCTTTACAAGGGTGTCGTCCACAGAGCCATTGTCAATTCTGAGAGAACAAGAATCTCCATTTCCAGTCTACACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATTAAGTATAAAGAAAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAAAGCTTCATCGACACACTCAAGATCCAGAATTAA

Coding sequence (CDS)

ATGGGGCTAAGAGGTGAGAGTTCAGGGTCATTTTCCACAGGACAGACTGCACAAGAGAAAGGCTTAAACTATGTCCCTAATGCTTATGTGATTCCTACCCCACAGCGCCCGAGTTTGTCCCCAGAGACCGCCATTGTTCCCATCATTGATATGGCTTCTCTAAGATCAAATGATTCAGCTGAAAGGTCCCTCGCCATTGAAGCGCTCAGAAAAGCTTGCATTAGCCTCGGCTTTTTTCAGATAATCAATCATGGAATCAGCAAAACGGTGATGGAGGAAGCACTAAGCCAAGCCAGGGAGTTCTTTAACTTACCACTAAAGGAGAAGATGAAGTATAAGTCAGATGATGTTAGCAAAGCAGTCAGGTATGGAACAAGCTTGAAAGATGGAGTAGACAAAATAAAGTTTTGGAGAGTTTTCCTCAAACACTATGCTCATCCATTGGAAGATTGGGTAGACTCCTGGCCCACCAATCCACATACTTATAGGGAAAAGATGGGACAATACACCAAGGAGGTGAGAAAAGTAAGCTTAGAGATAGAGGAAGCCATAACTGAAAGCTTGGGTTTAAGACCATACCTCAGCAGCAAAATGGCTGAAGGAGTCCAGGTAGTGGCAGTAAACTGCTACCCGCCATGTCCACAGCCAGGGGTCGCACTTGGGTTGCCACCACATACAGACTACAGCTGCATAACCACAATTCTACAAAGCAGCCAGGGACTTGAAATCATGGACCGGGCTGATGGGAAATGGAAGATGGTTCCTAATGTTGATGGCAGTCTGCAGGTCCATGTGGGCGATCATGTGGAGGTTCTAAGCAATGGGCTTTACAAGGGTGTCGTCCACAGAGCCATTGTCAATTCTGAGAGAACAAGAATCTCCATTTCCAGTCTACACAGCTTGGGAATGGACGAGAAAATGAAGCCTGCAGAAGAGCTTGTGGATGAACAAAATCCTATTAAGTATAAAGAAAGCAGCTTCAATGATTTTCTCAAATTTCTCTCCAGCAACGATCTTGCAGAAGGAAAAAGCTTCATCGACACACTCAAGATCCAGAATTAA

Protein sequence

MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSAERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN
Homology
BLAST of CmaCh16G002270 vs. ExPASy Swiss-Prot
Match: Q7XR84 (Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.5e-83
Identity = 154/322 (47.83%), Postives = 217/322 (67.39%), Query Frame = 0

Query: 31  IPTPQRPSLSPETAIVPIIDMASLRSNDSAERSLAIEALRKACISLGFFQIINHGISKTV 90
           IP  Q PS       +P+I++  L  +D   RS  +  + KAC  LG+FQ+I+HGIS++V
Sbjct: 8   IPQEQLPSQELHPPPMPVINLGHLSLDDPTVRSRVVNDIAKACRDLGYFQVISHGISQSV 67

Query: 91  MEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLED 150
           M+ A+  A EFF LP + K +Y SDD+ + VRY TS KDG   I   R FLKHYAHPL D
Sbjct: 68  MDGAIEAASEFFKLPNEIKKEYASDDIRQPVRYDTSSKDG---ISMSRAFLKHYAHPLCD 127

Query: 151 WVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGL-RPYLSSKMAEGVQVVAVNC 210
           W+  WP  P  YRE M +Y  EVR V+L++ EAI E LG+ + Y+  K  EG+Q+++VNC
Sbjct: 128 WLQYWPQQPPIYREYMAKYAVEVRVVALKLMEAILEGLGIGKEYMQEKFEEGLQLLSVNC 187

Query: 211 YPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHV 270
           YP   Q   ++GL  H+DY  +T +L S QGLE++DR+   WK+V  +  +L VHVGDH+
Sbjct: 188 YPKVSQSDTSIGLAAHSDYGLLTILLTSCQGLEVVDRSSNSWKVVQQLPHALHVHVGDHM 247

Query: 271 EVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFND 330
           EVLSNG  K VVHRA++N +  RIS++S+H   + EK+  A+ELVDE+NP KYKE+SFND
Sbjct: 248 EVLSNGRIKTVVHRAVLNPQEARISLASIHGFALHEKVSSAKELVDEENPQKYKENSFND 307

Query: 331 FLKFLSSNDLAEGKSFIDTLKI 352
           FL+ L++N     ++F+++L++
Sbjct: 308 FLEHLTANMDNRQRNFLESLRM 326

BLAST of CmaCh16G002270 vs. ExPASy Swiss-Prot
Match: Q7XR83 (2-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=2ODD21 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.9e-79
Identity = 154/317 (48.58%), Postives = 212/317 (66.88%), Query Frame = 0

Query: 37  PSLSPETAIVPIIDMASLRSNDSAERSLAIEALRKACISLGFFQIINHGISKTVMEEALS 96
           P L P    +P+I++  L + DSA R+LA+  +  AC   G F+++NHGIS++ M  AL 
Sbjct: 19  PPLPPPPRPLPVINLGRL-TMDSASRALAVRDIVLACRERGCFEVVNHGISRSCMNGALE 78

Query: 97  QAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWP 156
            A EFF L  + K ++ SDD+ + +RY TS +DG   I   R FLKHYA+PL+DW+  WP
Sbjct: 79  AASEFFQLSTERKEEFASDDIRQPIRYDTSSRDG---ISMSRSFLKHYANPLDDWIKFWP 138

Query: 157 TNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYP-PCP 216
           T P TYREKMG+Y  E ++VS+++ EAI + LGL P YL  K+  GVQ VA+N YP    
Sbjct: 139 TQPPTYREKMGEYAVETQRVSMQLMEAILQGLGLGPSYLQEKLEGGVQFVALNNYPQSSA 198

Query: 217 QPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSN 276
           +    +GL PH+DY  +T +LQSS GLE+M   D  W  VP + G+L VHVGDH+EVLSN
Sbjct: 199 KKADKIGLAPHSDYGFLTILLQSSPGLEVMHHEDDAWTSVPAIPGALHVHVGDHLEVLSN 258

Query: 277 GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL 336
           G  K +VHRA++N   +RISI+S+H L MDE++  AEELVDE +P  Y+ SSF DFL FL
Sbjct: 259 GQLKSLVHRAVLNPNESRISIASIHGLSMDEEVHCAEELVDEHHPKMYRGSSFQDFLDFL 318

Query: 337 SSNDLAEGKSFIDTLKI 352
            +N +   K ++++L+I
Sbjct: 319 PAN-MNRYKRYVESLRI 330

BLAST of CmaCh16G002270 vs. ExPASy Swiss-Prot
Match: Q6YYX9 (Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC1 PE=2 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.9e-65
Identity = 134/342 (39.18%), Postives = 200/342 (58.48%), Query Frame = 0

Query: 19  EKGLNYVPNAYVIPTPQRPSLSPETAI-------VPIIDMASLRSNDSAERSLAIEALRK 78
           + G+  +P  YV+P   RP  +   A        +P++D++ LR    +ER   +  L  
Sbjct: 46  DSGITRLPGNYVLPASDRPGQAAGAAAAAGGSVKLPVVDLSRLRV--PSERGAVLRTLDA 105

Query: 79  ACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGV 138
           AC   GFFQ++NHG+   V+   L  AR FF LP  E+ +Y S DV   VRYGTS     
Sbjct: 106 ACREYGFFQVVNHGVGGEVVGGMLDVARRFFELPQPERERYMSADVRAPVRYGTSFNQVR 165

Query: 139 DKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGL- 198
           D +  WR FLK    PL   V+SWPT+P   RE   +Y +  ++V +E+ EA  E+LG+ 
Sbjct: 166 DAVLCWRDFLKLACMPLAAVVESWPTSPADLREVASRYAEANQRVFMEVMEAALEALGVG 225

Query: 199 RPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSS-QGLEIMDRADG 258
              +   +A G Q++ VNCYP CPQP + LG+PPH+DY  +T +LQ    GL++M    G
Sbjct: 226 GGGVMEDLAAGTQMMTVNCYPECPQPELTLGMPPHSDYGFLTLVLQDEVAGLQVMHA--G 285

Query: 259 KWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKP 318
           +W  V  + GS  V+VGDH+E+LSNG Y+ V+HR  VNS R R+S++S HS+  +  + P
Sbjct: 286 EWLTVDPLPGSFVVNVGDHLEILSNGRYRSVLHRVKVNSRRLRVSVASFHSVAPERVVSP 345

Query: 319 AEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKI 352
           A EL+D+++P +Y ++    FL +L+S      KSF+ + ++
Sbjct: 346 APELIDDRHPRRYMDTDLATFLAYLAS-AAGNHKSFLHSRRL 382

BLAST of CmaCh16G002270 vs. ExPASy Swiss-Prot
Match: Q651B6 (Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japonica OX=39947 GN=SLC2 PE=2 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 2.3e-60
Identity = 142/363 (39.12%), Postives = 211/363 (58.13%), Query Frame = 0

Query: 5   GESSGS--FSTG-QTAQEKGLNYVPNAYVIPTPQRPS--LSPETAIVPIIDMASLRSNDS 64
           GE SGS  +  G +   + G+  +P  YV+P   RP+  ++P  A++P++D+A+LR+ D 
Sbjct: 13  GEGSGSSEWELGVRQLCDSGITTLPARYVLPPADRPARYVTP-PALLPVVDLAALRARDP 72

Query: 65  AERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSK 124
            +    + AL  AC   GFFQ++NHG+    M    +  R FF+LPL  + +Y S D+  
Sbjct: 73  CQ----LAALHAACRDYGFFQLLNHGVPPDAM--LYAARRFFFDLPLPARKRYMSADIRA 132

Query: 125 AVRYGTSLKDGVDKIKFWRVFLK---HYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKV 184
           AVRYGTS     D +  WR FLK        L D + SWP  P   R     Y    +++
Sbjct: 133 AVRYGTSFNQLNDAVLSWRDFLKLLIRDTRRLADVLPSWPDAPDDLRPAAAAYATACQRL 192

Query: 185 SLEIEEAITESLGL---RPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT 244
             E+ EA  ++LG+   R  L  +   G Q++ VNC+P CP+P + LG+PPH+DY  +T 
Sbjct: 193 FRELMEAALDALGIVRCRRQLLEECDAGSQMMMVNCFPACPEPELTLGMPPHSDYGLLTI 252

Query: 245 ILQSS-QGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTR 304
           +LQ   +GLE+     G W +V  + G++ V+VGDH+E+LSNGLY+ V+HR  VN  R R
Sbjct: 253 LLQDEVRGLEVSYGDGGGWAVVEPLPGAVVVNVGDHLEILSNGLYRSVLHRVRVNGRRAR 312

Query: 305 ISISSLHSLGMDEKMKPAEELVDEQ--NPIKYKESSFNDFLKFLSSNDLAEG--KSFIDT 352
           +S++SLHSL  +  + PA ELVDEQ   P +Y ++    FL +L+S   AEG  KSF+ +
Sbjct: 313 VSVASLHSLAAERVIGPAAELVDEQRGRPRRYMDTDMAAFLAYLAS---AEGNHKSFLHS 365

BLAST of CmaCh16G002270 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.7e-60
Identity = 125/324 (38.58%), Postives = 201/324 (62.04%), Query Frame = 0

Query: 25  VPNAYVIPTPQRPSLSPETAI--VPIIDMASLRSNDSAERSLAIEALRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S +RS  I+ + +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +S AREFF++ ++EKMK  SDD +K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGL-RPYLSSKMAEG 204
            + +P+  +V+ WP+NP +++E + +Y++EVR+V  +IEE I+ESLGL + Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ--GLEIMDRADGKWKMVPNVDG 264
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG+W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQIL--IDGQWFAVNPHPD 254

Query: 265 SLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEEL----VD 324
           +  +++GD ++ LSNG+YK V HRA+ N+E  R+S++S         M PA+ L     D
Sbjct: 255 AFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDD 314

Query: 325 EQNPIKYKESSFNDFLKFLSSNDL 340
           E  P+ YK+ ++ ++ K   S +L
Sbjct: 315 ETKPV-YKDFTYAEYYKKFWSRNL 329

BLAST of CmaCh16G002270 vs. ExPASy TrEMBL
Match: A0A6J1JAE6 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LOC111482715 PE=3 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 4.3e-195
Identity = 353/381 (92.65%), Postives = 353/381 (92.65%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  ERSLAIEALRKACISLGFF----------------------------QIINHGISKTVME 120
           ERSLAIEALRKACISLGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180
           EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240
           DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300
           CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL
Sbjct: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300

Query: 301 SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK 354
           SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK 360

BLAST of CmaCh16G002270 vs. ExPASy TrEMBL
Match: A0A6J1E3T8 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=LOC111430549 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 5.8e-192
Identity = 347/376 (92.29%), Postives = 352/376 (93.62%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL+GESSGSF+TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  ERSLAIEALRKACISLGFF----------------------QIINHGISKTVMEEALSQA 120
           +RSL IEALRKACISLGFF                      QIINHGISKTVMEEAL+QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180
           REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPG 240
           PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP YLSSKMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 354
           KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of CmaCh16G002270 vs. ExPASy TrEMBL
Match: A0A5A7TMH3 (Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00340 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.9e-177
Identity = 313/354 (88.42%), Postives = 330/354 (93.22%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GE SG+F TG+TAQEKGL+YVPNAYVIP P RPSLSPE AIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  ERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKA 120
           +RSLA+E LRKACISLGFFQIINHGI++TVMEEALSQA EFFNLPL EKMKYKSDDV KA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEI 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDW+DSWPTNP  YREKMGQYTKEVRKVSLEI
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 EEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
            EAITESL L P YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLH 300
           GLEIMD ADG WKMV  +DG+LQVHVGDHVEVLSNGLYKGVVHRA VNSERTRISI+SLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           SLGMDEKMKPAEELV+EQNP KYKESSFNDFLKFLSSNDLAEG SFI+TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of CmaCh16G002270 vs. ExPASy TrEMBL
Match: A0A1S3BX86 (protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 6.9e-177
Identity = 313/354 (88.42%), Postives = 330/354 (93.22%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GE SG+F TG+TAQEKGL+YVPNAYVIP P RPSLSPE AIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  ERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKA 120
           +RSLA+E LRKACISLGFFQIINHGI++TVMEEALSQA EFFNLPL EKMKYKSDDV KA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEI 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDW+DSWPTNP  YREKMGQYTKEVRKVSLEI
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 EEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
            EAITESL L P YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLH 300
           GLEIMD ADG WKMV  +DG+LQVHVGDHVEVLSNGLYKGVVHRA VNSERTRISI+SLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           SLGMDEKMKPAEELV+EQNP KYKESSFNDFLKFLSSNDLAEG SFI+TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of CmaCh16G002270 vs. ExPASy TrEMBL
Match: A0A6J1CIV6 (flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV=1)

HSP 1 Score: 590.9 bits (1522), Expect = 3.6e-165
Identity = 302/380 (79.47%), Postives = 325/380 (85.53%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSGSF TG++AQEKGL+YVPNAYVIP P R S S ETAI+PIIDMASLRS DSA
Sbjct: 1   MGLGGESSGSFPTGESAQEKGLSYVPNAYVIPAPHRSSSSRETAIIPIIDMASLRSTDSA 60

Query: 61  ERSLAIEALRKACISLGFF---------------------------QIINHGISKTVMEE 120
           +RSLAIE LR+ACI LGFF                           QIINH I++ VMEE
Sbjct: 61  QRSLAIEELREACIRLGFFQVSFNASILHSDPEKICITSLTSVCFVQIINHRINERVMEE 120

Query: 121 ALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVD 180
           AL QA EFF+LPLKEKMKYKSDDVS+AVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+ 
Sbjct: 121 ALEQAYEFFDLPLKEKMKYKSDDVSRAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIH 180

Query: 181 SWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYPP 240
           SWPT+P +YREKMG YT+EVRKV LE+ EAITESLGL P YLSSKMAEGVQVVAVNCYPP
Sbjct: 181 SWPTSPRSYREKMGTYTREVRKVGLEVMEAITESLGLCPTYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300
           CPQP VALGLPPHTDYSCITTILQSS+GLEIMD ADG WK+VPN+DG+LQVH+GDHVEVL
Sbjct: 241 CPQPEVALGLPPHTDYSCITTILQSSEGLEIMDMADGTWKLVPNIDGALQVHLGDHVEVL 300

Query: 301 SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK 353
           SNGLYKGVVHRA VNSE TRISI+SLHSLGMDEKMKPAEELVD+QNP KYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRATVNSESTRISITSLHSLGMDEKMKPAEELVDQQNPKKYKESSFNDFLK 360

BLAST of CmaCh16G002270 vs. NCBI nr
Match: XP_022984408.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita maxima])

HSP 1 Score: 690.3 bits (1780), Expect = 8.9e-195
Identity = 353/381 (92.65%), Postives = 353/381 (92.65%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  ERSLAIEALRKACISLGFF----------------------------QIINHGISKTVME 120
           ERSLAIEALRKACISLGFF                            QIINHGISKTVME
Sbjct: 61  ERSLAIEALRKACISLGFFQVKFQFFTLILETGEIYISYLLTSICFLQIINHGISKTVME 120

Query: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180
           EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV
Sbjct: 121 EALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWV 180

Query: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240
           DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP
Sbjct: 181 DSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPYLSSKMAEGVQVVAVNCYPP 240

Query: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300
           CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL
Sbjct: 241 CPQPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVL 300

Query: 301 SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK 354
           SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK
Sbjct: 301 SNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLK 360

BLAST of CmaCh16G002270 vs. NCBI nr
Match: XP_022922584.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata])

HSP 1 Score: 679.9 bits (1753), Expect = 1.2e-191
Identity = 347/376 (92.29%), Postives = 352/376 (93.62%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL+GESSGSF+TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA
Sbjct: 1   MGLKGESSGSFTTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60

Query: 61  ERSLAIEALRKACISLGFF----------------------QIINHGISKTVMEEALSQA 120
           +RSL IEALRKACISLGFF                      QIINHGISKTVMEEAL+QA
Sbjct: 61  QRSLTIEALRKACISLGFFQVKFQATYLFYISXLLTSICFLQIINHGISKTVMEEALNQA 120

Query: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180
           REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN
Sbjct: 121 REFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTN 180

Query: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPG 240
           PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP YLSSKMAEGVQVVAVNCYPPCPQPG
Sbjct: 181 PHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCPQPG 240

Query: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300
           VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY
Sbjct: 241 VALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLY 300

Query: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 354
           KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN
Sbjct: 301 KGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSN 360

BLAST of CmaCh16G002270 vs. NCBI nr
Match: XP_023552054.1 (LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 672.2 bits (1733), Expect = 2.5e-189
Identity = 345/379 (91.03%), Postives = 349/379 (92.08%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSGSF TGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRS+DSA
Sbjct: 62  MGLGGESSGSFPTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSDDSA 121

Query: 61  ERSLAIEALRKACISLGFF-------------------------QIINHGISKTVMEEAL 120
           +RSLAIE LRKACIS+GFF                         QIINHGISKTVMEEAL
Sbjct: 122 QRSLAIEVLRKACISIGFFQVKFQATYLPTSFILASSXLQFVFLQIINHGISKTVMEEAL 181

Query: 121 SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 180
           SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW
Sbjct: 182 SQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSW 241

Query: 181 PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCP 240
           PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRP YLSSKMAEGVQVVAVNCYPPCP
Sbjct: 242 PTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGLRPTYLSSKMAEGVQVVAVNCYPPCP 301

Query: 241 QPGVALGLPPHTDYSCITTILQSSQGLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSN 300
           QPGVALGLPPHTDYSCITTILQSSQGLEIMD ADGKWKMVPNVDGSLQVHVGDHVEVLSN
Sbjct: 302 QPGVALGLPPHTDYSCITTILQSSQGLEIMDPADGKWKMVPNVDGSLQVHVGDHVEVLSN 361

Query: 301 GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL 354
           GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL
Sbjct: 362 GLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFL 421

BLAST of CmaCh16G002270 vs. NCBI nr
Match: XP_008453850.1 (PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa] >TYK16900.1 protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo var. makuwa])

HSP 1 Score: 629.8 bits (1623), Expect = 1.4e-176
Identity = 313/354 (88.42%), Postives = 330/354 (93.22%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GE SG+F TG+TAQEKGL+YVPNAYVIP P RPSLSPE AIVPIIDMASLRSNDS 
Sbjct: 1   MGLNGEGSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSLSPEIAIVPIIDMASLRSNDSV 60

Query: 61  ERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKA 120
           +RSLA+E LRKACISLGFFQIINHGI++TVMEEALSQA EFFNLPL EKMKYKSDDV KA
Sbjct: 61  QRSLAMEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLTEKMKYKSDDVCKA 120

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEI 180
           VRYGTSLKDG+DKIKFWRVFLKHYAHPLEDW+DSWPTNP  YREKMGQYTKEVRKVSLEI
Sbjct: 121 VRYGTSLKDGIDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGQYTKEVRKVSLEI 180

Query: 181 EEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
            EAITESL L P YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQ+SQ
Sbjct: 181 MEAITESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQNSQ 240

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLH 300
           GLEIMD ADG WKMV  +DG+LQVHVGDHVEVLSNGLYKGVVHRA VNSERTRISI+SLH
Sbjct: 241 GLEIMDPADGTWKMVQKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 300

Query: 301 SLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           SLGMDEKMKPAEELV+EQNP KYKESSFNDFLKFLSSNDLAEG SFI+TLKIQ+
Sbjct: 301 SLGMDEKMKPAEELVNEQNPKKYKESSFNDFLKFLSSNDLAEGNSFINTLKIQD 354

BLAST of CmaCh16G002270 vs. NCBI nr
Match: XP_004146935.2 (flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa_014422 [Cucumis sativus])

HSP 1 Score: 628.2 bits (1619), Expect = 4.2e-176
Identity = 310/354 (87.57%), Postives = 331/354 (93.50%), Query Frame = 0

Query: 1   MGLRGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSA 60
           MGL GESSG+F TG+TAQEKGL+YVPNAYVIP P RPS+SPE AIVPIIDMASLRS+DS 
Sbjct: 3   MGLNGESSGTFPTGKTAQEKGLSYVPNAYVIPAPHRPSMSPEIAIVPIIDMASLRSSDSV 62

Query: 61  ERSLAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKA 120
           +RSLA E LRKACISLGFFQIINHGI++TVMEEALSQA EFFNLPLKEKMKYKSDDV KA
Sbjct: 63  QRSLATEELRKACISLGFFQIINHGITETVMEEALSQANEFFNLPLKEKMKYKSDDVCKA 122

Query: 121 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEI 180
           VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDW+DSWPTNP  YREKMG+YTKEVRKVSLEI
Sbjct: 123 VRYGTSLKDGVDKIKFWRVFLKHYAHPLEDWIDSWPTNPRNYREKMGRYTKEVRKVSLEI 182

Query: 181 EEAITESLGLRP-YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ 240
            EAI ESL L P YLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITT+LQSSQ
Sbjct: 183 MEAINESLNLSPTYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTVLQSSQ 242

Query: 241 GLEIMDRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLH 300
           GLE+MD ADG WKMVP +DG+LQVHVGDHVEVLSNGLYKGVVHRA VNSERTRISI+SLH
Sbjct: 243 GLEVMDPADGTWKMVPKIDGALQVHVGDHVEVLSNGLYKGVVHRATVNSERTRISITSLH 302

Query: 301 SLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           SLGMDEKMKPAEELV+E NP KYK+SSFNDFLKFLSSNDLAEGKSF++TLK+Q+
Sbjct: 303 SLGMDEKMKPAEELVNEHNPKKYKDSSFNDFLKFLSSNDLAEGKSFLNTLKLQD 356

BLAST of CmaCh16G002270 vs. TAIR 10
Match: AT5G07480.1 (KAR-UP oxidoreductase 1 )

HSP 1 Score: 394.4 bits (1012), Expect = 9.4e-110
Identity = 195/356 (54.78%), Postives = 254/356 (71.35%), Query Frame = 0

Query: 4   RGESSGSFSTGQTAQEKGLNYVPNAYVIPTPQRPSLSPETAIVPIIDMASLRSNDSAERS 63
           +GE S +F  G +AQE+ L YVP+ YV+P   +P  S  + IVP ID++ L+  D   R 
Sbjct: 3   KGEDSSTFKMGNSAQERSLPYVPDCYVVPPSSKPCDS-NSGIVPTIDVSRLKGGDDERRG 62

Query: 64  LAIEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRY 123
                            I+NHGI++ ++++AL  A  FF LP KEK ++ S+DV   VRY
Sbjct: 63  -----------------IVNHGINQNILDDALEVANSFFELPAKEKKQFMSNDVYAPVRY 122

Query: 124 GTSLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEA 183
            TSLKDG+D I+FWR+FLKHYAHPL  W+  WP NP  YREKMG++ +EVRK+S+E+  A
Sbjct: 123 STSLKDGLDTIQFWRIFLKHYAHPLHRWIHLWPENPPGYREKMGKFCEEVRKLSIELMGA 182

Query: 184 ITESLGL-RPYLSSKMAE-GVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGL 243
           ITESLGL R YLSS+M E G+QV+ VNCYPPCP P  ALGLPPH+DYSCIT +LQ+  GL
Sbjct: 183 ITESLGLGRDYLSSRMDENGMQVMTVNCYPPCPDPETALGLPPHSDYSCITLLLQNLDGL 242

Query: 244 EIMD----RADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISS 303
           +I D       G+W  VP V G L+VH+GDHVEVLSNGLYK +VH+  +N E+TRIS++S
Sbjct: 243 KIFDPMAHGGSGRWVGVPQVTGVLKVHIGDHVEVLSNGLYKSIVHKVTLNEEKTRISLAS 302

Query: 304 LHSLGMDEKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           LHSLGMD+KM    ELV+++NP++YKESSFNDFL FL  ND+++G  FIDTL+I++
Sbjct: 303 LHSLGMDDKMSVPRELVNDENPVRYKESSFNDFLDFLVKNDISQGDRFIDTLRIKD 340

BLAST of CmaCh16G002270 vs. TAIR 10
Match: AT3G60290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 5.2e-92
Identity = 165/348 (47.41%), Postives = 233/348 (66.95%), Query Frame = 0

Query: 11  FSTGQTAQEKGLNYVPNAYVIPTPQRPSLSP----ETAIVPIIDMASLRSNDSAERSLAI 70
           F++     E G  +VP  Y++P  QRP L P     T  +P+ID++ L  +D   R   I
Sbjct: 13  FTSAMELTESGDPHVPTRYILPPSQRPMLGPSIGTSTINLPVIDLSFL--HDPLLRLCVI 72

Query: 71  EALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTS 130
             +  AC   GFFQ+INHGIS  V+++A   A  FF+LP  EKM   SD+  + VRYGTS
Sbjct: 73  HEIELACKGFGFFQVINHGISSAVVKDAQDSATRFFDLPADEKMHLVSDNFQEPVRYGTS 132

Query: 131 LKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITE 190
           +    D++ +WR F+KHY+HPL +W++ WP+NP  Y+EK+G+Y +    +  ++ EAI+E
Sbjct: 133 INHSTDRVHYWRDFIKHYSHPLSNWINLWPSNPPCYKEKVGKYAEATHVLHKQLIEAISE 192

Query: 191 SLGL-RPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIMD 250
           SLGL + YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+DY  +T +LQSS+GL+I D
Sbjct: 193 SLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEIALGMPPHSDYGSLTILLQSSEGLQIKD 252

Query: 251 RADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDE 310
             +  W  VP ++G+L V +GD VEV+SNG+YK VVHR  VN +  R+S +SLHSL M +
Sbjct: 253 -CNNNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVVHRVTVNKDYKRLSFASLHSLPMHK 312

Query: 311 KMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           K+ PA +LV+E  P  Y E SFNDFL ++S ND+ + K FIDTL+ +N
Sbjct: 313 KISPATQLVNENKPAAYGEFSFNDFLDYISRNDITQ-KRFIDTLRKKN 356

BLAST of CmaCh16G002270 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 6.8e-92
Identity = 167/346 (48.27%), Postives = 237/346 (68.50%), Query Frame = 0

Query: 10  SFSTGQTAQEKGLNYVPNAYVIPTPQRP----SLSPETAIVPIIDMASLRSNDSAERSLA 69
           SF++  T    G+  VP+ YV+P  QRP    SL      +P+ID++ L  +    RSLA
Sbjct: 12  SFTSAMTLTNSGVPQVPDRYVLPPSQRPALGSSLGTSETTLPVIDLSLL--HQPFLRSLA 71

Query: 70  IEALRKACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGT 129
           I  +  AC   GFFQ+INHGI  +V+ +AL  A +FF+LP++EKM   S +V + VRYGT
Sbjct: 72  IHEISMACKEFGFFQVINHGIPSSVVNDALDAATQFFDLPVEEKMLLVSANVHEPVRYGT 131

Query: 130 SLKDGVDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAIT 189
           SL    D++ +WR F+KHY+HPL  W+D WP+NP  Y++K+G+Y +    +  ++ EAI+
Sbjct: 132 SLNHSTDRVHYWRDFIKHYSHPLSKWIDMWPSNPPCYKDKVGKYAEATHLLHKQLIEAIS 191

Query: 190 ESLGL-RPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQGLEIM 249
           ESLGL + YL  ++ EG QV+AVNCYP CP+P +ALG+PPH+D+S +T +LQSS+GL+IM
Sbjct: 192 ESLGLEKNYLQEEIEEGSQVMAVNCYPACPEPEMALGMPPHSDFSSLTILLQSSKGLQIM 251

Query: 250 DRADGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMD 309
           D  +  W  VP ++G+L V +GD VEV+SNG+YK V+HR  VN E  R+S +SLHSL + 
Sbjct: 252 D-CNKNWVCVPYIEGALIVQLGDQVEVMSNGIYKSVIHRVTVNKEVKRLSFASLHSLPLH 311

Query: 310 EKMKPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLK 351
           +K+ PA +LV+  N   Y E SFNDFL ++SSND  + + FIDT+K
Sbjct: 312 KKISPAPKLVNPNNAPAYGEFSFNDFLNYISSNDFIQ-ERFIDTIK 353

BLAST of CmaCh16G002270 vs. TAIR 10
Match: AT2G36690.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.2e-72
Identity = 139/346 (40.17%), Postives = 210/346 (60.69%), Query Frame = 0

Query: 19  EKGLNYVPNAYVIPTPQRPSLSPETAIV--------PIIDMASLRSNDSAERSLAIEALR 78
           E GL  VP  Y+ P P RP L+    ++        P+ID A L       R   +  + 
Sbjct: 26  ENGLTKVPTKYIWPEPDRPILTKSDKLIKPNKNLKLPLIDFAELL---GPNRPHVLRTIA 85

Query: 79  KACISLGFFQIINHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDG 138
           +AC + GFFQ++NHG+   V +  +   + FF LP +E+ KY S D+S  VRYGTS    
Sbjct: 86  EACKTYGFFQVVNHGMEGDVSKNMIDVCKRFFELPYEERSKYMSSDMSAPVRYGTSFNQI 145

Query: 139 VDKIKFWRVFLKHYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGL 198
            D +  WR FLK YAHPL D++  WP++P  +R     Y KE +++   + +AI ESL +
Sbjct: 146 KDNVFCWRDFLKLYAHPLPDYLPHWPSSPSDFRSSAATYAKETKEMFEMMVKAILESLEI 205

Query: 199 --RPYLSSKMAEGVQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSS-QGLEIMDRA 258
                 + ++ EG QVV VNCYPPCP+P + LG+PPH+DY  +T +LQ   +GL+I+ R 
Sbjct: 206 DGSDEAAKELEEGSQVVVVNCYPPCPEPELTLGMPPHSDYGFLTLLLQDEVEGLQILYR- 265

Query: 259 DGKWKMVPNVDGSLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKM 318
             +W  V  + GS  V+VGDH+E+ SNG YK V+HR +VNS + RIS++SLHS  +   +
Sbjct: 266 -DEWVTVDPIPGSFVVNVGDHLEIFSNGRYKSVLHRVLVNSTKPRISVASLHSFPLTSVV 325

Query: 319 KPAEELVDEQNPIKYKESSFNDFLKFLSSNDLAEGKSFIDTLKIQN 354
           KP+ +LVD+ NP +Y ++ F  FL++++S +  + K+F+++ KI +
Sbjct: 326 KPSPKLVDKHNPSQYMDTDFTTFLQYITSRE-PKWKNFLESRKIHH 365

BLAST of CmaCh16G002270 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.2e-61
Identity = 125/324 (38.58%), Postives = 201/324 (62.04%), Query Frame = 0

Query: 25  VPNAYVIPTPQRPSLSPETAI--VPIIDMASLRSNDSAERSLAIEALRKACISLGFFQII 84
           +P  YV P   RP LS  + +   P+ID++      S +RS  I+ + +AC   GFFQ+I
Sbjct: 15  LPENYVRPISDRPRLSEVSQLEDFPLIDLS------STDRSFLIQQIHQACARFGFFQVI 74

Query: 85  NHGISKTVMEEALSQAREFFNLPLKEKMKYKSDDVSKAVRYGTSLKDGVDKIKFWRVFLK 144
           NHG++K +++E +S AREFF++ ++EKMK  SDD +K  R  TS     +++  WR +L+
Sbjct: 75  NHGVNKQIIDEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSFNVKKEEVNNWRDYLR 134

Query: 145 HYAHPLEDWVDSWPTNPHTYREKMGQYTKEVRKVSLEIEEAITESLGL-RPYLSSKMAEG 204
            + +P+  +V+ WP+NP +++E + +Y++EVR+V  +IEE I+ESLGL + Y+   + E 
Sbjct: 135 LHCYPIHKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQ 194

Query: 205 VQVVAVNCYPPCPQPGVALGLPPHTDYSCITTILQSSQ--GLEIMDRADGKWKMVPNVDG 264
            Q +AVN YPPCP+P +  GLP HTD + +T +LQ +   GL+I+   DG+W  V     
Sbjct: 195 GQHMAVNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQIL--IDGQWFAVNPHPD 254

Query: 265 SLQVHVGDHVEVLSNGLYKGVVHRAIVNSERTRISISSLHSLGMDEKMKPAEEL----VD 324
           +  +++GD ++ LSNG+YK V HRA+ N+E  R+S++S         M PA+ L     D
Sbjct: 255 AFVINIGDQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDD 314

Query: 325 EQNPIKYKESSFNDFLKFLSSNDL 340
           E  P+ YK+ ++ ++ K   S +L
Sbjct: 315 ETKPV-YKDFTYAEYYKKFWSRNL 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q7XR842.5e-8347.83Flavanone 3-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-3 PE=1... [more]
Q7XR832.9e-7948.582-oxoglutarate-dependent dioxygenase 21, chloroplastic OS=Oryza sativa subsp. ja... [more]
Q6YYX96.9e-6539.18Probable 2-oxoglutarate-dependent dioxygenase SLC1 OS=Oryza sativa subsp. japoni... [more]
Q651B62.3e-6039.12Probable 2-oxoglutarate-dependent dioxygenase SLC2 OS=Oryza sativa subsp. japoni... [more]
Q9FLV08.7e-6038.58Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A6J1JAE64.3e-19592.65LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1E3T85.8e-19292.29LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A5A7TMH36.9e-17788.42Protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3BX866.9e-17788.42protein DOWNY MILDEW RESISTANCE 6 OS=Cucumis melo OX=3656 GN=LOC103494453 PE=3 S... [more]
A0A6J1CIV63.6e-16579.47flavanone 3-dioxygenase 3 OS=Momordica charantia OX=3673 GN=LOC111011393 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_022984408.18.9e-19592.65LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita maxima][more]
XP_022922584.11.2e-19192.29LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita moschata][more]
XP_023552054.12.5e-18991.03LOW QUALITY PROTEIN: flavanone 3-dioxygenase 3 [Cucurbita pepo subsp. pepo][more]
XP_008453850.11.4e-17688.42PREDICTED: protein DOWNY MILDEW RESISTANCE 6 [Cucumis melo] >KAA0044683.1 protei... [more]
XP_004146935.24.2e-17687.57flavanone 3-dioxygenase 3 [Cucumis sativus] >KGN53184.2 hypothetical protein Csa... [more]
Match NameE-valueIdentityDescription
AT5G07480.19.4e-11054.78KAR-UP oxidoreductase 1 [more]
AT3G60290.15.2e-9247.412-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G44800.16.8e-9248.272-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G36690.11.2e-7240.172-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G24530.16.2e-6138.582-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 205..297
e-value: 7.4E-21
score: 74.5
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 13..338
e-value: 1.0E-84
score: 286.6
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 46..156
e-value: 6.2E-25
score: 88.2
NoneNo IPR availablePANTHERPTHR47991:SF67KAR-UP OXIDOREDUCTASE 1coord: 10..338
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 10..338
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..336
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 198..301
score: 10.67921

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G002270.1CmaCh16G002270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding