CmaCh16G001860 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh16G001860
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	Cma_Chr16: 818987 .. 819571 (+)
RNA-Seq Expression	CmaCh16G001860
Synteny	CmaCh16G001860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

mRNA sequence

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Coding sequence (CDS)

ATGGGTCTTTCAATTTGCAAGAAAAGTTTGATTTTTGTGCAAATAATTCTCCAATTAACACTCATTCATTCAGCCATCACTCCACAAAGCAGCACACAGTTCATAAGAACTTCTTGCACTTCCACTAAATACCCTCGACTCTGTTTCTCATCTCTTTCAATCCACGCCAATTCCATCCAAACAAGCCCTCGCCTTTTGGCCACCACCGCCCTCTCCGTCACCCTCTCCTCCGTCAAGTCCGCCGCCACCCAAATCCTCAAGCTCTCCCACGCCCACGGCCTCCCTACAAGAGACGTCTCTGCTCTTAACGACTGTTTGGAAGAGCTCAGTGACTCTGTTGACTCCCTTGCCAGCTCCATTTCCGAGATGCCCAAGCTACGTGGCCATGACTTTGATCTCGTTATGAGCGATGTTCAGACATGGGTCAGCGCCGCCCTCACCGATGAGACCACTTGTAGTGAGGGGTTTCAAGGGAAGGCTGTCAAGGGCGGCGTTAAGGCGGCGGTTAGGAGGAGGATTGTGAACATTGCGCAATTGACCAGCAATGCTCTGTCTTTGATCAACCGGATTGCAGAGCTGCATTAG

Protein sequence

MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH

Homology

[Close]

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-37
Identity = 87/195 (44.62%), Postives = 132/195 (67.69%), Query Frame = 0


Query: 3   LSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTS 62
           + + K +L+F  ++    +I +A +   + QFI+TSCT T YP +C  SLS +A +IQ +
Sbjct: 1   MKLSKSTLVFSALL----VILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNN 60

Query: 63  PRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISE 122
           P+ LA+TAL V+L+  + A T + +L+   GL  R  +A++DCLEE+ DS+D ++ S  E
Sbjct: 61  PQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDE 120

Query: 123 MPKL---RGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQL 182
           M  L   +G+DF   MS+V+TWVSAALTDETTC +GF GK + G +K +VR ++V +A++
Sbjct: 121 MKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARV 180

Query: 183 TSNALSLINRIAELH 195
           TSNAL+L+N  A  H
Sbjct: 181 TSNALALVNNFAAKH 191

[Close]

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 79/167 (47.31%), Postives = 113/167 (67.66%), Query Frame = 0


Query: 32  TQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHA 91
           T+FI+ SC +T YP  CF SLS +A+ I+  PR LA TAL+V+++  KSA T + +++  
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 92  HGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLR----GHDFDLVMSDVQTWVSAALT 151
            G+  R   A+ DCLEE+ D+VD L++S+ E+  L     G DF   +S+V+TW SAALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 152 DETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           DET C +GF GKA+ G +K+ +R  IV++A+ TSNAL+LIN  A  H
Sbjct: 155 DETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 3.8e-33
Identity = 82/171 (47.95%), Postives = 118/171 (69.01%), Query Frame = 0


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           S  + Q++  +I+TSC  T Y  +C++SLS +A++I+++P+ LA  AL++TLSS KSA+ 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHD---FDLVMSDVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +   D   F++VMSDV+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF    VK  VK  VRR +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 81/173 (46.82%), Postives = 115/173 (66.47%), Query Frame = 0


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           +AIT + +  FI+ SC +T YP +C +SL+ +ANSIQTSPR LA TAL+VT++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMP---KLRGHD-FDLVMSDVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D F   MS+ Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           TW SAALT+  TCS+GF G+ + G VK +VR RI+N+ + TSNAL+LIN  A+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAK 200

[Close]

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.4e-30
Identity = 85/194 (43.81%), Postives = 126/194 (64.95%), Query Frame = 0


Query: 8   KSLIFVQIILQLTLIH-SAITP--QSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ-TSP 67
           K+ IF+ I+L +T++  S+ TP    S QFI +SC +T+YP LC  +LS +A  I+  + 
Sbjct: 4   KNTIFLVILLSITILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHNND 63

Query: 68  RLLATTALSVTLSSVKSAATQILKLS-HAHGLPTRDVSALNDCLEELSDSVDSLASSISE 127
           + LA TAL ++L+  KS    + KL+        R+  A+ DC+E L +SVD LA S+ E
Sbjct: 64  QDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKE 123

Query: 128 MPK----LRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQ 187
           + +    +   DF   MS+VQTWVSAALTDETTC +GF  +A+ G VK  +R ++V++AQ
Sbjct: 124 LARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQ 183

Query: 188 LTSNALSLINRIAE 193
           +TSNAL+L+N+ AE
Sbjct: 184 VTSNALALVNQFAE 197

[Close]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL
Match: A0A6J1JDH5 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 9.6e-96
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL
Match: A0A6J1E4J6 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.4e-94
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MGLSICKKSLIFVQIILQLT IHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL
Match: A0A0A0LB27 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 5.6e-80
Identity = 163/194 (84.02%), Postives = 181/194 (93.30%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK+LI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGISNSKKTLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLT
Sbjct: 121 SEMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+LH
Sbjct: 181 SNALSLINRIADLH 194

[Close]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL
Match: A0A0A0L8D1 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.1e-79
Identity = 162/193 (83.94%), Postives = 179/193 (92.75%), Query Frame = 0


Query: 2   GLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQT 61
           G+S  KKSLI  Q+ILQLTLIHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQT
Sbjct: 44  GISNSKKSLILFQLILQLTLIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQT 103

Query: 62  SPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSIS 121
           SPRLLAT ALSV+LSSVKS ATQILKLSH+HGLP+RDVSAL+DCLEELSDSVDSLA+SIS
Sbjct: 104 SPRLLATAALSVSLSSVKSTATQILKLSHSHGLPSRDVSALDDCLEELSDSVDSLAASIS 163

Query: 122 EMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTS 181
           EMPKLRG +FDL MS+VQTWVSAALTDETTCSEGFQGK V GGVK AVR +IVNIAQLTS
Sbjct: 164 EMPKLRGTNFDLAMSNVQTWVSAALTDETTCSEGFQGKTVNGGVKGAVRTKIVNIAQLTS 223

Query: 182 NALSLINRIAELH 195
           NALSLINRI +LH
Sbjct: 224 NALSLINRIGDLH 236

[Close]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL
Match: A0A6J1JNI8 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 7.6e-77
Identity = 162/202 (80.20%), Postives = 178/202 (88.12%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTL----IHSAITPQ----SSTQFIRTSCTSTKYPRLCFSSL 60
           MG SICK+SLI +  I QLTL    IHSAITPQ    SST+FI+TSC+ST YPRLCFSSL
Sbjct: 1   MGFSICKQSLILLVAIFQLTLNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSSL 60

Query: 61  SIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDS 120
           S+HA SIQTSPRLLAT ALSV++SSVKSA TQILKLSHAHGL  RDVSALNDCLEEL DS
Sbjct: 61  SLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120

Query: 121 VDSLASSISEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRR 180
           +DSL SSISEMPKLRG+DFD+VMS++QTWVSAALTDETTCSEGFQGK V GG+KAAVR +
Sbjct: 121 IDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRSK 180

Query: 181 IVNIAQLTSNALSLINRIAELH 195
           IVNIAQLTSNA+SLINRIAELH
Sbjct: 181 IVNIAQLTSNAISLINRIAELH 202

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: XP_022985514.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 359.4 bits (921), Expect = 2.0e-95
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: KAG6576795.1 (hypothetical protein SDJN03_24369, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 356.3 bits (913), Expect = 1.7e-94
Identity = 192/194 (98.97%), Postives = 193/194 (99.48%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILK+SHAHGLP RDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKISHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: XP_022922872.1 (21 kDa protein-like [Cucurbita moschata])

HSP 1 Score: 355.5 bits (911), Expect = 2.9e-94
Identity = 192/194 (98.97%), Postives = 192/194 (98.97%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MGLSICKKSLIFVQIILQLT IHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGLSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: XP_023552889.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 350.5 bits (898), Expect = 9.2e-93
Identity = 189/194 (97.42%), Postives = 191/194 (98.45%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+SICKKSLIFVQIILQLT IHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ
Sbjct: 1   MGVSICKKSLIFVQIILQLTFIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLP RDVSALNDCLEELSDSVDSLASSI
Sbjct: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDSLASSI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHD DLVMSDVQTWVSAALTDETTCSEGFQGKAV+GGVKAAVRRRIVNIAQLT
Sbjct: 121 SEMPKLRGHDIDLVMSDVQTWVSAALTDETTCSEGFQGKAVEGGVKAAVRRRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIAELH
Sbjct: 181 SNALSLINRIAELH 194

[Close]

BLAST of CmaCh16G001860 vs. NCBI nr
Match: XP_038882403.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 314.7 bits (805), Expect = 5.6e-82
Identity = 168/194 (86.60%), Postives = 182/194 (93.81%), Query Frame = 0


Query: 1   MGLSICKKSLIFVQIILQLTLIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQ 60
           MG+S  KK LI  Q+ILQLT IHSAITPQSST+FI++SC+ST YPRLCFSSLS+HAN+IQ
Sbjct: 1   MGVSNSKKILILFQLILQLTFIHSAITPQSSTEFIKSSCSSTTYPRLCFSSLSVHANAIQ 60

Query: 61  TSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSI 120
           TSPRLLAT ALSV+LSSVKSAATQILKLSHAHGLP+RDVSALNDCLEELSDSVDSLA+SI
Sbjct: 61  TSPRLLATAALSVSLSSVKSAATQILKLSHAHGLPSRDVSALNDCLEELSDSVDSLAASI 120

Query: 121 SEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLT 180
           SEMPKLRGHDFDLVMS+VQTWVSAALTDETTCSEGFQGK V GGVKA VR RIVNIAQLT
Sbjct: 121 SEMPKLRGHDFDLVMSNVQTWVSAALTDETTCSEGFQGKVVNGGVKATVRTRIVNIAQLT 180

Query: 181 SNALSLINRIAELH 195
           SNALSLINRIA+L+
Sbjct: 181 SNALSLINRIADLY 194

[Close]

BLAST of CmaCh16G001860 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 6.4e-44
Identity = 94/187 (50.27%), Postives = 137/187 (73.26%), Query Frame = 0


Query: 12  FVQIILQLTLIH----SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLA 71
           F+ II  L L+H    +  T  ++T+F+++SCT T YPRLCFSSLS HA+ IQTSP+L+A
Sbjct: 17  FLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMA 76

Query: 72  TTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLR 131
             AL++TL+S K  +  +++LS++  L  ++VSA+ DC+EEL D+++ L  SI EM +L 
Sbjct: 77  HAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLS 136

Query: 132 GHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLI 191
           G ++++ +SD+QTWVSAALTD  TC++GF+G+ + G VK  VR RI+ IA LTSNAL+LI
Sbjct: 137 GSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALI 196

Query: 192 NRIAELH 195
           N  A +H
Sbjct: 197 NHFASIH 202

[Close]

BLAST of CmaCh16G001860 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 4.9e-36
Identity = 88/195 (45.13%), Postives = 124/195 (63.59%), Query Frame = 0


Query: 10  LIFVQIILQLTLIHSAITPQS-----STQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPR 69
           L+ +  +L L L  +    Q+     +  FI++SC +T YP LC  SLS++AN IQTSP+
Sbjct: 9   LLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYANDIQTSPK 68

Query: 70  LLATTALSVTLSSVKSAATQILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMP 129
            LA TA++VTLS  +S    + +L+   GL  R+V A+ DC+EE++D+VD L  S+ E+ 
Sbjct: 69  RLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRLTKSVQEL- 128

Query: 130 KLRG-----HDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQL 189
           KL G       F   MS+ QTW SAALTDE TCS+GF G+ + G +K +VR RI+N+   
Sbjct: 129 KLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRARIMNVGHE 188

Query: 190 TSNALSLINRIAELH 195
           TSNALSLIN  A+ +
Sbjct: 189 TSNALSLINAFAKTY 202

[Close]

BLAST of CmaCh16G001860 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 1.9e-35
Identity = 79/167 (47.31%), Postives = 113/167 (67.66%), Query Frame = 0


Query: 32  TQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHA 91
           T+FI+ SC +T YP  CF SLS +A+ I+  PR LA TAL+V+++  KSA T + +++  
Sbjct: 35  TEFIKASCETTSYPDRCFQSLSSYASEIKKQPRKLAETALAVSIARAKSAKTYVSEMTDY 94

Query: 92  HGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLR----GHDFDLVMSDVQTWVSAALT 151
            G+  R   A+ DCLEE+ D+VD L++S+ E+  L     G DF   +S+V+TW SAALT
Sbjct: 95  KGITKRQHEAVADCLEEMGDTVDRLSNSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALT 154

Query: 152 DETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAELH 195
           DET C +GF GKA+ G +K+ +R  IV++A+ TSNAL+LIN  A  H
Sbjct: 155 DETACMDGFGGKAMAGELKSLIRTHIVSVAEETSNALALINDFASKH 201

[Close]

BLAST of CmaCh16G001860 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 142.9 bits (359), Expect = 2.7e-34
Identity = 82/171 (47.95%), Postives = 118/171 (69.01%), Query Frame = 0


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           S  + Q++  +I+TSC  T Y  +C++SLS +A++I+++P+ LA  AL++TLSS KSA+ 
Sbjct: 137 STYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASK 196

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMPKLRGHD---FDLVMSDVQT 143
            +  +SH  GL   +V A+ DC+EE+ DSV SL  SI E+  +   D   F++VMSDV+T
Sbjct: 197 FVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVET 256

Query: 144 WVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIA 192
           WVSAALT++ TC +GF    VK  VK  VRR +V +A+LTSNAL+LIN  A
Sbjct: 257 WVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of CmaCh16G001860 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 7.9e-34
Identity = 81/173 (46.82%), Postives = 115/173 (66.47%), Query Frame = 0


Query: 24  SAITPQSSTQFIRTSCTSTKYPRLCFSSLSIHANSIQTSPRLLATTALSVTLSSVKSAAT 83
           +AIT + +  FI+ SC +T YP +C +SL+ +ANSIQTSPR LA TAL+VT++  +S   
Sbjct: 28  AAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKV 87

Query: 84  QILKLSHAHGLPTRDVSALNDCLEELSDSVDSLASSISEMP---KLRGHD-FDLVMSDVQ 143
            + +L     L  R++ A+ DC+EE+ D+VD L  SI E+      +G D F   MS+ Q
Sbjct: 88  FVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQ 147

Query: 144 TWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVNIAQLTSNALSLINRIAE 193
           TW SAALT+  TCS+GF G+ + G VK +VR RI+N+ + TSNAL+LIN  A+
Sbjct: 148 TWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFAK 200

The following BLAST results are available for this feature:

BLAST of CmaCh16G001860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17407	3.3e-37	44.62	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SB37	2.6e-34	47.31	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	3.8e-33	47.95	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9STY5	1.1e-32	46.82	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SI72	1.4e-30	43.81	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]

BLAST of CmaCh16G001860 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDH5	9.6e-96	100.00	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483501 PE=4 SV=1	[more]
A0A6J1E4J6	1.4e-94	98.97	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1	[more]
A0A0A0LB27	5.6e-80	84.02	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G176245 PE=4 S...	[more]
A0A0A0L8D1	2.1e-79	83.94	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G175740 PE=4 S...	[more]
A0A6J1JNI8	7.6e-77	80.20	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1	[more]

BLAST of CmaCh16G001860 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985514.1	2.0e-95	100.00	21 kDa protein-like [Cucurbita maxima]	[more]
KAG6576795.1	1.7e-94	98.97	hypothetical protein SDJN03_24369, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022922872.1	2.9e-94	98.97	21 kDa protein-like [Cucurbita moschata]	[more]
XP_023552889.1	9.2e-93	97.42	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_038882403.1	5.6e-82	86.60	21 kDa protein-like [Benincasa hispida]	[more]

BLAST of CmaCh16G001860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	6.4e-44	50.27	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	4.9e-36	45.13	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	1.9e-35	47.31	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	2.7e-34	47.95	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	7.9e-34	46.82	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 30..186 e-value: 2.1E-48 score: 176.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 34..186 e-value: 1.1E-36 score: 126.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 7..189 e-value: 1.6E-36 score: 123.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 29..191 e-value: 1.4E-39 score: 137.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 24..191
None	No IPR available	PANTHER	PTHR31080:SF96	21 KDA PROTEIN-LIKE	coord: 24..191
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 37..191 e-value: 2.46484E-46 score: 147.97

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh16G001860.1	CmaCh16G001860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0004222	metalloendopeptidase activity