CmaCh16G000940 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G000940
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiondelta(1)-pyrroline-2-carboxylate reductase
LocationCma_Chr16: 417986 .. 421303 (-)
RNA-Seq ExpressionCmaCh16G000940
SyntenyCmaCh16G000940
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCCCATCCAATAACCCAAAAGACGAAACGAGTCTCACAAAGAAGATTACAGGCAGAGTTCCCATGTTTATCACCAAAGAGGATTTGGACTCCATTCTTCCACACCAAAATCTAATCGAGCACTTCCAATCATCTCTTTCTATCGTCTCCCCCACAATTTCCAGCCCAATGCGTCAAAGCCATACCGTTCAGCCCCATTCCTCTCTCCTCCTCATGCCATCTTGGTCTTCTTCTCCAGCTCTTCCCTACATAGGCGTCAAACTTGTAACCCACTTCCCCCAGAATTCCGCCTTAAACTTACCCGCAATTCATGCAAGTTACTCGCTCTTTAGCTCCACCACAGGCCAAACTCTGACCTCTATGGACGGCACTACCTTGACTCTTTACAGAACCTCTTGTATCTCTGGTTTAGCTGCGAAATATTTAGCCAGGCCTGATAGCAGGGTCATGGTTATGGTGGGTGCGGGTGCTTTGGGACCCCATTTGATCAAAGCACATTTGGCTGCGAGACCCAGTGTCAGGAAAGTGATTATTTGGAACAGAACGGAGGAAAGGGCAAAAATACTAGCCGATGAGATGCGGAAAAATGCAGGGATGGATGGACTCTGCTTCGAGAGTACTGAAAATCTGGATGATGCGATCTCGATGGCGGATATTGTGAGCTGTGCAACGAACTCAGAGGTTCCCCTGGTCAAAGGTAATTCTACTATTTACTTTTTTTTCTAAATTTAATTTGAAGTTAAAAAGAATCAATTAACTATCAAATGAGATCTTTTTAGTTATTGGGCCTATATGATTATCAAACAACCCTTAGTTCTTATAGTTTAAGGACTTCTAAAAAATGTGTTTGAAAAAATGGAATATTATATTAATATTTCAATGTCTTGGTTAACAGGTGATCAGTTGAAGGCTGGGGCGTACTTAAATTTGGTGGGGTCGTTTAAGCATTCAATGAGTGAGTGTGACGATTTAGCGATCAAGAGAGGAAGGGTTTTTGTGGACAGTGAGGCAGCGTTGGAGGAGGCAGGGGAGTTGGTGGGGGCTTTGGAAAGAGGGGTCATTGAGAAGAGGAGTATTTTGGGGAATCTGGTGGATTTGATCAAAGGGGAGGTTGTGGGAAGGAGGAATGAGGATGAGATTACTGTGTTCAAATCTGTTGGGTCTGCAGTGTTCGACCTTGTTGCAGCCCAGTTCGCGTACGAGAGAAGCATCTGAGCGCTCATAACAGGTGTTTGCTCTTTACCTCTTGCTTTTCTCCATCCGGATTTTTCTGTTTAATTTGCTTTAGATTCAATCCTTAATCCTTAATTTGCTTTAGACAAAGTCAAAAGCATTTGTCTTTTGGAATGGGCTGCTTTCCAAAGGACTCGTTCCATAATTTGCTTCTTAAAAAATAAAAATTCTAACTCGTTCGAGGAATTTTTTTTTTTTTTTTTTTTTTTTTTGGAGAGAAAAAGTTAGGATGATAGGTTAGAGAAATCAAGGGAGAATGGAAAGTTAAGAAGGAGATGTAGTAATGCATGTAATAGTGGGGAAAAAGGATTTGGTGGGTGAAAAGTTTGAGAGAAATGTTAACAGTAGCCAATGAAGTGTTAGAACTATAAAACAGACTAATTTGGGGGAGAATTAAATAAATATTAGGTAGAATATATAATTTTCAGAAAACACTAAACATAAATATATCAATATATCAATAAATAATATTTCTAGAAATGTATATTAATATTAAAATGTAAATACAATTAAGAATTTTTTTAATAAAGTAGTTTTAAAAAAATTTAAAATTAGATTCTATTTCCAATAATATGTTTAGAATATCATAAATGTCTTAATACTTCAAATTTGAATATTCTTTTTACTATTTATCTTCAAATAAAACAGAATTAAATTTGAATATTCTACTATTTATCTTCAAATAAAACAAAATAGTTTATTTATGTAAAAACATAATCTTTTAAAATCAAAAGAAATGGTTTATGGATAAATTATTAGTTTATTTATATAAAAACACAATCTTTTAAAATCAAAAGAAATGGTTTATGGAACCATCCATATTTTAACTAGAAAATAGGAAATAAGGTAGTTTTGTCATTTATGAATATTAGTTTTAAATCGACCTTGACTGAAAATGTTCATAAATTGATTGATTGAAGTCAGTCAACTCCGTTTTTCAGTCTTTAATGTTCACCCCTATTTATGAATTTGAAATAGAGACTAGATAATGCAACTGGTATAAAGTATTAAATAATAAATTAAAATTTTAAACCTAACATTGTTTATTTAGTTCGTAACTTAATGTAGTAAAAATCATATTTTATATTTTGATATTTATTAAAATTCATAAATTCACTAGGCAAAAAAATTGAAATGAAAGTTTTGTTCTAAGTTAAAAGTTTATAAATACAAAATTTAGTGTTTTAAAACTTATTAAAGATATAACAATTTTGAAAGTTCAAAAGTCAGCTAGACAAAATTCTTAAAGTTTAAGGAATAAATTAATAATTTAATCTACATTATAAGGAAAATTATTTATTTCACAACAGTTGATTAATTCAATTAAAGATATTATTTTTATGCCTAATCACTTAAATTAATGCTAACTCAAGTCAAGCTTAACTAGATGAAACATGTAATTAACCTACGTTAAAGGGTCATTTTCACTTCTAGGCTAGAAAATAGGCTAATGGCACAGCGTAAAACAGCAGCAAGAGAGATATTGGTGGAAAAGGCCAGTTGACAATAATCGAAGAATGCAATCACCCATACGTAATCAAATGAATCGACAATTTGAAATAAATTTACCTATTTCCATTTTGCTCTGAAACATTAAAGATTGGAACCTGAGAAACACAAACACACCCATAATTAGAGTGCAATGTGGTCGAGAAGGCTTACATTGTTCAGCATTCATTTTGGATGCGTTTGTTTCATTTTTGGAGGTTTGGAATGAATCCTCGGGAAAAGCAAGTCGTCCAGCTGTTTTTCCGCACTGAACTCTGAGGTCGCCTGCACAGACAAACTAGATGGTTAGGCTGGCAAATAACCCATTTCCTGACGAGAGAAATCGCTCCCGTTGGAAATATAAACTTTACACCTATGTAGCACAGAACCAAGAGGGGGAAAGCTCTCTATCGTTTTCTTTAGTCTGTTTGAAATTACTTTTAGGTGCTTAAAAAAAGTGTTGAAGAAAAAAAAAATCATTTTAAATTGGCTTTAAATTGTTTTTGCAAATCATTAATTAAAAAACATTACAACCACGTCAAACTATTTCACGACTGGAAAACAGACGAAAACATGGACAAAAGTTCATCA

mRNA sequence

ATGGCATCCCCATCCAATAACCCAAAAGACGAAACGAGTCTCACAAAGAAGATTACAGGCAGAGTTCCCATGTTTATCACCAAAGAGGATTTGGACTCCATTCTTCCACACCAAAATCTAATCGAGCACTTCCAATCATCTCTTTCTATCGTCTCCCCCACAATTTCCAGCCCAATGCGTCAAAGCCATACCGTTCAGCCCCATTCCTCTCTCCTCCTCATGCCATCTTGGTCTTCTTCTCCAGCTCTTCCCTACATAGGCGTCAAACTTGTAACCCACTTCCCCCAGAATTCCGCCTTAAACTTACCCGCAATTCATGCAAGTTACTCGCTCTTTAGCTCCACCACAGGCCAAACTCTGACCTCTATGGACGGCACTACCTTGACTCTTTACAGAACCTCTTGTATCTCTGGTTTAGCTGCGAAATATTTAGCCAGGCCTGATAGCAGGGTCATGGTTATGGTGGGTGCGGGTGCTTTGGGACCCCATTTGATCAAAGCACATTTGGCTGCGAGACCCAGTGTCAGGAAAGTGATTATTTGGAACAGAACGGAGGAAAGGGCAAAAATACTAGCCGATGAGATGCGGAAAAATGCAGGGATGGATGGACTCTGCTTCGAGAGTACTGAAAATCTGGATGATGCGATCTCGATGGCGGATATTGTGAGCTGTGCAACGAACTCAGAGGTTCCCCTGGTCAAAGGTGATCAGTTGAAGGCTGGGGCGTACTTAAATTTGGTGGGGTCGTTTAAGCATTCAATGAGTGAGTGTGACGATTTAGCGATCAAGAGAGGAAGGGTTTTTGTGGACAGTGAGGCAGCGTTGGAGGAGGCAGGGGAGTTGGTGGGGGCTTTGGAAAGAGGGGTCATTGAGAAGAGGAGTATTTTGGGGAATCTGGTGGATTTGATCAAAGGGGAGGTTGTGGGAAGGAGGAATGAGGATGAGATTACTGTGTTCAAATCTGTTGGGTCTGCAGTGTTCGACCTTGTTGCAGCCCAGTTCGCGTACGAGAGAAGCATCTGAGCGCTCATAACAGGTTTGGAATGAATCCTCGGGAAAAGCAAGTCGTCCAGCTGTTTTTCCGCACTGAACTCTGAGGTCGCCTGCACAGACAAACTAGATGGTTAGGCTGGCAAATAACCCATTTCCTGACGAGAGAAATCGCTCCCGTTGGAAATATAAACTTTACACCTATGTAGCACAGAACCAAGAGGGGGAAAGCTCTCTATCGTTTTCTTTAGTCTGTTTGAAATTACTTTTAGGTGCTTAAAAAAAGTGTTGAAGAAAAAAAAAATCATTTTAAATTGGCTTTAAATTGTTTTTGCAAATCATTAATTAAAAAACATTACAACCACGTCAAACTATTTCACGACTGGAAAACAGACGAAAACATGGACAAAAGTTCATCA

Coding sequence (CDS)

ATGGCATCCCCATCCAATAACCCAAAAGACGAAACGAGTCTCACAAAGAAGATTACAGGCAGAGTTCCCATGTTTATCACCAAAGAGGATTTGGACTCCATTCTTCCACACCAAAATCTAATCGAGCACTTCCAATCATCTCTTTCTATCGTCTCCCCCACAATTTCCAGCCCAATGCGTCAAAGCCATACCGTTCAGCCCCATTCCTCTCTCCTCCTCATGCCATCTTGGTCTTCTTCTCCAGCTCTTCCCTACATAGGCGTCAAACTTGTAACCCACTTCCCCCAGAATTCCGCCTTAAACTTACCCGCAATTCATGCAAGTTACTCGCTCTTTAGCTCCACCACAGGCCAAACTCTGACCTCTATGGACGGCACTACCTTGACTCTTTACAGAACCTCTTGTATCTCTGGTTTAGCTGCGAAATATTTAGCCAGGCCTGATAGCAGGGTCATGGTTATGGTGGGTGCGGGTGCTTTGGGACCCCATTTGATCAAAGCACATTTGGCTGCGAGACCCAGTGTCAGGAAAGTGATTATTTGGAACAGAACGGAGGAAAGGGCAAAAATACTAGCCGATGAGATGCGGAAAAATGCAGGGATGGATGGACTCTGCTTCGAGAGTACTGAAAATCTGGATGATGCGATCTCGATGGCGGATATTGTGAGCTGTGCAACGAACTCAGAGGTTCCCCTGGTCAAAGGTGATCAGTTGAAGGCTGGGGCGTACTTAAATTTGGTGGGGTCGTTTAAGCATTCAATGAGTGAGTGTGACGATTTAGCGATCAAGAGAGGAAGGGTTTTTGTGGACAGTGAGGCAGCGTTGGAGGAGGCAGGGGAGTTGGTGGGGGCTTTGGAAAGAGGGGTCATTGAGAAGAGGAGTATTTTGGGGAATCTGGTGGATTTGATCAAAGGGGAGGTTGTGGGAAGGAGGAATGAGGATGAGATTACTGTGTTCAAATCTGTTGGGTCTGCAGTGTTCGACCTTGTTGCAGCCCAGTTCGCGTACGAGAGAAGCATCTGA

Protein sequence

MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLVDLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Homology
BLAST of CmaCh16G000940 vs. ExPASy Swiss-Prot
Match: Q9FLY0 (Protein SAR DEFICIENT 4 OS=Arabidopsis thaliana OX=3702 GN=SARD4 PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.0e-105
Identity = 195/316 (61.71%), Postives = 248/316 (78.48%), Query Frame = 0

Query: 22  VPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQSHTVQPHSSLLLMPSWSSSP 81
           +P+FI  E   SIL H+ LI HF+++L   S TI+SP+RQ++TV   SSLLLMPSWSSS 
Sbjct: 4   LPVFIPAESFPSILSHETLINHFRTNLPKHSSTITSPVRQNYTVSSPSSLLLMPSWSSSS 63

Query: 82  ALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTLTLYRTSCISGLAA 141
           +LPY+GVKLVT+FP NS+ NLP IH SY+LFSSTTGQTL +MDGT LTLYRTS +SGL +
Sbjct: 64  SLPYMGVKLVTYFPHNSSQNLPGIHGSYTLFSSTTGQTLATMDGTVLTLYRTSSVSGLGS 123

Query: 142 KYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERAKILADEMRKNAGM 201
           K LAR DS+V++MVG+GAL PHLIK+HLAA+PS+R+VIIWNRT +RA+ LA+ + K+   
Sbjct: 124 KILARDDSQVLIMVGSGALAPHLIKSHLAAKPSLRRVIIWNRTPQRAQELAETLSKDPQH 183

Query: 202 DGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVGSFKHSMSECDDLA 261
             + F+S ++LD  I + DI+SCATNS VPLVKG+ LK G +L+LVGSF H M ECDD A
Sbjct: 184 KEISFDSHDSLDQIIPLGDIISCATNSTVPLVKGEFLKPGTHLDLVGSFSHEMKECDDNA 243

Query: 262 IKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLVDLIKGEVVGRRNEDEITVFKS 321
           I+RG VFVD++ A+ EAGEL GA ERGVI++  I GNLV+LIKG+  GR++  +ITVFKS
Sbjct: 244 IQRGSVFVDNDTAMIEAGELAGAFERGVIKREDICGNLVELIKGDKEGRKSSTDITVFKS 303

Query: 322 VGSAVFDLVAAQFAYE 338
           VGS   DL+ AQ  +E
Sbjct: 304 VGSGTVDLLTAQLVHE 319

BLAST of CmaCh16G000940 vs. ExPASy Swiss-Prot
Match: Q485R8 (Delta(1)-pyrroline-2-carboxylate reductase OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) OX=167879 GN=lhpI PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.9e-43
Identity = 111/314 (35.35%), Postives = 183/314 (58.28%), Query Frame = 0

Query: 37  HQNLIEHFQSSLSIV----SPTISSPMRQSHTVQP-----HSSLLLMPSWSSSPALPYIG 96
           HQNL  +F+  + ++    S   S P RQ +++ P     H +  L+PSW+       IG
Sbjct: 10  HQNL--NFEELIPLLKQSFSRPFSMPQRQVYSLAPEQSENHDAFALLPSWNEE----VIG 69

Query: 97  VKLVTHFPQNSAL-NLPAIHASYSLFSSTTGQTLTSMDGTTLTLYRTSCISGLAAKYLAR 156
            K  T+FP N+   +LP + +   LF   TG+ L  +DGT++T +RT+ IS LA++ L+R
Sbjct: 70  NKAFTYFPDNAKKHDLPGLFSKIMLFKRQTGEPLALVDGTSVTYWRTAAISALASQLLSR 129

Query: 157 PDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEER-AKILADEMRKNAGMDGLC 216
            +S+ +++ G G L  +L+KAHL  R  +++V +W R  ++ +K++AD    +     + 
Sbjct: 130 KNSQHLMLFGTGNLASYLVKAHLTVR-DIKQVTLWGRNAKKVSKLIAD---FSILYPAVT 189

Query: 217 FESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVGSFKHSMSECDDLAIKRG 276
           F+++ +++  ++ ADI+ CAT ++ PL  G+ + AG +++ +G+      ECD   I R 
Sbjct: 190 FKTSVDVNAEVASADIICCATGAKTPLFDGNSVSAGCHIDCLGNHMTDARECDTTTILRA 249

Query: 277 RVFVDS-EAALEEAGELVGALERGVIEKRSILGNLVDLIKGEVVGRRNEDEITVFKSVGS 336
           RVFVDS    L EAGEL+  +      K  I+G L D+ K   + R++ DEIT+FKSVG+
Sbjct: 250 RVFVDSLTNTLNEAGELLIPMAEDAFNKDEIVGELADMCKTPSMLRQSSDEITLFKSVGT 309

Query: 337 AVFDLVAAQFAYER 339
           A+ DLVAA    E+
Sbjct: 310 AISDLVAAHSVVEK 313

BLAST of CmaCh16G000940 vs. ExPASy Swiss-Prot
Match: Q9HDZ0 (Uncharacterized protein P11E10.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP11E10.01 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.1e-40
Identity = 112/322 (34.78%), Postives = 178/322 (55.28%), Query Frame = 0

Query: 25  FITKEDLDSILPHQNLIEHFQS--SLSIVSPT-ISSPMRQSH-TVQPHSSLLLMPSWSSS 84
           F+++E L S L    LI   +   + ++V PT +  P+ + + +   ++ LL+MP W   
Sbjct: 7   FLSRETLSSSLGWIPLINALREIFTENVVCPTRLHYPIDEDNPSSTANNILLIMPCWIPG 66

Query: 85  PALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTLTLYRTSCISGLA 144
               ++GVK V  FP+N+   LP++ + Y L  +TTG  L  +DG  LT  RT+  S LA
Sbjct: 67  ---KFLGVKQVNVFPENTKHGLPSLSSHYLLSDATTGCHLAQLDGNELTSRRTAAASALA 126

Query: 145 AKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERAKILADEMRKNAG 204
           + YL++ DS  ++++G+G +   LI AH + RP +R V IWN   E AK LA    K   
Sbjct: 127 SSYLSKEDSTSLLIIGSGKVAEKLIHAHCSVRP-IRSVRIWNHRFESAKSLASRASKELP 186

Query: 205 MDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVGSFKHSMSECDDL 264
              +     +NL++A+  +DI+S AT S+ P++ G  +  G +++LVG F   M E D  
Sbjct: 187 SIQIDAVPIQNLENAVKCSDIISSATLSKKPIIVGSWINPGTHIDLVGGFTPHMHEADSK 246

Query: 265 AIKRGRVFVDS-EAALEEAGELVGALERGVIEKRSILGNLVDLIKGEVVGRRN----EDE 324
            I+   VFVD+ + AL EAG+L+  ++ G+     ++ +L DL   +  GR       + 
Sbjct: 247 CIQISNVFVDTRKGALHEAGDLLTPIKEGLFSPNEVIADLFDLCNNKHSGRSQLKNPAEA 306

Query: 325 ITVFKSVGSAVFDLVAAQFAYE 338
           IT+FKSVG +  DL AA  AY+
Sbjct: 307 ITLFKSVGDSREDLAAASLAYK 324

BLAST of CmaCh16G000940 vs. ExPASy Swiss-Prot
Match: Q81HB0 (Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=BC_0906 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.7e-39
Identity = 101/274 (36.86%), Postives = 162/274 (59.12%), Query Frame = 0

Query: 69  SSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTL 128
           ++ L+MP  S +  L  +GVK+VT  PQN  +    I+    L     G+ L  ++G+ L
Sbjct: 49  NTALIMP--SVAEGLEALGVKIVTVVPQNKQIGKKTINGIVMLSDFQAGEPLALLEGSYL 108

Query: 129 TLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERA 188
           T+ RT  +SG+A KYLAR +++ + ++G G     + +A  A R  + KVI++NRTEE+A
Sbjct: 109 TMIRTGALSGVATKYLARHNAKTLCIIGTGEQAKGIAEAIFAVR-DIEKVILYNRTEEKA 168

Query: 189 KILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVG 248
              A  +++  G     ++     ++A+  ADI+   TN+  P V  + L+ G ++N VG
Sbjct: 169 YAFAQYIQEKFGKPAYVYKDP---NEAVREADIIVTTTNATTP-VFSEILQKGVHVNAVG 228

Query: 249 SFKHSMSECDDLAI-KRGRVFVDS-EAALEEAGELVGALERGVIEKRSILGNLVDLIKGE 308
           SF+ SM E    AI K  +V V+S EAAL+E G+L   ++ G+ +  +I   L  +I GE
Sbjct: 229 SFRPSMQELPSHAIAKANKVVVESKEAALDETGDLQVPIKEGLFKANAIHAELGQIISGE 288

Query: 309 VVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
             GR N++EIT+FKSVG AV D++ A++ YER++
Sbjct: 289 KAGRENDEEITIFKSVGLAVVDIIVAKYLYERAL 315

BLAST of CmaCh16G000940 vs. ExPASy Swiss-Prot
Match: Q6HMS8 (Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus thuringiensis subsp. konkukian (strain 97-27) OX=281309 GN=arcB PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 1.1e-38
Identity = 101/274 (36.86%), Postives = 163/274 (59.49%), Query Frame = 0

Query: 69  SSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTL 128
           ++ L+MP  S +  L  +G+K+VT  P+N  +    I+    L    TG+ L  ++G+ L
Sbjct: 49  NTALIMP--SVAEGLEALGLKVVTVVPENKKIGKKTINGIVMLSDFQTGEPLALLEGSYL 108

Query: 129 TLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERA 188
           T+ RT  +SG+A K+LAR +++ + ++G G     + +A  A R  + KVI++NRTEE+A
Sbjct: 109 TMIRTGALSGVATKHLARHNAKTLCIIGTGEQAKGIAEAVFAVR-DIEKVILYNRTEEKA 168

Query: 189 KILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVG 248
              +  +++  G          N ++AIS ADI+   TN+  P V  ++L+ G ++N VG
Sbjct: 169 YAFSQYIQEKFGKPAYVH---TNANEAISEADIIVTTTNASTP-VFSEKLQKGVHVNAVG 228

Query: 249 SFKHSMSECDDLAI-KRGRVFVDS-EAALEEAGELVGALERGVIEKRSILGNLVDLIKGE 308
           SFK SM E    AI    +V V+S EAAL+E G+L   ++ G+ +  +I   L  +I GE
Sbjct: 229 SFKPSMQELPSHAIVGANKVVVESKEAALDETGDLQVPIKEGLFKANAIHAELGQIISGE 288

Query: 309 VVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
             GR N++EITVFKSVG AV D++ A++ YE+++
Sbjct: 289 KAGRENDEEITVFKSVGLAVVDIIVAKYLYEKAV 315

BLAST of CmaCh16G000940 vs. ExPASy TrEMBL
Match: A0A6J1J2T7 (protein SAR DEFICIENT 4-like OS=Cucurbita maxima OX=3661 GN=LOC111482883 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.9e-184
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII
Sbjct: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA
Sbjct: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of CmaCh16G000940 vs. ExPASy TrEMBL
Match: A0A6J1E7X4 (protein SAR DEFICIENT 4 OS=Cucurbita moschata OX=3662 GN=LOC111430702 PE=4 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 1.8e-177
Identity = 326/340 (95.88%), Postives = 332/340 (97.65%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASP NNPKD+TSLTKKITGRVPMFITKEDLDSILPHQ LIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPPNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQYLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHT+QPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTIQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TS+DGTTLTLYRTSCISGLA KYLARPDSRVMVMVGAGALGPHLIKAHLA RPSVRKVII
Sbjct: 121 TSIDGTTLTLYRTSCISGLATKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAK+LADEMRKNAGMDGLCFEST NLDDAIS+ADIVSCATNSEVPLVKGD+LKA
Sbjct: 181 WNRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSM ECDDLAIKRGRVFVDSE ALEEAGELVGA ERGVIEKRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGAFERGVIEKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of CmaCh16G000940 vs. ExPASy TrEMBL
Match: A0A5A7SWS6 (Delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001900 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.0e-158
Identity = 298/342 (87.13%), Postives = 316/342 (92.40%), Query Frame = 0

Query: 1   MASPSNNPKDET--SLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSP 60
           MAS SNN KD+T  +LTKKIT R PM ITKE LDSIL HQNLIEHFQSSL IV PTISSP
Sbjct: 1   MASTSNNLKDDTKSNLTKKITVRTPMVITKEALDSILQHQNLIEHFQSSLPIVFPTISSP 60

Query: 61  MRQSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQ 120
           MRQSH VQPHSSLLLMPSWSSSP+LPYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQ
Sbjct: 61  MRQSHVVQPHSSLLLMPSWSSSPSLPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQ 120

Query: 121 TLTSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKV 180
           TL SMDGT LTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLA RPSV+KV
Sbjct: 121 TLGSMDGTALTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVKKV 180

Query: 181 IIWNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQL 240
           IIWNRTEERAK LADEMR+NAG+DG+CFES+ NLDDAIS+ADIVSCATNSEVPLVKGD+L
Sbjct: 181 IIWNRTEERAKRLADEMRENAGLDGVCFESSANLDDAISVADIVSCATNSEVPLVKGDKL 240

Query: 241 KAGAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGN 300
           K GAYLNLVGSFK SM ECDD+AIKRGRVFVD E ALEEAGEL+GALERGVIEK  ILG 
Sbjct: 241 KCGAYLNLVGSFKPSMRECDDMAIKRGRVFVDCEEALEEAGELLGALERGVIEKSDILG- 300

Query: 301 LVDLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           LVDLIKG++VGRRNEDEI+VFKSVGSA+FDLVAAQFAYE +I
Sbjct: 301 LVDLIKGQIVGRRNEDEISVFKSVGSAMFDLVAAQFAYETTI 341

BLAST of CmaCh16G000940 vs. ExPASy TrEMBL
Match: A0A1S3AZ51 (delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC103484130 PE=4 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 9.0e-158
Identity = 298/342 (87.13%), Postives = 316/342 (92.40%), Query Frame = 0

Query: 1   MASPSNNPKDET--SLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSP 60
           MAS SNN KD+T  +LTKKIT R PM ITKE LDSIL HQNLIEHFQSSL IV PTISSP
Sbjct: 66  MASTSNNLKDDTKSNLTKKITVRTPMVITKEALDSILQHQNLIEHFQSSLPIVFPTISSP 125

Query: 61  MRQSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQ 120
           MRQSH VQPHSSLLLMPSWSSSP+LPYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQ
Sbjct: 126 MRQSHVVQPHSSLLLMPSWSSSPSLPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQ 185

Query: 121 TLTSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKV 180
           TL SMDGT LTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLA RPSV+KV
Sbjct: 186 TLGSMDGTALTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVKKV 245

Query: 181 IIWNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQL 240
           IIWNRTEERAK LADEMR+NAG+DG+CFES+ NLDDAIS+ADIVSCATNSEVPLVKGD+L
Sbjct: 246 IIWNRTEERAKRLADEMRENAGLDGVCFESSANLDDAISVADIVSCATNSEVPLVKGDKL 305

Query: 241 KAGAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGN 300
           K GAYLNLVGSFK SM ECDD+AIKRGRVFVD E ALEEAGEL+GALERGVIEK  ILG 
Sbjct: 306 KCGAYLNLVGSFKPSMRECDDMAIKRGRVFVDCEEALEEAGELLGALERGVIEKSDILG- 365

Query: 301 LVDLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           LVDLIKG++VGRRNEDEI+VFKSVGSA+FDLVAAQFAYE +I
Sbjct: 366 LVDLIKGQIVGRRNEDEISVFKSVGSAMFDLVAAQFAYETTI 406

BLAST of CmaCh16G000940 vs. ExPASy TrEMBL
Match: A0A5D3DHZ1 (Delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001180 PE=4 SV=1)

HSP 1 Score: 565.5 bits (1456), Expect = 1.5e-157
Identity = 298/342 (87.13%), Postives = 315/342 (92.11%), Query Frame = 0

Query: 1   MASPSNNPKDET--SLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSP 60
           MAS SNN KD+T  +LTKKIT R PM ITKE LDSIL HQNLIEHFQSSL IV PTISSP
Sbjct: 1   MASTSNNLKDDTKSNLTKKITVRTPMVITKEALDSILQHQNLIEHFQSSLPIVFPTISSP 60

Query: 61  MRQSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQ 120
           MRQSH VQPHSSLLLMPSWSSSP+LPYIGVKLVTHFPQNSA+NLPAIHASYSLFSSTTGQ
Sbjct: 61  MRQSHVVQPHSSLLLMPSWSSSPSLPYIGVKLVTHFPQNSAINLPAIHASYSLFSSTTGQ 120

Query: 121 TLTSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKV 180
           TL SMDGT LTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLA RPSV+KV
Sbjct: 121 TLGSMDGTALTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVKKV 180

Query: 181 IIWNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQL 240
           IIWNRTEERAK LADEMR+NAG DG+CFES+ NLDDAIS+ADIVSCATNSEVPLVKGD+L
Sbjct: 181 IIWNRTEERAKRLADEMRENAGFDGVCFESSANLDDAISVADIVSCATNSEVPLVKGDKL 240

Query: 241 KAGAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGN 300
           K GAYLNLVGSFK SM ECDD+AIKRGRVFVD E ALEEAGEL+GALERGVIEK  ILG 
Sbjct: 241 KCGAYLNLVGSFKPSMRECDDMAIKRGRVFVDCEEALEEAGELLGALERGVIEKSDILG- 300

Query: 301 LVDLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           LVDLIKG++VGRRNEDEI+VFKSVGSA+FDLVAAQFAYE +I
Sbjct: 301 LVDLIKGQIVGRRNEDEISVFKSVGSAMFDLVAAQFAYETTI 341

BLAST of CmaCh16G000940 vs. NCBI nr
Match: XP_022984671.1 (protein SAR DEFICIENT 4-like [Cucurbita maxima] >XP_022984672.1 protein SAR DEFICIENT 4-like [Cucurbita maxima])

HSP 1 Score: 654.8 bits (1688), Expect = 4.0e-184
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII
Sbjct: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA
Sbjct: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of CmaCh16G000940 vs. NCBI nr
Match: XP_023552312.1 (protein SAR DEFICIENT 4-like [Cucurbita pepo subsp. pepo] >XP_023552313.1 protein SAR DEFICIENT 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 643.3 bits (1658), Expect = 1.2e-180
Identity = 333/340 (97.94%), Postives = 337/340 (99.12%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASPSNNPKD+TSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPSNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII
Sbjct: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAK+LADEMRKNAGMDGLCFEST NLDDAIS+ADIVSCATNSEVPLVKGD+LKA
Sbjct: 181 WNRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSM ECDDLAIKRGRVFVDSE ALEEAGELVGALERGVIEKRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGALERGVIEKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of CmaCh16G000940 vs. NCBI nr
Match: XP_022922838.1 (protein SAR DEFICIENT 4 [Cucurbita moschata] >XP_022922839.1 protein SAR DEFICIENT 4 [Cucurbita moschata] >KAG7014743.1 lhpI, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 631.7 bits (1628), Expect = 3.6e-177
Identity = 326/340 (95.88%), Postives = 332/340 (97.65%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASP NNPKD+TSLTKKITGRVPMFITKEDLDSILPHQ LIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPPNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQYLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHT+QPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTIQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TS+DGTTLTLYRTSCISGLA KYLARPDSRVMVMVGAGALGPHLIKAHLA RPSVRKVII
Sbjct: 121 TSIDGTTLTLYRTSCISGLATKYLARPDSRVMVMVGAGALGPHLIKAHLAVRPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAK+LADEMRKNAGMDGLCFEST NLDDAIS+ADIVSCATNSEVPLVKGD+LKA
Sbjct: 181 WNRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSM ECDDLAIKRGRVFVDSE ALEEAGELVGA ERGVIEKRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGAFERGVIEKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 340

BLAST of CmaCh16G000940 vs. NCBI nr
Match: KAG6576692.1 (Protein SAR DEFICIENT 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 601.7 bits (1550), Expect = 4.0e-168
Identity = 310/323 (95.98%), Postives = 316/323 (97.83%), Query Frame = 0

Query: 1   MASPSNNPKDETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMR 60
           MASP NNPKD+TSLTKKITGRVPMFITKEDLDSILPHQ LIEHFQSSLSIVSPTISSPMR
Sbjct: 1   MASPPNNPKDKTSLTKKITGRVPMFITKEDLDSILPHQYLIEHFQSSLSIVSPTISSPMR 60

Query: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120
           QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL
Sbjct: 61  QSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTL 120

Query: 121 TSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180
           TS+DGTTLTLYRTSCISGLA KYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII
Sbjct: 121 TSIDGTTLTLYRTSCISGLATKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVII 180

Query: 181 WNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKA 240
           WNRTEERAK+LADEMRKNAGMDGLCFEST NLDDAIS+ADIVSCATNSEVPLVKGD+LKA
Sbjct: 181 WNRTEERAKVLADEMRKNAGMDGLCFESTGNLDDAISVADIVSCATNSEVPLVKGDKLKA 240

Query: 241 GAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLV 300
           GAYLNLVGSFKHSM ECDDLAIKRGRVFVDSE ALEEAGELVGA ERGVI+KRSILGNLV
Sbjct: 241 GAYLNLVGSFKHSMRECDDLAIKRGRVFVDSEEALEEAGELVGAFERGVIDKRSILGNLV 300

Query: 301 DLIKGEVVGRRNEDEITVFKSVG 324
           DLIKGEVVGRRNEDEITVFKSVG
Sbjct: 301 DLIKGEVVGRRNEDEITVFKSVG 323

BLAST of CmaCh16G000940 vs. NCBI nr
Match: XP_038880486.1 (protein SAR DEFICIENT 4 [Benincasa hispida] >XP_038880489.1 protein SAR DEFICIENT 4 [Benincasa hispida])

HSP 1 Score: 575.9 bits (1483), Expect = 2.4e-160
Identity = 303/342 (88.60%), Postives = 314/342 (91.81%), Query Frame = 0

Query: 1   MASPSNNPKD--ETSLTKKITGRVPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSP 60
           MAS S+NPKD  ET+L KKIT R PMFITKE LDSIL HQ L EHFQSSL IV  TI SP
Sbjct: 1   MASTSSNPKDETETNLAKKITARSPMFITKEALDSILTHQRLSEHFQSSLPIVFHTIFSP 60

Query: 61  MRQSHTVQPHSSLLLMPSWSSSPALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQ 120
           MRQSH VQPHSSLLLMPSWSSSP+LPYIGVKLV HFPQNSA+NLPAIHASYSLFSSTTGQ
Sbjct: 61  MRQSHAVQPHSSLLLMPSWSSSPSLPYIGVKLVIHFPQNSAINLPAIHASYSLFSSTTGQ 120

Query: 121 TLTSMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKV 180
           TL SMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSV KV
Sbjct: 121 TLASMDGTTLTLYRTSCISGLAAKYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVNKV 180

Query: 181 IIWNRTEERAKILADEMRKNAGMDGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQL 240
           IIWNRTEERA+ LADEMRKNAG+DG+CFES+ NLDDAI  ADIVSCATNSEVPLVKGD+L
Sbjct: 181 IIWNRTEERAQKLADEMRKNAGLDGVCFESSANLDDAIPEADIVSCATNSEVPLVKGDKL 240

Query: 241 KAGAYLNLVGSFKHSMSECDDLAIKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGN 300
           KAGAYLNLVGSFKHSM ECDD+AIKRGRVFVD E ALEEAGELVGALERGVIEKR ILGN
Sbjct: 241 KAGAYLNLVGSFKHSMRECDDMAIKRGRVFVDGEEALEEAGELVGALERGVIEKRDILGN 300

Query: 301 LVDLIKGEVVGRRNEDEITVFKSVGSAVFDLVAAQFAYERSI 341
           LVDLIKGEVVGRRN DEITVFKSVGSAVFDLVAAQFAYE +I
Sbjct: 301 LVDLIKGEVVGRRNGDEITVFKSVGSAVFDLVAAQFAYETTI 342

BLAST of CmaCh16G000940 vs. TAIR 10
Match: AT5G52810.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 384.8 bits (987), Expect = 7.2e-107
Identity = 195/316 (61.71%), Postives = 248/316 (78.48%), Query Frame = 0

Query: 22  VPMFITKEDLDSILPHQNLIEHFQSSLSIVSPTISSPMRQSHTVQPHSSLLLMPSWSSSP 81
           +P+FI  E   SIL H+ LI HF+++L   S TI+SP+RQ++TV   SSLLLMPSWSSS 
Sbjct: 4   LPVFIPAESFPSILSHETLINHFRTNLPKHSSTITSPVRQNYTVSSPSSLLLMPSWSSSS 63

Query: 82  ALPYIGVKLVTHFPQNSALNLPAIHASYSLFSSTTGQTLTSMDGTTLTLYRTSCISGLAA 141
           +LPY+GVKLVT+FP NS+ NLP IH SY+LFSSTTGQTL +MDGT LTLYRTS +SGL +
Sbjct: 64  SLPYMGVKLVTYFPHNSSQNLPGIHGSYTLFSSTTGQTLATMDGTVLTLYRTSSVSGLGS 123

Query: 142 KYLARPDSRVMVMVGAGALGPHLIKAHLAARPSVRKVIIWNRTEERAKILADEMRKNAGM 201
           K LAR DS+V++MVG+GAL PHLIK+HLAA+PS+R+VIIWNRT +RA+ LA+ + K+   
Sbjct: 124 KILARDDSQVLIMVGSGALAPHLIKSHLAAKPSLRRVIIWNRTPQRAQELAETLSKDPQH 183

Query: 202 DGLCFESTENLDDAISMADIVSCATNSEVPLVKGDQLKAGAYLNLVGSFKHSMSECDDLA 261
             + F+S ++LD  I + DI+SCATNS VPLVKG+ LK G +L+LVGSF H M ECDD A
Sbjct: 184 KEISFDSHDSLDQIIPLGDIISCATNSTVPLVKGEFLKPGTHLDLVGSFSHEMKECDDNA 243

Query: 262 IKRGRVFVDSEAALEEAGELVGALERGVIEKRSILGNLVDLIKGEVVGRRNEDEITVFKS 321
           I+RG VFVD++ A+ EAGEL GA ERGVI++  I GNLV+LIKG+  GR++  +ITVFKS
Sbjct: 244 IQRGSVFVDNDTAMIEAGELAGAFERGVIKREDICGNLVELIKGDKEGRKSSTDITVFKS 303

Query: 322 VGSAVFDLVAAQFAYE 338
           VGS   DL+ AQ  +E
Sbjct: 304 VGSGTVDLLTAQLVHE 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLY01.0e-10561.71Protein SAR DEFICIENT 4 OS=Arabidopsis thaliana OX=3702 GN=SARD4 PE=1 SV=1[more]
Q485R81.9e-4335.35Delta(1)-pyrroline-2-carboxylate reductase OS=Colwellia psychrerythraea (strain ... [more]
Q9HDZ01.1e-4034.78Uncharacterized protein P11E10.01 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Q81HB03.7e-3936.86Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus cereus (strain ATCC 14579... [more]
Q6HMS81.1e-3836.86Delta(1)-pyrroline-2-carboxylate reductase OS=Bacillus thuringiensis subsp. konk... [more]
Match NameE-valueIdentityDescription
A0A6J1J2T71.9e-184100.00protein SAR DEFICIENT 4-like OS=Cucurbita maxima OX=3661 GN=LOC111482883 PE=4 SV... [more]
A0A6J1E7X41.8e-17795.88protein SAR DEFICIENT 4 OS=Cucurbita moschata OX=3662 GN=LOC111430702 PE=4 SV=1[more]
A0A5A7SWS69.0e-15887.13Delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3AZ519.0e-15887.13delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo OX=3656 GN=LOC1034841... [more]
A0A5D3DHZ11.5e-15787.13Delta(1)-pyrroline-2-carboxylate reductase OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
XP_022984671.14.0e-184100.00protein SAR DEFICIENT 4-like [Cucurbita maxima] >XP_022984672.1 protein SAR DEFI... [more]
XP_023552312.11.2e-18097.94protein SAR DEFICIENT 4-like [Cucurbita pepo subsp. pepo] >XP_023552313.1 protei... [more]
XP_022922838.13.6e-17795.88protein SAR DEFICIENT 4 [Cucurbita moschata] >XP_022922839.1 protein SAR DEFICIE... [more]
KAG6576692.14.0e-16895.98Protein SAR DEFICIENT 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038880486.12.4e-16088.60protein SAR DEFICIENT 4 [Benincasa hispida] >XP_038880489.1 protein SAR DEFICIEN... [more]
Match NameE-valueIdentityDescription
AT5G52810.17.2e-10761.71NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 146..323
e-value: 3.3E-80
score: 271.3
NoneNo IPR availablePANTHERPTHR13812:SF19KETIMINE REDUCTASE MU-CRYSTALLINcoord: 24..337
IPR023401Ornithine cyclodeaminase, N-terminalGENE3D3.30.1780.10ornithine cyclodeaminase, domain 1coord: 53..336
e-value: 3.3E-80
score: 271.3
IPR003462Ornithine cyclodeaminase/mu-crystallinPIRSFPIRSF001439CryMcoord: 17..340
e-value: 1.1E-69
score: 232.9
IPR003462Ornithine cyclodeaminase/mu-crystallinPFAMPF02423OCD_Mu_crystallcoord: 23..338
e-value: 5.1E-61
score: 206.3
IPR003462Ornithine cyclodeaminase/mu-crystallinPANTHERPTHR13812KETIMINE REDUCTASE MU-CRYSTALLINcoord: 24..337
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 23..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G000940.1CmaCh16G000940.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0062034 L-pipecolic acid biosynthetic process
biological_process GO:1901672 positive regulation of systemic acquired resistance
cellular_component GO:0009507 chloroplast
molecular_function GO:0062046 dehydropipecolic acid reductase