CmaCh16G000670 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh16G000670
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionTrehalose 6-phosphate phosphatase
LocationCma_Chr16: 287937 .. 289940 (+)
RNA-Seq ExpressionCmaCh16G000670
SyntenyCmaCh16G000670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACTTCTCTCTTCTTTTTAGCCATGTTTTGCTGAAAGGAAAATCCGTGCTCCTAATTTTCCTTCCCTTCACTTTCGGTCTTCCCGCCCTCCGCCGCACACTGCTCTGGAGATCGATAATGGGACTCTCCGGGCTGCTTTCCGGTCACCGGAACTCCGTACAAGGTTCCGATTTCCACAACTACACTTCATGGCTGGTACGAAATTAAATTTTAAATCATCTTTTTCTTTTGAATTTCTGGGTTATTAAATGTTGATTTCTGTGTATTAGAAACAATACCCCAGTGGTTTGGAATCGTTTGAGTCCATAATGAAAGAGCTACCGAGGAAGAAGATTGTGGTGTTCTTGGATTATGATGGAACCCTTTCTCCCATTGTGGATGATCCTGATCGAGCTTTCATGTCTCCTGAGGTAAATTATTACTGTTATAAATTTTACATATTCGATTCAAATATAAACGTTCCACACTTTAATATACATTCAAATCTTAGAAAGATTGCTAATGCCTAAATGCTTGATTTTGTTTTGGAAGCAGTTGAGATATGTTATATCCATAATATCCTTTTTTTCTTTTTTCTTTTTTTTTTTTTGCTTAGTTGTTTGACCTTATTTAGAAATTATAAAATGATTAGTTTTTTTTCTTTGTTCTTTGAATTTCTACACTAAAAATATGGGTTTTATTATTAATAATTTTTAAAGGTTGACCTATATCTTTTTGGATTTGATTCTTTTAATTTAGTCTCTTTATTGTTTAATATATATATTTTTCTGTGTGTATAAATGCAGATGAGAGAAGCTGTGAGAGAAGTGGCAAAAGGCTTTCCGACTGCCATAATAAGCGGAAGAAGCCGAGAAAAGGTAATTATTTAAATTTTAATTGTTTATATATATATATTAAAGAAATTAATTGAAAATTTCAGGTAAAGGAGTTCGTGAAATTGAGGAATCTGCACTATGCTGGGAGCCATGGATTAGACATTATGTCTCCAAATTCGGACAATGAGGTTCTTCTAGTTTTATAATTATAATATTTCCGACTTATATCATTTATCAACGTTAACATAACATTATACGTTTGATTGTAGGCTAATGGGGTTTCCTATCAACCAGCCAAGAAGTTTTTACCAAAAATTCAACAAGTAATTTCTTGTCGGAAAAATCGAATTATTATCATTATTATTTTTGTTTTTTATGATACGAGGTTTGAAATATGGAAAACATTTAAAACGTGACAGATAAAACGCGTATTAGAGGAAGAAACTAGAAAGATCGAAGGTGCATTGGTGGAGGATAATACGTTTTGTGTTTCTGTACACTTTCGGCACGTGCACGAAAGGGTAAAGATCTCGATCTTTGTCGATTATGTATGTGTATGAACGTGATATGGATAAAGATTAAGGAAACGAATCAATGTGTTATTGACTTATGGCAGGAGTTGGCGGAATTAGAAAAGAAGGTAGAGACTGTTATGGAAAAATATCCTGATTTTCATGTAACGCAGGGCAAAAAGGTGCATAAATCCTGAAAATAAGCATGATTTTGTTTGTTGAAAATGAAGCCATATTCTTTGCTAATATGGTATGAACATTTGAAGGTTATGGAAATAAGGCCGACCATTAATTGGAACAAAGGGCATGCAATGGAGTATTATCTGCACAGTCTGGGACATAACGACACCGACGATGTGGTTCCTTTGTACATTGGCGATGATCAAACGGACGAGGACGCTTTCAAGGTAAGCAAGCATATGCAAAAGCTGGATGTTGTTAATACTCATGAATATTGAAATGGGTATTAAACTGAAGTGGGATGAGTATGAGTTTTGTAGGTAATACAAAGGAAAGGACATGGAATTGCGATACTCGTGGCATCCATTCCAAAGCACACAAACGCTTCCTACTCGTTGAAGGACCCATCGGAGGTTCTTGCTTTTTTGCTAAGGCTGGCCAGATGGAGGAACTCCACAGCCCCTTCAATTACAACCCCATCTCCTAATATTTAA

mRNA sequence

ATGAACTTCTCTCTTCTTTTTAGCCATGTTTTGCTGAAAGGAAAATCCGTGCTCCTAATTTTCCTTCCCTTCACTTTCGGTCTTCCCGCCCTCCGCCGCACACTGCTCTGGAGATCGATAATGGGACTCTCCGGGCTGCTTTCCGGTCACCGGAACTCCGTACAAGGTTCCGATTTCCACAACTACACTTCATGGCTGAAACAATACCCCAGTGGTTTGGAATCGTTTGAGTCCATAATGAAAGAGCTACCGAGGAAGAAGATTGTGGTGTTCTTGGATTATGATGGAACCCTTTCTCCCATTGTGGATGATCCTGATCGAGCTTTCATGTCTCCTGAGATGAGAGAAGCTGTGAGAGAAGTGGCAAAAGGCTTTCCGACTGCCATAATAAGCGGAAGAAGCCGAGAAAAGGTAAAGGAGTTCGTGAAATTGAGGAATCTGCACTATGCTGGGAGCCATGGATTAGACATTATGTCTCCAAATTCGGACAATGAGGCTAATGGGGTTTCCTATCAACCAGCCAAGAAGTTTTTACCAAAAATTCAACAAATAAAACGCGTATTAGAGGAAGAAACTAGAAAGATCGAAGGTGCATTGGTGGAGGATAATACGTTTTGTGTTTCTGTACACTTTCGGCACGTGCACGAAAGGGAGTTGGCGGAATTAGAAAAGAAGGTAGAGACTGTTATGGAAAAATATCCTGATTTTCATGTAACGCAGGGCAAAAAGGTTATGGAAATAAGGCCGACCATTAATTGGAACAAAGGGCATGCAATGGAGTATTATCTGCACAGTCTGGGACATAACGACACCGACGATGTGGTTCCTTTGTACATTGGCGATGATCAAACGGACGAGGACGCTTTCAAGGTAATACAAAGGAAAGGACATGGAATTGCGATACTCGTGGCATCCATTCCAAAGCACACAAACGCTTCCTACTCGTTGAAGGACCCATCGGAGGTTCTTGCTTTTTTGCTAAGGCTGGCCAGATGGAGGAACTCCACAGCCCCTTCAATTACAACCCCATCTCCTAATATTTAA

Coding sequence (CDS)

ATGAACTTCTCTCTTCTTTTTAGCCATGTTTTGCTGAAAGGAAAATCCGTGCTCCTAATTTTCCTTCCCTTCACTTTCGGTCTTCCCGCCCTCCGCCGCACACTGCTCTGGAGATCGATAATGGGACTCTCCGGGCTGCTTTCCGGTCACCGGAACTCCGTACAAGGTTCCGATTTCCACAACTACACTTCATGGCTGAAACAATACCCCAGTGGTTTGGAATCGTTTGAGTCCATAATGAAAGAGCTACCGAGGAAGAAGATTGTGGTGTTCTTGGATTATGATGGAACCCTTTCTCCCATTGTGGATGATCCTGATCGAGCTTTCATGTCTCCTGAGATGAGAGAAGCTGTGAGAGAAGTGGCAAAAGGCTTTCCGACTGCCATAATAAGCGGAAGAAGCCGAGAAAAGGTAAAGGAGTTCGTGAAATTGAGGAATCTGCACTATGCTGGGAGCCATGGATTAGACATTATGTCTCCAAATTCGGACAATGAGGCTAATGGGGTTTCCTATCAACCAGCCAAGAAGTTTTTACCAAAAATTCAACAAATAAAACGCGTATTAGAGGAAGAAACTAGAAAGATCGAAGGTGCATTGGTGGAGGATAATACGTTTTGTGTTTCTGTACACTTTCGGCACGTGCACGAAAGGGAGTTGGCGGAATTAGAAAAGAAGGTAGAGACTGTTATGGAAAAATATCCTGATTTTCATGTAACGCAGGGCAAAAAGGTTATGGAAATAAGGCCGACCATTAATTGGAACAAAGGGCATGCAATGGAGTATTATCTGCACAGTCTGGGACATAACGACACCGACGATGTGGTTCCTTTGTACATTGGCGATGATCAAACGGACGAGGACGCTTTCAAGGTAATACAAAGGAAAGGACATGGAATTGCGATACTCGTGGCATCCATTCCAAAGCACACAAACGCTTCCTACTCGTTGAAGGACCCATCGGAGGTTCTTGCTTTTTTGCTAAGGCTGGCCAGATGGAGGAACTCCACAGCCCCTTCAATTACAACCCCATCTCCTAATATTTAA

Protein sequence

MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI
Homology
BLAST of CmaCh16G000670 vs. ExPASy Swiss-Prot
Match: Q9C9S4 (Trehalose-phosphate phosphatase B OS=Arabidopsis thaliana OX=3702 GN=TPPB PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.4e-88
Identity = 155/285 (54.39%), Postives = 216/285 (75.79%), Query Frame = 0

Query: 57  SDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMRE 116
           SD  + TSW+ ++PS L  F+ I+     K+IV+FLDYDGTLSPIV+DPD+AF++ EMRE
Sbjct: 87  SDNDDKTSWIVRFPSALNMFDEIVNAAKGKQIVMFLDYDGTLSPIVEDPDKAFITHEMRE 146

Query: 117 AVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANG-----VSY 176
            V++VA  FPTAI++GRS EKV+ FV++  ++YAGSHG+DI  P ++N +NG     V +
Sbjct: 147 VVKDVASNFPTAIVTGRSIEKVRSFVQVNEIYYAGSHGMDIEGPTNEN-SNGQSNERVLF 206

Query: 177 QPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVME 236
           QPA++FLP I+++  +LEE+T+ I GA+VE+N FC+SVHFR V E+    L + V++V+ 
Sbjct: 207 QPAREFLPMIEKVVNILEEKTKWIPGAMVENNKFCLSVHFRRVDEKRWPALAEVVKSVLI 266

Query: 237 KYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKV 296
            YP   +TQG+KV+EIRPTI W+KG A+ + L SLG+ ++DDVVP+YIGDD+TDEDAFKV
Sbjct: 267 DYPKLKLTQGRKVLEIRPTIKWDKGQALNFLLKSLGYENSDDVVPVYIGDDRTDEDAFKV 326

Query: 297 IQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNST 337
           ++ +G G  ILV+ +PK TNASYSL+DPS+V  FL RL  W+  T
Sbjct: 327 LRERGQGFGILVSKVPKDTNASYSLQDPSQVNKFLERLVEWKRKT 370

BLAST of CmaCh16G000670 vs. ExPASy Swiss-Prot
Match: Q6ZGP8 (Probable trehalose-phosphate phosphatase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP4 PE=2 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.7e-88
Identity = 162/311 (52.09%), Postives = 221/311 (71.06%), Query Frame = 0

Query: 37  WRSIMGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDG 96
           W   M  S   S  R+S   SD   +T+W++++PS L  FE I  +   KKIV+F+DYDG
Sbjct: 62  WADAMRAS---SPTRSSRSASDVDEFTAWVRKHPSALSKFEEIAAKSRGKKIVMFMDYDG 121

Query: 97  TLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLD 156
           TLSPIV DPD A+MS  MR AVREVAK FPTAI+SGR R+KV+ FV L +L+YAGSHG+D
Sbjct: 122 TLSPIVADPDTAYMSDAMRAAVREVAKTFPTAIVSGRCRDKVRNFVGLSDLYYAGSHGMD 181

Query: 157 IMSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHE 216
           I  P+S+ E+     QPA +FLP I ++ + L E+T+   GA VE+N FC+SVHFR V E
Sbjct: 182 IKGPSSNPES--ALCQPASEFLPMIDEVYKTLVEKTKSTPGAKVENNKFCLSVHFRCVDE 241

Query: 217 RELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVP 276
           +    L ++V+ V+++YP   +TQG+KV+EIRP+I W+KG A+E+ L SLG  +  DV+P
Sbjct: 242 KRWNALGEQVKAVIKEYPKLKLTQGRKVLEIRPSIEWDKGKALEFLLESLGFANCGDVMP 301

Query: 277 LYIGDDQTDEDAFKVIQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNST 336
           +YIGDD+TDEDAFKV++++G G+ ILV+  PK TNASYSL+DP+EV+ FLLRL  W+  +
Sbjct: 302 VYIGDDRTDEDAFKVLRKRGQGLGILVSKCPKDTNASYSLQDPTEVMEFLLRLVEWKRKS 361

Query: 337 APSITTPSPNI 348
           + S     P +
Sbjct: 362 SSSSLMIRPRV 367

BLAST of CmaCh16G000670 vs. ExPASy Swiss-Prot
Match: Q67XC9 (Probable trehalose-phosphate phosphatase D OS=Arabidopsis thaliana OX=3702 GN=TPPD PE=2 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 6.9e-86
Identity = 153/276 (55.43%), Postives = 209/276 (75.72%), Query Frame = 0

Query: 62  YTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREV 121
           Y+SW+ Q+PS L  FE I +    K+IV+FLDYDGTLSPIV++PDRA+MS EMREAV+ V
Sbjct: 87  YSSWMAQHPSALTMFEEIAEASKGKQIVMFLDYDGTLSPIVENPDRAYMSEEMREAVKGV 146

Query: 122 AKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEAN----GVSYQPAKKF 181
           A+ FPTAI++GR R+KV+ FVKL  L+YAGSHG+DI  P+  N+ N    GV +Q A +F
Sbjct: 147 ARYFPTAIVTGRCRDKVRRFVKLPGLYYAGSHGMDIKGPSKRNKHNKNNKGVLFQAANEF 206

Query: 182 LPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFH 241
           LP I ++ + L E+ R IEGA VE+N FCVSVH+R V +++   + + V +++ +YP   
Sbjct: 207 LPMIDKVSKCLVEKMRDIEGANVENNKFCVSVHYRCVDQKDWGLVAEHVTSILSEYPKLR 266

Query: 242 VTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGH 301
           +TQG+KV+EIRPTI W+KG A+E+ L SLG  +++DV+P+YIGDD+TDEDAFKV++ KG 
Sbjct: 267 LTQGRKVLEIRPTIKWDKGKALEFLLESLGFANSNDVLPIYIGDDRTDEDAFKVLRNKGQ 326

Query: 302 GIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWR 334
           G  ILV+ IPK T+A+YSL++PSEV  FL RL  W+
Sbjct: 327 GFGILVSKIPKETSATYSLQEPSEVGEFLQRLVEWK 362

BLAST of CmaCh16G000670 vs. ExPASy Swiss-Prot
Match: Q6ZAL2 (Probable trehalose-phosphate phosphatase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=TPP6 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 7.7e-85
Identity = 158/274 (57.66%), Postives = 200/274 (72.99%), Query Frame = 0

Query: 64  SWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAK 123
           +W+ ++PS L  FE I+     KKIV+FLDYDGTLSPIVDDPD AFMS  MR AVR VAK
Sbjct: 87  AWMVRHPSALSKFEQIVAASKGKKIVMFLDYDGTLSPIVDDPDSAFMSDTMRRAVRSVAK 146

Query: 124 GFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSP-----NSDNEANGVSYQPAKKFL 183
            FPTAI+SGR R+KV EFVKL  L+YAGSHG+DI  P     ++  +A GV +QPA +FL
Sbjct: 147 HFPTAIVSGRCRDKVFEFVKLAELYYAGSHGMDIKGPAKASRHNKAKAKGVLFQPASEFL 206

Query: 184 PKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHV 243
           P I+Q+   L E T+ I GA VE+N FCVSVHFR V E+  + L   V+  ++ YP   +
Sbjct: 207 PMIEQVHDSLIERTKCIPGAKVENNKFCVSVHFRCVDEKSWSTLADIVKAELKDYPKLKL 266

Query: 244 TQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHG 303
           TQG+ V EIRPTI W+KG A+E+ L SLG  D  +V+P+YIGDD+TDEDAFKV++++G G
Sbjct: 267 TQGRMVFEIRPTIKWDKGKALEFLLESLGFADCTNVLPVYIGDDRTDEDAFKVLRKRGQG 326

Query: 304 IAILVASIPKHTNASYSLKDPSEVLAFLLRLARW 333
           I ILV+  PK TNASYSL++P+EV+ FLLRL  W
Sbjct: 327 IGILVSKYPKDTNASYSLQEPAEVMEFLLRLVEW 360

BLAST of CmaCh16G000670 vs. ExPASy Swiss-Prot
Match: Q67X99 (Probable trehalose-phosphate phosphatase E OS=Arabidopsis thaliana OX=3702 GN=TPPE PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.9e-84
Identity = 152/281 (54.09%), Postives = 203/281 (72.24%), Query Frame = 0

Query: 59  FHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAV 118
           F++ + W+K++PS L  FE I+ +   K+IV+FLDYDGTLSPIVDDPDRAFMS +MR  V
Sbjct: 70  FNSQSCWIKEHPSALNMFEEILHKSEGKQIVMFLDYDGTLSPIVDDPDRAFMSKKMRNTV 129

Query: 119 REVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDN----EANGVSYQPA 178
           R++AK FPTAI+SGR REKV  FVKL  L+YAGSHG+DI  P   +    E   +  QPA
Sbjct: 130 RKLAKCFPTAIVSGRCREKVSSFVKLTELYYAGSHGMDIKGPEQGSKYKKENQSLLCQPA 189

Query: 179 KKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYP 238
            +FLP I ++ + L E T+ I GA VE+N FC SVHFR V E + ++L  +V +V++ YP
Sbjct: 190 TEFLPVINEVYKKLVENTQSIPGAKVENNKFCASVHFRCVEENKWSDLAHQVRSVLKNYP 249

Query: 239 DFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQR 298
              +TQG+KV+EIRP I W+KG A+E+ L SLG+++  DV P+YIGDD+TDEDAFK+++ 
Sbjct: 250 KLMLTQGRKVLEIRPIIKWDKGKALEFLLESLGYDNCTDVFPIYIGDDRTDEDAFKILRD 309

Query: 299 KGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNS 336
           K  G+ ILV+   K TNASYSL++P EV+ FL RL  W+ S
Sbjct: 310 KKQGLGILVSKYAKETNASYSLQEPDEVMVFLERLVEWKQS 350

BLAST of CmaCh16G000670 vs. ExPASy TrEMBL
Match: A0A6J1J316 (Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111482936 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 5.9e-197
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60
           MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH
Sbjct: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60

Query: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120
           NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180
           VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK
Sbjct: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180

Query: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240
           IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ
Sbjct: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240

Query: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300
           GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300

Query: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI
Sbjct: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 347

BLAST of CmaCh16G000670 vs. ExPASy TrEMBL
Match: A0A6J1EA53 (Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430746 PE=3 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.1e-187
Identity = 330/347 (95.10%), Postives = 339/347 (97.69%), Query Frame = 0

Query: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60
           MNFSLLFSHVLLKGKS+LLIFLPFTFGLPALRRT+LWRSIMGLSG LSGHRNSVQGSD H
Sbjct: 1   MNFSLLFSHVLLKGKSLLLIFLPFTFGLPALRRTVLWRSIMGLSGRLSGHRNSVQGSDSH 60

Query: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120
           NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180
           VAK FPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIM+PNSDNEANGVSYQPAKKFLP+
Sbjct: 121 VAKCFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMAPNSDNEANGVSYQPAKKFLPE 180

Query: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240
           IQQIKR LEEE+RKIEGALVEDNTFCVSVHFRHVHER+L +LEKKVETVME YPDFHVTQ
Sbjct: 181 IQQIKRALEEESRKIEGALVEDNTFCVSVHFRHVHERDLGKLEKKVETVMENYPDFHVTQ 240

Query: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300
           GKKVMEIRPTINWNKGHAMEYYLH+LGHNDTDDVVPLYIGDDQTDEDAFKVIQRKG GIA
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHTLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGQGIA 300

Query: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           ILVASIPKHTNASYSLKDPSEVLAFLLRLARW NST+PSITTPSPNI
Sbjct: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWSNSTSPSITTPSPNI 347

BLAST of CmaCh16G000670 vs. ExPASy TrEMBL
Match: A0A1S3AY70 (Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103484159 PE=3 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 2.9e-135
Identity = 249/330 (75.45%), Postives = 276/330 (83.64%), Query Frame = 0

Query: 25  TFGLPALRRTLLWRSIMGLSGLLSGHRNSVQ--------GSDFHNYTSWLKQYPSGLESF 84
           TF  P     + +RS+MGL   LSG R SVQ          D  NY SWLK++P GLESF
Sbjct: 20  TFLTPFCTVAVFFRSLMGLPTPLSGDRKSVQVVPNIDNSSDDETNYNSWLKKHPCGLESF 79

Query: 85  ESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSRE 144
           E IMK L RKKIVVFLDYDGTLSPIVDDPDRAFMS EMREAVREVAK FPTAIISGRSR+
Sbjct: 80  EGIMKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVREVAKCFPTAIISGRSRD 139

Query: 145 KVKEFVKLRNLHYAGSHGLDI-MSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKI 204
           KVKEFVKL N+HYAGSHG+DI  +P SDNE + VS+QPAKKFLP IQQI+ VLEEETRKI
Sbjct: 140 KVKEFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLPVIQQIREVLEEETRKI 199

Query: 205 EGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNK 264
           EGA+VEDN FCVSVHFRHVHER+L +LEKKVETV+E+YPDFH+T GKKVMEIRPTINWNK
Sbjct: 200 EGAMVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHITSGKKVMEIRPTINWNK 259

Query: 265 GHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIAILVASIPKHTNASYS 324
           GHAMEYYLH+LGH + DDVVPLYIGDD+TDEDAFKVIQ +G GIAILV+SIPK+T ASYS
Sbjct: 260 GHAMEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVIQMRGQGIAILVSSIPKNTRASYS 319

Query: 325 LKDPSEVLAFLLRLARWRNSTAPSITTPSP 346
           LKDPS+VLAFLLRL RWR  ++ SIT P P
Sbjct: 320 LKDPSQVLAFLLRLVRWRKLSSSSITNPLP 349

BLAST of CmaCh16G000670 vs. ExPASy TrEMBL
Match: A0A5A7U3L2 (Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00200 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.1e-131
Identity = 244/315 (77.46%), Postives = 268/315 (85.08%), Query Frame = 0

Query: 41  MGLSGLLSGHRNSVQ--------GSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFL 100
           MGL   LSG R SVQ          D  NY SWLK++P GLESFE IMK L RKKIVVFL
Sbjct: 1   MGLPTPLSGDRKSVQVVPNIDNSSDDETNYNSWLKKHPCGLESFEGIMKGLKRKKIVVFL 60

Query: 101 DYDGTLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGS 160
           DYDGTLSPIVDDPDRAFMS EMREAVREVAK FPTAIISGRSR+KVKEFVKL N+HYAGS
Sbjct: 61  DYDGTLSPIVDDPDRAFMSSEMREAVREVAKCFPTAIISGRSRDKVKEFVKLSNVHYAGS 120

Query: 161 HGLDI-MSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHF 220
           HG+DI  +P SDNE + VS+QPAKKFLP IQQI+ VLEEETRKIEGA+VEDN FCVSVHF
Sbjct: 121 HGMDITTAPTSDNEVDAVSFQPAKKFLPVIQQIREVLEEETRKIEGAMVEDNRFCVSVHF 180

Query: 221 RHVHERELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDT 280
           RHVHER+L +LEKKVETV+E+YPDFH+T GKKVMEIRPTINWNKGHAMEYYLH+LGH + 
Sbjct: 181 RHVHERDLDKLEKKVETVLERYPDFHITSGKKVMEIRPTINWNKGHAMEYYLHTLGHTNE 240

Query: 281 DDVVPLYIGDDQTDEDAFK-VIQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLA 340
           DDVVPLYIGDD+TDEDAFK VIQ +G GIAILV+SIPK+T ASYSLKDPS+VLAFLLRL 
Sbjct: 241 DDVVPLYIGDDRTDEDAFKVVIQMRGQGIAILVSSIPKNTRASYSLKDPSQVLAFLLRLV 300

Query: 341 RWRNSTAPSITTPSP 346
           RWR  ++ SIT P P
Sbjct: 301 RWRKLSSSSITNPLP 315

BLAST of CmaCh16G000670 vs. ExPASy TrEMBL
Match: A0A5D3D7P4 (Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1274G00380 PE=3 SV=1)

HSP 1 Score: 440.3 bits (1131), Expect = 7.6e-120
Identity = 218/268 (81.34%), Postives = 241/268 (89.93%), Query Frame = 0

Query: 80  MKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSREKVK 139
           MK L RKKIVVFLDYDGTLSPIVDDPDRAFMS EMREAVREVAK FPTAIISGRSR+KVK
Sbjct: 1   MKGLKRKKIVVFLDYDGTLSPIVDDPDRAFMSSEMREAVREVAKCFPTAIISGRSRDKVK 60

Query: 140 EFVKLRNLHYAGSHGLDI-MSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKIEGA 199
           EFVKL N+HYAGSHG+DI  +P SDNE + VS+QPAKKFLP IQQI+ VLEEETRK+EGA
Sbjct: 61  EFVKLSNVHYAGSHGMDITTAPTSDNEVDAVSFQPAKKFLPVIQQIREVLEEETRKVEGA 120

Query: 200 LVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNKGHA 259
           +VEDN FCVSVHFRHVHER+L +LEKKVETV+E+YPDFH+T GKKVMEIRPTINWNKGHA
Sbjct: 121 MVEDNRFCVSVHFRHVHERDLDKLEKKVETVLERYPDFHITSGKKVMEIRPTINWNKGHA 180

Query: 260 MEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFK-VIQRKGHGIAILVASIPKHTNASYSLK 319
           MEYYLH+LGH + DDVVPLYIGDD+TDEDAFK VIQ +G GIAILV+SIPK+T ASYSLK
Sbjct: 181 MEYYLHTLGHTNEDDVVPLYIGDDRTDEDAFKVVIQMRGQGIAILVSSIPKNTRASYSLK 240

Query: 320 DPSEVLAFLLRLARWRNSTAPSITTPSP 346
           DPS+VLAFLLRL RWR  ++ SIT P P
Sbjct: 241 DPSQVLAFLLRLVRWRKLSSSSITNPLP 268

BLAST of CmaCh16G000670 vs. NCBI nr
Match: XP_022984752.1 (probable trehalose-phosphate phosphatase C [Cucurbita maxima])

HSP 1 Score: 696.4 bits (1796), Expect = 1.2e-196
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60
           MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH
Sbjct: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60

Query: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120
           NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180
           VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK
Sbjct: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180

Query: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240
           IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ
Sbjct: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240

Query: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300
           GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300

Query: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI
Sbjct: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 347

BLAST of CmaCh16G000670 vs. NCBI nr
Match: XP_023552932.1 (probable trehalose-phosphate phosphatase C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 677.9 bits (1748), Expect = 4.5e-191
Identity = 336/347 (96.83%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60
           MNFSLLFSHVLLKGKS+LLIFLPFTFGLPALRRT+LWRSIMGLSGLLSGHRNSVQGSDFH
Sbjct: 1   MNFSLLFSHVLLKGKSLLLIFLPFTFGLPALRRTVLWRSIMGLSGLLSGHRNSVQGSDFH 60

Query: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120
           NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180
           VAK FPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIM+PNSDNEANGVSYQPAKKFLP+
Sbjct: 121 VAKCFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMAPNSDNEANGVSYQPAKKFLPE 180

Query: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240
           IQQIKR LEEE+RKIEGALVEDNTFCVSVHFRHVHERELA+LEKKVETVMEKYPDFHVTQ
Sbjct: 181 IQQIKRALEEESRKIEGALVEDNTFCVSVHFRHVHERELAKLEKKVETVMEKYPDFHVTQ 240

Query: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300
           GKKVMEIRPTINWNKGHAMEYYLH+LGHNDTDDVVPLYIGDDQTDEDAFKVIQRKG GIA
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHTLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGQGIA 300

Query: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNST+PSITTPSPNI
Sbjct: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTSPSITTPSPNI 347

BLAST of CmaCh16G000670 vs. NCBI nr
Match: XP_022922910.1 (probable trehalose-phosphate phosphatase C [Cucurbita moschata])

HSP 1 Score: 665.6 bits (1716), Expect = 2.3e-187
Identity = 330/347 (95.10%), Postives = 339/347 (97.69%), Query Frame = 0

Query: 1   MNFSLLFSHVLLKGKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFH 60
           MNFSLLFSHVLLKGKS+LLIFLPFTFGLPALRRT+LWRSIMGLSG LSGHRNSVQGSD H
Sbjct: 1   MNFSLLFSHVLLKGKSLLLIFLPFTFGLPALRRTVLWRSIMGLSGRLSGHRNSVQGSDSH 60

Query: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120
           NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE
Sbjct: 61  NYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVRE 120

Query: 121 VAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPK 180
           VAK FPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIM+PNSDNEANGVSYQPAKKFLP+
Sbjct: 121 VAKCFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMAPNSDNEANGVSYQPAKKFLPE 180

Query: 181 IQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQ 240
           IQQIKR LEEE+RKIEGALVEDNTFCVSVHFRHVHER+L +LEKKVETVME YPDFHVTQ
Sbjct: 181 IQQIKRALEEESRKIEGALVEDNTFCVSVHFRHVHERDLGKLEKKVETVMENYPDFHVTQ 240

Query: 241 GKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIA 300
           GKKVMEIRPTINWNKGHAMEYYLH+LGHNDTDDVVPLYIGDDQTDEDAFKVIQRKG GIA
Sbjct: 241 GKKVMEIRPTINWNKGHAMEYYLHTLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGQGIA 300

Query: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           ILVASIPKHTNASYSLKDPSEVLAFLLRLARW NST+PSITTPSPNI
Sbjct: 301 ILVASIPKHTNASYSLKDPSEVLAFLLRLARWSNSTSPSITTPSPNI 347

BLAST of CmaCh16G000670 vs. NCBI nr
Match: KAG7014718.1 (putative trehalose-phosphate phosphatase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 648.3 bits (1671), Expect = 3.8e-182
Identity = 319/334 (95.51%), Postives = 329/334 (98.50%), Query Frame = 0

Query: 14  GKSVLLIFLPFTFGLPALRRTLLWRSIMGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGL 73
           GKS+LLIFLPFTFGLPALRRT+LWRSIMGLSGLLSGH NSVQGSDFHNYTSWLKQYPSGL
Sbjct: 30  GKSLLLIFLPFTFGLPALRRTVLWRSIMGLSGLLSGHLNSVQGSDFHNYTSWLKQYPSGL 89

Query: 74  ESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAKGFPTAIISGR 133
           ESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAK FPTAIISGR
Sbjct: 90  ESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAKCFPTAIISGR 149

Query: 134 SREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANGVSYQPAKKFLPKIQQIKRVLEEETR 193
           SREKVKEFVKLRNLHYAGSHGLDIM+PNSDNEANGVSYQPAKKFLP+IQQIKR LEEE+R
Sbjct: 150 SREKVKEFVKLRNLHYAGSHGLDIMAPNSDNEANGVSYQPAKKFLPEIQQIKRALEEESR 209

Query: 194 KIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHVTQGKKVMEIRPTINW 253
           KIEGALVEDNTFCVSVHFRHVHE++LA+LEKKVETVME YPDFHVTQGKKVMEIRPTINW
Sbjct: 210 KIEGALVEDNTFCVSVHFRHVHEKDLAKLEKKVETVMENYPDFHVTQGKKVMEIRPTINW 269

Query: 254 NKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHGIAILVASIPKHTNAS 313
           NKGHAMEYYLH+LGHNDTDDVVPLYIGDDQTDEDAFKVIQRKG GIAILVASIPKHTNAS
Sbjct: 270 NKGHAMEYYLHTLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGQGIAILVASIPKHTNAS 329

Query: 314 YSLKDPSEVLAFLLRLARWRNSTAPSITTPSPNI 348
           YSLKDPSEVLAFLLRLARWRNST+PSITTPSPNI
Sbjct: 330 YSLKDPSEVLAFLLRLARWRNSTSPSITTPSPNI 363

BLAST of CmaCh16G000670 vs. NCBI nr
Match: KAG6576668.1 (putative trehalose-phosphate phosphatase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 547.4 bits (1409), Expect = 9.2e-152
Identity = 272/307 (88.60%), Postives = 280/307 (91.21%), Query Frame = 0

Query: 41  MGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSP 100
           MGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSP
Sbjct: 1   MGLSGLLSGHRNSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSP 60

Query: 101 IVDDPDRAFMSPEMREAVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSP 160
           IVDDPDRAFMSPE                        VKEFVKLRNLHYAGSHGLDIM+P
Sbjct: 61  IVDDPDRAFMSPE------------------------VKEFVKLRNLHYAGSHGLDIMAP 120

Query: 161 NSDNEANGVSYQPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELA 220
           NSDNEANGVSYQPAKKFLP+IQQIKR LEEE+RKIEGALVEDNTFCVSVHFRHVHE++LA
Sbjct: 121 NSDNEANGVSYQPAKKFLPEIQQIKRALEEESRKIEGALVEDNTFCVSVHFRHVHEKDLA 180

Query: 221 ELEKKVETVMEKYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIG 280
           +LEKKVETVME YPDFHVTQGKKVMEIRPTINWNKGHAMEYYLH+LGHNDTDDVVPLYIG
Sbjct: 181 KLEKKVETVMENYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHTLGHNDTDDVVPLYIG 240

Query: 281 DDQTDEDAFKVIQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTAPSI 340
           DDQTDEDAFKVIQRKG GIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNST+PSI
Sbjct: 241 DDQTDEDAFKVIQRKGQGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNSTSPSI 283

Query: 341 TTPSPNI 348
           TTPSPNI
Sbjct: 301 TTPSPNI 283

BLAST of CmaCh16G000670 vs. TAIR 10
Match: AT1G78090.1 (trehalose-6-phosphate phosphatase )

HSP 1 Score: 326.2 bits (835), Expect = 3.1e-89
Identity = 155/285 (54.39%), Postives = 216/285 (75.79%), Query Frame = 0

Query: 57  SDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMRE 116
           SD  + TSW+ ++PS L  F+ I+     K+IV+FLDYDGTLSPIV+DPD+AF++ EMRE
Sbjct: 87  SDNDDKTSWIVRFPSALNMFDEIVNAAKGKQIVMFLDYDGTLSPIVEDPDKAFITHEMRE 146

Query: 117 AVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEANG-----VSY 176
            V++VA  FPTAI++GRS EKV+ FV++  ++YAGSHG+DI  P ++N +NG     V +
Sbjct: 147 VVKDVASNFPTAIVTGRSIEKVRSFVQVNEIYYAGSHGMDIEGPTNEN-SNGQSNERVLF 206

Query: 177 QPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVME 236
           QPA++FLP I+++  +LEE+T+ I GA+VE+N FC+SVHFR V E+    L + V++V+ 
Sbjct: 207 QPAREFLPMIEKVVNILEEKTKWIPGAMVENNKFCLSVHFRRVDEKRWPALAEVVKSVLI 266

Query: 237 KYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKV 296
            YP   +TQG+KV+EIRPTI W+KG A+ + L SLG+ ++DDVVP+YIGDD+TDEDAFKV
Sbjct: 267 DYPKLKLTQGRKVLEIRPTIKWDKGQALNFLLKSLGYENSDDVVPVYIGDDRTDEDAFKV 326

Query: 297 IQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNST 337
           ++ +G G  ILV+ +PK TNASYSL+DPS+V  FL RL  W+  T
Sbjct: 327 LRERGQGFGILVSKVPKDTNASYSLQDPSQVNKFLERLVEWKRKT 370

BLAST of CmaCh16G000670 vs. TAIR 10
Match: AT1G35910.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 318.9 bits (816), Expect = 4.9e-87
Identity = 153/276 (55.43%), Postives = 209/276 (75.72%), Query Frame = 0

Query: 62  YTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREV 121
           Y+SW+ Q+PS L  FE I +    K+IV+FLDYDGTLSPIV++PDRA+MS EMREAV+ V
Sbjct: 87  YSSWMAQHPSALTMFEEIAEASKGKQIVMFLDYDGTLSPIVENPDRAYMSEEMREAVKGV 146

Query: 122 AKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDNEAN----GVSYQPAKKF 181
           A+ FPTAI++GR R+KV+ FVKL  L+YAGSHG+DI  P+  N+ N    GV +Q A +F
Sbjct: 147 ARYFPTAIVTGRCRDKVRRFVKLPGLYYAGSHGMDIKGPSKRNKHNKNNKGVLFQAANEF 206

Query: 182 LPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFH 241
           LP I ++ + L E+ R IEGA VE+N FCVSVH+R V +++   + + V +++ +YP   
Sbjct: 207 LPMIDKVSKCLVEKMRDIEGANVENNKFCVSVHYRCVDQKDWGLVAEHVTSILSEYPKLR 266

Query: 242 VTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGH 301
           +TQG+KV+EIRPTI W+KG A+E+ L SLG  +++DV+P+YIGDD+TDEDAFKV++ KG 
Sbjct: 267 LTQGRKVLEIRPTIKWDKGKALEFLLESLGFANSNDVLPIYIGDDRTDEDAFKVLRNKGQ 326

Query: 302 GIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWR 334
           G  ILV+ IPK T+A+YSL++PSEV  FL RL  W+
Sbjct: 327 GFGILVSKIPKETSATYSLQEPSEVGEFLQRLVEWK 362

BLAST of CmaCh16G000670 vs. TAIR 10
Match: AT2G22190.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 2.1e-85
Identity = 152/281 (54.09%), Postives = 203/281 (72.24%), Query Frame = 0

Query: 59  FHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAV 118
           F++ + W+K++PS L  FE I+ +   K+IV+FLDYDGTLSPIVDDPDRAFMS +MR  V
Sbjct: 70  FNSQSCWIKEHPSALNMFEEILHKSEGKQIVMFLDYDGTLSPIVDDPDRAFMSKKMRNTV 129

Query: 119 REVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNSDN----EANGVSYQPA 178
           R++AK FPTAI+SGR REKV  FVKL  L+YAGSHG+DI  P   +    E   +  QPA
Sbjct: 130 RKLAKCFPTAIVSGRCREKVSSFVKLTELYYAGSHGMDIKGPEQGSKYKKENQSLLCQPA 189

Query: 179 KKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYP 238
            +FLP I ++ + L E T+ I GA VE+N FC SVHFR V E + ++L  +V +V++ YP
Sbjct: 190 TEFLPVINEVYKKLVENTQSIPGAKVENNKFCASVHFRCVEENKWSDLAHQVRSVLKNYP 249

Query: 239 DFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQR 298
              +TQG+KV+EIRP I W+KG A+E+ L SLG+++  DV P+YIGDD+TDEDAFK+++ 
Sbjct: 250 KLMLTQGRKVLEIRPIIKWDKGKALEFLLESLGYDNCTDVFPIYIGDDRTDEDAFKILRD 309

Query: 299 KGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWRNS 336
           K  G+ ILV+   K TNASYSL++P EV+ FL RL  W+ S
Sbjct: 310 KKQGLGILVSKYAKETNASYSLQEPDEVMVFLERLVEWKQS 350

BLAST of CmaCh16G000670 vs. TAIR 10
Match: AT5G65140.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 309.3 bits (791), Expect = 3.9e-84
Identity = 152/287 (52.96%), Postives = 205/287 (71.43%), Query Frame = 0

Query: 52  NSVQGSDFHNYTSWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMS 111
           +S    + HN  SW+K++PS L  FE I++E   K+IV+FLDYDGTLSPIVDDPDRAFM+
Sbjct: 82  SSFSSEEEHN--SWIKRHPSALNMFERIIEEARGKQIVMFLDYDGTLSPIVDDPDRAFMT 141

Query: 112 PEMREAVREVAKGFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSPNS-----DNEA 171
            +MR  V+++AK FPT+I++GR  +KV  FVKL  L+YAGSHG+DI  P       + + 
Sbjct: 142 SKMRRTVKKMAKCFPTSIVTGRCIDKVYSFVKLAELYYAGSHGMDIKGPTKGFSRYNKDK 201

Query: 172 NGVSYQPAKKFLPKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKV 231
             V YQPA  FLP I ++ + L E+T+   GA VE+N FC+SVHFR V E++ +EL  KV
Sbjct: 202 PSVLYQPAGDFLPMIDEVYKQLVEKTKSTPGAKVENNKFCLSVHFRCVDEKKWSELASKV 261

Query: 232 ETVMEKYPDFHVTQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDE 291
            +V++ YP   ++QG+KV EIRP I WNKG A+E+ L SLG  + +DV P+YIGDD+TDE
Sbjct: 262 RSVVKNYPTLKLSQGRKVFEIRPIIKWNKGKALEFLLESLGFENCNDVFPIYIGDDKTDE 321

Query: 292 DAFKVIQRKGHGIAILVASIPKHTNASYSLKDPSEVLAFLLRLARWR 334
           DAFK+++ +G G  ILV+  PK T+ASYSL+DP EV+ FL RL  W+
Sbjct: 322 DAFKLLRGRGQGFGILVSKFPKDTSASYSLQDPPEVMNFLGRLVEWK 366

BLAST of CmaCh16G000670 vs. TAIR 10
Match: AT5G10100.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 5.6e-83
Identity = 149/275 (54.18%), Postives = 199/275 (72.36%), Query Frame = 0

Query: 64  SWLKQYPSGLESFESIMKELPRKKIVVFLDYDGTLSPIVDDPDRAFMSPEMREAVREVAK 123
           SW+ Q+PS LE FE IM+    K+IV+FLDYDGTLSPIVDDPD+AFMS +MR  V+++AK
Sbjct: 89  SWIMQHPSALEKFEQIMEASRGKQIVMFLDYDGTLSPIVDDPDKAFMSSKMRRTVKKLAK 148

Query: 124 GFPTAIISGRSREKVKEFVKLRNLHYAGSHGLDIMSP-----NSDNEANGVSYQPAKKFL 183
            FPTAI++GR  +KV  FVKL  L+YAGSHG+DI  P             + YQPA  +L
Sbjct: 149 CFPTAIVTGRCIDKVYNFVKLAELYYAGSHGMDIKGPAKGFSRHKRVKQSLLYQPANDYL 208

Query: 184 PKIQQIKRVLEEETRKIEGALVEDNTFCVSVHFRHVHERELAELEKKVETVMEKYPDFHV 243
           P I ++ R L E+T+   GA VE++ FC SVHFR V E++ +EL  +V +V++K+P   +
Sbjct: 209 PMIDEVYRQLLEKTKSTPGAKVENHKFCASVHFRCVDEKKWSELVLQVRSVLKKFPTLQL 268

Query: 244 TQGKKVMEIRPTINWNKGHAMEYYLHSLGHNDTDDVVPLYIGDDQTDEDAFKVIQRKGHG 303
           TQG+KV EIRP I W+KG A+E+ L SLG  +T++V P+YIGDD+TDEDAFK+++ +G G
Sbjct: 269 TQGRKVFEIRPMIEWDKGKALEFLLESLGFGNTNNVFPVYIGDDRTDEDAFKMLRDRGEG 328

Query: 304 IAILVASIPKHTNASYSLKDPSEVLAFLLRLARWR 334
             ILV+  PK T+ASYSL+DPSEV+ FL RL  W+
Sbjct: 329 FGILVSKFPKDTDASYSLQDPSEVMDFLRRLVEWK 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9S44.4e-8854.39Trehalose-phosphate phosphatase B OS=Arabidopsis thaliana OX=3702 GN=TPPB PE=1 S... [more]
Q6ZGP89.7e-8852.09Probable trehalose-phosphate phosphatase 4 OS=Oryza sativa subsp. japonica OX=39... [more]
Q67XC96.9e-8655.43Probable trehalose-phosphate phosphatase D OS=Arabidopsis thaliana OX=3702 GN=TP... [more]
Q6ZAL27.7e-8557.66Probable trehalose-phosphate phosphatase 6 OS=Oryza sativa subsp. japonica OX=39... [more]
Q67X992.9e-8454.09Probable trehalose-phosphate phosphatase E OS=Arabidopsis thaliana OX=3702 GN=TP... [more]
Match NameE-valueIdentityDescription
A0A6J1J3165.9e-197100.00Trehalose 6-phosphate phosphatase OS=Cucurbita maxima OX=3661 GN=LOC111482936 PE... [more]
A0A6J1EA531.1e-18795.10Trehalose 6-phosphate phosphatase OS=Cucurbita moschata OX=3662 GN=LOC111430746 ... [more]
A0A1S3AY702.9e-13575.45Trehalose 6-phosphate phosphatase OS=Cucumis melo OX=3656 GN=LOC103484159 PE=3 S... [more]
A0A5A7U3L21.1e-13177.46Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5D3D7P47.6e-12081.34Trehalose 6-phosphate phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
Match NameE-valueIdentityDescription
XP_022984752.11.2e-196100.00probable trehalose-phosphate phosphatase C [Cucurbita maxima][more]
XP_023552932.14.5e-19196.83probable trehalose-phosphate phosphatase C [Cucurbita pepo subsp. pepo][more]
XP_022922910.12.3e-18795.10probable trehalose-phosphate phosphatase C [Cucurbita moschata][more]
KAG7014718.13.8e-18295.51putative trehalose-phosphate phosphatase 4, partial [Cucurbita argyrosperma subs... [more]
KAG6576668.19.2e-15288.60putative trehalose-phosphate phosphatase 4, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
AT1G78090.13.1e-8954.39trehalose-6-phosphate phosphatase [more]
AT1G35910.14.9e-8755.43Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT2G22190.12.1e-8554.09Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G65140.13.9e-8452.96Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT5G10100.15.6e-8354.18Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 181..201
NoneNo IPR availableCOILSCoilCoilcoord: 212..232
NoneNo IPR availableGENE3D3.30.70.1020coord: 174..250
e-value: 4.1E-51
score: 175.6
NoneNo IPR availablePANTHERPTHR43768:SF24TREHALOSE 6-PHOSPHATE PHOSPHATASEcoord: 58..341
NoneNo IPR availableCDDcd01627HAD_TPPcoord: 89..324
e-value: 4.4648E-64
score: 201.364
IPR006379HAD-superfamily hydrolase, subfamily IIBTIGRFAMTIGR01484TIGR01484coord: 90..295
e-value: 4.1E-22
score: 77.3
IPR003337Trehalose-phosphatasePFAMPF02358Trehalose_PPasecoord: 91..320
e-value: 4.8E-72
score: 241.9
IPR003337Trehalose-phosphataseTIGRFAMTIGR00685TIGR00685coord: 86..330
e-value: 4.1E-52
score: 175.5
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 90..316
e-value: 4.1E-51
score: 175.6
IPR044651Trehalose 6-phosphate OTSB-likePANTHERPTHR43768TREHALOSE 6-PHOSPHATE PHOSPHATASEcoord: 58..341
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 89..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh16G000670.1CmaCh16G000670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0005992 trehalose biosynthetic process
molecular_function GO:0004805 trehalose-phosphatase activity
molecular_function GO:0003824 catalytic activity