CmaCh15G013140 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G013140
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionmethionine gamma-lyase-like
LocationCma_Chr15: 8261263 .. 8263515 (+)
RNA-Seq ExpressionCmaCh15G013140
SyntenyCmaCh15G013140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCTACAAAACTTGCCCTCCATATCTCAATCTCAAATTCAAAACCCTTCATTCTAATTGAGATGTCCATGGCTGAAGTAAAAATGTTGTGGGACGTCAAGAACAAGAAGTTGAAGCCCACAGCTCGGCTGCCGCCACTGCCCGTGACATGGCGGCCGGAGGAGGAGGAGGATCCAGCTGCTTCTCTGGCAGCGGTCCGGCCGGAGTTCGGAGAGCATGGGGGTGTGAACCTATCAATCGAAAACTCGATATCGTTCACGGTGATGGAGGCGGAAACTATGCGTAAGATGTTCGAAGGAGAGTTGGGACCGGAACAAGACTGCTTCATCTACAGCCGCCATTTCAACCCGACCGTCATGGCCCTCGGCCGACAGATGGCGGCGCTTGAAGGGACTGAGGCGGCGTATTGCACGTCCAGTGGGATGGCGGCTGTTTCATCTGTTTTGCTCCAGCTTCTCAGCTCCGGTGGCCATATTGTCGCCTCCAGGACGCTCTATGGCGGCACTCACGCGTTGCTCTCCCATTTCCTGCCGAGGGTCTGCAACATTACGACGTCGTTTGTGGATGTGAAGGACCATGCCATGGTTAGAGATGCCATGGTGAAGGGCAAGACGAAGGTACACCTTGAACCAATTTGATTTTTTTTTTTTTTTAATTTCTAAGAAAATTGTAATTAATGATAAAAATAATTAATATTTTACACAAATAAATCAATTTCTGTCCTAGCCCAATAAATATTGTCCTATTTCGACTTTTCATCGACGTTTTTAAAAAGCTTCTACTTTGGAGAGGTTTCCACACCCAGGATCCAGCATCGACACTTGTTCCCGTCAATCAATGTGGGACCGCCCAATGCACCCCTCTTCAGGCCCCAACGTCCTTGTTGTCACTCCACCTCGTGTCCACCCCCTTCGAGTCTCAGCCTCCTTACTAGCACATCGCCCGGTGTCTGACTCTGATACTATTTGTAACAGCCCAAACCTACCGCTAGCAGATATTGTCCTCTTTGAGTTTTTCCTTTACGGCTTCTCCTCAAAAATTTTCACACCCTTATAAATAAGGATTTCACAGAAAGAGAGAGGCTGTGCTCATAAATATTTATTATTGATAGATTATTTTACGTCTATTAATGAGGGAAAAAAACGATACTATATGAGTTTAAACACAAGTTCTAACTCTCATAACATGTTAAATTAACCCAAAGTCATAAGTAATTAGATTACGGTAAATTTAATCTTTTATTTTTATTCTAAATCTAGGTGTTGTATTTCGAGACGGTGGCGAACCCAACACTAACCGTGGCAAACATCCCCGAGCTCAGTCGGATGGCGCATGAGAAGGGAGTGACAGTGGTGGTCGATAATACATTCTCGCCAATGATAATCTCACCGACGAGGCTCGGTGCTGACGTCGTGGTTCATAGCTGCTCCAAGTTCATCAGTGGCGGAGCCGACATAATTGCCGGTATAATACATGTTATTTAGTCACTAAACATGATTTCAAAAATTTTAATTTACCTAATCATAATTGTGAGATCCAACGTCGTTTTTTGTAGGTGCGGTTTGTGGGAAGAAAAAATTAGTGAATTCCATGATGGACCTTCACCAAGGCACCCTAATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCAGAACTCTCTCAAAGAGTCCCCCATTTAGCCCTCCGAATGAAAGAGCACTCCCACAGGACTTTAGTTTTTGCTCAAAGAATGAAGAATCTTGGCCTCAAAGTCATATACCCTGGCCTCAAAGATCACCCTGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTCTATGCATCGACATGGGGACCGAAGAGCGAGCCAATAGACTCATGAATCTCCTTCAAAACCATACTCATTTCGGTTTTTTAGCCGTAAGCTTGGGCTACTTCGAGACTCTCATATCGTGCTCAGGTAGAGTCAAGTCAATTCAATCAACTCAAAAGTCTAACCTAACGAGTTCATGTTTTAATTATTTGTTTGTATTTTCGATAGGTAGTAGCACGAGTAGCGAGATGAACGTCGAGGAGAAGGAGCTGGCGGGCATTTCTCCGGGATTGATTAGGATGTCTGTTGCATATGTTGGAACCCTAGAGCAGAAATGGAGCCAATTGGAGAAGGCATTACTAAAGCTGAATATTTGAAGGATTCAGCTCTGTTAAGGTTGTTAAAGTGTGTTGGAAAGAACAAGCCTGTTGTTTTAG

mRNA sequence

CCCCTACAAAACTTGCCCTCCATATCTCAATCTCAAATTCAAAACCCTTCATTCTAATTGAGATGTCCATGGCTGAAGTAAAAATGTTGTGGGACGTCAAGAACAAGAAGTTGAAGCCCACAGCTCGGCTGCCGCCACTGCCCGTGACATGGCGGCCGGAGGAGGAGGAGGATCCAGCTGCTTCTCTGGCAGCGGTCCGGCCGGAGTTCGGAGAGCATGGGGGTGTGAACCTATCAATCGAAAACTCGATATCGTTCACGGTGATGGAGGCGGAAACTATGCGTAAGATGTTCGAAGGAGAGTTGGGACCGGAACAAGACTGCTTCATCTACAGCCGCCATTTCAACCCGACCGTCATGGCCCTCGGCCGACAGATGGCGGCGCTTGAAGGGACTGAGGCGGCGTATTGCACGTCCAGTGGGATGGCGGCTGTTTCATCTGTTTTGCTCCAGCTTCTCAGCTCCGGTGGCCATATTGTCGCCTCCAGGACGCTCTATGGCGGCACTCACGCGTTGCTCTCCCATTTCCTGCCGAGGGTCTGCAACATTACGACGTCGTTTGTGGATGTGAAGGACCATGCCATGGTTAGAGATGCCATGGTGAAGGGCAAGACGAAGGTGTTGTATTTCGAGACGGTGGCGAACCCAACACTAACCGTGGCAAACATCCCCGAGCTCAGTCGGATGGCGCATGAGAAGGGAGTGACAGTGGTGGTCGATAATACATTCTCGCCAATGATAATCTCACCGACGAGGCTCGGTGCTGACGTCGTGGTTCATAGCTGCTCCAAGTTCATCAGTGGCGGAGCCGACATAATTGCCGGTGCGGTTTGTGGGAAGAAAAAATTAGTGAATTCCATGATGGACCTTCACCAAGGCACCCTAATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCAGAACTCTCTCAAAGAGTCCCCCATTTAGCCCTCCGAATGAAAGAGCACTCCCACAGGACTTTAGTTTTTGCTCAAAGAATGAAGAATCTTGGCCTCAAAGTCATATACCCTGGCCTCAAAGATCACCCTGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTCTATGCATCGACATGGGGACCGAAGAGCGAGCCAATAGACTCATGAATCTCCTTCAAAACCATACTCATTTCGGTTTTTTAGCCGTAAGCTTGGGCTACTTCGAGACTCTCATATCGTGCTCAGGTAGTAGCACGAGTAGCGAGATGAACGTCGAGGAGAAGGAGCTGGCGGGCATTTCTCCGGGATTGATTAGGATGTCTGTTGCATATGTTGGAACCCTAGAGCAGAAATGGAGCCAATTGGAGAAGGCATTACTAAAGCTGAATATTTGAAGGATTCAGCTCTGTTAAGGTTGTTAAAGTGTGTTGGAAAGAACAAGCCTGTTGTTTTAG

Coding sequence (CDS)

ATGTCCATGGCTGAAGTAAAAATGTTGTGGGACGTCAAGAACAAGAAGTTGAAGCCCACAGCTCGGCTGCCGCCACTGCCCGTGACATGGCGGCCGGAGGAGGAGGAGGATCCAGCTGCTTCTCTGGCAGCGGTCCGGCCGGAGTTCGGAGAGCATGGGGGTGTGAACCTATCAATCGAAAACTCGATATCGTTCACGGTGATGGAGGCGGAAACTATGCGTAAGATGTTCGAAGGAGAGTTGGGACCGGAACAAGACTGCTTCATCTACAGCCGCCATTTCAACCCGACCGTCATGGCCCTCGGCCGACAGATGGCGGCGCTTGAAGGGACTGAGGCGGCGTATTGCACGTCCAGTGGGATGGCGGCTGTTTCATCTGTTTTGCTCCAGCTTCTCAGCTCCGGTGGCCATATTGTCGCCTCCAGGACGCTCTATGGCGGCACTCACGCGTTGCTCTCCCATTTCCTGCCGAGGGTCTGCAACATTACGACGTCGTTTGTGGATGTGAAGGACCATGCCATGGTTAGAGATGCCATGGTGAAGGGCAAGACGAAGGTGTTGTATTTCGAGACGGTGGCGAACCCAACACTAACCGTGGCAAACATCCCCGAGCTCAGTCGGATGGCGCATGAGAAGGGAGTGACAGTGGTGGTCGATAATACATTCTCGCCAATGATAATCTCACCGACGAGGCTCGGTGCTGACGTCGTGGTTCATAGCTGCTCCAAGTTCATCAGTGGCGGAGCCGACATAATTGCCGGTGCGGTTTGTGGGAAGAAAAAATTAGTGAATTCCATGATGGACCTTCACCAAGGCACCCTAATGCTTCTTGGGCCAACAATGAACGCCAAAGTTGCATCAGAACTCTCTCAAAGAGTCCCCCATTTAGCCCTCCGAATGAAAGAGCACTCCCACAGGACTTTAGTTTTTGCTCAAAGAATGAAGAATCTTGGCCTCAAAGTCATATACCCTGGCCTCAAAGATCACCCTGACCACCATCTTCTAAGCTCCATAGCCAACAAAGACTACGGCTACGGCGGAGTTCTATGCATCGACATGGGGACCGAAGAGCGAGCCAATAGACTCATGAATCTCCTTCAAAACCATACTCATTTCGGTTTTTTAGCCGTAAGCTTGGGCTACTTCGAGACTCTCATATCGTGCTCAGGTAGTAGCACGAGTAGCGAGATGAACGTCGAGGAGAAGGAGCTGGCGGGCATTTCTCCGGGATTGATTAGGATGTCTGTTGCATATGTTGGAACCCTAGAGCAGAAATGGAGCCAATTGGAGAAGGCATTACTAAAGCTGAATATTTGA

Protein sequence

MSMAEVKMLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKLNI
Homology
BLAST of CmaCh15G013140 vs. ExPASy Swiss-Prot
Match: Q9SGU9 (Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.2e-175
Identity = 297/402 (73.88%), Postives = 353/402 (87.81%), Query Frame = 0

Query: 35  EEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHF 94
           + DPAA++A +R EFGEHGGVN+SIE S +FTVME +TMR+MF GELGP+ D ++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 95  NPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSH 154
           NPTV+ L RQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 155 FLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGV 214
           FLPR CNITTSFVD+ DH  V +A+V+G+T+VLYFE+VANPTLTVA+IPELSRMAHEKGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 215 TVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTL 274
           TVVVDNTF+PM++SP +LGADVVVHS SKFISGGADIIAGAVCG + LV  MMDL  G+L
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSL 279

Query: 275 MLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHL 334
           MLLGPTMNAKVA ELS+R+PHL LRM+EHSHR  V+A+RM++LG+KVIYPGL+ HP H L
Sbjct: 280 MLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKL 339

Query: 335 LSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTS 394
              + N+DYGYGG+L IDM TEE+AN+LM  LQN T FGF+AVSLGY+ETL+SCSGSSTS
Sbjct: 340 FKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTS 399

Query: 395 SEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           SE++  +KE AGISPGL+RMSV YVGTLEQKW+Q EKA L++
Sbjct: 400 SELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of CmaCh15G013140 vs. ExPASy Swiss-Prot
Match: Q8L0X4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 GN=mgl PE=1 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 5.0e-49
Identity = 145/392 (36.99%), Postives = 217/392 (55.36%), Query Frame = 0

Query: 52  HGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGT 111
           +G + + I  + +F    AE   + F      E+  +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 112 EAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKD 171
           EAA  TSSGM A+SS L  +L +G H+V  +TLYG T AL+ H L R   I  +FVD  +
Sbjct: 82  EAAVATSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMCHGLTRF-GIEVTFVDTSN 141

Query: 172 HAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGVT-VVVDNTF-SPMIISP 231
              V++AM K  T+V+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P
Sbjct: 142 LDEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKLAHTNPNTLVIVDNTFATPYMQKP 201

Query: 232 TRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASEL 291
            +LGAD+VVHS +K+I+G  D+IAG V   K+L + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGADIVVHSVTKYINGHGDVIAGLVITNKELADQIRFI--GLKDMTGAVLGPQDAYYI 261

Query: 292 SQRVPHLALRMKEH---SHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDY-GY 351
            + +    +RM+ H   + + + F  +   +  +V YPGL+ HP H     IA K    +
Sbjct: 262 IRGMKTFEIRMERHCKNAKKVVEFLNKHPKIE-RVYYPGLETHPGH----EIAKKQMKDF 321

Query: 352 GGVLCIDM-GTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKEL 411
           G ++  ++ G  E    L+N L+       LAVSLG  ETLI    S T S    EE+E 
Sbjct: 322 GAMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREA 381

Query: 412 AGISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           AGI+ GL+R+SV  +  +E   + LE+ L K+
Sbjct: 382 AGITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of CmaCh15G013140 vs. ExPASy Swiss-Prot
Match: Q8RDT4 (L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355) OX=190304 GN=FN1419 PE=1 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.6e-47
Identity = 141/391 (36.06%), Postives = 216/391 (55.24%), Query Frame = 0

Query: 52  HGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGT 111
           +G + + I  + +F    AE   + F      E+  +IY+R  NPT   L  ++AALE  
Sbjct: 22  YGTLAMPIYQTSTFIFDSAEQGGRRF----ALEEAGYIYTRLGNPTTTVLENKIAALEEG 81

Query: 112 EAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKD 171
           EA    SSGM A+SS L  +L +G H+V  +TLYG T AL++H L R   +  +FVD  +
Sbjct: 82  EAGIAMSSGMGAISSTLWTVLKAGDHVVTDKTLYGCTFALMNHGLTRF-GVEVTFVDTSN 141

Query: 172 HAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGVT-VVVDNTF-SPMIISP 231
              V++AM K  T+V+Y ET ANP L + ++  LS++AH    T V+VDNTF +P +  P
Sbjct: 142 LEEVKNAM-KKNTRVVYLETPANPNLKIVDLEALSKIAHTNPNTLVIVDNTFATPYMQKP 201

Query: 232 TRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASEL 291
            +LG D+VVHS +K+++G  D+IAG V  +++L + +  +  G   + G  +  + A  +
Sbjct: 202 LKLGVDIVVHSATKYLNGHGDVIAGLVVTRQELADQIRFV--GLKDMTGAVLGPQEAYYI 261

Query: 292 SQRVPHLALRMKEH--SHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDY-GYG 351
            + +    +RM+ H  + RT+V          KV YPGL+ HP +     IA K    +G
Sbjct: 262 IRGLKTFEIRMERHCKNARTIVDFLNKHPKVEKVYYPGLETHPGY----EIAKKQMKDFG 321

Query: 352 GVLCIDM-GTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELA 411
            ++  ++ G  E    L+N L+       LAVSLG  ETLI    S T S    EE+E+A
Sbjct: 322 AMISFELKGGFEAGKTLLNNLK----LCSLAVSLGDTETLIQHPASMTHSPYTKEEREVA 381

Query: 412 GISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           GI+ GL+R+SV  +  +E   + LE+ L K+
Sbjct: 382 GITDGLVRLSVG-LENVEDIIADLEQGLEKI 395

BLAST of CmaCh15G013140 vs. ExPASy Swiss-Prot
Match: Q7MX71 (L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=mgl PE=1 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.7e-44
Identity = 133/364 (36.54%), Postives = 200/364 (54.95%), Query Frame = 0

Query: 59  IENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTS 118
           I  + +F    AE   + F G    E+D +IY+R  NP    +  ++A LEG EAA   S
Sbjct: 32  IYQTSTFVFDTAEQGGRRFAG----EEDGYIYTRLGNPNCTQVEEKLAMLEGGEAAASAS 91

Query: 119 SGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDA 178
           SG+ A+SS +   + +G HIVA +TLYG T A L+H L R   +  + VD + H    +A
Sbjct: 92  SGIGAISSAIWVCVKAGDHIVAGKTLYGCTFAFLTHGLSRY-GVEVTLVDTR-HPEEVEA 151

Query: 179 MVKGKTKVLYFETVANPTLTVANIPELSRMAHE-KGVTVVVDNTF-SPMIISPTRLGADV 238
            ++  TK++Y ET ANP + + +I  +  +AH+ +GV V+VDNT+ +P I  P  LGAD+
Sbjct: 152 AIRPNTKLVYLETPANPNMYLTDIKAVCDIAHKHEGVRVMVDNTYCTPYICRPLELGADI 211

Query: 239 VVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHL 298
           VVHS +K+++G  D+IAG V GK+  +  +  +  G   L G  M+   A  +S+ +  L
Sbjct: 212 VVHSATKYLNGHGDVIAGFVVGKEDYIKEVKLV--GVKDLTGANMSPFDAYLISRGMKTL 271

Query: 299 ALRMKEHSHRTLVFAQRM-KNLGLKVIY-PGLKDHPDHHLLSSIANKDYGY-GGVLCIDM 358
            +RM++H       A+ + K+  ++ +Y PGL   P + L    A K     G ++  ++
Sbjct: 272 QIRMEQHCRNAQTVAEFLEKHPAVEAVYFPGLPSFPQYEL----AKKQMALPGAMIAFEV 331

Query: 359 -GTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLI 417
            G  E   +LMN L    H   LAVSLG  ETLI    S T S    EE+  + IS GL+
Sbjct: 332 KGGCEAGKKLMNNL----HLCSLAVSLGDTETLIQHPASMTHSPYTPEERAASDISEGLV 379

BLAST of CmaCh15G013140 vs. ExPASy Swiss-Prot
Match: Q73KL7 (L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104) OX=243275 GN=megL PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 3.2e-43
Identity = 131/391 (33.50%), Postives = 207/391 (52.94%), Query Frame = 0

Query: 51  EHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEG 110
           ++G +   I  + +F    AE   + F      E++ +IY+R  NPT   +  ++A LE 
Sbjct: 24  KYGALATPIYQTSTFAFDSAEQGGRRF----ALEEEGYIYTRLGNPTTTVVEEKLACLEN 83

Query: 111 TEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVK 170
            EA    SSG+ AV+S +  ++++G HIVA +TLYG T A L+H L R   +  +FVD +
Sbjct: 84  GEACMSASSGIGAVTSCIWSIVNAGDHIVAGKTLYGCTFAFLNHGLSRF-GVDVTFVDTR 143

Query: 171 DHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKG--VTVVVDNTF-SPMII 230
           D   V+ A+ K  TK++Y ET ANP + + +I  +S++AH       V+VDNT+ +P + 
Sbjct: 144 DPENVKKAL-KPNTKIVYLETPANPNMYLCDIAAVSKIAHAHNPECKVIVDNTYMTPYLQ 203

Query: 231 SPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVAS 290
            P  LGADVV+HS +K+++G  D+IAG V GKK+ ++ +  +  G   + G T+    A 
Sbjct: 204 RPLDLGADVVLHSATKYLNGHGDVIAGFVVGKKEFIDQVRFV--GVKDMTGSTLGPFEAY 263

Query: 291 ELSQRVPHLALRMKEHSHRTLVFAQRMKNLGL--KVIYPGLKDHPDHHLLSSIANKDYGY 350
            + + +  L +RM++H       A+ ++       + +PGLK  P + L    A K    
Sbjct: 264 LIGRGMKTLDIRMEKHCANAQKVAEFLEKHPAVESIAFPGLKSFPQYEL----AKKQMKL 323

Query: 351 GGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELA 410
            G +     T +        L N   F  +AVSLG  ETLI    S T S    EE+  +
Sbjct: 324 CGAMI--AFTVKGGLEAGKTLINSVKFATIAVSLGDAETLIQHPASMTHSPYTPEERAAS 383

Query: 411 GISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
            I+ GL+R+SV  +   E   + L++AL KL
Sbjct: 384 DIAEGLVRLSVG-LEDAEDIIADLKQALDKL 399

BLAST of CmaCh15G013140 vs. ExPASy TrEMBL
Match: A0A6J1I2P5 (methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 3.7e-244
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 8   MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV 67
           MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV
Sbjct: 1   MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV 60

Query: 68  MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV 127
           MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV
Sbjct: 61  MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV 120

Query: 128 LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL 187
           LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL
Sbjct: 121 LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL 180

Query: 188 YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG 247
           YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG
Sbjct: 181 YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG 240

Query: 248 GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT 307
           GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT
Sbjct: 241 GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT 300

Query: 308 LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ 367
           LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ
Sbjct: 301 LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ 360

Query: 368 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 427
           NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS
Sbjct: 361 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 420

Query: 428 QLEKALLKLNI 439
           QLEKALLKLNI
Sbjct: 421 QLEKALLKLNI 431

BLAST of CmaCh15G013140 vs. ExPASy TrEMBL
Match: A0A5A7TIP4 (Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001500 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.9e-206
Identity = 366/405 (90.37%), Postives = 384/405 (94.81%), Query Frame = 0

Query: 32  PEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYS 91
           P  + DPAA+LA  RPEFGEHGGVN+SIENSI+FTVMEAETMR+MFEGELGPE+D FIYS
Sbjct: 36  PMGKVDPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95

Query: 92  RHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHAL 151
           RHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLLQL+SSGGHIVASRTLYGGTHAL
Sbjct: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155

Query: 152 LSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHE 211
           LSHFLPRV  ITT+FVDV++H MVRDAMV+GKTKVLYFETVANPTLTVANIPELSRMAHE
Sbjct: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHE 215

Query: 212 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQ 271
           KGVTVVVDNTFSPMIISP RLGADVVVHSCSKFISGGADIIAGAVCG K LVNSMMDLHQ
Sbjct: 216 KGVTVVVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQ 275

Query: 272 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPD 331
           GTLMLLGPTMNAKVASELSQR+PHL LRMKEHS+R LVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 276 GTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335

Query: 332 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGS 391
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFG +AVSLG+FETL+SCSGS
Sbjct: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395

Query: 392 STSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           STSSE+N EEKELAGISPGLIRMSV YVGTLEQKWSQLEKALLK+
Sbjct: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKM 440

BLAST of CmaCh15G013140 vs. ExPASy TrEMBL
Match: A0A1S3AU15 (methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1)

HSP 1 Score: 727.2 bits (1876), Expect = 3.9e-206
Identity = 366/405 (90.37%), Postives = 384/405 (94.81%), Query Frame = 0

Query: 32  PEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYS 91
           P  + DPAA+LA  RPEFGEHGGVN+SIENSI+FTVMEAETMR+MFEGELGPE+D FIYS
Sbjct: 36  PMGKVDPAAALAMARPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95

Query: 92  RHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHAL 151
           RHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLLQL+SSGGHIVASRTLYGGTHAL
Sbjct: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155

Query: 152 LSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHE 211
           LSHFLPRV  ITT+FVDV++H MVRDAMV+GKTKVLYFETVANPTLTVANIPELSRMAHE
Sbjct: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHE 215

Query: 212 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQ 271
           KGVTVVVDNTFSPMIISP RLGADVVVHSCSKFISGGADIIAGAVCG K LVNSMMDLHQ
Sbjct: 216 KGVTVVVDNTFSPMIISPARLGADVVVHSCSKFISGGADIIAGAVCGSKDLVNSMMDLHQ 275

Query: 272 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPD 331
           GTLMLLGPTMNAKVASELSQR+PHL LRMKEHS+R LVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 276 GTLMLLGPTMNAKVASELSQRIPHLGLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335

Query: 332 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGS 391
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFG +AVSLG+FETL+SCSGS
Sbjct: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395

Query: 392 STSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           STSSE+N EEKELAGISPGLIRMSV YVGTLEQKWSQLEKALLK+
Sbjct: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWSQLEKALLKM 440

BLAST of CmaCh15G013140 vs. ExPASy TrEMBL
Match: A0A0A0KQL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 5.2e-206
Identity = 365/407 (89.68%), Postives = 387/407 (95.09%), Query Frame = 0

Query: 32  PEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYS 91
           P  E DPAA+LA   PEFGEHGGVN+SIENSI+FTVMEAETMR+MFEGELGPE+D FIYS
Sbjct: 36  PMGELDPAAALAMASPEFGEHGGVNMSIENSITFTVMEAETMRRMFEGELGPERDFFIYS 95

Query: 92  RHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHAL 151
           RHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLLQL+SSGGHIVASRTLYGGTHAL
Sbjct: 96  RHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLLQLVSSGGHIVASRTLYGGTHAL 155

Query: 152 LSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHE 211
           LSHFLPRV  ITT+FVDV++H MVRDAMV+GKTKVLYFETVANPTLTVANIPELSRMAH+
Sbjct: 156 LSHFLPRVSKITTTFVDVRNHEMVRDAMVEGKTKVLYFETVANPTLTVANIPELSRMAHQ 215

Query: 212 KGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQ 271
           KGVTVVVDNTFSP+IISPTRLGADVVVHSCSKFISGGADIIAGAVCG K+LVNSMMDLHQ
Sbjct: 216 KGVTVVVDNTFSPIIISPTRLGADVVVHSCSKFISGGADIIAGAVCGSKELVNSMMDLHQ 275

Query: 272 GTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPD 331
           GTLMLLGPTMNAKVASELSQR+PHL+LRMKEHS+R LVFAQRMKNLGLKVIYPGL DHPD
Sbjct: 276 GTLMLLGPTMNAKVASELSQRIPHLSLRMKEHSNRALVFAQRMKNLGLKVIYPGLNDHPD 335

Query: 332 HHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGS 391
           H LL SIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFG +AVSLG+FETL+SCSGS
Sbjct: 336 HQLLGSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGLMAVSLGFFETLMSCSGS 395

Query: 392 STSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKLNI 439
           STSSE+N EEKELAGISPGLIRMSV YVGTLEQKW+QLEKALLK+ I
Sbjct: 396 STSSELNGEEKELAGISPGLIRMSVGYVGTLEQKWNQLEKALLKMKI 442

BLAST of CmaCh15G013140 vs. ExPASy TrEMBL
Match: A0A6J1E0P6 (methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1)

HSP 1 Score: 698.7 bits (1802), Expect = 1.5e-197
Identity = 352/431 (81.67%), Postives = 385/431 (89.33%), Query Frame = 0

Query: 10  WDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVME 69
           W+ K K +K      P P T +   EEDPAA+LA    EFGEHGGVN+S+E SI+FTVME
Sbjct: 14  WNEKKKLIKYEKAKSPKPAT-KSATEEDPAAALALAEHEFGEHGGVNMSVEASITFTVME 73

Query: 70  AETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLL 129
           AETMR+MFEGE GPE +CFIYSRHFNPTVMAL RQMAALEGTEAAYCTSSGMAAVSSVLL
Sbjct: 74  AETMRRMFEGEEGPEGECFIYSRHFNPTVMALSRQMAALEGTEAAYCTSSGMAAVSSVLL 133

Query: 130 QLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYF 189
           QL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVD+KDH MV +AM++GKTKVLYF
Sbjct: 134 QLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDIKDHGMVANAMLEGKTKVLYF 193

Query: 190 ETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGA 249
           ETVANPTLTVA+IPELSRMAHEKGVTVVVDNTF+PM+ISP RLGADVVVHS SKFISGGA
Sbjct: 194 ETVANPTLTVADIPELSRMAHEKGVTVVVDNTFAPMVISPVRLGADVVVHSSSKFISGGA 253

Query: 250 DIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLV 309
           DIIAGAVCG KKLV+SMMDL QGTLMLLGPTMN KVA ELSQRVPHL LRMKEH HR L+
Sbjct: 254 DIIAGAVCGSKKLVHSMMDLQQGTLMLLGPTMNPKVACELSQRVPHLGLRMKEHCHRALI 313

Query: 310 FAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKD--YGYGGVLCIDMGTEERANRLMNLLQ 369
           FAQR++NLG KVIYPGLKDHPDHHL++SIAN    YGYGGVLCIDM TEERANRLMNLLQ
Sbjct: 314 FAQRIQNLGFKVIYPGLKDHPDHHLINSIANTSYGYGYGGVLCIDMETEERANRLMNLLQ 373

Query: 370 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 429
           NHT FG +AVSLG+FETL+SCSGSSTSSE+N EEKELAGISPGL+R+SV YVGTLEQKWS
Sbjct: 374 NHTKFGLMAVSLGFFETLMSCSGSSTSSELNAEEKELAGISPGLVRISVGYVGTLEQKWS 433

Query: 430 QLEKALLKLNI 439
           QLE+ LLK+ +
Sbjct: 434 QLERGLLKMKL 443

BLAST of CmaCh15G013140 vs. NCBI nr
Match: XP_022970043.1 (methionine gamma-lyase-like [Cucurbita maxima])

HSP 1 Score: 853.6 bits (2204), Expect = 7.5e-244
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 8   MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV 67
           MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV
Sbjct: 1   MLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTV 60

Query: 68  MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV 127
           MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV
Sbjct: 61  MEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSV 120

Query: 128 LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL 187
           LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL
Sbjct: 121 LLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVL 180

Query: 188 YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG 247
           YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG
Sbjct: 181 YFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISG 240

Query: 248 GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT 307
           GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT
Sbjct: 241 GADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRT 300

Query: 308 LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ 367
           LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ
Sbjct: 301 LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQ 360

Query: 368 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 427
           NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS
Sbjct: 361 NHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWS 420

Query: 428 QLEKALLKLNI 439
           QLEKALLKLNI
Sbjct: 421 QLEKALLKLNI 431

BLAST of CmaCh15G013140 vs. NCBI nr
Match: KAG7017043.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 820.5 bits (2118), Expect = 7.1e-234
Identity = 415/436 (95.18%), Postives = 422/436 (96.79%), Query Frame = 0

Query: 3   MAEVKMLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENS 62
           MAEVKM WDVKNK+LK TA  PP PVTWRPE EEDPAASLAA RPEFGEHGGVNLSIENS
Sbjct: 1   MAEVKMWWDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENS 60

Query: 63  ISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMA 122
           I+FTVMEAETMRKMFEGELGPEQD FIYSRHFNPTVMALGRQMAA+EGTEAAYCTSSGMA
Sbjct: 61  ITFTVMEAETMRKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAAIEGTEAAYCTSSGMA 120

Query: 123 AVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKG 182
           A+SSVLLQL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHA VRDAMVK 
Sbjct: 121 AISSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKD 180

Query: 183 KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 242
           KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS
Sbjct: 181 KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 240

Query: 243 KFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 302
           KFISGGADIIAGAVCG KKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE
Sbjct: 241 KFISGGADIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 300

Query: 303 HSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 362
           HSHR LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL
Sbjct: 301 HSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 360

Query: 363 MNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTL 422
           MNLLQNHTHFG LAVSLGYFETL+SCSGSSTSSEMNVEEKELAGISPGLIRMSV YVGTL
Sbjct: 361 MNLLQNHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTL 420

Query: 423 EQKWSQLEKALLKLNI 439
           EQKWSQLEKALLK+NI
Sbjct: 421 EQKWSQLEKALLKMNI 436

BLAST of CmaCh15G013140 vs. NCBI nr
Match: KAG6579584.1 (Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815.8 bits (2106), Expect = 1.7e-232
Identity = 414/436 (94.95%), Postives = 420/436 (96.33%), Query Frame = 0

Query: 3   MAEVKMLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENS 62
           MAEVKM WDVKNK+LK TA  PP PVTWRPE EEDPAASLAA RPEFGEHGGVNLSIENS
Sbjct: 1   MAEVKMWWDVKNKRLKHTAPPPPPPVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENS 60

Query: 63  ISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMA 122
           I+FTVMEAETM KMFEGELGPEQD FIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMA
Sbjct: 61  ITFTVMEAETMSKMFEGELGPEQDFFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMA 120

Query: 123 AVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKG 182
           A+SSVLLQL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHA VRDAMVK 
Sbjct: 121 AISSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAKVRDAMVKD 180

Query: 183 KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 242
           KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS
Sbjct: 181 KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 240

Query: 243 KFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 302
           KFISG ADIIAGAVCG KKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE
Sbjct: 241 KFISGRADIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 300

Query: 303 HSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 362
           HSHR LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL
Sbjct: 301 HSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 360

Query: 363 MNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTL 422
           MNLLQNHTHFG LAVSLGYFETL+SCSGSSTSSEMNVEEKELAGISPGLIRMSV YVGTL
Sbjct: 361 MNLLQNHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTL 420

Query: 423 EQKWSQLEKALLKLNI 439
           EQKWSQLEKALLK+NI
Sbjct: 421 EQKWSQLEKALLKMNI 436

BLAST of CmaCh15G013140 vs. NCBI nr
Match: XP_023520565.1 (methionine gamma-lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 813.9 bits (2101), Expect = 6.6e-232
Identity = 416/436 (95.41%), Postives = 420/436 (96.33%), Query Frame = 0

Query: 3   MAEVKMLWDVKNKKLKPTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENS 62
           MAEVKM WD KNKKLKPTA  PP  VTWRPE EEDPAASLAA RPEFGEHGGVNLSIENS
Sbjct: 1   MAEVKMWWD-KNKKLKPTAPPPPPTVTWRPEGEEDPAASLAAARPEFGEHGGVNLSIENS 60

Query: 63  ISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMA 122
           I+FTVMEAE MRKMFEGELG EQD FIYSRHFNPTVMALGR MAALEGTEAAYCTSSGMA
Sbjct: 61  ITFTVMEAEMMRKMFEGELGLEQDFFIYSRHFNPTVMALGRAMAALEGTEAAYCTSSGMA 120

Query: 123 AVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKG 182
           AVSSVLLQL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKG
Sbjct: 121 AVSSVLLQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKG 180

Query: 183 KTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 242
           KTKVLYFETVANPTLTVAN+PELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS
Sbjct: 181 KTKVLYFETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCS 240

Query: 243 KFISGGADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 302
           KFISGGADIIAGAVCG KKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE
Sbjct: 241 KFISGGADIIAGAVCGSKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKE 300

Query: 303 HSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 362
           HSHR LVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL
Sbjct: 301 HSHRALVFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRL 360

Query: 363 MNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTL 422
           MNLLQNHTHFG LAVSLGYFETL+SCSGSSTSSEMNVEEKELAGISPGLIRMSV YVGTL
Sbjct: 361 MNLLQNHTHFGLLAVSLGYFETLMSCSGSSTSSEMNVEEKELAGISPGLIRMSVGYVGTL 420

Query: 423 EQKWSQLEKALLKLNI 439
           EQKWSQLEKALLKLNI
Sbjct: 421 EQKWSQLEKALLKLNI 435

BLAST of CmaCh15G013140 vs. NCBI nr
Match: XP_038875936.1 (methionine gamma-lyase-like [Benincasa hispida])

HSP 1 Score: 745.7 bits (1924), Expect = 2.2e-211
Identity = 375/430 (87.21%), Postives = 397/430 (92.33%), Query Frame = 0

Query: 10  WDVKNKKLK-PTARLPPLPVTWRPEEEEDPAASLAAVRPEFGEHGGVNLSIENSISFTVM 69
           W++ NKK    T    P+  +W P  +EDPAA+LA  RPEFGEHGGVN+SIENSI+FTVM
Sbjct: 15  WNIDNKKFSYQTKSATPMVTSW-PMGDEDPAATLAMARPEFGEHGGVNMSIENSITFTVM 74

Query: 70  EAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVL 129
           EAETMR MFEGELGPE+D FIYSRHFNPTV+AL RQMAALEGTEAAYCTSSGMAAVSSVL
Sbjct: 75  EAETMRMMFEGELGPERDFFIYSRHFNPTVLALSRQMAALEGTEAAYCTSSGMAAVSSVL 134

Query: 130 LQLLSSGGHIVASRTLYGGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLY 189
           LQL+SSGGHIVASRTLYGGTHALLSHFLPRVCNITT+FVDVKDH MVRDAMV+GKTKVLY
Sbjct: 135 LQLVSSGGHIVASRTLYGGTHALLSHFLPRVCNITTTFVDVKDHEMVRDAMVEGKTKVLY 194

Query: 190 FETVANPTLTVANIPELSRMAHEKGVTVVVDNTFSPMIISPTRLGADVVVHSCSKFISGG 249
           FETVANPTLTVAN+PELSRMAHEKGVTVVVDNTFSPMIISP RLGADVVVHSCSKFISGG
Sbjct: 195 FETVANPTLTVANVPELSRMAHEKGVTVVVDNTFSPMIISPARLGADVVVHSCSKFISGG 254

Query: 250 ADIIAGAVCGKKKLVNSMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTL 309
           ADIIAGAVCG K LVNSMMDLHQGTLMLLGPTMNAKVASE+SQR+PHL LRMKEHSHR L
Sbjct: 255 ADIIAGAVCGSKNLVNSMMDLHQGTLMLLGPTMNAKVASEISQRIPHLGLRMKEHSHRAL 314

Query: 310 VFAQRMKNLGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCIDMGTEERANRLMNLLQN 369
           VFAQRMKNLGLKV YPGL DHPDH LLS IANKDYGYGGVLCIDMGTEERANRLMNLLQN
Sbjct: 315 VFAQRMKNLGLKVTYPGLNDHPDHQLLSYIANKDYGYGGVLCIDMGTEERANRLMNLLQN 374

Query: 370 HTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQ 429
           HTHFG +AVSLG+FETL+SCSGSSTSSE+N +EKELAGISPGLIRMSV YVGTLEQKWSQ
Sbjct: 375 HTHFGLMAVSLGFFETLMSCSGSSTSSELNSKEKELAGISPGLIRMSVGYVGTLEQKWSQ 434

Query: 430 LEKALLKLNI 439
           LEKALLK+ I
Sbjct: 435 LEKALLKMKI 443

BLAST of CmaCh15G013140 vs. TAIR 10
Match: AT1G64660.1 (methionine gamma-lyase )

HSP 1 Score: 617.5 bits (1591), Expect = 8.5e-177
Identity = 297/402 (73.88%), Postives = 353/402 (87.81%), Query Frame = 0

Query: 35  EEDPAASLAAVRPEFGEHGGVNLSIENSISFTVMEAETMRKMFEGELGPEQDCFIYSRHF 94
           + DPAA++A +R EFGEHGGVN+SIE S +FTVME +TMR+MF GELGP+ D ++YSRHF
Sbjct: 40  DADPAAAIANIRHEFGEHGGVNMSIEASATFTVMEPDTMRRMFTGELGPDNDFYVYSRHF 99

Query: 95  NPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSH 154
           NPTV+ L RQMAALEGT+AAYCTSSGM+A+SSV+LQL SSGGH+VA+ TLYGGTHALLSH
Sbjct: 100 NPTVLNLSRQMAALEGTQAAYCTSSGMSAISSVMLQLCSSGGHVVAASTLYGGTHALLSH 159

Query: 155 FLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGV 214
           FLPR CNITTSFVD+ DH  V +A+V+G+T+VLYFE+VANPTLTVA+IPELSRMAHEKGV
Sbjct: 160 FLPRTCNITTSFVDITDHGAVANAIVEGRTQVLYFESVANPTLTVADIPELSRMAHEKGV 219

Query: 215 TVVVDNTFSPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSMMDLHQGTL 274
           TVVVDNTF+PM++SP +LGADVVVHS SKFISGGADIIAGAVCG + LV  MMDL  G+L
Sbjct: 220 TVVVDNTFAPMVLSPAKLGADVVVHSISKFISGGADIIAGAVCGSENLVKEMMDLRGGSL 279

Query: 275 MLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMKNLGLKVIYPGLKDHPDHHL 334
           MLLGPTMNAKVA ELS+R+PHL LRM+EHSHR  V+A+RM++LG+KVIYPGL+ HP H L
Sbjct: 280 MLLGPTMNAKVAFELSERIPHLGLRMREHSHRAQVYAERMRDLGMKVIYPGLETHPQHKL 339

Query: 335 LSSIANKDYGYGGVLCIDMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTS 394
              + N+DYGYGG+L IDM TEE+AN+LM  LQN T FGF+AVSLGY+ETL+SCSGSSTS
Sbjct: 340 FKGMVNRDYGYGGLLSIDMETEEKANKLMAYLQNATQFGFMAVSLGYYETLMSCSGSSTS 399

Query: 395 SEMNVEEKELAGISPGLIRMSVAYVGTLEQKWSQLEKALLKL 437
           SE++  +KE AGISPGL+RMSV YVGTLEQKW+Q EKA L++
Sbjct: 400 SELDPSQKEAAGISPGLVRMSVGYVGTLEQKWTQFEKAFLRM 441

BLAST of CmaCh15G013140 vs. TAIR 10
Match: AT3G01120.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.7e-39
Identity = 106/332 (31.93%), Postives = 180/332 (54.22%), Query Frame = 0

Query: 88  FIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLYGG 147
           F Y R+ NPT + L  +++ALEG E+    +SGM A + +LL L+ +GGHIV +   Y  
Sbjct: 224 FEYGRYGNPTTVVLEDKISALEGAESTLVMASGMCASTVMLLALVPAGGHIVTTTDCYRK 283

Query: 148 THALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPELSR 207
           T   + +FLP++  IT + +D  D A +  A+ + K  + + E+  NP L   +I  +S+
Sbjct: 284 TRIFMENFLPKL-GITVTVIDPADIAGLEAAVNEFKVSLFFTESPTNPFLRCVDIELVSK 343

Query: 208 MAHEKGVTVVVDNTF-SPMIISPTRLGADVVVHSCSKFISGGADIIAGAVCGKKKLVNSM 267
           + H++G  V +D TF +P+      LGAD+VVHS +K+I G  D++AG +CG  KLV+ +
Sbjct: 344 ICHKRGTLVCIDGTFATPLNQKALALGADLVVHSATKYIGGHNDVLAGCICGSLKLVSEI 403

Query: 268 MDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMK--NLGLKVIYP 327
            +LH     +LG T+N   A  + + +  L LR+++ +      A+ ++       V YP
Sbjct: 404 RNLHH----VLGGTLNPNAAYLIIRGMKTLHLRVQQQNSTAFRMAEILEAHPKVSHVYYP 463

Query: 328 GLKDHPDHHLLSSIANKDYGYGGVLCIDM-GTEERANRLMNLLQNHTHFGFLAVSLGYFE 387
           GL  HP+H L      +  G+GGV+  ++ G  E   + ++ L+      ++A S G  E
Sbjct: 464 GLPSHPEHELAK---RQMTGFGGVVSFEIDGDIETTIKFVDSLK----IPYIAPSFGGCE 523

Query: 388 TLISCSGSSTSSEMNVEEKELAGISPGLIRMS 416
           +++      +  ++  EE+   GI   L+R S
Sbjct: 524 SIVDQPAIMSYWDLPQEERLKYGIKDNLVRFS 543

BLAST of CmaCh15G013140 vs. TAIR 10
Match: AT3G57050.1 (cystathionine beta-lyase )

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-34
Identity = 116/364 (31.87%), Postives = 188/364 (51.65%), Query Frame = 0

Query: 61  NSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSG 120
           +++S  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C +SG
Sbjct: 102 DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 161

Query: 121 MAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPR----VCNITTSFVDVKDHAMVR 180
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR    V  + T+ +D        
Sbjct: 162 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEV------ 221

Query: 181 DAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNT-FSPMIISPTRLGAD 240
            A +  +TK+++ E+  NP   +++I ++S MAH +G  V+VDN+  SP++  P  LGAD
Sbjct: 222 AAAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGAD 281

Query: 241 VVVHSCSKFISGGADIIAGAVCGK-KKLVNSMMDLHQGTLMLLGPTMNAKVASELSQR-V 300
           +V+HS +KFI+G +D++AG +  K +KL   +  L       L P         L  R +
Sbjct: 282 IVMHSATKFIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAP-----FDCWLCLRGI 341

Query: 301 PHLALRMKEHSHRTLVFAQRMKN--LGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCI 360
             +ALR+++        A  + +     KV Y GL DHP HHL  S A    G G V   
Sbjct: 342 KTMALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFSF 401

Query: 361 DMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGL 416
             G+   +  L+      T +  +AVS G  ++LIS     + + +  E +E  G++  L
Sbjct: 402 ITGSVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDL 442

BLAST of CmaCh15G013140 vs. TAIR 10
Match: AT3G57050.2 (cystathionine beta-lyase )

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-34
Identity = 116/364 (31.87%), Postives = 188/364 (51.65%), Query Frame = 0

Query: 61  NSISFTVMEAETMRKMFEGELGPEQDCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSG 120
           +++S  + +  T ++    E GP    + Y+R  NPT  AL   +A L+  + A+C +SG
Sbjct: 87  DAMSTPLYQTATFKQPSAIENGP----YDYTRSGNPTRDALESLLAKLDKADRAFCFTSG 146

Query: 121 MAAVSSVLLQLLSSGGHIVASRTLYGGTHALLSHFLPR----VCNITTSFVDVKDHAMVR 180
           MAA+S+V   L+ +G  IVA   +YGG+  LLS  +PR    V  + T+ +D        
Sbjct: 147 MAALSAV-THLIKNGEEIVAGDDVYGGSDRLLSQVVPRSGVVVKRVNTTKLDEV------ 206

Query: 181 DAMVKGKTKVLYFETVANPTLTVANIPELSRMAHEKGVTVVVDNT-FSPMIISPTRLGAD 240
            A +  +TK+++ E+  NP   +++I ++S MAH +G  V+VDN+  SP++  P  LGAD
Sbjct: 207 AAAIGPQTKLVWLESPTNPRQQISDIRKISEMAHAQGALVLVDNSIMSPVLSRPLELGAD 266

Query: 241 VVVHSCSKFISGGADIIAGAVCGK-KKLVNSMMDLHQGTLMLLGPTMNAKVASELSQR-V 300
           +V+HS +KFI+G +D++AG +  K +KL   +  L       L P         L  R +
Sbjct: 267 IVMHSATKFIAGHSDVMAGVLAVKGEKLAKEVYFLQNSEGSGLAP-----FDCWLCLRGI 326

Query: 301 PHLALRMKEHSHRTLVFAQRMKN--LGLKVIYPGLKDHPDHHLLSSIANKDYGYGGVLCI 360
             +ALR+++        A  + +     KV Y GL DHP HHL  S A    G G V   
Sbjct: 327 KTMALRIEKQQENARKIAMYLSSHPRVKKVYYAGLPDHPGHHLHFSQAK---GAGSVFSF 386

Query: 361 DMGTEERANRLMNLLQNHTHFGFLAVSLGYFETLISCSGSSTSSEMNVEEKELAGISPGL 416
             G+   +  L+      T +  +AVS G  ++LIS     + + +  E +E  G++  L
Sbjct: 387 ITGSVALSKHLVET----TKYFSIAVSFGSVKSLISMPCFMSHASIPAEVREARGLTEDL 427

BLAST of CmaCh15G013140 vs. TAIR 10
Match: AT1G33320.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 1.0e-33
Identity = 101/334 (30.24%), Postives = 178/334 (53.29%), Query Frame = 0

Query: 86  DCFIYSRHFNPTVMALGRQMAALEGTEAAYCTSSGMAAVSSVLLQLLSSGGHIVASRTLY 145
           D + Y+R+ NPT MAL  +++ LEG E+    +SGM A + +LL L+ + GHIVA++  Y
Sbjct: 72  DLYEYARYGNPTTMALEEKISVLEGAESTLVMASGMYASNVMLLALVPTNGHIVATKDCY 131

Query: 146 GGTHALLSHFLPRVCNITTSFVDVKDHAMVRDAMVKGKTKVLYFETVANPTLTVANIPEL 205
             T   + +FL ++  IT +F+D  D A ++  +   +  + + E+  NP L   +I  +
Sbjct: 132 KETRIFMENFLTKL-GITVTFIDSDDIAGLQTLVNNHEVSLFFTESPTNPFLRCVDIKLV 191

Query: 206 SRMAHEKGVTVVVDNTFSPMIISPT-RLGADVVVHSCSKFISGGADIIAGAVCGKKKLVN 265
           S++ H +G  V +D T +  I   T  LGAD+V HS +K+I G  D +AG++ G  +LV+
Sbjct: 192 SKICHRRGTLVCIDATIATPINQKTLALGADLVHHSATKYIGGHNDFLAGSISGSMELVS 251

Query: 266 SMMDLHQGTLMLLGPTMNAKVASELSQRVPHLALRMKEHSHRTLVFAQRMK--NLGLKVI 325
            + +LH+    LLG T+N   A  L + +  + LR+++ +   +  AQ ++      +V 
Sbjct: 252 KIRNLHK----LLGGTLNPNAAYLLIRGMKTMHLRVRQQNSTGMKMAQVLEAHPKVSRVY 311

Query: 326 YPGLKDHPDHHLLSSIANKDYGYGGVLCIDM-GTEERANRLMNLLQNHTHFGFLAVSLGY 385
           Y GL  HP+H +      +  G GG++  ++ G  +   + ++ L+      +LA S G 
Sbjct: 312 YLGLPSHPEHLIAK---RQMTGIGGLISFEIDGDLKTTIKFIDALK----IPYLAASFGG 371

Query: 386 FETLISCSGSSTSSEMNVEEKELAGISPGLIRMS 416
            E+L+    +    ++  EE+   G    L+R S
Sbjct: 372 CESLVDQLATGI-WDIPREERLKDGFQDNLVRFS 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SGU91.2e-17573.88Methionine gamma-lyase OS=Arabidopsis thaliana OX=3702 GN=MGL PE=1 SV=1[more]
Q8L0X45.0e-4936.99L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. polymorphum OX=76857 ... [more]
Q8RDT41.6e-4736.06L-methionine gamma-lyase OS=Fusobacterium nucleatum subsp. nucleatum (strain ATC... [more]
Q7MX711.7e-4436.54L-methionine gamma-lyase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83)... [more]
Q73KL73.2e-4333.50L-methionine gamma-lyase OS=Treponema denticola (strain ATCC 35405 / DSM 14222 /... [more]
Match NameE-valueIdentityDescription
A0A6J1I2P53.7e-244100.00methionine gamma-lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111469051 PE=3 SV=... [more]
A0A5A7TIP43.9e-20690.37Methionine gamma-lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3AU153.9e-20690.37methionine gamma-lyase-like OS=Cucumis melo OX=3656 GN=LOC103482860 PE=3 SV=1[more]
A0A0A0KQL75.2e-20689.68Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G148520 PE=3 SV=1[more]
A0A6J1E0P61.5e-19781.67methionine gamma-lyase OS=Momordica charantia OX=3673 GN=LOC111026086 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022970043.17.5e-244100.00methionine gamma-lyase-like [Cucurbita maxima][more]
KAG7017043.17.1e-23495.18Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579584.11.7e-23294.95Methionine gamma-lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023520565.16.6e-23295.41methionine gamma-lyase-like [Cucurbita pepo subsp. pepo][more]
XP_038875936.12.2e-21187.21methionine gamma-lyase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G64660.18.5e-17773.88methionine gamma-lyase [more]
AT3G01120.11.7e-3931.93Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
AT3G57050.12.1e-3431.87cystathionine beta-lyase [more]
AT3G57050.22.1e-3431.87cystathionine beta-lyase [more]
AT1G33320.11.0e-3330.24Pyridoxal phosphate (PLP)-dependent transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePFAMPF01053Cys_Met_Meta_PPcoord: 51..416
e-value: 2.8E-81
score: 273.0
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePIRSFPIRSF001434CGScoord: 30..437
e-value: 3.8E-98
score: 326.7
IPR000277Cys/Met metabolism, pyridoxal phosphate-dependent enzymePANTHERPTHR11808TRANS-SULFURATION ENZYME FAMILY MEMBERcoord: 36..435
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 40..293
e-value: 5.1E-67
score: 227.8
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 297..437
e-value: 4.5E-23
score: 83.9
NoneNo IPR availablePIRSRPIRSR001434-2PIRSR001434-2coord: 91..397
e-value: 2.4E-78
score: 261.2
NoneNo IPR availablePANTHERPTHR11808:SF76METHIONINE GAMMA-LYASE-LIKEcoord: 36..435
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 52..432

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G013140.1CmaCh15G013140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1901566 organonitrogen compound biosynthetic process
biological_process GO:0019346 transsulfuration
molecular_function GO:0018826 methionine gamma-lyase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity