CmaCh15G012810 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh15G012810
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionUBR-type domain-containing protein
LocationCma_Chr15: 8118914 .. 8122409 (-)
RNA-Seq ExpressionCmaCh15G012810
SyntenyCmaCh15G012810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGTTAGTTGAGTTACTTCCTCTAAATTCCAAAACCCAAAGACTTGGAAGTGGAGGGAACCATCAGCGAACTATATATTTGATTTCAGCGATGAAAGAGCCCACTTTTGCTAATTGTGGCGGGAGAATGCCGTAAGAGTCCCTGCTCATCCAAACCCAATTCCATTTTTTATCTATCAACGAGGGGAAAATCCTTTCTCAATCGACGCTACTTGTGTTGTGCTTCAATTTGATCGCCTTCAGGTTCTGGAGCGGTCTCTCAACTCCAAATGAGGTACTTTTTTTCTCTTCAAGTTTGGTGGGATTTCGCGCTTTTGTTCTAGGGCATCTCCCTATGCTGTTTTTTTTCCTTTCGAATTTTCATTATGGGGTTTTGAGTGTAATTAATGTTTTGTTTGGTCTTCAGGTTTGGATCCTGAGAACTGGTTTCGATTATCAATAGTGGAGTGTTATTGAAATTACTTAATCGTTTGATTTGAAATGGCGGAAGTTTTCGACGACGAAACCGAACAAACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGTAACTCTCTCTCTTCTCCTCTTTCAATTTCGATGTCTGAGTATTGGTTGGTTTTCCAAAATTTTAGCGACATAATGGCTTGGAAACGTACTGGGTTTTCTATAATTTAGGGTTCATGGCCGCAAAACTTTCAAAATTCTTAAAATCGATTGGAGCGTCCAATTTCTTGTAAGTCGATCTCCTGTCGCGACGTTTTGTGTGTGTCCGTAGTGTTAGTAGAGGATAGTCAGATCACCTGTATTGTTCTAAATTTTCCAATTAGCGGAGGTCATTTGGGCTTTCTACAGAGAATATGATACAAACTCTAATGTCTTCTCTTCTTGTTTCTGTATCTTCATTTTGGGTGCACGGTATCTGATTATTATTATTATTATCATTTTATTGATCAAGGAAGCGGATTTAGTTTTGGGAGGCGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCCTGTAGCTTGTCATGCCATGATGGACATGAGGTATACATGTTCTTAGAATGAACTCTGTTTAGATATGCAGGGGTTTACGTGTTTTTTTTTTTGTCCAGTTTATGGTGGCAACTATTGATACCATGGCTCAGATTGCTTTTAAGCAGCATGATCCATATGATATAAAAGATGTACTTTTTTACCATATTTAACTGATCCCTTTTTTGACACCTGAGTATTTGTTGAAGATCCAGCTTGTTGTATGTGGTTCTGTAACAGCTCAAGCCCACTGCTAGCAGATATTGTCCTCTTTGTGTTTTCCCGTTTGGGCTTTTCCTCAAGGTTTTTAAAACGTGTATACTAGAGAGAGATTTCCACACCCTTATAAGGAATACTTCGTTACCCTCTCCAACCAATGTGGGACCTCACAATCTACCCCCTTGGGGGCCAGCGTCCTAGTATATCGCTCTGATACCATTTGTAACAGTCCAAGCACACCGCTAGTATATATTATCCTTTTTTAGCTTTCTCTTTTGGGCTCCTCTCAAGGTTTCTAAAATGCGTCTACTAGGGAGAGATTTTCACATTCTTATAAGGAATGCTTCGTTCCCCTCTCCAACGACGTGGGACCTCACAGGTTTTGCTTATGCGAAAACATTTAGGGTATTTGTCATGTTGTAAATTTAGGTTATTGATTCAGGAACACGTTTACTTGCCGATTGTCAATAATTATTTCGATGATGTTAAACTCTCCAAGTATTTGGCTTCTTAACGCCCCCGCCTCCGCCCCATTTTTATTGTGATCATTGTTCTCTTGGGCCAGATTGTGGAATTATGGACCAAAAGAAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATCTGCAAGCTGTTCTCGAACAAAGACGTAGAAAATGCCAACAACTCATATAATCATAATTTTAAAGGATCGTACTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGGTGTGTTAGCACAAAAGCCTTCCATGGGTTTTCAATTATTTTGTCTCGTTTCTCCATTTTTGTTGTTTTACAATGTTTTGTTGCTTATCATTAAAAGAGTCGAGAGATTTGATGAACCTTGAAATAACCTGGACTTCATTGTACTTGTTCGTTCTGCAGATTCCAAGAGACGAAGAAGGGGAGCCCCTATACGAGGACTTTATATGCCCAGCGTGTTCGGCAATTTGTTCTTTCCTGATTCAATATCCTTCATCTATTTGGGCTGTGGGAAGGCACTCTGCTGGAAAGGACAGCTTGCTTAGTGAACTTCCTGAGAATAATTCTGGTTCTAAGGAATCTGTTAAAGATAACACTCAAACTGATAGTTGTGTGCTTGAAACCTCTTCATCTGCTTCCATAACTGCTGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCATTGTGCAGATGTGAAAAATGCATCAATAATTACAAGCTGAAGAACATTAGTTTCTTGGTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGGAACAAGAGGGTGCCGAGTTAAAGCTGTTTGATAACCTGGGGCATGTGGAGAAGATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCGTACATTCATGGTATTCACTCATTATATGTTTGCTTTGCTTGTGTTCCTATTTTCCTTGGTTATATGATTTGTTCTACGTTGCAGGAATCGTTCGACTCGTCGAAGCCGATTACGTCTGCAGATATCCACCAGGTATTTGAAAATCTAGCTAAAAAGAGACGTCGTACTGAATGAAATTTCTTAGGAGCAGCAATAACTACTACAGCGGATGGTCTAGGATTTCTCAAGTAAGCAGTCCTGAGTCCTGGCTAATCGAATATACGCCGTGGGCTGGTTTCTTTAGACTGTTAACTATGAAACATAATTTACTTTGACATCTAGATTATGCTTTCAGAAATGGCTTGTTTTTTAAACCTTGTTGGCTACCGGAGGCGTCGAACTTTTTTTGTGACATGCTATGTTTTCTCCCCCGTCACTAGTAGGTTGATGGGTGAACCATCACCATGGTTTTAGCTGTAAATTTAACTTAGGTTCGGACTTGTTTCTAATATATGCTAGACCTATTTCCGACATAATGCTGTCCATTCTCTGTACATGTAGCCTCGTTTTCTTGTAGATATTACTTTGTCTTAGTTCGATAGATCTTCTCCACCGATAGATTGTTTATGTATTGTTGTATCTCAATTCAAATTTAGTCTTTAGTTAGTCAAGAAGGAAAATCTTTTGTTCTTCTAAATTGATCC

mRNA sequence

TTGTTAGTTGAGTTACTTCCTCTAAATTCCAAAACCCAAAGACTTGGAAGTGGAGGGAACCATCAGCGAACTATATATTTGATTTCAGCGATGAAAGAGCCCACTTTTGCTAATTGTGGCGGGAGAATGCCGTAAGAGTCCCTGCTCATCCAAACCCAATTCCATTTTTTATCTATCAACGAGGGGAAAATCCTTTCTCAATCGACGCTACTTGTGTTGTGCTTCAATTTGATCGCCTTCAGGTTCTGGAGCGGTCTCTCAACTCCAAATGAGGTTTGGATCCTGAGAACTGGTTTCGATTATCAATAGTGGAGTGTTATTGAAATTACTTAATCGTTTGATTTGAAATGGCGGAAGTTTTCGACGACGAAACCGAACAAACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCGGATTTAGTTTTGGGAGGCGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCCTGTAGCTTGTCATGCCATGATGGACATGAGATTGTGGAATTATGGACCAAAAGAAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATCTGCAAGCTGTTCTCGAACAAAGACGTAGAAAATGCCAACAACTCATATAATCATAATTTTAAAGGATCGTACTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGATTCCAAGAGACGAAGAAGGGGAGCCCCTATACGAGGACTTTATATGCCCAGCGTGTTCGGCAATTTGTTCTTTCCTGATTCAATATCCTTCATCTATTTGGGCTGTGGGAAGGCACTCTGCTGGAAAGGACAGCTTGCTTAGTGAACTTCCTGAGAATAATTCTGGTTCTAAGGAATCTGTTAAAGATAACACTCAAACTGATAGTTGTGTGCTTGAAACCTCTTCATCTGCTTCCATAACTGCTGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCATTGTGCAGATGTGAAAAATGCATCAATAATTACAAGCTGAAGAACATTAGTTTCTTGGTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGGAACAAGAGGGTGCCGAGTTAAAGCTGTTTGATAACCTGGGGCATGTGGAGAAGATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCGTACATTCATGGAATCGTTCGACTCGTCGAAGCCGATTACGTCTGCAGATATCCACCAGGTATTTGAAAATCTAGCTAAAAAGAGACGTCGTACTGAATGAAATTTCTTAGGAGCAGCAATAACTACTACAGCGGATGGTCTAGGATTTCTCAAGTAAGCAGTCCTGAGTCCTGGCTAATCGAATATACGCCGTGGGCTGGTTTCTTTAGACTGTTAACTATGAAACATAATTTACTTTGACATCTAGATTATGCTTTCAGAAATGGCTTGTTTTTTAAACCTTGTTGGCTACCGGAGGCGTCGAACTTTTTTTGTGACATGCTATGTTTTCTCCCCCGTCACTAGTAGGTTGATGGGTGAACCATCACCATGGTTTTAGCTGTAAATTTAACTTAGGTTCGGACTTGTTTCTAATATATGCTAGACCTATTTCCGACATAATGCTGTCCATTCTCTGTACATGTAGCCTCGTTTTCTTGTAGATATTACTTTGTCTTAGTTCGATAGATCTTCTCCACCGATAGATTGTTTATGTATTGTTGTATCTCAATTCAAATTTAGTCTTTAGTTAGTCAAGAAGGAAAATCTTTTGTTCTTCTAAATTGATCC

Coding sequence (CDS)

ATGGCGGAAGTTTTCGACGACGAAACCGAACAAACGATTTCTATTCAGGAGTATCTGAAGGAAGTTGAGGAACAGGAGCTGGAAGCGGATTTAGTTTTGGGAGGCGACGAAGGAAAGGAATGTACTTACAGCAAGGGTTATATGAAGAGACAAGCCATTTTCTCATGTCTAACTTGTACCCCTGATGGAAATGCTGGAGTTTGCACAGCCTGTAGCTTGTCATGCCATGATGGACATGAGATTGTGGAATTATGGACCAAAAGAAACTTCAGGTGCGACTGTGGAAATTCAAAATTTGGAGATTTCATCTGCAAGCTGTTCTCGAACAAAGACGTAGAAAATGCCAACAACTCATATAATCATAATTTTAAAGGATCGTACTGCATATGCAATCGCCCTTATCCTGATCCAGATGTTGAAGAGCAGGTGGAGATGATTCAGTGCTGTATGTGTGAGGATTGGTTCCATGAGGAACATCTTGGTCTCAGTTCCATAGATGAGATTCCAAGAGACGAAGAAGGGGAGCCCCTATACGAGGACTTTATATGCCCAGCGTGTTCGGCAATTTGTTCTTTCCTGATTCAATATCCTTCATCTATTTGGGCTGTGGGAAGGCACTCTGCTGGAAAGGACAGCTTGCTTAGTGAACTTCCTGAGAATAATTCTGGTTCTAAGGAATCTGTTAAAGATAACACTCAAACTGATAGTTGTGTGCTTGAAACCTCTTCATCTGCTTCCATAACTGCTGAAAACAAGCCAATGTTTCTTTCAAAGAACTGGAGGGGTGCATTGTGCAGATGTGAAAAATGCATCAATAATTACAAGCTGAAGAACATTAGTTTCTTGGTAGATGAAGAGGACTCCATTGCTGAGTATGAGAAAATAGCAAAGCAGAAGAGGGAAGAGAAGTTACAGGAACAAGAGGGTGCCGAGTTAAAGCTGTTTGATAACCTGGGGCATGTGGAGAAGATTGAGATTCTAAATGGCATTGCAGATATGAAGGATGAGATTCGTACATTCATGGAATCGTTCGACTCGTCGAAGCCGATTACGTCTGCAGATATCCACCAGGTATTTGAAAATCTAGCTAAAAAGAGACGTCGTACTGAATGA

Protein sequence

MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYEDFICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFENLAKKRRRTE
Homology
BLAST of CmaCh15G012810 vs. ExPASy Swiss-Prot
Match: Q8BU04 (Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.2e-65
Identity = 152/415 (36.63%), Postives = 218/415 (52.53%), Query Frame = 0

Query: 8   ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGN--A 67
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G + RQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVGRQALYACSTCTPEGEEPA 72

Query: 68  GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFKG 127
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKLF +K   N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLFPDKSKVNSCNKYNDNFFG 132

Query: 128 SYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYEDFICPA 187
            YC+C RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G+  +++ +C A
Sbjct: 133 LYCVCKRPYPDPEDEVPDEMIQCVVCEDWFHGRHLG-----AIP-PESGD--FQEMVCQA 192

Query: 188 CSAICSFLIQYPSSIWAVGRHSAGKDSLLSEL----------PEN--------------- 247
           C   CSFL  Y + + AV R SA  D LL             PEN               
Sbjct: 193 CMRRCSFLWAYAAQL-AVTRISAEDDGLLPNATGMGDEDVSKPENGAPQDNGLKEDAPEH 252

Query: 248 -----------------NSGSKES------VKDNTQTD---SCVLETSSSASITAENKPM 307
                            +S S ES       K+N +T+   SC L+   +     ++   
Sbjct: 253 GRDSVNEVKAEQKNEPCSSSSSESDLQTVFKKENIKTEPQSSCRLQELQAKQFVKKDAAT 312

Query: 308 FLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELKL 367
           +   NWR  LC C+ C+  Y   ++ FL DE D++  YE   K    ++  ++    +  
Sbjct: 313 YWPLNWRSKLCTCQDCMKMYGELDVLFLTDECDTVLAYENKGK---NDQATDRRDPLMDT 372

Query: 368 FDNLGHVEKIEILNGIADMKDEIRTFMESF-DSSKPITSADIHQVFENLAKKRRR 369
             ++  V+++E++    D+K E++ +++ F D    +   DI Q FE    K+RR
Sbjct: 373 LSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRR 415

BLAST of CmaCh15G012810 vs. ExPASy Swiss-Prot
Match: Q8N806 (Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 SV=2)

HSP 1 Score: 245.0 bits (624), Expect = 1.4e-63
Identity = 148/415 (35.66%), Postives = 213/415 (51.33%), Query Frame = 0

Query: 8   ETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGN--A 67
           E E  +S+ + L+E EE E EA  VLGG + ++C+YS+G +KRQA+++C TCTP+G   A
Sbjct: 13  ELEPVVSLVDVLEEDEELENEACAVLGGSDSEKCSYSQGSVKRQALYACSTCTPEGEEPA 72

Query: 68  GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFKG 127
           G+C ACS  CH  H++ EL+TKRNFRCDCGNSKF +  CKL  +K   N+ N YN NF G
Sbjct: 73  GICLACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFG 132

Query: 128 SYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYEDFICPA 187
            YCIC RPYPDP+ E   EMIQC +CEDWFH  HLG      IP  E G+  +++ +C A
Sbjct: 133 LYCICKRPYPDPEDEIPDEMIQCVVCEDWFHGRHLG-----AIP-PESGD--FQEMVCQA 192

Query: 188 CSAICSFLIQYPSSIWAVGRHSAGKDSLL-------------------------SELPEN 247
           C   CSFL  Y + + AV + S   D L+                          ++PE 
Sbjct: 193 CMKRCSFLWAYAAQL-AVTKISTEDDGLVRNIDGIGDQEVIKPENGEHQDSTLKEDVPEQ 252

Query: 248 NSGSKESVKDNTQTDSCVLETSSS--------ASITAENK------------------PM 307
                  VK    ++ C   +S S         S+ AE+K                    
Sbjct: 253 GKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLNAESKSGCKLQELKAKQLIKKDTAT 312

Query: 308 FLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELKL 367
           +   NWR  LC C+ C+  Y   ++ FL DE D++  YE   K     +  ++    +  
Sbjct: 313 YWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGK---IAQATDRSDPLMDT 372

Query: 368 FDNLGHVEKIEILNGIADMKDEIRTFMESF-DSSKPITSADIHQVFENLAKKRRR 369
             ++  V+++E++    D+K E++ +++ F D    +   DI Q FE    K+RR
Sbjct: 373 LSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRR 415

BLAST of CmaCh15G012810 vs. ExPASy Swiss-Prot
Match: Q54DV0 (Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 GN=ubr7 PE=3 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 1.9e-36
Identity = 128/417 (30.70%), Postives = 190/417 (45.56%), Query Frame = 0

Query: 3   EVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTC--T 62
           E  +D+ E+  ++ E  KE E+++        GDE   CT+ KGY+  Q++F+C TC  +
Sbjct: 72  EALNDQLEEEKNLLEEAKEQEQED-------WGDE-SICTFDKGYI-NQSVFACKTCQLS 131

Query: 63  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCG---NSKFGDFICKLF------SNKD 122
            D   G C  CS+ CH  H++ EL+ KRNFRCDCG         F C+L        N +
Sbjct: 132 NDKLFGFCYGCSMHCHLYHDVYELFNKRNFRCDCGTKIQEPNNSFKCQLSGILKEDDNNN 191

Query: 123 VENANNS-----------------------------------------YNHNFKGSYCIC 182
           V N NNS                                         YNHNFKG YC C
Sbjct: 192 VNNINNSNNTTTTTTTTTTTTTTTNNNHLNDIDIGSYDKSQILNERNHYNHNFKGKYCYC 251

Query: 183 NRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGL-SSIDEIPRDEEGEPLYEDFICPACSAI 242
           + PY   D +E  +MIQC  CEDWFHE  L L S++ +IP   E    + D IC  C + 
Sbjct: 252 DSPY---DYKE--DMIQCIFCEDWFHENCLKLNSNVTDIPSPGE----FSDLICADCLSK 311

Query: 243 CSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETSSSASITA 302
             FL+ YP       R     D ++  + +NN+ +  S   N     C +E      IT 
Sbjct: 312 NQFLLLYPQI-----RCYIENDHII--IGDNNNNNNNS---NNSNSICKVE---GGVITP 371

Query: 303 ENK-PMFLSKNWRGALCRCEKCINNYKLKNISFLVD-EEDSIAEYEKIAKQKREEK-LQE 362
             K  +F  + W+  LC C KC   YK K + FL + +E+S+ +  K   +  + K +  
Sbjct: 372 NKKYDLFCKELWKDELCSCLKCKEIYKDKKVEFLFEKDENSLKKKNKTVDENLDNKPVNV 431

BLAST of CmaCh15G012810 vs. ExPASy Swiss-Prot
Match: Q09329 (Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mlo2 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.2e-36
Identity = 111/368 (30.16%), Postives = 175/368 (47.55%), Query Frame = 0

Query: 7   DETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDGNA- 66
           +ET   +++++Y+++  E E EA  VL       CTYS GY+K Q +++CLTC     + 
Sbjct: 2   EETAHELTVKQYVEQQRELEREAREVL-PYSFDTCTYSMGYLK-QPLYACLTCQKASGSL 61

Query: 67  -GVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNFK 126
             VC +CS+SCH  H++V+L+ KR+FRCDCG ++     C L  + D   + N YNHNF+
Sbjct: 62  NAVCYSCSISCHADHDLVDLFNKRHFRCDCGTTRTHSIPCNLRKSVDECGSENDYNHNFE 121

Query: 127 GSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYEDFICP 186
           G +CIC+  Y +P+ EE   M QC +CEDWFHE+ L  ++      D E    +E  +C 
Sbjct: 122 GRFCICDTVY-NPETEEGT-MFQCILCEDWFHEKCLQKTNKGIAIPDAE---TFEWLVCS 181

Query: 187 ACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETSSS 246
            CS                      +D LL++  E+ +G+                    
Sbjct: 182 ECS-------------------EKYRDHLLNQKHESIAGT-------------------- 241

Query: 247 ASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDE---------EDSIAEYEKI 306
                E  P+FLS+N+R  LC CE CI+   L+    + +E         ED I+E  + 
Sbjct: 242 -----ERAPLFLSENFRENLCPCESCISLRNLEMPMLVAEEPIYEPPEDSEDGISEMNED 301

Query: 307 AKQKR---EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESF-DSSKPIT 360
             +     E+ +       L++ D L  V+  E +     +K E+  F+  F   ++ +T
Sbjct: 302 PSESGEMIEQVISSTMNDVLRILDRLPRVQANESVYAYNRLKSELTDFLTPFARENRVVT 318

BLAST of CmaCh15G012810 vs. ExPASy TrEMBL
Match: A0A6J1I2R3 (putative E3 ubiquitin-protein ligase UBR7 OS=Cucurbita maxima OX=3661 GN=LOC111469061 PE=4 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 3.0e-215
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE
Sbjct: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 370

BLAST of CmaCh15G012810 vs. ExPASy TrEMBL
Match: A0A6J1EM95 (putative E3 ubiquitin-protein ligase UBR7 OS=Cucurbita moschata OX=3662 GN=LOC111435811 PE=4 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 7.4e-206
Identity = 357/370 (96.49%), Postives = 358/370 (96.76%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FIC  CSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN         NTQTDSCVLE
Sbjct: 181 FICSVCSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN---------NTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASI AENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASIIAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLF+NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFNNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 361

BLAST of CmaCh15G012810 vs. ExPASy TrEMBL
Match: A0A0A0KQR4 (UBR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G149850 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 6.5e-194
Identity = 345/414 (83.33%), Postives = 355/414 (85.75%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTY+KGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYNKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F+CKLFSNKDVEN+NNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGNFVCKLFSNKDVENSNNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRH---------------------------------- 240
           FIC ACSA+CSFL QYPSSIWAVGR                                   
Sbjct: 181 FICKACSAVCSFLSQYPSSIWAVGRQSDTSMDVSNKIDASELPASTSVKHNDDVNLHDSA 240

Query: 241 ----------SAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETSSSASITAENKPMFL 300
                     SAGKDSLLSELPEN S S E +KDN QTDSCVL TS SASI  ENKPMFL
Sbjct: 241 NADPTVDTDKSAGKDSLLSELPENPS-SSEPIKDNNQTDSCVLGTSLSASIIVENKPMFL 300

Query: 301 SKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFD 360
           SKNWRGALCRCEKCINNYKL NISFL+DEEDSIAEYEK+AKQKREEKL +QEGAE KLFD
Sbjct: 301 SKNWRGALCRCEKCINNYKLNNISFLLDEEDSIAEYEKMAKQKREEKLLQQEGAESKLFD 360

Query: 361 NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFENLAKKRRRTE 371
           NLGHVEKIEILNGIADMKDEIRTF+ESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 NLGHVEKIEILNGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 413

BLAST of CmaCh15G012810 vs. ExPASy TrEMBL
Match: A0A5A7TMU5 (Putative E3 ubiquitin-protein ligase UBR7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001830 PE=4 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 3.0e-191
Identity = 341/414 (82.37%), Postives = 353/414 (85.27%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTY+KGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYNKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFG+F+CKLFSNKDVEN++NSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGNFVCKLFSNKDVENSDNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLS IDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSFIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRH---------------------------------- 240
           FIC ACSA+CSFL  YPSSIWAVGR                                   
Sbjct: 181 FICKACSAVCSFLSLYPSSIWAVGRQSDTSLDVSNKIDASELPASTSLKHNDDVNLHDSA 240

Query: 241 ----------SAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETSSSASITAENKPMFL 300
                     SAGKDSLLSELPEN S SK  +KDN+QTDSCVL TS SASI  ENKPMFL
Sbjct: 241 NVHPTVDTDKSAGKDSLLSELPENPSSSK-PIKDNSQTDSCVLGTSLSASIIVENKPMFL 300

Query: 301 SKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFD 360
           SKNWRGALCRCEKCIN YKL NISFL+DEEDSIAEYEK+AKQKREEKL +QEGAE KLFD
Sbjct: 301 SKNWRGALCRCEKCINTYKLNNISFLLDEEDSIAEYEKMAKQKREEKLLQQEGAESKLFD 360

Query: 361 NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFENLAKKRRRTE 371
           NLGHVEKIEILNGIADMKDEIRTF+ESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 NLGHVEKIEILNGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 413

BLAST of CmaCh15G012810 vs. ExPASy TrEMBL
Match: A0A6J1CWZ9 (putative E3 ubiquitin-protein ligase UBR7 OS=Momordica charantia OX=3673 GN=LOC111015345 PE=4 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.2e-191
Identity = 341/417 (81.77%), Postives = 353/417 (84.65%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDF CKL SNK VENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFFCKLTSNKAVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCC+CEDWFHEEHLGL+SIDEIPRDEEG P YED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCLCEDWFHEEHLGLNSIDEIPRDEEGVPQYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRH---------------------------------- 240
           FIC ACSA+CSFL QYPSSIWAVGRH                                  
Sbjct: 181 FICKACSAVCSFLCQYPSSIWAVGRHPDISLDVSVKNDVPEQTLSVCTSVKHDNDGNPDD 240

Query: 241 ------------SAGKDSLLSELPENNSGSKESVKDNTQTDSCVLETS-SSASITAENKP 300
                       SAGKDS LSELPENNS S E++ +  QT SCVL TS SSAS+  ENKP
Sbjct: 241 SANADLTVAPEESAGKDSFLSELPENNSCSNETINEVDQTASCVLGTSQSSASVVVENKP 300

Query: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKREEKLQEQEGAELK 360
           MFLSKNWRGALCRCEKCINNYKLKNISFL+DEEDSIAEYEKIAKQKREEKLQ+QEGAELK
Sbjct: 301 MFLSKNWRGALCRCEKCINNYKLKNISFLLDEEDSIAEYEKIAKQKREEKLQQQEGAELK 360

Query: 361 LFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFENLAKKRRRTE 371
           +FDNLGHVEKIEIL+GIADMKDEIRTF+ESFDSSKPITSADIHQVFENLAKKRRRTE
Sbjct: 361 MFDNLGHVEKIEILSGIADMKDEIRTFLESFDSSKPITSADIHQVFENLAKKRRRTE 417

BLAST of CmaCh15G012810 vs. NCBI nr
Match: XP_022970058.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita maxima])

HSP 1 Score: 757.3 bits (1954), Expect = 6.2e-215
Identity = 370/370 (100.00%), Postives = 370/370 (100.00%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE
Sbjct: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 370

BLAST of CmaCh15G012810 vs. NCBI nr
Match: XP_022929140.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita moschata] >XP_022929141.1 putative E3 ubiquitin-protein ligase UBR7 [Cucurbita moschata])

HSP 1 Score: 726.1 bits (1873), Expect = 1.5e-205
Identity = 357/370 (96.49%), Postives = 358/370 (96.76%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FIC  CSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN         NTQTDSCVLE
Sbjct: 181 FICSVCSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN---------NTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASI AENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASIIAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLF+NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFNNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 361

BLAST of CmaCh15G012810 vs. NCBI nr
Match: XP_023550054.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 723.4 bits (1866), Expect = 9.9e-205
Identity = 355/370 (95.95%), Postives = 358/370 (96.76%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVE+QELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEDQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FICPACS ICSFLIQYPSSIWAVGRHSAGK+SLLSELPENN         NTQTDSCVLE
Sbjct: 181 FICPACSEICSFLIQYPSSIWAVGRHSAGKNSLLSELPENN---------NTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASI AENKPMFLSKNWR ALCRCEKCI+NYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASIIAENKPMFLSKNWRSALCRCEKCIHNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 361

BLAST of CmaCh15G012810 vs. NCBI nr
Match: KAG6579557.1 (putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 723.4 bits (1866), Expect = 9.9e-205
Identity = 356/370 (96.22%), Postives = 358/370 (96.76%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120
           PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN
Sbjct: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYN 120

Query: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180
           HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED
Sbjct: 121 HNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRDEEGEPLYED 180

Query: 181 FICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDNTQTDSCVLE 240
           FIC ACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN         NTQTDSCVLE
Sbjct: 181 FICSACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN---------NTQTDSCVLE 240

Query: 241 TSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAEYEKIAKQKR 300
           TSSSASI AENKPMFLSKNWR ALCRCEKCI+NYKLKNISFLVDEEDSIAEYEKIAKQKR
Sbjct: 241 TSSSASIIAENKPMFLSKNWRSALCRCEKCIHNYKLKNISFLVDEEDSIAEYEKIAKQKR 300

Query: 301 EEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360
           EEKLQEQEGAELKLF+NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE
Sbjct: 301 EEKLQEQEGAELKLFNNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPITSADIHQVFE 360

Query: 361 NLAKKRRRTE 371
           NLAKKRRRTE
Sbjct: 361 NLAKKRRRTE 361

BLAST of CmaCh15G012810 vs. NCBI nr
Match: KAG7017017.1 (putative E3 ubiquitin-protein ligase UBR7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 693.3 bits (1788), Expect = 1.1e-195
Identity = 347/379 (91.56%), Postives = 350/379 (92.35%), Query Frame = 0

Query: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60
           MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT
Sbjct: 1   MAEVFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCT 60

Query: 61  PDGNAGVCTACSLSCHDGHEIVELWTKRNFR---------CDCGNSKFGDFICKLFSNKD 120
           PDGNAGVCTACSLSCHDGHE+        FR         CDCGNSKFGDFICKLFSNKD
Sbjct: 61  PDGNAGVCTACSLSCHDGHEVYMFLEGTLFRHAGVYVFFLCDCGNSKFGDFICKLFSNKD 120

Query: 121 VENANNSYNHNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRD 180
           VENANNSYNHNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRD
Sbjct: 121 VENANNSYNHNFKGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSIDEIPRD 180

Query: 181 EEGEPLYEDFICPACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENNSGSKESVKDN 240
           EEGEPLYEDFIC ACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN         N
Sbjct: 181 EEGEPLYEDFICSACSAICSFLIQYPSSIWAVGRHSAGKDSLLSELPENN---------N 240

Query: 241 TQTDSCVLETSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFLVDEEDSIAE 300
           TQTDSCVLETSSSASI AENKPMFLSKNWR ALCRCEKCI+NYKLKNISFLVDEEDSIAE
Sbjct: 241 TQTDSCVLETSSSASIIAENKPMFLSKNWRSALCRCEKCIHNYKLKNISFLVDEEDSIAE 300

Query: 301 YEKIAKQKREEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPIT 360
           YEKIAKQKREEKLQEQEGAELKLF+NLGHVEKIEILNGIADMKDEIRTFMESFDSSKPIT
Sbjct: 301 YEKIAKQKREEKLQEQEGAELKLFNNLGHVEKIEILNGIADMKDEIRTFMESFDSSKPIT 360

Query: 361 SADIHQVFENLAKKRRRTE 371
           SADIHQVFENLAKKRRRTE
Sbjct: 361 SADIHQVFENLAKKRRRTE 370

BLAST of CmaCh15G012810 vs. TAIR 10
Match: AT4G23860.1 (PHD finger protein-related )

HSP 1 Score: 454.5 bits (1168), Expect = 8.0e-128
Identity = 230/448 (51.34%), Postives = 284/448 (63.39%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +KD+EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSID----EIPRDEEGEPLYE 183
           KG YC C+RPYPDP+VEEQVEMIQCC+CEDWFHEEHLGL+  D    +IPRDEE EP+YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICPACSAICSFLIQYPSSIWAVGR-HSAGKDSLLSEL--------------PENNSGS 243
           DFIC  CS  CSFL  YP ++W V +  S G  +  SE               PEN + +
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 KESV-------------------------------------------------------- 303
           ++SV                                                        
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 ------KDNTQTDSCVLETSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFL 363
                  +N     CV+ T  ++    E KP+FL+KNWR  LCRCEKC+  YK + +S+L
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCPEFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSYL 364

Query: 364 VDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFME 371
           +D ED+I EYEK AK+KR EKL++QEG  L L +NL HV K+E+L+GI D +D ++  ME
Sbjct: 365 LDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLME 424

BLAST of CmaCh15G012810 vs. TAIR 10
Match: AT4G23860.2 (PHD finger protein-related )

HSP 1 Score: 454.5 bits (1168), Expect = 8.0e-128
Identity = 230/448 (51.34%), Postives = 284/448 (63.39%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +KD+EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSID----EIPRDEEGEPLYE 183
           KG YC C+RPYPDP+VEEQVEMIQCC+CEDWFHEEHLGL+  D    +IPRDEE EP+YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICPACSAICSFLIQYPSSIWAVGR-HSAGKDSLLSEL--------------PENNSGS 243
           DFIC  CS  CSFL  YP ++W V +  S G  +  SE               PEN + +
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 KESV-------------------------------------------------------- 303
           ++SV                                                        
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 ------KDNTQTDSCVLETSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFL 363
                  +N     CV+ T  ++    E KP+FL+KNWR  LCRCEKC+  YK + +S+L
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCPEFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSYL 364

Query: 364 VDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFME 371
           +D ED+I EYEK AK+KR EKL++QEG  L L +NL HV K+E+L+GI D +D ++  ME
Sbjct: 365 LDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLME 424

BLAST of CmaCh15G012810 vs. TAIR 10
Match: AT4G23860.3 (PHD finger protein-related )

HSP 1 Score: 454.5 bits (1168), Expect = 8.0e-128
Identity = 230/448 (51.34%), Postives = 284/448 (63.39%), Query Frame = 0

Query: 4   VFDDETEQTISIQEYLKEVEEQELEADLVLGGDEGKECTYSKGYMKRQAIFSCLTCTPDG 63
           VF+DE E TI+I EY++ ++ +EL ADLVLGGDEG ECT+ KGYMKRQAIFSC+TCTP+G
Sbjct: 5   VFEDEAEGTITINEYIERLDAEELAADLVLGGDEGDECTFPKGYMKRQAIFSCITCTPEG 64

Query: 64  NAGVCTACSLSCHDGHEIVELWTKRNFRCDCGNSKFGDFICKLFSNKDVENANNSYNHNF 123
           NAG+CTAC LSCHDGHE++ELWTKRNFRCDCGNSKFG   CKL  +KD+EN+ NSYNHNF
Sbjct: 65  NAGICTACCLSCHDGHELLELWTKRNFRCDCGNSKFGTLACKLLPSKDIENSENSYNHNF 124

Query: 124 KGSYCICNRPYPDPDVEEQVEMIQCCMCEDWFHEEHLGLSSID----EIPRDEEGEPLYE 183
           KG YC C+RPYPDP+VEEQVEMIQCC+CEDWFHEEHLGL+  D    +IPRDEE EP+YE
Sbjct: 125 KGLYCTCDRPYPDPNVEEQVEMIQCCLCEDWFHEEHLGLTPSDSVGSQIPRDEESEPIYE 184

Query: 184 DFICPACSAICSFLIQYPSSIWAVGR-HSAGKDSLLSEL--------------PENNSGS 243
           DFIC  CS  CSFL  YP ++W V +  S G  +  SE               PEN + +
Sbjct: 185 DFICQNCSPACSFLTLYPENLWVVAKVDSTGSANACSETIELDKNHMDSEPGQPENGTDA 244

Query: 244 KESV-------------------------------------------------------- 303
           ++SV                                                        
Sbjct: 245 EKSVVGKCSETISDSEPGQPENGTEAEKSVVQKCSEKIDESEAGQPENSTEAEKFVVRKC 304

Query: 304 ------KDNTQTDSCVLETSSSASITAENKPMFLSKNWRGALCRCEKCINNYKLKNISFL 363
                  +N     CV+ T  ++    E KP+FL+KNWR  LCRCEKC+  YK + +S+L
Sbjct: 305 SEKIDGSENVPAAGCVIRTDLNSCPEFEKKPLFLTKNWRNILCRCEKCLEMYKQRKVSYL 364

Query: 364 VDEEDSIAEYEKIAKQKREEKLQEQEGAELKLFDNLGHVEKIEILNGIADMKDEIRTFME 371
           +D ED+I EYEK AK+KR EKL++QEG  L L +NL HV K+E+L+GI D +D ++  ME
Sbjct: 365 LDAEDTIVEYEKKAKEKRTEKLEKQEGEALDLLNNLDHVSKVELLHGIKDFQDGLQGLME 424

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8BU043.2e-6536.63Putative E3 ubiquitin-protein ligase UBR7 OS=Mus musculus OX=10090 GN=Ubr7 PE=1 ... [more]
Q8N8061.4e-6335.66Putative E3 ubiquitin-protein ligase UBR7 OS=Homo sapiens OX=9606 GN=UBR7 PE=1 S... [more]
Q54DV01.9e-3630.70Putative E3 ubiquitin-protein ligase ubr7 OS=Dictyostelium discoideum OX=44689 G... [more]
Q093293.2e-3630.16Protein mlo2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1I2R33.0e-215100.00putative E3 ubiquitin-protein ligase UBR7 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1EM957.4e-20696.49putative E3 ubiquitin-protein ligase UBR7 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0KQR46.5e-19483.33UBR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G149850 PE... [more]
A0A5A7TMU53.0e-19182.37Putative E3 ubiquitin-protein ligase UBR7 OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1CWZ95.2e-19181.77putative E3 ubiquitin-protein ligase UBR7 OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
XP_022970058.16.2e-215100.00putative E3 ubiquitin-protein ligase UBR7 [Cucurbita maxima][more]
XP_022929140.11.5e-20596.49putative E3 ubiquitin-protein ligase UBR7 [Cucurbita moschata] >XP_022929141.1 p... [more]
XP_023550054.19.9e-20595.95putative E3 ubiquitin-protein ligase UBR7 [Cucurbita pepo subsp. pepo][more]
KAG6579557.19.9e-20596.22putative E3 ubiquitin-protein ligase UBR7, partial [Cucurbita argyrosperma subsp... [more]
KAG7017017.11.1e-19591.56putative E3 ubiquitin-protein ligase UBR7 [Cucurbita argyrosperma subsp. argyros... [more]
Match NameE-valueIdentityDescription
AT4G23860.18.0e-12851.34PHD finger protein-related [more]
AT4G23860.28.0e-12851.34PHD finger protein-related [more]
AT4G23860.38.0e-12851.34PHD finger protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 282..302
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 214..236
NoneNo IPR availablePANTHERPTHR13513:SF9E3 UBIQUITIN-PROTEIN LIGASE UBR7-RELATEDcoord: 5..369
NoneNo IPR availableCDDcd15542PHD_UBR7coord: 127..186
e-value: 1.09512E-22
score: 87.8082
IPR003126Zinc finger, UBR-typeSMARTSM00396push_1coord: 39..108
e-value: 2.4E-20
score: 83.6
IPR003126Zinc finger, UBR-typePFAMPF02207zf-UBRcoord: 41..105
e-value: 1.7E-14
score: 53.7
IPR003126Zinc finger, UBR-typePROSITEPS51157ZF_UBRcoord: 39..109
score: 9.127059
IPR001965Zinc finger, PHD-typeSMARTSM00249PHD_3coord: 127..187
e-value: 5.2E-6
score: 36.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 123..189
e-value: 1.7E-12
score: 48.7
IPR040204E3 ubiquitin-protein ligase UBR7PANTHERPTHR13513E3 UBIQUITIN-PROTEIN LIGASE UBR7coord: 5..369
IPR011011Zinc finger, FYVE/PHD-typeSUPERFAMILY57903FYVE/PHD zinc fingercoord: 115..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh15G012810.1CmaCh15G012810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding