CmaCh15G012300 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh15G012300
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Tyrosine--tRNA ligase
Location	Cma_Chr15: 7802621 .. 7805264 (+)
RNA-Seq Expression	CmaCh15G012300
Synteny	CmaCh15G012300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGACGGGAGAACCCTCACAAGCTCCATCCCAAGATTTACAGTCCCTCTCAATCGCTGACGCTAAGGTGCAGGAGCCGGGTTCTTCATCAAACTCTTCAAATCAGTAAGAAGCTTTTGGTTTGAGATACTTGTTGTTTTCGTCTCAATTTCGTCGTCGTTTTGTATCTGAATCGGACATTACTTGGTTTGTTATGTTTTTTTTTTTTTTTCTTTCTGCAGGTTGAGTTTGGAGGAGAAGTTTAAAATTGTTAGAAGTATTGGGGAAGAATGTATCCAAGAGGATGAGCTGCGGAATTTGCTCGAGAAGAAGCCGGAACCTGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGTAAAGATTACATGATTCTTTGAGTTTGGCCTTCAAATTGTTATTTGCCTTCCTCAATGAATTGTGTTGCATTTCGATGCATAATGGAAGCCCTAAACTAAGTGAATTGGGTTTAGTTTTCCGTGTGAATCTATCGGAAAAAATGGGACACCAACTAATTGTTAGATTAATGTAAAATGAACAATTCCTATGTAAGTTTCTTCCACTTAAGGGTTTTTGAACATGAGGAGATGATTTCTATGCCCTTGTTCGCTTCTACAGGGAGTGATGAAGACAATAAATGTCAACAAGCTTACTGCTTCTGGGTGCCGAGTGAAAATATGGATTGCAGACTGGTTTGCTCAGCTTAACAACAAAATGGGGGGTGATCTGAAAAAAATTGAGACTGTTGGCCGCTACCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCGTCGAAGGAGATTAACTCAAGGGCGTCGGAATACTGGCCCCTTGTAATGGATATCGCTCGGAAAAACAAACTTCCAAGGATAATTAGGTATATATTTGAGACGGGTGCTCGTTGCGTACTAATGTTTAATCTGCAACGGTGTCAACTCATGGTTTGCCTCCCCCCTTGATCCCTCTCCTGCAGGTGTAGCCAAATTATGGGAAGAACTGAACAAGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTGCGGACATTTTTTTCCTTAAGGTTTTTTCTCTTTCGACTTTCTGTAGTATGTTATGCATTTTTCTCCTCATATCATAAGGAGGCCAAAATGTAATTTGGTACAAATGCTGCAAAATATATTTGTCTGGCAATGAAATTTTCATGGTGATATAGGTTGTCCATTTTTTTACTCTCCCCTACTATGTTGGAATGTATGGAGAGATTATCATGATTTTTTGTTAATTAGTTGTTCCCTTCCATTGCCACAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAGAACAAGCCTATCATCCTATCGCACCGTATGCTCTAAAATGCGTTGACATTAGTTCTTATTAATTTAGCCAACACTTACGATATAGGCAAAGTAATATGGTGTCAATGGTGGTTGCAGATATGTTACCTGGCTTACAGCAGGGGCAGGAAAAGATGTCAAAAAGTGATCCATCGTCCTCTATATTTATGGAAGATGATGAGGTAAGGCATTATATAGGGGAAATTAAGAATCCTATGTGAGTAGGAAATTGGGGTGGTGGTGCAGTTTGGCTAGCGTTTAGGTCTCATAGCTATAATTAAGAATCCCGTTTGAGGGGAATCAAATTGCATCTACTATTTTAATGTTTGTGCTTAGTTTTAGGTTGACTTGACTGACGTGTCCGACTCTGATGCCAGCTTGTAATATTCTGGATTTACTGTCTAACTTGTTCATCTGGCTATAGGCAGAAGTGAACTTGAAGATAAAGAAGGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAGTACATAAAGTATCTCATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGTAAGTTTTTGAGGAGATTTAGTTGCTCATTTTGAAGTTCTAAACTTTTTTATTGTTTTTTCACCTATATCATGATCCTAGATGCTAGTTATAATAATTTATCGTTGCTCTGCATTCATTTGTTGTTTTGAAATATTGCAGGACCTTCACAACCTTCGAAGAATTAGTTGCTGACTACGCATCAGGAGTACTGCATCCAGGTGATCTTAAACCAGCTTTGTCCAAGGCTTTGAATAAGATATTGGAGGTATGTTTTCATTTCTACTCAATATCTTCTCATACTCCGTTATGCTTCCTCGATCGAGGGAGTTAATCCCATGTGCTTCTTATTGTGATAGGAATCATTGTAGTTATCTCTTTTCCTCGTCTGTCTTTGGCATTTACCCATGTTCTTGCAATTTTATCTGAAAACTGCATCTCAGCATAACTTAAGAAGCTTCGTTTTTTTCACCATGAAAATATAAACAGCTTTTTAGGGAAAGGGTAGTGATGCAGCTAGAATATTTACTCAATTCTGTCTACTCATATTATAAAGCTAAGATCTGTATCATCCATCTGCAGCCTGTACGAGATCACTTCAAAAATGATAGCAATGCCAAAGATTTATTGAAGAGGGTCAAGGTATGTGCTTAA

mRNA sequence

ATGGCGACGGGAGAACCCTCACAAGCTCCATCCCAAGATTTACAGTCCCTCTCAATCGCTGACGCTAAGGTGCAGGAGCCGGGTTCTTCATCAAACTCTTCAAATCAGTTGAGTTTGGAGGAGAAGTTTAAAATTGTTAGAAGTATTGGGGAAGAATGTATCCAAGAGGATGAGCTGCGGAATTTGCTCGAGAAGAAGCCGGAACCTGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGACAATAAATGTCAACAAGCTTACTGCTTCTGGGTGCCGAGTGAAAATATGGATTGCAGACTGGTTTGCTCAGCTTAACAACAAAATGGGGGGTGATCTGAAAAAAATTGAGACTGTTGGCCGCTACCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCGTCGAAGGAGATTAACTCAAGGGCGTCGGAATACTGGCCCCTTGTAATGGATATCGCTCGGAAAAACAAACTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAACTGAACAAGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTGCGGACATTTTTTTCCTTAAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAGAACAAGCCTATCATCCTATCGCACCATATGTTACCTGGCTTACAGCAGGGGCAGGAAAAGATGTCAAAAAGTGATCCATCGTCCTCTATATTTATGGAAGATGATGAGGCAGAAGTGAACTTGAAGATAAAGAAGGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAGTACATAAAGTATCTCATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCACAACCTTCGAAGAATTAGTTGCTGACTACGCATCAGGAGTACTGCATCCAGGTGATCTTAAACCAGCTTTGTCCAAGGCTTTGAATAAGATATTGGAGCCTGTACGAGATCACTTCAAAAATGATAGCAATGCCAAAGATTTATTGAAGAGGGTCAAGGTATGTGCTTAA

Coding sequence (CDS)

ATGGCGACGGGAGAACCCTCACAAGCTCCATCCCAAGATTTACAGTCCCTCTCAATCGCTGACGCTAAGGTGCAGGAGCCGGGTTCTTCATCAAACTCTTCAAATCAGTTGAGTTTGGAGGAGAAGTTTAAAATTGTTAGAAGTATTGGGGAAGAATGTATCCAAGAGGATGAGCTGCGGAATTTGCTCGAGAAGAAGCCGGAACCTGTGTGCTATGATGGGTTCGAACCATCTGGGAGAATGCATATTGCTCAGGGAGTGATGAAGACAATAAATGTCAACAAGCTTACTGCTTCTGGGTGCCGAGTGAAAATATGGATTGCAGACTGGTTTGCTCAGCTTAACAACAAAATGGGGGGTGATCTGAAAAAAATTGAGACTGTTGGCCGCTACCTAATTGAGATATGGAAAGCAGTTGGGATGGATCTGGAAGGAGGTAAGGTTGAATTTTTGTGGTCGTCGAAGGAGATTAACTCAAGGGCGTCGGAATACTGGCCCCTTGTAATGGATATCGCTCGGAAAAACAAACTTCCAAGGATAATTAGGTGTAGCCAAATTATGGGAAGAACTGAACAAGATGAGTTGACTGCTGCCCAAATTCTCTACCCATGCATGCAATGTGCGGACATTTTTTTCCTTAAGGCTGATATCTGTCAACTGGGAATGGATCAACGGAAAGTAAATGTACTTGCAAGAGAGTATTGTGATGACATCAAGAGGAAGAACAAGCCTATCATCCTATCGCACCATATGTTACCTGGCTTACAGCAGGGGCAGGAAAAGATGTCAAAAAGTGATCCATCGTCCTCTATATTTATGGAAGATGATGAGGCAGAAGTGAACTTGAAGATAAAGAAGGCATATTGCCCTCCATTGATCGTGGAAGGGAATCCATGCTTAGAGTACATAAAGTATCTCATTTTCCCTTGGTTCAAGGAGTTCACTGTGGAACGAAATGCAGATCATGGTGGAAATAAGACCTTCACAACCTTCGAAGAATTAGTTGCTGACTACGCATCAGGAGTACTGCATCCAGGTGATCTTAAACCAGCTTTGTCCAAGGCTTTGAATAAGATATTGGAGCCTGTACGAGATCACTTCAAAAATGATAGCAATGCCAAAGATTTATTGAAGAGGGTCAAGGTATGTGCTTAA

Protein sequence

MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELRNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVKVCA

Homology

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BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot
Match: Q8S9J2 (Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g33840 PE=2 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 1.1e-169
Identity = 286/381 (75.07%), Postives = 331/381 (86.88%), Query Frame = 0


Query: 3   TGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLS--LEEKFKIVRSIGEECIQEDELR 62
           + E   A S +++++S++  +     SSS+   Q+S  +E+++KIVRSIGEECIQE+EL+
Sbjct: 5   SSEIVNALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELK 64

Query: 63  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 122
           NLL KK  P+CYDGFEPSGRMHIAQGVMK INVNK+T++GCRVKIWIADWFAQLNNKMGG
Sbjct: 65  NLLAKKAAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGG 124

Query: 123 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 182
           DLKKI  VG Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIARKNKLPRI
Sbjct: 125 DLKKIRVVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRI 184

Query: 183 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 242
           +RC QIMGR+E DEL+AAQILYPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 185 LRCVQIMGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKR 244

Query: 243 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 302
           KNKPIILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAYCPP +V+GNPCL
Sbjct: 245 KNKPIILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCL 304

Query: 303 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 362
           EYIKY+I PWF EFTVERN ++GGNKT+ +FE++ ADY SG LHPGDLK  L  ALNKIL
Sbjct: 305 EYIKYIILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKIL 364

Query: 363 EPVRDHFKNDSNAKDLLKRVK 382
           +PVRDHFK D+ AK+LLK++K
Sbjct: 365 QPVRDHFKTDARAKNLLKQIK 379

[Close]

BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot
Match: F4HWL4 (Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g28350 PE=1 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 1.9e-153
Identity = 263/363 (72.45%), Postives = 304/363 (83.75%), Query Frame = 0


Query: 21  DAKVQEPGSSSNSSNQLS--LEEKFKIVRSIGEECIQEDELRNLLEKKPEPVCYDGFEPS 80
           D  +    SSS +  Q+S   E K+KIVRSIGEECIQEDEL+NLL KKP P+CYDGFEPS
Sbjct: 383 DLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPS 442

Query: 81  GRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKA 140
           GRMHIAQGVMK  NVNKLT++GC+VKIWIADWFAQLNNK+GGDL++I  VG Y  EIW+A
Sbjct: 443 GRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQA 502

Query: 141 VGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAA 200
            GM+    KVEFLW+S EIN + S+YWPLVMDIAR+N L RI+RC QIMGR+E + L+AA
Sbjct: 503 GGMN--NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAA 562

Query: 201 QILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQG 260
           QILYPCMQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLPGLQQG
Sbjct: 563 QILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLPGLQQG 622

Query: 261 QEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVER 320
           QEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F EF VE 
Sbjct: 623 QEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNEFKVE- 682

Query: 321 NADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLK 380
           +  +GGNKTF +FE++VADY +G LHP DLK AL KALN  L+PVRDHFK +  AK+LL+
Sbjct: 683 SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQPVRDHFKTNERAKNLLE 742

Query: 381 RVK 382
           +VK
Sbjct: 743 QVK 742

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BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot
Match: Q5UPJ7 (Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.7e-72
Identity = 154/345 (44.64%), Postives = 214/345 (62.03%), Query Frame = 0


Query: 40  EEKFKIVRSIGEECIQEDELRNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTAS 99
           E +   + SI EEC   D L+ L++       Y+GFEPSGR+HIAQ ++  +N N +   
Sbjct: 10  EHRLTQLLSIAEECETLDRLKQLVDSGRIFTAYNGFEPSGRIHIAQALITVMNTNNIIEC 69

Query: 100 GCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINS 159
           G ++ I+IADWFA++N KM GD+ KI  +GRY IE++KA G++L+G    F+W+S+ I S
Sbjct: 70  GGQMIIYIADWFAKMNLKMNGDINKIRELGRYFIEVFKACGINLDG--TRFIWASEFIAS 129

Query: 160 RASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAAQILYPCMQCADIFFL---KAD 219
             S Y   ++DIA  + + R+ RC QIMGR E D L A+QI YPCMQ AD+F L     D
Sbjct: 130 NPS-YIERMLDIAEFSTISRVKRCCQIMGRNESDCLKASQIFYPCMQAADVFELVPEGID 189

Query: 220 ICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDD 279
           ICQLG+DQRKVN+LA EY +D +    PI LSHHML  L   ++KMSKSDP  +IFM+D 
Sbjct: 190 ICQLGIDQRKVNMLAIEYAND-RGLKIPISLSHHMLMSLSGPKKKMSKSDPQGAIFMDDT 249

Query: 280 EAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVA 339
           E EV+ KI +AYC     + NP  EYIKYL+  WF    +         K +T  E +  
Sbjct: 250 EQEVSEKISRAYCTDETFD-NPIFEYIKYLLLRWFGTLNL-------CGKIYTDIESIQE 309

Query: 340 DYASGVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLKRVK 382
           D++S  ++  +LK  ++  +N I++ VR+HFK      +LL  VK
Sbjct: 310 DFSS--MNKRELKTDVANYINTIIDLVREHFKK-PELSELLSNVK 339

[Close]

BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot
Match: Q5JF63 (Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) OX=69014 GN=tyrS PE=3 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 4.1e-63
Identity = 137/375 (36.53%), Postives = 217/375 (57.87%), Query Frame = 0


Query: 37  LSLEEKFKIV-RSIGEECIQEDELRNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNK 96
           + +E K +++ +   EE + E+ LR+LLE       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIERKIELIKKKPTEELLTEENLRHLLEVGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTASGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGG---KVEFLW 156
           L  +G + +I++ADW + +N+K+GGDL+ I+ V     +      + + GG   KVEF+ 
Sbjct: 61  LQKAGVKTRIFLADWHSWINDKLGGDLEVIQKVALTYFKEGMKQSIKVMGGDPDKVEFVL 120

Query: 157 SSKEINSRASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAAQILYPCMQCADIFF 216
           +S+ ++    +YW  V+DI++   L R++R   IMGR   + +  A+++YP MQ ADIF+
Sbjct: 121 ASEILDK--GDYWQTVIDISKNVTLARMLRSITIMGRQMGEAIDFAKLIYPAMQVADIFY 180

Query: 217 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 276
               I   GMDQRK +V+A E    +K         K KP+ L HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAQKLKYHPLEWKGEKLKPVALHHHLLLGLQEPPVWPIE 240

Query: 277 ---------GQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIF 336
                     Q KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++ +Y+IF
Sbjct: 241 SEEQFKELKTQMKMSKSKPYSAVFIHDSPEEIKQKLRKAFCPAREVKYNPVLDWAEYIIF 300

Query: 337 -PWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKILEPVRDHF 383
                EFT+ R A  GG+ T+TTFEEL  D+A G LHP DLK A+++ L ++L+PVR++F
Sbjct: 301 REEPTEFTIHRPAKFGGDVTYTTFEELKKDFAEGKLHPLDLKNAVAEYLIELLKPVREYF 360

[Close]

BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot
Match: Q8U2H3 (Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=tyrS PE=3 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.4e-63
Identity = 133/375 (35.47%), Postives = 217/375 (57.87%), Query Frame = 0


Query: 37  LSLEEKFKIV-RSIGEECIQEDELRNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNK 96
           + +EE+ K+V +   EE +  + LR+L E       Y GFE SG +H+  G+M    +  
Sbjct: 1   MDIEERIKLVLKKPTEEVLTVENLRHLFEIGAPLQHYIGFEISGYIHLGTGLMAGAKIAD 60

Query: 97  LTASGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKAVGMDLEGG---KVEFLW 156
              +G + ++++ADW + +N+K+GGDL+ I+ V     ++     +++ GG   KVEF+ 
Sbjct: 61  FQKAGIKTRVFLADWHSWINDKLGGDLETIQEVALKYFKVGMEKSIEVMGGKPEKVEFVL 120

Query: 157 SSKEINSRASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAAQILYPCMQCADIFF 216
           +S+ +     +YW  V+DI++   L R++R   IMGR   + +  A+++YP MQ ADIF+
Sbjct: 121 ASEILEK--GDYWQTVIDISKNVTLSRVLRSITIMGRKMGEAIDFAKLIYPMMQVADIFY 180

Query: 217 LKADICQLGMDQRKVNVLAREYCDDIK--------RKNKPIILSHHMLPGLQQ------- 276
               I   GMDQRK +V+A E  + ++         K KP+ + HH+L GLQ+       
Sbjct: 181 QGVTIAHAGMDQRKAHVIAIEVAEKLRYHPIIHNGEKLKPVAVHHHLLLGLQEPPKWPIE 240

Query: 277 ---------GQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIF 336
                     + KMSKS P S++F+ D   E+  K++KA+CP   V+ NP L++++YL+F
Sbjct: 241 SEEEFKEIKAEMKMSKSKPYSAVFIHDSPEEIREKLRKAFCPAREVKYNPVLDWVEYLVF 300

Query: 337 -PWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKILEPVRDHF 383
                EFT+ R A +GG+ T+TTFEEL  D+A G LHP DLK A+++ L  +LEP+R +F
Sbjct: 301 REEPTEFTIHRPAKYGGDVTYTTFEELKRDFAEGKLHPLDLKNAVAEYLIDLLEPIRKYF 360

[Close]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL
Match: A0A6J1HXR2 (Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 5.0e-221
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR
Sbjct: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL
Match: A0A6J1ETE5 (Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.3e-218
Identity = 377/381 (98.95%), Postives = 379/381 (99.48%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPSQDLQSLSIA+AKVQEPGSSS+SSNQLSLEEKFKIVRSIGEECIQEDELR
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL
Match: A0A5A7THE1 (Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002540 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 6.5e-213
Identity = 363/383 (94.78%), Postives = 374/383 (97.65%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPS+DLQSLSI+DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 282

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 283 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 342

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 343 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 402

Query: 361 EPVRDHFKNDSNAKDLLKRVKVC 384
           EPVRDHFKNDSNAKDLLKRVKVC
Sbjct: 403 EPVRDHFKNDSNAKDLLKRVKVC 425

[Close]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL
Match: A0A1S3ATS8 (Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.1e-211
Identity = 361/381 (94.75%), Postives = 372/381 (97.64%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPS+DLQSLSI+DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL
Match: A0A0A0KMK0 (Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 3.6e-211
Identity = 359/381 (94.23%), Postives = 373/381 (97.90%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  P+QAPS+DLQSLS++DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPTQAPSEDLQSLSVSDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTF+TFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFSTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. NCBI nr
Match: XP_022969947.1 (tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita maxima])

HSP 1 Score: 776.5 bits (2004), Expect = 1.0e-220
Identity = 381/381 (100.00%), Postives = 381/381 (100.00%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR
Sbjct: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. NCBI nr
Match: XP_022929135.1 (tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita moschata] >KAG6579509.1 Tyrosine--tRNA ligase 1, cytoplasmic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016970.1 Tyrosine--tRNA ligase 1, cytoplasmic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767.7 bits (1981), Expect = 4.8e-218
Identity = 377/381 (98.95%), Postives = 379/381 (99.48%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPSQDLQSLSIA+AKVQEPGSSS+SSNQLSLEEKFKIVRSIGEECIQEDELR
Sbjct: 1   MATEAPSQAPSQDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. NCBI nr
Match: XP_023551497.1 (tyrosine--tRNA ligase 1, cytoplasmic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.1 bits (1969), Expect = 1.2e-216
Identity = 377/382 (98.69%), Postives = 379/382 (99.21%), Query Frame = 0


Query: 1   MATGEPSQAPSQ-DLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDEL 60
           MAT  PSQAPSQ DLQSLSIA+AKVQEPGSSS+SSNQLSLEEKFKIVRSIGEECIQEDEL
Sbjct: 1   MATEAPSQAPSQDDLQSLSIAEAKVQEPGSSSSSSNQLSLEEKFKIVRSIGEECIQEDEL 60

Query: 61  RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMG 120
           RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMG
Sbjct: 61  RNLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMG 120

Query: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPR 180
           GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPR
Sbjct: 121 GDLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPR 180

Query: 181 IIRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240
           IIRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK
Sbjct: 181 IIRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIK 240

Query: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300
           RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC
Sbjct: 241 RKNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPC 300

Query: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKI 360
           LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKI
Sbjct: 301 LEYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKI 360

Query: 361 LEPVRDHFKNDSNAKDLLKRVK 382
           LEPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 LEPVRDHFKNDSNAKDLLKRVK 382

[Close]

BLAST of CmaCh15G012300 vs. NCBI nr
Match: KAA0042754.1 (tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 749.6 bits (1934), Expect = 1.3e-212
Identity = 363/383 (94.78%), Postives = 374/383 (97.65%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPS+DLQSLSI+DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 43  MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 102

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 103 NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 162

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 163 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 222

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 223 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 282

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 283 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 342

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 343 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 402

Query: 361 EPVRDHFKNDSNAKDLLKRVKVC 384
           EPVRDHFKNDSNAKDLLKRVKVC
Sbjct: 403 EPVRDHFKNDSNAKDLLKRVKVC 425

[Close]

BLAST of CmaCh15G012300 vs. NCBI nr
Match: XP_008437283.1 (PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo])

HSP 1 Score: 744.6 bits (1921), Expect = 4.3e-211
Identity = 361/381 (94.75%), Postives = 372/381 (97.64%), Query Frame = 0


Query: 1   MATGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLSLEEKFKIVRSIGEECIQEDELR 60
           MAT  PSQAPS+DLQSLSI+DAKVQEP  SSNSSNQLSLEEK++IVRS+GEECIQEDELR
Sbjct: 1   MATEAPSQAPSEDLQSLSISDAKVQEPACSSNSSNQLSLEEKYQIVRSVGEECIQEDELR 60

Query: 61  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 120
           NLL+KKPEPVCYDGFEPSGRMHIAQGVMK INVNKLT++GCRVKIWIADWFAQLNNKMGG
Sbjct: 61  NLLDKKPEPVCYDGFEPSGRMHIAQGVMKAINVNKLTSAGCRVKIWIADWFAQLNNKMGG 120

Query: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 180
           DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRA EYWPLVMDIAR+NKLPRI
Sbjct: 121 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRAGEYWPLVMDIARRNKLPRI 180

Query: 181 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240
           IRCSQIMGR EQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 181 IRCSQIMGRNEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 240

Query: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 300
           KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVE NPCL
Sbjct: 241 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEANPCL 300

Query: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 360
           EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELV+DYA+G LHPGDLKPALSKALNKIL
Sbjct: 301 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVSDYAAGELHPGDLKPALSKALNKIL 360

Query: 361 EPVRDHFKNDSNAKDLLKRVK 382
           EPVRDHFKNDSNAKDLLKRVK
Sbjct: 361 EPVRDHFKNDSNAKDLLKRVK 381

[Close]

BLAST of CmaCh15G012300 vs. TAIR 10
Match: AT2G33840.1 (Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial )

HSP 1 Score: 597.4 bits (1539), Expect = 8.0e-171
Identity = 286/381 (75.07%), Postives = 331/381 (86.88%), Query Frame = 0


Query: 3   TGEPSQAPSQDLQSLSIADAKVQEPGSSSNSSNQLS--LEEKFKIVRSIGEECIQEDELR 62
           + E   A S +++++S++  +     SSS+   Q+S  +E+++KIVRSIGEECIQE+EL+
Sbjct: 5   SSEIVNALSSEMEAVSVSSTQ----ASSSSDGFQMSEEVEKRYKIVRSIGEECIQEEELK 64

Query: 63  NLLEKKPEPVCYDGFEPSGRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGG 122
           NLL KK  P+CYDGFEPSGRMHIAQGVMK INVNK+T++GCRVKIWIADWFAQLNNKMGG
Sbjct: 65  NLLAKKAAPICYDGFEPSGRMHIAQGVMKVINVNKMTSAGCRVKIWIADWFAQLNNKMGG 124

Query: 123 DLKKIETVGRYLIEIWKAVGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRI 182
           DLKKI  VG Y  EIWKA GMD    KVEFLWSS+EINS+A +YWPLVMDIARKNKLPRI
Sbjct: 125 DLKKIRVVGEYFQEIWKAAGMD--NDKVEFLWSSEEINSKADKYWPLVMDIARKNKLPRI 184

Query: 183 IRCSQIMGRTEQDELTAAQILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKR 242
           +RC QIMGR+E DEL+AAQILYPCMQCADIFFL+ADICQLGMDQRKVNVLAREYCDDIKR
Sbjct: 185 LRCVQIMGRSETDELSAAQILYPCMQCADIFFLEADICQLGMDQRKVNVLAREYCDDIKR 244

Query: 243 KNKPIILSHHMLPGLQQGQEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCL 302
           KNKPIILSHHMLPGLQQGQEKMSKSDP S+IFMED+EAEVN+KIKKAYCPP +V+GNPCL
Sbjct: 245 KNKPIILSHHMLPGLQQGQEKMSKSDPLSAIFMEDEEAEVNVKIKKAYCPPKVVKGNPCL 304

Query: 303 EYIKYLIFPWFKEFTVERNADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKIL 362
           EYIKY+I PWF EFTVERN ++GGNKT+ +FE++ ADY SG LHPGDLK  L  ALNKIL
Sbjct: 305 EYIKYIILPWFDEFTVERNEEYGGNKTYKSFEDIAADYESGELHPGDLKKGLMNALNKIL 364

Query: 363 EPVRDHFKNDSNAKDLLKRVK 382
           +PVRDHFK D+ AK+LLK++K
Sbjct: 365 QPVRDHFKTDARAKNLLKQIK 379

[Close]

BLAST of CmaCh15G012300 vs. TAIR 10
Match: AT1G28350.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 543.5 bits (1399), Expect = 1.4e-154
Identity = 263/363 (72.45%), Postives = 304/363 (83.75%), Query Frame = 0


Query: 21  DAKVQEPGSSSNSSNQLS--LEEKFKIVRSIGEECIQEDELRNLLEKKPEPVCYDGFEPS 80
           D  +    SSS +  Q+S   E K+KIVRSIGEECIQEDEL+NLL KKP P+CYDGFEPS
Sbjct: 383 DLSINTSASSSAAGLQMSEEAEMKYKIVRSIGEECIQEDELKNLLAKKPAPICYDGFEPS 442

Query: 81  GRMHIAQGVMKTINVNKLTASGCRVKIWIADWFAQLNNKMGGDLKKIETVGRYLIEIWKA 140
           GRMHIAQGVMK  NVNKLT++GC+VKIWIADWFAQLNNK+GGDL++I  VG Y  EIW+A
Sbjct: 443 GRMHIAQGVMKVTNVNKLTSAGCQVKIWIADWFAQLNNKLGGDLERIRVVGEYFKEIWQA 502

Query: 141 VGMDLEGGKVEFLWSSKEINSRASEYWPLVMDIARKNKLPRIIRCSQIMGRTEQDELTAA 200
            GM+    KVEFLW+S EIN + S+YWPLVMDIAR+N L RI+RC QIMGR+E + L+AA
Sbjct: 503 GGMN--NDKVEFLWASDEINGKGSKYWPLVMDIARRNNLRRILRCGQIMGRSETEVLSAA 562

Query: 201 QILYPCMQCADIFFLKADICQLGMDQRKVNVLAREYCDDIKRKNKPIILSHHMLPGLQQG 260
           QILYPCMQCADIF L+ADICQLGMDQRKVN+LAREYC DIKRKNKPIILSHHMLPGLQQG
Sbjct: 563 QILYPCMQCADIFLLEADICQLGMDQRKVNMLAREYCADIKRKNKPIILSHHMLPGLQQG 622

Query: 261 QEKMSKSDPSSSIFMEDDEAEVNLKIKKAYCPPLIVEGNPCLEYIKYLIFPWFKEFTVER 320
           QEKMSKSDPSS+IFMED+EA+VN KI KAYCPP  VEGNPCLEY+KY++ P F EF VE 
Sbjct: 623 QEKMSKSDPSSAIFMEDEEADVNEKISKAYCPPKTVEGNPCLEYVKYIVLPRFNEFKVE- 682

Query: 321 NADHGGNKTFTTFEELVADYASGVLHPGDLKPALSKALNKILEPVRDHFKNDSNAKDLLK 380
           +  +GGNKTF +FE++VADY +G LHP DLK AL KALN  L+PVRDHFK +  AK+LL+
Sbjct: 683 SEKNGGNKTFNSFEDIVADYETGELHPEDLKKALMKALNITLQPVRDHFKTNERAKNLLE 742

Query: 381 RVK 382
           +VK
Sbjct: 743 QVK 742

The following BLAST results are available for this feature:

BLAST of CmaCh15G012300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S9J2	1.1e-169	75.07	Tyrosine--tRNA ligase 1, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At2g3384...	[more]
F4HWL4	1.9e-153	72.45	Tyrosine--tRNA ligase 2, cytoplasmic OS=Arabidopsis thaliana OX=3702 GN=At1g2835...	[more]
Q5UPJ7	1.7e-72	44.64	Tyrosine--tRNA ligase OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=YARS PE=1...	[more]
Q5JF63	4.1e-63	36.53	Tyrosine--tRNA ligase OS=Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12...	[more]
Q8U2H3	5.4e-63	35.47	Tyrosine--tRNA ligase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM...	[more]

BLAST of CmaCh15G012300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HXR2	5.0e-221	100.00	Tyrosine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111468992 PE=3 SV=1	[more]
A0A6J1ETE5	2.3e-218	98.95	Tyrosine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111435806 PE=3 SV=1	[more]
A0A5A7THE1	6.5e-213	94.78	Tyrosine--tRNA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3ATS8	2.1e-211	94.75	Tyrosine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103482753 PE=3 SV=1	[more]
A0A0A0KMK0	3.6e-211	94.23	Tyrosine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G153090 PE=3 SV=1	[more]

BLAST of CmaCh15G012300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022969947.1	1.0e-220	100.00	tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita maxima]	[more]
XP_022929135.1	4.8e-218	98.95	tyrosine--tRNA ligase 1, cytoplasmic isoform X2 [Cucurbita moschata] >KAG6579509...	[more]
XP_023551497.1	1.2e-216	98.69	tyrosine--tRNA ligase 1, cytoplasmic [Cucurbita pepo subsp. pepo]	[more]
KAA0042754.1	1.3e-212	94.78	tyrosine--tRNA ligase 1 [Cucumis melo var. makuwa]	[more]
XP_008437283.1	4.3e-211	94.75	PREDICTED: tyrosine--tRNA ligase 1, cytoplasmic [Cucumis melo]	[more]

BLAST of CmaCh15G012300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G33840.1	8.0e-171	75.07	Tyrosyl-tRNA synthetase, class Ib, bacterial/mitochondrial	[more]
AT1G28350.1	1.4e-154	72.45	Nucleotidylyl transferase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 192..384 e-value: 7.4E-81 score: 273.6
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 53..180 e-value: 7.2E-55 score: 188.1
IPR023617	Tyrosine-tRNA ligase, archaeal/eukaryotic-type	PIRSF	PIRSF006588	TyrRS_arch_euk	coord: 36..384 e-value: 1.8E-145 score: 482.3
IPR002305	Aminoacyl-tRNA synthetase, class Ic	PFAM	PF00579	tRNA-synt_1b	coord: 67..361 e-value: 1.7E-66 score: 224.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	PANTHER	PTHR46264:SF8	BNAANNG11300D PROTEIN	coord: 23..383
None	No IPR available	PANTHER	PTHR46264	TYROSINE-TRNA LIGASE	coord: 23..383
None	No IPR available	SUPERFAMILY	52374	Nucleotidylyl transferase	coord: 44..372

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh15G012300.1	CmaCh15G012300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006418	tRNA aminoacylation for protein translation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004831	tyrosine-tRNA ligase activity
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding